CN101052722A - 用于无抗生素ColE1质粒增殖的宿主－载体系统 - Google Patents

用于无抗生素ColE1质粒增殖的宿主－载体系统 Download PDF

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Publication number
CN101052722A
CN101052722A CNA2005800377789A CN200580037778A CN101052722A CN 101052722 A CN101052722 A CN 101052722A CN A2005800377789 A CNA2005800377789 A CN A2005800377789A CN 200580037778 A CN200580037778 A CN 200580037778A CN 101052722 A CN101052722 A CN 101052722A
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China
Prior art keywords
plasmid
dna
rna
cell
sequence
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Pending
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CNA2005800377789A
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English (en)
Chinese (zh)
Inventor
莱因加德·格拉贝尔
艾琳·普法芬泽勒
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Boehringer Ingelheim RCV GmbH and Co KG
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Boehringer Ingelheim Austria GmbH
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Application filed by Boehringer Ingelheim Austria GmbH filed Critical Boehringer Ingelheim Austria GmbH
Priority to CN201310409070.8A priority Critical patent/CN103555644B/zh
Publication of CN101052722A publication Critical patent/CN101052722A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CNA2005800377789A 2004-09-17 2005-09-08 用于无抗生素ColE1质粒增殖的宿主－载体系统 Pending CN101052722A (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201310409070.8A CN103555644B (zh) 2004-09-17 2005-09-08 用于无抗生素ColE1 质粒增殖的宿主-载体系统

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP04022201.0 2004-09-17
EP04022201 2004-09-17

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN201310409070.8A Division CN103555644B (zh) 2004-09-17 2005-09-08 用于无抗生素ColE1 质粒增殖的宿主-载体系统

Publications (1)

Publication Number Publication Date
CN101052722A true CN101052722A (zh) 2007-10-10

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ID=34926585

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CNA2005800377789A Pending CN101052722A (zh) 2004-09-17 2005-09-08 用于无抗生素ColE1质粒增殖的宿主－载体系统
CN201310409070.8A Expired - Lifetime CN103555644B (zh) 2004-09-17 2005-09-08 用于无抗生素ColE1 质粒增殖的宿主-载体系统

Family Applications After (1)

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CN201310409070.8A Expired - Lifetime CN103555644B (zh) 2004-09-17 2005-09-08 用于无抗生素ColE1 质粒增殖的宿主-载体系统

Country Status (13)

Country Link
US (2) US20060063232A1 (https=)
EP (1) EP1794299B1 (https=)
JP (1) JP5301832B2 (https=)
KR (2) KR20100101689A (https=)
CN (2) CN101052722A (https=)
AR (2) AR050649A1 (https=)
AU (1) AU2005284136B2 (https=)
BR (1) BRPI0515436A (https=)
CA (1) CA2580009C (https=)
IL (1) IL181919A (https=)
NZ (1) NZ554236A (https=)
TW (1) TWI311152B (https=)
WO (1) WO2006029985A2 (https=)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0327056D0 (en) * 2003-11-20 2003-12-24 Cobra Biolog Ltd Plasmid maintenance
WO2008142028A1 (en) 2007-05-17 2008-11-27 Boehringer Ingelheim Rcv Gmbh & Co Kg Method for producing a recombinant protein on a manufacturing scale
EP2333091B1 (en) 2007-05-29 2017-06-21 Nature Technology Corporation Vectors and methods for genetic immunization
WO2009011651A1 (en) * 2007-07-16 2009-01-22 Rvc Enterprise Mutually suppressive gene/inhibitor combinations for non-antibiotic selection of recombinant strains
EP2500427B1 (en) 2007-11-22 2014-07-30 Japan Science and Technology Agency Translation regulation system in cell or artifical cell model by using low-molecular-weight RNA
JP5538691B2 (ja) * 2007-11-22 2014-07-02 独立行政法人科学技術振興機構 Ｒｎａ−蛋白質相互作用モチーフを利用した蛋白質応答翻訳制御システム
NZ596453A (en) * 2009-05-22 2013-09-27 Merial Ltd Antibiotic-free plasmid
US20110269184A1 (en) 2009-07-16 2011-11-03 Boehringer Ingelheim Rcv Gmbh & Co. Kg Method for controlling plasmid copy number in e.coli
US20130266983A1 (en) * 2010-12-14 2013-10-10 The Regents Of The University Of California Creation of super-stable ColE1 plasmids by duplication of SL1-4 sequence and point mutations.
GB201021795D0 (en) * 2010-12-21 2011-02-02 Msd Biolog Uk Ltd Expression Process
AU2013309488A1 (en) 2012-08-29 2015-03-05 Nature Technology Corporation DNA plasmids with improved expression
WO2014077863A1 (en) 2012-11-19 2014-05-22 Nature Technology Corporation Replicative minicircle vectors with improved expression
ES2940620T3 (es) * 2016-11-02 2023-05-09 Archerdx Llc Métodos de preparación de muestras de ácido nucleico para la secuenciación del repertorio inmunitario
CN107142272A (zh) * 2017-06-05 2017-09-08 南京金斯瑞生物科技有限公司 一种控制大肠杆菌中质粒复制的方法
DK3456821T4 (da) 2017-09-19 2024-04-15 Deutsches Krebsforsch Ikke-integrerende dna-vektorer til genetisk modifikation af celler
WO2019057774A1 (en) 2017-09-19 2019-03-28 Deutsches Krebsforschungszentrum NON-INTEGRATING DNA VECTORS FOR THE GENETIC MODIFICATION OF CELLS
DK3768846T5 (da) 2018-03-21 2024-08-19 Aldevron L L C Virale og ikke-virale nanoplasmidvektorer med forbedret produktion
JP7661015B2 (ja) 2020-06-12 2025-04-14 ナガセヴィータ株式会社 目的タンパク質の製造方法
JP2025507940A (ja) * 2022-03-04 2025-03-21 ゲン－ハー ジェネティック エンジニアリング ハイデルベルク ゲーエムベーハー 抗生物質を含まないプラスミドベースの発酵のための微生物株及びその生成方法
CN117187281A (zh) * 2023-09-11 2023-12-08 云舟生物科技(广州)股份有限公司 一种无抗性质粒生产系统及其应用
CN118028194B (zh) * 2023-12-26 2024-09-06 浙江大学 基因组精简的恶臭假单胞菌菌株
WO2025160245A1 (en) 2024-01-25 2025-07-31 Aldevron, L.L.C. Viral and non-viral nanoplasmid vectors with improved production
WO2025231315A2 (en) 2024-05-03 2025-11-06 Aldevron, L.L.C. Bacterial host strains
WO2026076187A1 (en) 2024-10-03 2026-04-09 Aldevron, L.L.C. Compositions and methods for microbial production of antibiotic-free closed-ended linear dna

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9215541D0 (en) * 1992-07-22 1992-09-02 Celltech Ltd Protein expression system
US5922583A (en) * 1995-10-17 1999-07-13 Biostar Inc. Methods for production of recombinant plasmids
EP1195436A1 (en) 2000-10-04 2002-04-10 BOEHRINGER INGELHEIM INTERNATIONAL GmbH Expression vectors with modified ColE1 origin of replication for control of plasmid copy number
JP2004533827A (ja) * 2001-05-17 2004-11-11 アールフス・ユニバーシティー 遺伝子治療用の改良されたベクター
SE0102204D0 (sv) * 2001-06-21 2001-06-21 Leif Isaksson New method
CN1490056A (zh) * 2002-10-18 2004-04-21 ��¡���ɵ°��̲��о����� 针对hiv-1的免疫方法和组合物
GB0327056D0 (en) * 2003-11-20 2003-12-24 Cobra Biolog Ltd Plasmid maintenance

Also Published As

Publication number Publication date
KR20070059130A (ko) 2007-06-11
CN103555644B (zh) 2018-06-01
JP5301832B2 (ja) 2013-09-25
KR20100101689A (ko) 2010-09-17
CA2580009C (en) 2013-12-17
EP1794299B1 (en) 2015-09-02
NZ554236A (en) 2010-05-28
TWI311152B (en) 2009-06-21
JP2008512998A (ja) 2008-05-01
AU2005284136A1 (en) 2006-03-23
US9297014B2 (en) 2016-03-29
AR079958A2 (es) 2012-02-29
CN103555644A (zh) 2014-02-05
CA2580009A1 (en) 2006-03-23
WO2006029985A3 (en) 2006-07-27
US20060063232A1 (en) 2006-03-23
IL181919A (en) 2012-04-30
KR101023066B1 (ko) 2011-03-24
TW200621985A (en) 2006-07-01
AU2005284136B2 (en) 2011-07-14
IL181919A0 (en) 2007-07-04
WO2006029985A2 (en) 2006-03-23
BRPI0515436A (pt) 2008-07-22
AR050649A1 (es) 2006-11-08
US20090263861A1 (en) 2009-10-22
EP1794299A2 (en) 2007-06-13

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Application publication date: 20071010