KR101070236B1 - Method for increasing carnosine in muscle of chicken - Google Patents

Method for increasing carnosine in muscle of chicken Download PDF

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KR101070236B1
KR101070236B1 KR1020080090665A KR20080090665A KR101070236B1 KR 101070236 B1 KR101070236 B1 KR 101070236B1 KR 1020080090665 A KR1020080090665 A KR 1020080090665A KR 20080090665 A KR20080090665 A KR 20080090665A KR 101070236 B1 KR101070236 B1 KR 101070236B1
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carnosine
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중앙대학교 산학협력단
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    • A—HUMAN NECESSITIES
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    • A23K10/24—Animal feeding-stuffs from material of animal origin from blood
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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Abstract

본 발명은 계육 조직 내 카노신 함량의 증진방법에 관한 것으로, 보다 구체적으로는 광범위한 항산화능력을 가지는 것으로 알려진 카노신을 계육으로부터 얻기 위하여 혈분(blood meal)을 사료에 첨가하고 상기 사료를 육계에 공급하여서 계육 조직 내에서 카노신의 함량을 증진시키는 방법에 관한 것이다.The present invention relates to a method for enhancing the content of carnosine in poultry tissue, and more specifically, to obtain carnosine, known to have a wide range of antioxidant capacity, from a poultry by adding a blood meal to the feed and feeding the feed to broilers. A method of enhancing the content of cannosine in poultry tissue.

카노신은 항산화작용, 혈관확장작용, 효소조절 및 신경전달 물질 역할 등의 작용이 있는 것으로 알려진 디펩티드로, 카노신을 별도로 섭취하지 않고 본 발명의 방법으로 사양되어 공급되는 계육조직 내의 카노신을 음식물 형태로 자연스럽게 섭취할 수 있다. 또한, 본 발명의 방법에 따라 사양된 육계는 그 조직 내에 카노신 함량이 일반 육계보다 높으므로 기능성 축산품으로서 시장에서 경쟁력을 가지게 되어 양계 농가의 소득 증대원으로 제공될 수 있다.Cannosine is a dipeptide known to have antioxidant, vasodilating, enzyme regulating and neurotransmitter roles, and cannosine in poultry tissue, which is specified and supplied by the method of the present invention without food intake, in the form of food. You can eat it naturally. In addition, broilers specified in accordance with the method of the present invention has a higher content of carnosine in the tissues than ordinary broilers, so that it is competitive in the market as a functional livestock product and can be provided as a source of income for poultry farmers.

카노신, 안세린, 혈분, 사료, 계육 조직 Carnosine, anserine, blood meal, feed, poultry tissue

Description

계육 조직 내 카노신 함량의 증진방법{METHOD FOR INCREASING CARNOSINE IN MUSCLE OF CHICKEN}METHOD FOR INCREASING CARNOSINE IN MUSCLE OF CHICKEN}

본 발명은 계육 조직 내 카노신 함량의 증진방법에 관한 것으로, 보다 구체적으로는 광범위한 항산화능력을 가지는 것으로 알려진 카노신을 계육으로부터 얻기 위하여 혈분(blood meal)을 사료에 첨가하고 상기 사료를 육계에 공급하여서 계육 조직 내에서 카노신의 함량을 증진시키는 방법에 관한 것이다.The present invention relates to a method for enhancing the content of carnosine in poultry tissue, and more specifically, to obtain carnosine, known to have a wide range of antioxidant capacity, from a poultry by adding a blood meal to the feed and feeding the feed to broilers. A method of enhancing the content of cannosine in poultry tissue.

카노신 (Carnosine, β- alanyl -L- histidine)과 카노신으로부터 유도되는 안세린(Anserine, β- alanyl - methyl -L- histidine) (Boldyrev 등, 1990)은 포유동물의 근육 조직에서 발견되는, 각각 다음 화학식 1과 2로 표시되는 디펩티드(dipeptide)이다 (Gulewitsch 등, 1900; Ackermann 등, 1929; Tamaki 등, 1976).Kano Seen (Carnosine, β- alanyl -L- histidine) and anserine Kano Shin derived from (Anserine, β- alanyl - methyl -L- histidine) (Boldyrev et al., 1990) is found in mammalian muscle tissue, Dipeptides represented by the following formulas (1) and (2), respectively (Gulewitsch et al., 1900; Ackermann et al., 1929; Tamaki et al., 1976).

화학식 1Formula 1

Figure 112008065068928-pat00001
Figure 112008065068928-pat00001

화학식 2Formula 2

Figure 112008065068928-pat00002
Figure 112008065068928-pat00002

이러한 디펩티드는 그 효과에 대하여 많은 연구가 이루어졌으며, 대표적인 작용으로는 항산화 능력 (Boldyrev 등, 1987; Chan 등, 1995; Gopalakrishnan 등, 1999), 혈관확장 작용 (Ririe 등, 2000), 효소들의 조절 (Gianelli 등, 2000; Johnson 등, 1982; Johnson 와 Hammer, 1989; Parkers 와 Ring, 1970), 신경전달 물질의 역할 (Flancbaum 등, 1990; Ohtsuka 와 Takahashi, 1983) 등과 같은 신체 여러 분야의 대사도우미 기능을 하는 것으로 알려져 있다.These dipeptides have been studied for their effects, and their typical actions include antioxidant activity (Boldyrev et al., 1987; Chan et al., 1995; Gopalakrishnan et al., 1999), vasodilator action (Ririe et al., 2000), and regulation of enzymes. (Gianelli et al., 2000; Johnson et al., 1982; Johnson and Hammer, 1989; Parkers and Ring, 1970) and the role of neurotransmitters (Flancbaum et al., 1990; Ohtsuka and Takahashi, 1983). It is known to do.

디펩티드들 중 카노신은 최근 그 중요성이 날로 더해지고 있는데, 그 이유는 카노신의 광범위한 항산화 능력을 통하여 노화방지 효과와 외모를 젊게 유지할 수 있다(Quinn 등, 1992; Boldyrev 등, 1994)는 큰 이슈와 더불어 현대의학으로도 완치가 어려운 알츠하이머와 같은 뇌질환의 예방에 효과가 있다는 것이 알려졌기 때문이다.Among the dipeptides, cannosine has become increasingly important in recent years, because the antioxidative ability of cannosine can keep its anti-aging effect and appearance young (Quinn et al., 1992; Boldyrev et al., 1994). In addition, it is known that it is effective in preventing brain diseases such as Alzheimer's, which is difficult to cure even in modern medicine.

이와 같은 카노신의 효능은 글라이케이션(glycation)에 의한 유리기의 비정상적 세포공격 방어, 젖산 축적 방지, 중금속 이온의 킬레이터(Chelator) 작용 (Basic Health Publications User's Guide, Marie Moneysmith, Basic Health Publications, Inc. 2004), 유리기의 제거 (Chan. K 등, 1994) 등 다양하다.The efficacy of cannosine can be attributed to the defense of abnormal cell attack of free radicals by glycation, prevention of lactic acid accumulation, and chelator action of heavy metal ions (Basic Health Publications User's Guide, Marie Moneysmith, Basic Health Publications, Inc.). 2004), elimination of free radicals (Chan. K et al., 1994).

축산물의 품질과 가격경쟁이 치열한 현 상황에 더하여 FTA 체결에 따른 외국산 축산물의 무차별 공격이 멀지 않은 시점에서 기능성이 추가된 차별화된 축산제품은 가장 큰 경쟁력이며 이미 이러한 노력들이 여러 곳에서 이루어지고 있다. In addition to the current situation of fierce product quality and price competition, differentiated livestock products with added functionality are the most competitive and the efforts are already being made in various places when the invasion of foreign livestock products is not far from the FTA.

본 발명에서는 이러한 시대변화에 적응하기 위한 일환으로 항산화효과 등의 작용이 알려진 기능성 디펩티드인 카노신이 강화된 축산물의 생산방법을 연구하게 되었다.In the present invention, as a part of adapting to the changes of the times, a method for producing livestock products enriched with cannosine, which is a functional dipeptide known as an antioxidant effect, is known.

따라서 본 발명에서는 이에 착안하여 아미노산 조성 중 L-히스티딘 함량이 높은 혈분을 사용하여 카노신 강화 축산물을 개발하기 위하여 연구한 결과, 본 발명에 이르게 되었다.Therefore, in the present invention, the blood powder with high L-histidine content in the amino acid composition In order to develop a carnosine-enhanced livestock product, the present invention has been achieved.

본 발명의 목적은 계육 조직 내 카노신 함량의 증진방법을 제공하는 것이다.It is an object of the present invention to provide a method for enhancing the content of carnosine in poultry tissue.

본 발명의 다른 목적은 혈분을 포함하는 사료를 육계에게 공급하여 육계를 사양하여서 계육 조직 내의 카노신 함량을 증가시키는 방법을 제공하는 것이다.It is another object of the present invention to provide a method for increasing the content of carnosine in poultry tissue by feeding the broiler containing blood meal to the broiler.

또한, 본 발명의 목적은 카노신 함량이 증진된 계육을 제공하는 것이다.It is also an object of the present invention to provide poultry with enhanced cannosine content.

이하, 본 발명을 상세히 설명하면 다음과 같다.Hereinafter, the present invention will be described in detail.

본 발명은 사료의 총 중량에 대하여 혈분(blood meal) 5 내지 10중량%를 첨가하여 얻어진 사료를 육계에게 공급하여서 육계를 사양하여 이루어지는 것을 특징으로 하는 계육 조직 내 카노신 함량의 증진방법인 것이다.The present invention is a method for enhancing the content of cannosine in broiler tissue, characterized in that the broiler is characterized by feeding the feed obtained by adding 5 to 10% by weight of blood meal (blood meal) to the total weight of the feed.

이하, 본 발명을 더욱 상세히 설명하면 다음과 같다.Hereinafter, the present invention will be described in more detail.

본 발명에 있어서, “혈분’이란 동물의 혈액을 건조 및 분쇄시켜서 얻어지는 것으로, 통상적으로 도축장에서 나오는 혈액을 가열하여 응고된 것을 압착하여 탈수시킨 후 건조하여 분쇄해서 얻어지는 혈액의 건조분말을 의미한다. 본 발명에서 사용되는 혈분은 각종 동물, 예를 들면 소, 돼지, 닭 등의 혈액으로부터 얻어진 건조 분말을 사용할 수 있으며, 시판되고 있는 제품화된 형태의 혈분을 사용할 수도 있다.In the present invention, "blood powder" is obtained by drying and pulverizing animal blood, and generally means a dry powder of blood obtained by squeezing and dehydrating the dried coagulated blood by heating the blood from the slaughterhouse. As the blood powder used in the present invention, dry powders obtained from blood of various animals such as cows, pigs, and chickens can be used, and commercially available blood powders can also be used.

본 발명에 따르면, 육계의 사료 조성물에 사료 조성물의 전체 중량에 대하여 5 내지 10중량%의 혈분을 첨가할 수 있다. 이 때, 혈분의 첨가량이 10 중량 % 까지는 카노신의 증가 효과를 가져올 수 있지만 반대로 육계의 생산성 저하를 초래할 수 있다.According to the present invention, 5 to 10% by weight of blood meal may be added to the feed composition of broiler based on the total weight of the feed composition. At this time, the amount of blood added up to 10% by weight can bring an increase effect of carnosine, but on the contrary, it can cause a decrease in productivity of broilers.

본 발명에 있어서는, 육계의 사료 조성물에 혈분 이외에 추가로 마그네슘을 첨가할 수 있는데, 바람직하기로는 MgO 형태로 첨가한다. MgO는 사료 1 kg 당 약 1 g을 사용할 수 있다. 카노신 합성에 있어서, 마그네슘 이온은 하기한 메카니즘에 의해 촉매로서 작용하는 것으로 추정된다.In the present invention, magnesium may be added to the broiler feed composition in addition to blood meal, preferably in the form of MgO. MgO may use about 1 g per kg of feed. In carnosine synthesis, magnesium ions are assumed to act as catalysts by the mechanism described below.

Figure 112008065068928-pat00003
Figure 112008065068928-pat00003

본 발명에 따른 혈분을 첨가한 사료를 이용하여 육계를 사양한 결과, 육계의 주령이 증가할수록 혈분의 급여로 인한 근육 내 카노신 함량의 증가를 확인하였다.As a result of refraining broilers using the feed containing the blood powder according to the present invention, as the age of broilers increased, it was confirmed that the increase in the content of muscle carnosine due to the blood meal.

도 2에서 보이는 바와 같이, 대조구와 혈분 처리구 간의 카노신과 안세린 함량 증가는 반비례하는 것으로 확인되었는데, 대조구에서 혈분 10중량% 처리구로 갈수록 카노신 함량이 증가한 반면 안세린 함량은 반대로 감소하였다.As shown in FIG. 2, it was confirmed that the increase in the cannosine and anserine content between the control and the blood powder treatment was inversely proportional to the increase in the carnosine content while the anserine content was reversed.

본 발명에 따른 사료 조성물은 옥수수, 밀, 대두박, 옥수수 글루텐, 건조유청, 어분, 가금부산물, 지방, 무기질, 비타민 등을 함유할 수 있다. 다른 성분으로는 통상적으로 해바라기 박, 카놀라 박, 면실 박, 주정 박 등이 있으나, 이들에 제 한되지는 않는다.The feed composition according to the present invention may contain corn, wheat, soybean meal, corn gluten, dry whey, fish meal, poultry by-products, fats, minerals, vitamins and the like. Other ingredients typically include, but are not limited to, sunflower gourds, canola gourds, cotton gourd gourds, spirits gourds, and the like.

상기한 바와 같이 본 발명에 따라 혈분이 첨가된 사료를 육계에게 공급하므로써 일반 사료를 공급한 경우 보다 계육 조직 내에 카노신의 함량이 증가하게 된다.As described above, by supplying the feed with the added blood powder to the broiler according to the present invention, the content of carnosine in the broiler tissue is increased than when the feed is fed to the broiler.

카노신은 항산화작용, 혈관확장작용, 효소조절 및 신경전달 물질 역할 등의 작용이 있는 것으로 알려진 디펩티드로, 카노신을 별도로 섭취하지 않고 본 발명의 방법으로 사양되어 공급되는 계육조직 내의 카노신을 음식물 형태로 자연스럽게 섭취할 수 있다. 또한, 본 발명의 방법에 따라 사양된 육계는 그 조직 내에 카노신 함량이 일반 육계보다 높으므로 기능성 축산품으로서 시장에서 경쟁력을 가지게 되어 양계 농가의 소득 증대원으로 제공될 수 있다.Cannosine is a dipeptide known to have antioxidant, vasodilating, enzyme regulating and neurotransmitter roles, and cannosine in poultry tissue, which is specified and supplied by the method of the present invention without food intake, in the form of food. You can eat it naturally. In addition, broilers specified in accordance with the method of the present invention has a higher content of carnosine in the tissues than ordinary broilers, so that it is competitive in the market as a functional livestock product and can be provided as a source of income for poultry farmers.

이하, 본 발명을 실시예에 의하여 더욱 상세히 설명하면 다음과 같다.Hereinafter, the present invention will be described in more detail with reference to Examples.

1. 시험 설계1. Test design

시험은 1차와 2차 총 2회에 걸쳐 반복시험 하였으며 사양시험을 위해 갓 부화한 육계(Ross 종) 암, 수 각 96수를 공시하여 3처리 4반복, 반복당 암, 수 동수로 8수씩 케이지 (가로 2.0 m, 세로 2.4 m)에 완전임의 배치하였다.The test was repeated two times in the first and second rounds. For the specification test, the newly hatched broiler (Ross species) females and 96 females were disclosed. Fully placed in the cage (2.0 m wide, 2.4 m long).

시판되는 육계용 크럼블 사료 급여구를 대조구로 하였으며, 크럼블 사료에 각각 하기 표 1의 성분으로 표시되는 혈분(화인(주) 제품)을 사료 1 kg 당 50 g과 100 g을 첨가하여 혈분 5 중량%를 함유하는 사료와 혈분 10 중량%를 함유하는 사료를 각각 제조하였다. 각각에 카노신 합성 효소의 촉매로 MgO 1 g/kg을 혼합한 다음, 이 사료를 배합사료 처리구로 하여 시험을 설계하였다.A commercial broiler crumble feed diet was used as a control, and 50 ml and 100 g of blood meal (Fine Co., Ltd.) represented by the ingredients of Table 1 were added to the crumble feed, respectively. A feed containing wt% and a feed containing 10 wt% of blood meal were prepared, respectively. MgO as a catalyst of carnosine synthase on each The test was designed with 1 g / kg mixed and then using this feed as blended feed.

시험 시작 1주 후부터 1주 간격으로 반복 당 암, 수 각 1수씩을 도계하여 가슴 부위 근육 5 내지 20 g을 채취하였으며 총 5주간 이러한 과정을 진행하였다. 시험 기간 35일 동안 물과 사료를 자유 채식시켰고 24시간 점등하였다.One week after the start of the test, the number of cancers and the number of repetitions were repeated at 1 week intervals, and 5 to 20 g of chest muscles were collected. This process was performed for a total of 5 weeks. During the test period 35 days of water and feed were vegan and lit 24 hours.

혈분 중의 일반 성분과 아미노산 성분 분석표Table of general and amino acid components in blood 일반 성분 (%)General ingredient (%) 아미노산 성분 (%)Amino Acid Ingredient (%) 수분moisture 6.926.92 알라닌Alanine 7.337.33 조단백질Crude protein 91.8591.85 히스티딘Histidine 5.765.76 조지방Crude fat 0.090.09 리신Lee Sin 7.837.83 조섬유질Crude fiber 0.060.06 트레오닌Threonine 3.453.45 Crude AshCrude ash 1.061.06 아르기닌Arginine 3.783.78 메티오닌Methionine 0.220.22 시스틴Cystine 0.560.56 데이터 분석: Arirang BNS, Inc and Science Technique Analysis Center. Data Analysis: Arirang BNS, Inc and Science Technique Analysis Center.

2. 시료의 전처리2. Preparation of Sample

닭 가슴 시료를 채취한 후 3일 동안 동결건조 시켜 수분을 제거하였다. 완전히 수분이 제거된 후 (수분 70~75 %) 시료를 분쇄기로 곱게 갈아 30 mL 튜브 (Nal-gene) 에 1 g을 넣는다. 24 mL의 증류수를 넣어 혼합한 후 20분간 근육의 성분이 용해 되도록 한다.After taking the chicken breast sample was lyophilized for 3 days to remove moisture. After complete dehydration (70-75% moisture), grind the sample finely into a grinder and place 1 g in a 30 mL tube (Nal-gene). Add 24 mL of distilled water to mix and allow the muscle components to dissolve for 20 minutes.

그런 다음, 시료를 4℃, 10,000 rpm, radius 99 mm, 20 min 조건에서 원심분리 한 후 그 상층액을 조심스럽게 취하여 멤브레인 여과기로 여과하였다. 여과액 300 ㎕ 와 메탄올 900 ㎕를 혼합한 후 4℃ 냉장실에서 24 시간 동안 보관하여 단백질 응고과정을 거친 후 4℃, 12,000 rpm, radius 99 mm, 3 min 조건으로 원심분리 하여 그 상층액을 HPLC로 분석하였다.Then, the sample was centrifuged at 4 ° C., 10,000 rpm, radius 99 mm, and 20 min. The supernatant was carefully taken out and filtered through a membrane filter. After mixing 300 μl of the filtrate and 900 μl of methanol, the mixture was stored in a 4 ° C. refrigerator for 24 hours to undergo protein coagulation, followed by centrifugation at 4 ° C., 12,000 rpm, radius 99 mm, and 3 min. The supernatant was purified by HPLC. Analyzed.

3. 분석시약3. Analysis reagent

L- Carnosine 99%와 L- Anserine (Labelled as L- Anserine nitrate salt) (시그마사 제품, 스위스)으로 표준용액을 만들어 사용하였으며, HPLC 분석을 위한 시약으로서 아세토니트릴(ACN)과 메탄올 (J.T.　Baker사 제품 ; HPLC급)을 사용하였다. 테트라하이드로퓨란(THF), O-phthaldialdehyde (OPA), 2-머캡탄올, 아세트산은 시그마-알드리치(Sigma-Aldrich)사 제품을 사용하였다. 보릭산(Boric acid, (주)덕산제품)은 20 mM, pH 9.5 용액으로 만들어 사용하였다. L- Carnosine A standard solution was prepared using 99% and L- Anserine (Labelled as L- Anserine nitrate salt) (Sigma, Switzerland), and acetonitrile (ACN) and methanol (JT Baker; HPLC; Grade) was used. Tetrahydrofuran (THF), O-phthaldialdehyde (OPA), 2-mercaptanol, and acetic acid were used by Sigma-Aldrich. Boric acid (Boric acid, manufactured by Duksan Co., Ltd.) was used as a 20 mM, pH 9.5 solution.

4. HPLC 시스템4. HPLC system

카노신과 안세린의 분석조건은 Jens 등 (Jens Schonherr (2002). Analysis of products of animal orgin in feeds by determination of carnosine and related dipeptides by High performance Liquid Chromatography. Agricultuarl and food chemistry, 50, 1945-1950)을 참조하였다. Analysis of the conditions of Kano God and not the serine is such Jens (Jens Schonherr (2002). Analysis of products of animal orgin in feeds by determination of carnosine and related dipeptides by High performance Liquid Chromatography. Agricultuarl and food chemistry , 50, 1945-1950.

OPA 프리-컬럼(pre-column) 유도체 방법을 이용하였으며 HPLC (GILSON Model 305, France)와 형광검출기 (GILSON Model 121) 및 컬럼 (250 × 4.5 mm, P/N CS-2546-C185 ; Higgins)를 이용하였다. OPA와 시료는 100 ㎕ : 20 ㎕로 혼합하여 2분 동안 반응시켰다. OPA pre-column derivative method was used and HPLC (GILSON Model 305, France), fluorescence detector (GILSON Model 121) and column (250 × 4.5 mm, P / N CS-2546-C185; Higgins) were used. Was used. OPA and the sample were mixed with 100 μl: 20 μl and reacted for 2 minutes.

OPA 유도체는 OPA 2 mg을 메탄올 0.5 mL에 녹인 후 Borate buffer 20mM, 19.5 mL에 혼합한 후 2-머캡탄올 5㎕를 첨가하여 제조하였으며 빛을 차단한 병에 보관하였고 24시간 이내에 사용하였다. 이동상과 농도기울기 조건을 하기 표 2에 기재하였다.The OPA derivative was prepared by dissolving 2 mg of OPA in 0.5 mL of methanol, mixing 20 mM Borate buffer and 19.5 mL, and adding 5 μl of 2-mercaptanol. The OPA derivative was stored in a light-blocked bottle and used within 24 hours. Mobile phase and concentration gradient conditions are listed in Table 2 below.

이 동 상This statue 시 약 A Reagent A 아세트산 50 mM, pH 4.37 Acetic acid 50 mM, pH 4.37 시 약 B Reagent B ACN 70% : MeOH 25% : THF 5% ACN 70%: MeOH 25%: THF 5%
농도기울기
조건
Concentration
Condition 1) 0-25 min B 18% 1) 0-25 min B 18% 4) 31-32min B 18% 4) 31-32min B 18% 2) 25-26 min B 100 % 2) 25-26 min B 100% 5) 32-40 min B 18% 5) 32-40 min B 18% 3) 26-31 min B 100 % 3) 26-31 min B 100%

5. 통계분석5. Statistical Analysis

시험에서 얻어진 자료의 통계분석은 SAS (1995) GLM (General Linear Model) 방법을 이용하여 분석하였으며 처리구 평균 간의 유의성은 Duncan's multiple range test를 이용하여 신뢰도 P<0.05 범위에서 측정하였다. Statistical analysis of the data obtained was performed using SAS (1995) General Linear Model (GLM) method. The significance between treatment means was measured in the range of reliability P <0.05 using Duncan's multiple range test.

닭가슴살 중의 카노신 및 안세린 함량 분석 (건조중량, mg/g)Determination of Carnosine and Anserine Content in Chicken Breast (Dry Weight, mg / g) 카 노 신Cano Shin 1 회차1st round 2 회차2nd round 대조구Control 혈분 5%5% blood 혈분 10%10% blood SEMSEM 대조구Control 혈분 5%5% blood 혈분 10%10% blood SEMSEM 1 주1 week 9.819.81 9.229.22 11.7611.76 1.4831.483 8.878.87 8.918.91 7.297.29 1.2051.205 2 주2 weeks 9.84b 9.84 b 11.25ab 11.25 ab 13.90a 13.90 a 1.5211.521 15.39a 15.39 a 14.87a 14.87 a 12.34b 12.34 b 1.2031.203 3 주3 weeks 15.9415.94 19.1119.11 18.1418.14 2.6612.661 12.8212.82 13.9113.91 13.0813.08 0.9370.937 4 주4 weeks 14.78b 14.78 b 20.58a 20.58 a 22.33a 22.33 a 2.0742.074 10.24b 10.24 b 14.23a 14.23 a 13.06ab 13.06 ab 1.2471.247 5 주5 weeks 10.87b 10.87 b 13.71a 13.71 a 12.35ab 12.35 ab 1.2061.206 12.11b 12.11 b 15.64a 15.64 a 16.38a 16.38 a 1.3311.331
안 세 린
Anse Lin 1 주1 week 10.5310.53 9.109.10 6.536.53 1.2901.290 3.363.36 3.773.77 3.183.18 0.5410.541 2 주2 weeks 14.45a 14.45 a 11.09b 11.09 b 10.19b 10.19 b 1.7311.731 5.895.89 5.835.83 7.427.42 1.1661.166 3 주3 weeks 17.66a 17.66 a 14.58b 14.58 b 13.55b 13.55 b 1.3291.329 12.35a 12.35 a 10.26ab 10.26 ab 8.68b 8.68 b 1.3481.348 4 주4 weeks 18.47a 18.47 a 14.97b 14.97 b 14.81b 14.81 b 1.6481.648 15.36a 15.36 a 12.05ab 12.05 ab 11.30b 11.30 b 0.5410.541 5 주5 weeks 20.80a 20.80 a 17.16b 17.16 b 15.38b 15.38 b 1.7931.793 21.66a 21.66 a 20.27ab 20.27 ab 18.12b 18.12 b 1.8151.815 a-b Means in a row with no common superscript differ significantly (P<0.05) ab Means in a row with no common superscript differ significantly (P <0.05)

급여기간 동안(5주) 육계의 사료 섭취량 및 증체량Feed Intake and Weight Gain of Broilers During Feeding Period (5 weeks) 주차parking 처리군Treated group SEMSEM 대조구Control 혈분 5%5% blood 혈분 10%10% blood 사료
섭취량
g/마리feed
Intake
g / horse 1회차1st round 0-30-3 1030.801030.80 1008.341008.34 995.45995.45 32.98332.983 4-54-5 1693.951693.95 1640.951640.95 1599.251599.25 48.07248.072 0-50-5 2724.762724.76 2649.292649.29 2594.712594.71 73.92373.923 2회차2nd round 0-30-3 1216.34a 1216.34 a 1175.72a 1175.72 a 1116.04b 1116.04 b 19.60419.604 4-54-5 1882.401882.40 1885.901885.90 1792.41792.4 84.82184.821 0-50-5 3098.803098.80 3061.603061.60 2908.52908.5 87.14087.140 증체량,
g/마리Weight Gain,
g / horse 1회차1st round 0-30-3 791.46791.46 784.30784.30 752.19752.19 68.25268.252 4-54-5 939.00939.00 920.60920.60 1010.201010.20 104.147104.147 0-50-5 1730.431730.43 1704.931704.93 1762.361762.36 77.40077.400 2회차2nd round 0-30-3 863.50863.50 861.75861.75 823.50823.50 22.38722.387 4-54-5 1029.251029.25 1021.001021.00 995.25995.25 65.01665.016 0-50-5 1892.751892.75 1882.751882.75 1818.751818.75 58.73758.737 a-b Means in a row with no common superscript differ significantly (P<0.05) ab Means in a row with no common superscript differ significantly (P <0.05)

상기 표 3에서 알 수 있는 바와 같이, 1회차 및 2회차 시험을 비교하였을 때 주령이 증가할수록 혈분의 급여에 따라 계육 내의 카노신 함량이 증가하였다.As can be seen in Table 3, when comparing the first and second trials, the carnosine content in the poultry increased according to the blood meal as the age increased.

표 4는 5주 동안 대조구와 혈분 급여구의 사료 섭취량과 그에 따른 증체량을 나타낸 것으로, 수치상으로 보면 대조구가 혈분 처리구보다 사료 섭취량이 더 좋은 것으로 나타났다. 그러나 1차 시험의 경우 사료 섭취량이 대조구보다 낮은 혈분 10중량% 처리구에서 증체량이 높은 경우도 있어서 혈분의 첨가로 인한 사료의 섭취 감소가 육계의 생산성을 떨어뜨리는 것은 아닌 것을 확인했다.Table 4 shows the feed intake and the resulting gain in the control and blood meal groups for 5 weeks. Numerically, the control showed better feed intake than the blood meal. However, in the first trial, the feed intake was higher in the 10% by weight blood treatment than the control, and the increase in feed intake due to the addition of blood powder did not reduce the broiler productivity.

상술한 실시예, 시험예 및 도면은 발명의 내용을 상세히 설명하기 위한 목적일 뿐, 발명의 기술적 사상의 범위를 한정하고자 하는 목적이 아니며, 이상에서 설명한 본 발명은 본 발명이 속하는 기술분야에서 통상의 지식을 가진 자에 있어 본 발명의 기술적 사상을 벗어나지 않는 범위 내에서 여러 가지 치환, 변형 및 변경이 가능하므로 상기 실시예 및 첨부된 도면에 한정되는 것이 아님은 물론이며, 후술하는 청구범위 뿐만이 아니라 청구범위와 함께 균등범위를 포함하여 판단되어야 한다.The above embodiments, test examples and drawings are merely for the purpose of describing the contents of the invention in detail, and are not intended to limit the scope of the technical idea of the invention, and the present invention described above is usually in the technical field to which the present invention belongs. As those skilled in the art, various substitutions, modifications and changes are possible within the scope without departing from the spirit of the present invention is not limited to the embodiments and the accompanying drawings, as well as the claims described below It should be determined that the claims are included in their equality.

도 1은 본 발명에 따른 혈분을 첨가한 육계 사료를 급여한 5주간의 사양시험 기간 동안 1차 시험과 2차 시험으로부터 얻어진 육계 조직 내의 카노신과 안세린의 농도 변화를 나타낸 그래프이다. 매주 간의 그래프 상에서 ■와 ◆는 대조구(T1)와 혈분 5중량%(T2) 및 10중량%(T3)를 나타낸다.1 is a graph showing the change in the concentration of carnosine and anserine in broiler tissue obtained from the first test and the second test during the five-week feeding test diet fed broiler feed with blood powder according to the present invention. In the weekly graphs, ■ and ◆ represent the control (T1), 5% by weight (T2) and 10% by weight (T3) of blood.

Claims (3)

사료의 총 중량에 대하여 혈분(blood meal) 5 내지 10중량%를 첨가하여 얻어진 사료를 육계에게 공급하여서 육계를 사양하여 이루어지는 것을 특징으로 하는 계육 조직 내 카노신 함량의 증진방법.A method for increasing the content of cannosine in poultry tissue, characterized in that the broiler is characterized by feeding the feed obtained by adding 5 to 10% by weight of blood meal to the total weight of the feed. 제 1항에 있어서, 상기 혈분 이외에 추가로 MgO를 첨가하는 것을 특징으로 하는 계육 조직 내 카노신 함량의 증진방법.The method of claim 1, wherein MgO is added in addition to the blood meal. 제2항에 있어서, 상기 MgO를 사료 전체 중량에 대하여 1 : 0.001의 비율로 첨가하는 것을 특징으로 하는 계육 조직 내 카노신 함량의 증진방법.The method of claim 2, wherein the MgO is added at a ratio of 1: 0.001 based on the total weight of the feed.
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