JPH08333270A - Cell activator - Google Patents

Abstract

PURPOSE: To obtain the subject agent containing an extract of Trapa japonica Flerov., safe to the skin, having greatly high cell-activating effect, thus capable of maintaining fresh skin through its revitalization. CONSTITUTION: This cell activator contains an extract of Trapa japonica Flerov. The extraction is pref. accomplished by using ethanol, or its mixture with water. Specifically, for example, 300ml of a 50% aqueous ethanol solution is added to 10g of dry fruits of Trapa japonica Flerov. and the system is left to stand for 5 days while agitating it at times. The extract can be prepared into cosmetics of various formulations through incorporation of e.g. a liquid oil such as squalene, solid oil such as beeswax, activator, humectant such as glycerol., etc.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to cosmetics, foods or pharmaceuticals having a high cell activating effect.

[0002]

[Prior art]

[0003] Hishomi is called Trapa natans L, which is a member of the family Rhizophora, and is a dried fruit of a plant usually called a rhombus. Hishi are distributed in temperate to subtropical regions of Japan, Korea, and China, and are annual edible plants that grow on ponds and swamps. As a conventional application, it is used as a nutritional tonic and antipyretic agent. Furthermore, the present inventors have found that they have a whitening effect, a hyaluronidase activity inhibitory effect, and the like.

[0004]

SUMMARY OF THE INVENTION An object of the present invention is to obtain a cell activator which is safe when applied to the skin and which activates the skin and keeps youthful skin.

[0005]

Means for Solving the Problems The present inventors have found the effects on plants that are edible and have been confirmed to be safe in order to obtain a cell activator that activates the skin and maintains youthful skin. , Hishimi extract was found to have a very cell-activating effect. As a method for utilizing Hishimi, extraction is performed with a hydrophilic organic solvent such as ethanol, methanol, or acetone, or a mixed solvent of these and water. However, it is natural that extraction with ethanol or a mixed solvent of water and ethanol is preferable because it is extraction of cosmetic raw materials.
In some cases, a polyhydric alcohol such as glycerin, 1,3 butylene glycol, propylene glycol or the like or a mixed liquid of polyhydric alcohol and water can be used for extraction. Further, freeze-drying and using it as powder is also effective depending on the method of use.

[0006] This substance is used as other cosmetic raw materials, for example, liquid oils such as squalane and jojoba oil, solid oils such as beeswax and cetyl alcohol, various activators, moisturizing agents such as glycerin and 1,3 butylene glycol, and various agents. It can be added to adjust various dosage forms of cosmetics. For example, a lotion, a cream, a milky lotion, a pack, or the like may be used depending on the purpose.

[0007]

[Manufacturing Example] The following describes a manufacturing example which is a method for manufacturing an actual extract, but the present invention is not limited to this manufacturing example.

Production Example To 10 g of Hishimi fruit (dry product) was added 300 ml of a 50% ethanol aqueous solution, and the mixture was left for 5 days with occasional stirring.
This was filtered and freeze-dried.

Example-1 Lotion Olive oil 0.5 Extract of Production Example 0.5 Polyoxyethylene (20 EO) sorbitan monostearate 2.0 Polyoxyethylene (60 EO) hydrogenated castor oil 2.0 Ethanol 10.0 1.0% sodium hyaluronate aqueous solution 5.0 Purified water 80.0

Example-2 Cream A Squalane 20.5 Olive Oil 2.0 Mink Oil 1.0 Jojoba Oil 5.0 Beeswax 5.0 Cetostearyl Alcohol 2.0 Glycerin Monostearate 1.0 Sorbitan Monostearate 2. 0 Extract of Production Example 0.5 B Purified water 47.9 Polyoxyethylene (20 EO) sorbitan monostearate 2.0 Polyoxyethylene (60 EO) hydrogenated castor oil 1.0 Glycerin 5.0 1. 0% aqueous solution of sodium hyaluronate 5.0 Methyl paraoxybenzoate 0.1 A and B were weighed and heated to 70 ° C., A was slowly added to B while stirring, and then 30 while slowly stirring.
Cooled to ° C.

Cell activation test 1. Splitting and action 1) Fibroblasts cultured in culture flask (Dainippon Pharmaceutical /
The cell system Fb) was stripped with a trypsin-EDTA reagent (Kurabo), and a cell suspension was adjusted with PBS (-) (cell concentration 5 × 10 ⁵ cells / ml). 2) In a 24-well plate, 20% -FBS MEM medium and normal human fibroblasts 5000 cells / well
After planting and culturing in a CO2 incubator for 3 days, 3) remove the culture solution and replace with serum-free MEM
It was further cultured for 2 days. 4) 2 ml of sample solution prepared with serum-free MEM medium
To 10% FBS-MEM medium 0.1 ml
Was added (0.5% serum concentration in the sample). After culturing for 5 days, the amount of collagen and mitochondrial activity were measured. In addition, a well cultured only in MEM medium was used as a control.

2. Mitochondrial activity measurement method (MTT) 1) 1 ml of a sample was withdrawn from the culture plate and transferred to another plate to serve as a control for each well. 2) 0.1 ml of MTT reagent was added to each plate and incubated for 4 hours. 3) 1 ml of 20% SDS reagent containing 0.01N HCl
Was added and left overnight. 4) The absorbance at 570 nm was measured, and the mitochondrial activity (relative value) was calculated by the following formula. The mitochondrial activity (relative value per unit cell) is given by the following formula.

[0013]

[Table 1] The relative value with respect to the control was used. The test concentration is 5 ppm.

[0014]

[Effect] As described above, the Hishimi extract has a very high cell activating effect at a low concentration, and a cell activating agent having an action of activating the skin and maintaining youthful skin was obtained.

Claims (1)

[Claims] 1. A cell activating agent containing an extract of Hishimi