CN101405401B - 含非天然氨基酸苯基硒代半胱氨酸的蛋白质的遗传编程表达 - Google Patents

含非天然氨基酸苯基硒代半胱氨酸的蛋白质的遗传编程表达 Download PDF

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CN101405401B
CN101405401B CN2007800092529A CN200780009252A CN101405401B CN 101405401 B CN101405401 B CN 101405401B CN 2007800092529 A CN2007800092529 A CN 2007800092529A CN 200780009252 A CN200780009252 A CN 200780009252A CN 101405401 B CN101405401 B CN 101405401B
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trna
amino acid
alpha
natural amino
protein
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CN101405401A (zh
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王江云
P·G·舒尔茨
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Scripps Research Institute
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/104—Aminoacyltransferases (2.3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
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  • Organic Chemistry (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
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  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
CN2007800092529A 2006-03-16 2007-03-13 含非天然氨基酸苯基硒代半胱氨酸的蛋白质的遗传编程表达 Expired - Fee Related CN101405401B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US78327206P 2006-03-16 2006-03-16
US60/783,272 2006-03-16
US86145606P 2006-11-28 2006-11-28
US60/861,456 2006-11-28
PCT/US2007/006382 WO2007109035A2 (en) 2006-03-16 2007-03-13 Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine

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CN101405401A CN101405401A (zh) 2009-04-08
CN101405401B true CN101405401B (zh) 2013-03-27

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US (3) US8217145B2 (enExample)
EP (1) EP1994165B1 (enExample)
JP (1) JP5249194B2 (enExample)
KR (1) KR20080113226A (enExample)
CN (1) CN101405401B (enExample)
AU (1) AU2007227592B2 (enExample)
BR (1) BRPI0709603A2 (enExample)
CA (1) CA2638772A1 (enExample)
IL (1) IL193722A0 (enExample)
MX (1) MX2008011669A (enExample)
RU (1) RU2464315C2 (enExample)
WO (1) WO2007109035A2 (enExample)

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CA2500653A1 (en) * 2002-10-16 2004-04-29 The Scripps Research Institute Glycoprotein synthesis
MXPA05011127A (es) 2003-04-17 2006-03-10 Scripps Research Inst Expansion del codigo genetico eucariontico.
AU2004267359B2 (en) * 2003-07-07 2010-09-09 The Scripps Research Institute Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof
WO2006110182A2 (en) * 2004-10-27 2006-10-19 The Scripps Research Institute Orthogonal translation components for the in vivo incorporation of unnatural amino acids
JP5589186B2 (ja) 2006-03-09 2014-09-17 ザ スクリプス リサーチ インスティテュート 真正細菌宿主細胞における直交翻訳成分の発現システム
US8217145B2 (en) 2006-03-16 2012-07-10 The Scrips Research Institute Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine
RU2473690C2 (ru) * 2006-05-23 2013-01-27 Зе Скрипс Ресеч Инститьют Генетически кодируемые флюоресцентные кумариновые аминокислоты
PL2052083T3 (pl) * 2006-09-21 2014-06-30 Scripps Research Inst Genetycznie programowana ekspresja selektywnie siarczanowych białek u eubakterii
WO2008073184A2 (en) 2006-10-18 2008-06-19 The Scripps Research Institute Genetic incorporation of unnatural amino acids into proteins in mammalian cells
CN102203257B (zh) 2007-10-25 2014-01-08 斯克利普斯研究院 3-氨基酪氨酸遗传掺入还原酶
US9150849B2 (en) 2007-11-02 2015-10-06 The Scripps Research Institute Directed evolution using proteins comprising unnatural amino acids
EP2220218A4 (en) * 2007-11-02 2010-12-08 Scripps Research Inst GENETICALLY ENCODED BORONATE AMINO ACID
JP2011527886A (ja) * 2007-12-11 2011-11-10 ザ スクリプス リサーチ インスティチュート メタノール資化性酵母ピキア・パストリスにおけるインビボ非天然アミノ酸発現
WO2009086497A2 (en) * 2007-12-28 2009-07-09 Ardell David H Genetic encryption
DK2238242T5 (en) 2008-01-03 2015-08-03 Basf Enzymes Llc -Transferases and oxidoreductases, nucleic acids encoding FOR THEM, AND PROCESSES FOR THE STILL LING AND USE THEREOF
CN101970005A (zh) * 2008-02-08 2011-02-09 斯克利普斯研究院 用遗传编码的非天然氨基酸打破免疫耐受
US8609383B2 (en) 2008-12-10 2013-12-17 The Scripps Research Institute Production of carrier-peptide conjugates using chemically reactive unnatural amino acids
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US8658394B2 (en) * 2011-02-02 2014-02-25 Waterstone Pharmaceuticals Methods, cells and systems for incorporating non-canonical amino acids into proteins
CN103215235B (zh) * 2012-01-18 2014-11-05 中国科学院生物物理研究所 3-咪唑基酪氨酸翻译系统及其应用
CN103571804B (zh) * 2012-08-10 2015-08-12 中国科学院生物物理研究所 3-吡唑基酪氨酸翻译系统及其应用
AU2013329464C1 (en) * 2012-10-12 2018-02-15 Sutro Biopharma, Inc. Proteolytic inactivation of select proteins in bacterial extracts for improved expression
CN103820410B (zh) * 2012-11-16 2016-03-30 中国科学院生物物理研究所 3-甲硫酪氨酸翻译系统及其应用
CN104004723B (zh) * 2013-02-22 2016-08-17 中国科学院生物物理研究所 3,5-二氟代酪氨酸翻译系统及其应用
CN106047846B (zh) * 2013-03-22 2020-02-04 中国科学院生物物理研究所 8-羟基喹啉丙氨酸翻译系统及其应用
JP6822839B2 (ja) 2013-09-13 2021-01-27 ザ・スクリップス・リサーチ・インスティテュート 修飾された治療剤、及びその組成物
CN106456589B (zh) 2013-12-18 2020-07-07 斯克利普斯研究所 修饰的治疗剂、订合的肽脂质缀合物及其组合物
PL3458074T3 (pl) * 2016-05-16 2024-11-12 Board Of Regents Of The University Of Texas System KOMPOZYCJA DO DOSTARCZANIA tRNA W POSTACI NANOCZĄSTEK I SPOSOBY ICH STOSOWANIA
CA3048863A1 (en) 2016-12-29 2018-07-05 Illumina, Inc. Analysis system for orthogonal access to and tagging of biomolecules in cellular compartments
CN114644581B (zh) * 2021-01-04 2024-07-16 北京惠大生物科技有限公司 含芳基硫酚或芳基硒酚经修饰的氨基酸、重组蛋白及其生物合成方法及应用
JP2024522196A (ja) 2021-06-09 2024-06-11 ザ スクリプス リサーチ インスティテュート 長時間作用型デュアルｇｉｐ／ｇｌｐ－１ペプチドコンジュゲートおよび使用方法
CN118389493B (zh) * 2024-05-10 2025-04-11 北京大学 一种定点修饰的工程化tRNA及其应用

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JP5249194B2 (ja) 2013-07-31
RU2008135114A (ru) 2010-04-27
US8394605B2 (en) 2013-03-12
MX2008011669A (es) 2008-09-22
IL193722A0 (en) 2011-08-01
US20090209000A1 (en) 2009-08-20
US20120282668A1 (en) 2012-11-08
US8217145B2 (en) 2012-07-10
KR20080113226A (ko) 2008-12-29
AU2007227592A1 (en) 2007-09-27
US7790847B2 (en) 2010-09-07
CA2638772A1 (en) 2007-09-27
RU2464315C2 (ru) 2012-10-20
BRPI0709603A2 (pt) 2011-07-19
EP1994165B1 (en) 2014-11-05
WO2007109035A2 (en) 2007-09-27
US20070238152A1 (en) 2007-10-11
JP2009529877A (ja) 2009-08-27
CN101405401A (zh) 2009-04-08
EP1994165A2 (en) 2008-11-26
AU2007227592B2 (en) 2011-11-24
WO2007109035A3 (en) 2008-10-16
EP1994165A4 (en) 2012-11-07

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