CN109876012A - A kind of Pharmaceutical composition and its application in promotion skin wound healing - Google Patents
A kind of Pharmaceutical composition and its application in promotion skin wound healing Download PDFInfo
- Publication number
- CN109876012A CN109876012A CN201910215368.2A CN201910215368A CN109876012A CN 109876012 A CN109876012 A CN 109876012A CN 201910215368 A CN201910215368 A CN 201910215368A CN 109876012 A CN109876012 A CN 109876012A
- Authority
- CN
- China
- Prior art keywords
- parts
- pharmaceutical composition
- weight
- traditional chinese
- chinese drug
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to a kind of Pharmaceutical composition and its promoting the application in skin wound healing.The Pharmaceutical composition includes mescenchymal stem cell and Traditional Chinese drug mixture, and wherein the Traditional Chinese drug mixture includes aloe and peppermint.The powerful proliferation and differentiation capability that this Pharmaceutical composition utilizes mescenchymal stem cell to have, and it is aided with the application in TCM such as aloe and peppermint in skin wound healing, in the period that can shorten to significant effective wound healing, the formation of scar is reduced, Treatment need is met.
Description
Technical field
The present invention relates to Wound treating technical field, especially a kind of Pharmaceutical composition and its promote skin wound healing
In application.
Background technique
In daily life, the skin of people can inevitably be scratched, burns or scald.Skin healing is one suitable
Process that is complicated and being participated in by many factors collaboration, needs to be formed by inflammatory reaction, granulation tissue hyperplasia and epithelial tissue
Stage, healing cycle are generally 21 days or so.For lighter wound situation, patient often with the drugs such as antiphlogistic and bactericidal, analgesic come
Processing, then wound can heal naturally.However, when traumatic event is serious, when such as there is scald, burn, skin wound depth
It is larger with area, it itself is difficult to quickly heal, so that patient is born more lasting feeling of pain, and treatment cycle is elongated and easy
There is wound superinfection, medical expense increases, and can also generate biggish scar after wound healing, lose skin gloss and
Flexibility influences skin overall appearance, and very big psychological shade is caused to people.Although the drug such as medicine for external application that uses of tradition and
There are many ointment type, but have no currently on the market and be specifically used for promoting wound healing and reduce the drug of scar, therefore
It is not able to satisfy the demand treated wound.
Summary of the invention
In view of the above-mentioned problems, the present invention provides a kind of Pharmaceutical composition and its application in promotion skin wound healing,
Wherein the Pharmaceutical composition includes mescenchymal stem cell and Traditional Chinese drug mixture.Mescenchymal stem cell is a kind of multipotential stem cell,
With powerful proliferative capacity and Multidirectional Differentiation ability, after being externally applied at skin wound, can be divided into dermal tissue with
Improve the healing rate of the surface of a wound.Meanwhile Traditional Chinese drug mixture contains the substances such as aloe and peppermint, aloe is containing there are many to skin beneficiating
The effect of ingredient, such as aloin, wound hormone and glycan peptide sweet dew (Ke-2), with antibacterial anti-inflammatory, hemostasia and detumescence and rush
The effect restored into wound healing, the skin cell tissue to die can be disintegrated, allow dead skin to fall off naturally to reduce wound and be cured
Generated scar after conjunction.In addition, effect ingredient contained by another main component peppermint is menthol, when applied to skin
When wound, it can make to stimulate human nerve tip to generate refrigerant and antalgesic-antipruritic effect.In order to accelerate wound healing simultaneously
Less scar the present invention provides a kind of Pharmaceutical composition and its is promoting the application in skin wound healing.Mesh of the invention
Be achieved through the following technical solutions:
A kind of Pharmaceutical composition, the Pharmaceutical composition includes following component: mescenchymal stem cell, Traditional Chinese drug mixture；Its
In, the Traditional Chinese drug mixture contains aloe, peppermint；Preferably, it on the basis of the total weight of described pharmaceutical composition, is filled between described
Matter stem cell is 5-10 parts, and the Traditional Chinese drug mixture is 15-20 parts；Preferably, using the total weight of the Traditional Chinese drug mixture as base
Standard, the aloe is 30-60 parts by weight, the peppermint is 10-15 parts by weight.
Further, the Pharmaceutical composition further includes one of human serum albumin, Low-molecular-weight Heparins Calcium and vitamin C
Or it is several.
Further, the Pharmaceutical composition further includes human serum albumin, Low-molecular-weight Heparins Calcium and vitamin C.Medicinal group
Nutrition can be supplied for stem cell by closing the human serum albumin in object, to maintain the quantity and motility rate of stem cell, low molecular weight heparin
Calcium have prevent cell aggregation and cell made to be evenly distributed in drug matrices, can guarantee in this way Pharmaceutical composition dosage and
Drug effect, and both ingredients are clinical commonly used drugs, thus will not influence human body use it is safe.In addition, vitamin C can maintain
The activity of peroxidase can be reserved for the activity of cell.
Further, the Pharmaceutical composition further includes one or more of ointment, preservative, moisturizer and water；
Preferably, the Pharmaceutical composition further includes ointment, preservative, moisturizer and water.
Further, the Traditional Chinese drug mixture further includes one in the fruit of summer cypress, the fleece-flower root, beeswax, Asiatic plantain and dandelion
Kind is several.
Further, the Traditional Chinese drug mixture further includes the fruit of summer cypress, the fleece-flower root, beeswax, Asiatic plantain and dandelion.Chinese medicine is mixed
Closing the other compositions such as fruit of summer cypress, the fleece-flower root in object has effects that pruitus, and beeswax has removing toxic substances, sore, myogenic pain relieving
The effect of, dandelion and Asiatic plantain have clearing heat and detoxicating a, effect of dispersing swelling and dissipating binds, and the comprehensive use of this five kinds of traditional Chinese medicine ingredients can be into
One step assist wound quickly heals, and mitigates the feeling of pain of patient.
Preferably, the mescenchymal stem cell is human umbilical cord mesenchymal stem cells.
Further, the Pharmaceutical composition includes following component: human umbilical cord mesenchymal stem cells, human serum albumin, low
Molecular heparin calcium, vitamin C, Traditional Chinese drug mixture, ointment, preservative, moisturizer and water；The Traditional Chinese drug mixture includes reed
Luxuriant growth, peppermint, the fruit of summer cypress, the fleece-flower root, beeswax, Asiatic plantain, dandelion；Preferably, using the total weight of described pharmaceutical composition as base
Standard, the human umbilical cord mesenchymal stem cells are 5-10 parts, and the human serum albumin is 1-3 parts, and the Low-molecular-weight Heparins Calcium is
0.1-0.2 parts, the vitamin C is 0.3-0.5 parts, and the Traditional Chinese drug mixture is 15-20 parts, and the ointment is 20-30 parts,
The preservative is 0.2-0.6 parts, and the moisturizer is 1-1.5 parts, and the water is 86-93 parts；Wherein, with Chinese medicine mixing
On the basis of the total weight of object, the aloe is 30-60 parts by weight, the peppermint is 10-15 parts by weight, the fruit of summer cypress is 8-15
Parts by weight, the fleece-flower root are 10-18 parts by weight, the beeswax is 5-10 parts by weight, the Asiatic plantain is 3-8 parts by weight, institute
Stating dandelion is 8-15 parts by weight.
Preferably, the ointment is glycerol；The preservative is selected from sorbic acid, butoben and nisin
One of rhzomorph is a variety of；The moisturizer is selected from one of butanediol, sorbierite and Sodium Hyaluronate or a variety of.
All traditional Chinese medicinal components of Pharmaceutical composition of the present invention are powder type, and the thinner powder the better.
The present invention also provides a kind of method for preparing the Pharmaceutical composition, this method includes by the institute of the Pharmaceutical composition
There is component to be uniformly mixed.For water-free Pharmaceutical composition, when in use, suitable quantity of water can be added and be tuned into Pharmaceutical composition
Paste.
Preferably, the Pharmaceutical composition is prepared according to the following steps: (1) will be in addition to human umbilical cord mesenchymal stem cells
Other compositions are uniformly mixed；(2) human umbilical cord mesenchymal stem cells are added in the substance of step (1) acquisition.
The invention has the advantages that the main component mescenchymal stem cell in the Pharmaceutical composition, such as between people's umbilical cord
Mesenchymal stem cells both can directly provide reparation as a kind of stem cell with multi-lineage potential and powerful paracrine effect
Cell promotes to damage wound healing, accelerates repairing cell proliferation rate, can also secrete various anti-inflammatory factors to inhibit inflammation anti-
Should with play anti-inflammatory effect, and also can induce endogenous endothelial growth factor expression, promote capillary proliferation, repairing damage
Blood vessel, and production quantity and its amount for generating extracellular matrix by inhibiting Wound fibroblasts adjust wound healing,
Reduce cicatrization.Human umbilical cord mesenchymal stem cells are easy to draw materials simultaneously, without prejudice to ethics, and have low immunogenicity,
Without apparent rejection after being transplanted in vivo, biological safety is higher.Furthermore the Chinese medicine in the Pharmaceutical composition contains
Aloe, peppermint, the fruit of summer cypress, the fleece-flower root, beeswax, Asiatic plantain, dandelion, these ingredients have sterilization, anti-inflammatory, detumescence and analgesic etc.
Effect.Therefore, the synergistic effect of Multiple components can reduce to significant effective the time of wound healing in the Pharmaceutical composition, have
The quickening treatment speed of effect, and the slight illness of patient is reduced, the formation of scar is reduced, Treatment need is met.
Specific embodiment
Below with reference to embodiment, the content of the present invention will be explained in more detail.It should be understood that implementation of the invention is not limited to
In the following examples, the accommodation in any form and/or change made to the present invention fall within protection model of the invention
It encloses.
In the present invention, if not refering in particular to, all parts, percentage are unit of weight, and all equipment and raw material etc. are equal
It is commercially available or the industry is common.If the method that embodiment uses is technology hand commonly used in the art without specializing
Section.
Embodiment 1
1. the preparation of human umbilical cord mesenchymal stem cells
It aseptically takes umbilical cord to be cut into 3cm or so segment, takes out abandoning blood vessel and first used after taking blood vessel week substance to shred
Then 1 ‰ IV Collagenase Type is centrifuged 10min under the revolving speed of 2000r/min, lower sediment is taken to use 0.25% again in digestion 2h
Trypsase-PBS continues to digest 25min at 37 DEG C, is then centrifuged 10min under the revolving speed of 2000r/min, cell precipitation is taken to use
After the digestion of 0.25% trypsin solution, then under the revolving speed of 800r/min it is centrifuged 10min, takes cell precipitation with 1 × 106A/ml
Density suspension in culture medium, medium component be commercialization commercially available mesenchymal stem cell serum-free culture medium.Set 37 DEG C,
100% humidity, 5%CO2With cultivated under dark surrounds, change within every 3 days liquid 1 time, it is visible raw in bottom of bottle at fibrous cell through 2 days
It is long, can reach after about 10 days 80% digested with 0.25% trypsase-PBS when converging after, then with cell density 5 × 104A/cm2
Secondary culture.
2. the detection of the biological property of human umbilical cord mesenchymal stem cells
A. flow cytometer cellular elements surface markers are identified:
It takes the good 3rd generation human umbilical cord mesenchymal stem cells of growth conditions to do flow cytometry, sees whether it expresses
The surface moleculars such as CD34, CD44, CD45, CD73, CD90, CD105, HLA-DR.
The results show that expression interstitial cell surface marker CD44, CD73, CD90, CD105, do not express hematopoietic cell mark
CD34, CD45 are not expressed and are repelled the surface marker HLA-DR for reacting closely related with trnasplantion immunity.
B. immunofluorescent staining is tested:
The good 3rd generation human umbilical cord mesenchymal stem cells of growth conditions are taken, cell concentration is adjusted, does cell climbing sheet, cell
It is fixed after covering with 4% paraformaldehyde, does immunofluorescent staining, whether detection contains fluorescence microscopy into the cell under the microscope
Vimentin。
The results show that cells express cell skelemin Vimentin.
C. external Multidirectional Differentiation experiment:
Human umbilical cord mesenchymal stem cells are lured in dexamethasone, the skeletonization such as β-phosphatide glycerol, ascorbate after secondary culture
It is cultivated in conductor system to osteoblast differentiation, is identified with alizarin red and alkaline phosphatase staining；It is filled between secondary culture descendant's umbilical cord
Matter stem cell is cultivated in the adipogenic induction system containing 3-isobutyl-1-methylxanthine, indoles magnesium zinc to lipoblast
Differentiation, is identified with oil red O stain.
The results show that human umbilical cord mesenchymal stem cells can be divided into osteoblast and lipoblast.
3. the preparation of Pharmaceutical composition
A. on the basis of Pharmaceutical composition total weight: 3 parts of human serum albumins, 0.2 part of Low-molecular-weight Heparins Calcium, 0.5 part of dimension life
Plain C, 20 parts of Traditional Chinese drug mixtures, 30 parts of ointments, 0.6 portion of preservative, 1.5 parts of moisturizer and 93.9 parts of water are placed in blender
It is stirred；Wherein, on the basis of the total weight of Traditional Chinese drug mixture, Traditional Chinese drug mixture includes 30 parts of Aloses, 10 portions of peppermints
Powder, 8 parts of Belvedere Fruits, 18 parts of fleece-flower root powder, 10 parts of beeswax powder, 8 parts of Plantain Herbs, 15 parts of dandelion powder.
In addition, ointment is glycerol；Preservative be sorbic acid, hydroxy benzenes first butyl ester and nisin mixture, and sorbic acid,
The weight ratio of hydroxy benzenes first butyl ester and nisin is 2:2:3；Moisturizer is butanediol, sorbierite and Sodium Hyaluronate
Mixture, and the total amount ratio of butanediol, sorbierite and Sodium Hyaluronate is 1:1:2.
Mixing time is 2 hours, and the mixing direction of change in every 4 minutes, stirring obtains paste object after completing.
B. 10 parts of human umbilical cord mesenchymal stem cells on the basis of Pharmaceutical composition total weight are injected into step a and are made
The paste object in, and 37 DEG C at a temperature of stir, mixing time 5min, stirring complete after.
Embodiment 2:
Pharmaceutical composition is prepared in the same manner as in the example 1.The difference is that with the gross weight of Traditional Chinese drug mixture
On the basis of amount, Traditional Chinese drug mixture includes 13 parts of Belvedere Fruits, 12 parts of fleece-flower root powder, 8 parts of beeswax powder, 4 parts of Chinese herbaceous peony grass meals
End, 9 parts of dandelion powder.In addition, ointment is glycerol, preservative is the mixing of butoben and nisin
Object, and the weight ratio of butoben and nisin is 2:3, moisturizer is the mixed of sorbierite and Sodium Hyaluronate
Object is closed, and the weight ratio of sorbierite and Sodium Hyaluronate is 1:2.
In addition, mixing time is 4 hours, the mixing direction of change in every 6 minutes, stirring obtains paste after completing
Body.
Embodiment 3:
Pharmaceutical composition is prepared in the same manner as in the example 1.The difference is that the Pharmaceutical composition includes,
On the basis of the total weight of Pharmaceutical composition: 5 parts of human umbilical cord mesenchymal stem cells, 1 part of human serum albumin, Low-molecular-weight Heparins Calcium
0.1 part, 0.3 part of vitamin C, 15 parts of Chinese medicine, 20 parts of ointment, 0.2 part of preservative, 1 part of moisturizer, 86.8 parts of water, wherein
Traditional Chinese drug mixture is on the basis of its total weight, including 37 parts of Aloses, 12 parts of peppermint powder, 11 parts of Belvedere Fruits, 15 parts
Fleece-flower root powder, 8 parts of beeswax powder, 5 parts of Plantain Herbs, 12 parts of dandelion powder.In addition, ointment is glycerol, preservative
For sorbic acid, moisturizer is butanediol.
Embodiment 4:
Pharmaceutical composition is prepared in the same manner as in the example 1.The difference is that the Pharmaceutical composition includes,
On the basis of the total weight of Pharmaceutical composition: 8 parts of human umbilical cord mesenchymal stem cells, 2 parts of human serum albumin, Low-molecular-weight Heparins Calcium
0.15 part, 0.4 part of vitamin C, 18 parts of Chinese medicine, 25 parts of ointment, 0.4 part of preservative, 1.2 parts of moisturizer, 90 parts of water.Wherein,
Traditional Chinese drug mixture is on the basis of its total weight, including 60 parts of Aloses, 15 parts of peppermint powder, 15 parts of Belvedere Fruits, 10 parts
Fleece-flower root powder, 5 parts of beeswax powder, 3 parts of Plantain Herbs, 8 parts of dandelion powder.In addition, ointment is glycerol, preservative is
The mixture of sorbic acid and nisin, and the weight ratio of sorbic acid and nisin is 2:3, moisturizer is fourth two
The mixture of pure and mild Sodium Hyaluronate, and the weight ratio of butanediol and Sodium Hyaluronate is 1:2.
Embodiment 5
Pharmaceutical composition is prepared in the same manner as in the example 1.The difference is that the Pharmaceutical composition includes,
On the basis of the total weight of Pharmaceutical composition: 5 parts of human umbilical cord mesenchymal stem cells, 20 parts of Chinese medicine, 90 parts of water.Wherein, Chinese medicine is mixed
Object is closed on the basis of its total weight, including 60 parts of Aloses, 10 parts of peppermint powder.
Embodiment 6
Pharmaceutical composition is prepared in the same manner as in the example 1.The difference is that the Pharmaceutical composition includes,
On the basis of the total weight of Pharmaceutical composition: 10 parts of human umbilical cord mesenchymal stem cells, 15 parts of Chinese medicine, 90 parts of water.Wherein, Chinese medicine is mixed
Object is closed on the basis of its total weight, including 32 parts of Aloses, 15 parts of peppermint powder.Verify the therapeutic effect of Pharmaceutical composition
In 42 nude mice back surgery excisions to form the 1.5cmX1.5cm full thickness skin surface of a wound, nude mice is randomly divided into 7 groups,
Every group 6: first 6 groups are individually smeared the Pharmaceutical composition of 1-6 of the embodiment of the present invention；Last group only smears physiological saline.
At 0,7,14,21,28 day, 1 was put to death respectively.Wound tissue is taken, is fixed with 4% paraformaldehyde, specimens paraffin embedding slices carry out her
LIGNUM SAPPAN uniformly dyeing color, immunohistochemistry and identified by immunofluorescence, carry out the histological examination of wound tissue, and Index for examination is related to wound
Healing time, cicatrization and skin suppleness.
The detection of donor's cells in receptor wound tissue:
A. the identification of green fluorescent protein: in order to study whether human umbilical cord mesenchymal stem cells participate in wound repair, use is green
Color fluorescin plasmid transfected with human umbilical cord mesenchymal stem cells, recipient mice wound cambium is solid with 4% paraformaldehyde after 2 weeks
Fixed, with frozen section embedding medium, -20 DEG C of cryostat microtome slices detect human umbilical cord mesenchymal stem cells with fluorescence microscope
The expression of green fluorescent protein.
B. the detection of cytokeratin and collagen: in order to study whether human umbilical cord mesenchymal stem cells break up in vivo
Epithelial cell forms collagen, and the new raw slicers of Recipient mice do rabbit-anti human keratin (pan-CK) and anti-human 1 Collagen Type VI of rabbit respectively
Protein polyclone antibody is incubated for jointly, is then incubated for the goat anti-rabbit immunoglobulin G (IgG) that fluorescence rhodophyll marks, 0,
7, it is detected with fluorescence microscope within 14,21,28 days.
As it is being listed in following table 1 the results show that 1-6 of the embodiment of the present invention Pharmaceutical composition in human umbilical cord mesenchymal
Stem cell can be excellently attached to wound tissue, and participate in the reparation of epidermis and corium.
The Wound healing and bone regeneration Contrast on effect of table 1. experimental group 1-6 and control group
Described above to be merely exemplary for the purpose of the present invention, and not restrictive, those of ordinary skill in the art understand,
In the case where not departing from spirit and scope as defined in the appended claims, many modifications, variation or equivalent can be made, but all
It will fall within the scope of protection of the present invention.
Claims (10)
1. a kind of Pharmaceutical composition, which is characterized in that the Pharmaceutical composition includes following component: mescenchymal stem cell, Chinese medicine
Mixture；Wherein, the Traditional Chinese drug mixture contains aloe, peppermint；Preferably, using the total weight of described pharmaceutical composition as base
Standard, the mescenchymal stem cell are 5-10 parts, and the Traditional Chinese drug mixture is 15-20 parts；Preferably, with the Traditional Chinese drug mixture
On the basis of total weight, the aloe is 30-60 parts by weight, the peppermint is 10-15 parts by weight.
2. Pharmaceutical composition according to claim 1, wherein the Pharmaceutical composition further includes human serum albumin, low point
One or more of sub- calciparine and vitamin C.
3. Pharmaceutical composition according to claim 2, wherein the Pharmaceutical composition further includes human serum albumin, low point
Sub- calciparine and vitamin C.
4. Pharmaceutical composition according to claim 3, wherein the Pharmaceutical composition further includes ointment, preservative, guarantor
One or more of humectant and water；Preferably, the Pharmaceutical composition further includes ointment, preservative, moisturizer and water.
5. Pharmaceutical composition described in any one of -4 according to claim 1, wherein the Traditional Chinese drug mixture further includes summer cypress
One or more of son, the fleece-flower root, beeswax, Asiatic plantain and dandelion.
6. Pharmaceutical composition according to claim 5, wherein the Traditional Chinese drug mixture further includes the fruit of summer cypress, the fleece-flower root, bee
Wax, Asiatic plantain and dandelion.
7. Pharmaceutical composition described in any one of -4 according to claim 1, wherein the mescenchymal stem cell is between people's umbilical cord
Mesenchymal stem cells.
8. Pharmaceutical composition according to claim 7, wherein the Pharmaceutical composition includes following component: between people's umbilical cord
Mesenchymal stem cells, human serum albumin, Low-molecular-weight Heparins Calcium, vitamin C, Traditional Chinese drug mixture, ointment, preservative, moisturizer and
Water；The Traditional Chinese drug mixture includes aloe, peppermint, the fruit of summer cypress, the fleece-flower root, beeswax, Asiatic plantain, dandelion；Preferably, with described
On the basis of the total weight of pharmaceutical composition, the human umbilical cord mesenchymal stem cells are 5-10 parts, and the human serum albumin is 1-3
Part, the Low-molecular-weight Heparins Calcium is 0.1-0.2 parts, and the vitamin C is 0.3-0.5 parts, and the Traditional Chinese drug mixture is 15-20
Part, the ointment is 20-30 parts, and the preservative is 0.2-0.6 parts, and the moisturizer is 1-1.5 parts, and the water is 86-
93 parts；Wherein, on the basis of the total weight of the Traditional Chinese drug mixture, the aloe is 30-60 parts by weight, the peppermint is 10-
15 parts by weight, the fruit of summer cypress are 8-15 parts by weight, the fleece-flower root is 10-18 parts by weight, the beeswax be 5-10 parts by weight,
The Asiatic plantain is 3-8 parts by weight, the dandelion is 8-15 parts by weight；Preferably, the Pharmaceutical composition is according to following step
Rapid preparation: (1) other compositions except human umbilical cord mesenchymal stem cells in addition to are uniformly mixed；(2) human umbilical cord mesenchymal is dry thin
Born of the same parents are added in the substance of step (1) acquisition.
9. Pharmaceutical composition according to claim 7, wherein the ointment is glycerol；The preservative is selected from sorb
One of acid, butoben and nisin are a variety of；The moisturizer be selected from butanediol, sorbierite and thoroughly
One of bright matter acid sodium is a variety of.
10. the described in any item Pharmaceutical compositions of claim 1-9 are promoting the application in skin wound healing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910215368.2A CN109876012B (en) | 2019-03-21 | 2019-03-21 | Medicinal composition and application thereof in promoting skin wound healing |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910215368.2A CN109876012B (en) | 2019-03-21 | 2019-03-21 | Medicinal composition and application thereof in promoting skin wound healing |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109876012A true CN109876012A (en) | 2019-06-14 |
| CN109876012B CN109876012B (en) | 2022-01-11 |
Family
ID=66933415
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910215368.2A Active CN109876012B (en) | 2019-03-21 | 2019-03-21 | Medicinal composition and application thereof in promoting skin wound healing |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109876012B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110201024A (en) * | 2019-06-26 | 2019-09-06 | 广州南医大生物工程有限公司 | A kind of composition of the extract containing stem cell of wound healing and application thereof |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101287483A (en) * | 2003-08-07 | 2008-10-15 | 希尔洛有限公司 | Pharmaceutical compositions and methods for accelerating wound healing |
| KR20100105138A (en) * | 2009-03-20 | 2010-09-29 | (주)아모레퍼시픽 | Composition for promoting the volume of skin |
| CN101919380A (en) * | 2010-08-06 | 2010-12-22 | 青岛奥克生物开发有限公司 | Improved mesenchyme stem cell protection solution and application thereof |
| CN102228485A (en) * | 2011-06-02 | 2011-11-02 | 叶松 | Composition for burn rehabilitation and preparation method thereof |
| CN102920735A (en) * | 2012-11-14 | 2013-02-13 | 青岛奥克生物开发有限公司 | Mesenchymal stem cell injection, preparation method and application thereof in preparing medicine for treating diabetes |
| CN103494723A (en) * | 2013-06-26 | 2014-01-08 | 马玉贤 | Stem cell skin care composition and manufacturing method and application thereof |
| CN103550117A (en) * | 2013-10-18 | 2014-02-05 | 上海润生生物技术有限公司 | Beauty and skin care product capable of promoting skin repairation and regeneration as well as preparation method and application thereof |
| CN105031622A (en) * | 2015-06-30 | 2015-11-11 | 桂林华诺威基因药业有限公司 | Pharmaceutical composition capable of promoting wound healing, and preparation method thereof |
| CN106237313A (en) * | 2016-09-30 | 2016-12-21 | 广州赛莱拉干细胞科技股份有限公司 | A kind of umbilical cord mesenchymal stem cells injection and its preparation method and application |
| US20180296631A1 (en) * | 2017-04-12 | 2018-10-18 | University Of Toronto | Hydrogel composition and associated method of use |
-
2019
- 2019-03-21 CN CN201910215368.2A patent/CN109876012B/en active Active
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101287483A (en) * | 2003-08-07 | 2008-10-15 | 希尔洛有限公司 | Pharmaceutical compositions and methods for accelerating wound healing |
| CN102120031A (en) * | 2003-08-07 | 2011-07-13 | 希尔洛有限公司 | Pharmaceutical compositions and methods for accelerating wound healing |
| KR20100105138A (en) * | 2009-03-20 | 2010-09-29 | (주)아모레퍼시픽 | Composition for promoting the volume of skin |
| CN101919380A (en) * | 2010-08-06 | 2010-12-22 | 青岛奥克生物开发有限公司 | Improved mesenchyme stem cell protection solution and application thereof |
| CN102228485A (en) * | 2011-06-02 | 2011-11-02 | 叶松 | Composition for burn rehabilitation and preparation method thereof |
| CN102920735A (en) * | 2012-11-14 | 2013-02-13 | 青岛奥克生物开发有限公司 | Mesenchymal stem cell injection, preparation method and application thereof in preparing medicine for treating diabetes |
| CN103494723A (en) * | 2013-06-26 | 2014-01-08 | 马玉贤 | Stem cell skin care composition and manufacturing method and application thereof |
| CN103550117A (en) * | 2013-10-18 | 2014-02-05 | 上海润生生物技术有限公司 | Beauty and skin care product capable of promoting skin repairation and regeneration as well as preparation method and application thereof |
| CN105031622A (en) * | 2015-06-30 | 2015-11-11 | 桂林华诺威基因药业有限公司 | Pharmaceutical composition capable of promoting wound healing, and preparation method thereof |
| CN106237313A (en) * | 2016-09-30 | 2016-12-21 | 广州赛莱拉干细胞科技股份有限公司 | A kind of umbilical cord mesenchymal stem cells injection and its preparation method and application |
| US20180296631A1 (en) * | 2017-04-12 | 2018-10-18 | University Of Toronto | Hydrogel composition and associated method of use |
Non-Patent Citations (4)
| Title |
|---|
| 徐书: "《杏林碎金录 30年皮外科秘典真传》", 28 February 2017, 中国科学技术出版社 * |
| 李军德: "《常用动物药材识别图鉴》", 31 March 2017, 福建科学技术出版社 * |
| 陈明岭等: "《皮肤病常用中药药理及临床》", 31 October 2017 * |
| 高学敏: "《中华医学百科全书 中药学》", 30 June 2017, 中国协和医科大学出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110201024A (en) * | 2019-06-26 | 2019-09-06 | 广州南医大生物工程有限公司 | A kind of composition of the extract containing stem cell of wound healing and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109876012B (en) | 2022-01-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Oh et al. | Activated platelet-rich plasma improves fat graft survival in nude mice: a pilot study | |
| Asboe-Hansen | Hormonal effects on connective tissue | |
| Thakur et al. | Practices in wound healing studies of plants | |
| Khorasani et al. | The effect of saffron (Crocus sativus) extract for healing of second-degree burn wounds in rats | |
| CN103096900B (en) | Anti-oxidant compositions | |
| AU599647B2 (en) | Wound healing composition | |
| WO2021103581A1 (en) | Skin topical composition having efficacy of promoting wound healing and/or scar repair | |
| CN109674686A (en) | A kind of composition and preparation method thereof of maintenance damaged skin | |
| WO2024045952A1 (en) | Recombinant collagen-containing composition having effects of repairing and soothing, eye cream containing same, preparation method therefor, and use thereof | |
| CN109453090A (en) | A kind of moisturizing whitening, the Essence composition of compact promotion and preparation method thereof | |
| CN109675085A (en) | A kind of composite collagen dressing and preparation method thereof for burn wound reparation | |
| WO2021143096A1 (en) | Whitening, acne-removing, blackhead-removing and anti-aging composition, and use and preparation thereof | |
| Vasconcelos et al. | A phytomodulatory hydrogel with enhanced healing effects | |
| CN108703931A (en) | A kind of composition and preparation method thereof with moisture-keeping function | |
| Han et al. | Combining platelet-rich plasma and tissue-engineered skin in the treatment of large skin wound | |
| CN102908366A (en) | Preparation method of chick embryo active component | |
| CN109876012A (en) | A kind of Pharmaceutical composition and its application in promotion skin wound healing | |
| CN101810704A (en) | Combination of traditional Chinese medicine extractive with function of removing acne, preparation and use | |
| CN110327412B (en) | A tea oil-Aloe cream and its preparation method | |
| Ma et al. | Walnut oil promotes healing of wounds and skin defects in rats via regulating the NF-kB pathway | |
| Bazafkan et al. | Wound healing effect of an ointment made from a mixture of brassica oleracea var, Punica granatum, and Plantago major L Extracts in Rats | |
| CN106729125B (en) | Composition for treating acne and application thereof | |
| Ugwudike Patrick et al. | Python fat: effect on collagen levels of human keloid tissue. | |
| CN112107633B (en) | Compound zanthoxylum bungeanum gel and preparation method thereof | |
| CN109833339B (en) | Composition for promoting proliferation of stem cells and tissue regeneration of skin injury wound |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |