KR20100101689A - 항생제-없는 ＣｏｌＥ１ 플라스미드 증식을 위한 숙주-벡터 시스템 - Google Patents
항생제-없는 ＣｏｌＥ１ 플라스미드 증식을 위한 숙주-벡터 시스템 Download PDFInfo
- Publication number
- KR20100101689A KR20100101689A KR1020107017286A KR20107017286A KR20100101689A KR 20100101689 A KR20100101689 A KR 20100101689A KR 1020107017286 A KR1020107017286 A KR 1020107017286A KR 20107017286 A KR20107017286 A KR 20107017286A KR 20100101689 A KR20100101689 A KR 20100101689A
- Authority
- KR
- South Korea
- Prior art keywords
- rna
- plasmid
- gene
- sequence
- dna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- 239000013612 plasmid Substances 0.000 title claims abstract description 150
- 239000013598 vector Substances 0.000 title abstract description 20
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 205
- 239000003550 marker Substances 0.000 claims abstract description 75
- 230000014509 gene expression Effects 0.000 claims abstract description 69
- 102000004169 proteins and genes Human genes 0.000 claims description 58
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 26
- 230000001580 bacterial effect Effects 0.000 claims description 25
- 230000010076 replication Effects 0.000 claims description 25
- 230000001225 therapeutic effect Effects 0.000 claims description 6
- 108020005091 Replication Origin Proteins 0.000 claims description 3
- 241000124008 Mammalia Species 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 17
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 abstract description 8
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 abstract description 8
- 230000008844 regulatory mechanism Effects 0.000 abstract 1
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 77
- 108020004414 DNA Proteins 0.000 description 73
- 108091030071 RNAI Proteins 0.000 description 68
- 239000005090 green fluorescent protein Substances 0.000 description 51
- 102000004144 Green Fluorescent Proteins Human genes 0.000 description 50
- 108010043121 Green Fluorescent Proteins Proteins 0.000 description 50
- 210000004027 cell Anatomy 0.000 description 46
- 231100000331 toxic Toxicity 0.000 description 34
- 230000002588 toxic effect Effects 0.000 description 34
- 238000013518 transcription Methods 0.000 description 29
- 230000035897 transcription Effects 0.000 description 29
- 108700039887 Essential Genes Proteins 0.000 description 26
- 230000001105 regulatory effect Effects 0.000 description 24
- 108020004999 messenger RNA Proteins 0.000 description 22
- 238000002474 experimental method Methods 0.000 description 21
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 20
- 230000001939 inductive effect Effects 0.000 description 19
- 238000000034 method Methods 0.000 description 19
- 241000588724 Escherichia coli Species 0.000 description 17
- 238000000855 fermentation Methods 0.000 description 17
- 230000004151 fermentation Effects 0.000 description 17
- 230000014616 translation Effects 0.000 description 14
- 238000013519 translation Methods 0.000 description 14
- 210000000349 chromosome Anatomy 0.000 description 13
- 230000012010 growth Effects 0.000 description 13
- 230000003993 interaction Effects 0.000 description 13
- 231100000518 lethal Toxicity 0.000 description 13
- 230000001665 lethal effect Effects 0.000 description 13
- 230000000295 complement effect Effects 0.000 description 12
- 108091034117 Oligonucleotide Proteins 0.000 description 11
- 239000012634 fragment Substances 0.000 description 11
- 230000006698 induction Effects 0.000 description 11
- 230000005764 inhibitory process Effects 0.000 description 11
- 239000002609 medium Substances 0.000 description 11
- 238000012360 testing method Methods 0.000 description 11
- 230000000694 effects Effects 0.000 description 10
- 238000001415 gene therapy Methods 0.000 description 10
- 101150023205 murA1 gene Proteins 0.000 description 10
- 101150089003 murA2 gene Proteins 0.000 description 10
- 238000011144 upstream manufacturing Methods 0.000 description 10
- 101100239088 Bacillus subtilis (strain 168) murAA gene Proteins 0.000 description 9
- 101100131847 Bacillus subtilis (strain 168) murAB gene Proteins 0.000 description 9
- 230000010261 cell growth Effects 0.000 description 9
- 230000003828 downregulation Effects 0.000 description 9
- 239000000411 inducer Substances 0.000 description 9
- 230000002401 inhibitory effect Effects 0.000 description 9
- 101150025333 murA gene Proteins 0.000 description 9
- 108091081024 Start codon Proteins 0.000 description 8
- 238000000338 in vitro Methods 0.000 description 8
- 229930027917 kanamycin Natural products 0.000 description 8
- 229960000318 kanamycin Drugs 0.000 description 8
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 8
- 229930182823 kanamycin A Natural products 0.000 description 8
- 230000004044 response Effects 0.000 description 8
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 7
- 239000003242 anti bacterial agent Substances 0.000 description 7
- 230000000692 anti-sense effect Effects 0.000 description 7
- 230000003115 biocidal effect Effects 0.000 description 7
- 230000006870 function Effects 0.000 description 7
- 239000013642 negative control Substances 0.000 description 7
- 241000894006 Bacteria Species 0.000 description 6
- 108700008625 Reporter Genes Proteins 0.000 description 6
- 229940088710 antibiotic agent Drugs 0.000 description 6
- 238000013459 approach Methods 0.000 description 6
- 230000033228 biological regulation Effects 0.000 description 6
- 230000015572 biosynthetic process Effects 0.000 description 6
- 239000001963 growth medium Substances 0.000 description 6
- 230000007246 mechanism Effects 0.000 description 6
- 230000001404 mediated effect Effects 0.000 description 6
- 239000000758 substrate Substances 0.000 description 6
- 230000001018 virulence Effects 0.000 description 6
- 239000004098 Tetracycline Substances 0.000 description 5
- 210000003578 bacterial chromosome Anatomy 0.000 description 5
- 238000010367 cloning Methods 0.000 description 5
- 239000003184 complementary RNA Substances 0.000 description 5
- 238000009396 hybridization Methods 0.000 description 5
- 230000001965 increasing effect Effects 0.000 description 5
- 239000002773 nucleotide Substances 0.000 description 5
- 125000003729 nucleotide group Chemical group 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- 229960002180 tetracycline Drugs 0.000 description 5
- 229930101283 tetracycline Natural products 0.000 description 5
- 235000019364 tetracycline Nutrition 0.000 description 5
- 150000003522 tetracyclines Chemical class 0.000 description 5
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 4
- 238000012258 culturing Methods 0.000 description 4
- 238000001727 in vivo Methods 0.000 description 4
- 238000003780 insertion Methods 0.000 description 4
- 230000037431 insertion Effects 0.000 description 4
- 230000010354 integration Effects 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- 101150061166 tetR gene Proteins 0.000 description 4
- 108700020534 tetracycline resistance-encoding transposon repressor Proteins 0.000 description 4
- 108020005544 Antisense RNA Proteins 0.000 description 3
- 244000063299 Bacillus subtilis Species 0.000 description 3
- 235000014469 Bacillus subtilis Nutrition 0.000 description 3
- 108010046276 FLP recombinase Proteins 0.000 description 3
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 3
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 3
- 108010090804 Streptavidin Proteins 0.000 description 3
- 101100309436 Streptococcus mutans serotype c (strain ATCC 700610 / UA159) ftf gene Proteins 0.000 description 3
- 229930006000 Sucrose Natural products 0.000 description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 3
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 3
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 description 3
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 239000013604 expression vector Substances 0.000 description 3
- 230000004927 fusion Effects 0.000 description 3
- 230000002068 genetic effect Effects 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 238000002372 labelling Methods 0.000 description 3
- 239000008101 lactose Substances 0.000 description 3
- 230000006798 recombination Effects 0.000 description 3
- 238000005215 recombination Methods 0.000 description 3
- 230000002829 reductive effect Effects 0.000 description 3
- 101150025220 sacB gene Proteins 0.000 description 3
- 239000005720 sucrose Substances 0.000 description 3
- 238000010361 transduction Methods 0.000 description 3
- 230000026683 transduction Effects 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 108700003860 Bacterial Genes Proteins 0.000 description 2
- 241000252203 Clupea harengus Species 0.000 description 2
- 108020004394 Complementary RNA Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 2
- 101710163270 Nuclease Proteins 0.000 description 2
- 108010034634 Repressor Proteins Proteins 0.000 description 2
- 102000009661 Repressor Proteins Human genes 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000006907 apoptotic process Effects 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 238000010923 batch production Methods 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 229960005091 chloramphenicol Drugs 0.000 description 2
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 2
- 238000011210 chromatographic step Methods 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 230000001276 controlling effect Effects 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 238000001784 detoxification Methods 0.000 description 2
- 230000002222 downregulating effect Effects 0.000 description 2
- 229940088598 enzyme Drugs 0.000 description 2
- 101150117187 glmS gene Proteins 0.000 description 2
- 230000009036 growth inhibition Effects 0.000 description 2
- 235000019514 herring Nutrition 0.000 description 2
- AIHDCSAXVMAMJH-GFBKWZILSA-N levan Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@@H]1[C@@H](O)[C@H](O)[C@](CO)(CO[C@@H]2[C@H]([C@H](O)[C@@](O)(CO)O2)O)O1 AIHDCSAXVMAMJH-GFBKWZILSA-N 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000035772 mutation Effects 0.000 description 2
- 231100000252 nontoxic Toxicity 0.000 description 2
- 230000003000 nontoxic effect Effects 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 230000036961 partial effect Effects 0.000 description 2
- 230000002085 persistent effect Effects 0.000 description 2
- 229920002401 polyacrylamide Polymers 0.000 description 2
- 230000003389 potentiating effect Effects 0.000 description 2
- 101150079601 recA gene Proteins 0.000 description 2
- 230000003362 replicative effect Effects 0.000 description 2
- 108091008146 restriction endonucleases Proteins 0.000 description 2
- 210000003705 ribosome Anatomy 0.000 description 2
- 230000001629 suppression Effects 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 239000003053 toxin Substances 0.000 description 2
- 231100000765 toxin Toxicity 0.000 description 2
- 108700012359 toxins Proteins 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- 101710192508 30S ribosomal protein S2 Proteins 0.000 description 1
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- 231100000699 Bacterial toxin Toxicity 0.000 description 1
- 206010004906 Biotin deficiency Diseases 0.000 description 1
- 241000908115 Bolivar Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241000722731 Carex Species 0.000 description 1
- 108010035563 Chloramphenicol O-acetyltransferase Proteins 0.000 description 1
- 108091026890 Coding region Proteins 0.000 description 1
- 108010054814 DNA Gyrase Proteins 0.000 description 1
- 102000004594 DNA Polymerase I Human genes 0.000 description 1
- 108010017826 DNA Polymerase I Proteins 0.000 description 1
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 1
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 1
- 102000007260 Deoxyribonuclease I Human genes 0.000 description 1
- 108010008532 Deoxyribonuclease I Proteins 0.000 description 1
- 108700034853 E coli TRPR Proteins 0.000 description 1
- 101150013191 E gene Proteins 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 101100285515 Escherichia coli (strain K12) hokD gene Proteins 0.000 description 1
- 241001646716 Escherichia coli K-12 Species 0.000 description 1
- 241000272186 Falco columbarius Species 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 102000029793 Isoleucine-tRNA ligase Human genes 0.000 description 1
- 101710176147 Isoleucine-tRNA ligase, cytoplasmic Proteins 0.000 description 1
- 241000588748 Klebsiella Species 0.000 description 1
- 108060001084 Luciferase Proteins 0.000 description 1
- 239000005089 Luciferase Substances 0.000 description 1
- 101150064138 MAP1 gene Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 101710181812 Methionine aminopeptidase Proteins 0.000 description 1
- 101100468245 Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) relF gene Proteins 0.000 description 1
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 108091092724 Noncoding DNA Proteins 0.000 description 1
- 241000701245 Paramecium bursaria Chlorella virus 1 Species 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 101710149031 Probable isoleucine-tRNA ligase, cytoplasmic Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- 101100075995 Schizosaccharomyces pombe (strain 972 / ATCC 24843) fma2 gene Proteins 0.000 description 1
- 206010070834 Sensitisation Diseases 0.000 description 1
- 101100443856 Streptococcus pyogenes serotype M18 (strain MGAS8232) polC gene Proteins 0.000 description 1
- 102000019197 Superoxide Dismutase Human genes 0.000 description 1
- 108010012715 Superoxide dismutase Proteins 0.000 description 1
- 108091029855 T44 RNA Proteins 0.000 description 1
- 102000004357 Transferases Human genes 0.000 description 1
- 108090000992 Transferases Proteins 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- LFTYTUAZOPRMMI-CFRASDGPSA-N UDP-N-acetyl-alpha-D-glucosamine Chemical compound O1[C@H](CO)[C@@H](O)[C@H](O)[C@@H](NC(=O)C)[C@H]1OP(O)(=O)OP(O)(=O)OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C(NC(=O)C=C2)=O)O1 LFTYTUAZOPRMMI-CFRASDGPSA-N 0.000 description 1
- LFTYTUAZOPRMMI-UHFFFAOYSA-N UNPD164450 Natural products O1C(CO)C(O)C(O)C(NC(=O)C)C1OP(O)(=O)OP(O)(=O)OCC1C(O)C(O)C(N2C(NC(=O)C=C2)=O)O1 LFTYTUAZOPRMMI-UHFFFAOYSA-N 0.000 description 1
- 241001672648 Vieira Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- DLRVVLDZNNYCBX-CAPXFGMSSA-N allolactose Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@@H]1OC[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](O)O1 DLRVVLDZNNYCBX-CAPXFGMSSA-N 0.000 description 1
- 108010013829 alpha subunit DNA polymerase III Proteins 0.000 description 1
- 238000005349 anion exchange Methods 0.000 description 1
- 238000005571 anion exchange chromatography Methods 0.000 description 1
- 239000005557 antagonist Substances 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 239000000688 bacterial toxin Substances 0.000 description 1
- 239000003782 beta lactam antibiotic agent Substances 0.000 description 1
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
- 239000004327 boric acid Substances 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 101150055766 cat gene Proteins 0.000 description 1
- 239000006143 cell culture medium Substances 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 230000002759 chromosomal effect Effects 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 230000009918 complex formation Effects 0.000 description 1
- 238000004590 computer program Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 101150027005 divIB gene Proteins 0.000 description 1
- 101150008507 dnaE gene Proteins 0.000 description 1
- 101150035285 dnaE1 gene Proteins 0.000 description 1
- 101150003155 dnaG gene Proteins 0.000 description 1
- 230000000464 effect on transcription Effects 0.000 description 1
- 230000000463 effect on translation Effects 0.000 description 1
- 101150012763 endA gene Proteins 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000009123 feedback regulation Effects 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 101150101609 ftsA gene Proteins 0.000 description 1
- 101150002100 ftsK gene Proteins 0.000 description 1
- 101150026421 ftsQ gene Proteins 0.000 description 1
- 101150111615 ftsZ gene Proteins 0.000 description 1
- 238000005227 gel permeation chromatography Methods 0.000 description 1
- 238000001476 gene delivery Methods 0.000 description 1
- 230000030279 gene silencing Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000006801 homologous recombination Effects 0.000 description 1
- 238000002744 homologous recombination Methods 0.000 description 1
- 101150023479 hsdS gene Proteins 0.000 description 1
- 210000003000 inclusion body Anatomy 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 238000013383 initial experiment Methods 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 230000002147 killing effect Effects 0.000 description 1
- 231100001231 less toxic Toxicity 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 101150112095 map gene Proteins 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000011987 methylation Effects 0.000 description 1
- 238000007069 methylation reaction Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 101150093139 ompT gene Proteins 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 210000001322 periplasm Anatomy 0.000 description 1
- 230000002028 premature Effects 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 210000001236 prokaryotic cell Anatomy 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000012743 protein tagging Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 1
- 238000004366 reverse phase liquid chromatography Methods 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 108020004418 ribosomal RNA Proteins 0.000 description 1
- 101150078369 rpsB gene Proteins 0.000 description 1
- 239000012146 running buffer Substances 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000010187 selection method Methods 0.000 description 1
- 230000008313 sensitization Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- 230000010474 transient expression Effects 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 230000003827 upregulation Effects 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000002132 β-lactam antibiotic Substances 0.000 description 1
- 229940124586 β-lactam antibiotics Drugs 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP04022201.0 | 2004-09-17 | ||
| EP04022201 | 2004-09-17 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR20077007467A Division KR101023066B1 (ko) | 2004-09-17 | 2005-09-08 | 항생제-없는 ＣｏｌＥ１ 플라스미드 증식을 위한 숙주-벡터시스템 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR20100101689A true KR20100101689A (ko) | 2010-09-17 |
Family
ID=34926585
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020107017286A Ceased KR20100101689A (ko) | 2004-09-17 | 2005-09-08 | 항생제-없는 ＣｏｌＥ１ 플라스미드 증식을 위한 숙주-벡터 시스템 |
| KR20077007467A Expired - Lifetime KR101023066B1 (ko) | 2004-09-17 | 2005-09-08 | 항생제-없는 ＣｏｌＥ１ 플라스미드 증식을 위한 숙주-벡터시스템 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR20077007467A Expired - Lifetime KR101023066B1 (ko) | 2004-09-17 | 2005-09-08 | 항생제-없는 ＣｏｌＥ１ 플라스미드 증식을 위한 숙주-벡터시스템 |
Country Status (13)
| Country | Link |
|---|---|
| US (2) | US20060063232A1 (https=) |
| EP (1) | EP1794299B1 (https=) |
| JP (1) | JP5301832B2 (https=) |
| KR (2) | KR20100101689A (https=) |
| CN (2) | CN101052722A (https=) |
| AR (2) | AR050649A1 (https=) |
| AU (1) | AU2005284136B2 (https=) |
| BR (1) | BRPI0515436A (https=) |
| CA (1) | CA2580009C (https=) |
| IL (1) | IL181919A (https=) |
| NZ (1) | NZ554236A (https=) |
| TW (1) | TWI311152B (https=) |
| WO (1) | WO2006029985A2 (https=) |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0327056D0 (en) * | 2003-11-20 | 2003-12-24 | Cobra Biolog Ltd | Plasmid maintenance |
| WO2008142028A1 (en) | 2007-05-17 | 2008-11-27 | Boehringer Ingelheim Rcv Gmbh & Co Kg | Method for producing a recombinant protein on a manufacturing scale |
| EP2333091B1 (en) | 2007-05-29 | 2017-06-21 | Nature Technology Corporation | Vectors and methods for genetic immunization |
| WO2009011651A1 (en) * | 2007-07-16 | 2009-01-22 | Rvc Enterprise | Mutually suppressive gene/inhibitor combinations for non-antibiotic selection of recombinant strains |
| EP2500427B1 (en) | 2007-11-22 | 2014-07-30 | Japan Science and Technology Agency | Translation regulation system in cell or artifical cell model by using low-molecular-weight RNA |
| JP5538691B2 (ja) * | 2007-11-22 | 2014-07-02 | 独立行政法人科学技術振興機構 | Ｒｎａ−蛋白質相互作用モチーフを利用した蛋白質応答翻訳制御システム |
| NZ596453A (en) * | 2009-05-22 | 2013-09-27 | Merial Ltd | Antibiotic-free plasmid |
| US20110269184A1 (en) | 2009-07-16 | 2011-11-03 | Boehringer Ingelheim Rcv Gmbh & Co. Kg | Method for controlling plasmid copy number in e.coli |
| US20130266983A1 (en) * | 2010-12-14 | 2013-10-10 | The Regents Of The University Of California | Creation of super-stable ColE1 plasmids by duplication of SL1-4 sequence and point mutations. |
| GB201021795D0 (en) * | 2010-12-21 | 2011-02-02 | Msd Biolog Uk Ltd | Expression Process |
| AU2013309488A1 (en) | 2012-08-29 | 2015-03-05 | Nature Technology Corporation | DNA plasmids with improved expression |
| WO2014077863A1 (en) | 2012-11-19 | 2014-05-22 | Nature Technology Corporation | Replicative minicircle vectors with improved expression |
| ES2940620T3 (es) * | 2016-11-02 | 2023-05-09 | Archerdx Llc | Métodos de preparación de muestras de ácido nucleico para la secuenciación del repertorio inmunitario |
| CN107142272A (zh) * | 2017-06-05 | 2017-09-08 | 南京金斯瑞生物科技有限公司 | 一种控制大肠杆菌中质粒复制的方法 |
| DK3456821T4 (da) | 2017-09-19 | 2024-04-15 | Deutsches Krebsforsch | Ikke-integrerende dna-vektorer til genetisk modifikation af celler |
| WO2019057774A1 (en) | 2017-09-19 | 2019-03-28 | Deutsches Krebsforschungszentrum | NON-INTEGRATING DNA VECTORS FOR THE GENETIC MODIFICATION OF CELLS |
| DK3768846T5 (da) | 2018-03-21 | 2024-08-19 | Aldevron L L C | Virale og ikke-virale nanoplasmidvektorer med forbedret produktion |
| JP7661015B2 (ja) | 2020-06-12 | 2025-04-14 | ナガセヴィータ株式会社 | 目的タンパク質の製造方法 |
| JP2025507940A (ja) * | 2022-03-04 | 2025-03-21 | ゲン－ハー ジェネティック エンジニアリング ハイデルベルク ゲーエムベーハー | 抗生物質を含まないプラスミドベースの発酵のための微生物株及びその生成方法 |
| CN117187281A (zh) * | 2023-09-11 | 2023-12-08 | 云舟生物科技(广州)股份有限公司 | 一种无抗性质粒生产系统及其应用 |
| CN118028194B (zh) * | 2023-12-26 | 2024-09-06 | 浙江大学 | 基因组精简的恶臭假单胞菌菌株 |
| WO2025160245A1 (en) | 2024-01-25 | 2025-07-31 | Aldevron, L.L.C. | Viral and non-viral nanoplasmid vectors with improved production |
| WO2025231315A2 (en) | 2024-05-03 | 2025-11-06 | Aldevron, L.L.C. | Bacterial host strains |
| WO2026076187A1 (en) | 2024-10-03 | 2026-04-09 | Aldevron, L.L.C. | Compositions and methods for microbial production of antibiotic-free closed-ended linear dna |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9215541D0 (en) * | 1992-07-22 | 1992-09-02 | Celltech Ltd | Protein expression system |
| US5922583A (en) * | 1995-10-17 | 1999-07-13 | Biostar Inc. | Methods for production of recombinant plasmids |
| EP1195436A1 (en) | 2000-10-04 | 2002-04-10 | BOEHRINGER INGELHEIM INTERNATIONAL GmbH | Expression vectors with modified ColE1 origin of replication for control of plasmid copy number |
| JP2004533827A (ja) * | 2001-05-17 | 2004-11-11 | アールフス・ユニバーシティー | 遺伝子治療用の改良されたベクター |
| SE0102204D0 (sv) * | 2001-06-21 | 2001-06-21 | Leif Isaksson | New method |
| CN1490056A (zh) * | 2002-10-18 | 2004-04-21 | ��¡���ɵ°��̲��о����� | 针对hiv-1的免疫方法和组合物 |
| GB0327056D0 (en) * | 2003-11-20 | 2003-12-24 | Cobra Biolog Ltd | Plasmid maintenance |
-
2005
- 2005-09-06 TW TW94130459A patent/TWI311152B/zh not_active IP Right Cessation
- 2005-09-08 CA CA 2580009 patent/CA2580009C/en not_active Expired - Lifetime
- 2005-09-08 EP EP05789661.5A patent/EP1794299B1/en not_active Expired - Lifetime
- 2005-09-08 BR BRPI0515436-7A patent/BRPI0515436A/pt not_active IP Right Cessation
- 2005-09-08 NZ NZ554236A patent/NZ554236A/en not_active IP Right Cessation
- 2005-09-08 CN CNA2005800377789A patent/CN101052722A/zh active Pending
- 2005-09-08 KR KR1020107017286A patent/KR20100101689A/ko not_active Ceased
- 2005-09-08 WO PCT/EP2005/054450 patent/WO2006029985A2/en not_active Ceased
- 2005-09-08 JP JP2007531743A patent/JP5301832B2/ja not_active Expired - Lifetime
- 2005-09-08 AU AU2005284136A patent/AU2005284136B2/en not_active Expired
- 2005-09-08 CN CN201310409070.8A patent/CN103555644B/zh not_active Expired - Lifetime
- 2005-09-08 KR KR20077007467A patent/KR101023066B1/ko not_active Expired - Lifetime
- 2005-09-14 US US11/226,795 patent/US20060063232A1/en not_active Abandoned
- 2005-09-19 AR ARP050103886 patent/AR050649A1/es not_active Application Discontinuation
-
2007
- 2007-03-14 IL IL181919A patent/IL181919A/en not_active IP Right Cessation
-
2008
- 2008-10-28 US US12/259,662 patent/US9297014B2/en active Active
-
2011
- 2011-01-21 AR ARP110100203 patent/AR079958A2/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| KR20070059130A (ko) | 2007-06-11 |
| CN103555644B (zh) | 2018-06-01 |
| CN101052722A (zh) | 2007-10-10 |
| JP5301832B2 (ja) | 2013-09-25 |
| CA2580009C (en) | 2013-12-17 |
| EP1794299B1 (en) | 2015-09-02 |
| NZ554236A (en) | 2010-05-28 |
| TWI311152B (en) | 2009-06-21 |
| JP2008512998A (ja) | 2008-05-01 |
| AU2005284136A1 (en) | 2006-03-23 |
| US9297014B2 (en) | 2016-03-29 |
| AR079958A2 (es) | 2012-02-29 |
| CN103555644A (zh) | 2014-02-05 |
| CA2580009A1 (en) | 2006-03-23 |
| WO2006029985A3 (en) | 2006-07-27 |
| US20060063232A1 (en) | 2006-03-23 |
| IL181919A (en) | 2012-04-30 |
| KR101023066B1 (ko) | 2011-03-24 |
| TW200621985A (en) | 2006-07-01 |
| AU2005284136B2 (en) | 2011-07-14 |
| IL181919A0 (en) | 2007-07-04 |
| WO2006029985A2 (en) | 2006-03-23 |
| BRPI0515436A (pt) | 2008-07-22 |
| AR050649A1 (es) | 2006-11-08 |
| US20090263861A1 (en) | 2009-10-22 |
| EP1794299A2 (en) | 2007-06-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR101023066B1 (ko) | 항생제-없는 ＣｏｌＥ１ 플라스미드 증식을 위한 숙주-벡터시스템 | |
| US9012226B2 (en) | Bacterial strains with improved plasmid stability | |
| EP2284273A1 (en) | Vector comprising mannose promoter and mannose promoter | |
| KR102688555B1 (ko) | CRISPR/Cpf1 시스템을 기반으로 한 유전체 단일 염기 편집 방법 및 이의 용도 | |
| CN102757975A (zh) | 一种增加龟裂链霉菌的土霉素产量的方法 | |
| EP2722390B1 (en) | Regulation of inducible promoters | |
| EP1924693B1 (en) | Hybrid portable origin of replication plasmids | |
| PL222067B1 (pl) | Kaseta ekspresyjna, zastosowanie kasety ekspresyjnej, wektor ekspresyjny, komórka prokariotycznego gospodarza zawierająca wektor ekspresyjny, szczep bakteryjny oraz sposób wytwarzania polipeptydu | |
| CN118995777A (zh) | 金属离子转运调控基因sco2508在提升天蓝色链霉菌中放线紫红素产量中的应用 | |
| CN120683099A (zh) | 一种用于链霉菌的高效基因编辑系统及其构建方法和应用 | |
| US20110269184A1 (en) | Method for controlling plasmid copy number in e.coli | |
| CN101305096B (zh) | 新选择体系 | |
| CN103497965A (zh) | 一种温控零背景t载体前体及其构建方法和应用 | |
| CA2549263A1 (en) | Systems for tightly regulated gene expression | |
| CN114450408A (zh) | 大载体和用于高产量生产的方法 | |
| MX2007003110A (en) | Host-vector system for antibiotic-free cole1 plasmid propagation | |
| CN110218701B (zh) | 一种筛选miRNA调控剂的细胞模型及应用 | |
| KR20250042179A (ko) | CRISPR-Cas9-기반의 플라스미드 DNA 주형의 선형화 | |
| Geist et al. | TraM protein of plasmid R1: in vitro selection of the target region reveals two consensus 7 bp binding motifs spaced by a 4 bp linker of defined sequence | |
| CN117178056A (zh) | 用于生产无缝dna载体的方法 | |
| CN104046648A (zh) | 大肠杆菌-链霉菌穿梭型bac载体及其构建方法 | |
| Zolotukhina et al. | Mutational Analysis of a Leader Region of the Escherichia coli ribB Gene |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A107 | Divisional application of patent | ||
| A201 | Request for examination | ||
| PA0104 | Divisional application for international application |
Comment text: Divisional Application for International Patent Patent event code: PA01041R01D Patent event date: 20100802 |
|
| PA0201 | Request for examination | ||
| PG1501 | Laying open of application | ||
| E902 | Notification of reason for refusal | ||
| PE0902 | Notice of grounds for rejection |
Comment text: Notification of reason for refusal Patent event date: 20101021 Patent event code: PE09021S01D |
|
| E601 | Decision to refuse application | ||
| PE0601 | Decision on rejection of patent |
Patent event date: 20110628 Comment text: Decision to Refuse Application Patent event code: PE06012S01D Patent event date: 20101021 Comment text: Notification of reason for refusal Patent event code: PE06011S01I |