KR20070093992A - 고품질 핵산의 무세포 생합성 및 그것의 사용 - Google Patents
고품질 핵산의 무세포 생합성 및 그것의 사용 Download PDFInfo
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- KR20070093992A KR20070093992A KR1020077015676A KR20077015676A KR20070093992A KR 20070093992 A KR20070093992 A KR 20070093992A KR 1020077015676 A KR1020077015676 A KR 1020077015676A KR 20077015676 A KR20077015676 A KR 20077015676A KR 20070093992 A KR20070093992 A KR 20070093992A
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- A61P31/14—Antivirals for RNA viruses
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- A61P31/12—Antivirals
- A61P31/20—Antivirals for DNA viruses
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/20—Antivirals for DNA viruses
- A61P31/22—Antivirals for DNA viruses for herpes viruses
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/10—Nucleotidyl transfering
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| CA (1) | CA2590933A1 (enExample) |
| IL (1) | IL183778A0 (enExample) |
| WO (1) | WO2006063355A2 (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20200036925A (ko) * | 2017-08-11 | 2020-04-07 | 제너럴 일렉트릭 캄파니 | 이중-가닥 콘카테머 dna를 사용하는 무세포 단백질 발현 |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007018744A2 (en) * | 2005-08-03 | 2007-02-15 | Cytogenix, Inc. | Cell-free biosynthesis of nucleic acid |
| GB0614825D0 (en) * | 2006-07-26 | 2006-09-06 | Medical Res Council | Method for amplification of ligation reactions |
| AU2008307617B2 (en) * | 2007-09-28 | 2013-05-23 | Pacific Biosciences Of California, Inc. | Error-free amplification of DNA for clonal sequencing |
| FR2924440B1 (fr) * | 2007-12-04 | 2015-01-09 | Pf Medicament | Nouveau procede de generation et de criblage d'une banque d'anticorps |
| US9125845B2 (en) | 2008-07-09 | 2015-09-08 | General Electric Company | DNA vaccines, uses for unprocessed rolling circle amplification product and methods for making the same |
| US20100008939A1 (en) * | 2008-07-09 | 2010-01-14 | General Electric Company | Unprocessed rolling circle amplification product |
| US8921072B2 (en) | 2008-09-02 | 2014-12-30 | General Electric Compnay | Methods to generate DNA mini-circles |
| GB0901593D0 (en) | 2009-01-30 | 2009-03-11 | Touchlight Genetics Ltd | Production of closed linear DNA |
| JP2013126953A (ja) * | 2010-03-31 | 2013-06-27 | Terumo Corp | リポソーム製剤の製造方法 |
| GB201013153D0 (en) * | 2010-08-04 | 2010-09-22 | Touchlight Genetics Ltd | Primer for production of closed linear DNA |
| US10077459B2 (en) * | 2016-05-04 | 2018-09-18 | General Electric Company | Cell-free protein expression using rolling circle amplification product |
| US10350307B2 (en) | 2017-09-18 | 2019-07-16 | General Electric Company | In vivo RNA or protein expression using double-stranded concatemeric DNA including phosphorothioated nucleotides |
| GB201901583D0 (en) * | 2019-02-05 | 2019-03-27 | Ducani Cosimo | Method and products for producing funtionaslised single stranded oligonucleotides |
| JP2022549138A (ja) | 2019-09-18 | 2022-11-24 | インターガラクティック セラピューティクス インコーポレイテッド | 合成ｄｎａベクターおよびその使用法 |
| CN115175998A (zh) * | 2019-10-18 | 2022-10-11 | Encodia 公司 | 分析用大分子的自动化处理及相关设备 |
| GB202002547D0 (en) * | 2020-02-24 | 2020-04-08 | Moligo Tech Ab | Method and products for producing single stranded DNA polynucleotides |
| EP4083227A1 (en) * | 2021-04-29 | 2022-11-02 | 4basebio, S.L.U. | Linear dna with enhanced resistance against exonucleases |
| US20240409975A1 (en) * | 2021-09-27 | 2024-12-12 | Intergalactic Therapeutics, Inc. | Synthetic production of circular dna vectors |
| EP4419677A4 (en) | 2021-10-18 | 2025-09-24 | Flagship Pioneering Innovations Vii Llc | DNA COMPOSITIONS AND RELATED METHODS |
| EP4310182A1 (en) * | 2022-07-19 | 2024-01-24 | 4basebio UK Ltd | Protected dna and methods for the production thereof |
| WO2025175112A1 (en) * | 2024-02-16 | 2025-08-21 | University Of Maryland, Baltimore County | Method for generating circular dna using heteroduplex thermostable ligation assembly of precursors produced by rolling circle amplification |
Family Cites Families (60)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5750338A (en) * | 1986-10-23 | 1998-05-12 | Amoco Corporation | Target and background capture methods with amplification for affinity assays |
| US4994372A (en) * | 1987-01-14 | 1991-02-19 | President And Fellows Of Harvard College | DNA sequencing |
| CA1340807C (en) * | 1988-02-24 | 1999-11-02 | Lawrence T. Malek | Nucleic acid amplification process |
| US5198543A (en) * | 1989-03-24 | 1993-03-30 | Consejo Superior Investigaciones Cientificas | PHI29 DNA polymerase |
| US5001050A (en) * | 1989-03-24 | 1991-03-19 | Consejo Superior Investigaciones Cientificas | PHφ29 DNA polymerase |
| US5043272A (en) * | 1989-04-27 | 1991-08-27 | Life Technologies, Incorporated | Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers |
| US5455166A (en) * | 1991-01-31 | 1995-10-03 | Becton, Dickinson And Company | Strand displacement amplification |
| DK51092D0 (da) * | 1991-05-24 | 1992-04-15 | Ole Buchardt | Oligonucleotid-analoge betegnet pna, monomere synthoner og fremgangsmaade til fremstilling deraf samt anvendelser deraf |
| US5834202A (en) * | 1992-08-04 | 1998-11-10 | Replicon, Inc. | Methods for the isothermal amplification of nucleic acid molecules |
| US6261808B1 (en) * | 1992-08-04 | 2001-07-17 | Replicon, Inc. | Amplification of nucleic acid molecules via circular replicons |
| US6410277B1 (en) * | 1993-02-19 | 2002-06-25 | Takara Shuzo Co., Ltd. | DNA polymersases with enhanced length of primer extension |
| US5432065A (en) * | 1993-03-30 | 1995-07-11 | United States Biochemical Corporation | Cycle sequencing with non-thermostable DNA polymerases |
| US6096880A (en) * | 1993-04-15 | 2000-08-01 | University Of Rochester | Circular DNA vectors for synthesis of RNA and DNA |
| US5714320A (en) * | 1993-04-15 | 1998-02-03 | University Of Rochester | Rolling circle synthesis of oligonucleotides and amplification of select randomized circular oligonucleotides |
| CA2135579A1 (en) * | 1993-11-12 | 1995-05-13 | Gerald James Mcnabb | Solid fertilizer with a modifier bound to its surfaces |
| US5561064A (en) * | 1994-02-01 | 1996-10-01 | Vical Incorporated | Production of pharmaceutical-grade plasmid DNA |
| WO1995028175A1 (en) * | 1994-04-19 | 1995-10-26 | Thomas Jefferson University | Viral ribonucleocapsid as an immunological enhancer |
| US6054299A (en) * | 1994-04-29 | 2000-04-25 | Conrad; Charles A. | Stem-loop cloning vector and method |
| US5614365A (en) * | 1994-10-17 | 1997-03-25 | President & Fellow Of Harvard College | DNA polymerase having modified nucleotide binding site for DNA sequencing |
| US5602011A (en) * | 1995-01-18 | 1997-02-11 | Pharmacia Biotech Inc. | Purified Thermococcus barossii DNA polymerase |
| WO1996038568A1 (en) * | 1995-05-31 | 1996-12-05 | Amersham Life Science, Inc. | Thermostable dna polymerases |
| JP3974941B2 (ja) * | 1995-11-21 | 2007-09-12 | イェール ユニバーシティ | 単分子セグメントの増幅および検出 |
| US5854033A (en) * | 1995-11-21 | 1998-12-29 | Yale University | Rolling circle replication reporter systems |
| EP1300466B1 (en) * | 1995-12-05 | 2006-04-26 | Jorn Erland Koch | A cascade nucleic acid amplification reaction |
| DE69613856T2 (de) * | 1995-12-15 | 2002-04-04 | Amersham Pharmacia Biotech Inc., Piscataway | Thermostabile dna polymerase aus thermoanaerobacter thermohydrosulfuricus und davon abgeleitete mutierte enzyme mit entfernter exonukleaseaktivität |
| US5827716A (en) * | 1996-07-30 | 1998-10-27 | Amersham Life Science, Inc. | Modified Pol-II type DNA polymerases |
| US6011148A (en) * | 1996-08-01 | 2000-01-04 | Megabios Corporation | Methods for purifying nucleic acids |
| US6042832A (en) * | 1996-08-28 | 2000-03-28 | Thomas Jefferson University | Polypeptides fused with alfalfa mosaic virus or ilarvirus capsid proteins |
| US6200959B1 (en) * | 1996-12-04 | 2001-03-13 | Powerject Vaccines Inc. | Genetic induction of anti-viral immune response and genetic vaccine for filovirus |
| US5882904A (en) * | 1997-08-04 | 1999-03-16 | Amersham Pharmacia Biotech Inc. | Thermococcus barossii DNA polymerase mutants |
| US6124120A (en) * | 1997-10-08 | 2000-09-26 | Yale University | Multiple displacement amplification |
| US6709844B1 (en) * | 2000-11-16 | 2004-03-23 | Mannkind Corporation | Avoidance of undesirable replication intermediates in plasmid propagation |
| US6787305B1 (en) * | 1998-03-13 | 2004-09-07 | Invitrogen Corporation | Compositions and methods for enhanced synthesis of nucleic acid molecules |
| JP4493844B2 (ja) * | 1998-03-25 | 2010-06-30 | ランデグレン、ウルフ | 錠型（ｐａｄｌｏｃｋ）プローブのローリングサークル複製 |
| US6479267B1 (en) * | 1998-06-17 | 2002-11-12 | Amersham Pharmacia Biotech, Inc. | FY7 polymerase |
| WO2000004193A1 (en) * | 1998-07-20 | 2000-01-27 | Yale University | Method for detecting nucleic acids using target-mediated ligation of bipartite primers |
| WO2000015849A1 (en) * | 1998-09-15 | 2000-03-23 | Yale University | Artificial long terminal repeat vectors |
| US6235502B1 (en) * | 1998-09-18 | 2001-05-22 | Molecular Staging Inc. | Methods for selectively isolating DNA using rolling circle amplification |
| DE69940623D1 (de) * | 1998-11-09 | 2009-04-30 | Eiken Chemical | Prozess zur Synthetisierung von Nukleinsäure |
| US6830884B1 (en) * | 1998-12-15 | 2004-12-14 | Molecular Staging Inc. | Method of amplification |
| JP2000225335A (ja) * | 1999-02-04 | 2000-08-15 | Shimadzu Corp | 自動合成装置 |
| US6300069B1 (en) * | 1999-05-03 | 2001-10-09 | Qiagen Gmbh | Generation and amplification of nucleic acids from ribonucleic acids |
| US6875619B2 (en) * | 1999-11-12 | 2005-04-05 | Motorola, Inc. | Microfluidic devices comprising biochannels |
| EP1232270A1 (en) * | 1999-11-23 | 2002-08-21 | Amersham Biosciences Corp. | IMPROVING DIDEOXYNUCLEOTIDE-TRIPHOSPHATE UTILIZATION BY THE HYPER-THERMOPHILIC DNA POLYMERASE FROM THE ARCHAEON i PYROCOCCUS FURIOSUS /i |
| US6221603B1 (en) * | 2000-02-04 | 2001-04-24 | Molecular Dynamics, Inc. | Rolling circle amplification assay for nucleic acid analysis |
| AU2001240109A1 (en) * | 2000-03-07 | 2001-09-17 | U.S. Army Medical Research Institute Of Infectious Diseases | Dna vaccines against poxviruses |
| US6291187B1 (en) * | 2000-05-12 | 2001-09-18 | Molecular Staging, Inc. | Poly-primed amplification of nucleic acid sequences |
| US6323009B1 (en) * | 2000-06-28 | 2001-11-27 | Molecular Staging, Inc. | Multiply-primed amplification of nucleic acid sequences |
| WO2002066611A2 (en) * | 2001-02-16 | 2002-08-29 | The Board Of Trustees Of The Leland Stanford Junior University | Minimal plasmid vectors that provide for persistent and high level gene expression and methods for using the same |
| GB2378245A (en) * | 2001-08-03 | 2003-02-05 | Mats Nilsson | Nucleic acid amplification method |
| US6617137B2 (en) * | 2001-10-15 | 2003-09-09 | Molecular Staging Inc. | Method of amplifying whole genomes without subjecting the genome to denaturing conditions |
| US7309575B2 (en) * | 2002-01-16 | 2007-12-18 | The University Of North Carolina At Chapel Hill | Protein purification and detection methods |
| US20030180781A1 (en) * | 2002-03-25 | 2003-09-25 | The Regents Of The University Of California | Constructing very long DNA sequences from synthetic DNA molecules |
| US6815167B2 (en) * | 2002-04-25 | 2004-11-09 | Geneohm Sciences | Amplification of DNA to produce single-stranded product of defined sequence and length |
| WO2004020605A2 (en) * | 2002-08-29 | 2004-03-11 | The Board Of Trustees Of The Leland Stanford Junior University | Circular nucleic acid vectors, and methods for making and using the same |
| CA2513535C (en) * | 2003-01-29 | 2012-06-12 | 454 Corporation | Bead emulsion nucleic acid amplification |
| JP2006042601A (ja) * | 2003-04-25 | 2006-02-16 | Yaeta Endo | ハイスループット合成システム |
| WO2004097003A2 (en) * | 2003-04-29 | 2004-11-11 | Amersham Biosciences Corp | Multiply-primed amplification of nucleic acid sequences |
| US20040223885A1 (en) * | 2003-05-06 | 2004-11-11 | Keen Randy E. | Apparatus for the automated synthesis of polynucleotides |
| GB0315160D0 (en) * | 2003-06-28 | 2003-08-06 | Royal Holloway University Of L | In vitro amplification of DNA |
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2005
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- 2005-12-12 EP EP05853854A patent/EP1819828A2/en not_active Withdrawn
- 2005-12-12 US US11/792,800 patent/US20080305142A1/en not_active Abandoned
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2007
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20200036925A (ko) * | 2017-08-11 | 2020-04-07 | 제너럴 일렉트릭 캄파니 | 이중-가닥 콘카테머 dna를 사용하는 무세포 단백질 발현 |
Also Published As
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| CA2590933A1 (en) | 2006-06-15 |
| WO2006063355A3 (en) | 2006-10-19 |
| IL183778A0 (en) | 2007-09-20 |
| WO2006063355A2 (en) | 2006-06-15 |
| EP1819828A2 (en) | 2007-08-22 |
| JP2008522628A (ja) | 2008-07-03 |
| AU2005314431A1 (en) | 2006-06-15 |
| BRPI0515777A (pt) | 2008-08-05 |
| US20080305142A1 (en) | 2008-12-11 |
| AU2005314431B2 (en) | 2011-02-17 |
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