KR20080090745A - A cosmetic composition comprising an extract of vitex rotundifolia l. having antioxidative effect - Google Patents

Abstract

An extract of Vitex rotundifolia L. is provided to have high polyphenol content and show excellent electron donating ability, SOD-like activity, nitrite scavenging capability, xanthine oxidase inhibitory ability and tyrosinase inhibitory ability, thereby being usefully used as a skin aging preventing and skin whitening cosmetic composition. A cosmetic composition for preventing skin aging and whitening comprises an extract of Vitex rotundifolia L. as an effective ingredient, wherein the extract is obtained by extracting seeds or stems with water, C1-4 alcohol, a mixture of water and C1-4 alcohol, propylene glycol, ethyl acetate, butylene glycol, ether or chloroform. Further, the cosmetic composition for preventing skin aging and whitening is a form selected from cosmetic water(skin), cream, emulsion, essence, pack, spray and foundation.

Description

 A cosmetic composition comprising an extract of Vitex rotundifolia L. having antioxidative effect

       1 is a diagram showing the total polyphenol content of the extract of nabigi,

       Figure 2 is a diagram showing the electron donating ability of the naiveum extract,

       Figure 3 is a diagram showing the SOD-like activity of the naiveum extract,

      Figure 4 is a diagram showing the nitrite scavenging ability of the naiveum extract in the condition of PH 1.2,

      Figure 5 is a diagram showing the nitrite scavenging ability of the naiveum extract in the condition of PH 3.0,

      Figure 6 is a view showing the nitrite scavenging ability of the naiveum extract in the condition of PH 6.0,

      Figure 7 is a diagram showing the xanthine oxidase inhibitory activity of the extract of hibiscus sinensis,

      Figure 8 is a diagram showing the tyrosinase (tyrosinase) inhibitory ability of the extract of nabigi.

      The present invention relates to a cosmetic composition for preventing skin aging and whitening containing the extract of nabi hibiscus as an active ingredient.

Recently, as interest in aging and adult diseases increases, studies are being actively conducted to prevent disease by eliminating excessively generated reactive oxygen in vivo. Free radicals occur in aerobic organisms where oxygen, which is absolutely necessary to maintain life, is an electron acceptor and a continuous biochemical reaction is carried out to supply energy. However, due to metabolic imbalances and physicochemical factors such as chemicals and pollution, hydrogen peroxide (H ₂ O ₂ ), superoxide anion (O _2- ), singlet oxygen ( ¹ O ₂ ) , is converted to the hydroxy radical (hydroxy radical, ^_ OH) a high active oxygen (reactive oxygen) such as reactive. These free radicals cause fatal oxygen toxicity due to strong oxidative force, causing cell membrane destruction, enzyme inactivation, lipid oxidation, DNA denaturation, and cell aging, leading to serious diseases such as cancer, brain diseases, stroke, arteriosclerosis, inflammation and autoimmune diseases. Cause physiological disorders (Hammond et. al ., J. Physion . Pharmacol . 63, pp 173-187, 1985).

Currently, active research is being conducted to discover and develop useful substances for the treatment of antioxidants and antioxidants to remove free radicals. Various studies have been made to develop antioxidants from compounds such as In addition, the development of antioxidants and physiologically active substances that are useful in herbal medicine mainly used in oriental medicine (Nam and Kang, J. Food) Sci . Technol ., 36 , pp 338-338, 2000). Bioactive substances are high value-added substances that show remarkable activity in very small amounts, and many kinds are usefully used, and new substances are being actively researched (Kang et. al ., Natural product science . Seoul Univ . Publishers , Seoul, p71, 1988). Most of the bioactive substances present in natural products are phenolic compounds and are one of the secondary metabolites of various structures and molecular weights. These phenolic compounds include flavonoids, simple phenols, phenolic acids, and phenylprophanoids, and are antibacterial, anti-allergic, antioxidant, anticancer, and decayed. It is reported to be effective in prevention, heart disease and diabetes prevention (Azuma, et. al ., J. Agric . Food Chem ., 47, pp 3963-3966, 1999). Phenolic antioxidants are also known to inhibit oxidation by removing hydrogen by donating hydrogen to alkyl radicals or alkylperoxy radicals during the chain reaction (Labuza, CRC). Rev. Food Technol ., 2, p335, 1973)

Natural antioxidants known to date include tocopherols, flavonoids, gossypol, sesamol, oryzanol and vitamin C (Pszcczola DE, Food Tech). ., 55, pp51-59, 2001) . Among them, tocopherol and L-ascorbic acid are preferred as natural antioxidants. Among them, tocopherol has high safety but low ability to stop oxidation reaction (Halliwell et. al ., FASEB J. , 2 , pp2867-2870, 1988).

Vitex , the material of this experiment rotundifolia L.) belongs to Verbenaceae and is a deciduous shrub native to sub-Hwanghae Island or coastal waters and is known to grow in China and Japan (Yeeh et. al ., Plant Research , 109, pp 161-168, 1996). In oriental medicine, dried fruit of naivea japonica is used as a herbal medicine, and it is used for tonic, soothing, analgesic, anti-inflammatory, cold, eczema, tinnitus, hearing loss, vision disorder, headache, neuralgia and It is used for the treatment of various allergic diseases (Kimura et. al ., World Science . Singapore . pp141-142, 1996; Jang et al ., J. Korean Soc . Agric . Chem . Biotechnol ., 45 , pp 101-107, 2002).

Jang et 2002 al ., J. Korean Soc . Agric . Chem . Biotechnol., 45, pp101-107, 2002 ) , such as alpha pinene the stem (α -pinene) and remove the essential oil component of the 64 species, including the alpha-terpineol (α -terpineol), one can identify the bar. In addition, five types of phenolic compounds and four types of flavonoids, which show plant growth regulation, were reported (Yoshioka et. al ., Z. Naturforsch ., 59 , pp, 510-514, 2004).

     Herbal herbal medicine has been prescribed and used for the total treatment or prevention of related diseases, but its utilization is relatively low because it is not known about the active ingredient, toxicity and its efficacy. However, in Korea, there are folk remedies that use herbal medicines in various ways in daily life, so it is easy to develop and commercialize anti-aging and whitening cosmetics with antioxidant function by using herbal medicines. Therefore, it is necessary to provide scientific evidence of useful ingredients and efficacy for their proper use, and the exploration and development of functional materials, including herbal and natural products and active substances in their by-products, promotes efficient use of resources and public health. It is meaningful in that it can contribute to.

Accordingly, the present inventors have completed the present invention by confirming that the naiveum extract of the present invention has high polyphenol content, excellent electron donating ability, SOD-like activity, nitrite scavenging ability, xanthine oxidase inhibitory activity and tyrosinase inhibitory activity. .

It is an object of the present invention for preventing skin aging and whitening, which contains pure hibiscus extract having high polyphenol content, excellent electron donating ability, SOD-like activity, nitrite scavenging activity, xanthine oxidase inhibitory activity and tyrosinase inhibitory activity as active ingredients It is to provide cosmetics.

      In order to achieve the above object, the present invention provides a skin anti-aging and whitening cosmetics containing the extract of nabigi as an active ingredient.

Said naiveum tree extract includes extracts from the stems, seeds, leaves or roots of the naiveumii tree, preferably stems or seeds.

The extract is water containing purified water, lower alcohols having 1 to 4 carbon atoms, or a mixed solvent thereof, propylene glycol, ethyl acetate, butylene glycol, ether or chloroform, preferably water or a mixed solvent of water and ethanol, more Preferably, the extract is soluble in water or 60 to 80% ethanol.

Hereinafter, the method for obtaining the extract of the present invention will be described in detail.

The extracts of the naivea japonica of the present invention are finely ground the seeds or stems of the shaded naivea japonica, and the volume of water, ethanol, methanol, etc., which is about 1 to 40 times its weight, preferably about 1 to 30 times its weight. C ₁ to C ₄ lower alcohols or mixed solvents thereof, preferably water or 60 to 80% ethanol for about 1 to 10 hours, preferably about 2 to 5 hours, hot water extraction, ultrasonic extraction, reflux cooling, etc. The extract obtained by extraction under reduced pressure, preferably by reflux cooling extraction, was concentrated under reduced pressure at about 40 to 80 ℃, preferably about 60 to 70 ℃, and concentrated under reduced pressure, suspended in distilled water to freeze the vacuum Drying, hot air drying or drying by spraying, preferably lyophilization can be obtained in the extract of the naivea japonica of the present invention.

It provides a skin anti-aging and whitening cosmetic composition containing the pure hibiscus extract obtained by the above manufacturing process of the present invention as an active ingredient.

In addition, as a medicinal herb, which has been used for a long time as a herbal medicine and food, the extracts of the present invention extracted from them also have no problems such as toxicity and side effects. Can be used.

The anti-aging and whitening cosmetic composition of the present invention, the extract contains 0.1 to 50% by weight based on the total weight of the composition.

The composition comprising the naiveum extract of the present invention can be used in various ways, such as cosmetics, face wash and shampoo for skin anti-aging or whitening effect. Examples of products to which the present composition can be added include cosmetics such as various creams, lotions, skins, and the like, shampoos, rinses, cleansing agents, face washes, soaps, treatments, and cosmetic liquids.

Cosmetics of the present invention comprises a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids and seaweed extract.

The water-soluble vitamins may be any compound that can be incorporated into cosmetics, but preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H, and the like. Their salts (thiamine hydrochloride, sodium ascorbate salt, etc.) and derivatives (ascorbic acid-2-sodium phosphate salt, ascorbic acid-2-magnesium phosphate salt, etc.) may also be used in the water-soluble vitamins that can be used in the present invention. Included. The water-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification of microorganism culture, enzyme or chemical synthesis.

The oil-soluble vitamin may be any compound that can be formulated in cosmetics, but preferably vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-α-tocopherol, d-α-tocopherol, d-δ-tocopherol) and the like. And derivatives thereof (ascorbic palmitate, ascorbic stearate, ascorbic acid dipalmitate, dl-α-tocopherol acetate, dl-α-tocopherolvitamin E nicotinic acid, DL-pantothenyl alcohol, D-pantothenyl) Alcohols, pantotenylethyl ether, etc.) are also included in the oil-soluble vitamins used in the present invention. Oil-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification of microorganism culture, enzyme or chemical synthesis.

The polymer peptide may be any compound as long as it can be incorporated into cosmetics. Preferably, collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin, and the like can be given. Polymeric peptides can be purified and obtained by conventional methods such as purification from microbial cultures, enzymatic methods or chemical synthesis methods, or can be purified and used from natural products such as dermis and pig silk such as pigs and cattle.

The polymer polysaccharide may be any compound as long as it can be blended into cosmetics. Preferably, hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.) may be mentioned. For example, chondroitin sulfate or its salt can be normally purified from a mammal or fish.

The sphingolipid may be any compound as long as it can be blended into cosmetics. Preferably, ceramide, pit spingosine, sphingolipid lipid and the like can be given. Sphingo lipids can usually be purified from mammals, fish, shellfish, yeasts or plants by conventional methods or obtained by chemical synthesis.

The seaweed extract may be any compound as long as it can be blended into cosmetics. Preferably, the seaweed extract may include brown algae extract, red algae extract, green algae extract, and the like. Also, calginine, arginic acid, sodium arginate, Potassium arginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained by purification from seaweed by conventional methods.

In addition to the said essential component, you may mix | blend the cosmetics of this invention with the other components normally mix | blended with cosmetics as needed.

Other components that may be added include fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings, Blood circulation accelerators, cooling agents, restriction agents, purified water and the like.

Examples of the fat or oil component include ester fats, hydrocarbon fats, silicone fats, fluorine fats, animal fats, and vegetable fats and oils.

As ester fats and oils, glyceryl tri2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl mystinate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, and stearic acid Butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl acid isetyl, isostyl acid mystearyl, isostaryl palmitate, octylate acid octyldodecyl, isostearic acid isetyl, diethyl sebacinate, adidi Diisopropyl phosphate, isoalkyl neopentane, tri (capryl, capric acid) glyceryl, trimethyl ethyl trimethylolpropane, trimethyl stearate trimethylol propane, tetra 2-ethylhexanopentane Tol, cetyl caprylate, lauric acid decyl, lauric acid hexyl, myristic acid decyl, myristic acid myristyl, myristic acid cetyl, stearyl stearate, decyl oleate, lisinooleic acid Teyl, isostearyl laurate, isotridecyl myristin, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyl dodecyl oleate, octyl dodecyl linoleate, isopropyl isopropyl acid, 2-ethylhexanoic acid cetostearyl, 2-ethylhexanoic acid stearyl, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicapric acid, propylene glycol di (capryl capric acid), Dicaprylic acid propylene glycol, dicapric acid neopentyl glycol, dioctanoate neopentyl glycol, tricaprylate glyceryl, triundecyl glyceryl, triisopalmitinate glyceryl, triisostearate glyceryl, octylate octadodode Sil, isostearyl octanoate, octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isstearyl isostearate, Isostearic acid octyldecyl, polyglycerol oleic acid ester, polyglycerin isostearic acid ester, triisocetyl citrate, triisoalkyl citrate, triisooctyl citrate, lauryl lactate, myritic lactic acid, cetyl lactate, octyl lactate, trit citrate Ethyl, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl malic acid, 2-ethylhexyl hydroxystearate, di2-ethyl hexyl succinate, diisobutyl adipic acid, diisopropyl sebacic acid, Dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, physteryl isostearate, phytic oleate, 12-Steloylhydroxystearate isocetyl, 12-Steloylhydroxystearate stearyl, 12-steal One hydroxy stearic acid and the like esters such as cetearyl source.

Examples of the hydrocarbon-based oils and fats include hydrocarbon oils such as squalene, liquid paraffin, α-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybutene, microcrystalline wax, and vaseline.

Examples of the silicone-based oils and fats include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane and methylcetyloxysiloxane copolymer, dimethylsiloxane and methyl steoxysiloxane copolymer and alkyl. Modified silicone oil, amino modified silicone oil and the like.

Perfluoro polyether etc. are mentioned as fluorine-based fats and oils.

Animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, soybean oil, soybean oil, corn oil, rapeseed oil, almond oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil. , Cottonseed oil, Palm oil, Cucumber nut oil, Wheat germ oil, Rice germ oil, Shea butter, Walnut colostrum oil, Marker demia nut oil, Meadow home oil, Egg yolk oil, Uji, Horse oil, Mink oil, Orange rape oil, Jojoba oil And animal or plant fats and oils such as candeler wax, carnava wax, liquid lanolin and hardened castor oil.

Examples of the moisturizing agent include a water-soluble low molecular moisturizer, a fat-soluble molecular moisturizer, a water-soluble polymer, and a fat-soluble polymer.

Water-soluble low molecular humectants include serine, glutamine, sorbitol, mannitol, pyrrolidone-sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol (polymerization degree n = 2 or more), polypropylene glycol (Polymerization degree n = 2 or more), polyglycerol (polymerization degree n = 2 or more), lactic acid, lactic acid salt, etc. are mentioned.

Examples of the fat-soluble low molecular humectants include cholesterol and cholesterol esters.

Examples of the water-soluble polymer include carboxyvinyl polymer, polyasparaginate, tragacanth, xanthan gum, methyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, water soluble chitin, chitosan, and dextrin. Can be.

Examples of the fat-soluble polymers include polyvinylpyrrolidone-eicosene copolymers, polyvinylpyrrolidone-hexadecene copolymers, nitrocellulose, dextrin fatty acid esters, polymer silicones, and the like.

Examples of the emollient include long-chain acyl glutamic acid cholesteryl esters, hydroxy stearic acid cholesterol, 12-hydroxystearic acid, stearic acid, rosin acid, lanolin fatty acid cholesteryl esters, and the like.

As surfactant, nonionic surfactant, anionic surfactant, cationic surfactant, amphoteric surfactant, etc. are mentioned.

Nonionic surfactants include self-emulsifying glycerin monostearate, propylene glycol fatty acid esters, glycerin fatty acid esters, polyglycerol fatty acid esters, sorbitan fatty acid esters, POE (polyoxyethylene) sorbitan fatty acid esters, POE sorbitan fatty acid esters, POE Glycerin Fatty Acid Ester, POE Alkyl Ether, POE Fatty Acid Ester, POE Cured Castor Oil, POE Castor Oil, POE · POP (Polyoxyethylene Polyoxypropylene) Copolymer, POE / POP Alkyl Ether, Polyether Modified Silicone Alkanolamide, alkylamine oxide, hydrogenated soybean phospholipid, etc. are mentioned.

As anionic surfactant, fatty acid soap, (alpha)-acyl sulfonate, alkyl sulfonate, alkyl allyl sulfonate, alkyl naphthalene sulfonate, alkyl sulfate, POE alkyl ether sulfate, alkylamide sulfate, alkyl phosphate, POE alkyl phosphorus salt, alkylamide Phosphates, alkyloylalkyltaurine salts, N-acylamino acid salts, POE alkyl ether carboxylate salts, alkyl sulfosuccinate salts, sodium alkyl sulfo acetates, acylated hydrolyzed collagen peptide salts, perfluoroalkyl phosphate esters, and the like. have.

As cationic surfactant, alkyl trimethylammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium chloride, cetostearyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyl dimethyl benzyl ammonium bromide, behenyl trimethyl ammonium chloride, chloride Benzalkonium, diethylaminoethyl stearate, dimethylaminopropyl stearate, lanolin derivatives, quaternary ammonium salts, and the like.

Examples of amphoteric surfactants include carboxybetaine type, amidebetaine type, sulfobetaine type, hydroxysulfobetaine type, amide sulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type and amideamine type. An amphoteric surfactant etc. are mentioned.

Organic and inorganic pigments include silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, bengala, clay, bentonite, titanium film mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, aluminum oxide Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine blue, chromium oxide, chromium hydroxide, calamine, carbon black and composites thereof; Polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluorine resin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzene, styrene copolymer, Organic pigments such as silk powder, cellulose, CI pigment yellow, CI pigment orange, and composite pigments of these inorganic pigments and organic pigments;

As organic powder, Metal soaps, such as a calcium stearate; Alkyl phosphate metal salts such as sodium cetylinate, zinc lauryl acid and calcium laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-β-alanine calcium, N-lauroyl-β-alanine zinc, and N-lauroylglycine calcium; Amide sulfonic acid polyvalent metal salts, such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N-acyl basic amino acids, such as N (epsilon) -lauroyl-L- lysine, N (epsilon) -palmitolysine, N (alpha)-partoylol nitin, N (alpha)-lauroyl arginine, and N (alpha) -cured fatty acid acyl arginine; N-acyl polypeptides, such as N-lauroyl glycyl glycine; α-amino fatty acids such as α-aminocaprylic acid and α-aminolauric acid; Polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride and the like.

Examples of the ultraviolet absorber include paraaminobenzoic acid, ethyl paraaminobenzoate, amyl paraaminobenzoic acid, octyl paraaminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomentyl salicylate and cinnamic acid benzyl , Paramethoxy cinnamic acid 2-ethoxyethyl, paramethoxy cinnamic acid octyl, diparamethoxy cinnamic acid mono 2-ethylhexane glyceryl, paramethoxy cinnamic acid isopropyl, diisopropyl diisopropyl cinnamic acid ester mixture, urokanoic acid Ethyl urocanate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and salts thereof, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenonedisulfonate, dihydroxybenzophenone, tetra Hydroxybenzophenone, 4- tert -butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino- p- (carbo-2'-ethylhexyl-1'-oxy) -1,3 , 5-triazine, 2- (2-hydric When the like can be mentioned 5-methylphenyl) benzotriazole.

As fungicides, hinokithiol, trichloric acid, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, ginxitlionone, benzalkonium chloride, photosensitive Sodium No. 301, sodium mononitroguicol, undecylenic acid, and the like.

Examples of the antioxidant include butylhydroxyanisole, propyl gallic acid, and erythorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fmaric acid, sodium pmarate, succinic acid, sodium succinate, sodium hydroxide, sodium dihydrogen phosphate, and the like.

Examples of the alcohol include higher alcohols such as cetyl alcohol.

In addition, the compounding component which may be added other than this is not limited to this, Moreover, Although all said components can be mix | blended within the range which does not impair the objective and effect of this invention, Preferably it is 0.01-5 weight% with respect to gross weight, More Preferably it is 0.01-3 weight% compounding.

The cosmetic of the present invention may take the form of a solution, an emulsion, a viscous mixture, or the like.

Ingredients included in the cosmetic composition of the present invention includes components commonly used in cosmetic compositions in addition to the extract of naiveum as an active ingredient, for example, conventional auxiliary agents such as stabilizers, solubilizers, vitamins, pigments and flavorings. And carriers.

The cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, and includes, for example, milky lotion, cream, lotion, pack, foundation, lotion, essence, hair cosmetic, and the like.

Specific examples of the cosmetic of the present invention include a flexible lotion, nourishing lotion, nourishing cream, cleansing cream, face wash, cleansing cream, cleansing milk, cleansing lotion, massage cream, cold cream, moisturizing cream, pack, aftershave cream, sunburn prevention cream Suntan oil, body shampoo, hair shampoo, hair rinse, hand cream, foundation, and the like.

When the formulation of the present invention is a paste, cream or gel, animal carriers, vegetable fibers, waxes, paraffins, starches, tracantes, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide, etc. may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating or emulsifying agent is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the dosage form of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

Below, The invention is illustrated in detail by the following examples and experimental examples.

      However, the following Examples and Experimental Examples are merely illustrative of the present invention, and the content of the present invention is not limited by the following Examples and Experimental Examples.

Reference Example  1. Preparation of Sample

After harvesting the naivea wood used in the present invention at a Korean herb production farm in Hadong, Gyeongnam around July 2006, the stems were separated from the stems, washed with distilled water, dried and finely chopped, and the seeds were sold as herbal materials. Mangja was purchased from Daegu Hannam Pharmaceutical Co., Ltd. and washed three times in distilled water to remove seedlings, and then dried at -75 ° C. to prepare seeds and stems of puree wood.

Reference Example  2. Statistical Processing

The following experimental example shows the results of experiments repeated independently three times as the average ± standard deviation. To test the significance between the experimental groups, ANOVA test was performed using SPSS 12.0 and Duncan's multiple range test was performed at the p <0.05 level.

Example  1. Water Extract of Radish Seeds ( WE -1) manufacturing

Prepared in Reference Example 1 to a round flask attached with a reflux condenser 100 ml of the seed sample was added to 1,000 mL of distilled water, and the extract obtained by extracting the extract three times for three hours in an 80 ° C water bath was filtered with a filter paper and concentrated under reduced pressure with a rotary vacuum evaporator (Eyela 400 series, Japan). After freeze-drying to prepare a powder of 4.381g (hereinafter referred to as WE-1), was used as a sample of the following experimental example.

Example  2. Water Extracts of Stem Bark Stem ( WE -2) manufacturing

The same procedure was followed except that the seed sample of Example 1 was changed to 100 g of stem sample, thereby preparing 9.013 g of water extract of puree ivy stem (hereinafter, referred to as WE-2), and as a sample of the following experimental example. Used.

Example  3. 70% Ethanol Extract of Radish Seeds EE -1) manufacturing

Prepared in Reference Example 1 to a round flask attached with a reflux condenser 1,000 g of 70% ethanol was added to 100 g of the seed sample, and the extract obtained by three times of repeated extraction for 3 hours in a 60 ° C. water bath was filtered through a filter paper, and then filtered using a rotary vacuum evaporator (Eyela 400 series, Japan). Concentrated under reduced pressure and lyophilized to prepare a powder of 3.780g (hereinafter referred to as EE-1), was used as a sample of the following experimental example.

Example  4. 70% Ethanol Extract of Radish Stem ( EE -2) manufacturing

      Except for changing the seed sample of Example 3 to 100g of the stem sample was carried out the same process to prepare a 10.875g 70% ethanol extract of the stem of the naivea tree (hereinafter referred to as EE-2), the following experimental example Used as a sample.

Example  5. Seed of Beetle Tree Seeds Hydrothermal  extract( HWE -1) manufacturing

Prepared in Example 1 to a round flask attached with a reflux condenser After distilled water (1000mL) was added to 100g seed sample, the extract obtained by extracting for 3 hours under pressure of 110 ℃ and 1.5 atm using a pressure extractor was filtered with filter paper and concentrated under reduced pressure with a rotary vacuum evaporator (Eyela 400 series, Japan). It was lyophilized to prepare 9.388 g of powder (hereinafter referred to as HWE-1), and used as a sample of the following experimental example.

Example  6. of naive tree trunk Hydrothermal  extract( HWE -2) manufacturing

       Except for changing the seed sample of Example 5 to 100g of the stem sample was carried out in the same process to prepare 14.425g of hydrothermal extract of the naivea stem (hereinafter referred to as HWE-2), the sample of the following Experimental Example Used.

Experimental Example  1. Measurement of polyphenol content

As described in the existing literature to determine the polyphenol content of the naiveum extract (WE-1, WE-2, EE-1, EE-2, HWE-1, HWE-2) prepared in Examples 1 to 6 The experiment was performed using the method as follows (AOAC; Association of official analytical chemists, Washington DC USA , 45 , pp 21-22, 2005).

Each of the extracts of the naivea japonica prepared in Examples 1 to 6 (WE-1, WE-2, EE-1, EE-2, HWE-1, HWE-2) in secondary distilled water at a concentration of 1 mg / mL After dilution, add 0.2 mL of Folin-ciocalteu's phenol reagent, mix, and leave at room temperature for 3 minutes, then add 0.4 mL of saturated solution of sodium carbonate (Na ₂ CO ₃ ) It was. 1.4 mL of distilled water was added thereto, reacted at room temperature for 1 hour, and the absorbance was measured at 725 nm using a UV / VIS spectrophotometer. Taking a final concentration of polyphenol compounds 0, 37.5, 75, 150, burnt dystocia (tannic acid) so that the 300μg / mL from the standard curve of the absorbance measured above morpholine-Denis (AOAC; Association of official analytical chemists, Washington DC USA , 45 , pp21-22, 2005) was used to determine the total polyphenol compound content of the extracts of naivea quince, and the results are shown in Table 1 and FIG.

extract Total Polyphenols (mg / g) extract Total Polyphenols (mg / g) Seed dry   6.08 ± 0.07 Stem dry   8.08 ± 0.05 WE-1 of Example 1 186.69 ± 0.90 WE-2 of Example 2 122.01 ± 0.90 EE-1 of Example 3 100.80 ± 1.55 EE-2 of Example 4 176.34 ± 3.23 HWE-1 of Example 5 182.55 ± 0.90 HWE-2 of Example 6 171.17 ± 2.37

      As a result of the experiment, as shown in Table 1, the dried stem contained about 8.08 mg / g of polyphenol, the dried seed contained about 6.08 mg / g, the WE-1 of Example 1 in the extract It contained the most polyphenols at about 186.69 mg / mL. In addition, the HWE-1 of Example 5 and the HWE-2 of Example 6 were about 182.55 mg / g and 171.17 mg / g, respectively, and thus the extraction was well performed because the bonds with tissues were destroyed at high temperature and high pressure of 100 ° C. or higher. Polyphenol eluted. Therefore, it can be seen that the seeds and stems of the hibiscus paniculata contain a large amount of polyphenols, which are known as the main substances exhibiting antioxidant activity, and thus have excellent effects as natural antioxidants.

Experimental Example  2. Electron donating ability  Measure

In order to determine the antioxidant activity of the extract of S. aureus, it was blown using DPPH ( 1,1-diphenyl-2-picryl hydrazyl), which can determine the antioxidant activity according to the degree of reduction of the bioactive substance and the discoloration of purple. According to the method of Blois, the electron donating ability of the extract of naivea was measured.

       2 mL each of the extracts of the naivea japonica (WE-1, WE-2, EE-1, EE-2, HWE-1, HWE-2) prepared in Examples 1 to 6 at a concentration of 0.1, 0.5 or 1.0 mg / mL 1mL of 0.2 mM DPPH solution dissolved in 99% ethanol was added thereto, followed by mixing for 30 minutes at 37 ° C. The reaction solution was measured for absorbance at 517 nm, and the electron donating ability was obtained by expressing the difference in absorbance before and after sample addition as a percentage (%), and the results are shown in Table 2 and FIG. 2.

Concentration (mg / mL) Electron Donating Ability of Extract WE-1 of Example 1 EE-1 of Example 3 HWE-1 of Example 5 WE-2 of Example 2 EE-2 of Example 4 HWE-2 of Example 6 0.1 88.40 ± 0.35 ^cd 90.32 ± 0.09 ^b 88.03 ± 1.11 ^d 90.93 ± 0.18 ^ab 91.61 ± 0.51 ^a 89.06 ± 0.33 ^c 0.5 91.21 ± 0.16 ^c 94.66 ± 0.00 ^a 90.35 ± 0.28 ^c 92.30 ± 0.09 ^b 94.43 ± 0.00 ^a 92.68 ± 1.26 ^b 1.0 95.08 ± 0.40 ^c 95.97 ± 0.33 ^b 91.14 ± 0.42 ^e 93.46 ± 0.09 ^d 96.92 ± 0.33 ^a 94.59 ± 0.48 ^c

      As a result, as shown in Table 2, the EE-1 of Example 3 and EE-2 of Example 4 at the concentration of 1.0mg / mL showed the highest electron donating ability of about 95.97% and 96.92%, respectively, All extracts showed more than 88% of the activity, and the electron donating effect increased significantly as the concentration of the sample increased. Therefore, the seed and stem extracts of S. aureus were found to be herbal medicine resources with very high electron donating ability.

Experimental Example  3. SOD Pseudo-activity  Measure

      In order to determine the degree of antioxidant activity of the extract of hibiscus sinensis, experiments were performed using the SOD-like activity measurement method related to antioxidant and anti-aging activity.

Pyrogallol, an oxidase that catalyzes the conversion of hydrogen peroxide (H ₂ O ₂ ) according to the method of Marklund and Marklund, Eur . J. Biochem . , 47, pp468-474, 1975. ) SOD-like activity was measured by measuring the amount of production. Each of the extracts of the naivea japonica (WE-1, WE-2, EE-1, EE-2, HWE-1, HWE-2) prepared in Examples 1 to 6 at a concentration of 0.1, 0.5 or 1.0 mg / mL To the mL, add 2.6 mL of tris-HCl buffer, 50 mM tris [hydroxymethyl] amino-methane + 10 mM EDTA, and 0.2 mL of 7.2 mM pyrogallol, adjusted to pH 8.5, for 10 minutes at 25 ° C. After the reaction, 0.1 mL of 1 N hydrochloric acid (HCl) was added to stop the reaction. The amount of oxidized pyrogallol in the reaction solution measured the absorbance at 420 nm, and the difference in absorbance between the addition group and the no addition group of the extract of S. aureus was expressed as a percentage (%), and the results are shown in Table 3 and FIG. 3. Indicated.

Concentration (mg / mL) SOD-like Activity of Extract WE-1 of Example 1 EE-1 of Example 3 HWE-1 of Example 5 WE-2 of Example 2 EE-2 of Example 4 HWE-2 of Example 6 0.1 20.99 ± 0.23 ^a 0.67 ± 0.12 ^d 5.10 ± 1.17 ^c 6.27 ± 0.79 ^b 0.82 ± 0.00 ^d 0.41 ± 0.00 ^c 0.5 46.48 ± 0.89 ^a 11.02 ± 1.66 ^d 34.38 ± 1.77 ^b 28.76 ± 2.01 ^c 5.74 ± 1.08 ^e 2.05 ± 0.74 ^f 1.0 56.50 ± 0.42 ^b 12.48 ± 0.61 ^d 83.40 ± 1.06 ^a 47.32 ± 0.45 ^c 8.34 ± 0.56 ^e 6.01 ± 1.03 ^f

       As a result, as shown in Table 3, HWE-1 of Example 5 (83.40%)> WE-1 of Example 1 (56.50%)> WE-2 of Example 2 at a concentration of 1.0 mg / mL 47.32%)> EE-1 of Example 3 (12.48%)> EE-2 of Example 4 (8.34%)> HWE-2 of Example 6 (6.01%) in order of increasing sample concentration As a result, SOD-like activity increased significantly. Accordingly, it can be seen that the extract of S. aureus has an excellent effect on anti-oxidation and aging by having high SOD activity.

Experimental Example  4. Nitrite Scavenging power  Measure

By Vitex extract remove nitrite (NaNO ₂₎ to easily create nitrosamines (nitrosamine) is carcinogenic to see if involved in antioxidant Kato (Kato et al ., Agric . Biol . Chem ., 51 , pp1333-1338, 1987), the nitrite (NaNO ₂ ) removal was measured according to the method.

Each of the extracts of the naive hibiscus (WE-1, WE-2, EE-1, EE-) prepared in Examples 1 to 6 at a concentration of 0.1, 0.5 or 1.0 mg / mL in 2 mL of 1 mM nitrite (NaNO ₂ ) solution. 2, HWE-1, HWE-2) and adjust the pH of the reaction solution to 1.2, 3.0 or 6.0 using 0.1 N hydrochloric acid (HCI, pH 1.2) and 0.2 M citric acid buffer, respectively. After that, the reaction solution was made to have a volume of 10 mL and reacted at 37 ° C. for 1 hour, followed by 1 mL of each. To this was added 5 mL of 2% acetic acid and the grease reagent (A: B = 1: 1, A; 1% sulfanilic acid in 30% acetic acid, B; 1% naphthylamine in 30% acetic acid) 0.4mL was added and mixed, followed by reaction at room temperature for 15 minutes. The absorbed sample was measured at 520 nm, and the control group was measured in the same manner as above by adding 0.4 mL of distilled water instead of the grease reagent, and the percentage difference in absorbance between the addition group and the no addition group of puree wood extract. (%), And the results are shown in Table 4 and FIGS. 4 to 6.

Concentration (mg / mL) Nitrite Scavenging Activity of Extracts WE-1 of Example 1 EE-1 of Example 3 HWE-1 of Example 5 WE-2 of Example 2 EE-2 of Example 4 HWE-2 of Example 6 pH 1.2 0.1 1.10 ± 0.23 ^d 27.21 ± 0.44 ^a 6.21 ± 0.67 ^c 19.32 ± 0.39 ^b 0.83 ± 0.42 ^d 27.34 ± 0.72 ^a 0.3 19.77 ± 1.37 ^d 50.82 ± 0.67 ^a 24.10 ± 0.56 ^c 46.73 ± 0.37 ^b 17.70 ± 0.12 ^e 51.37 ± 0.23 ^a 0.5 32.77 ± 1.59 ^e 70.01 ± 0.47 ^b 34.98 ± 1.35 ^d 64.86 ± 0.19 ^c 32.09 ± 0.32 ^e 72.19 ± 1.17 ^a 1.0 59.30 ± 0.71 ^d 86.61 ± 0.58 ^b 59.27 ± 2.36 ^d 84.61 ± 0.28 ^c 54.75 ± 0.55 ^e 88.36 ± 0.83 ^a pH 3.0 0.1 8.00 ± 0.96 ^d 12.88 ± 0.60 ^b 9.93 ± 0.78 ^c 12.95 ± 0.68 ^b 6.67 ± 0.51 ^e 16.75 ± 0.52 ^a 0.3 17.98 ± 0.17 ^b 18.23 ± 0.34 ^b 21.24 ± 0.50 ^a 18.17 ± 0.26 ^b 12.92 ± 0.51 ^c 21.21 ± 0.43 ^a 0.5 24.46 ± 1.24 ^b 21.41 ± 0.73 ^c 26.35 ± 1.01 ^a 22.11 ± 0.27 ^c 16.40 ± 0.41 ^d 23.84 ± 0.91 ^b 1.0 35.73 ± 0.82 ^a 26.53 ± 0.59 ^d 34.30 ± 0.56 ^b 25.83 ± 0.26 ^d 26.43 ± 0.43 ^d 30.24 ± 0.52 ^c pH 6.0 0.1 0.63 ± 0.60 ^d 2.29 ± 0.59 ^b 0.28 ± 0.48 ^d 3.53 ± 0.46 ^a 1.21 ± 0.62 ^c 2.00 ± 0.20 ^b 0.3 6.51 ± 0.46 ^a 7.52 ± 0.49 ^a 7.12 ± 0.68 ^a 4.97 ± 0.65 ^b 5.01 ± 0.62 ^b 6.50 ± 0.38 ^a 0.5 10.79 ± 0.17 ^ab 10.52 ± 0.42 ^ab 11.13 ± 0.43 ^a 7.02 ± 1.01 ^d 9.73 ± 0.61 ^b 8.63 ± 0.54 ^c 1.0 18.78 ± 0.60 ^a 14.07 ± 0.36 ^c 19.55 ± 0.43 ^a 10.32 ± 1.37 ^d 16.12 ± 0.70 ^b 13.59 ± 0.23 ^c

       As a result, as shown in Table 4, at pH 1.2, HWE-2 of Example 6 exhibited the highest nitrite scavenging effect at about 88.36%, and at pH 3.0, WE-1 of Example 1 was about 35.73%. The highest effect was shown, and at pH 6.0, HWE-1 of Example 5 showed the highest effect at about 19.55%. Accordingly, it was confirmed that the lower the pH, the higher the sample concentration, the higher the nitrite scavenging effect, and the higher the pH, the greater the nitrite scavenging effect of the seed than the stem. Therefore, the seeds and stems of pure hibiscus tree, which are used as herbal medicines, have excellent nitrite scavenging effect, and have an excellent effect on inhibiting nitrosamine production and antioxidant activity when ingested or processed together with products containing nitrite and amine. It was found out.

Experimental Example  5. Xanthine Oxidase Inhibition  Measure

Xanthine oxidase oxidizes xanthine into uric acid, which is involved in purine metabolism in vivo and causes gout and kidney disease (Kramer and Curhan, American). Journal of Kidney diseases, 40 , pp37-42, 2002), to determine whether the extracts of the ivy are involved in the inhibition of these xanthine oxidases, Stirpe, Corte, Biol . Chem ., 244 , pp 3855-3861, 1969) was carried out to measure the xanthine oxidase inhibitory activity.

      Pure Beetle extracts prepared in Examples 1 to 6 diluted at a concentration of 0.1, 0.5 or 1.0 mg / mL (WE-1, WE-2, EE-1, EE-2, HWE-1, HWE-2) ) 0.1mL potassium phosphate buffer (pH 7.5) 0.6mL and xantine 2mM dissolved in a substrate solution 0.2mL was added. 0.1 mL of xanthine oxidase (0.2 U / mL) was added thereto, followed by reaction at 37 ° C. for 5 minutes, and then 1 mL of 1N hydrochloric acid (HCl) was added to stop the reaction, followed by absorbance of the uric acid generated in the reaction solution. Measured at 292 nm. The xanthine oxidase inhibitory activity of the extract of the hibiscus japonica was determined by expressing the percentage decrease in absorbance of the sample solution addition group and the no addition group as a percentage (%), and the results are shown in Table 5 and FIG. 7.

Concentration (mg / mL) Xanthine Oxidase Inhibitory Activity of Extracts WE-1 of Example 1 EE-1 of Example 3 HWE-1 of Example 5 WE-2 of Example 2 EE-2 of Example 4 HWE-2 of Example 6 0.1 69.55 ± 1.66 ^a 47.21 ± 1.08 ^d 37.50 ± 0.94 ^e 51.13 ± 051 ^c 61.62 ± 1.19 ^b 61.11 ± 0.96 ^b 0.5 92.74 ± 1.59 ^ab 94.10 ± 1.38 ^a 90.76 ± 1.53 ^b 92.73 ± 0.87 ^ab 91.50 ± 0.83 ^b 92.50 ± 0.00 ^ab 1.0 94.42 ± 1.04 ^bc 97.51 ± 0.42 ^a 94.02 ± 0.65 ^c 95.24 ± 1.80 ^bc 94.40 ± 0.97 ^bc 96.11 ± 0.96 ^ab

      As a result of the experiment, as shown in Table 5, the extracts of the naivea japonica prepared in Examples 1 to 6 (WE-1, WE-2, EE-1, EE-2, HWE-1, HWE-2) The inhibitory effect of about 37.50 to 96.11%, the HWE-2 of Example 6 showed the highest inhibitory effect of xanthine oxidase by showing an inhibition rate of about 96.11%, all extracts at a concentration of 0.5 mg / mL It showed over 90% inhibitory effect. Therefore, the extract of naivea japonica prevents xanthine from being oxidized to uric acid, which is effective in preventing gout and kidney disease, and has an excellent effect as an antioxidant.

Experimental Example  6. Tyrosinase Inhibition  Measure

Synthesis of melanin due to tyrosinase has the problem of forming spots, freckles, blotch, etc., promoting skin aging and causing skin cancer, and degrading the quality due to browning of vegetables, fruits and fish (Sanchez- Ferrer et al ., Biochem . biophys . Acta ., 1247 , pp 1-11, 1995), in order to determine whether the extract of S. aureus is involved in inhibiting tyrosinase (Yagi et al ., Planta Medica 53 , pp517-519, 1987) and the degree of tyrosinase inhibitory activity was measured according to the method.

      The nets prepared in Examples 1 to 6 diluted with 0.2 mL of substrate solution dissolved in 10 mM L-DOPA in 0.5 mL of 0.175 M sodium phosphate buffer (pH 6.8) and concentrations of 0.1, 0.5 or 1.0 mg / mL. Mushroom tyrosinase (110 U / mL) in a solution containing 0.1 mL of each extract (WE-1, WE-2, EE-1, EE-2, HWE-1, HWE-2) After adding 0.2 mL and reacting at 25 ° C. for 2 minutes, the resulting DOPA chrome was measured at an absorbance of 475 nm. The tyrosinase inhibitory activity of the extract of the hibiscus japonica extract was determined by expressing the percentage decrease in absorbance of the sample solution addition group and no addition group as a percentage (%), and the results are shown in Table 6 and FIG. 8.

Concentration (mg / mL) Tyrosinase Inhibitory Activity of Extracts WE-1 of Example 1 EE-1 of Example 3 HWE-1 of Example 5 WE-2 of Example 2 EE-2 of Example 4 HWE-2 of Example 6 0.1 6.40 ± 0.40 ^a 4.98 ± 0.68 ^b 7.53 ± 1.26 ^a 7.15 ± 0.68 ^a 1.25 ± 0.00 ^b 7.65 ± 0.78 ^a 0.3 23.27 ± 0.81 ^b 14.16 ± 0.89 ^d 24.03 ± 1.07 ^b 16.33 ± 0.47 ^c 7.38 ± 0.12 ^e 31.12 ± 1.13 ^a 0.5 25.20 ± 0.40 ^d 21.13 ± 1.01 ^e 29.04 ± 0.35 ^c 35.97 ± 1.36 ^b 13.97 ± 0.29 ^a 38.90 ± 1.21 ^a 1.0 46.67 ± 0.61 ^d 29.09 ± 0.77 ^e 48.58 ± 1.07 ^c 53.47 ± 0.79 ^b 28.79 ± 0.82 ^e 57.84 ± 0.75 ^a

       As a result, as shown in Table 6, HWE-2 of Example 6 showed the highest tyrosinase inhibition rate of about 57.84% at a concentration of 1.0 mg / mL, and HWE-2 of Example 6> In the order of WE-2 of Example 2> EE-2 of Example 4, the tyrosinase inhibition rate was higher in the stems than in the seeds, and the tyrosinase inhibition rate was increased as the concentration of the sample was increased in all extracts. Accordingly, it can be seen that the extract of S. aureus has an excellent effect on skin whitening by inhibiting tyrosinase.

Experimental Example  7. Skin irritation test of naiveum extract

In order to investigate the skin irritation of the extracts of the larvae, skin irritation tests were conducted internally in New Zealand White male rabbits according to the Korea Food and Drug Administration Notice 1999-61 "Toxicological Standards of Drugs." It was. Observation of skin irritation reactions such as erythema, scalp formation, edema formation to the patch site 24 hours after the patch.

As a result of applying the extract of rabbit to the skin of rabbits, no change in general symptoms was observed, and no dead animals were observed. In addition, as a result of erythema and skin formation, light redness was found in 6 rats, but as a result of evaluation of irritation, the irritant index was classified as a weak irritant substance, which was evaluated as a substance with little irritation and can be used safely. there was.

      Examples of the formulation of the composition are described below, but are not intended to limit the present invention but to explain in detail only.

Formulation example  1. flexible lotion ( skin )

Butylene glycol, glycerin, polyoxyethylene (60) hardened castor oil, betaine, citric acid, sodium citrate, and preservatives were added to the purified water, followed by stirring and dissolving. Pear extract was added thereto, the mixture was sufficiently stirred, and then aged to prepare a pear extract-containing flexible lotion. Table 7 shows the content of each component.

Raw material Formulation Example 1 Content (w / w%) WE-1 of Example 1 50.0 Butylene Glycol 7.0 glycerin 5.0 Polyoxyethylene (60) hardening castor oil 0.2 ethanol 5.0 Betaine 2.0 Citric acid 0.02 Sodium citrate 0.06 antiseptic a very small amount Spices a very small amount Purified water Remaining amount

Formulation example  2. Lotion ( emulsion )

Pear extract, butylene glycol, glycerin, carboxyvinyl polymer, arginine, preservative and purified water obtained in the above example were heated with stirring at 70 to 75 ℃. Squalane, butylene glycol dicaprylate / dicaprate, sorbitan stearate, polysorbate 60, glyceryl stearate and stearyl glycerate mixture, which were heated by stirring at 75 to 80 ° C. were added thereto, followed by emulsification. When the mixture was cooled to about 45 ° C. while stirring, the fragrance was added thereto, stirred, cooled to 30 ° C., and aged to prepare a sari extract-containing lotion. Table 8 shows the content of each component.

Raw material Formulation Example 2 Content (w / w%) EE-2 of Example 4 40.0 Butylene glycol 8.0 glycerin 5.0 Squalane 10.0 Butylene Glycol Dicaprylate / Dicaprate 5.0 Sorbitan stearate 1.5 Polysorbate 60 1.0 Glyceryl Stearate 0.5 Stearyl glycyrrhetinate 0.2 Carboxy Vinyl Polymer 0.1 Arginine 0.1 antiseptic a very small amount Spices a very small amount Purified water Remaining amount

Formulation example  3. Essence

Cytocyterol, polyglyceryl 2-oleate, ceramide, ceteareth-4 and cholesterol are stirred and mixed, followed by emulsification with the addition of Pear extract, dicetyl phosphate, concentrated glycerin, and purified water mixed solution, followed by stirring. When cooled to ℃, the fragrance was added and stirred, and cooled to 30 ℃ to mature. To this, carboxyvinyl polymer, xanthan gum, and preservatives were added to stabilize, followed by aging to prepare sari extract containing essence. Table 9 shows the content of each component.

Raw material Formulation Example 3 Content (w / w%) HWE-1 of Example 5 10.0 Cytostolol 1.70 Polyglyceryl 2-oleate 1.50 Ceramide 0.7 Ceteares-4 1.2 cholesterol 1.5 Dicetylphosphate 0.4 Concentrated glycerin 5.0 Carboxy Vinyl Polymer 0.2 Xanthan Gum 0.2 antiseptic a very small amount Spices a very small amount Purified water Remaining amount

Formulation example  4. Cleanser (Cleansing Foam)

Sodium N-acyl glutamate, glycerin, PEG-400, and propylene glycol were added to the purified water, followed by mixing. Small extracts were added in small portions, and EDTA-4Na was added thereto, followed by heating and stirring at 80 ° C. while stirring. To this, POE (15) oleyl alcohol ether, laurin derivative and methyl paraben mixed solution heated at 80 ° C. were added and stirred, followed by the addition of fragrance, followed by gradual cooling to prepare a sari extract-containing cleanser. Table 10 shows the content of each component.

Raw material Formulation Example 4 Content (w / w%) WE-2 of Example 2 20.0 N-acyl glutamate 20.0 glycerin 10.0 PEG-400 15.0 Propylene glycol 10.0 POE (15) oleyl alcohol ether 3.0 Lauric derivatives 2.0 Methyl paraben 0.2 EDTA-4Na 0.03 Spices 0.2 Purified water Remaining amount

Formulation example  5. Shampoo

Glycerine and EDTA-4Na were added to purified water, and the resulting mixture was heated and dissolved at 80 DEG C, followed by stirring by adding TEA lauryl sulfate, sodium lauryl ether sulfate, lauryl amidopropyl betaine and lauryl acid diethanol amide. Citric acid was added thereto and neutralized at 50 ° C., and then P. extract and Zinc pyrithione were added and stirred at 45 ° C. to prepare a sari extract-containing shampoo. Table 11 shows the content of each component.

Raw material Formulation Example 5 Content (w / w%) EE-1 of Example 3 20.0 TEA Lauryl Sulfate 20.0 Sodium Lauryl Ether Sulfate 30.0 Lauryl Amidopropyl Betaine 2.0 Lauryl acid diethanol amide 3.0 glycerin 3.0 EDTA-2Na 0.05 Methyl paraben 0.2 Citric acid 0.03 Zinc pyrithione 0.02 Spices 0.2 Purified water Remaining amount

      Pure rain berry extract of the present invention has a high polyphenol content, excellent electron donating ability, SOD-like activity, nitrite scavenging activity, xanthine oxidase inhibitory activity and tyrosinase inhibitory activity, it is useful for skin aging and whitening cosmetics Can be.

Claims (4)

Hibiscus Vitex rotundifolia L.) Skin anti-aging and whitening cosmetic composition containing the extract as an active ingredient. According to claim 1, wherein the extract is a cosmetic composition, characterized in that the extract is extracted from the seed or stem of hibiscus. The extract of claim 1, wherein the extract is an extract available in water including purified water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof, propylene glycol, ethyl acetate, butylene glycol, ether, or chloroform. Cosmetic composition. The cosmetic composition according to claim 1, wherein the composition is in the form of a flexible lotion (skin), cream, emulsion (lotion), essence, nourishing water, cleaning liquid, pack, cosmetic liquid, spray and foundation.

Images