KR20110019324A - Bokbun free oil composition, its use and preparation method - Google Patents

Abstract

The present invention relates to bokbunja oil composition containing conjugated linoleic acid, omega-3, omega-6, ellagitannins, ellagic acid, palmitic acid, stearic acid, oleic acid And it relates to a composition comprising bokbunja oil further containing at least one component selected from the group consisting of linoleic acid, uses thereof and a method of preparing the same.

Description

Bokbun free oil composition, use and preparation method thereof Rubus coreanus Miquel oil composition, use and preparation method

The present invention relates to a bokbunja oil composition containing conjugated linoleic acid, its use and preparation method, wherein the composition is omega-3, omega-6, ellagitannins, ellagic acid, palmitic acid It may further contain one or more components selected from the group consisting of stearic acid and oleic acid, and have antioxidant, anti-aging, anti-obesity or anti-atopic effects. It is also an object of the present invention to provide a method for obtaining bokbunja oil containing a high content of an active substance.

Bokbunja ( Rubus coreanus Miquel, Korean black berry) belongs to the family Rosaceae, and in synonymous gagam is known to have interpolation, nominal, diuretic, energetic enhancement. Recently, bokbunja fruit has been used a lot of additives or casting of food or beverage.

Competitive cultivated cultivated in Korean farms are mainly sold as frozen fruit or as a concentrated liquid.In order to increase the yield of raw liquid and to sterilize at the same time, small farming cooperatives put water: bokbunja at a ratio of 1: 1 in tank facilities. Boil at a temperature higher than ℃ to extract hot water. However, this hot water extraction method has a problem that destroys the vitamin component in the bokbunja. In addition, most of the residues including bokbunja are discarded.

Generally, pharmaceutical compositions in which water or organic solvent extracts of bokbunja are used for anticancer and antidepressant treatment have been reported (Korean Patent Laid-Open Publication Nos. 10-2005-0113290 and 10-2007-0108724, respectively). It does not suggest ellagic acid or the like having antioxidant activity, nor does it disclose any bokbunja seeds.

In addition, Korean Patent Application No. 10-2006-0033340 has a limitation on the invention of separating antioxidant active substances from bokbunja, or the use of hot water extraction method, and the use of bokbunja seeds and the types of antioxidant substances of the present invention. It is not starting.

Thus, the present inventors first discovered that a large amount of conjugated linoleic acid is present in bokbunja oil extracted from bokbunja seeds during the study of bokbunja antioxidant organic active ingredient, in addition to the presence of a large amount of active ingredients such as ellagitannins and allegic acid It was found that the present invention was completed by deriving an extraction method which minimizes the destruction of these active ingredients.

Accordingly, the present invention is to provide a bokbunja oil composition containing conjugated linoleic acid, its use and preparation method, the composition is omega-3, omega-6, ellagitannins, ellagic acid And bokbunja oil further containing at least one component selected from the group consisting of palmitic acid, stearic acid and oleic acid.

In addition, the present invention is to provide a bokbunja oil containing a high content of a substance having antioxidant, anti-aging, anti-obesity or anti-atopic activity by extracting bokbunja oil from bokbunja cultivated in Korea. It is also an object of the present invention to provide a method for extracting bokbunja oil containing a high content of the active ingredient from bokbunja seed.

The present invention is characterized by a composition comprising bokbunja oil containing conjugated linoleic acid.

In addition, the present invention further contains at least one component selected from the group consisting of omega-3, omega-6, ellagitannins, ellagic acid, ellagic acid, palmitic acid, stearic acid and oleic acid. By providing a bokbunja oil composition, it is possible to provide a composition excellent in antioxidant, anti-aging, anti-obesity or anti-atopic effect.

In addition, by performing one or more extraction methods selected from the group consisting of cold compression method, hot compression method, low temperature compression method and solvent extraction method of the bokbunja seed to extract the bokbunja oil containing conjugated linoleic acid (CLA) by Bokbun free oil contained in this high content can be obtained.

In addition, the present invention can provide a health functional food, food, cosmetics, antioxidants, aging or atopic prophylaxis and treatment, including the conjugated linoleic acid (CLA) containing bokbunja oil composition, obesity prevention and treatment have.

The present invention extracts bokbunja oil containing a high content of antioxidant, anti-aging, anti-obesity or anti-atopic activity from bokbunja, it can be used for the prevention and treatment of diseases caused by oxidation, atopic diseases, etc. After extracting the juice from the conventional bokbunja and extracting the essential oil component having an antioxidant activity from the bokbunja seed that was discarded with the residue has the advantage of reducing the resource waste.

1 is a result of analyzing the fatty acid components in the conventional bokbunja hexane extract (bokbunja A), Bokbunja oil (bokbunja B) and grape seed oil of Example 2 [PA: palmitic acid, ST: stearic acid, OA: oleic acid, LA : Linoleic acid, LNA: linolenic acid, CLA: conjugated linoleic acid].
2 shows the results of DPPH analysis.
Figure 3 shows the results of the MTT experiment.
Figure 4 shows the results of the MMP-1 inhibitory effect experiment.
Figure 5 shows the results of measuring the β-hexamiminidase release rate of 10 μM of beta hexoamidase inducer (A23187), Bokbunja oil concentration of Example 2 and the control group.

The present invention relates to a bokbun free oil composition containing conjugated linoleic acid (CLA). In the present invention, the content of conjugated linoleic acid is 5 to 30% by weight based on the total fatty acid content in bokbunja oil, more specifically, the content may be 10 to 25% by weight.

In the present invention, the composition adds one or more components selected from the group consisting of omega-3, omega-6, ellagitannins, ellagic acid, ellagic acid, palmitic acid, stearic acid and oleic acid It may contain bokbunja oil containing, and may also contain protein, dietary fiber.

In particular, the bokbunja oil composition of the present invention is 5 to 30% by weight of conjugated linoleic acid, 0.8 to 3% by weight of palmitic acid, 0.5 to 1.5% by weight of stearic acid, 5 to 15% by weight of oleic acid, 35 to 65% by weight of linoleic acid, linolenic acid 15 It is more preferable to contain -35 weight%.

In the present invention, the bokbunja oil can be extracted from the bokbunja seed, in particular bokbunja oil can be separated from the bokbunja seeds in the remaining residue after bokbunja juice.

Conjugated linoleic acid has been reported for anti-cancer, anti-arteriosclerosis, anti-obesity, anti-inflammatory and anti-oxidant activity. Especially, it is known that it helps to inhibit mast cell differentiation and decrease body fat mass. This is the first time.

Omega-3 is also called omega-3 fatty acid and belongs to unsaturated fatty acid. α-linolenic acid (ALA), eicosaptanoic acid (EPA) and docosahexaenoic acid (DHA) components and the like. It is known as an effective ingredient for antioxidant activity, anticancer activity, prevention or treatment of cardiovascular disease, enhancement of immunity, improvement of brain function, prevention or treatment of degenerative arthritis.

Omega-6 is also called omega-6 fatty acid and includes linoleic acid, gamma-linoleic acid, arachidonic acid components and the like. It is known to be involved in the biosynthesis of cell membranes, physiological control of blood vessels, immune system, etc., and it lowers the cholesterol level in the blood to lower the risk of heart disease, immune enhancing effect, blood sugar control effect, obesity prevention effect, and aging prevention. It is effective in maintaining skin health.

Ellagitannins are known as one of a group of ingredients having an antioxidant effect, liver function improvement effect, anticancer effect, skin protection effect and anti-allergic effect.

Ellagic acid (ellagic acid) consists of four rings of polyphenols, which are present in the plant in the form of ellagitannins. It has antioxidant, antiviral, antimutagenic and anticancer functions and is known to inhibit cancer cell activity in breast and esophagus, skin, colon, prostate and pancreas.

In the present invention, the composition has antioxidant, anti-aging or anti-atopic efficacy. In addition, since the anti-obesity effect of CLA is known, it may also include an anti-obesity effect.

The present invention also relates to a composition extracted from bokbunja oil and comprising conjugated linoleic acid (CLA), wherein the composition has antioxidant, anti-aging, anti-obesity or anti-atopic efficacy.

The present invention relates to a method for extracting bokbunja oil containing conjugated linoleic acid (CLA) by performing one or more extraction methods selected from the group consisting of cold pressing, hot pressing, low temperature pressing and solvent extraction.

In the present invention, the cold pressing method is a method of directly compressing the seed flowing out when heat is applied without obtaining heat treatment to obtain oil, and when the heat is applied, pigments, fragrances, gums, etc. flow out into the oil and the color of the oil is increased. Because it is thick and sometimes tastes bad, it is a method of immediately squeezing a crushed product without heat treatment in order to obtain good oil. The hot pressing method is a method of steaming and squeezing after roasting at a high temperature of 100 to 250 ° C. It is a method of squeezing oil by compressing as follows. In addition, the said solvent extraction method is a method of extracting using solvents, such as n-hexane.

In the present invention, the bokbunja ( Rubus 5 to 100 parts by weight of water per 10 parts by weight of coreanum fruit is juiced to obtain a juice, and the residue after the juice can be further compressed to obtain juice. The obtained juice was concentrated under reduced pressure to remove the solvent component, dried to separate the bokbunja seeds and separated using a sieve, etc., centrifugation can be separated only bokbunja seeds. The bokbunja seed may be pressed for 5 to 40 minutes at 80 to 150 ° C. (more preferably, 100 to 130 ° C.) using an oil rubber or expeller type milking machine to obtain bokbunja oil containing conjugated linoleic acid.

Bokbun-free oil composition comprising conjugated linoleic acid of the present invention is a substance derived from natural products with little toxicity and side effects.

The present invention provides an agent for preventing and treating aging or atopy, an antioxidant, and an agent for preventing and treating obesity, containing the bokbunja oil composition as an active ingredient.

The medicaments according to the invention can be used in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols and the like, oral formulations, external preparations, suppositories and sterile injectable solutions, respectively, according to conventional methods. have. Carriers, excipients, and diluents that may be included in compositions comprising bokbunja oil include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, aceitol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate , Cellulose, methyl cellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, and mineral oil. When formulated, diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents, and surfactants are usually used. Solid form preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, which include at least one excipient such as starch, calcium carbonate, sucrose or lactose, It is prepared by mixing gelatin. In addition to simple excipients, lubricants such as magnesium stearate and talc may also be used. Liquid preparations for oral administration include suspensions, solvents, emulsions, and syrups, and may include various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin. have. Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, suppositories. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oils such as olive oil, injectable esters such as ethyl oleate and the like can be used. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin butter, glycerogelatin and the like can be used. In addition, it may be administered in the form of external skin preparations such as ointments, liquids, patches, dressings, sprays and the like that can be topically applied topically.

The preferred dosage of the conjugated linoleic acid-containing bokbunja oil composition according to the present invention depends on the condition and weight of the patient, the extent of the disease, the form of the drug, the route and duration of administration, and may be appropriately selected by those skilled in the art. However, for the desired effect, the bokbunja oil composition of the present invention is preferably administered at 0.0001 to 200 mg / kg, preferably 0.001 to 100 mg / kg per day. Administration may be administered once a day or may be divided several times. The dosage does not limit the scope of the invention in any aspect.

In addition, the present invention provides a health functional food comprising the bokbunja oil composition as an active ingredient. In addition to the active ingredient, a food acceptable additive may be added. The health functional food includes, for example, various foods, beverages, gums, teas, vitamin complexes, and health functional foods.

At this time, the amount of Bokbunja oil in the health functional food may be added to 0.01 to 20% by weight of the total food weight, the health beverage composition may be added in a ratio of 0.02 to 15 g, preferably 5 to 12 g based on 100 ml. .

The health functional beverage composition of the present invention has no particular limitation on the other components besides containing the bokbunja oil composition as essential ingredients in the indicated proportions, and may contain additional ingredients such as various flavoring agents or natural carbohydrates as in general beverages. Can be. Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; And conventional sugars such as polysaccharides such as dextrin, cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As a flavoring agent other than the above-mentioned, a natural flavoring agent, taumartin, a stevia extract, for example, rebaudioside A, glycyrrhizin, etc .; And synthetic flavoring agents such as saccharin, aspartame and the like. The proportion of said natural carbohydrates is generally about 1 to 2 g, preferably about 1 to 1.5 g, per 100 ml of the composition of the present invention.

In addition to the above, the bokbunja oil composition of the present invention is various nutrients, vitamins, minerals (electrolytes), synthetic and natural flavors, coloring and neutralizing agents (cheese, chocolate, etc.), pectic acid and salts thereof, alginic acid and salts thereof, organic acids, Protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages and the like. In addition, the bokbunja oil of the present invention may contain a fruit flesh for the production of natural fruit juice and fruit juice beverage and vegetable beverage. These components can be used independently or in combination. At this time, the ratio of the additive is not very important but is generally selected from the range of 0.1 to about 30 parts by weight per 100 parts by weight of bokbunja oil of the present invention.

The present invention also provides a cosmetic containing the bokbunja oil composition as an active ingredient.

In the case of the cosmetics, Bokbun Free oil based cosmetic products (cosmetics, creams, essences, cleansing foam, cleansing water, packs, soaps), body products cosmetics (body lotion, body oils, body gels, soaps), color cosmetics (foundation) , Lipstick, mascara, makeup base), hair cosmetics (shampoo, rinse, hair conditioner, hair gel) and the like can be used in combination with 0.05 to 20.0% by weight relative to the dry weight of the cosmetic.

Hereinafter, the present invention will be described in more detail through examples according to the present invention and comparative examples not according to the present invention, but the scope of the present invention is not limited to the examples given below.

< Production Example  1> Bokbunja Seed Separation

The bokbunja fruit grown in North Jeolla Province Gochang region (Rubus 1 kg of coreanum fruit) was put in 1 kg of water and first juiced at room temperature for 2 hours to obtain 800 g of juice. The residue after the first juice was put into a squeezer and compressed to obtain 100 g of bokbunja concentrate.

The residues after the first juice and the residues after compression were collected and concentrated under reduced pressure with a vacuum rotary concentrator to remove the solvent components, followed by hot air drying to separate the bokbunja seeds, which were placed in a sieve vibrator and shaken on a sieve of 0.1 mm in diameter. Into bokbunja seeds were separated by centrifugation at 3000 rpm for 20 minutes (100 g).

< Example  1> Bokbun Free  Produce

20 kg of Bokbunja seeds were placed in an XPeller type milking machine equipped with a temperature sensor and preheated with the thermostat set at 120 ° C. Bokbunja seed was pushed into the hopper and the milking machine was pressed for 20 minutes to extract 2 kg of Bokbunja oil containing conjugated linoleic acid (yield 10%).

< Example  2> Bokbun Free - Hexane  Extract manufacturer

20 kg of bokbunja seeds were ground with a grinder at 3000 rpm, and n-hexane was added thereto and shaken well. After 24 hours, the mixture was concentrated under reduced pressure with a vacuum condenser for 1 hour at 45 ° C., followed by evaporation to dryness, to obtain 1.9 kg of bokbunja oil hexane extract containing conjugated linoleic acid (yield: 9.5%).

< Experimental Example  1> Bokbun free  Ingredient analysis

Existing Bokbunja hexane extract [Bioresource Technology 99 "Characterization of seed oils from fresh Bokbunja (Rubus coreanus Miq.) And wine processing waste" (2008) 2852-2856, Comparative Example 1], Bokbunja oil obtained in Example 1, Example Component analysis was performed using gas chromatography on bokbunja free-hexane extract and grape seed oil obtained in 2, the results are shown in Table 1 and FIG.

Detailed gas chromatography conditions and experimental procedures are as follows:

The column used for fatty acid analysis was a Supelcowax-10 fused silica capillary column (100 m × 0.25 mm id, 0.2 μm film thickness; Supelco, Inc., Bellefonte, PA, USA) Was used and the gas velocity (helium) was flowed at 1.2 mL / min.

The temperature of the column oven was raised by 4 ℃ per minute from 190 ℃ to 240 ℃. The temperature of the injector and detector of the sample was set at 260 ° C. 1 μL of the sample was added and the sample sample was set to enter 1 / 500,000 column.

Result of ingredient analysis of Bokbun free oil Division (weight%) Comparative Example 1 Example 1 Example 2 Palmitic acid (C16: 0) 1.55 ± 0.04 1.92 1.97 ± 0.03 Palmitic Acid - 0.05 - Stearic acid (C18: 0) 0.65 ± 0.03 0.81 0.81 ± 0.05 Oleic Acid (C18: 1) 8.04 ± 0.2 8.95 8.95 ± 0.15 Linoleic Acid (C18: 2) 53.18 ± 1.03 42.84 52.84 ± 0.90 Linolenic Acid (C18: 3) 29.36 ± 0.53 20.6 ± 0.34 20.09 ± 0.34 Gamma-linoleic acid - 0.03 - 9,11-CLA - 24.8 14.72 ± 0.02

< Experimental Example  2> antioxidant activity experiment

end. DPPH Analysis

DPPH analysis was performed to measure the antioxidant activity of bokbun free oil. In order to measure the antioxidant activity according to the concentration of bokbunja oil, ascorbic acid 100μg / mL, bokbunja seed oil of Example 1 10μg / mL, 100μg / mL, 1000μg / mL, bokbunja seed oil (hexane extraction) of Example 2 10μg / Samples were prepared at mL, 100 μg / mL, and 1000 μg / mL, and 10 μL of the sample was added to a 96-well plate, followed by 190 μL of DPPH (1,1-diphenyl-2-picrylhydrazyl) reagent. . Thereafter, absorbance was measured at ELISA 515 nm.

As a result, DPPH decreased by more than 20% in most samples (see FIG. 2).

I. Oxygen Radical Absorbance Capacity (ORAC) value measurement

The result of measuring the active oxygen scavenging ability of Bokbun free oil is as shown in Table 2, it can be seen that the active oxygen scavenging ability is excellent.

Specimen ORAC value (μmol TE / 100g) Control (strawberry) 3,577 Example 1 (Bokbun Free Oil Extract) 5,347 Broccoli 3,083

< Experimental Example  3> anti-aging experiment

end. MTT Experiment

In experiments measuring cell viability, yellow tetrazolium salt MTT is reduced to water-insoluble formazan crystals in living cells, which are later dissolved by DMSO. The amount of crystals produced was measured by the spectroscopic method. As a result, the larger the number of living cells, the greater the generation of these crystals, and thus, the viability of the cells can be easily measured through this method.

First, human dermal fibroblast (HS68) cells were spread on a 12-well plate at 2 X 10 ^5, and 400 ul of MTT solution was added to each well, and placed in a 37 ° C. CO ₂ incubator for 2 and a half hours and 3 hours (bokbunja oil extract). Was tested at concentrations of 0, 1, 5, 10, 50, 100 μg / mL). Thereafter, the medium was removed and 500 ul of DMSO was added thereto. After mixing well by pipetting, absorbance was measured at 570 nm (see FIG. 3).

I. Inhibition of collagen degrading enzyme MMP-1 (Proteins of the matrix metalloproteinase)

Western blot was measured using a cell line, HS68, which is a human skin fibroblast, and a sample of bokbunja seed oil of Example 1 was used. Specifically, cells were sampled for 24 hours to collect the cells, the protein was quantified by the Bradford assay to determine the value to be loaded when running. Running was reduced to 85V on a stacking gel and then to 100V on a separating gel for about 2 hours. After that, 100V was transferred to a film by running the gel under conditions of 1 and a half hours and 110V and 1 hour. The transferred membrane was cut to an appropriate size in accordance with the MMP 54 to 55 kD size, and then blocked with a scheme milk for 1 hour. After that, the antibodies to MMP-1 in the scheme milk 1: 1000 and bound for at least 5 hours. After the procedure, the cells were washed three times with TBST for 10 minutes, and ANTI-mouse antibody was added for about 2 hours. After that, the mixture was washed three times for 10 minutes with TBST (mixture of tris-buffered saline and Tween 20), and the band was identified using a LAS machine.

As a result, it was shown that the effect of reducing MMP-1 in bokbunja freedom of Example 1 [see Fig. 4].

< Experimental Example  4> Anti-atopic dermatitis  Experiment -β- Hexamiminidase  Inhibitory effect measurement

The inhibitory effect of [beta] -hexamiminidase was confirmed using the sample of 5, 10, 25, 50, 100 micrograms / mL of Bokbunbun oil of Example 2 as follows.

Treatment of cells with A23187, a β-hexamiminidase inducer, introduces calcium ions into the cells and releases β-hexamiminidase into the medium.

P-NAG substrates were treated to bind secreted β-hexamiminidase enzyme.

The absorbance was measured at 400 nm by ELISA after 1 hour of treatment with a termination buffer (Na ₂ CO ₃ or NaHCO ₃ ) 0.1 mol hexaamide enzyme reaction [see FIG. 5].

As a result, β-hexamiminidase release was reduced by more than 30% at 100 μg / mL in bokbunja oil.

Formulation example  1: Manufacture of capsule

Bokbun free oil 10 mg of Example 1

3 mg of crystalline cellulose

Lactose 14.8 mg

Magnesium Stearate 2mg

According to a conventional capsule preparation method, the above ingredients were mixed and filled into gelatin capsules to prepare capsules.

Formulation example  4: Preparation of Injection

Bokbun free oil 10 mg of Example 1

Mannitol 180 mg

Sterile distilled water for injection 2794 mg

Na ₂ HPO ₄ 12H ₂ O 26 mg

Injectables were prepared at the above ingredient content per ampoule (2 mL) according to a conventional method of preparing injectables.

Formulation example  6: Preparation of Ointment

5 g of Bokbun free oil to conduct

20 g of cetyl palmitate

40 g of cetanol

40 g of stearyl alcohol

Myristan isopropyl 80 g

20 g of monostearate sorbitan

Polysorbate 60 g

1 g of paraoxybenzoic acid profile

1 g of methyl paraoxybenzoate

Phosphoric Acid and Purified Water Proper

An ointment was prepared from the above components according to a conventional ointment preparation method.

Formulation example  7: manufacture of health drinks

Bokbun free oil 10 mg of Example 1

15 g of vitamin C

100 g of vitamin E (powder)

Iron lactate 19.75 g

3.5 g of zinc oxide

Nicotinamide 3.5 g

0.2 g of vitamin A

0.25 g of vitamin B1

0.3 g of vitamin B2

Water quantity

After mixing the above components in accordance with a conventional method for preparing a healthy beverage, and then stirred and heated at 85 ℃ for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2 L container, sealed and sterilized and then refrigerated and stored in a healthy beverage Was prepared.

Although the composition ratio is a composition suitable for a preferred beverage in a preferred embodiment, the composition ratio may be arbitrarily modified according to regional and ethnic preferences such as demand hierarchy, demand country, and usage.

Formulation example  8: Preparation of Essence

Bokbun free oil 10.0 mg of conduct

Glycerin 3.0 mg

EDTA 0.05 mg

Benzophenone-9 0.04 mg

Carboxyvinyl Polymer 0.2 mg

Triethanolamine 0.18 mg

Oxidoteses-25 0.6 mg

Glyceryl Monostearate 1.0 mg

Preservative 0.01 mg

Fragrance 0.01 mg

Purified water

Essence was prepared by the composition as described above according to the conventional essence manufacturing method.

Claims (13)

Bokbun free oil composition comprising a conjugated linoleic acid (CLA).
The method of claim 1,
Bokbun free oil composition, characterized in that the content of conjugated linoleic acid 5 to 30% by weight based on the total fatty acid content in Bokbun free oil.
The method of claim 1,
The composition is a bokbunja oil further containing one or more selected from the group consisting of omega-3, omega-6, ellagitannins, ellagic acid, palmitic acid, stearic acid and oleic acid Composition.
The method of claim 1,
Bokbun free oil is characterized in that the extract from Bokbunja seed.
The method of claim 1,
Bokbunja oil composition characterized in that the composition has antioxidant, anti-aging or anti-atopic efficacy.
Conjugated linoleic acid (CLA) characterized by extracting bokbunja oil containing conjugated linoleic acid (CLA) by performing one or more extraction methods selected from the group consisting of cold pressing, hot pressing, low temperature pressing and solvent extraction Method of manufacturing bokbunja oil comprising a.
The method of claim 6,
The extraction method of the bokbunja free oil, characterized in that the compression for 5 to 40 minutes at 80 ~ 150 ℃ using an oil rubber or expeller type milking machine.
Health functional food comprising the bokbunja oil composition of any one of claims 1 to 5.
Food comprising the bokbunja oil composition of any one of claims 1 to 5.
Antioxidant containing the bokbunja oil composition of any one of Claims 1-5.
A medicament for preventing and treating aging or atopy, comprising the bokbunja oil composition of any one of claims 1 to 5.
A pharmaceutical for preventing and treating obesity, comprising the bokbunja oil composition of any one of claims 1 to 5.
Cosmetics containing the bokbunja oil composition of any one of claims 1 to 5.

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