KR20140097095A - Composition for promoting the volume of skin - Google Patents

Abstract

The present invention relates to a composition for promoting the differentiation of human mesenchymal stem cells into adipocytes. More specifically, the present invention relates to a composition for cosmetics or plastic surgery which increases the sense of volume of specific body part by creating lipid droplets by comprising at least one species among Castaneae semen, Selaginella tamariscina, Aloe vera, Salvia miltiorrhiza, Mentha arvensis, Pinellia ternate, Triticum aestivum, Saururus chinensis, Polygala tenuifolia, and Gleditsia sinensis extracts as an active ingredient to promote the differentiation of human mesenchymal stem cells into adipocytes.

Description

TECHNICAL FIELD The present invention relates to a composition for promoting volume increase of skin,

The present invention relates to a composition for promoting the differentiation of human mesenchymal stem cells into adipocytes. More particularly, the present invention relates to a composition for promoting the differentiation of human mesenchymal stem cells into adipocytes, As an active ingredient to promote the differentiation of human mesenchymal stem cells into adipocytes, thereby producing a lipid droplet, thereby increasing the volume of the skin of a specific site.

Now that the S line is becoming popular, the demands of modern women who are trying to make a good body shape are increasing. In particular, the soft lines that lead to the chest, waist and butt reveal feminine charm, and the tough, voluminous buttocks are enough to keep the dressing up when wearing pants. In addition to young women, the decrease in fat due to metabolic abnormalities such as hormones and blood circulation, which are mainly caused by menopausal women, causes women to feel a great loss of femininity. As such, increasing the volume of local organizations is becoming a solution to problems that are very sensitive to modern-day women, and many women are trying to maintain or increase the volume of skin through plastic surgery with side effects and economic burdens. do.

In order to improve the volume feeling of such skin, a currently widely used method is a fat transplant operation in which the user transplants his or her own fat to each part of the skin required. These fat grafts have shown good results by applying lip line, chin line, correction of forehead line, nose formation, depressed skin due to burns or wounds, and areas lost by cancer resection surgery. However, it has been reported that 40 to 60% of the transplant volume is resorbed in the human body after fat transplant surgery (Eremia S et al ., Dermatol . Sur . 2000, 26: 1150-1158; Fulton JE et al ., Dermatol . Clin . 2001, 19 (3): 523-530). However, fat transplant surgery in areas where skin is thin and subcutaneous fat is low causes problems such as cell clusters being touched and the survival rate and survival time of transplanted fat The re-implantation is necessary. Accordingly, there is a growing demand for a composition capable of sufficiently increasing the subcutaneous fat layer while replacing the fat grafting surgery with these problems.

According to recent research results, the basic structure of the skin is maintained by subcutaneous fat tissue, and since the subcutaneous fat tissue plays a role in the volume and intensity of the skin, (Trottier V et et al. al ., Stem Cells . 2008 26 (10): 2713-23; Park BS et al ., Dermatol . Surg . 2008, 34 (10): 1323-1326). Adipocytes form a subcutaneous fat layer under the skin's dermis and serve as a cushion to absorb the protection of the internal organs of the skin against external heat and shocks, and the nutrients in the form of triglycerides It is stored in a cell organelle called Lipid droplet and is used as an energy source when necessary.

Studies using these adipocytes have been actively conducted in various fields. Generally used preadipocytes are widely used because they have the property of differentiating into adipocytes by differentiation inducers such as insulin. In particular, mesenchymal stem cells and adipocyte derived stem cells have recently attracted attention in research using adipocytes. Since these stem cells can be differentiated using methods similar to those used to differentiate adipocyte precursor cells into adipocytes (Xu Y et al ., Exp. Cell Res 2006, 312, 1856-1864) , And is widely used in studies of adipocyte differentiation (Pittenger MF et al ., Science 1999, 284, 143-146).

Therefore, the present inventors have made efforts to find out the herbal medicine extract promoting the differentiation of adipocytes constituting adipose tissue. As a result, the present inventors have found that the extracts of Sungkyul, Kwak, The inventors of the present invention discovered that mesenchymal stem cells differentiate into adipocytes effectively, and completed the present invention.

Accordingly, an object of the present invention is to provide a cosmetic or molding composition which contains a herbal medicine extract promoting the differentiation of human mesenchymal stem cells into adipocytes, thereby increasing the volume feeling of the skin.

In order to achieve the above object, the present invention provides a method for promoting differentiation of adipocytes, which comprises, as an active ingredient, one or more extracts selected from the group consisting of dry weight, pulling gum, presbyopia, danshen, mint, An external application composition and a pharmaceutical composition are provided.

The composition according to the present invention contains at least one kind of dry matter extract, extract, sperm, sperm, dansamp, mint, bangha, wheat, sorghum, raw paper and sculpture extract as active ingredients to promote differentiation of mesenchymal stem cells into adipocytes It is useful for increasing the adipose tissue for cosmetic or cosmetic purposes and thus helps to increase skin volume.

FIG. 1 is a photograph showing microscopic observation of the adipocyte stem cells after induction of differentiation of mesenchymal stem cells under IDXT conditions and IDXT treatment under the IDX condition.
FIGS. 2A to 2H are graphs showing relative lipid differentiation values of IDFs of mesenchymal stem cells after treatment with dry matter, spinach, presbyoptera, danshen, mint, bangha, wheat,

The present invention provides a composition for promoting differentiation of adipocytes, which comprises, as an active ingredient, at least one extract selected from the group consisting of dry weight, pomace, presbyopia, dansamp, mint, bamboo, wheat,

As used herein, the term " adipocyte differentiation " refers to the differentiation of adipose precursor cells or mesenchymal stem cells of an animal, preferably a mammal, into adipocytes, more preferably human mesenchymal stem cells, Is the differentiation of mesenchymal stem cells derived from human bone marrow into adipocytes.

Herbal medicine extracts used in the present invention are as follows.

Castaneae Semen has a characteristic odor, flavor and warmth. The effect is the effect of the base, stop the diarrhea and enhance the renal function is used for backache and leg weakness. It is also effective in hemostatic and bronchitis. Antioxidant activity has been reported as a pharmacological effect, and it is reported that vitamin C is abundant, which is effective for skin beauty, fatigue recovery and cold prevention.

Selaginella tamariscina is odorless, has a very high taste, and its properties are flat without any bias. The effect is hemorrhage in various hemorrhages, and it is used for hemorrhagic bleeding, hematemesis, urogenital hemorrhage and uterine hemorrhage. The raw hemorrhoids are effective for blood circulation and are used for menstrual cramps, bruises, and bruises. Staphylococcus aureus inhibition has been reported as a pharmacological action.

Presbytery (Aloe vera L.) refers to one species or all of the plants belonging to the genus Aloe (Aloe). Africa is a country of origin, there are about 300 species worldwide. It grows in the greenhouse as a houseplant or grows at home to use as medicine. It has germicidal properties against bacteria and fungi, contains aloe tin which neutralizes toxins, aloe vera effective for ulcers and alomitin for anti-cancer effects. Other ingredients include steroids, amino acids, saponins, antibiotics, wound healing hormones and minerals. Aloe is effective in relieving hangovers due to overwork and restoring fatigue. If yellow leaves of aloe are cut off, a yellowish substance flows out, which is especially effective for constipation. In the private sector, aloe leaves juice is used for gastrointestinal diseases and for trauma and burns. It also neutralizes dry and oily skin, and is also used as a cosmetic ingredient because it has a moisturizing effect on the skin.

Mentha I have arvensis . piperascens Makinv.) has a peculiar smell and if you put it in your mouth it will be cool if you give it a cool feeling. Efficacy is used for symptoms of fever, headache and sweating caused by colds of the eyes, eye congestion, sore throat, early measles itching, liver disease, abdominal pain, diarrhea and vomiting. Pharmacological actions have been reported such as fever, inflammation, dryness, promotion of bile secretion and gastrointestinal smooth muscle suppression, increase of mucus secretion of the respiratory tract, central nervous excitement, and uterine contraction.

Half summer ( Pinellia ternata (Thunb.) Breit.) has little odor and chews a little mucus, but later on it is very brittle, spicy, temperate and poisonous. It is used for sputum, seawater and asthma because it treats dampness. It is used for headache due to wall, dizziness, chest complaints, symptoms, vomiting, sore throat, swelling on the back, mastitis, pregnancy and vomiting. Pharmacological actions such as genome, Jinhae, vomiting, prevention of silicosis, anticancer effects were reported.

Wheat ( Triticum ) aestivum L.) is also called wheat. Wheat is used as an auxiliary food in the Orient, but it is the main food in the West and is the world's second largest food crop with rice. More than 90% are milled for noodles, baking, confectionery, and industrial use. It is also used as a raw material for soy sauce and miso, and bran is a good feed. Straws are used to make straw hats and so on.

Saururus chinensis Baill.) is a fish that smears when it rubs the leaves, it is spicy and its taste is a bit cold. The effect is excellent in fever and drainage function. It is used for coughing caused by lung abscess, vomiting, pneumonia, acute chronic bronchitis, enteritis, urinary tract infections, boils, and it is used in case of fever and urine. Antimicrobial activity, immune enhancement, antiinflammatory, diuretic and vasodilating effects were reported as pharmacological actions.

Polygala tenuifolia Willd.) is thick and long roots, and a few stems come out from the end in a bunch and have little hair. Leaves are alternate phyllotaxis, 1.5-3cm long and have no petiole. The petal has an upper part, a lower part, and a tip that divides finely like a brush. Fruit is capillary (果), flat and divided into two, and seeds are dense with hair. In one room, it is called roots, and it is written as expository, tangible, and kangjeongje. It is distributed in the north of central Korea and northern part of China.

Sculpture ( Gleditsia sinensis Lam.) Is a medicinal herb that is used in various folk remedies such as improving bowel function in East Asia. The thorns are called sculptures, and they have efficacy such as excretion, drainage and so on. Winter is also possible in the central region of Korea.

丹 蔘 ( Salvia miltiorrhiza Bunge.) Is also called red ginseng (red ginseng), with a somewhat unusual aroma, weakness is worn and slightly cold. The effect helps blood circulation, eliminates eosinophilia, and alleviates pain in the extremities. Men's physiological impoverishment, menstrual cramps, postpartum abdominal pain, abdominal fullness of the abdomen, bruises, etc. are treated. It is used to treat mental confusion, bullshit, fury, insomnia, skin rash, palpitations, etc. due to high fever. Pharmacological actions include coronary artery dilation, cholesterol depression, hypotension, liver function activation, sedative anti-inflammatory, anti-cancer, and anti-bacterial effects.

The extracts obtained by extracting dry matter such as dry matter, spinach, spinach, danshen, mint, bananas, wheat, sorghum, raw paper and sculpture, which are used in the present invention, with an organic solvent such as water or ethanol by conventional extraction methods well known in the art .

The composition of the present invention may be provided as a composition for external application for skin or a pharmaceutical composition, which promotes differentiation of mesenchymal stem cells (MSC), thereby forming subcutaneous fat layers or providing fat cells to give a volume feeling to the skin have.

When the composition of the present invention is used as a composition for external application for skin, it is not particularly limited in its formulation depending on the body part. Specifically, it may be a cosmetic composition having a formulation of a softening agent, a nutritional lotion, a massage cream, a nutritional cream, a pack, a gel or a skin adhesive type cosmetic composition and may be a cosmetic composition such as lotion, ointment, gel, cream, patch or spray It may be a transdermal dosage form.

The composition for external application for skin according to the present invention is characterized by comprising at least one extract selected from the group consisting of dry weight, In an amount of 30% by weight.

In the composition for external application for skin according to each of the formulations, components other than the above-mentioned extract of dry matter of the present invention, dry matter of the present invention, presbyopia, presbyoptera, mint, mint, And the like, it is possible for a person skilled in the art to mix and choose without any difficulty. In this case, a synergistic effect may occur when applied to other raw materials at the same time.

In addition, when the composition according to the present invention is used as a pharmaceutical composition, a pharmaceutical adjuvant such as a preservative, a stabilizer, a wetting agent or an emulsifying accelerator, a salt for controlling osmotic pressure and / or a buffer, And may be formulated into various oral or parenteral dosage forms according to conventional methods.

Examples of formulations for oral administration include tablets, pills, hard and soft capsules, liquids, suspensions, emulsions, syrups, granules, etc. These formulations may contain, in addition to the active ingredient, a diluent such as lactose, dextrose, Mannitol, sorbitol, cellulose and glycine) and lubricants (such as silica, talc, stearic acid and its magnesium or calcium salts and polyethylene glycols). The tablets may also contain binders such as magnesium aluminum silicate, starch paste, gelatin, tragacanth, methylcellulose, sodium carboxymethylcellulose and polyvinylpyrrolidine, optionally mixed with starch, agar, alginic acid or its sodium salt Such as disintegrants, absorbents, coloring agents, flavoring agents, and sweetening agents. Tablets can be prepared by conventional mixing, granulating or coating methods. Also typical of parenteral administration formulations are isotonic aqueous solutions or suspensions which are suitable for injectable use. It should be understood, however, that the actual dosage of the active ingredient should be determined in light of various relevant factors such as the severity of the symptoms, the route of administration selected, the age, sex, weight and health of the subject, Are not intended to limit the scope of the invention in any way. In general, the dosage of herbal extracts is in the range of 0.001 mg / kg / day to about 2000 mg / kg / day. A more preferred dosage is 0.5 mg / kg / day to 2.5 mg / kg / day.

The application site of the cosmetic or molding composition for promoting differentiation of mesenchymal stem cells according to the present invention includes all the adipose tissues of the body and is not limited to a specific site. For example, a breast, a hip Region. The effect of increasing the fat tissue includes, but is not limited to, an effect of increasing fat tissue for cosmetic or cosmetic purposes.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the following examples are intended to illustrate the present invention, but the scope of the present invention is not limited thereto.

[Example 1] Preparation of herbal medicine extract

(95.0 ~ 95.5v / v%) of 30g of each of the dried, dried, dried, dried, dried, ), And then pulverized for 20 minutes under a pressure of 1500 psi at 50 DEG C using an ASE300 ACCELERATED SOLVENT EXTRACOR (DIONEX Corporation) apparatus. Further, it was further pulverized at 50 DEG C for 3 days using an ultrasonic washing machine (BRANSON Ultrasonics corporation). Each of the thus-pulverized extracts was dried at 40 ° C for 24 hours using a Modul spin 40 (Biotron corporation) apparatus to prepare samples of each extract.

[Test Example 1] Efficacy and quantitative measurement of promoting differentiation of human bone marrow-derived mesenchymal stem cells into adipocytes

Human bone marrow mesenchymal stem cells (hBM-MSCs) were purchased from Lonza, Inc. (Walkersville, MD, USA) and cultured according to Lonza's guidelines. Adipocyte differentiation was achieved using the method recommended by Ron, except that troglitazone was used instead of indomethacin to induce adipocyte differentiation of mesenchymal stem cells. Specifically, mesenchymal stem cells were cultured in the presence of insulin 1 μg / ml, dexamethasone (DEXA) 1 μM, isobutyl methyl xanthine (IBMX) 0.5 mM, and the stem cells were differentiated into adipocytes And 10 ppm of extracts were added to the medium under the IDXT condition at the induction of adipocyte differentiation, respectively. For comparison, 10 ppm of each of male, white paper, phloem, chrysanthemum, juvenile, mussel, amaranth, or pancreatic extract was added to the medium. As a positive control, troglitazone (TRO) 2 μM was used and treated with IDX medium. After 14 days from the start of adipocyte differentiation of mesenchymal stem cells, the cell culture medium was removed, and the cells were washed with 10 ml of phosphate buffered saline (PBS). Then, 0.2 ml / cm 2 of 10% formalin / PBS solution ² was used to fix the cells for 10 minutes. The fixed cells were washed once with a 60% isopropanol solution (0.5 ml / cm ² ), and the lipid in the adipocyte was stained with 0.2 ml / cm ² of a dye solution obtained by dissolving Oil Red O in 60% isopropanol Respectively. Subsequently, re-staining with hematoxylin was performed to easily distinguish the nucleus and then observed with a microscope. When the extract was treated with dry matter, Kwonbyeong, presbyoptera, dansam, mint, bangha, wheat, The results are shown in Fig.

FIG. 1 shows that, in the case of inducing adipocyte differentiation of mesenchymal stem cells after addition of the extract of Root extract under IDXT condition in comparison with the induction of adipocyte differentiation of mesenchymal stem cells under IDX or IDXT conditions, Can be found more effectively.

Then, the dyed oil red oi was eluted with a 100% isopropanol solution, and the absorbance was measured at a wavelength of 500 nm. The results are shown in Figs. 2A to 2H. At this time, each data value was corrected with an insulin control group. These increases were statistically significant, and statistical tests were verified by both Student's t test, indicating a significant difference when the p-value was less than 0.05. In addition, IDXT of mesenchymal stem cells when treated with extracts of dry matter, Kwonbaek, presbytery, Dansam, mint, Banha, wheat, And the rate of increase in the promotion of fat differentiation was 30% or more, as shown in Table 1

A good name Scientific name IDXT contrast (growth rate) Dry rate Castaneae Semen 30% The Selaginella tamariscina 40% craftiness Aloe 47% Sculpture Gleditsiae Semen 31% Sansam Salviae Radix 38% Mint (Sat) Menthae Herba 33% Inferiority Pinelliae Tuber 38% Wheat Tritici Semen 31% Origin Polygalae Radix 37% Horseshoe (Chinese medicine) Houttuyniae Herba 34% male Arisaematis Rhizoma 0.017% blank Angelicae Dahuricae Radix 6% reduction Bae Hoon Pulsatillae Radix 19% Moonlight Pini Ramulus 25% Cerebellum Borneolum 0.08% The Achuranthis Radix 0.1% Flax Lini Semen 3.5% reduction Atmosphere Benzoinum 21%

As can be seen from the results of Table 1 and Figs. 2 (a) to 2 (h), the extracts of the dry matter of the present invention exhibited the cell death of mesenchymal stem cells And promotes the differentiation of adipocyte cells without inducing apoptosis and promotes the differentiation of adipocyte cells more effectively than in the case of treatment with male, white papillomous, phloem, pineal gland, juvenile, .

[Test Example 2] Measuring effect of enhancing skin volume

In order to investigate the effect of the extract of the present invention on the volume of the human skin when using the dry matter extract, the extract of Kwang Baek, the presbyoptera, the dansamp, the mint, the peach, the wheat, .

Specifically, a nutritional cream containing 2.5% by weight of each of the above extracts as shown in Table 2 was applied to the faceball area for 8 weeks once a day for one week. After that, Respectively. When the volume enhancing effect is good, it is indicated by o, when it is normal, when it is not, and when it is not, it is indicated by x, and the results are shown in Table 3 below.

Compounding ingredient Content (% by weight) Purified water To 100 Dry matter, extracts, presbyopia, danshang, mint, bamboo, wheat, sorghum, 2.5 Vegetable hydrogenated oil 1.5 Stearic acid 0.6 Glycerol stearate 1.0 Cetearyl alcohol 2.0 Polyglyceryl-10pentastearate and behenyl alcohol and sodium stearoyl lactylate 1.0 Arachidyl behenyl alcohol 1.0 Cetylaryl alcohol & cetearyl glucoside 2.0 PEG-100 Stearate & Glycerololate & Propylene Glycol 1.5 Caprylic / Carrick Triglyceride 11.0 Cyclomedicone 6.0 Preservative, incense Suitable amount Triethanolamine 0.1

A good name Volume enhancement effect (persons) ○ △ × Dry rate 10 2 3 The 9 4 2 craftiness 11 2 2 Sansam 8 4 3 mint 8 5 2 Inferiority 12 2 One Wheat 9 2 4 Sophora 10 3 2 Origin 12 One 2 Sculpture 9 3 3 blank 2 4 9

As can be seen from the above Table 3, in the case of using the white paper extract, the effect of enhancing the skin volumizing feeling is not good, while the extract of the present invention, , It can be confirmed that the skin volumizing effect is good for most test participants.

From the results of the above experiment, it was found that when the compositions containing the extracts of the dry matter, the pine needle, the presbyopia, the dansamp, the mint, the pineapple, the wheat, the pine needle, the raw paper or the sculpture were respectively applied to the facial skin, The volume of the tissue is increased, and thus the effect of increasing the volume and elasticity of the skin can be obtained.

Examples of formulations for the composition of the present invention are illustrated below.

[Formulation Example 1] Tablets

100 mg of the extract of one kind among the dry matter of the present invention, one or two kinds of the extracts of calamus, licorice, presbyoptera, dansamp, mint, bamboo, wheat, sorghum, ground and sculpture was mixed with 400 mg of lactose, 400 mg of corn starch and 2 mg of magnesium stearate, Tablets were prepared by tabletting according to the manufacturing method.

[Formulation Example 2]

After mixing 100 mg of extract of one kind among dry matter, paulownia seeds, presbyoptera, danshang, mint, bamboo, wheat, hornblende, raw paper and sculpture, 400 mg of lactose, 400 mg of corn starch and 2 mg of magnesium stearate, Thus, capsules were prepared by filling in gelatin capsules.

[Formulation Example 3] Softening lotion (skin lotion)

Compounding ingredient Content (% by weight) Purified water To 100 Extract of one kind of dry matter, Kwakbaek, presbytery, Dansam, mint, peel, wheat, 0.1 to 0.2 Butylene glycol 2.0 Propylene glycol 2.0 Carboxyvinyl polymer 0.1 Phage-12 nonylphenyl ether 0.2 Polysorbate 80 0.4 ethanol 10.0 Triethanolamine 0.1 Preservative, coloring, fragrance Suitable amount

[Formulation Example 4] Nutritional lotion (milk lotion)

Compounding ingredient Content (% by weight) Purified water To 100 Extract of one kind of dry matter, Kwakbaek, presbytery, Dansam, mint, peel, wheat, 0.1 to 0.2 Wax 4.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.5 Liquid paraffin 0.5 Caprylic / capric triglyceride 5.0 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxyvinyl polymer 0.1 Triethanolamine 0.2 Preservative, coloring, fragrance Suitable amount

[Formulation Example 5] Nourishing cream

Compounding ingredient Content (% by weight) Purified water To 100 Extract of one kind of dry matter, Kwakbaek, presbytery, Dansam, mint, peel, wheat, 0.1 to 0.2 Wax 10.0 Polysorbate 60 1.5 Piggy 60 hardened castor oil 2.0 Sorbitan sesquioleate 0.5 Liquid paraffin 10.0 Squalane 5.0 Caprylic / capric triglyceride 5.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 Preservative, coloring, fragrance Suitable amount

[Formulation Example 6] Massage cream

Compounding ingredient Content (% by weight) Purified water To 100 Extract of one kind of dry matter, Kwakbaek, presbytery, Dansam, mint, peel, wheat, 0.1 to 0.2 Wax 10.0 Polysorbate 60 1.5 Piggy 60 hardened castor oil 2.0 Sorbitan sesquioleate 0.8 Liquid paraffin 40.0 Squalane 5.0 Caprylic / capric triglyceride 4.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 Preservative, coloring, fragrance Suitable amount

[Formulation Example 7] Pack

Compounding ingredient Content (% by weight) Purified water To 100 Extract of one kind of dry matter, Kwakbaek, presbytery, Dansam, mint, peel, wheat, 0.1 to 0.2 Polyvinyl alcohol 13.0 Sodium carboxymethylcellulose 0.2 glycerin 5.0 Allantoin 0.1 ethanol 6.0 Phage-12 nonylphenyl ether 0.3 Polysorbate 60 0.3 Preservative, coloring, fragrance Suitable amount

Claims (9)

A composition for external application for skin, which comprises, as an active ingredient, at least one extract selected from the group consisting of Phellinus linteus, presbyoptera, mint, peach, wheat, sorghum, raw paper and sculpture. The composition for external application for skin according to claim 1, wherein the composition promotes the differentiation of mesenchymal stem cells into adipocytes. 3. The composition for external application for skin according to claim 2, wherein the mesenchymal stem cells are human mesenchymal stem cells. The composition for external application for skin according to claim 2, wherein the composition increases fat tissue. [3] The composition according to claim 1, wherein the composition contains at least one extract from at least one selected from the group consisting of pomegranate, presbyoptera, mint, bamboo, wheat, sorghum, ground and sculptured in an amount of 0.0001 to 30% by weight based on the total weight of the composition Wherein the external preparation is a skin external preparation. The composition for external application for skin according to claim 1, wherein the composition increases skin volume. A pharmaceutical composition for promoting the differentiation of adipocytes, which comprises, as an active ingredient, at least one extract selected from the group consisting of Phellinus linteus, presbyoptera, mint, alfalfa, wheat, The pharmaceutical composition according to claim 7, wherein the composition promotes the differentiation of mesenchymal stem cells into adipocytes. [Claim 7] The composition according to claim 7, wherein the composition has a dosage of 0.001 mg / kg / day to 2000 mg / kg / day of one or more extracts selected from the group consisting of Ganoderma lucidum, ≪ / RTI >

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