AU2005284136B2 - Host-vector system for antibiotic-free ColE1 plasmid propagation - Google Patents

Host-vector system for antibiotic-free ColE1 plasmid propagation Download PDF

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Publication number
AU2005284136B2
AU2005284136B2 AU2005284136A AU2005284136A AU2005284136B2 AU 2005284136 B2 AU2005284136 B2 AU 2005284136B2 AU 2005284136 A AU2005284136 A AU 2005284136A AU 2005284136 A AU2005284136 A AU 2005284136A AU 2005284136 B2 AU2005284136 B2 AU 2005284136B2
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AU
Australia
Prior art keywords
plasmid
rna
protein
gene
host
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Expired
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AU2005284136A
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English (en)
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AU2005284136A1 (en
Inventor
Reingard Grabherr
Irene Pfaffenzeller
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Boehringer Ingelheim RCV GmbH and Co KG
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Boehringer Ingelheim RCV GmbH and Co KG
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Application filed by Boehringer Ingelheim RCV GmbH and Co KG filed Critical Boehringer Ingelheim RCV GmbH and Co KG
Publication of AU2005284136A1 publication Critical patent/AU2005284136A1/en
Assigned to Boehringer Ingelheim Pharma RCV GmbH & Co KG reassignment Boehringer Ingelheim Pharma RCV GmbH & Co KG Alteration of Name(s) of Applicant(s) under S113 Assignors: BOEHRINGER INGELHEIM AUSTRIA GMBH
Application granted granted Critical
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2005284136A 2004-09-17 2005-09-08 Host-vector system for antibiotic-free ColE1 plasmid propagation Expired AU2005284136B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP04022201.0 2004-09-17
EP04022201 2004-09-17
PCT/EP2005/054450 WO2006029985A2 (en) 2004-09-17 2005-09-08 Host-vector system for antibiotic-free cole1 plasmid propagation

Publications (2)

Publication Number Publication Date
AU2005284136A1 AU2005284136A1 (en) 2006-03-23
AU2005284136B2 true AU2005284136B2 (en) 2011-07-14

Family

ID=34926585

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2005284136A Expired AU2005284136B2 (en) 2004-09-17 2005-09-08 Host-vector system for antibiotic-free ColE1 plasmid propagation

Country Status (13)

Country Link
US (2) US20060063232A1 (https=)
EP (1) EP1794299B1 (https=)
JP (1) JP5301832B2 (https=)
KR (2) KR101023066B1 (https=)
CN (2) CN103555644B (https=)
AR (2) AR050649A1 (https=)
AU (1) AU2005284136B2 (https=)
BR (1) BRPI0515436A (https=)
CA (1) CA2580009C (https=)
IL (1) IL181919A (https=)
NZ (1) NZ554236A (https=)
TW (1) TWI311152B (https=)
WO (1) WO2006029985A2 (https=)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0327056D0 (en) * 2003-11-20 2003-12-24 Cobra Biolog Ltd Plasmid maintenance
JP5837747B2 (ja) * 2007-05-17 2015-12-24 ベーリンガー インゲルハイム エルツェーファウ ゲゼルシャフト ミット ベシュレンクテル ハフツング ウント コンパニー コマンディトゲゼルシャフト 組換えタンパク質を工業規模で製造する方法
EP2152889B1 (en) 2007-05-29 2017-03-15 Nature Technology Corporation Vectors and methods for genetic immunization
WO2009011651A1 (en) * 2007-07-16 2009-01-22 Rvc Enterprise Mutually suppressive gene/inhibitor combinations for non-antibiotic selection of recombinant strains
JP5538691B2 (ja) * 2007-11-22 2014-07-02 独立行政法人科学技術振興機構 Ｒｎａ−蛋白質相互作用モチーフを利用した蛋白質応答翻訳制御システム
EP2221371B1 (en) * 2007-11-22 2013-11-13 Japan Science and Technology Agency Translation regulation system in cell or artificial cell model by using low-molecular-weight rna
RU2548809C2 (ru) * 2009-05-22 2015-04-20 Мериал Лимитед Плазмида без устойчивости к антибиотику
US20110269184A1 (en) 2009-07-16 2011-11-03 Boehringer Ingelheim Rcv Gmbh & Co. Kg Method for controlling plasmid copy number in e.coli
WO2012082946A2 (en) * 2010-12-14 2012-06-21 The Regents Of The University Of California Creation of super-stable coie1 plasmids by duplication of sl 1-4 sequence and point mutations.
GB201021795D0 (en) * 2010-12-21 2011-02-02 Msd Biolog Uk Ltd Expression Process
US9550998B2 (en) 2012-08-29 2017-01-24 Nature Technology Corporation DNA plasmids with improved expression
US20150322439A1 (en) 2012-11-19 2015-11-12 Nature Technology Corporation Replicative minicircle vectors with improved expression
EP4198140B1 (en) * 2016-11-02 2024-09-04 ArcherDX, LLC Methods of nucleic acid sample preparation for immune repertoire sequencing
CN107142272A (zh) * 2017-06-05 2017-09-08 南京金斯瑞生物科技有限公司 一种控制大肠杆菌中质粒复制的方法
EP3456821B2 (en) 2017-09-19 2024-01-24 Deutsches Krebsforschungszentrum Non-integrating dna vectors for the genetic modification of cells
WO2019057774A1 (en) 2017-09-19 2019-03-28 Deutsches Krebsforschungszentrum NON-INTEGRATING DNA VECTORS FOR THE GENETIC MODIFICATION OF CELLS
WO2019183248A1 (en) 2018-03-21 2019-09-26 Nature Technology Corporation Viral and non-viral nanoplasmid vectors with improved production
JP7661015B2 (ja) * 2020-06-12 2025-04-14 ナガセヴィータ株式会社 目的タンパク質の製造方法
WO2023166132A1 (en) * 2022-03-04 2023-09-07 Gen-H Genetic Engineering Heidelberg Gmbh Microorganism strain for antibiotic-free plasmid-based fermentation and method for generation thereof
CN117187281A (zh) * 2023-09-11 2023-12-08 云舟生物科技(广州)股份有限公司 一种无抗性质粒生产系统及其应用
CN118028194B (zh) * 2023-12-26 2024-09-06 浙江大学 基因组精简的恶臭假单胞菌菌株
WO2025160245A1 (en) 2024-01-25 2025-07-31 Aldevron, L.L.C. Viral and non-viral nanoplasmid vectors with improved production
WO2025231315A2 (en) 2024-05-03 2025-11-06 Aldevron, L.L.C. Bacterial host strains
WO2026076187A1 (en) 2024-10-03 2026-04-09 Aldevron, L.L.C. Compositions and methods for microbial production of antibiotic-free closed-ended linear dna

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005052167A2 (en) * 2003-11-20 2005-06-09 Cobra Biologics Limited Plasmid maintenance

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9215541D0 (en) * 1992-07-22 1992-09-02 Celltech Ltd Protein expression system
US5922583A (en) * 1995-10-17 1999-07-13 Biostar Inc. Methods for production of recombinant plasmids
EP1195436A1 (en) 2000-10-04 2002-04-10 BOEHRINGER INGELHEIM INTERNATIONAL GmbH Expression vectors with modified ColE1 origin of replication for control of plasmid copy number
WO2002092825A2 (en) * 2001-05-17 2002-11-21 Aarhus Universitet Retroviral vectors for gene therapy
SE0102204D0 (sv) * 2001-06-21 2001-06-21 Leif Isaksson New method
CN1490056A (zh) * 2002-10-18 2004-04-21 ��¡���ɵ°��̲��о����� 针对hiv-1的免疫方法和组合物

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005052167A2 (en) * 2003-11-20 2005-06-09 Cobra Biologics Limited Plasmid maintenance

Also Published As

Publication number Publication date
KR101023066B1 (ko) 2011-03-24
JP2008512998A (ja) 2008-05-01
CN103555644B (zh) 2018-06-01
AU2005284136A1 (en) 2006-03-23
KR20100101689A (ko) 2010-09-17
EP1794299A2 (en) 2007-06-13
WO2006029985A2 (en) 2006-03-23
TW200621985A (en) 2006-07-01
IL181919A0 (en) 2007-07-04
CN101052722A (zh) 2007-10-10
US20090263861A1 (en) 2009-10-22
NZ554236A (en) 2010-05-28
US9297014B2 (en) 2016-03-29
US20060063232A1 (en) 2006-03-23
AR079958A2 (es) 2012-02-29
BRPI0515436A (pt) 2008-07-22
CA2580009A1 (en) 2006-03-23
JP5301832B2 (ja) 2013-09-25
TWI311152B (en) 2009-06-21
WO2006029985A3 (en) 2006-07-27
AR050649A1 (es) 2006-11-08
EP1794299B1 (en) 2015-09-02
KR20070059130A (ko) 2007-06-11
IL181919A (en) 2012-04-30
CN103555644A (zh) 2014-02-05
CA2580009C (en) 2013-12-17

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Free format text: IN VOL 23, NO 1, PAGE(S) 6009 UNDER THE HEADING CHANGE OF NAMES(S) OF APPLICANT(S), SECTION 104 - 2005 UNDER THE NAME BOEHRINGER INGELHEIM PHARMA RCV GMBH & CO KG, APPLICATION NO. 2005284136, UNDER INID (71) CORRECT THE NAME TO READ BOEHRINGER INGELHEIM RCV GMBH & CO KG

FGA Letters patent sealed or granted (standard patent)
MK14 Patent ceased section 143(a) (annual fees not paid) or expired