KR20080113226A - 비천연 아미노산 페닐셀레노시스테인을 함유하는 단백질의 유전자적으로 프로그램된 발현 - Google Patents

비천연 아미노산 페닐셀레노시스테인을 함유하는 단백질의 유전자적으로 프로그램된 발현 Download PDF

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KR20080113226A
KR20080113226A KR1020087024575A KR20087024575A KR20080113226A KR 20080113226 A KR20080113226 A KR 20080113226A KR 1020087024575 A KR1020087024575 A KR 1020087024575A KR 20087024575 A KR20087024575 A KR 20087024575A KR 20080113226 A KR20080113226 A KR 20080113226A
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trna
protein
amino acid
phenylselenocysteine
amino acids
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English (en)
Korean (ko)
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지앙윤 왕
피터 지 슐츠
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더 스크립스 리서치 인스티튜트
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Publication of KR20080113226A publication Critical patent/KR20080113226A/ko
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/104—Aminoacyltransferases (2.3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
KR1020087024575A 2006-03-16 2007-03-13 비천연 아미노산 페닐셀레노시스테인을 함유하는 단백질의 유전자적으로 프로그램된 발현 Ceased KR20080113226A (ko)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US78327206P 2006-03-16 2006-03-16
US60/783,272 2006-03-16
US86145606P 2006-11-28 2006-11-28
US60/861,456 2006-11-28

Publications (1)

Publication Number Publication Date
KR20080113226A true KR20080113226A (ko) 2008-12-29

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KR1020087024575A Ceased KR20080113226A (ko) 2006-03-16 2007-03-13 비천연 아미노산 페닐셀레노시스테인을 함유하는 단백질의 유전자적으로 프로그램된 발현

Country Status (12)

Country Link
US (3) US7790847B2 (enExample)
EP (1) EP1994165B1 (enExample)
JP (1) JP5249194B2 (enExample)
KR (1) KR20080113226A (enExample)
CN (1) CN101405401B (enExample)
AU (1) AU2007227592B2 (enExample)
BR (1) BRPI0709603A2 (enExample)
CA (1) CA2638772A1 (enExample)
IL (1) IL193722A0 (enExample)
MX (1) MX2008011669A (enExample)
RU (1) RU2464315C2 (enExample)
WO (1) WO2007109035A2 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20150064209A (ko) * 2012-10-12 2015-06-10 서트로 바이오파마, 인크. 개선된 발현을 위해 박테리아 추출물 중 선택 단백질의 단백질분해 불활성화

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JP2005502322A (ja) * 2001-04-19 2005-01-27 ザ スクリップス リサーチ インスティテュート 非天然アミノ酸のインビボ組込み
EP1558747B1 (en) * 2002-10-16 2009-10-14 The Scripps Research Institute Glycoprotein synthesis
CN103215227B (zh) 2003-04-17 2015-09-16 斯克利普斯研究院 扩展真核生物遗传密码
ES2301099T3 (es) * 2003-07-07 2008-06-16 The Scripps Research Institute Composiciones de parejas ortogonales lisil-arnt y aminoacil-arnt sintetasa y usos de las mismas.
US8216804B2 (en) 2004-10-27 2012-07-10 The Scripps Research Institute Orthogonal translation components for the in vivo incorporation of unnatural amino acids
CN101400796B (zh) 2006-03-09 2013-03-27 斯克利普斯研究院 在真细菌宿主细胞内表达正交翻译组分的系统
RU2464315C2 (ru) 2006-03-16 2012-10-20 Зе Скрипс Ресеч Инститьют Генетически запрограммированная экспрессия белков, содержащих не встречающуюся в природе аминокислоту фенилселеноцистеин
US7807411B2 (en) * 2006-05-23 2010-10-05 The Scripps Research Institute Genetically encoded fluorescent coumarin amino acids
EP2052083B1 (en) * 2006-09-21 2014-01-08 The Scripps Research Institute Genetically programmed expression of selectively sulfated proteins in eubacteria
EP2069481A4 (en) 2006-10-18 2010-01-06 Scripps Research Inst GENETIC ASSEMBLY OF UNNATURAL AMINO ACIDS IN PROTEINS IN MAMMALIAN CELLS
JP5643100B2 (ja) 2007-10-25 2014-12-17 ザ スクリプス リサーチ インスティテュート レダクターゼへの３−アミノチロシンの遺伝的組込み
EP2217702A4 (en) 2007-11-02 2012-05-16 Scripps Research Inst DIRECTED EVOLUTION USING PROTEINS WITH NON-NATURAL AMINO ACIDS
WO2009059056A2 (en) * 2007-11-02 2009-05-07 The Scripps Research Institute A genetically encoded boronate amino acid
EP2889370A1 (en) 2007-12-11 2015-07-01 The Scripps Research Institute In vivo unnatural amino acid expression in the methylotrophic yeast Pichia pastoris
US8592199B2 (en) 2007-12-28 2013-11-26 David H. Ardell Genetic encryption
PL2238242T3 (pl) 2008-01-03 2015-06-30 Basf Enzymes Llc Transferazy i oksydoreduktazy, kwasy nukleinowe kodujące je oraz sposoby ich wytwarzania i zastosowania
JP2011514322A (ja) * 2008-02-08 2011-05-06 ザ スクリプス リサーチ インスティチュート 遺伝的にコードされる非天然アミノ酸による免疫寛容解除
WO2010068278A2 (en) 2008-12-10 2010-06-17 The Scripps Research Institute Production of carrier-peptide conjugates using chemically reactive unnatural amino acids
US20090197339A1 (en) * 2008-12-10 2009-08-06 The Scripps Research Institute In vivo unnatural amino acid expression in the methylotrophic yeast pichia pastoris
US8658394B2 (en) * 2011-02-02 2014-02-25 Waterstone Pharmaceuticals Methods, cells and systems for incorporating non-canonical amino acids into proteins
CN103215235B (zh) * 2012-01-18 2014-11-05 中国科学院生物物理研究所 3-咪唑基酪氨酸翻译系统及其应用
CN103571804B (zh) * 2012-08-10 2015-08-12 中国科学院生物物理研究所 3-吡唑基酪氨酸翻译系统及其应用
CN103820410B (zh) * 2012-11-16 2016-03-30 中国科学院生物物理研究所 3-甲硫酪氨酸翻译系统及其应用
CN104004723B (zh) * 2013-02-22 2016-08-17 中国科学院生物物理研究所 3,5-二氟代酪氨酸翻译系统及其应用
CN106047846B (zh) * 2013-03-22 2020-02-04 中国科学院生物物理研究所 8-羟基喹啉丙氨酸翻译系统及其应用
ES2927607T3 (es) 2013-09-13 2022-11-08 Scripps Research Inst Agentes terapéuticos modificados y composiciones de los mismos
EP3082797A4 (en) 2013-12-18 2017-12-13 The California Institute for Biomedical Research Modified therapeutic agents, stapled peptide lipid conjugates, and compositions thereof
PL3458074T3 (pl) * 2016-05-16 2024-11-12 Board Of Regents Of The University Of Texas System KOMPOZYCJA DO DOSTARCZANIA tRNA W POSTACI NANOCZĄSTEK I SPOSOBY ICH STOSOWANIA
EP3565904A1 (en) 2016-12-29 2019-11-13 Illumina, Inc. Analysis system for orthogonal access to and tagging of biomolecules in cellular compartments
CN114644581B (zh) * 2021-01-04 2024-07-16 北京惠大生物科技有限公司 含芳基硫酚或芳基硒酚经修饰的氨基酸、重组蛋白及其生物合成方法及应用
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CN118389493B (zh) * 2024-05-10 2025-04-11 北京大学 一种定点修饰的工程化tRNA及其应用

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CN101400796B (zh) 2006-03-09 2013-03-27 斯克利普斯研究院 在真细菌宿主细胞内表达正交翻译组分的系统
RU2464315C2 (ru) 2006-03-16 2012-10-20 Зе Скрипс Ресеч Инститьют Генетически запрограммированная экспрессия белков, содержащих не встречающуюся в природе аминокислоту фенилселеноцистеин

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KR20150064209A (ko) * 2012-10-12 2015-06-10 서트로 바이오파마, 인크. 개선된 발현을 위해 박테리아 추출물 중 선택 단백질의 단백질분해 불활성화
US10450353B2 (en) 2012-10-12 2019-10-22 Sutro Biopharma, Inc. Proteolytic inactivation of select proteins in bacterial extracts for improved expression
US11261219B2 (en) 2012-10-12 2022-03-01 Sutro Biopharma, Inc. Proteolytic inactivation of select proteins in bacterial extracts for improved expression

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Publication number Publication date
US20070238152A1 (en) 2007-10-11
US20120282668A1 (en) 2012-11-08
CN101405401A (zh) 2009-04-08
IL193722A0 (en) 2011-08-01
AU2007227592A1 (en) 2007-09-27
US20090209000A1 (en) 2009-08-20
JP2009529877A (ja) 2009-08-27
BRPI0709603A2 (pt) 2011-07-19
RU2008135114A (ru) 2010-04-27
EP1994165B1 (en) 2014-11-05
AU2007227592B2 (en) 2011-11-24
US8217145B2 (en) 2012-07-10
CN101405401B (zh) 2013-03-27
MX2008011669A (es) 2008-09-22
WO2007109035A3 (en) 2008-10-16
JP5249194B2 (ja) 2013-07-31
EP1994165A4 (en) 2012-11-07
CA2638772A1 (en) 2007-09-27
US7790847B2 (en) 2010-09-07
RU2464315C2 (ru) 2012-10-20
US8394605B2 (en) 2013-03-12
WO2007109035A2 (en) 2007-09-27
EP1994165A2 (en) 2008-11-26

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