KR20090021844A - Additive for fishes feed using photosynthetic bacteria and feed comprising it - Google Patents

Abstract

A feed additive for a fish using photosynthetic bacteria is provided to promote growth of a fish, improving an immunity system, and enhance quality of water by using a rodopsudomonas rutila. A feed additive for a fish using photosynthetic bacteria includes water having mineral and organized germanium, a rodopsudomonas rutila as photosynthetic bacteria, at least one protein source, at least one carbohydrate source, and at least one oil source. The protein source is selected from one group consisting of fish powder, casein, meat bone powder, and shrimp powder. The carbohydrate source is selected from one group consisting of sweet potato starch, potato starch, and wheat starch. The oil source is selected from one group consisting of rape oil, soybean oil, corn oil, pollack liver oil and squid oil.

Description

Additive for fishes feed using photosynthetic bacteria and feed comprising it}

The present invention relates to a feed additive and feed for fish using photosynthetic bacteria, and more particularly, by using Rhodoshudomonas rutira, which is most suitable for fish culture among photosynthetic bacteria, the effects of promoting fish growth and immunity enhancement and water quality improvement. It relates to feed additives and feed having an effect.

Aquaculture refers to the harvesting of aquatic organisms, which are necessary for human life, in the same way as onshore agriculture, which is called aquatic farming.

The origin of aquaculture is quite old, and there is a record that the king of Egypt made edible foods around 1800 B.C.E. It is not clear when Korean farming started, but it was said that Taein-do fishermen did farming when they were artificial.

Since then, steady research and development has been carried out, and now modernized forms are carried out nationwide.

In Korea, the three sides are surrounded by the sea, and the sea characteristics such as the water temperature and the topography of the south-western coast are different, so it is possible to grow various fish species according to each sea area. It is advantageous.

However, the domestic aquaculture industry is currently experiencing environmental factors due to the rapid quantitative expansion and the introduction of new fish species as well as environmental factors caused by the deterioration of water pollution due to indiscriminate granulation of farms and excessive use of various disinfectant disinfectants such as formalin and hydrogen peroxide. Biological factors such as inflow and emergence, the emergence of resistant bacteria due to the abuse of various antibiotics, not only high water temperature, but also sudden death during the year causes serious damage to many farms.

Research has been conducted on feed additives that enhance fish immunity and promote growth.

In recent years, the aquaculture industry has been focused on the development of a new form of fish feed or feed additives using photosynthetic bacteria to solve the above problems.

Photosynthetic bacteria are a group of important microorganisms in the natural plant circulation. They purify dirt, such as human and animal feces, as heterotrophic bacteria, and remove harmful substances in crops and fish such as hydrogen sulfide and nitrogen compounds. While ingestion is removed by food, it performs photosynthesis using carbon dioxide gas and solar energy, and promotes the growth of beneficial microorganisms such as lactic acid bacteria and actinomycetes in the soil, and increases the occurrence and proliferation of green microorganisms such as chlorella in the water. Used as food for zooplankton.

Korean Republic Patent No. 0454228 discloses a feed additive for aquaculture fish.

The feed additive disclosed above contains 0.01 to 3% by weight of polymer chitosan or salt thereof, 5 to 15% by weight of photosynthetic bacteria, 10 to 20% by weight of activated carbon, 10 to 20% by weight of illite, and 40 to 60% by weight of cereal husks. In addition, the disclosed feed additive is complicated in its composition, in particular, chitosan contained in the feed additive has an antimicrobial effect and binds to the cell membrane of the microorganism, thereby inhibiting the growth of the microorganism is a problem.

In addition, there are about 37 species of photosynthetic bacteria that have a red color in nature, and among them, there are 16 kinds of photosynthetic bacteria that are most used for crops, livestock, and fish farming. These red photosynthetic bacteria differ greatly in their characteristics, including nutrients, antimicrobial production, odor removal, and organic degradation. In addition, even in the same species, the nutritional substances to be released in the culture medium vary depending on the environment and the type of medium.

Photosynthetic bacteria used in the above-described feed additives are not the most suitable bacteria for fish farming because the type and culture environment of the medium are not specified, and the effects are questioned.

Therefore, the present inventors have developed feed additives and feeds using photosynthetic bacteria most suitable for fish farming in order to improve productivity and enhance competitiveness in fish farming based on many years of fish farming experience and technology.

The present invention was created to improve the above problems, by cultivating the photosynthetic bacteria most suitable for fish culture in the optimum medium to promote the growth of the fish and enhance the immunity only by the characteristics of photosynthetic bacteria to increase the weight gain and productivity increase The purpose is to provide feed additives and feed for fish.

Another object of the present invention is to provide a feed additive and feed capable of mass-dense farming by improving the water quality by effectively improving water quality by using photosynthetic bacteria having excellent water purification ability and increasing the density of aquaculture.

Fish feed additive using the photosynthetic bacteria of the present invention for achieving the above object is characterized in that it comprises photosynthetic bacteria Rhodoshudomonas rutira and water.

The water is characterized in that it further comprises a mineral.

The water is characterized in that it further comprises a blood powder containing organic germanium.

Fish feed using the photosynthetic bacteria of the present invention for achieving the above object is characterized in that it comprises any one of the above feed additives.

In addition to the feed additive, any one or more protein sources selected from fish meal, casein, meat bone meal, shrimp powder, any one or more carbohydrate sources selected from sweet potato starch, potato starch, wheat starch, rapeseed oil, soybean oil, corn oil, pollock It is characterized in that it further comprises any one or more lipid sources selected from liver oil, squid liver oil.

Fish feed additives and feed for fish using photosynthetic bacteria according to the present invention are high in the content of essential nutrients such as proteins, amino acids, vitamins and excellent antibacterial ability, the growth of fish by using the photosynthetic bacteria, the most suitable photosynthetic bacteria for fish farming It can increase the productivity of farmed fish by promoting and boosting immunity.

In addition, the feed additives and feed of the present invention is excellent in water quality and antimicrobial activity to enable large-scale dense farming of cultured fish.

In addition, by adding more minerals and organic germanium to the photosynthetic bacteria Rhodoschudomonas lutira, the immunity and disease resistance can be further increased to improve the productivity of cultured fish.

Hereinafter, the fish feed additive and feed using the photosynthetic bacteria of the present invention will be described in detail.

The feed additive of the present invention includes photosynthetic bacteria Rhodoschudomonas rutira and water.

Photosynthetic bacteria used in the present invention is a known microorganism, Rodopsudomonas rutila .

It will be described in detail the process of obtaining the feed additive and the photosynthetic bacteria used in the feed of the present invention Rhodoshudomonas rutira.

First, water is collected at the point where seawater and river meet and suspended in a minimal liquid medium, followed by primary culture under conditions that allow photosynthetic growth, that is, under anoxic conditions where light of 20 to 30 ° C. is irradiated. . In this case, in order to effectively grow photosynthetic bacteria, nutrients such as substrate and yeast extract may be added to the medium.

The primary culture solution is subjected to second culture for 2 to 3 days using a smear plate method or an injection plate method to separate reddish colonies (colony) to obtain photosynthetic bacteria Rhodoshu domonas lutira.

In this case, when several photosynthetic bacteria are present in the medium, various types of colonies are formed. As shown in FIG. 1, only the cells in which rod shapes of less than 1 μm are bonded to each other through a phase contrast microscope are used. Obtains Colonies of Pseudomonas Lutira.

Rodoschudomonas luti is a type of purple non-sulfur bacteria that has a Gram negative response and performs flagellar movement. The shape is simple and forms many clusters, and the cells are strongly bonded to each other.

Rhodoshudomonas rutira is obtained by separating a single colony of Rhodoshudomonas rutira in the same manner as described above.

The components of the optimal medium for mass cultivation of isolated Rhodoschudomonas rutira are as follows.

Medium composition consisted of Yeast extract 1.00 ~ 2.00g, Ethanol 0.50 ~ 1.00ml, Na ₂ -succinate 1.00 ~ 2.00g, Fe (III) citrate solution (0.5% in H ₂ O) 1.00 ~ 2.00ml, KH ₂ PO ₄ 0.10 ~ 1.00g, MgSO ₄ x 7 H ₂ O 0.10 ~ 1.00g, NaCl 0.10 ~ 1.00g, NH ₄ Cl 0.10 ~ 1.00g, CaCl ₂ x 2 H ₂ O 0.10 ~ 1.00g, Vitamin B12 solution (10 mg in 100 ml H ₂ O) 0.10 to 1.00 ml was added. Wet sterilization (121 ° C, 1.2 atm, 15 minutes) allows for easier extraction of colony cells.

In order to reduce the cost of the medium, the inorganic nutrient component of the above-mentioned medium composition may be coped with by adding 0.10 to 1.00 g of mineral. The mineral uses minerals such as biotite.

Feed the medium according to the embodiment of the present invention by injecting 0.5 to 1.5L of the medium into 17L of water and inoculating 1 to 3L of Rhodoschudomonas rutira and filling it in a transparent plastic pack under light irradiation at 25 to 30 ° C. under anaerobic conditions. An additive is prepared.

If necessary, a further 4-5th subculture can be used to obtain high concentrations of Rodoschudomonas rutira.

The number of cells of Rhodoshu domonas lutira obtained during the culture under the conditions described above is 6 to 3 × 10 ⁹ cells / ml.

As shown in Table 1 below, the Rodoshudomonas ruti cultivated by the above method has a significantly higher protein content than other protein sources such as chlorella.

[Table 1] General composition

division Crude Protein (%) Crude fat (%) Soluble Sugar (%) Crude fiber (%) Ash content (%) Ruthira 59.25 7.64 23.24 2.75 4.21 Chlorella 53.76 6.31 19.28 10.33 1.52 rice 7.48 0.94 90.60 0.35 0.72 bean 38.99 19.33 30.93 5.11 5.68

It also contains essential amino acids of good quality, as shown in Table 2 below.

[Table 2] Amino Acid Composition

division Rutira (g / 100g drying) Chlorella (g / 100g drying) Yeast (during g / 100g drying) Histidine 1.38 1.06 0.90 Arginine 3.31 3.24 2.50 Throneine 2.73 2.28 2.65 Proline 2.75 2.12 1.77 Glycine 2.40 2.28 2.18 Alanine 4.68 2.98 2.86 Valine 3.36 3.02 3.20 Methionine 1.42 0.27 0.51 Isoleucine 2.71 2.44 2.63 Leucine 4.63 4.46 3.54 Tyrosine 1.74 0.96 1.30 Tryptophaan 1.12 0.64 0.66

And the vitamin content of Lutira is shown in Table 3 below.

[Table 3] Vitamin Content

vitamin  Rhodoshudomonas rutira (㎍ / 100g)  Yeast (µg / 100g) B ₂ 3,800 2,900 B ₆ 3,400 2,400 Folic acids 2,000 1,700 B ₁₂ 400 One C 20,000 - D 10,000 IU 300,000 IU E 31200 -

In addition, as shown in Tables 1 to 3 above, a variety of vitamin C, including vitamin B group, is rich in amino acids such as lysine, histidine, and arginine, which are rich in protein and are easily lacked in fish grown only with artificial feed. Abundant amounts of D, E provide balanced nutrition to fish.

In addition to the above, Rhodoshudomonas rutira contains abundant physiologically active substances such as bacteriochlorophyll, carotenoids, ubiquinones, menaquinones, and the like. Its color is vivid and its anti-microbial resistance to various bacterial and viral diseases.

In addition, the cell walls and membranes of Rhodoshu domonas lutira contain soft mucus polysaccharides that are highly elastic, unlike algae such as chlorella. This indicates that fish have a high digestive absorption rate when prey, and thus have high value as an initial feed of fry.

The feed additive according to another embodiment of the present invention includes 1 L of medium, 2 L of Rhodoshu domonas lutira, and minerals with respect to 17 L of water.

Minerals are inorganic trace elements such as manganese, iron, copper, zinc, calcium, iodine, cobalt and selenium, which are essential nutrients for growing healthy fish.

Minerals are very small, but they form a skeleton, regulate the osmotic pressure in the body, maintain the acid and base balance of the body fluids, and are involved in a variety of roles such as activator or component of the enzyme itself in the enzyme system.

As the mineral material, one or more selected from silicate clay mineral powders such as zeolite, bentonite, biotite, and pegmatite, or feldspars such as ganite, biotite and feldspar Use it.

The mineral material is used to pulverize the 200-300 mesh particle size to a high-temperature drying at 200 to 250 ℃ that there is no foreign matter.

Minerals are mixed by adding 5 to 25 g per 17 liters of water. If it is less than 5 g, the effect of adding minerals is insignificant, and if it exceeds 25 g, the meat texture is reduced.

In addition, the water used in the feed additives may not be added to the mineral when using mineral-rich groundwater. In this case, the mineral is preferably less than 25g in 17L of water.

The feed additive according to another embodiment of the present invention includes water, a medium, a Rodo Pseudomonas lutira, a mineral, and an organic germanium mixed blood meal.

Germanium is organic germanium (Ge-132, (GeCH ₂ CH ₂ COOH) ₂ O ₃ ) And inorganic germanium (GeO / GeO ₂ ), which must be organic germanium in combination with organic compounds in order for inorganic germanium to be absorbed into the body to be a beneficial component.

Organic germanium, which is not toxic and has a high absorption rate in fish, can contribute to antioxidant activity, degradation of waste materials, improvement of immune function, and disease prevention. In addition, it plays a role of promoting cell activation, nutrient binding, and supply. It is combined with impurities of inorganic substances or heavy metals in the body and discharged into urine within 24 to 48 hours.

Organic germanium can be ingested by fish, and inorganic germanium can be fed to the soil or fed with foliar fertilization. As it is extremely small, it is hard to expect the effect.

Therefore, the organic germanium contained in the feed additive according to another embodiment of the present invention uses blood powder made from a livestock in which germanium is fed.

As a method of making blood containing organic germanium, the germanium ore is ground and mixed with the feed. Germanium ore varies depending on where and when it is collected, but consists of approximately 50 or more components. The main component is SiO ₂ like other minerals, and is composed of other Al ₂ O ₃ , Fe ₂ O ₃ , CaO, MgO, K ₂ O, Na ₂ O and the like.

Germanium ore is ground and agitated with livestock feed in powder form. The mixing ratio is such that 0.6 to 0.8 parts by weight of inorganic germanium, particularly preferably 0.7 parts by weight, based on 100 parts by weight of the whole animal feed. Inorganic germanium ore powders and agitated animal feed powders can be fed to livestock as a powder or processed into pellets. In addition, leachate in which germanium ore or germanium ore powder is immersed in water can be fed together with the germanium mixed feed.

Inorganic germanium in feed is organically combined with organic compounds in the body of cattle and deer. In order to collect blood containing organicized germanium, it is desirable to collect the germanium-mixed feed within 24 hours from the time of final feeding. This is because germanium is absorbed in the body and more than 90% of germanium is released out of the body after 24 hours.

The collected blood is heated and coagulated to remove moisture with a press and then dried to form a powdered blood powder. Of course, other blood powder production methods may be used.

Blood meal is added to feed additives containing water, media, Rhodoschudomonas lutira, and minerals. In this case, 50 to 100 g, preferably 75 g is added to 20 L of the feed additive and mixed. Blood powder has the effect of being used as a protein source of not only organic germanium but also high protein.

The amount of organo germanium in blood powder produced by the above method was analyzed by ICP test. Inorganic germanium powder was also analyzed for foliar fertilizers and the results are shown in Table 4 below.

Table 4

 division  Organic germanium content (ppm / g) small 0.085 pig 0.081 cabbage 0.021 radish 0.023 Lettuce 0.026

It can be seen from the results of Table 4 that the use of livestock is much higher than that of vegetables using organic germanium.

As described above, the feed additive of the present invention uses only photosynthetic bacteria or adds an active ingredient of minerals and organic germanium to another embodiment, and thus is more effective for immunization and growth of fish.

On the other hand, the fish feed according to an embodiment of the present invention includes the feed additive. Preferably, 250 ml of feed additive is added to 20 kg of feed.

And the feed of another embodiment of the present invention includes a protein source, carbohydrate source and lipid source with the feed additive.

As a protein source, any one or more selected from fish meal, casein, meat bone meal and shrimp powder is used. As the carbohydrate source, any one or more selected from sweet potato starch, potato starch and wheat starch is used. As a lipid source, any one or more selected from rapeseed oil, soybean oil, corn oil, pollock liver oil, and squid liver oil are used.

In addition, vitamin mixtures, caking additives, supplements and the like may be further included.

And feed additives of the present invention can be used in addition to conventional feeds such as feed or rice straw, grass, wild grass, soybean pods.

The feed comprising the protein source, carbohydrate source, and lipid source together with the feed additive of the present invention is economical because it contains sufficient essential nutrients and lowers the production cost of the feed.

Hereinafter, the content of the present invention will be described in detail through examples and test examples. This is for explaining the present invention in more detail, and the scope of the present invention is not limited to the following examples.

Example 1

17 L of water was mixed with 1 L of a medium composed of the following components for culturing Rhodoshu domonas lutira.

The composition of the medium was 1.50g of yeast extract, 1.50g of ethanol, 0.75ml of ethanol, 1.50g of Na ₂ -succinate, 1.50ml of Fe (III) citrate solution (0.5% in H ₂ O), 0.55g of KH ₂ PO ₄ , MgSO ₄ x 7 0.55 g H ₂ O, 0.55 g NaCl, 0.55 g NH ₄ Cl, 0.55 g CaCl ₂ × 2 H ₂ O, 0.55 mL Vitamin B12 solution (10 mg in 100 ml H ₂ O) was added.

And 2 L of Rhodoshu domonas rutira was inoculated in the mixture of water and the medium, and filled in a 20 L transparent culture pack, and irradiated with light under anaerobic conditions at a temperature of 25 ° C. to prepare a 20 L feed additive.

The number of RhodoSudomonas rutira cells in the feed additive was 5 × 10 ⁹ cells / ml.

Example 2

Prepared in the same manner as in Example 1, but was further mixed with 15g biotite powder as a mineral to prepare a feed additive.

Example 3

Prepared in the same manner as in Example 1, but further mixed 75g of the blood powder using the blood taken from the cow with respect to 17L of water.

The cows were fed a diet containing 0.7 parts by weight of germanium powder for 30 days in order to obtain blood. At this time, the germanium powder was used as a gemstone containing 0.5ppm inorganic germanium per 1g of gemstones. The feed of germanium-containing feed daily provided the usual daily feed required for raising deer. Two hours after feeding the final feed, blood was collected from the cow's blood.

1. Experimental Example 1: Water Purification Ability

Putrescine, cadaverine, hydrogen sulfide, mercaptan (merkaptan), which are the causative agents of odor generated during proteolysis, to investigate the water purification ability of photosynthetic bacteria Rhodoschudomonas lutira used in the present invention ) Was tested. The results are shown in Tables 6 and 7 below. The experimental group of Table 5 and Table 6 was taken 100ml of the water tank in the flounder cultured was added 0.001ml feed additive of Example 1 and the control was not added.

Table 5 Removal of putrescine, cadaverine, and hydrogen sulfide after 7 days

 division Control (mg / 100ml) Experimental zone (mg / 100ml) dark light dark light Putrescine 5.0 5.0 1.8 0.1 Cadaverine 5.0 5.0 2.1 0.1 Hydrogen sulfide 5.0 5.0 3.8 0.8

Table 6 Removal of mercaptan after 4 days

division Control (ppm) Experimental zone (ppm) CH ₃ SH 1.000 One CH ₃ SCH ₃ 800 0 (CH ₃ ) ₂ S ₂ 3.400 3

From the results of Table 5 and Table 6 it can be seen that in the experimental group administered a photosynthetic bacterium Rhodoschudomonas lutira to the causative agent of odor is significantly reduced compared to the control group.

In addition, it can be seen that the effect on the odor-removing ability of Rhodoschudomonas lutira is much increased in a light environment than in a dark environment. The feed additive of the present invention using Rhodoshu domonas lutira photosynthetic bacteria has a significant effect on improving the water quality of the aquaculture environment as in the above results.

2. Experimental Example 2: Antibacterial Activity

The antimicrobial activity of Rhodoshudomonas rutira used in the feed additive of the present invention was examined.

E. coli 0-157 and Salmonella enteritidis were used as strains to measure antimicrobial activity.

After inoculating the strains in saline, experimental groups were added with 1% aqueous solution of the feed additive of Example 1 and incubated for 24 hours, and then plated in selective medium. After culturing for 48 hours after the smear, the number of individuals of each strain was measured. The control group was not added to the feed additive solution.

The results are shown in Table 7 below.

Table 7

Strain division  After 48 hours (cells / ml) % Reduction Salmonella (SE) Control 4580 ± 120 - Experiment 1568 ± 85 65.8 O-157 Control 4825 ± 100 - Experiment 1450 ± 73 70

As can be seen from the results of Table 7, it can be seen that the rodoshudomonas lutira used in the feed additive and feed of the present invention effectively inhibits the growth of pathogenic bacteria, and thus has an excellent antibacterial effect.

3. Experimental Example 3 Fish Growth and Mortality Test

In order to investigate the efficacy of the feed additive of the present invention, the feed and the mortality test of the fish were carried out by administering the feed to which 250 ml of the feed additive of Examples 1 to 3 was added to 20 kg of the general compound feed for 30 days.

The fish species were divided into three groups of 8g, 10g and 15g in weight, and each group was treated with the feed additives of Examples 1, 2, and 3, respectively. Experiment 2 and Experiment 3, divided into a control group administered with the general formula feed without addition of the feed additive of the present invention.

In the rearing of farmed fish, the health status of the larvae, growth control and feeding of the feed at the larval stage are essential for the breeding of high quality farmed fish. The results for 30 days of form are shown in Table 9 below.

The water temperature was maintained at 25 ° C. for the experiment, and 3000 animals were stocked in 1 ton water tanks.

Table 8 Growth and Mortality

division  Average weight (g) Average body length (cm) % Mortality   5g group Experiment Zone 1 19.5 13.2 0 Experimental Zone 2 19.4 13.2 0 Experiment Zone 3 19.7 13.4 0 Control 13.1 9.8 76   10g group Experiment Zone 1 23.8 14.9 0 Experiment Zone 2 23.9 14.8 0 Experiment Zone 3 23.9 14.9 0 Control 17.6 12.2 71   15g group Experiment Zone 1 28.5 17.1 0 Experiment Zone 2 28.5 17.3 0 Experiment Zone 3 28.7 17.5 0 Control 20.3 13.3 64

As shown in Table 8, the addition effect of the feed additives on the average body weight and body length of the flounder fry did not show a significant difference in Experiments 1 to 3, but the results of Experiment 3 was greater than Experiment 1 and Experiment 2 Somewhat good.

And when comparing the control group without the feed additive of the present invention and the experimental groups 1 to 3, the difference was remarkable, which was effective in nutrients such as proteins and essential amino acids contained in photosynthetic bacteria Rhodoshudomonas lutira The result is.

The mortality rate, one of the most important management items in the cultivation stage of fry, shows that all of the experimental groups 1 to 3 containing photosynthetic bacterium Rhodoschudomonas lutira are effective in suppressing mortality.

4. Experimental Example: Fish weight and mortality according to aquaculture density

In order to examine the possibility of mass densities using the feed containing the feed additive of the present invention, 1500 and halibut fish weighing 5 grams were stocked in a 1 ton tank, respectively, and the feed additive according to Experimental Example 1 as feed. It was grown for 30 days by dividing the experimental group into which the feed was added and the control group into which the feed without the feed additive of the present invention was added.

The results are shown in Table 9 below.

Table 9

division Average weight Average length % Mortality 1500 groups Experiment 20.3 13.9 0 Control 15.9 10.7 71 6000 horses Experiment 20.8 13.6 0.5 Control 15.6 9.2 84

From the results of Table 9, both groups showed higher growth rate in body weight and body length than the control group. All flounders showed bright ocher body color in the experimental group, but the control group showed dropout or blackening of the body scales, which was more severe in the 6000 group than in the 1500 group.

In the experimental group, no fish died until the last day in the 1500 group and 0.5% in the 6000 group. However, the mortality rate of control group was 71% and 84%, which was significantly higher than that of the experimental group, especially in the dense 6000 group.

As a result of bacteriological examination of the dead fish species, Vibrio sp. Was isolated from most of the control. However, the experimental group did not show Vibrio bacteria in dead fish species.

From the results, it can be seen that the feed mixed with the feed additive of the present invention has higher resistance to pathogens than the general feed, and the growth rate is also good. In particular, even in large-scale densities, water purification and antimicrobial properties of Rhodoshu domonas lutira are effective.

5. Experimental Example: Quality Analysis of Olive Flounder

The general components, constituent amino acids, fatty acids, and physical properties of the flounder cultured by feeding the feed additive of the present invention to the flounder were added, and the results are shown in Tables 6 to 9.

The experimental group fed the feed to which the feed additive of Example 1 was added, and the control group fed the general feed. The results are shown in Tables 10-13.

Table 10 Analysis of General Ingredients

division Moisture (% / 100g) Fat (% / 100g) Ash content (% / 100g) Protein (% / 100g) Carbohydrate (% / 100g) Experiment 76.168 1.418 0.515 21.900 0

In Table 10, the fat, ash, and protein of the components of the flounder fed the feed to which the feed additive of the present invention is added were good.

[Table 11] Constituent amino acids

Amino Acid (mg / 100g) Control Experiment Asp 2460.360 2660.063 Thr 117.379 20.670 Ser 699.404 778.213 Glu 4350.093 4577.884 Pro 202.783 94.031 Gly 964.931 1003.532 Ala 933.494 932.746 Cys 0.000 0.000 Val 737.174 945.126 Met 594.719 741.427 Lie 912.903 1122.718 Leu 3187.005 3545.129 Tyr 821.969 1056.324 Phe 1008.100 1254.867 His 682.430 820.185 Lys 1885.139 1987.706 Amm 612.567 728.020 Arg 1693.424 2031.468 Total 21863.874 24300.1009

In order to analyze the constituent amino acid, 0.5 g of a sample was weighed into an 18 ml test tube, 3 ml of 6 N HCl was added, and the test tube was sealed using a vacuum pump.

The sealed test tube is hydrolyzed for 24 hours in a heatting block set at 121 ℃. After the hydrolysis was completed, the sample was removed with a rotary evaporater at 50 ° C. and 40 psi. After 10 ml of sodium loading buffer, 1 ml of the sample was filtered through a membrane filter of 0.25 ul and quantitated by an amino acid analyzer.

The results are shown in Table 12. In the case of flounder fed feed additive of the present invention, it can be seen that the amino acid composition is higher than the control group as a whole.

Feeding these feed additives gives an important meaning of increasing essential amino acid content.

Table 12 Fatty Acid Composition

Fatty acid (wt%) Experiment Control Butyric Acid Methyl Ester (C4: 0) 0 0 Caproic Acid Methyl Ester (C6: 0) 0 0 Capric acid (C10: 0) 0 0 Undecanoic acid (C11: 0) 0 0 Lauric acid (C12: 0) 0 0 Tridecanoic acid (C13: 0) 0 0 Myristic acid (C14: 0)) 4.323 5.100 Pentadecanoic acid (C15: 0) 0 0 Palmitic acid (C16: 0) 27.864 32.448 Heptadecanoic acid (C17: 0) 0 0 Stearic acid (C18: 0) 7.154 9.040 Arachidic acid (C20: 0) 0 0 Heneicosanoic acid (C21: 0) 0 0 Behenic acid (C22: 0) 0 0 Tricosanoic acid (C23: 0) 3.097 1.297 Lignoceric acid (C24: 0) 0 0 Myristoleic acid (C14: 1) 0 0 cis-10-Pentadecenoic acid (C15: 1) 0 0 Palmitoleic acid (C16: 1) 4.650 7.229 cis-10-Heptadecenoic acid (C17: 1) 0 0 Elaidic acid (C18: 1n9t) 0 0 Oleic acid (C18: 1n9c) 19.215 18.090 cis-11-Eicosenoic acid (C20: 1) 0.353 0.379 Erucic acid (C22: 1n9) 0 0 Nervonic acid (C24: 1) 0 0 Linolelaidic acid (C18: 2n6t) 0 0 Linoleic acid (C18: 2n6c) 1.538 1.630 cis-11,14-Eicosadienoic acid (C20: 2) 0.296 0.340 cis-13.16-Docosadienoic acid (C22: 2) 0 0 r-Linolenic acid (C18: 3n6) 0 0 Linolenic acid (C18: 3n3) 0 0 cis-8,11,14-Eicosatrienoicacid (C20: 3n6) 0 0 cis-11,14-Eicosatienoic acid (C20: 3n3) 0 0 Arachidonic acid (C20: 4n6) 0 0 EPA (C20: 5n3) 6.046 4.759 DHA (C22: 6n3) 25.464 19.113

In order to analyze the fatty acid, 5g of the sample is collected in a beaker and homogenized with a waring blander. 10ml chloroform and 20ml methanol were added thereto, homogenized again for about 2 minutes, and 10ml chloroform solution was added and sprayed for about 30 seconds. Watman No. 1 Filter using filter paper, transfer the filtrate to a 50 ml measuring cylinder, wait until the layers separate for about 30 minutes, remove the upper layer (aqueous layer) when the two layers are separated, and add a little Na ₂ CO ₄ reagent to the lower layer. After removing water completely, chloroform in the sample was completely removed using a rotary evaporator. If only fatty components remained, it was dissolved in 5ml toluene and analyzed by GC after methylation treatment.

Fatty acid analysis of Table 12 shows a higher tendency of the polyunsaturated fatty acid in the experimental group compared to the control. This is effective in preventing various cardiovascular diseases and lowering blood cholesterol in the case of ingesting the meat of fish, which is fed by feeding the feed with the feed additive of the present invention, to help prevent adult diseases.

[Table 13] Measurement of Properties

Viscosity speed shear stress shear rate temperature time interval Control Experiment 0.00 0.00 0.00 0.00 0.00 10.00 00: 30.2 433400.00 434700.00 5.00 0.00 0.00 10.00 00: 30.2 216700.00 217950.00 10.00 0.00 0.00 10.00 00: 30.2 144466.67 145300.00 15.00 0.00 0.00 10.00 00: 30.2 108350.00 108975.00 20.00 0.00 0.00 10.00 00: 30.2 81680.00 87180.00 25.00 0.00 0.00 10.00 00: 30.2 72233.33 72650.00 30.00 0.00 0.00 10.00 00: 30.2 57757.14 62271.43 35.00 0.00 0.00 10.00 00: 30.2 52600.00 54487.50 40.00 0.00 0.00 10.00 00: 30.2 48155.56 48433.33 45.00 0.00 0.00 10.00 00: 30.2

Viscosity measurement conditions are as follows.

Model: HADA-III + RHEOMETER (Brookfield)

-ADAPTER: Small Sample ADAPTER

-Speed: 0,5, 10, 15, 20, 25, 30, 35, 40, 45

Temperature: 4 ℃

Intervel time: 30 sec

-SPINDLE: HA / HB SPINDLE 3

The flounder feeding the feed containing the feed additive of the present invention from the results of Table 13 is excellent in viscosity and good chewability by using Rhodoshudomonas rutira to produce high quality fish with good viscosity at the time of fish farming. It can be seen that.

As described above, the present invention has been described with reference to one embodiment shown in the drawings, which is merely exemplary, and it will be understood by those skilled in the art that various modifications and equivalent embodiments are possible. .

Therefore, the true scope of protection of the present invention should be defined only by the appended claims.

1 is a phase contrast micrograph of Rhodoschudomonas rutira.

Claims (5)

A feed additive for fish using photosynthetic bacteria, comprising photosynthetic bacteria Rhodoshudomonas lutira and water. The fish feed additive for fish using photosynthetic bacteria, characterized in that the water further comprises a mineral. [Claim 3] The feed additive for fish using photosynthetic bacteria according to claim 2, wherein the water further comprises blood powder containing organicized germanium. Fish feed comprising the feed additive of any one of claims 1 to 3. The method according to claim 4, in addition to the feed additive, At least one protein source selected from fish meal, casein, meat bone meal, shrimp powder, Any one or more carbohydrate sources selected from sweet potato starch, potato starch, wheat starch, Feed for fish, characterized in that it further comprises at least one lipid source selected from rapeseed oil, soybean oil, corn oil, pollock liver oil, squid liver oil.

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