RU2464315C2 - Генетически запрограммированная экспрессия белков, содержащих не встречающуюся в природе аминокислоту фенилселеноцистеин - Google Patents

Генетически запрограммированная экспрессия белков, содержащих не встречающуюся в природе аминокислоту фенилселеноцистеин Download PDF

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RU2464315C2
RU2464315C2 RU2008135114/10A RU2008135114A RU2464315C2 RU 2464315 C2 RU2464315 C2 RU 2464315C2 RU 2008135114/10 A RU2008135114/10 A RU 2008135114/10A RU 2008135114 A RU2008135114 A RU 2008135114A RU 2464315 C2 RU2464315 C2 RU 2464315C2
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trna
protein
amino acid
phenylselenocysteine
sequence
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RU2008135114A (ru
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Джиангюн ВАНГ (US)
Джиангюн ВАНГ
Питер Г. ШУЛЬЦ (US)
Питер Г. ШУЛЬЦ
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Зе Скрипс Ресеч Инститьют
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/104—Aminoacyltransferases (2.3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C—CHEMISTRY; METALLURGY
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    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products

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  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
RU2008135114/10A 2006-03-16 2007-03-13 Генетически запрограммированная экспрессия белков, содержащих не встречающуюся в природе аминокислоту фенилселеноцистеин RU2464315C2 (ru)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US78327206P 2006-03-16 2006-03-16
US60/783,272 2006-03-16
US86145606P 2006-11-28 2006-11-28
US60/861,456 2006-11-28

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RU2008135114A RU2008135114A (ru) 2010-04-27
RU2464315C2 true RU2464315C2 (ru) 2012-10-20

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US (3) US8217145B2 (enExample)
EP (1) EP1994165B1 (enExample)
JP (1) JP5249194B2 (enExample)
KR (1) KR20080113226A (enExample)
CN (1) CN101405401B (enExample)
AU (1) AU2007227592B2 (enExample)
BR (1) BRPI0709603A2 (enExample)
CA (1) CA2638772A1 (enExample)
IL (1) IL193722A0 (enExample)
MX (1) MX2008011669A (enExample)
RU (1) RU2464315C2 (enExample)
WO (1) WO2007109035A2 (enExample)

Cited By (2)

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RU2771892C2 (ru) * 2016-12-29 2022-05-13 Иллумина, Инк. Система анализа для ортогонального доступа к биомолекулам и их мечения в клеточных компартментах
US12416002B2 (en) 2016-12-29 2025-09-16 Illumina, Inc. Analysis system for orthogonal access to and tagging of biomolecules in cellular compartments

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SG160203A1 (en) * 2002-10-16 2010-04-29 Scripps Research Inst Glycoprotein synthesis
CN101223272B (zh) 2003-04-17 2013-04-03 斯克利普斯研究院 扩展真核生物遗传密码
EP1649004A4 (en) 2003-07-07 2008-04-09 Scripps Research Inst COMPOSITIONS WITH PAIRS OF ORTHOGONAL LYSYL-TRNA AND AMINOACYL-TRNA-SYNTHETASE AND USES THEREOF
EP1807515A4 (en) * 2004-10-27 2008-06-18 Scripps Research Inst Orthogonal translation components for the in vivo incorporation of unnatural amino acids
AU2007223830A1 (en) * 2006-03-09 2007-09-13 The Scripps Research Institute System for the expression of orthogonal translation components in eubacterial host cells
EP1994165B1 (en) 2006-03-16 2014-11-05 The Scripps Research Institute Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine
AU2007267950B2 (en) * 2006-05-23 2012-08-16 The Scripps Research Institute Genetically encoded fluorescent coumarin amino acids
US8114627B2 (en) 2006-09-21 2012-02-14 The Scripps Research Institute Genetically programmed expression of selectively sulfated proteins in eubacteria
RU2009112104A (ru) 2006-10-18 2010-11-27 Зе Скрипс Ресеч Инститьют (Us) Генетическое встраивание не встречающихся в природе аминокислот в белки клеток млекопитающих
WO2009055616A2 (en) 2007-10-25 2009-04-30 The Scripps Research Institute Genetic incorporation of 3-aminotyrosine into reductases
US9249408B2 (en) * 2007-11-02 2016-02-02 The Scripps Research Institute Directed evolution using proteins comprising unnatural amino acids
WO2009059056A2 (en) * 2007-11-02 2009-05-07 The Scripps Research Institute A genetically encoded boronate amino acid
AU2008335778B2 (en) * 2007-12-11 2014-04-10 The Scripps Research Institute In vivo unnatural amino acid expression in the methylotrophic yeast Pichia pastoris
WO2009086497A2 (en) * 2007-12-28 2009-07-09 Ardell David H Genetic encryption
CA2710683A1 (en) 2008-01-03 2009-07-16 Verenium Corporation Transferases and oxidoreductases, nucleic acids encoding them and methods for making and using them
AU2009210677A1 (en) * 2008-02-08 2009-08-13 The Scripps Research Institute Breaking immunological tolerance with a genetically encoded unnatural amino acid
US20090197339A1 (en) * 2008-12-10 2009-08-06 The Scripps Research Institute In vivo unnatural amino acid expression in the methylotrophic yeast pichia pastoris
US8637306B2 (en) 2008-12-10 2014-01-28 The Scripps Research Institute Production of carrier-peptide conjugates using chemically reactive unnatural amino acids
US8658394B2 (en) * 2011-02-02 2014-02-25 Waterstone Pharmaceuticals Methods, cells and systems for incorporating non-canonical amino acids into proteins
CN103215235B (zh) * 2012-01-18 2014-11-05 中国科学院生物物理研究所 3-咪唑基酪氨酸翻译系统及其应用
CN103571804B (zh) * 2012-08-10 2015-08-12 中国科学院生物物理研究所 3-吡唑基酪氨酸翻译系统及其应用
KR102018863B1 (ko) 2012-10-12 2019-09-05 서트로 바이오파마, 인크. 개선된 발현을 위해 박테리아 추출물 중 선택 단백질의 단백질분해 불활성화
CN103820410B (zh) * 2012-11-16 2016-03-30 中国科学院生物物理研究所 3-甲硫酪氨酸翻译系统及其应用
CN104004723B (zh) * 2013-02-22 2016-08-17 中国科学院生物物理研究所 3,5-二氟代酪氨酸翻译系统及其应用
CN104059891B (zh) * 2013-03-22 2016-08-17 中国科学院生物物理研究所 8-羟基喹啉丙氨酸翻译系统及其应用
JP6822839B2 (ja) * 2013-09-13 2021-01-27 ザ・スクリップス・リサーチ・インスティテュート 修飾された治療剤、及びその組成物
US10039809B2 (en) 2013-12-18 2018-08-07 The California Institute For Biomedical Research Modified therapeutic agents, stapled peptide lipid conjugates, and compositions thereof
DK3458074T3 (da) * 2016-05-16 2024-07-29 Univ Texas COMPOSITIONS FOR THE DELIVERY OF tRNA AS NANOPARTICLES AND METHODS OF USE THEREWITH
CN114644581B (zh) * 2021-01-04 2024-07-16 北京惠大生物科技有限公司 含芳基硫酚或芳基硒酚经修饰的氨基酸、重组蛋白及其生物合成方法及应用
KR20240032010A (ko) 2021-06-09 2024-03-08 더 스크립스 리서치 인스티튜트 장기 지속형 이중 gip/glp-1 펩타이드 접합체 및 사용 방법
CN118389493B (zh) * 2024-05-10 2025-04-11 北京大学 一种定点修饰的工程化tRNA及其应用

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Cited By (2)

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Publication number Priority date Publication date Assignee Title
RU2771892C2 (ru) * 2016-12-29 2022-05-13 Иллумина, Инк. Система анализа для ортогонального доступа к биомолекулам и их мечения в клеточных компартментах
US12416002B2 (en) 2016-12-29 2025-09-16 Illumina, Inc. Analysis system for orthogonal access to and tagging of biomolecules in cellular compartments

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CN101405401A (zh) 2009-04-08
EP1994165A2 (en) 2008-11-26
WO2007109035A3 (en) 2008-10-16
MX2008011669A (es) 2008-09-22
EP1994165B1 (en) 2014-11-05
RU2008135114A (ru) 2010-04-27
US7790847B2 (en) 2010-09-07
JP5249194B2 (ja) 2013-07-31
WO2007109035A2 (en) 2007-09-27
US8394605B2 (en) 2013-03-12
CN101405401B (zh) 2013-03-27
US20070238152A1 (en) 2007-10-11
BRPI0709603A2 (pt) 2011-07-19
US20120282668A1 (en) 2012-11-08
CA2638772A1 (en) 2007-09-27
JP2009529877A (ja) 2009-08-27
IL193722A0 (en) 2011-08-01
US20090209000A1 (en) 2009-08-20
KR20080113226A (ko) 2008-12-29
AU2007227592A1 (en) 2007-09-27
US8217145B2 (en) 2012-07-10
AU2007227592B2 (en) 2011-11-24
EP1994165A4 (en) 2012-11-07

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