KR20080083596A - Mask pack for face beauty containing water soluble propolis with excellent effects on prevention of aging of the skin and treatment of dermatitis such as atopy and acne - Google Patents

Abstract

A mask pack for face beauty comprising a water soluble propolis and co-enzyme Q10 is provided to show skin whitening and beauty effect, improve children atopic dermatitis and acne, and alleviate adult face wrinkles. A mask pack for face beauty comprises 7.0 wt.% of a water-soluble propolis, 0.7 wt.% of co-enzyme Q10, 7.0 wt.% of corn starch, 3.0 wt.% of yolk powder, 7.0 wt.% of loess powder, 3.0 wt.% of apricot stone powder, 0.7 wt.% of cucumber juice filtrate powder, 0.7 wt.% of aloe juice powder, 17 wt.% of glycerin, 3.0 wt.% of alginic acid, 0.7 wt.% of perfume, and the remaining amount of purified water.

Description

Mask pack for face beauty containing water soluble propolis with excellent effects on prevention of aging of the skin and treatment of dermatitis such as atopy and acne}

The present invention relates to a facial cosmetic mask pack, and more particularly, to a water-soluble propolis cosmetic mask pack that is excellent in preventing skin aging and treating dermatitis such as atopy and acne.

Due to the frequent use of soap, the skin of the human body, especially the face of the person, removes excessive sebum from the skin and softens the stratum corneum to dry the skin, resulting in rough skin and especially sensitive skin. Causes atopic dermatitis caused by inflammation, acne, and epidermal cell formation inhibition.

As known so far for such facial skin beauty, there are beauty soaps, beauty gels and beauty packs.

On the other hand, as a beauty pack product, herbal ingredients such as Cheongung, Angelica, Licorice, Ginseng, Peppermint, Yulmu, Aloe, Astragalus, Rosin, Acorn, etc. are mixed in the same amount to extract water-soluble components and volatile liquids such as alcohol, glycerin and distilled water. The method of manufacturing a facial cosmetic pack, which is impregnated with a nonwoven fabric or gauze, and then packaged in vinyl or silver foil, has been disclosed in Korean Patent No. 1996-28903.

Gel-type skin care care moisturizer is disclosed in Korean Patent Publication No. 1991-18008, which is a water-soluble thickener mixed with an oily active substance and a hydrophilic active substance, and may have a moisturizing effect after washing the face. Due to the use of artificial preservatives or chemicals such as thickeners and preservatives, skin irritation was frequently caused.

In order to circumvent such chemicals, a cosmetic soap composition using natural mung bean extract and bamboo salt is disclosed in Korean Patent Publication No. 1995-8672, which reduces skin irritation but has a slight improvement in cosmetic effects.

In addition, although a massage gel solvent composition using propolis, which is known to prevent anti-inflammatory action and allergies, is described in Korean Patent Publication No. 2000-56433, propolis is an alcohol or solvent in the process of refining it. When coming out as a product, it is difficult to manufacture a nonwoven fabric absorbing material based on propolis, which is easy to be absorbed into the skin as the product comes out as a particle or propolis solution. In addition, in the case of water-insoluble propolis can not be mixed evenly with other ingredients can be added a small amount of propolis, but the anti-inflammatory and anti-allergic effect of the propolis due to this is difficult to expect.

Accordingly, the present inventors have made a lot of efforts to manufacture a mask pack using propolis, as a result of the present invention for producing a cosmetic mask pack having a cosmetic function, atopic dermatitis improvement function, adult acne treatment effect and adult facial wrinkle relief effect It came to invention.

Therefore, the purpose of the present invention is a novel facial cosmetic function of the novel form has not been proposed so far, has atopic dermatitis improvement in children, cosmetic mask pack using a water-soluble propolis excellent in adult acne treatment and adult facial wrinkle relief effect It is to provide a composition and its products.

The above object of the present invention was conceived in view of the disadvantages of the conventional and known techniques as described above to verify the antimicrobial activity of the water-soluble propolis used in the present invention, the water-soluble propolis as an active ingredient, and the thickener, nutrient Using a material such as a whitening agent, a moisturizer, a softening agent to obtain a desirable cosmetic pack composition and confirming the preferred manufacturing method was achieved by conducting atopic skin treatment efficacy, acne improvement efficacy and other wrinkle removal improvement experiments through clinical trials.

The natural product used in the present invention may be cereals, fruit vegetables, leafy vegetables, fruits, animal materials, secondary soil water in addition to the known herbal medicine.

The most preferable cereals are rice, corn, green beans, potatoes, sweet potato starch, fruit vegetables such as cucumber and eggplant, and leaf vegetables such as aloe and spinach. Also preferred are fruits such as apricot seed, peach seed grounds, animal materials such as egg and duck egg yolk, and secondary soil minerals such as ocher and zucchini.

As the additive according to the present invention, as the softening agent for improving the fluidity of the base (base) and the composition, glycerin and olive oil are preferable, and alginate and hyaluronic acid are preferable as the skin moisturizing agent.

Children's atopic dermatitis derived from environmental hormones and cosmetics remaining on the face according to the present invention prevents adult atopic dermatitis and decomposes various types of waste products such as sebum, keratin, sweat, etc. As a natural antibiotic that does not trouble the skin for effective inhibitory action of acne bacteria, propolis derived from honeycomb is most preferred.

Moreover, the water-soluble propolis has a feature that does not require the use of chemicals such as artificial preservatives because it has an excellent effect of preventing the discoloration or discoloration of the mask pack according to the present invention.

Most preferably, the base of the composition of the present invention is kneaded with starch derived from the grains and mixed with egg yolk powder of animal material or mud powder of secondary soil minerals in a ratio of 3: 7. Most preferably used.

The material of the mask pack according to the present invention is preferably provided with the following conditions.

The propolis may be any one produced by a commonly known method derived from honeycomb, but one purified by nanotechnology is most preferred because it can enhance the effect as a natural antibiotic.

Coenzyme Q10 (produced by Nature's Laboratory Co., Ltd., UK) is preferred, but if there is no problem in purity, other products may be used, and it is involved in anti-wrinkle production by preventing skin aging by strong antioxidant activity.

The starch serving as the base material of the present invention is preferably corn starch, but rice starch, mung bean starch, potato starch, sweet potato starch may also be used.

These starches perform the action of forming colloids with other materials of the present invention to provide the desired adhesion.

Eggs of chickens or ducks are egg yolk containing IgY, which acts as a natural antibody supplying agent, and thus are more preferable and are useful materials of the present invention only for the purpose of nourishing the skin.

Loess or Jiji soil should not be contaminated domestically and should be contaminated.It activates cell physiology through the supply of far-infrared rays to the skin and is effective as a supplementary effect of the skin whitening effect by decomposing and discharging harmful components in cells based on thermal energy. Do.

Cucumber juice powder and aloe juice filter powder were selected to be involved in maintaining the moisturizing power of the skin, maintaining elasticity, regenerating skin cells, removing wrinkles, and whitening through acting as a source of vitamin A and water supply. .

Apricot seed powder has anti-allergic effect on the skin, and the remaining additives, glycerin and alginic acid extracted from seaweed, are mixed with purified water added in the remaining amount together with the fragrance to maintain skin scent by skin moisturizing, skin elasticity, and propolis addition. Play a role.

Preferred blending composition of the material in the present invention is the same as in Example 1, most preferably 7.0% by weight of propolis, 0.7% by weight of coenzyme Q10, 7.0% by weight of corn starch, 3.0% by weight of yolk powder, 7.0% by weight of ocher powder, Apricot seed powder 3.0%, cucumber juice filtrate powder 0.7% by weight, aloe juice powder 0.7% by weight, glycerin 17% by weight, alginic acid 3.0% by weight, fragrance 0.7% by weight and the remaining amount of purified water.

At this time, the kneaded material composition was prepared by the method of Example 2, but put into a press to form a mask of a face-shaped nonwoven fabric and impregnated therein, and then a gauze having the same shape as the mask was attached to the inside of the mask and vacuumed. Packed and stored for use as needed.

The mask pack manufactured according to the present invention is used in close contact with the face, and when using the mask pack dried by a dryer, it is briefly submerged in purified water and then used.

The facial cosmetic mask pack composition of the present invention is characterized in that it is particularly effective in improving atopic skin disease and preventing acne and reducing facial wrinkles in adults.

The present invention relates to a water-soluble propolis cosmetic mask pack that is excellent in preventing skin aging and treating dermatitis such as atopic dermatitis and acne. The mask pack of the present invention is effective in improving children's atopic dermatitis, preventing adult acne, as well as removing adult wrinkles. It is also a very useful invention in the cosmetic industry because it has a very good effect.

The present invention will be described with reference to the preferred embodiments, but any invention made by a person of ordinary skill in the art can infringe the right of the present invention by any change in materials or by the circumvention of the manufacturing method. Of course it belongs.

Example  1: Determination of antimicrobial activity of water soluble propolis

Preparation of reagents and materials

Propolis extracted from the honeycomb was purified by nanotechnology to prepare a high purity water-soluble propolis, and streptomycin (streptomycin) for the measurement of antimicrobial activity was purchased from Sigma-Aldrich (Sigma-Aldrich).

The strain used in this experiment was Escherichia coli KCCM 11234) was used by the Korean spawn association. The bacterial growth medium was used for 24 hours shaking culture in Luria-Butani medium (LB, pH7.0, 37 ℃). The composition of the medium used for the growth of the strain is shown in Table 1.

Composition of LB Medium Furtherance content Trypton 10 g Yeast extract 5 g Sodium chloride 10 g Agar 15 g Distilled water 1L

Disk Spread Test ( Disc diffusion test )

After incubating the bacteria at 36 ° C. for 1-2 days, the bacteria were inoculated and suspended to contain 1 × 10 ⁶ cells per ml of the medium. The cells were plated on agar medium, and the medium was sufficiently dried, and then the paper disc was seated by aseptic manipulation (autoclave 121 ° C., 15 minutes). After 24 hours of incubation at the optimum growth temperature of the growth inhibition was detected by the presence of a clean zone (clean zone). First, a blank test of the strain of E. coli on the fiber obtained by spinning cellulose acetate (cellulose acetate). As a result of the experiment, it was found that the growth-lowering ring was not formed around the cellulose acetate fiber, and thus the cellulose acetate fiber did not affect the growth of the bacteria.

A cellulose acetate solution containing 0.1, 0.5, 1.0, 5.0, 10, 20, 30 wt% propolis was spun. Discharging the spun fiber by collecting the spun fibers from the collector plate and punching the fibers containing propolis into 6 mm diameters of 50 mm in size and settled on 100 µl of E. coli smeared media. Was carried out. Results As shown in FIG. 1, as a result of the disk diffusion method for the cellulose acetate fiber containing propolis for E. coli , it was found that the size of the growth-lowering ring increased as the content of the propolis increased.

Optical density  Test (absorbance test; Optical density test )

E. coli for optical density testing 1 mL of strain culture was inoculated into LB medium, which is a growth medium. After adding each additive to the inoculated broth on a weight basis, incubate at optimum growth conditions (36 ℃, 180rpm) in a shaker and at the same time, incubate UV-2401PC spectrophotometer at intervals of 4 hours from the inoculation time. measured at 640 nm with a spectrophotometer (SHIMADZU. Japan). In addition, the broth containing the extract was used as a blank to prevent affecting the results of the optical density test by the turbidity of the extract.

To a sufficiently stirred cellulose acetate solution (12.5 wt%), propolis 0.1, 0.5, 1.0, 5.0, 11, 22 wt% was added to the sufficient agitation, followed by spinning to prepare a propolis-containing cellulose acetate fiber. . 0.5 g of propolis-containing cellulose fibers were precisely weighed and incubated in an optimum growth condition in a shaker, and measured at 640 nm with a spectrophotometer every 4 hours from the inoculation time. Optical density measurement results of the propolis-containing cellulose acetate fibers are shown in FIG. 2. As can be seen in Figure 2, the increase in the concentration of propolis was found to be lower absorbance. This confirmed that the propolis inhibited the growth of bacteria.

Bacterial growth inhibition rate

Bacteria reduction was calculated by the following equation using the results of the optical density analysis method. Bacterial growth inhibition rate represents the proliferation removal rate of the bacteria of the test sample containing the additive to the control sample without the additive.

                    Control Absorbance-Sample Absorbance

Germ growth removal rate (%) = -------------------------- × 100

                       Control absorbance

The calculation results are shown graphically in FIG. 3. Electrospun fiber containing 2 wt% of natural extract of propolis has a high germ growth inhibition rate of more than 90% during the initial 32 hours, and steadily decreases after 32 hours and reaches a constant rate of 72% after 44 hours. Proliferation inhibition rate was shown. From the above results, it was confirmed that the water-soluble propolis effectively inhibited the growth of bacteria.

Colony Counting  Test( colony counting test )

Antimicrobial activity of fiber against E. coli was measured by colony counting. Sufficient growth of the E. coli strain was diluted 10 ⁴ fold by serial dilution using a dilute phosphate buffer. 1 mL of the diluted strain was added to 100 mL of phosphate buffer solution and diluted 10 ⁶ fold. 0.5 g of the fiber prepared by adding each additive to 100 ml of the solution diluted by 10 to ⁶ times was added, and colony counting was performed at intervals of 4 hr, 8 hr, 16 hr, and 24 hr to confirm the number of cells.

The measurement results are shown in FIG. 4. As a result of colony counting, no cells were found in the cellulose acetate fibers containing 0.5 wt% streptomycin. In the case of cellulose acetate fibers containing 2wt% propolis, 98.4% of the cells were killed after 4 hours and 100% after 8 hours. From the results it was confirmed that the propolis has an antibacterial action.

Example  2: using propolis Mask pack  Produce

A mask pack for testing the present invention atopic skin improvement effect, acne improvement effect and wrinkle improvement effect was prepared as follows according to the composition ratio of Table 2.

Composition ratio of mask pack using water soluble propolis division Composition example 1 Composition example 2 Composition example 3 Composition example 4 Composition example 5 Composition example 6 Propolis Extract 5.0 6.0 7.0 8.0 9.0 10.0 Coenzyme Q10 0.5 0.6 0.7 0.8 0.9 1.0 Corn starch 5.0 2.0 7.0 8.0 9.0 10.0 Egg yolk powder 1.0 6.0 3.0 4.0 5.0 6.0 Ocher powder 5.0 2.0 7.0 8.0 9.0 10.0 Apricot Seed Powder 1.0 0.6 3.0 4.0 5.0 6.0 Cucumber juice filtrate powder 0.5 0.6 0.7 0.8 0.9 1.0 Aloe Juice Powder 0.5 16 0.7 0.8 0.9 1.0 glycerin 15 2.0 17 18 19 20 Alginic acid 1.0 0.6 3.0 4.0 5.0 6.0 Spices 0.5 0.6 0.7 0.8 0.9 1.0 Remaining amount Purified water Purified water Purified water Purified water Purified water Purified water

Propolis extracted from the honeycomb was purified by nanotechnology to make a high-purity water-soluble propolis, mixed with a base component (moisturizer) and a softener, dissolved in purified water at 60 ℃ and then added coenzyme Q10 and 30 ℃ The composition obtained after lowering to a temperature to dissolve was impregnated into a nonwoven fabric pack to form a mask pack product of the present invention.

In the present invention, when the mixing temperature of the composition is 60 ° C. or less, deterioration of enzymes and antibiotics is a concern.

In particular, it was preferred to lower the temperature below 30 ° C. to obtain the final composition for the activity of coenzyme Q10.

Among the mask packs prepared according to the method of the present invention, the most preferable material composition (Best Mode) in moisturizing effect, flexibility, and the like was shown in Composition Examples 3 and 4.

Hereinafter, the clinical trials used the pack prepared according to the composition example 3.

< Experimental Example  1> Clinical trial for improving atopic dermatitis

Twenty volunteers among children patients diagnosed with atopic dermatitis at S university hospital dermatology using a nonwoven fabric pack containing the propolis of composition example 3 prepared according to Example 2 according to the composition ratio of Table 2 Ten patients were enrolled in the clinical trial.

Clinical changes were observed one week after the parent's parent adhered to the affected child's affected area for 30 minutes once a day for 30 minutes once daily with a nonwoven pack of propolis and coenzyme Q10. Subjective improvement is assessed to parents by the change of overall skin condition through questionnaire, and objective improvement is assessed before and after the test by scoring and summing itching, severe scratching symptoms, blood pressure and pustules at scores of 0, 1, 2, and 3, respectively. It was.

As a result, the subjective improvement was excellent in all parents of 20 children. The objective improvement was good in all four types, but the average improvement in each symptom was 46%.

Objective improvement of the improvement of atopic dermatitis in children of nonwoven pack impregnated with propolis and coenzyme Q10 division Evaluation site First day total score Total score 7 days after experiment Improvement in improvement (%) Man 1 Bridge 5 2 60 Man 2 Forearm 7 5 30 Man 3 Groin 6 4 33 Man 4 Earlobe 4 2 50 Man 5 Bridge 9 5 44 Man 6 Groin 10 7 30 Man 7 Earlobe 11 7 36 Man 8 Earlobe 7 4 43 Man 9 Forearm 5 2 60 Man 10 Bridge 8 5 38 Girls 11 Forearm 6 3 50 Girls 12 Bridge 7 3 57 Girls 13 Bridge 11 6 45 Girls 14 Earlobe 10 7 30 Girls 15 Groin 6 2 67 Girls 16 Groin 9 5 44 Girls 17 Forearm 7 3 57 Girls 18 Forearm 8 3 63 Girls 19 Face 9 4 56 Girls 20 Earlobe 6 3 50 Sum 151 82 943 Average 7.6 4.1 46

[Note] 1. Improved improvement = (sum total score before experiment-sum score after experiment) / rounding off the total score before experiment.

< Experimental Example  2> Clinical trial of acne improvement effect

Clinical trials were carried out on 10 volunteers (5 men and women) among acne patients who received the mask packs prepared using the composition of the best mode used in Experimental Example 1 at S University Hospital Dermatology.

The facial cosmetic mask pack impregnated with propolis and coenzyme Q10 was worn once a day for 1 week after washing for 10 minutes or more and allowed to lie on the bed for 30 minutes, and then clinical changes were observed.

Observation results were divided into erythema, comeedone, papule, and pustule, and scored 0, 1, 2, and 3 according to the experimental example evaluation method. The improvement in objective improvement was evaluated by comparing the states.

The results showed that the objective improvement was 58.5%, erythema 74.2%, comedone 30.3%, papule 57.2% pustule 72.2%, respectively.

Therefore, the use of the mask pack before bedtime according to the composition example 3 of the present invention was found to be excellent in the initial acne prevention effect.

Acne Improvement division Agility (%) erythema 74.2 comedone 30.3 papule 57.2 pustule 72.2 Average 58.5

< Experimental Example  3> Clinical Effect of Wrinkle Removal

Clinical trial of wrinkle removal effect for 20 volunteers in their 30s to 40s entering and leaving the Gangnam H skin care shop using the mask pack of the present invention manufactured by the manufacturing method of Example 2 according to the composition example 3 of Table 2 above Was carried out.

The study was conducted at the shop at 11 am to 12 am every day for three days, followed by a clinical trial.

Wrinkle removal efficacy test was judged that the subjective satisfaction of the applicants rather than the objective evaluation, rather it can represent the objectivity of the efficacy of the present invention was carried out subjective evaluation.

Furthermore, in the dermatology of hospitals and clinics, wrinkles were not skin diseases but rather skin aging or trouble, and the subjective judgments of the applicants were scored and summed.

The test results are shown in Table 5 below.

The points were scored as (A) 0 points, (B) Normal 1 point, (C) Improvement 2 points, (D) Significant improvement 3 points.

Wrinkle removal efficacy clinical trial results (unit: persons) Total number of applicants (persons) (A) (B) (C) (D) Sum Average 20 0 2 7 11 20 Total score 0 2 14 33 49 2.45 % Improvement 10 35 55 100

There were no applicants who reported no wrinkle removal effect (0 patients), and 11 applicants answered that they were significantly improved despite being evaluated after 3 days of 3 days of regular clinical trials. The score was 2.45, which was greatly improved (D).

1 is a photograph showing the antimicrobial effect of E. coli by the disk diffusion method of various concentrations of propolis fibers. (a) is 0.1wt% of propolis fibers, (b) is 1.0wt%, (c) is 10wt%, and (d) is the result of the concentration of 30wt%.

Figure 2 is a graph measuring the absorbance of the culture time of E. coli by the addition of propolis-cellulose fiber. ● indicates the growth curve of E. coli , ○ is 0.01wt% propolis, ▼ is 0.05wt%, ▽ is 0.1wt%, ■ is 0.5wt%, □ is 1wt%, ◆ is 2wt% concentration. Meaning, wt% represents dry weight / broth.

Figure 3 is a graph showing the bacterial growth inhibition rate according to the culture time of E. coli by the addition of propolis-cellulose fiber.

Claims (3)

Facial mask pack containing water-soluble propolis and coenzyme Q10 as an active ingredient. According to claim 1, wherein the facial mask pack is water-soluble propolis 7.0% by weight, coenzyme Q10 0.7% by weight, corn starch 7.0% by weight, egg yolk powder 3.0% by weight, ocher powder 7.0% by weight, apricot seed powder 3.0%, Cucumber juice filtrate powder 0.7% by weight, aloe juice powder 0.7% by weight, glycerin 17% by weight, alginic acid 3.0% by weight, fragrance 0.7% by weight and the residual amount of purified water mask pack. After dissolving the composition containing the water-soluble propolis according to claim 1 or 2 as an active ingredient in purified water at 60 ° C. and then adding coenzyme Q10 and lowering it to a temperature of 30 ° C., the resulting composition is impregnated into a nonwoven fabric pack. Method for producing a facial cosmetic mask pack, characterized in that.

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