KR100672692B1 - Feed utilizing the wormwood and its preparation method - Google Patents

Abstract

Provided are a method for preparing a feed, and the feed prepared by the method which inhibits the proliferation of harmful microorganism and controls the pH of intestine. The method comprises the steps of washing mugwort(Artemisia capillaris), removing water and naturally drying it; cutting it into a size of 1-1.5 cm; spraying a culture solution to the mugwort; anaerobic fermenting it at a temperature of 30 deg.C for 15 days; examining the generation of fungi and the number of microorganism; and packaging it by unit, wherein the culture solution comprises the microorganism comprising 10% of Lactobacillus acidophilus, 10% of Lactobacillus casei, 10% of Lactobacillus plantarum, 40% of S. cerevisiae, 20% of B. subtilis and 10% of Rhodopseudomonas capsulata; syrup; and water.

Description

Feed utilizing the wormwood wormwood and its preparation method {Feed utilizing the wormwood and its preparation method}

1 to 3 are photographs showing the culture results of the mixed microorganisms to examine the mixing ratio of the microorganism and molasses used in the present invention,

4 is a manufacturing process of the feed using the wormwood according to the present invention,

Figure 5 is a graph showing the volatile fatty acid content of the feed using wormwood according to the present invention.

The present invention relates to a feed using a wormwood and its manufacturing method, and more specifically, after washing 1-3 times in a washing plant to remove the reinforced lion foot fresh mugwort collected from about 60 ~ 80 ㎝ ultra long, natural shade method Dried to around 15% of water content, and then cut into 1 ~ 1.5cm, and as lactic acid bacteria Lactobacillus acidophilus 10%, Lactobacillus cathay ( L. casei ) 10%, Lactobacillus plaus L. platarum 10%. As yeast, microorganisms composed of 40% S. cerevisiae , 20% B. subtilis and 10% Rhodopseudomonas capsulata as photosynthetic bacteria were mixed with molasses and water. After incubating the culture solution incubated for 24 hours at 30 ℃ using a sprayer to spray evenly 50 ~ 60% by weight with respect to the dry mugwort corresponding to within 15% of water content, and the microorganism adhered mugwort at 30 ℃ for 15 days anaerobic After fermentation, the fragrance, mold formation, and total microbial count of the microorganism are 1 x 10 ⁷ ～ It relates to a method of producing a feed using wormwood and feed prepared by the above method, characterized in that the unit packaging after checking whether it is 10 ⁹ cfu / ml.

Mugwort is a perennial plant belonging to the Asteraceae family, and it is widely distributed in Korea, Japan, and China. Mugwort is known to be effective against dozens of diseases by pressing white bottle and controlling all instruments. Because of its taste, it has been used as a material for rice cakes and rice cakes such as songpyeon and slices. In particular, wormwood clears blood and helps digestion, and it is also effective for skin beauty. Attempts have been made to add to a variety of foods.

The unique scent of mugwort is due to the essential oil component of cineol, which stimulates the secretion of digestive juices to help digestion after meals, and the bitter taste of bittersweet is a glutinous ingredient.

In particular, Ganghwa lion foot wormwood grows in the foot of 100m above sea level of Ganghwa Beach, which is blessed with oily land, sea breeze and sea breeze, and the effect of Ganghwa lion foot wormwood is described in the Dangun myths of Samguk Yusa. Mugwort ”, Heo Joon's motion contains nutrients (outpost-carotene, leaf-vitamin A, B, C, root-Artesmos) with different effects on leaves, roots, and stems as originated in various women's diseases. It is recognized as one of the best in the world of oriental medicine, and it is recorded as a special product of Ganghwa Island in Dongguk Yeoji Seungram and Herbology. It is famous for three major wormwoods, such as Ganghwa Gilsan Mountain Lion Foot Wormwood, Manisan Lion Foot Artemisia, and Haeyang Mountain Lion Foot Artemisia.

Mugwort is not poisonous, it has been known to ward off the bad energy and customs of the five intestines, and it is good to use it as a folk remedy for colds as well as to bleeding or bleeding. In addition to the wormwood has been used a lot as a hemostatic drug such as nosebleeds, uterine bleeding in oriental medicine, it is effective in digestion, pain in the lower abdomen, elimination of hookworms and odors, and is effective in gastrointestinal diseases, constipation, neuralgia, cold sickness, women's diseases and asthma It has been drinking as a mugwort tea.

Mugwort is rich in amino acids such as threonine, tryptophan, valine, alanine, and leucine, which can be used as food resources.Alkali is the main ingredient. , Essential oils, various minerals, vitamins, etc. are known, the main nutritional ingredients of mugwort are 5.2g protein, carbohydrate 10.6g, calcium 93mg, phosphorus 55mg, iron 10.9mg, vitamin A 7940I.U. Vitamin C is 20mg, which is rich in minerals and vitamins. Vitamin A is known to contain twice as much carrot as it is.

Calories (Kcal) moisture (%) Protein (g) Lipid (g) Carbohydrate (g) Fiber (g) Ash (g) Ca (mg) Fe (mg) Vt.A (I.U) Vt.B (mg) Vt.C (mg) 56 81.4 5.2 0.8 4.0 3.7 2.0 93 10.9 7940 0.44 20

Essential oil is a mixture containing a large number of fragrance substances in a certain ratio, a single component has a low pharmacological effect, but a mixture of more than 65 kinds of compounds showing great efficacy by mutual inhibition and edification of several components, lion foot mugwort The essential oils contained in it are cineol, tricyclene, bornol, borneol, thujone, terpnene, terpine, etc. Aureus ( Staphylococcus aureus), a Streptococcus Sangu devices cause of dental caries (Streptococcus sanguis ), Streptococcus mutans (S. mutans), Escherichia coli (E. coli), In recent years, many studies have shown that there is an antimicrobial effect of inhibiting pathogenic microorganisms such as Salmonella , as well as pharmacological characteristics such as increased immunity, nervous system stability, anticancer effect, anti-aging and antibacterial activity against skin diseases. It has been.

Artemisia Capillarisin and 6,7-dimethylesculetin contained in capillaris have the effect of promoting the secretion of bile acids, lipolytic enzymes secreted by the liver, and in the dimethyl contained in buds. Sureretin has been shown to protect the liver, and water-soluble extracts have been reported to improve glucose metabolism.

Mugwort extracts were found to have vasorelaxant effects on blood pressure using rats and rabbits, and the effects of mugwort, dermis, and larvae on lipid metabolism in rats with hyperlipidemia induced by high fat diet. It was known that the total lipid concentration in blood showed a tendency to decrease in mugwort and dermis treatments compared to the control and larvae treatments. Also, Artemisia iwayomogi Kitamura ) The effects of ethanol extracts on rat liver, serum cholesterol and lipid metabolism decreased serum cholesterol and triglycerides, suggesting that wormwood extract promotes lipid metabolism in liver. The extract has the effect of activating the tumor necrosis factor, and has been reported to have an anti-tumor effect by direct cytotoxicity, and in the experiments using rats, cineol is known to have a chemotherapy effect on breast cancer.

Mugwort having a variety of efficacy as described above is used only for a specific use, it is not used in large quantities is the situation that the native wormwood is left unattended.

In order to use it as a feed, feed intake increased when 10% of the feed was replaced with dry mugwort in the dried mugwort feeding test for the castrated cattle, and 5% and 10% of the feed was replaced with dried mugwort. The increase in body weight was improved, and metabolic studies on sheep were reported to improve dry matter, crude protein, and crude fat digestibility.

In addition, it was reported that the increase in weight gain, feed intake, and intestinal beneficial bacteria Bifidobacteria were increased, but harmful bacteria Clostridium and Escherichia coli were decreased when 5% of worm extracts were administered to rats. There was no difference in weight gain and dietary intake in dried mugwort feeding trials. However, replacing 3% of dietary supplements with dry mugwort reduced feed intake and improved feed demand, but did not affect meat color. In addition, there was also a study showing that the content of docosanoenoic acid (DHA), which is a ω3 fatty acid, increased in the flesh, and the feed value of wormwood was highly evaluated.

In addition, the replacement of dry mugwort in cotton sheep improved the crude protein digestibility and fat digestion rate compared to the control, and the mugwort was replaced with mugwort pellets that replaced 3 ~ 10% of the rich feed. As a result of dietary supplementation, crude protein digestibility, crude fat ADF, crude ash NDF digestibility were higher than control, and it could contribute to nutritional value of feed by improving nutrient utilization rate, improving fermentation conditions in rumen and increasing microbial nitrogen content. It was.

In addition, Korean Patent No. 430403 (A cattle feed supplemented with wormwood and Hanwoo meat fed with its feed) has a control and dry mugwort supplemented with only plain feed in plain rice straw to investigate the effect on the fatty acid composition of Hanwoo beef. 5 and 10% of C ₁₈ fatty acids, if the fillet as in added benefit unsaturated fatty acid _{level: 3} (linolenic acid) was doubled 4.9 ~ 6.3 compared to the control with increasing added benefit ratio of the dried mugwort, C _{20: 4} (arachidonic acid) increased from 5% and 10% of dried mugwort to 3.68% to 3.76%, which was significantly higher than that of 3% of dried mugwort and control. Especially, n ₂₂ fatty acid C _{22 : 6} (docosahexaenoic acid; DHA) was not detected in the control group, but as the supplementation rate of dried mugwort increased, the ratio increased to 0.21 ~ 0.31%, which contains a lot of essential fatty acids C _{18 : 2} and C _{18 : 3} (4) because it is saturated In defense, C _{16 : 0} increases the blood cholesterol level, but C _{18 : 3} decreases the blood cholesterol level. The demand rate was improved by about 4.6 ~ 9.1% in the 3 ~ 5% feeding group, the longest muscle area and the carcass grade in the 5% group, and the crude protein content of the meat in the 5 ~ 10% group. The redness and redness were excellent in the nutrient digestibility and palatability in 3%, and 5% in the nutrient digestibility, and the increase in nitrogen accumulation rate, unsaturated fatty acid content and DHA content of meat was increased by adding dry mugwort.

However, the conventional methods are not only satisfactory absorption of nutrients in the stomach or intestine by using dried mugwort as it is, and it was difficult to expect other effects other than the efficacy of mugwort.

Accordingly, it is an object of the present invention to provide a feed using wormwood that can obtain not only the effect of the wormwood, but also the effect of inhibiting the growth of harmful bacteria by effective microorganisms, proper regulation of intestinal pH, activity of fibrinolytic bacteria and the like.

Another object of the present invention to provide a method for easily preparing a feed using the mugwort of the above object.

In order to achieve not only the above objects but also other objects that can be easily expressed, the present invention washes the reinforced lion's foot mugwort from about 60 to 80 cm in length one to three times in a washing station and removes water. Dried to around 15% of water content, and then cut into 1 ~ 1.5cm, and as lactic acid bacteria Lactobacillus acidophilus 10%, Lactobacillus cathay ( L. casei ) 10%, Lactobacillus plaus L. platarum 10%. As yeast, microorganisms composed of 40% S. cerevisiae , 20% B. subtilis and 10% Rhodopseudomonas capsulata as photosynthetic bacteria were mixed with molasses and water. After incubating the culture solution incubated for 24 hours at 30 ℃ using a sprayer to spray evenly 50 ~ 60% by weight with respect to the dry mugwort corresponding to within 15% of water content, and the microorganism adhered mugwort at 30 ℃ for 15 days anaerobic After fermentation, the fragrance, mold formation, and total microbial count of the microorganism are 1 x 10 ⁷ ～ 10 ⁹ cfu / ml is tested, and the unit is packaged to produce feed using wormwood, not only the effect of wormwood, but also the inhibition of growth of harmful bacteria by effective microorganisms, proper regulation of intestinal pH, activity of fibrinolytic bacteria, etc. The effect could be obtained.

The present invention is described in more detail as follows.

Method for producing a feed using lion foot wormwood according to the present invention after washing the reinforcement raw lion foot wormwood collected from about 60 ~ 80 ㎝ 1 ~ 3 times in the washing station and removing water, water content using a natural shade method 15 After drying in% and then cut into 1 ~ 1.5cm, Lactobacillus acidophilus ( Lactobacillus acidophilus ) 10%, Lactobacillus cathay ( L. casei ) 10%, Lactobacillus platarum ( L. platarum ) as lactic acid bacteria 10%. Microorganism consisting of 40% S. cerevisiae , 20% B. subtilis and 10% Rhodopseudomonas capsulata as photosynthetic bacteria After mixing with the incubator cultured at 30 ℃ for 24 hours to spray evenly spraying 50 ~ 60% by weight with respect to the dry lion foot wormwood corresponding to the moisture content within 15% using a sprayer, and the lion foot wormwood with microorganisms attached 30 ℃ After 15 days of anaerobic fermentation, the aroma, mold formation and total microbial count of the product were 1 x 10 ⁷ ～ After checking for 10 ⁹ cfu / ml, it is characterized by unit packaging.

In the present invention, the harvesting of the reinforced raw lion foot wormwood is collected at about 60 ~ 80cm tall, which is harvested at the height of 20 ~ 100cm to investigate the change in building digestion and energy content, it is harvested at 60 ~ 80cm This is because feed efficiency is the highest.

Also, if the soil is too low when harvesting lion foot wormwood or when soiled on the lion foot wormwood during handling, it may be affected by the other foreign matter contained in the soil during the fermentation process. Dry using a drying method such as freeze drying, natural drying, etc. However, in the present invention, using a low-cost natural drying method is dried to around 15% moisture content.

Then, dried dried lion foot wormwood is cut into about 1 to 1.5 cm. This is to induce a closed anaerobic effect tablet which is a safe fermentation process. If the particles to be fermented are large, the density decreases to generate a contact surface with air. As the contact surface with air increases, a local exhalation effect may occur, which may cause loss of beneficial nutrients. In addition, it is preferable to crush and mix the thick stem separately.

Separately, lactobacillus Lactobacillus acidophilus ( Lactobacillus acidophilus ) 10%, L. casei ( L. casei ) 10%, L. platarum ( L. platarum ) 10%. Microorganism consisting of 40% S. cerevisiae , 20% B. subtilis and 10% Rhodopseudomonas capsulata as photosynthetic bacteria as yeast After mixing with and incubating at 30 ℃ for 24 hours to obtain a culture. At this time, it is also preferable to mix 2% actinomycetes ( Streptomyces griceus ) with respect to the total weight of the microorganism.

Functions and effects of the microorganisms used in the present invention are as follows.

Yeasts are fungal cells that have a single cell type and proliferate by germination or division, and vary depending on the culture conditions, but generally have high protein, nucleic acid, and vitamin content and low fat content. In addition, beta glucan, a cell wall component of yeast, activates the immune system of various animals and plants, increases resistance to various pathogenic microorganisms, improves vaccine production efficiency, decomposes lactic acid produced in the rumen, and lactic acid. It improves the availability of fibrin by promoting the growth of used bacteria, raising the pH in the rumen and increasing the number of fibrinolytic bacteria.

Lactic acid bacteria are microorganisms that can effectively produce high quality fermentation and are widely distributed in the natural world such as mammalian digestive tract, various fermented foods and soil. Various organic acids produced by these lactic acid bacteria lower the pH to 4.2 or less to neutralize pathogenic bacteria, and restore the total balance of enterobacteriaceae to normal to prevent diarrhea and enteritis. Therefore, rapid lactic fermentation by the growth of lactic acid bacteria has the effect of improving fermentation quality and preventing aerobic decay, maintaining the normal bacterial flora in the rumen and suppressing pathogenic microorganisms, especially preventing enteritis and hari, and thus increasing animal feed rate and feed It has the effect of improving efficiency. Photosynthetic bacteria, such as higher plants, using light energy to perform carbon assimilation, there are about 200 types of non-sulfur bacteria, about 150 types of red sulfur bacteria, and about 200 kinds of green bacteria. These photosynthetic bacteria are Gram-negative bacteria, and 65% of the cell components are composed of proteins, contain high amounts of essential amino acids and vitamins, and also have the ability to use hydrogen sulfide and organic acids, which are the source of odors. It works.

Bacillus is an aerobic or aerobic anaerobic bacterium that is widely present in soil and aquatic environments. It is an industrially important strain used for the production of various enzymes, vitamins, antibiotics, and pesticides, and it is a polymer such as starch, protein, cellulose, fat and xylan. It rapidly solubilizes and decomposes substances and magnetizes malodorous substances.

Actinomycetes produce a variety of biologically active substances, including antibiotics. Especially, antibiotics suppress pathogens and create a good environment for other beneficial bacteria to live. Therefore, it is effective to enhance the activity of beneficial microorganisms and livestock immunity when used in feed.

The microorganism used in the present invention is Lactobacillus acidophilus KCTC 3164, L. casei KCTC 2180, L. platarum KCTC 3108 as a known strain. S. cerevisiae KCTC 7286, B. subtilis KCTC 3560, Rhodopseudomonas capsulata KCTC 2583 as photosynthetic bacteria, Streptomyces glisus as actinomycetes ) KCTC 1072 was used.

The mixing ratio of the microorganisms is not particularly limited to the above categories, and may be changed through repeated experiments according to the type of microorganism and the characteristics of the fiber compound feed to be obtained.

The ratio of mixing microorganisms with molasses and water is not particularly limited, but the ratio of microorganisms to molasses = 1: 1 to 0.2 and microorganisms to water = 1: 5 is preferable because the number of target microorganisms can be obtained. It was most preferable to mix molasses with water in the ratio of 1: 1: 1.

The microbial culture cultured as described above is sprayed using a sprayer while spraying 50 to 60% by weight relative to the dry lion foot weight of the wormwood corresponding to the moisture content within 15%. However, if it is not mixed evenly, mold may occur.

Put microorganism-coated lion foot wormwood in an airtight space (vinyl, barrel), remove the air as much as possible, seal it, and anaerobic fermentation for 15 days at a temperature of 30 ° C. If necessary, cultivate while controlling moisture by adding dryer or moisture control agent. Finally, the fragrance, mold formation, and total number of microorganisms (1 x 10 ⁷ ~) 10 ⁹ cfu / ml) is inspected and then packed in units to prepare the product according to the present invention.

The following Examples, Comparative Examples, and Experimental Examples illustrate the present invention in more detail, but do not limit the scope of the present invention.

Experimental Example  One

Since the culture environment of the mixed microorganism selected to ferment the lion foot wormwood affects the fermentation results, the following experiment was performed by varying the concentration of molasses to be fed to the microorganism to obtain an appropriate fermentation result.

5g, 2.5g, 1g of molasses in a 50ml tube (tube) was adjusted to 25ml in total with distilled water, and 5ml of mixed effective microorganisms were inoculated and incubated in a 30 ° C incubator for 24 hours. In order to determine the proper environment, the plate was plated in MRS medium, and after 24 hours, the total number of microorganisms and the number of digits of yeast were observed. The pH was also measured, the results are shown in Table 2, and the photographs of the culture results were taken. Shown in

As can be seen from Table 2 and FIGS. 1 to 3, the total number of microorganisms in each experimental group increased slightly as the concentration of molasses decreased. This is because the lactic acid bacteria having a relatively small cell size increased and there was no significant difference. The number of molasses increased as the concentration of molasses increased, and accordingly, the pH also increased.

In the case of fermented feed, the concentration ratio of microorganism and molasses was 1: 1 because pH and the number of yeast were also considered.

Experimental Example  2

In order to ferment by adding microorganisms to dried fermented lion foot wormwood, experiments were carried out as follows to determine whether there was a difference in the growth of fermented microorganisms according to the dried year and moisture content.

Lactobacillus acidophilus ( Lactobacillus acidophilus ) 10%, L. casei ( L. casei ) 10%, L. platarum (10%) as lactic acid bacteria. Microorganisms composed of 40% S. cerevisiae as yeast, 20% B. subtilis and 10% Rhodopseudomonas capsulata as photosynthetic bacteria and the total weight of the microorganisms The microorganisms mixed with 2% actinomycetes ( Streptomyces griceus ) and the additives (microorganism [1]: molasses [1]: water [5]) were mixed and cultured at 30 ° C. for 1 day. Let's do it. The total number of microorganisms after incubation is approximately 10 ⁷ ～ 10 ⁹ cfu / ml. The microbial culture solution was added to 5 kg of dried lion foot wormwood each prepared in an amount determined according to the experimental section below and fermented anaerobicly for 15 days in a 30 ° C. constant temperature room.

[Experiment 1] Drying (moisture content: 16.5%) 1 day + microbe 4000ml

[Experiment 2] Drying (Water Content: 12.3%) 1 Year Old + Microbe 3000ml

[Experiment 3] Drying (Moisture Content: 13.1%) 3 Year Old + Microbe 3000ml

     [Experiment 4] Drying (moisture content: 13.1%) 3 year old + microorganism 4000ml

As can be seen from Table 3, experiments 2 and 3 showed little difference in microbial growth according to dry age, and in case of lactic acid bacteria growth through [Experiment 3] and [Experiment 4]. You can see that this is important. In addition, in the case of [Experiment 1] and [Experiment 4], the growth of Bacillus showed a difference, which is thought to be more eluted material inhibiting the growth of Bacillus by long-term drying. In the case of actinomycetes growth, as in the basic investigation, all of the experimental groups were inhibited from growth.

Therefore, it can be seen that the growth of added microorganisms is affected by the water content rather than the dry age of lion foot wormwood.

Experimental Example  3

In the fermentation preparation of lion foot wormwood, experiments were carried out to determine the total moisture content, touch and smell according to the amount of microorganisms added to the dry lion foot wormwood.

5 kg of dried annual lion foot wormwood (moisture content: 12.3%) were added with 2000, 2500, 3000, and 4000 ml of microbial culture, respectively, and sealed in an anaerobic fermentation for 15 days at 30 ℃ constant temperature room. Odor was measured and evaluated and listed in Table 4.

As can be seen from Table 4, it is appropriate to add 50 to 60% of the microbial culture solution to the dry lion foot weight of the wormwood within 15% of water content, considering the appropriate moisture concentration, roughness and aroma of the stem. Judging.

Experimental Example  4

To determine the levels of volatile fatty acids of unfermented lion foot wormwood and fermented lion foot wormwood, volatile fatty acids of untreated fermented lion foot wormwood and fermented lion foot wormwood were measured. 5 and 5 are shown and shown.

Lactobacillus acidophilus ( Lactobacillus acidophilus ) 10%, L. casei ( L. casei ) 10%, L. platarum (10%) as lactic acid bacteria. Microorganisms composed of 40% S. cerevisiae as yeast, 20% B. subtilis and 10% Rhodopseudomonas capsulata as photosynthetic bacteria and the total weight of the microorganisms The microorganisms mixed with 2% actinomycetes ( Streptomyces griceus ) and the additives (microorganism [1]: molasses [1]: water [5]) were mixed and cultured at 30 ° C. for 1 day. Let's do it. The total number of microorganisms after incubation is approximately 10 ⁷ ～ 10 ⁹ cfu / ml. This microbial culture solution was added to 5 ml of 5 kg of dried lion foot wormwood, each prepared, and fermented anaerobicly for 15 days in a 30 ° C constant temperature room.

Volatile fatty acids are absorbed into the body of ruminants and supply 80% of the energy demand. Among them, iso - butyrate and iso- valerate are essential nutrients of fibrinolytic bacteria. .

The measured results showed that the total dry volatile fatty acid was higher in the fermented lion foot wormwood than in the untreated fermented lion foot wormwood, but the acetate was lower in the fermented lion foot wormwood than in the dry foot 3 year old. It is estimated that this is the difference in the fiber of lion cucurbita used in the experimental analysis, considering that acetate is produced when the fiber is fermented.

Experimental Example  5

After making 1 year old lion foot wormwood (water content: 12.3%), 5kg of microbial culture solution was added to 2500ml of microorganism culture solution, and then [Experiment 2] was prepared under anaerobic fermentation for 15 days in 30 ℃ constant temperature room. The organic acids of each of the experimental zones were measured and the results are shown in Table 6.

The ratio of lactic acid and acetic acid was good at about 7: 3, and in particular, the fermentation quality was excellent because the production of butyric acid was 0.25% and 0.08% of total organic acid, respectively.

In addition, the E. coli test was performed to evaluate the stability of the microbial fermented feed. As a result of the negative result in the qualitative test using the dry film method, the stability of the fermented feed was confirmed, and the growth of most microorganisms was inhibited at low pH conditions. Pathogenic microorganisms also do not seem to grow.

As described above, in the present invention, after washing the reinforcement lion foot wormwood collected in the ultra-long 60 ~ 80 ㎝ 1 ~ 3 times in the washing station and remove the water, and then dried to 15% moisture content using a natural shade method 1 to cut into 1.5㎝ and Lactobacillus know 10% filler's (Lactobacillus acidophilus) as a lactic acid bacterium, Lactobacillus Cathay (L. casei) 10%, Lactobacillus Plastic tareom (L. platarum) 10%. Microorganisms composed of 40% S. cerevisiae as yeast, 20% B. subtilis and 10% Rhodopseudomonas capsulata as photosynthetic bacteria and the total weight of the microorganisms Dried lion's foot wormwood that is within 15% of water content using a sprayer after incubating a microorganism mixed with 2% actinomycetes ( Streptomyces griceus ) with molasses and water for 24 hours at 30 ° C Mix 50 to 60% by weight with respect to spraying, and anaerobic fermentation of microorganism-adhered lion foot wormwood at 30 ° C. for 15 days, and then determine the fragrance, mold formation and total number of microorganisms (1 x 10 ⁷ ～) 10 ⁹ cfu / ml), and the unit packaging it was possible to obtain not only the effect of the wormwood but also the inhibition of growth of harmful bacteria by the effective microorganisms, the regulation of intestinal pH, the activity of fibrinolytic bacteria.

Claims (5)

Wash the collected lion foot wormwood 1-3 times in the washing station, remove the water, dry by using the natural shade method, cut into 1 ~ 1.5㎝, Lactobacillus acidophilus ( lactobacillus acidophilus ) 10 as lactic acid bacteria %, Cathay Lactobacillus (L. casei) 10%, Lactobacillus Plastic tareom (L. platarum) 10%. Microorganisms composed of 40% S. cerevisiae , 20% B. subtilis as yeast, 10% Rhodopseudomonas capsulata as photosynthetic bacteria, and the total weight of the microorganisms After mixing 2% actinomycetes with molasses and water, the culture medium incubated at 30 ° C. for 24 hours was sprayed onto the dried lion foot wormwood corresponding to about 15% water by using a sprayer and mixed evenly. The sticky lion foot wormwood was anaerobic fermented at 30 ° C. for 15 days, and then the fragrance, mold formation, and total microbial count of the product were 1 × 10 ⁷ ～. 10 ⁹ cfu / ml after checking whether the method of producing a feed using a lion foot wormwood, characterized in that the unit packaging. The method of claim 1, wherein the collection of lion foot wormwood is a method of producing a feed using the lion foot wormwood, characterized in that performed at a height of 60 to 80 cm. The method of claim 1, wherein the natural dryness of the washed lion foot raw wormwood is carried out with a water content of 15%. The method according to claim 1, wherein the mixing ratio of the microorganism and molasses is 1: 1 to 0.2, characterized in that the method for producing a feed using a lion foot wormwood. Feed using lion foot wormwood, characterized in that produced by the method of any one of claims 1 to 4.

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