KR20060084737A - 다중 바이오어세이용 미세유체 칩 및 그 제조방법 - Google Patents
다중 바이오어세이용 미세유체 칩 및 그 제조방법 Download PDFInfo
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- KR20060084737A KR20060084737A KR1020050005533A KR20050005533A KR20060084737A KR 20060084737 A KR20060084737 A KR 20060084737A KR 1020050005533 A KR1020050005533 A KR 1020050005533A KR 20050005533 A KR20050005533 A KR 20050005533A KR 20060084737 A KR20060084737 A KR 20060084737A
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- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00718—Type of compounds synthesised
- B01J2219/0072—Organic compounds
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/12—Specific details about manufacturing devices
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0636—Integrated biosensor, microarrays
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0887—Laminated structure
Abstract
Description
Claims (12)
- 밑면에 복수의 샘플 채널과 프로브 채널을 위한 오목부를 갖는 채널 구조체를 기판위에 접합하여 미세유체 채널(microfluidic channels)을 제작하는 단계, 상기 프로브 채널 내의 샘플 채널과의 교차점에 프로브 고정화 영역을 제작하는 단계, 상기 프로브 고정화 영역의 프로브 채널 앞뒤에 표적 샘플 간의 혼합 방지용 차단벽(blocking wall)을 제작하는 단계를 포함하는 다중 바이오어세이(multiple bioassay)용 미세유체 칩(microfluidic chip)의 제조방법.
- 제 1항에 있어서, 상기 프로브는 DNA, RNA, PNA(Peptide Nucleic Acid), LNA(Locked Nucleic Acid), 펩타이드 및 단백질로 구성된 군에서 선택된 바이오분자인 것을 특징으로 하는 미세유체 칩의 제조방법.
- 제 1항에 있어서, 상기 프로브 고정화 영역은 바이오분자 결합기를 갖는 폴리머의 광 중합(photopolymerization)에 의해 제작되는 것을 특징으로 하는 미세유체 칩의 제조방법.
- 제 3항에 있어서, 상기 폴리머는 글리시딜 메타크릴레이트(GMA) 중합체인 것을 특징으로 하는 미세유체 칩의 제조방법.
- 제 1항에 있어서, 상기 차단벽은 바이오분자 결합기가 없는 폴리머의 광 중합(photopolymerization)에 의해 제작되는 것을 특징으로 하는 미세유체 칩의 제조방법.
- 제 5항에 있어서, 상기 폴리머는 폴리에틸렌글리콜 디아크릴레이트(PEG-DA) 중합체인 것을 특징으로 하는 미세유체 칩의 제조방법.
- 제 1항에 있어서, 상기 채널 구조체는 유리, PDMS 또는 폴리머로 된 단일 층(single layer)인 것을 특징으로 하는 미세유체 칩의 제조방법.
- 제 1항에 있어서, 상기 기판은 실리콘 또는 유리로 된 것을 특징으로 하는 미세유체 칩의 제조방법.
- 제 1항에 있어서, 상기 프로브 고정화 영역은 그 높이가 미세유체 채널의 천장에 못미치게 하는 것을 특징으로 하는 미세유체 칩의 제조방법.
- 제 9항에 있어서, 상기 프로브 고정화 영역의 폴리머 광중합시 채널 구조체에 압력을 가하고 중합이 끝난후에 압력을 제거하는 것을 특징으로 하는 미세유체 칩의 제조방법.
- 제 9항에 있어서, 상기 프로브 고정화 영역의 폴리머 광중합시 UV 투여량 제어(dose control)에 의해 부분 중합(partially polymerization)하는 것을 특징으로 하는 미세유체 칩의 제조방법.
- 기판, 그 위에 접합된 단일층 채널 구조체, 채널 구조체내에 형성된 복수의 샘플 채널과 프로브 채널, 상기 프로브 채널과 샘플 채널의 교차점에 형성된 프로브 고정화 영역, 상기 프로브 고정화 영역의 프로브 채널 앞뒤에 형성된 표적 샘플 간의 혼합 방지용 차단벽(blocking wall)을 포함하는 다중 바이오어세이(multiple bioassay)용 미세유체 칩(microfluidic chip).
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KR1020050005533A KR100682920B1 (ko) | 2005-01-20 | 2005-01-20 | 다중 바이오어세이용 미세유체 칩 및 그 제조방법 |
US11/335,939 US7670772B2 (en) | 2005-01-20 | 2006-01-20 | Microfluidic chip for multiple bioassay and method of manufacturing the same |
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Cited By (2)
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KR101335725B1 (ko) * | 2008-10-02 | 2013-12-04 | 삼성전자주식회사 | 다중분석용 미세유동 구조물 및 이를 포함하는 미세유동 장치 |
US8741974B2 (en) | 2008-01-07 | 2014-06-03 | Samsung Electronics Co., Ltd. | Method of forming filter in fluid flow path in microfluidic device |
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KR100930859B1 (ko) | 2007-08-22 | 2009-12-10 | 한양대학교 산학협력단 | 동시 다중면역 반응 측정을 위한 마이크로 바이오칩과 이의제조방법 및 이를 이용한 면역반응 여부를 검출하는 방법 |
KR100968640B1 (ko) | 2007-10-06 | 2010-07-06 | 재단법인서울대학교산학협력재단 | 단일세포의 신호분석방법 |
WO2009126826A1 (en) | 2008-04-11 | 2009-10-15 | Fluidigm Corporation | Multilevel microfluidic systems and methods |
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GB201106254D0 (en) | 2011-04-13 | 2011-05-25 | Frisen Jonas | Method and product |
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CA2916660C (en) | 2013-06-25 | 2022-05-17 | Prognosys Biosciences, Inc. | Spatially encoded biological assays using a microfluidic device |
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KR20050055336A (ko) * | 2003-12-08 | 2005-06-13 | 한국항공우주연구원 | 삽입홈에 나사가 형성된 삽입식 턴버클 |
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Cited By (2)
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US8741974B2 (en) | 2008-01-07 | 2014-06-03 | Samsung Electronics Co., Ltd. | Method of forming filter in fluid flow path in microfluidic device |
KR101335725B1 (ko) * | 2008-10-02 | 2013-12-04 | 삼성전자주식회사 | 다중분석용 미세유동 구조물 및 이를 포함하는 미세유동 장치 |
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US20060188906A1 (en) | 2006-08-24 |
US7670772B2 (en) | 2010-03-02 |
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