KR20050108746A - Composition comprising an extract of trapa japonica flerov. showing antioxidative effect - Google Patents

Abstract

The present invention relates to a composition containing an antioxidant extract as an active ingredient, the extract of the present invention exhibits excellent efficacy in increasing the antioxidant activity and reduction of free radicals involved in inhibiting aging, It can be usefully used in health functional foods, cosmetics and medicines that are effective in the prevention and treatment of the same diseases.

Description

Composition comprising an extract of Trapa japonica Flerov. showing antioxidative effect}

The present invention relates to a composition containing a dry extract having antioxidant activity as an active ingredient.

The major causes of death in Korea in 2003 were cancer, cerebrovascular disease, cardiovascular disease, and diabetes mellitus (Korea National Statistical Office: The cause of death Statistics 2003. Annual Report of on the Cause of Death Statistics.Korea National Statistical Office, 2004 ). Reactive oxygen species (ROS) may be a major factor causing chronic degenerative diseases such as cardiovascular disease, diabetes mellitus, cancer, degenerative neuropathy and aging, and antioxidants are known to help prevent chronic degenerative diseases. (Bray, TM Nutrition. 16 , pp 578-581, 2000). Since all aerobic organisms inevitably carry harmful free radical species during metabolism, all organisms must have a mechanism to remove these free radical species in order to survive. Active oxygen species can be broadly classified into two types: compounds that form oxygen radical species, such as hydrogen peroxide, and oxygen radical species. As already known, the hydrogen peroxide is catalase (catalase) or the case of peroxidase (peroxidase) oxygen, but the conversion of water, oxygen radical species are removed by the mechanism, O ₂ ^- other oxygen radicals other than the mechanism of removing (superoxide anion) Species removal mechanisms are not yet clear.

Hydroxy radicals, a type of oxygen radical species, are formed by the reaction of H ₂ O ₂ and O ₂ in living organisms and are known to exist in almost all biomolecules (Fridovich et al., Ann. Rev. Biochem ., 44 , pp 147-159, 1975; Tauber et al., Blood , 53, pp 666-676, 1979). Therefore, it is obvious that defense against highly reactive hydroxy radicals is required in order to maintain homeostasis in vivo. In this respect, organic materials such as ascorbic acid and uric acid are known to remove hydroxy radicals by chemical reaction, but this is not fundamentally to remove hydroxy radicals but to convert them into less reactive radicals, as well as oxidizing agents in vivo. It has been reported that it can react with transition metals such as iron, but rather promote the production of active oxygen radical species (Borg, DC and Schaich, KM, Handbook of Free Radicals and Antioxidant in Biomedicine, Vol. I, pp 63-). 80, CRC press, 1989). Physiological processes radicals generated during ^{_{(O 2 -, H 2 O}} 2, OH and O ₂₎ is a free radical reaction in which a very strong reactivity caused by these represents the destruction effect of major macromolecules, including lipids (Davies , KJA and Goldberg, AL, J. Biol. Chem., 262 , p8220-8226, 1987). Their production is increased by a number of factors that cause abnormal metabolic processes, photosensitization, drug metabolism, and other cellular metabolism, and therefore, living organisms are always exposed to the harmful effects of free radical reactions caused by them. (Morehouse, LA and Thomas, CE, J. Free Radicals Biol. Med., 1, p8, 1985). In particular, if cells continue to accumulate harmful effects due to their harmful radicals as they age, they cause aging of the cells as well as diseases related to various adult diseases such as carcinogenesis, arteriosclerosis, heart disease, and skin aging. It has been suggested as a cause of human disease occurrence and aging (Harman, D., Free Radicals in Biology , Academic Press, New York, pp 255-275, 1982). Therefore, by inhibiting free radical reactions in vivo by reactive oxygen metabolites or removing harmful free radicals accumulated in the human body, candidates are expected to be useful for the treatment of diseases such as lipid metabolism, immune diseases and cancer. Research has continued.

The dry ( Trapa japonica Flerov .) Of the present invention is aquatic plants distributed throughout Korea, the fruit has been used in the treatment of gastrointestinal diseases, esophageal cancer, uterine cancer, diabetes in folk medicine (민간 元 錐, 中草藥 治療 癌腫.北京: 藥 要 書刊, p103, 1976. Informativeness, metabolic metabolism of primary color, Seoul, Men's Party, p271, 1984), The scientific researches on its efficacy are insufficient. No disclosure or teaching has been made.

Therefore, the present inventors prepared the dried extract using various solvents, and measured the antioxidant enzyme activity. As a result, the present invention was completed by confirming that the antioxidant activity was excellent and thus effective in preventing chronic degenerative diseases.

Accordingly, an object of the present invention is to provide a natural antioxidant composition and health functional food by preparing a dry extract having excellent antioxidant activity.

In order to achieve the above object, the present invention provides an antioxidant composition containing an extract of Trapa japonica Flerov . Having a significant antioxidant effect as an active ingredient.

The extract includes water extracted from C ₁ to C ₄ lower alcohols such as water, ethanol, methanol, or a mixed solvent thereof.

Hereinafter, the present invention will be described in detail.

The dry extract of the present invention can be obtained as follows.

Purity of the present invention, lyophilized and pulverized, the volume of water up to about 2 to 15 times, preferably about 5 to 10 times the weight of the dry sample and C ₁ to C ₄ such as methanol, ethanol, butanol, etc. A lower alcohol polar solvent or a mixed solvent having a mixing ratio of about 1: 0.1 to 1:10, preferably 70 to 100% methanol at room temperature for about 1 hour to 1 day, preferably 6 hours to Using extraction methods such as hot water extraction, cold needle extraction, reflux cooling extraction or ultrasonic extraction for 12 hours, preferably hot water extraction to separate the extract from the residue by filtration under reduced pressure, the dried residue at the beginning of extraction to the obtained residue Polar solvents of water and C ₁ to C ₄ lower alcohols such as methanol, ethanol, butanol and the like, or about 1: 0.1 to 1: 1 volume of water up to about 2 to 10 times, preferably about 3 to 5 times the weight. 10 Mixed solvents having a mixing ratio, preferably 70 to 100% methanol, using an extraction method such as hot water extraction, cold extraction, reflux cooling extraction or ultrasonic extraction for about 1 to 5 hours at room temperature, preferably hot water After extraction, the resultant was filtered under reduced pressure and concentrated to give a dry extract.

In addition, conventional fractionation processes can also be carried out (Harborne JB Phytochemical methods: A guide to modern techniques of plant analysis, 3rd Ed. , Pp 6-7, 1998).

The present invention provides an antioxidant composition containing the dried extract obtained by the above manufacturing process as an active ingredient.

The composition comprises a pharmaceutical composition comprising a pharmaceutically acceptable carrier and an excipient and a cosmetic composition comprising a cosmetically acceptable base.

In addition, the cosmetic composition is a skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisturizing cream, hand cream, foundation, essence, nutrition essence, pack, soap , Formulations of cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser.

As a result of measuring the free radical scavenging ability of the dried extract obtained by the above method, it showed an antioxidant activity similar to L-ascorbic acid as a standard.

In addition, the dried medicinal herbs that have been used for a long time as edible or herbal extracts of the present invention extracted therefrom also have no problems such as toxicity and side effects.

The antioxidant pharmaceutical composition of the present invention comprises the extract in an amount of 0.02 to 50% by weight based on the total weight of the composition.

Pharmaceutical compositions comprising the extract of the present invention may further comprise suitable carriers, excipients and diluents commonly used in the manufacture of pharmaceutical compositions.

Pharmaceutical dosage forms of the extracts of the present invention may be used in the form of their pharmaceutically acceptable salts, or may be used alone or in combination with other pharmaceutically active compounds, as well as in any suitable collection.

Pharmaceutical compositions comprising extracts according to the invention, in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols and the like, oral preparations, suppositories and sterile injectable solutions, respectively, according to conventional methods. Can be formulated and used. Carriers, excipients and diluents that may be included in the composition comprising the extract include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate , Cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. When formulated, diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents, and surfactants are usually used. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and the solid preparations may include at least one excipient such as starch, calcium carbonate, sucrose in the extract. ) Or lactose, gelatin and the like are mixed. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Oral liquid preparations include suspensions, solvents, emulsions, and syrups, and may include various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin. . Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, suppositories. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate and the like can be used. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin butter, glycerogelatin and the like can be used.

The composition of the present invention provides a cosmetic composition for inhibiting and improving wrinkle formation, comprising a dry extract, a non-polar solvent soluble extract obtained according to the preparation method or a compound separated therefrom as an active ingredient.

The composition containing the dry extract of the present invention can be used in various ways, such as cosmetics, face wash and shampoo for the skin anti-aging effect. Examples of products to which the present composition can be added include cosmetics such as various creams, lotions, and skins, and shampoos, rinses, cleansing agents, face washes, soaps, treatments, and cosmetic liquids.

Cosmetics of the present invention comprises a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids and seaweed extract. Components included in the cosmetic composition of the present invention may include components commonly used in cosmetic compositions in addition to the extract or compound as an active ingredient, for example, such as stabilizers, solubilizers, vitamins, pigments and flavorings Phosphorus aids and carriers.

The anti-aging cosmetic composition of the present invention can be prepared in any formulation commonly prepared in the art, for example, emulsion, cream, lotion, pack, foundation, lotion, essence, hair cosmetics and the like.

Preferred dosages of the extracts of the present invention vary depending on the condition and weight of the patient, the extent of the disease, the form of the drug, the route of administration and the duration, and may be appropriately selected by those skilled in the art. However, for the desired effect, the extract of the present invention is preferably administered at 0.0001 to 100 mg / kg, preferably at 0.001 to 100 mg / kg. Administration may be administered once a day or may be divided several times. The dosage does not limit the scope of the invention in any aspect.

The extract of the present invention can be administered to mammals such as mice, mice, livestock, humans, etc. by various routes. All modes of administration can be expected, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intrauterine dural or intracerebroventricular injection.

The present invention provides a health functional food comprising the extract and a food acceptable food supplement additive exhibiting an antioxidant effect. Examples of the food to which the extract may be added include various foods, beverages, gums, teas, vitamin complexes, and health functional foods.

It may also be added to foods or beverages for the purpose of antioxidant effects. At this time, the amount of the extract in the food or beverage may be added in 0.01 to 15% by weight of the total food weight, the health beverage composition may be added in a ratio of 0.02 to 5g, preferably 0.3 to 1g based on 100ml. .

The health functional beverage composition of the present invention is not particularly limited to other ingredients except for having the extract as an essential ingredient in the indicated ratio, and may contain various flavors or natural carbohydrates, etc. as additional ingredients, as in general beverages. Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; And conventional sugars such as polysaccharides such as dextrin, cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (tauumatin, stevia extract (for example, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The proportion of natural carbohydrates is generally about 1-20 g, preferably about 5-12 g per 100 ml of the composition of the present invention.

In addition to the above, the extract of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, coloring and neutralizing agents (such as cheese and chocolate), pectic acid and salts thereof, alginic acid and its Salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. In addition, the extracts of the present invention may contain flesh for the production of natural fruit juices and fruit juice beverages and vegetable beverages. These components can be used independently or in combination. The proportion of such additives is not so critical but is usually selected in the range of 0 to about 20 parts by weight per 100 parts by weight of the extract of the present invention.

Hereinafter, the present invention will be described in detail by the following Examples and Experimental Examples.

However, the following Examples and Experimental Examples are only illustrative of the present invention, and the content of the present invention is not limited by the following Experimental Examples.

Example 1 Preparation of Dried Methanol Extract

After drying by lyophilizing the dry, 100% methanol corresponding to 10 times the weight of the dry sample was added and extracted using a magnetic stirrer for 12 hours. After filtration under reduced pressure to separate the extract and the residue, 100% methanol corresponding to 5 times the dry weight at the start of extraction was added to the obtained residue and extracted using a magnetic stirrer for 6 hours. After filtration under reduced pressure again, 100% methanol corresponding to three times the dry weight at the start of extraction was added to the obtained residue, followed by extraction using a magnetic stirrer for 3 hours, followed by filtration under reduced pressure and concentration to obtain a final dried methanol extract ( Yield 17.6%).

Example 2. Preparation of Dried Acetone Extract

In Example 1, the extraction solvent was replaced with acetone, and acetone acetone extract was obtained through a process similar to that of Example 1 (yield 19.8%).

Example 3. Preparation of Dry Ethanol Extract

In Example 1, the extraction solvent was replaced with ethanol, and a dry ethanol extract was obtained through a process similar to that of Example 1 (yield 18.9%).

Example 4. Preparation of Dry Water Extract

In Example 1, the extraction solvent was replaced with water, and a dried water extract was obtained through a process similar to that of Example 1 (yield 16.4%).

Experimental Example 1. Determination of antioxidant activity by in vitro DPPH method

DPPH method (Blois, MS: Antioxidant determination by the use of a stable free radical, Nature , 26, 1199-1200, 1958) was used to determine the in vitro antioxidant effect of the extract.

DPPH (1,1-diphenyl picrylhydrazyl) method is a biological activity showing the antioxidant activity of tocopherol, ascorbate, flavonoid compounds, aromatic amines, Maillard browning products, peptides, etc. As a standard, L-ascorbic acid was used as a standard product to measure the antioxidant effect according to the degree of dark purple color fading by reduction by a substance. In each sample, 2 x 10 ^-4 M DPPH was added and absorbance was measured at 517 nm. Was measured.

As a result, DPPH free radical scavenging ability of dry water, methanol, 70% ethanol and acetone extract was 89.9%, 93.1%, 92.0% and 93.3% at 0.05 g / L concentration, respectively. Acid activity was found to be 96.3% (see Table 1).

Samples and Standards       DPPH free radical scavenging ability Water extract 89.9% Methanol extract 93.1% 70% Ethanol Extract 92.0% Acetone extract 93.3% L-ascorbic acid 96.3%

On the other hand, the hydrogen donating ability using the DPPH for each concentration of the whole dry, skin and flesh methanol extract is shown in FIG. DPPH free radical scavenging activity of the extracts was 94.1%, 93.4%, and 5.9%, respectively, at the concentration of 0.05 g / L, and the activity of L-ascorbic acid, which was used as a standard, was 95.9%. . DPPH free radical scavenging ability of total dry methanol extract was 73.6% and 21.2% at 0.025 and 0.01 g / L, respectively, and DPPH free radical scavenging ability of dry skin methanol extract was 72.3% at 0.025 and 0.01 g / L, respectively. , 32.3%, the dry antioxidant was found to be concentrated in the shell.

Experimental Example 2. Geriatric Disease and Anti-Aging Experiment

In order to confirm the therapeutic efficacy of the anti-aging of the extracts of the present invention was carried out the following experiment.

The experimental animals used in this animal experiment were 4 weeks old Sprague-Dawley rats (120 ± 10g ♂, Daegu Hyochang Science) for adult disease and anti-aging experiments. , The experimental group was administered the supernatant of the dried extract of the present invention instead of water and then tested for the tonic effect using blood.

The reagents used in this experiment were all first-grade or higher reagents, and related enzyme reagents were measured using sigma-express reagents.

2-1. Collection and Separation of Blood

After anesthetizing the experimental animals with ether, blood was collected from the heart, left for 2 hours at low temperature, centrifuged at 700xg for 10 minutes, and the supernatant was treated with 0.05 ml of CBC (1.0 mL) per 1.0 ml of blood. Cell count (green cross) was used for analysis while cryopreservation at -70 ℃.

2-2. Determination of Reactive Oxygen and Lipid Peroxide

The measurement of hydroxy radicals, known as the most powerful free radicals among free radicals, is a measure of the degree of hydroxyl radical generation to the extent of deoxyribose destruction. Ribose is destroyed to form an aldehyde, which is measured according to the method of literature (Halliwell B and Gutteridge JMC, FEBS. Lett., 128, pp347-350, 1981) , which is developed by reaction with thiobarbituric acid in an acid solution . It was.

The content of lipid peroxide (LPO) in serum known to be produced by these oxygen radicals is measured as described in Choi JH and Yu BP, AGE, 13, pp 61-64, 1990. It was.

As a result of examining the effect of the natural dry extract on the anti-aging effect by administering the extract of the present invention to the experimental animal SD rats for 3 weeks, the production of lipid peroxide (LPO) of the dried extract administration group was compared as shown in Table 2. 19.8% of the group effectively inhibited the production of lipid peroxide. In addition, it can be seen that the effect of the dry extract administration on the production of hydroxy radicals, the most powerful active oxygen among free radicals, is significantly inhibited by 23.4%.

Item Control group Dry extract Compared to the control group Lipid Peroxide (LPO) nmol / ml Serum 2.32 ± 0.20 1.86 ± 0.09 -19.8%  Hydroxy radical (-OH) nmol / mg protein 2.99 ± 0.26 2.29 ± 0.12 -23.4% Superoxide Dismutase (SOD) unit / mg Protein 0.89 ± 0.06 1.00 ± 0.07 + 12.4% Glutathione Peroxidase (GSHPx) IU / mg Protein 46.14 ± 2.71 53.59 ± 2.94 + 16.2%

2-3. Determination of Superoxide Dismutase Activity

Determination of superoxide dismutase (SOD) activity, which is important as an enzyme for removing reactive oxygen species, was quantified according to the method of Reference (Oyanagui, Y, Anal. Biochem., 42, pp290-296, 1984). In addition, the activity of glutathione peroxidase (GSHPx) in serum was measured as a method for measuring the reduction of NADPH at 340 nm as an important scavenger in vivo (Lawrence, RA and Burk, RF, Lipid, 19, pp444). -452, 1978).

In addition, when comparing the administration effect of the dried extract of the present invention on the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSHPx) as these active oxygen scavenging enzymes, 12.4% and 16.2%, respectively, were significant. It can be seen that the activity of SOD and GSHPx is increased. Due to the above results, it was confirmed that the administration of the dry extract can effectively prevent and prevent the aging process.

Experimental Example 3. Acute Toxicity Test

Acute toxicity test was performed using 6-week-old specific pathogen-free (SPF) SD rats. Two animals of each group were orally administered with the dry extract of the present invention at a dose of 100 mg / kg. After administration of the test substance, mortality, clinical symptoms, and changes in body weight were observed, and hematological and hematological examinations were performed.

As a result, no significant clinical symptoms or dead animals were noted in all animals treated with the test substance, and no toxic changes were observed in weight changes, blood tests, blood biochemical tests, and autopsy findings. As a result, the dry extract of the present invention did not show a toxicity change in rats up to 100 mg / kg, respectively, and the minimum lethal dose (LD ₅₀ ) was determined to be a safe substance of 100 mg / kg or more.

Formulation examples of the pharmaceutical composition and the preparation of the cosmetic composition containing a dry extract of the present invention will be described, but the present invention is not intended to limit this, but is intended to explain in detail only.

Formulation Example 1 Preparation of Powder

Dried Extract Powder 20 mg

Lactose 100 mg

Talc 10 mg

The above ingredients are mixed and filled in an airtight cloth to prepare a powder.

Formulation Example 2 Preparation of Tablet

10 mg dry powder

Corn starch 100 mg

Lactose 100 mg

2 mg magnesium stearate

After mixing the above components, tablets are prepared by tableting according to a conventional method for preparing tablets.

Formulation Example 3 Preparation of Capsule

10 mg dry powder

3 mg of crystalline cellulose

Lactose 14.8 mg

Magnesium Stearate 0.2 mg

According to a conventional capsule preparation method, the above ingredients are mixed and filled into gelatin capsules to prepare capsules.

Formulation Example 4 Preparation of Injection

10 mg dry powder

Mannitol 180 mg

Sterile distilled water for injection 2974 mg

Na ₂ HPO _{4 12} H ₂ O 26 mg

According to the conventional method for preparing an injection, the amount of the above ingredient is prepared per ampoule (2 ml).

Formulation Example 5 Preparation of Liquid

Dried Extract Powder 20 mg

10 g of isomerized sugar

5 g of mannitol

Purified water

After dissolving each component in purified water according to the usual method of preparing a liquid solution, adding lemon flavor appropriately, mixing the above components, adding purified water, adjusting the whole to 100 ml by adding purified water, and then filling into a brown bottle. The solution is prepared by sterilization.

Formulation Example 6 Preparation of Healthy Food

Dried Extract Powder 1000mg

Vitamin Mixture

70 μg of Vitamin A Acetate

Vitamin E 1.0 mg

Vitamin B ₁ 0.13 mg

Vitamin B ₂ 0.15 mg

Vitamin B ₆ 0.5 mg

0.2 μg of vitamin B ₁₂

Vitamin C 10 mg

10 μg biotin

Nicotinic Acid 1.7 mg

Folate 50 ㎍

Calcium Pantothenate 0.5mg

Mineral mixture

Ferrous Sulfate 1.75 mg

Zinc Oxide 0.82 mg

Magnesium carbonate 25.3 mg

Potassium monophosphate 15 mg

Dibasic calcium phosphate 55 mg

Potassium Citrate 90 mg

Calcium Carbonate 100 mg

Magnesium chloride 24.8 mg

Although the composition ratio of the above-mentioned vitamin and mineral mixtures is mixed with a component suitable for a health food in a preferred embodiment, the compounding ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional health food manufacturing method. The granules may be prepared and used for preparing a health food composition according to a conventional method.

Formulation Example 7 Preparation of Healthy Drink

100 mg dry powder

15 g of vitamin C

100 g of vitamin E (powder)

Iron lactate 19.75 g

3.5 g of zinc oxide

Nicotinamide 3.5 g

0.2 g of vitamin A

0.25 g of vitamin B ₁

0.3 g of vitamin B ₂

Water quantity

After mixing the above components in accordance with a conventional healthy beverage production method, and stirred and heated at 85 ℃ for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2 L container, sealed sterilization and then refrigerated and stored in the present invention For the preparation of healthy beverage compositions.

Although the composition ratio is mixed with a component suitable for a favorite beverage in a preferred embodiment, the composition ratio may be arbitrarily modified according to regional and ethnic preferences such as demand hierarchy, demand country, and usage.

Preparation Example 1 Softening Lotion (Skin) in Cosmetics Containing Dried Extract

Table 3 shows an example of the preparation of the flexible lotion (skin) in the cosmetic containing the extract.

number Raw material (wt%) Formulation example Comparative formulation example One Dry extract 2.0 - 2 glycerin 3.0 3.0 3 Butylene Glycol 2.0 2.0 4 Propylene glycol 2.0 2.0 5 Polyoxyethylene Cured Perm Oil 1.0 1.0 6 ethanol 10.0 10.0 7 Triethanolamine 0.1 0.1 8 antiseptic a very small amount a very small amount 9 Pigment a very small amount a very small amount 10 Spices a very small amount a very small amount 11 Purified water Remaining amount Remaining amount

<Production method>

Formulation example: 2, 3, 4, and 8 in 11 are added in order to dissolve it, and then dissolved by heating 5 times at about 60 ° C., and 10 times are added to 11 for stirring. Finally, 1, 6, 7 and 9 are added and sufficiently stirred, and then aged through a microfluidizer.

Comparative Formulation Example: No. 2, 3, 4 and 8 were added to 11 in order to dissolve by stirring. 5 was heated to 60 ° C for dissolution and 10 times were added to 11 for stirring. Finally, 6, 7, and 9 were added and sufficiently stirred before passing through a microfluidizer and aged.

As described above, the dry extract of the present invention shows an excellent antioxidant effect, and improves the effects of cancer, arteriosclerosis, aging, diabetes, and diabetes complications caused by cell damage caused by free radicals. Indicates.

1 is a diagram showing the antioxidant activity of each concentration of the whole dried, dried skin, dried fruit pulp extract.

Claims (7)

Composition containing an extract of Saururus chinensis BAILL. Having an antioxidant effect as an active ingredient. The composition of claim 1, wherein the extract is water and C ₁ to C ₄ lower alcohols such as methanol, ethanol, butanol and the like, or a mixture thereof or an extract that is soluble in acetone. The composition of claim 1, wherein the composition is a pharmaceutical composition comprising a pharmaceutically acceptable carrier and an excipient or a cosmetic composition comprising a cosmetically acceptable base. 2. The composition of claim 1, wherein the broom is a broom husk. The composition of claim 1 comprising from 0.02 to 50% by weight of a dry extract relative to the total weight of the composition. A dietary supplement comprising a blight extract exhibiting an antioxidant effect and a food acceptable additive. The health functional food of Claim 6 which is a health drink.