KR20100105138A - Composition for promoting the volume of skin - Google Patents

Abstract

PURPOSE: A composition for promoting differentiation of human mesenchymal stem cells to adipocytes is provided to enhance skin volume by generating lipid droplet. CONSTITUTION: An external use composition for promoting adipocyte differentiation contains extract of Castaneae Semen, Selaginella tamariscina, Aloe vera L., Salvia miltiorrhiza Bunge., Mentha arvensis var. piperascens Makinv., Pinellia ternata (Thunb.) Breit., Triticum aestivum L., Saururus chinensis Baill., Polygala tenuifolia Willd., or Gleditsia sinensis Lam. as an active ingredient.

Description

Composition for promoting the volume of skin

The present invention relates to a composition for promoting differentiation of human mesenchymal stem cells into adipocytes, and more particularly, one or more of extracts of dry, baekbaek, presbyopia, salvia, peppermint, halves, wheat, eochocho, raw paper and flakes. The present invention relates to a cosmetic or cosmetic composition for promoting the differentiation of human mesenchymal stem cells into adipocytes as an active ingredient to generate lipid droplets, thereby increasing the volume of the skin of a specific site.

Now that S-line is in the air, the demands of modern women who are trying to make a great body are increasing. In particular, the soft lines leading to the chest, waist, and hips reveal feminine appeal. The firm, voluminous hips are enough to keep your clothes well-dressed. In addition to young women, a decrease in fat due to metabolic abnormalities such as hormones and blood circulation, which occurs mainly in postmenopausal women, causes women to feel a great loss of femininity. Increasing the volume of adipose tissue is a solution to these very sensitive problems in modern society. Many women try to maintain or increase the volume of skin through plastic surgery with side effects and economic burden. do.

A widely used method to improve the volume of the skin is a fat transplant surgery that collects its own fat and transplants it to each part of the skin. These fat grafts have been showing good results by applying to lip, jaw, and forehead correction, skin shading due to nose shaping and burns or wounds, and areas lost due to cancer resection. However, it has been reported that 40-60% of the graft volume is absorbed back into the body after liposuction (Eremia S et al ., Dermatol. Sur. 2000, 26: 1150-1158; Fulton JE et al ., Dermatol. Clin 2001, 19 (3): 523-530), fat transplantation of skin is thin subcutaneous fat area is less raised a problem of which the cell mass touched, actually the transplanted fat survival and survival expectancy There is a case in which replanting is not necessary. Therefore, there is an increasing demand for a composition that can sufficiently increase the subcutaneous fat layer while replacing lipostomy with these problems.

According to a recent study, the basic structure of the skin is maintained by the subcutaneous fat tissue, and the subcutaneous fat tissue plays a role in determining the volume and strength of the skin. It may be a good solution for maintaining persimmon (Trottier V et al., Stem Cells . 2008 26 (10): 2713-23; Park BS et al ., Dermatol.Surg. 2008, 34 (10): 1323-1326). Adipocytes make up the subcutaneous fat layer under the dermis of the skin, act as a cushion to absorb and protect the organs inside the skin against external heat, and nutrients in the form of triglycerides. It is stored in organelles called lipid droplets and used as an energy source when needed.

Research using such adipocytes has been actively conducted in various fields. Generally used preadipocytes are widely used because they have the property of differentiating into adipocytes by differentiation inducing substances such as insulin. In particular, the recent attention in the study using fat cells mesenchymal stem cells (mesenchymal stem cells), adipocyte derived stem cells (adipocyte derived stem cells). These stem cells can generally be differentiated using methods similar to those used to differentiate adipocytes into adipocytes (Xu Y et al ., Exp. Cell Res. 2006, 312, 1856-1864). It is widely used in the study of adipocyte differentiation (Pittenger MF et al ., Science 1999, 284, 143-146).

Therefore, the present inventors have tried to find a herbal extract that promotes the differentiation of fat cells constituting adipose tissue. When the treatment was found to effectively promote the process of differentiation of mesenchymal stem cells into adipocytes and completed the present invention.

Accordingly, it is an object of the present invention to provide a cosmetic or cosmetic composition for enhancing the volume of the skin by containing a herbal extract that promotes the differentiation of human mesenchymal stem cells into adipocytes.

In order to achieve the above object, the present invention is for promoting the differentiation of fat cells containing one or more extracts selected from the group consisting of dry rate, baekbaek, rosacea, red ginseng, peppermint, half, wheat, eoseongcho, raw paper and sculpting as an active ingredient An external preparation composition and a pharmaceutical composition are provided.

The composition according to the present invention contains one or more of the extracts of dry, baekbaek, rosacea, sweet ginseng, peppermint, halved, wheat, fish vinegar, raw paper and flakes as active ingredients to promote mesenchymal stem cell differentiation into adipocytes. It is useful for increasing adipose tissue for cosmetic or cosmetic purposes and thus helps to increase skin volume.

The present invention provides a composition for promoting adipocyte differentiation, which contains one or more extracts selected from the group consisting of dry percentage, baekbaek, rosacea, sweet ginseng, peppermint, halved, wheat, fish herb, raw paper, and sculpt.

As used herein, the term “fat cell differentiation” refers to the differentiation of adipocytes or mesenchymal stem cells from animals, preferably mammals into adipocytes, and more preferably human mesenchymal stem cells. Preferably, mesenchymal stem cells derived from human bone marrow differentiate into adipocytes.

The herbal extract used in the present invention is as follows.

Castaneae Semen has a peculiar smell, tastes sweet and is warm. The effect is a dry stomach, diarrhea to stop the renal function to encourage the back pain, leg weakness is used. It is also effective in hemostasis and bronchitis. Antioxidant activity has been reported as a pharmacological action, and rich in vitamin C has been reported to be effective in skin beauty, fatigue recovery, cold prevention.

Kwonbaek (卷柏) ( Selaginella tamariscina ) is odorless, tastes very good, and has a flat and unbiased nature. The effect is hemostasis to various bleeding stool bleeding, hemorrhage, urine, uterine bleeding, etc., the raw is effective in blood circulation, no menstruation or dysmenorrhea, bruises. Pharmacological action has been reported to inhibit staphylococcus aureus.

Presbytery ( Aloe vera L.) refers to the whole plant belonging to the genus Aloe or to one species. It is native to Africa and has about 300 species worldwide. As a houseplant, it can be grown in a greenhouse or grown at home for use as a medicine. It contains aloetin, which is bactericidal and fungicidal to bacteria and fungi, and contains aloeursin, which is effective against ulcers, and aloemitin, which has anticancer effects. In addition, it contains various ingredients such as steroids, amino acids, saponins, antibiotics, wound healing hormones and minerals. Aloe is effective in recovering from fatigue caused by overwork and relieving hangovers caused by excessive drinking, and cutting aloe leaves extraordinarily bitter yellow material, which is particularly effective in constipation. In the private sector, aloe leaf juice is used for gastrointestinal diseases and used for trauma and burns. It also neutralizes dry and oily skin and moisturizes the skin, making it a cosmetic ingredient.

Peppermint ( Mentha arvensis var. Piperascens Makinv.) Has a peculiar smell and it is spicy and cold if you put it in your mouth and feel cool. Efficacy is used for fever, headache, sweating due to external cold, eye redness, sore throat, tonsillitis, measles, early skin itching, liver disease, abdominal pain, diarrhea, vomiting. Pharmacological actions include fever, anti-inflammatory, dry stomach, bile secretion and gastrointestinal smooth muscle suppression, increased mucus secretion in the respiratory tract, central nervous excitability, and uterine contractions.

Pinellia ternata (Thunb.) Breit. Is almost odorless and slightly mucus when chewed, but later terribly sick, tastes hot, warm and poisonous. The effect is to treat sputum, so it is used for phlegm, sea water, asthma, headache, dizziness, puffiness, full symptoms, vomiting, sore throat, boils on the back, mastitis, pregnancy and vomiting. Pharmacological action was reported as expectoration, Jinhae, nausea and soothing, silicosis prevention, anticancer activity.

Wheat (小麥) ( Triticum aestivum L.) is also known as wheat. Wheat is a staple food in the East but a staple food in the West, along with rice, making it the world's second largest food crop. More than 90% are milled and used for noodle making, baking, confectionery and industrial purposes. It is also used as a raw material for soy sauce and miso, and bran is a good feed. Straw is chewy and thin, which is used to make straw hats.

Saururus chinensis Baill. Is a fishy fishy taste that is spicy and slightly cold. Effective for antipyretic, drainage, cough due to lung abscess, pus, vomiting, pneumonia, acute bronchitis, enteritis, urinary tract infections, boils, fever and urine can not be used. Pharmacological action, antimicrobial action, immune enhancing action, anti-inflammatory action, diuretic action, antitussive action has been reported.

Polygala tenuifolia Willd. Is thick and long with roots, with several stalks at the ends and almost without hairs. Leaves are alternate, row-shaped, 1.5-3cm long, without petioles. Petals are open at the top, attached at the bottom, and chopped like a brush. Fruits are capsules, flat, split into two, and seeds have dense hairs. In oriental medicine, the root is called Wonji and it is used as expectorant, tonic and gangjeong. It is distributed in the north of central Korea and north of China.

Gleditsia sinensis Lam. Is a herb used in various folk remedies such as improving intestinal function in East Asia. Thorn is called a sculptor, and it has the effect of sowing, drainage, etc., used in various boils. Overwintering is also possible in central Korea.

For sometimes called Salvia (丹蔘) (Salvia miltiorrhiza Bunge .) Is jeoksam (赤參), it had a slight odor and yakseong writes tteolp little car. The effect is to help circulate blood, remove blood and relieve joint pain. Menstrual dysmenorrhea, menstrual pain, postpartum abdominal pain, bloody heartache, bruises, etc. are treated. It is used to treat mental disorders, bullshit, annoyance, insomnia, skin rash, palpitations due to high fever. Pharmacological actions include coronary dilatation, lower cholesterol, lower blood pressure, hepatic function activation, soothing anti-inflammatory, anticancer and antibacterial effects.

The extracts used in the present invention can be extracted by conventional extraction methods well known in the art using organic solvents such as water, ethanol, pepper, salvia, salvia, peppermint, halved, wheat, fish herb, raw paper and flakes. Can be.

The composition of the present invention promotes the differentiation of mesenchymal stem cells (MSCs), constitutes a subcutaneous fat layer or provides fat cells to impart a sense of volume to the skin, and may be provided as an external skin preparation composition or a pharmaceutical composition. have.

When the composition according to the present invention is used as a skin external preparation composition, it is not particularly limited in the formulation according to the body part. Specifically, for example, it may be a cosmetic composition having a formulation of softening water, nourishing cream, massage cream, nourishing cream, pack, gel or skin adhesive type cosmetics, and also includes lotions, ointments, gels, creams, patches or sprays. Same transdermal dosage form.

Skin external preparation composition according to the present invention is an active ingredient with one or more extracts selected from the group consisting of dryness, whitening, rosacea, red ginseng, peppermint, half, wheat, eoseongcho, raw paper and sculpting as an active ingredient 0.0001 ~ It is contained in an amount of 30% by weight.

In addition, in the composition for external application for skin according to each formulation, other ingredients other than the extracts which are the dryness, whitening, rosacea, salvia, salvia, peppermint, halved, wheat, vinegar, raw paper or flakes of the present invention are used for the preparation or use of other skin external preparations. Those skilled in the art may suitably select and mix the materials without difficulty, and in this case, synergistic effects may occur when applied simultaneously with other raw materials.

In addition, when the composition according to the present invention is used as a pharmaceutical composition, it further contains a pharmaceutical adjuvant such as preservatives, stabilizers, hydrating or emulsifying accelerators, salts and / or buffers for controlling osmotic pressure and other therapeutically useful substances. And may be formulated in various oral or parenteral dosage forms according to conventional methods.

Formulations for oral administration include, for example, tablets, pills, hard and soft capsules, solutions, suspensions, emulsifiers, syrups, granules, etc. These formulations may contain diluents (e.g. lactose, dextrose, sucrose, Mannitol, sorbitol, cellulose and glycine) and glidants such as silica, talc, stearic acid and its magnesium or calcium salts and polyethylene glycols. Tablets may also contain binders such as magnesium aluminum silicate, starch paste, gelatin, tragacanth, methylcellulose, sodium carboxymethylcellulose and polyvinylpyrrolidine, optionally starch, agar, alginic acid or its sodium salt Pharmaceutical additives such as disintegrants, absorbents, colorants, flavors, and sweeteners. Tablets may be prepared by conventional mixing, granulating or coating methods. Also representative of parenteral formulations are injectable formulations, preferably aqueous isotonic solutions or suspensions. However, it should be understood that the actual dosage of the active ingredient should be determined in view of several relevant factors such as the severity of the symptom, the route of administration chosen, the subject's age, sex, weight and health condition, and therefore the dosage of the herbal extracts. Does not limit the scope of the invention in any way. Generally, the dosage of the herbal extracts ranges from 0.001 mg / kg / day to approximately 2000 mg / kg / day. More preferred dosages are from 0.5 mg / kg / day to 2.5 mg / kg / day.

The site of application of the cosmetic or cosmetic composition for promoting the differentiation of mesenchymal stem cells according to the present invention includes all the adipose tissue of the body, but is not limited to a specific site, for example, breasts, hips of women with a lot of fat layers Site may be included. The increasing effect of the adipose tissue, but the effect of increasing the adipose tissue for cosmetic or cosmetic purposes, but is not limited thereto.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the following examples are only for illustrating the present invention, and the scope of the present invention is not limited thereto.

Example 1 Preparation of Herbal Extract

30g each of dryness, baekbaek, presbytery, dansam, peppermint, half, wheat, eoseongcho, raw paper, sculptor and male, white paper, baekduong, songjeol, brain, hyssop, flax, benzoin (95.0 ~ 95.5v / v%) ), And put into 200ml and pulverized for 20 minutes under pressure of 1500psi at 50 ℃ using ASE300 ACCELERATED SOLVENT EXTRACOR (DIONEX Corporation) instrument. In addition, using an ultrasonic cleaner (BRANSON Ultrasonics corporation) sonicator device was further ground for 3 days at 50 ℃. Each of the pulverized extracts were dried at 40 ° C. for 24 hours using a module spin 40 (Modul spin 40; Biotron corporation) instrument to prepare each extract sample.

Experimental Example 1 Efficacy and Quantitative Measurement of Human Bone Marrow-derived Mesenchymal Stem Cells into Adipocytes

Human bone marrow mesenchymal stem cells (hBM-MSCs) were purchased from Lonza, Inc. Walkersville, MD, USA and cultured according to Lonza's instructions. Adipocyte differentiation was achieved using the method recommended by Lonza, except that Troglitazone was used instead of indomethacin to induce adipocyte differentiation of mesenchymal stem cells. Specifically, 1 μg / ml of insulin, 1 μM of dexamethasone (DEXA) and 0.5 mM of isobutyl methyl xanthine (IBMX) were cultured in the culture of mesenchymal stem cells to differentiate stem cells into adipocytes. At the time of induction of adipocyte differentiation, extracts of dry, baekbaek, rosacea, salvia, peppermint, halved, wheat, estuary, raw paper or flakes were added to the medium under IDXT conditions. In addition, for comparison, 10 ppm each of male, white paper, baekduong, songjeol, yong brain, hyssop, flax, or benzoin extract were added to the medium. As a positive control, 2 μM of Troglitazone (TRO) was used and treated with IDX medium. After 14 days of adipocyte differentiation of mesenchymal stem cells, the cell culture was removed, the cells were washed with 10 ml of Phosphate Buffered Saline (PBS), and then 0.2 ml / cm of 10% formalin / PBS solution. Cells were fixed for 10 minutes using ² . The fixed cells were washed once with 0.5 ml / cm ² of 60% isopropanol solution, and stained with lipids in adipocytes with 0.2 ml / cm ² stained with Oil Red O in 60% isopropanol for 10 minutes. It was. Subsequently, re-staining with hematoxylin to make cell nuclei easily distinguished and then observed under a microscope, and treated with extracts of dryness, baekbaek, rosacea, sweet ginseng, peppermint, halves, wheat, vinegar, grass or slices. The results are shown in FIG.

Figure 1 shows that the differentiation of adipocytes when adipocyte differentiation of the mesenchymal stem cells was added under IDXT conditions and then the adipocyte differentiation of mesenchymal stem cells was induced under IDXT conditions. Can be seen to be more effective.

After dyeing the oil redo with 100% isopropanol solution, the absorbance was measured at a wavelength of 500 nm, the results are shown in Figures 2a to 2h. At this time, each data value was corrected with an insulin control. This increase was statistically significant, and the statistics were verified by the two-tailed Student's t test, indicating that there was a significant difference when the p value was less than 0.05. In addition, IDXT of mesenchymal stem cells when dry, baekbaek, presbytery, dansam, peppermint, half, wheat, eoseongcho, raw paper, sculpted, male, white paper, baekduong, songjeol, brain, dew, flax, and benzoin extracts Compared with the increase in fat differentiation showed more than 30%, it is shown in Table 1

Herbal medicine Scientific name IDXT vs. Growth Rate Dry rate Castaneae semen 30% Kwonbaek Selaginella tamariscina 40% craftiness Aloe 47% Sculpture Gleditsiae semen 31% Salvia Salviae Radix 38% Mint (Sat) Menthae Herba 33% Half Pinelliae tuber 38% Wheat Tritici Semen 31% Paper Polygalae radix 37% Eoseongcho (Chinese medicine) Houttuyniae Herba 34% male Arisaematis Rhizoma 0.017% blank Angelicae Dahuricae Radix 6% reduction Baekduong Pulsatillae radix 19% Songjeol Pini Ramulus 25% Brain Borneolum 0.08% Dew Achuranthis radix 0.1% Flax Lini Semen 3.5% reduction Benzoin Enzoinum 21%

As can be seen from the results of Table 1 and Figures 2a to 2h, extracts of dryness, rolled rice, rosacea, sweet ginseng, peppermint, halved, wheat, eochocho, raw paper or flakes according to the present invention, respectively, apoptosis of mesenchymal stem cells Promote the differentiation of adipocytes without induction, and have been shown to promote the differentiation of adipocytes much more effectively than men, white paper, baekduong, songjeol, yong brain, hyssop, flax, or benzoic extracts. Can be.

Test Example 2 Measurement of Skin Volume Enhancement Effect

In order to investigate the effect of increasing the volume of human skin on the use of dry percentage, baekbaek, rosacea, salvia, mint, peppermint, wheat, eochocho, raw paper or sculptural extract of the present invention, a survey was conducted on 15 clinical trial participants. .

Specifically, each of the extracts, as shown in Table 2, was applied to the facial cheek area once a day for 8 weeks with a nourishing cream containing 2.5% by weight of the total weight of the composition, and then questioned about the improvement of the volume. Investigation was conducted. In the case where the effect of improving the volume is good, ○, the normal case is indicated by △, and the case where there is no effect is indicated by ×, and the results are shown in Table 3 below.

Compounding ingredient Content (% by weight) Purified water To 100 Extract of dried, baekbyeon, rosacea, sweet ginseng, peppermint, half, wheat, fish 2.5 Vegetable Cured Oil 1.5 Stearic acid 0.6 Glycerol Stearate 1.0 Cetheanyl alcohol 2.0 Polyglyceryl-10 Pentastearate & Behenyl Alcohol & Sodium Stearoyl Lactylate 1.0 Arachidil Behenyl Alcohol 1.0 Cetylaryl Alcohol & Cetearyl Glucoside 2.0 PEG-100 stearate & glycerol oleate & propylene glycol 1.5 Caprylic / Carlic Triglycerides 11.0 Cyclomedicon 6.0 Preservative, incense Quantity Triethanolamine 0.1

Herbal medicine Volume increase effect (person) ○ △ × Dry rate 10 2 3 Kwonbaek 9 4 2 craftiness 11 2 2 Salvia 8 4 3 mint 8 5 2 Half 12 2 One Wheat 9 2 4 Eoseongcho 10 3 2 Paper 12 One 2 Sculpture 9 3 3 blank 2 4 9

As can be seen in Table 3, when the white paper extract is used, the effect of improving skin volume is not good, whereas the dryness, rolled white, rosacea, salvia, peppermint, halved, wheat, eochocho, raw paper or flake extract of the present invention. When used, the effect of increasing skin volume was found to be good for most participants.

From the results of the experiment, the composition containing the extract of dryness, baekbaek, rosacea, sweet ginseng, peppermint, halved, wheat, estuary, raw paper or flakes is applied to the skin of the face, and the active ingredient of the extract is fat It can be seen that the volume of the tissue is increased, thereby increasing the volume and elasticity of the skin.

Examples of formulations for the composition of the present invention are illustrated below.

Preparation Example 1 Tablet

After mixing 100mg of extract of one of the dryness, rolled rice, rosacea, sweet ginseng, peppermint, halved, wheat, fish vinegar, raw paper, and flakes according to the present invention, 400 mg of lactose, 400 mg of corn starch, and 2 mg of magnesium stearate, Tablets were prepared by tableting according to the preparation method.

Preparation Example 2 Capsule

After mixing 100mg of extract of one of dryness, rolled rice, rosacea, red ginseng, peppermint, halved, wheat, fish vinegar, raw paper and flakes, 400mg of lactose, 400mg of corn starch, and 2mg of magnesium stearate, Therefore, the capsules were prepared by filling in gelatin capsules.

[Example 3] Softening Cosmetic (Skin Lotion)

Compounding ingredient Content (% by weight) Purified water To 100 Extract of one of dry rate, baekbaek, rosacea, sweet ginseng, peppermint, half, wheat, eoseongcho, raw paper and sculptural 0.1-0.2 Butylene glycol 2.0 Propylene glycol 2.0 Carboxy Vinyl Polymer 0.1 Fiji-12 nonylphenyl ether 0.2 Polysorbate 80 0.4 ethanol 10.0 Triethanolamine 0.1 Preservative, coloring, fragrance Quantity

[Example 4] Nutritional Longevity (Milk Lotion)

Compounding ingredient Content (% by weight) Purified water To 100 Extract of one of dry rate, baekbaek, rosacea, sweet ginseng, peppermint, half, wheat, eoseongcho, raw paper and sculptural 0.1-0.2 Beeswax 4.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.5 Liquid paraffin 0.5 Caprylic / capric triglyceride 5.0 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxy Vinyl Polymer 0.1 Triethanolamine 0.2 Preservative, coloring, fragrance Quantity

[Example 5] Nutritional Cream

Compounding ingredient Content (% by weight) Purified water To 100 Extract of one of dry rate, baekbaek, rosacea, sweet ginseng, peppermint, half, wheat, eoseongcho, raw paper and sculptural 0.1-0.2 Beeswax 10.0 Polysorbate 60 1.5 Sebum 60 Cured Castor Oil 2.0 Sorbitan sesquioleate 0.5 Liquid paraffin 10.0 Squalane 5.0 Caprylic / capric triglyceride 5.0 glycerin 5.0 Putylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 Preservative, coloring, fragrance Quantity

[Example 6] Massage Cream

Compounding ingredient Content (% by weight) Purified water To 100 Extract of one of dry rate, baekbaek, rosacea, sweet ginseng, peppermint, half, wheat, eoseongcho, raw paper and sculptural 0.1-0.2 Beeswax 10.0 Polysorbate 60 1.5 Sebum 60 Cured Castor Oil 2.0 Sorbitan sesquioleate 0.8 Liquid paraffin 40.0 Squalane 5.0 Caprylic / capric triglyceride 4.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 Preservative, coloring, fragrance Quantity

[Example 7] Pack

Compounding ingredient Content (% by weight) Purified water To 100 Extract of one of dry rate, baekbaek, rosacea, sweet ginseng, peppermint, half, wheat, eoseongcho, raw paper and sculptural 0.1-0.2 Polyvinyl alcohol 13.0 Sodium Carboxymethyl Cellulose 0.2 glycerin 5.0 Allantoin 0.1 ethanol 6.0 Fiji-12 nonylphenyl ether 0.3 Polysorbate 60 0.3 Preservative, coloring, fragrance Quantity

FIG. 1 is a photograph showing microscopic observation of adipocyte differentiation of mesenchymal stem cells under IDXT, after treatment with Salvia helium extract under IDXT, and after IDXT.

Figure 2a ~ 2h is a graph showing the relative fat differentiation value compared to IDXT of mesenchymal stem cells after the treatment of extracts of dryness, baekbaek, rosacea, sweet ginseng, peppermint, halved, wheat, eoseongcho, raw paper or flakes.

Claims (9)

Skin external composition for promoting fat cell differentiation comprising one or more extracts selected from the group consisting of dry rate, baekbaek, rosacea, sweet ginseng, peppermint, halved, wheat, eoseongcho, raw paper and sculpt. The composition for external application for skin according to claim 1, wherein the composition promotes differentiation of mesenchymal stem cells into adipocytes. The composition for external application of skin according to claim 2, wherein the mesenchymal stem cells are human mesenchymal stem cells. 3. The composition for external application for skin according to claim 2, wherein the composition increases fat tissue. According to claim 1, wherein the composition is 0.0001 to 30% by weight relative to the total weight of the at least one extract composition selected from the group consisting of dry, rolled, rosacea, sweet ginseng, peppermint, half, wheat, fish vinegar, raw paper, and carpgin An external preparation composition for skin, characterized in that it contains. The external preparation composition for skin according to claim 1, wherein the composition increases skin volume. A pharmaceutical composition for promoting the differentiation of fat cells, containing one or more extracts selected from the group consisting of dry rate, baekbaek, rosacea, red ginseng, peppermint, halved, wheat, eoseongcho, raw paper and sculpt. 8. The pharmaceutical composition of claim 7, wherein the composition promotes differentiation of mesenchymal stem cells into adipocytes. 8. The composition of claim 7, wherein the composition has a dosage of one or more extracts selected from the group consisting of dry, rolled, rosacea, red ginseng, peppermint, half, wheat, fish vinegar, raw paper, and flakes from 0.001 mg / kg / day to 2000 mg / day. Pharmaceutical composition characterized in that the kg / day.

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