KR20040027283A - A method of vegiterble and marin collagen peptide - Google Patents

Abstract

PURPOSE: A process for preparing vegetable and marine collagen peptides is provided, thereby improving the stability of the collagen peptides because citric acid is used, improving the taste and flavor thereof, and preventing environment problems because water is used as a solvent. CONSTITUTION: A process for preparing vegetable and marine collagen peptides comprises the steps of: removing impurities from vegetables and marine products and chopping them; adding an organic acid solution into the vegetables and marine products; adding protease such as trypsin, papain or pepsin into the mixture of the organic acid solution and the vegetables and marine products; filtering the collagen peptide solution to remove sludgy therefrom when collagen concentration reaches to a certain value; heating the filtrated collagen peptide solution; and centrifuging the collagen peptide solution to remove impurities.

Description

A method of vegiterble and marin collagen peptide}

The present invention relates to a method for producing collagen peptides, and more specifically, to produce vegetable and marine collagen peptides by enzymatically lowering the molecular weight of plants and seafood such as fruits, vegetables, seeds, nuts, mushrooms, seaweeds, fish, fish, etc. It is about how to.

Collagen is a protein fiber in which the basic molecular units of three polypeptides, called tropocollagen, are twisted into fibers and arranged in parallel to each other as main components of animal skin or skin. The collagen is insoluble under neutrality, but the tropocollagen is insoluble in neutral, but soluble at certain salts and at low pH.

Typical collagen production method comprises the step of screening the raw pig skin (pig skin); pre-treatment step of treating the selected pig skin with acid; washing the excess chemicals with a large amount of water to facilitate the extraction from the raw material after the pre-treatment step Washing step ;, Extraction step of extracting collagen from the finished pig skin; Desalting step to remove toxic inorganic acids; Collagen manufacturing step of producing a water-soluble collagen using the extract; In order to increase the purity of collagen is made through a purification step of performing a desalination process as a purification process.

Therefore, the conventional method for producing collagen from pig skin requires an apparatus for treating wastewater generated in a pretreatment step and a washing process, and requires a separate desalting process to remove highly toxic inorganic acids used in acid treatment. There is a problem in that the yield efficiency is also poor by going through this complicated and several steps.

Korean Patent Laid-Open Publication No. 2001-0044115 proposes a method for preparing animal-soluble collagen using an enzyme to solve the above problems, but also does not completely solve the problems of the prior art, and is used to prepare collagen. It does not present a variety of.

In addition, in the situation where the avoidance of animal collagen is intensified due to weaknesses in use such as foot-and-mouth disease and mad cow disease, it is urgently required to develop a substitute that can replace animal collagen.

Therefore, not only the development of a manufacturing method that simplifies a complex manufacturing process including chemical treatment or heavy metal treatment is required, but also the need to diversify the raw materials for collagen production and the development of a manufacturing method for producing vegetable and marine collagen.

Accordingly, the present invention has been made to solve the above-described problems of the prior art, an object of the present invention is to diversify the raw materials for producing collagen and to prepare vegetable or marine collagen peptides in a simplified process than the prior art. The purpose is to provide a way.

1 is a process chart showing a process of the conventional collagen production method.

2 is a process chart showing a collagen production method according to the present invention.

The present invention for achieving the above object is a raw material pre-treatment step to remove and dope foreign materials of the raw material to form a raw material of a predetermined size; Immersing an organic acid solution in which an organic acid aqueous solution of the raw material is formed by adding an organic acid aqueous solution to the raw material; An enzyme treatment step of hydrolyzing the protein to form an aqueous collagen peptide solution by adding a specific proteolytic enzyme to the aqueous organic acid solution of the raw material; A first filtration step of forming a first filtered collagen peptide aqueous solution by removing sludge from the collagen peptide aqueous solution when collagen reaches a predetermined concentration in the collagen peptide aqueous solution; Enzymatic inactivation and sterilization step of heating the temperature of the primary filtration collagen peptide aqueous solution to a predetermined temperature or more, and deactivating and sterilizing the enzyme by maintaining the temperature for a specific time to form a sterilized collagen peptide aqueous solution. ; And a second filtration step of forming a second filtration collagen peptide aqueous solution by removing foreign matter from the sterilized collagen peptide aqueous solution using a centrifuge. It relates to a vegetable and marine collagen peptide production method comprising a.

Briefly describing the method for producing a collagen peptide according to the present invention.

In the present invention, in the production of collagen peptides, plants or seafood such as fruits, vegetables, seeds, nuts, mushrooms, seaweeds, fish and shellfish, and fish are used as raw materials.

First, a pretreatment step of pulverizing the raw material is performed to facilitate the enzyme action on the vegetable and seafood raw materials.

The organic acid immersion process is added to the raw material subjected to the pretreatment to prepare an organic acid immersion process having a pH which is an optimum activity condition of the enzyme, and an enzyme treatment step of adding and processing pepsin, which is a proteolytic enzyme, to the raw material solution.

The collagen peptide solution obtained by the enzyme treatment was first filtered to remove foreign substances (sludge), and then the solution was heated and subjected to enzymatic inactivation and sterilization, followed by secondary filtration using a centrifuge to complete the liquid collagen peptide. do.

In addition, it is possible to obtain a safe powder type collagen peptide by drying the liquid collagen peptide.

In the enzyme treatment step, it is possible to obtain a safe collagen peptide by enzymatic treatment at pH 7.5 to 8.5 at a temperature of 35 to 45 ° C. for 20 to 30 minutes using trypsin as a protease.

In addition, more preferably, by using papain as a protease, papain (Papain) by using an enzyme treatment at pH 5.0 ~ 9.0, temperature 40 ~ 60 ℃ for 30 to 40 minutes it can be obtained a safe excellent collagen peptide.

The protease is an enzyme for lowering the molecular weight of the raw collagen, it is preferable to use pepsin, trypsin, papain suitable for the characteristics of each raw material.

In addition, the enzyme used in the enzyme treatment step is preferably treated after adjusting the pH and temperature that can exhibit the optimum activity, pepsin is adjusted to pH 1.0 to 3.0, temperature is 25 to 40 ℃, Trypsin pH 7.5 to 8.5, the temperature is adjusted to 35 to 45 ℃, papain pH 5.0 to 9.0, the temperature is adjusted to 40 to 60 ℃ is a good condition for enzyme activation.

Hereinafter, a method for producing vegetable and marine collagen peptides having excellent safety of the present invention will be described in more detail at each step.

1. Raw material pretreatment step

The main objective is to increase the working area of the enzyme by removing foreign substances from the raw material and chopping finely to facilitate the action of the enzyme in subsequent processes.

Plants and seafood, which are raw materials of the present invention, are processed at room temperature or refrigerated, and then used to remove foreign substances and chop them into crushers.

According to the present invention, it is shown that depending on the degree of crushing the raw material, that is, the finer the crushing, the shorter the enzyme treatment time.

2. Dipping step of organic acid solution

As a process to easily enzymatically decompose the chopped raw materials, in order to exclude the use of inorganic acids having a strong toxicity above all, an organic acid solution having a pH of 2 to 3 is added at a ratio of 1: 2 to 1: 5 to the weight of the raw material, After making the state, keep the temperature at 30 ~ 50 ℃.

3. Enzyme Treatment Step

A process of hydrolyzing collagen in a molecular weight range of about 1,000 to 3,000 that is rapidly absorbed in the body by using a specific proteinase in an aqueous solution of an organic acid. Typically, a protein hydrolase is 0.1g to 1g in weight of an aqueous solution of an organic acid. Use for 2 hours to 24 hours. In particular, pepsin among proteolytic enzymes appears to be excellent.

Among the proteolytic enzymes, pepsin is a proteolytic enzyme that works in the stomach of the human body and preferentially hydrolyzes peptide bonds on the carboxyl side of Phenylalanine, leucine or Glutamic acid, and the protein at pH 1.0 ~ 3.0 and temperature 30 ~ 50 ℃ Since hydrolysis of is most preferred, it is preferable to treat the pH of the aqueous collagen peptide solution while adjusting the organic acid to an optimum condition.

The concentration results of the vegetable and marine collagen peptides prepared by the examples of the present invention are shown in Tables 1 and 2.

Changes in Concentration of Vegetable and Marine Collagen Collagen Peptides According to Enzyme Addition Conditions (Brix,%, Temperature 40 ℃) division 2 hours 6 hours 10 hours 14 hours 18 hours 22 hours 24 hours 0.1% addition 0.2 0.3 1.0. 1.6 1.8 1.8 1.8 0.3% addition 0.5 0.8 1.2 1.6 1.9 2.1 2.3 0.5% addition 1.5 2.0 2.3 2.5 2.7 2.8 3.0 0.7% addition 1.7 2.1 2.3 2.6 2.7 2.8 3.1 0.9% addition 2.0 2.4 2.5 2.6 2.8 2.9 3.1 1.0% addition 2.1 2.5 2.6 2.6 2.8 2.9 3.1

Changes in Concentration of Vegetable and Marine Collagen Peptides According to Temperature Conditions (Brix,%, Pepsin 0.5%) division 2 hours 6 hours 10 hours 14 hours 18 hours 22 hours 24 hours 30 ℃ 1.0 1.3 1.5. 1.6 1.8 2.0 2.1 35 ℃ 1.5 1.8 2.2 2.3 2.4 2.5 2.7 40 ℃ 1.5 2.0 2.3 2.5 2.7 2.8 3.0 45 ℃ 1.7 2.1 2.2 2.6 2.6 2.8 3.0 50 ℃ 1.9 2.3 2.4 2.6 2.7 2.9 3.1

4. First filtration step

When the concentration of the aqueous solution reaches the desired concentration, the collagen is extracted from the enzyme-treated raw material solution and subjected to the first filtration process to filter only the aqueous collagen solution by removing the sludge which is the remaining raw material sludge. The target concentration is then measured with a Brix meter.

5. Enzyme Inactivation and Sterilization

When the primary filtration step is completed, the temperature of the primary filtered aqueous solution is rapidly increased to 85 ° C. or higher to continue heating for a predetermined time to simultaneously disinfect the enzyme while sterilizing.

6. Second filtration step

In this step, the collagen peptide extract in which the enzyme is inactivated is removed using a centrifuge.

This secondary filtration step completes the vegetable and marine collagen liquid products.

7. Drying Step

The liquid product is dried using a drying apparatus such as a lyophilizer and a spray dryer to complete the vegetable and marine collagen peptide powder products.

Hereinafter, the configuration and the effect of the present invention through the embodiment will be described in more detail.

However, the following examples are merely for illustrative purposes and should not be construed as limiting the scope of the invention.

<Example 1>

After refrigeration, the raw material is chopped with a crusher, and after the raw material is pretreated, a citric acid solution having a pH of 2 to 3 is mixed with the raw material in a ratio of 1: 3 to prepare an aqueous solution. While adjusting the pH of the aqueous solution of the raw material to 3.0, 0.5% by weight of pepsin is added to the raw material and treated at 40 ° C. for 24 hours.

When the concentration of the enzymatically treated aqueous solution obtained 5% yield of collagen peptides compared to the raw material, the temperature was rapidly increased to 85 ° C. to terminate the reaction by disinfection and sterilization at the same time for 1 hour.

The sterilized collagen peptide extract is prepared from vegetable and marine collagen by removing foreign substances using a centrifuge.

<Example 2>

After chilled raw material is chopped with a crusher to pre-treat the raw material, an aqueous solution is prepared by mixing citric acid aqueous solution of pH 2-3 with a ratio of 1: 2. While adjusting the pH of the aqueous solution of the raw material to 2.0 g of pepsin by weight relative to the raw material is added and treated for 18 hours at 40 ℃.

When the concentration of the enzymatically treated aqueous solution is obtained 5% yield of collagen peptide compared to the raw material is rapidly raised to 85 ℃ to terminate the reaction by sterilization at the same time with the inactivation of the enzyme for 40 minutes.

The sterilized collagen peptide extract is prepared from vegetable and marine collagen by removing foreign substances using a centrifuge.

<Example 3>

After refrigeration, the raw material is chopped with a crusher, and then the raw material is pretreated, and an aqueous solution is prepared by mixing citric acid aqueous solution of pH 2-3 with a ratio of 1: 5. While adjusting the pH of the aqueous solution of the raw material to 2.0 g of pepsin compared to the raw material is added and treated at 40 ℃ for 10 hours.

When the concentration of the enzymatically treated aqueous solution is obtained 5% yield of collagen peptide compared to the raw material is rapidly raised to 85 ℃ to terminate the reaction by sterilization at the same time with the inactivation of the enzyme for 60 minutes.

The sterilized collagen peptide extract is prepared from vegetable and marine collagen by removing foreign substances using a centrifuge.

<Comparative Example 1>

The refrigerated raw material was chopped with a shredder and treated in a raw material treatment tank containing 1.1% aqueous sulfuric acid solution for 60 hours and then washed with about 10 times the washing water for 12 hours.

Water was added to the raw material subjected to the acid treatment and washing process and extracted three times at a temperature of 55 ° C., 65 ° C. and 80 ° C. with a hot bath to obtain raw collagen, followed by adding enzyme to the raw collagen for 2 hours. After the treatment, the resultant was filtered using a pulp filter and desalted and purified by passing a cationic and anionic mixture for 2 hours.

Thereafter, the desalted product is sterilized at high temperature to prepare water-soluble collagen.

<Comparative Example 2>

The refrigerated raw material was chopped with a crusher and treated for 48 hours in a raw material treatment tank containing 1.1% aqueous sulfuric acid solution, followed by washing for 10 hours with about 8 times the washing water of the raw material.

Water was added to the raw material subjected to the acid treatment and washing process and extracted three times at a temperature of 55 ° C., 65 ° C. and 80 ° C. with a hot bath to obtain raw collagen. The enzyme was added to the raw collagen for 2 hours. After treatment, the resultant is filtered using a pulp filter and desalted and purified by passing a cationic and anionic mixture for 2 hours.

Thereafter, the desalted product is sterilized at high temperature to prepare water-soluble collagen.

<Comparative Example 3>

The refrigerated raw material was chopped with a crusher and treated for 36 hours in a raw material treatment tank containing an aqueous 1.1% sulfuric acid solution and then washed for 8 hours with about 6 times the washing water of the raw material.

Water was added to the raw material subjected to the acid treatment and washing process and extracted three times at a temperature of 55 ° C., 65 ° C. and 80 ° C. with a hot bath to obtain raw collagen. The enzyme was added to the raw collagen for 2 hours. After treatment, the resultant is filtered using a pulp filter and desalted and purified by passing a cationic and anionic mixture for 2 hours.

Thereafter, the desalted product is sterilized at high temperature to prepare water-soluble collagen.

Experimental Example

In Examples 1 to 3 and Comparative Examples 1 to 3, the treatment time of each vegetable and marine collagen peptide was measured in the following process, and the results are shown in Table 3 below. The sensory test was carried out by the ranking method, and the results are shown in Table 4.

division Acid treatment time Cleaning time Extraction time Enzyme Processing Time Desalination Time Total Time Example 1 - - - 24 - 24 Example 2 - - - 18 - 18 Example 3 10 - 10 Comparative Example 1 60 12 18 2 2 94 Comparative Example 2 48 10 18 2 2 80 Comparative Example 3 36 8 18 2 2 66

As can be seen from the results of Table 3, Examples 1 to 3 vegetable and marine collagen peptides prepared by the production method of the present invention is compared with Comparative Examples 1 to 3 prepared by the conventional method of producing animal collagen By performing the enzyme treatment without going through the acid treatment, washing step, extraction step, after the desalination purification process also does not need to go through the enzyme treatment. Therefore, it can be seen that the total time is reduced by about 4.6 times.

Sensory Evaluation of Phyto- and Marine Collagen Peptides by Ranking Method Sensory tester One 2 3 4 5 6 7 8 9 10 Sum flavor Example 1 5 4 4 4 3 3 4 4 3 4 38 Example 2 5 5 4 3 4 4 4 4 4 4 41 Example 3 4 4 3 4 4 3 3 4 3 3 35 Comparative Example 1 2 3 2 2 3 2 2 2 One One 20 Comparative Example 2 2 2 3 3 3 2 2 One 3 3 24 Comparative Example 3 One 2 2 2 One 3 2 One 2 One 17 smell Example 1 3 3 5 4 4 3 4 4 4 4 38 Example 2 4 4 5 4 4 4 4 5 3 4 51 Example 3 4 3 4 3 4 4 3 4 4 4 37 Comparative Example 1 2 2 2 2 One One 2 One 2 One 16 Comparative Example 2 2 One One 2 2 2 2 2 2 2 18 Comparative Example 3 One 2 2 One 2 One One 2 2 One 15 color Example 1 5 4 4 4 5 3 4 3 3 4 39 Example 2 3 5 5 4 4 4 4 4 4 4 41 Example 3 4 4 4 4 4 3 4 4 3 3 37 Comparative Example 1 One 2 2 2 2 One One 2 One 2 16 Comparative Example 2 One 2 2 2 One 2 2 2 2 3 19 Comparative Example 3 2 2 One 2 One One 2 One 2 One 15 Overall preference Example 1 4 4 4 5 5 5 5 4 3 4 43 Example 2 5 5 4 5 5 4 4 5 4 4 45 Example 3 4 4 4 4 4 4 4 4 4 3 39 Comparative Example 1 One 2 One One 2 2 One One 2 2 15 Comparative Example 2 2 2 2 One 2 2 One 2 2 2 18 Comparative Example 3 2 One 2 2 2 One One 2 2 One 16

(Note) 1: Very bad 2: Bad 3: Normal 4: Good 5: Very good

The collagen peptide prepared according to the present invention can be substituted for animal collagen produced using animal raw materials as manufactured from vegetable or seafood.

In addition, the collagen peptide prepared by the present invention uses citric acid, which is an organic acid instead of a strong toxic acid, and does not undergo a chemical treatment or a desalination process, and thus does not have an inorganic acid or a heavy metal as an amino acid reinforcing agent of a functional beverage, health supplement or various processed foods. It is an excellent collagen peptide that can be easily used and used as a substitute for existing amino acids as a raw material of functional cosmetics.

In addition, as can be seen from the results of Table 4, the vegetable and marine collagen peptides prepared by the production method of the present invention compared to the collagen peptides prepared by the conventional production method of animal collagen, such as taste, aroma, smell Overall preference is appreciated.

In addition, in the present invention, by chopping the raw material finely in the pre-treatment step of the raw material to facilitate the action of the enzyme, it can increase the yield of the collagen produced.

In addition, in the present invention, since water is used as the solvent, there is an advantage that the environmental pollution problem and the wastewater treatment problem which are caused by the prior art do not occur at all.

While the invention has been described in detail only with respect to the described embodiments, it will be apparent to those skilled in the art that various modifications and variations are possible within the spirit of the invention, and such modifications and variations belong to the appended claims. .

Claims (5)

A raw material pretreatment step of removing and chopping the foreign materials of the raw material to form a raw material having a predetermined size; Immersing an organic acid solution in which an organic acid aqueous solution of the raw material is formed by adding an organic acid aqueous solution to the raw material; An enzyme treatment step of hydrolyzing the protein to form an aqueous collagen peptide solution by adding a specific proteolytic enzyme to the aqueous organic acid solution of the raw material; A first filtration step of forming a first filtered collagen peptide aqueous solution by removing sludge from the collagen peptide aqueous solution when collagen reaches a predetermined concentration in the collagen peptide aqueous solution; Enzymatic inactivation and sterilization step of heating the temperature of the primary filtration collagen peptide aqueous solution to a predetermined temperature or more, and deactivating and sterilizing the enzyme by maintaining the temperature for a specific time to form a sterilized collagen peptide aqueous solution. ; And A second filtration step of forming a second filtration collagen peptide aqueous solution by removing foreign matter from the sterilized collagen peptide aqueous solution using a centrifuge; Vegetable / marine collagen peptide manufacturing method comprising a. The method according to claim 1, wherein the raw material, Method for producing a vegetable / marine collagen peptide, characterized in that the plant or seafood. The proteinase of claim 1, wherein Trypsin, papain (Papain), pepsin (Pepsine) any one of the plant / marine collagen peptide manufacturing method characterized in that. The method of claim 1, A drying step of forming a powder collagen peptide by drying the second filtered collagen peptide aqueous solution using a drying apparatus; Vegetable / marine collagen peptide production method characterized in that it further comprises. Raw materials of plants or seafood, A raw material pretreatment step of forming a raw material of a predetermined size by removing and chopping foreign matters from the raw material; An organic acid solution immersion step of adding an organic acid aqueous solution to the chopped raw material to form an organic acid aqueous solution of the raw material; An enzyme treatment step of hydrolyzing the protein to form an aqueous collagen peptide solution by adding a specific proteolytic enzyme to the aqueous organic acid solution of the raw material; A first filtration step of forming a first filtered collagen peptide aqueous solution by removing sludge from the collagen peptide aqueous solution when collagen reaches a predetermined concentration in the collagen peptide aqueous solution; Enzymatic inactivation and sterilization step of heating the temperature of the primary filtration collagen peptide aqueous solution to a predetermined temperature or more, and deactivating and sterilizing the enzyme by maintaining the temperature for a specific time to form a sterilized collagen peptide aqueous solution. ; A second filtration step of forming a second filtration collagen peptide aqueous solution by removing foreign matter from the sterilized collagen peptide aqueous solution using a centrifuge; And A drying step of forming a powder collagen peptide by drying the second filtered collagen peptide aqueous solution using a drying apparatus; Vegetable / marine collagen peptides prepared by the production method comprising a.