KR20080106770A - Composition for preventing and treating arthritis comprising the extracts of natural herb medicine - Google Patents

Abstract

A composition for preventing and treating arthritis, and a method for preparing the composition are provided to improve the treatment effect of arthritis without side effect. A composition for preventing and treating arthritis contains an extract of Eleutherococcus senticosus and an extract of Gardeniae Fructus as an active ingredient. Preferably the extract of Eleutherococcus senticosus and the extract of Gardeniae Fructus are a hot water extract of Eleutherococcus senticosus and Gardeniae Fructus with water, an alcohol or a mixture of water and an alcohol.

Description

Composition for preventing and treating arthritis comprising the extracts of natural herb medicine}

Figure 1 shows the effect of the natural herbal extracts of the present invention on the inhibition of inflammatory substances, nitric oxide (NO).

Figure 2 shows the effect of natural herbal extracts of the present invention on the inhibition of inflammatory substances, tumor necrosis factor-alpha (TNF-α).

The present invention relates to a composition for preventing and treating arthritis, which contains a natural herbal extract as an active ingredient. More specifically, the present invention relates to a composition for the prevention and treatment of arthritis due to aging, such as degenerative arthritis, which contains thorny scab and gardenia extract as an active ingredient, further comprising glucosamine, chondroitin, collagen, vitamins, and the like.

Arthritis is a general name that causes inflammatory changes in the musculoskeletal and connective tissues of the body, resulting in systemic symptoms in the musculoskeletal system. The disease is characterized by chronic inflammation that affects the joints, bones, cartilage tissue or spinal cord, often causing permanent tissue damage, malformations, degeneration and disorders (Hofbause, LC, Heufelder, AE: Arthritis and Rheumatism 44: 253). -259, 2001). Arthritis is classified as degenerative arthritis (osteoarthritis), rheumatoid arthritis, extra-articular rheumatoid or collagen disease. The most common degenerative arthritis among arthritis diseases is a disease in which local degenerative changes occur as the articular cartilage wears off. Although the cause is uncertain, it is known that the disease is deeply related to aging or excessive weight, in which the degenerative change of articular cartilage is the first. As the disease progresses, hardening of the subchondral bone, hyperplasia of the bone around the joint, deformation of the joint, and the like, the surface of the cartilage becomes rough and the inflammatory response is repeated in the joint cavity wrapped in the joint membrane. As a result, repetitive pain, joint stiffness, gradual movement disorder of the joint, and the like are caused. This disease is the most common inflammatory disease of the joints, when the age of 55 or more is about 80%, 75-year-old almost all populations are known to have this disease. Only about a quarter of these symptoms are clinical. It is more common in women than men, and it is severe.

Currently, clinically used degenerative arthritis can be treated with medications (painkillers, steroids, nonsteroidal anti-inflammatory drugs), cartilage protectors (hyaluronic acid, glucosamine, chondroitin, etc.) or surgical treatment (arthroscopy, proximal tibia). Osteotomy, joint replacement, total knee arthroplasty, etc.). However, drug therapy has only the effect of non-specifically alleviating the pain or inflammatory response itself, cartilage protector only serves to protect the joints by nourishing chondrocytes or alleviating shock. In addition, steroidal agents have side effects that can lead to osteoporosis, high blood pressure, diabetes, etc., due to loss of calcium when taken for a long time. Therefore, most of the drug treatment is used only for the purpose of reducing pain, and permanent arthroplasty is the main cause, but there are no drugs or surgical methods that give a fundamental therapeutic effect until now.

Although much research and efforts have been made to treat degenerative arthritis in the past decades, the pathological cause and the underlying treatment are not yet developed. Therefore, there is an urgent need for the development of new substances effective for the treatment and prevention of arthritis having a new function and skeletal structure that minimize the toxicity and side effects that are the limitations of current therapeutic agents of arthritis.

Therefore, the inventors of the present invention, while conducting various physiological activities of thorny scabies and gardenia, the herbal extracts such as thorny scabies and gardenia were not toxic, and the effect of inhibiting pain, which is the main clinical symptom of inflammatory substance and arthritis, was observed. As a result of the clinical trials by double-blind method, it was confirmed that the glucosamine salt, chondroitin, and collagen and the like were effective in improving arthritis significantly compared to the placebo administration group. Was completed.

Accordingly, an object of the present invention is to provide a composition for the prevention and treatment of arthritis using natural herbal extracts, which are effective in treating arthritis, but have no side effects.

The object of the present invention is to prepare an extract concentrate or powder of thorny scabies and gardenia to prepare powders, tablets, capsules and liquids for arthritis improvement, and to inhibit NO glass inhibition and TNF-α glass of extracts of thorny scabies and gardenia It was achieved by investigating the efficacy of the composition through the analgesic inhibitory effect, acute toxicity test, and simple clinical test using the liquid composition in the formulation.

According to one aspect of the invention, the present invention provides a composition for the prevention and treatment of arthritis, containing the extract of thorn ogapi and the extract of gardenia as an active ingredient.

In the present invention, the extract of the thorny opiaceae and the extract of gardenia may be extracted with a hydrophilic organic solvent such as water or alcohol, or a mixed solvent thereof, but preferably, the water, alcohol or a mixture of water and alcohol It is characterized in that the hot water extract in solution.

In the present invention, the extracts and extracts of Gardenia vulgaris shall include any one of all extracts, fractions and purified products obtained in each step of extraction, fractionation and purification, dilutions or concentrates thereof, or dried products thereof.

In the present invention, preferably, the extract of Prickly Oyster and Gardenia jasminoides is characterized in that the extract is concentrated and filtered or concentrated to extract the hot water or lyophilized powder form.

In the present invention, preferably the composition for preventing and treating arthritis is 0.5 to 30.0% by weight of thorny thorn extract, 0.1 to 20% by weight of gardenia extract, 5 to 90% by weight of glucosamine salt, 0.1 to 5.0% by weight of chondroitin , 0.1-10.0 wt% collagen, 0.01-1.0 wt% vitamin C, 0.05-0.1 wt% vitamin A, 0.01-0.1 wt% vitamin E, characterized in that consisting of.

In the present invention, the arthritis includes any arthritis symptomatic arthritis symptoms in the NO glass or TNF-α glass, preferably provides a composition for the prevention and treatment of arthritis, characterized in that rheumatoid arthritis or degenerative arthritis.

In the present invention, the composition may be used as a pharmaceutical composition for preventing and treating arthritis, as well as health foods or additives thereof, such as health drinks or beverage additives.

In the present invention, the composition may be in powder, tablet, capsule or liquid form depending on the dosage form.

The composition for preventing and treating arthritis containing the thorn stem extract and gardenia extract of the present invention as an active ingredient is characterized in that it is prepared through the following process:

Strictly screen and wash the gardenia or gardenia, dry it in the shade, chop it or grind it, and then add 1 to 30 times water, alcohol, or a mixed solution of water and alcohol per 1 kg in weight to 60 ~ 120 ℃. 1 to 10 times repeatedly for 1 hour to 10 days in hot water extraction to obtain a crude extract,

The crude extract is obtained by filtration, darkening or vacuum concentration to obtain an extract concentrate of 20 to 60 briquettes, or freeze-drying the crude extract to obtain a powder form.

Extraction conditions of the crude extract is 1 to 10 times, preferably 2 to about 1 hour to 2 days, preferably about 2 to 12 hours at 60 ~ 120 ℃, preferably 80 ~ 110 ℃ with purified water The crude extract can be obtained by extracting methods such as hot water extraction, ultrasonic extraction, reflux cooling extraction, etc., more preferably five to five times, and more preferably at a temperature of 80 to 110 ° C. for 1 to 5 hours.

Prickly Ogapi used in the present invention is a dry root and root and root and bark of Acanthopanax senticosus (RUPR. Et MAXIM) HARMS, which is a deciduous shrub of Araliaceae , classified as a medicinal herb that has no toxicity and side effects in Chinese medicine. Use peel as a medicine. The leaves have chisanoside and have pharmacological functions. In the roots, there are syrupin and coumarin glycosides as well as organo glycosides. Ogapi contains acanthoside B, D, which is an ogapi glycoside, and a water-soluble polysaccharide that enhances immunity. The pharmacological action of psoriasis that has been discovered to date has been reported to be sedative, antistress, immune enhancing, smooth muscle relaxation, anti-inflammatory. (國家 中 醫藥 管理局: 中華 本草 理, 海海, 上海 科學 技術 出版社, 1998 ), Dry fertilization (建 脾 益氣), Boxin Anxin (補腎 安神), Boxin coercion, Ikgianshin (활 安神), active blood circulation (Activ 血 通 絡) is known for its efficacy Body deprivation due to vanity, appetite deprivation and Yosul-san, body deprivation due to non-Xinyanghe It has been widely used in the treatment of blood loss, loss of appetite, loss of appetite, schizophrenia, pediatric musculoskeletal disorders, hydrocephalus, rheumatoid arthritis, etc. (國家 中 醫藥 管理局: 中華 本草, 上海, 上海 科學 技術 出版社, 1998).

Gardenia ( Fructus ) is the fruit of gardenia jasmoides Ellis or other cohorts belonging to the Rubiaceae family, and has been used as a food coloring agent since ancient times. In one shot, anti-inflammatory, diuretic, hemostatic. Although antipyretic, soothing, antibacterial, bile secretion, hepatitis is used for jaundice, hemorrhage, etc., but there is not much research on pain control when applied to anti-inflammatory, arthritis, fracture of gardenia.

Glucosamine, a kind of natural amino sugars, is used as a salt in the form of a salt for treating osteoarthritis in Europe. Glucosamine is present as a structural unit in sugar chains of various complex sugars in vivo. Representative of these are proteoglycans containing mucopolysaccharides of chondroitin sulfate and hyaluronic acid, and are widely distributed in skin tissue, cartilage and joint fluid. Collagen is a major protein constituting the connective tissue. It is a major component of skin, tendons, cartilage, muscles, bones, etc. It supports shape, absorbs external shocks, and maintains elasticity of tissues. It also acts to keep the proteoclican in place.

Vitamins also increase resistance to disease and prevent aging.

The composition for preventing and treating arthritis of the present invention gradually progresses after the 40's, and degenerative changes appear in the cartilage and the surrounding bones that make up the joint in the 50's. It is effective to improve diseases caused by physical aging, such as degenerative arthritis.

Such pharmaceutical compositions of the present invention may be administered orally or parenterally, and may be formulated into a suitable formulation using known techniques. In the preparation of the formulation, it is preferred to mix the active ingredient with the carrier, to dilute it with the carrier, or to enclose it in a carrier in the form of a capsule, assay or other container. Thus, the formulations may be in the form of tablets, pills, powders, assays, suspensions, emulsions, solutions, syrups, aerosols, soft or hard gelatin capsules, injectable solutions or suspensions and the like. Examples of suitable carriers, excipients and diluents are conventional sugars such as lactose, dextrose, sucrose and sugar alcohols such as xylitol, sorbitol, erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (tautin, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The formulation may further comprise fillers, anti-coagulants, lubricants, wetting agents, fragrances, emulsifiers, preservatives and the like.

In addition, it is appropriate to administer the extract of the thorn stem and gardenia which are the active ingredients of the composition of the present invention so that the daily intake of adult 1 ~ 3000mg. In addition, the dosage can be appropriately increased or decreased depending on age, symptoms, and the like.

An embodiment of the present invention comprises the steps of preparing the extract concentrate or powder of thorn stem and gardenia: preparation of the preparation for arthritis improvement: experiment of the efficacy of the preparation.

Hereinafter, the present invention will be described in more detail with reference to Examples. Since these examples are only for illustrating the present invention, the scope of the present invention is not to be construed as being limited by these examples.

Example 1: Preparation of natural herbal extracts

1. Preparation of thorny ogapi extract

Ogapi was washed thoroughly with water and dried in the shade. 500 g of dried shredded shredder was placed in a 10L extraction container, and 5L of purified water was added thereto, followed by hot water extraction at 100 ° C for 3 hours. After repeating the process three times, the resulting solution was cooled to 60 ℃ and filtered using a vacuum pump. The extract was concentrated under reduced pressure using a vacuum concentrator to obtain 60 g of 60 brix concentrate, and additionally, lyophilized to obtain 60 g of the extract of Ogapi powder.

2. Preparation of Gardenia Extract

The gardenia was washed with water and dried in the shade. 100 g of fine dried gardenia was added to a 3L-extraction container, and 1L of purified water was added thereto, followed by extraction of hot water at 100 ° C for 3 hours. After repeating the above procedure twice, the resulting solution was cooled to 60 ° C. and filtered using a vacuum pump. The extract was concentrated under reduced pressure using a vacuum concentrator to give 20 g of 20brix concentrate, and additionally, lyophilized to obtain 5 g of powdered gardenia extract.

Example 2: Preparation of Arthritis-Reducing Dietary Medicine Formulations

The arthritis improving composition comprising glucosamine salt, chondroitin sulfate, collagen, and vitamins as a main composition was prepared in the extracts of Ogapi and Gardenia extract from Example 1 or powder.

Each component and its content used to prepare the arthritis improving composition by the method as described above are as follows.

Hereinafter, an example of the preparation of the functional food composition will be described, but the present invention is only intended to be described in detail and not intended to limit the same.

Formulation Example 1 Preparation of Pharmaceutical Formulation

1. Preparation of powder

Prickly Pear Extract ...... 400mg

Gardenia extract ... 148 mg

Glucosamine Salt ... 200mg

Chondroitin Sulfate ...... 140mg

Collagen ...... 100mg

Vitamin C ...... 10mg

Vitamin A ...... 1mg

Vitamin E ...... 1mg

The above ingredients were mixed and filled in airtight cloth to prepare a powder.

2. Preparation of Tablets

Prickly Pear Extract ...... 200mg

Gardenia extract ...... 74 mg

Glucosamine Salt ... 600mg

Chondroitin Sulfate ...... 70mg

Collagen ...... 50mg

Vitamin C ...... 5mg

Vitamin A ...... 0.5mg

Vitamin E ...... 0.5mg

After mixing the above components, tablets were prepared by tableting according to a conventional method for producing tablets.

3. Preparation of Capsule

Prickly Pear Extract ...... 200mg

Gardenia extract ...... 74 mg

Glucosamine Salt ... 600mg

Chondroitin Sulfate ...... 70mg

Collagen ...... 50mg

Vitamin C ...... 5mg

Vitamin A ...... 0.5mg

Vitamin E ...... 0.5mg

Magnesium stearate ...

According to a conventional capsule preparation method, the above ingredients are mixed and filled into capsules to prepare a capsule.

4. Manufacture of liquid

Prickly Pear Extract ...... 200mg

Gardenia extract ...... 70 mg

Glucosamine Salt ... 2500mg

Chondroitin Sulfate ...... 200mg

Collagen ...... 100mg

Vitamin C ...... 50mg

Vitamin A ...... 5mg

Vitamin E ...... 5mg

Aspartame ...... 10mg

Purified water ...... 6.86ml

After mixing the above components according to the conventional healthy beverage production method, and then stirred and heated at 85 ℃ for about 1 hour, the resulting solution is filtered and obtained in a sterilized container, sealed sterilization and then packaged in a small packaging container to prepare a liquid Prepared.

Example 3: Efficacy test of arthritis improving food drug preparation

In the following, all experiments were performed at least three times, and each trial was performed at least three independent observations. Statistical analysis was performed by analysis of variance (ANOVA) and Student's t-test.

The RAW 264.7 macrophage line used for this was purchased from ATCC of America (American Type Culture Collection, TIB71, Maryland, USA).

The rodent macrophage line RAW 264.7 is 1 × 10 in DMEM supplemented with 10% heat inactivated FBS, feticillin G (100 IU / ml), streptomycin (100 μg / ml) and L-glutamine (2 mM). Cultures were incubated in a 37 ° C. incubator in wet conditions containing 5% CO ₂ and 95% air while maintaining ⁶ cells / ml. The negative control group was treated with only solvent DMSO, and the positive control group was treated with LPS at a concentration of 100 ng / ml to induce nitric oxide in the RAW 264.7 macrophage line. Then incubated for 20 hours.

Experimental Example 1: Nitrile oxide (NO) glass inhibitory efficacy search

Accumulated nitrite (NO) and oxidized NO were measured in the culture medium by Griess reaction.

First, 100 μl cell culture medium was mixed with 100 μl Griess reagent (1% sulfanilamide, 0.1% naphthylethylenediamine dihydorchloride / 2.5% phosphoric acid) and then at room temperature for 10 minutes. After incubation, the absorbance was measured at 540 nm with a microreader. At this time, fresh culture medium was used as a control in all experiments, the level of nitrite in the sample was calculated from the standard nitrite curve prepared in the culture medium.

As shown in FIG. 1, NO production was significantly decreased in the RAW 264.7 macrophage line treated with LPS (100 ng / ml). In addition, it was evident that concentration reduction of NO production was suppressed by bark and gardenia extract. In particular, it was found that the NO production was lowered synergistically when each treatment was performed in combination with the thorny goose (ASE) and gardenia extract (GJE).

Experimental Example 2: Search for TNF-α free inhibitory effect

Cells (1 × 10 ⁶ cell counts / ml) were preincubated for 2 hours with spines and gardenia extracts in 24-well plates and then incubated with LPS (100 ng / ml) for 6 or 18 hours. After the suspensions were removed, TNF-α levels were quantified with an immunoassay kit according to the manufacturer's protocol.

To determine if prickly pear and gardenia extracts regulate the production of inflammatory cytokine, production of TNF-α was measured using the ELISA method in RAW 264.7 macrophage line stimulated with LPS.

As shown in FIG. 2, production of TNF-α was significantly decreased in RAW 264.7 macrophage line treated with LPS (100 ng / ml). In addition, it was evident that the decrease in production of TNF-α was inhibited concentration-dependently by thorny stem (ASE) and gardenia extract (GJE). In particular, it was found that the production of TNF-α was synergistically lowered in each treatment alone when co-processing ASE and Gardenia extract (GJE).

Experimental Example 3: Analgesic inhibitory effect test

In order to confirm the analgesic inhibitory effect of the composition prepared according to Example 2, prickly pear and gardenia extract, the symptoms of writting by acetic acid of Whittle (1957) were observed in ICR mice.

To the mice, oral administration of 100 mg and 500 mg / kg of the body weight of each mouse in the composition prepared according to Example 2, the extract of Gardenia jasminoides and the composition according to Example 2, respectively, 0.1 ml / 10g of 0.7% acetic acid-physiological saline solution after 30 minutes Intraperitoneally. The number of guiding symptoms was observed from 10 minutes after injection to 10 minutes. The results are shown in Table 1.

Dose (mg / kg) Average number of rides % Inhibition Control - 20 ± 1.2 - Prickly Pear Extract 100 17 ± 1.4 15 500 15 ± 1.1 25 Gardenia Extract 100 15 ± 1.5 25 500 11 ± 1.2 45 Example 2 (Liquid Composition) 100 13 ± 1.5 35 500 9 ± 1.1 55

As shown in Table 1, the analgesic inhibitory effect was shown in all experimental groups as in the analgesic inhibitory effect, and in particular, the administration of gardenia extract 500 mg / kg and the liquid composition administered group of Example 2 was about 25-55% analgesic inhibitory effect compared to the control group. Was found to be excellent.

Experimental Example 4: Acute Toxicity Test of Food and Drug Composition

All natural products and food compositions used in the present invention are already classified as food ingredients, so it is judged that there is no problem in the stability of the herbal extracts, but the acute toxicity test was conducted as follows to check the stability during oral administration of the composition. .

As a test animal, acute toxicity test was performed for 1 week using 5 weeks old ICR mice. Ten animals per group were orally administered at a dose of 5000 mg / kg to mice fasted for 15 hours by dissolving the powder composition in the pharmaceutical composition of the present invention in 0.9% NaCl. After administration of the test substance, the mortality, clinical symptoms, and weight changes of the animals were observed and necropsied, and the abdominal and thoracic organ abnormalities were visually observed.

As a result of the test, there was no specific clinical symptoms or dead animals in all animals to which the test substance was administered, and no toxic change was observed in weight change, autopsy findings, etc. The composition including the extract of the present invention was considered safe. It became.

Experimental Example 5: Simple Clinical Test of a Food and Drug Composition

Since all natural products and food compositions used in the present invention are harmless to the human body as raw materials that are already approved for use as food raw materials by the Korea Food and Drug Administration, 40 to verify the efficacy of improving arthritis for the liquid composition prepared in Example 2 above. Twenty adults in their 60s were divided into groups administered with the composition of the present invention and placebo-administered groups, and consumed for 10 days after breakfast once a day. Experimental method was carried out by double blind method. The survey items were focused on alleviating joint pain and alleviating arthritis. Specifically, the results were measured by numerically answering questions about the degree of relief of joint pain and the degree of swelling of the joint area.

The quantification method has no effect on the survey; 20%, not very effective; 40%, moderate; 60%, with little effect; 80%, very effective; 100%.

Table 2 shows the results of the liquid composition administration group of Example 2 and Table 3 shows the results of the placebo administration group.

Example 2 (Liquid Composition Administration Group) Pain relief effect (%) Swelling effect of joint area (%) One 80 80 2 100 80 3 100 80 4 80 100 5 60 80 6 100 80 7 80 100 8 80 80 9 100 80 10 80 80 Average 86 84

Placebo group Pain relief effect (%) Swelling effect of joint area (%) 11 40 40 12 20 40 13 40 40 14 20 20 15 60 40 16 20 20 17 40 40 18 40 40 19 20 20 20 20 40 Average 32 34

As shown in Table 2 and 3, the pain relief effect and joint swelling relief effect of the composition of the present invention was confirmed that the effect is excellent by more than 80% or more in the placebo administration group 36% as no effect It was confirmed that the test result was significant. Therefore, it can be seen that the composition of the pharmaceutical composition of the present invention, including thorn stem and gardenia extract has a significant effect on the arthritis improvement effect.

As described above, according to the present invention, since it has an excellent effect of alleviating arthritis pain relief and alleviating joint swelling, it can be used as a therapeutic or preventive agent for arthritis, and can also be widely used as a health food.

As described through the Examples and Experimental Examples, the present invention relates to a composition for preventing and treating arthritis containing natural herbal extracts as an active ingredient, thorny stem and gardenia extract concentrate or powder is one of the causes of arthritis Inhibition and pain relief effect was excellent, and when administration of the composition containing thorn stem extract and gardenia extract concentrate or powder as an active ingredient, there was an excellent effect showing a remarkable arthritis pain relief effect and relieve joint swelling effect compared to the placebo control group . Therefore, the present invention can be used as a therapeutic or prophylactic agent for arthritis, and can be widely used as a health food, which is a very useful invention in the functional health food industry.

Claims (9)

A composition for the prevention and treatment of arthritis, which contains the extract of Prickly Ogapi and the extract of Gardenia vulgaris as an active ingredient. The method of claim 1, wherein the extract of the thorny scabies and the extract of gardenia is a composition for preventing and treating arthritis, characterized in that the hot water extract in the water, alcohol or a mixture of water and alcohol of the thorns. The method of claim 2, wherein the extract of the thorny opiaceae and the extract of Gardenia jasminoides extract and concentrate the filtered and concentrated to extract the hot water, or a composition for the prevention and treatment of arthritis, characterized in that the lyophilized powder form. The method according to claim 1, wherein 0.5 to 30.0% by weight of barb extract, 0.1 to 20% by weight of gardenia extract, 5 to 90% by weight of glucosamine salt, 0.1 to 5.0% by weight of chondroitin, 0.1 to 10.0% by weight of collagen, 0.01 ~ 1.0% by weight of vitamin C, 0.05 ~ 0.1% by weight of vitamin A, 0.01 ~ 0.1% by weight of vitamin E composition for preventing and treating arthritis. According to claim 1, wherein the arthritis is a composition for preventing and treating arthritis, characterized in that rheumatoid arthritis or degenerative arthritis. The composition for preventing and treating arthritis according to claim 1, wherein the composition is used as a health beverage or a beverage additive. The composition for preventing and treating arthritis according to claim 1, wherein the composition is in the form of a powder, a tablet, a capsule, or a liquid. A method of preparing a composition for preventing and treating arthritis, which comprises the following extracts of Gossipica and Gardenia extract concentrate or powder as an active ingredient comprising the following steps: Selecting and washing the barb or gardenia; Drying and washing or grinding the washed thorns or gardenia in the shade; 1 to 30 times of water, alcohol, or a mixed solution of water and alcohol per 1 kg by weight of the shredded or ground thorns or gardenia is added 1 to 10 times at 60 to 120 ° C. for 1 hour to 2 days Repeatedly extracting hot water to obtain a crude extract; And, Obtaining the crude extract 20 ~ 60 Brix extract concentrate through filtration, darkening or vacuum concentration, or lyophilizing the crude extract to obtain a powder form. The method of claim 8, wherein in the step of obtaining the crude extract for 1 to 5 hours at 80 ~ 110 ℃, repeating the hot water extract, characterized in that the extract and the gardenia extract concentrate or powder as an active ingredient, characterized in that 2 to 5 times Method for producing a composition for preventing and treating arthritis.

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