KR100647033B1 - Process for preparing sterilized pure water-soluble collagen peptide - Google Patents
Process for preparing sterilized pure water-soluble collagen peptide Download PDFInfo
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- KR100647033B1 KR100647033B1 KR1020030060997A KR20030060997A KR100647033B1 KR 100647033 B1 KR100647033 B1 KR 100647033B1 KR 1020030060997 A KR1020030060997 A KR 1020030060997A KR 20030060997 A KR20030060997 A KR 20030060997A KR 100647033 B1 KR100647033 B1 KR 100647033B1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
Abstract
본 발명은 우피(Hide Splits), 돈피 (Pig Skin), 건조된 소뼈(Ossein), 어류 등을 원료로 추출조에 투입하는 공정 ; 상기 원료를 알칼리 처리, 산 처리, 알칼리 및 산 처리를 하는 세 단계의 전처리 공정 ; 추출 반응조에서 추출 후 여과·정제·농축·살균시켜 조 콜라겐 펩타이드를 추출하는 공정; 추출 용액에 단백질 분해효소 처리를 통해 저분자량화 시킨 후 재차 여과 및 살균·건조하는 공정을 포함하는 수용성 콜라겐 펩타이드의 제조방법을 제공하는 것이다.The present invention comprises the steps of adding (Hide Splits), pork skin (Pig Skin), dried bone (Ossein), fish, etc. as raw materials to the extraction tank; A three step pretreatment step of alkali treatment, acid treatment, alkali and acid treatment of the raw material; Extracting crude collagen peptide by filtration, purification, concentration, and sterilization after extraction in an extraction reactor; It is to provide a method for producing a water-soluble collagen peptide comprising the step of lowering the molecular weight through the proteolytic enzyme treatment to the extraction solution, and then again filtered and sterilized.
추출, 여과, 정제, 우피, 돈피, 건조된 소뼈, 어류, 듀얼소킹, 단백질 분해 효소, 수용성 콜라겐 펩타이드Extraction, Filtration, Purification, Bovine, Donpi, Dried Bone, Fish, Dual Soaking, Proteolytic Enzyme, Water Soluble Collagen Peptide
Description
본 발명은 우피, 돈피, 건조된 소뼈, 어류 등의 다양한 원료를 이용하여 천연 콜라겐 펩타이드를 추출, 분리시킨 후, 정제, 여과, 살균 공정을 통해 보다 다양한 특성을 지닌 멸균된 순수 수용성 콜라겐 펩타이드의 제조방법에 관한 것이다. The present invention is to extract and isolate the natural collagen peptide using a variety of raw materials such as cowhide, pork skin, dried bovine bone, fish, and then to produce a sterile pure water-soluble collagen peptide having more various characteristics through purification, filtration, sterilization process It is about a method.
더욱 상세하게는, 본 발명은 우피, 돈피, 건조된 소뼈, 어류에 이르기까지 다양한 원료를 이용하여 알칼리 처리, 산 처리, 알칼리 및 산으로 전처리 공정 후, 추출 반응조에서 추출단계를 거쳐 여과·정제·농축·살균시킨 조 콜라겐 펩타이드를 1차 효소처리 과정을 거쳐 저분자량화 시킨 후, 다시 한 번 정제·여과·살균 공정을 거침으로써 보다 우수하고 순수한 멸균 수용성 콜라겐 펩타이드의 제조방법 에 관한 것이다.More specifically, the present invention is filtered, purified and purified through an extraction step in an extraction reactor after alkali treatment, acid treatment, pretreatment with alkali and acid using various raw materials such as cow skin, pork skin, dried bovine bone and fish. The present invention relates to a method for producing superior and pure sterile water-soluble collagen peptides by lowering the molecular weight of the concentrated and sterilized crude collagen peptide through the first enzymatic treatment and then subjecting the purification, filtration and sterilization process.
콜라겐 펩타이드는 프롤린, 옥시 프롤린, 글리신, 글루타민산등으로 구성된 섬유성 단백질의 일종으로 약 1000개의 아미노산이 모여 가늘고 긴 띠의 형상을 지닌 단백질이다. 사람의 몸에서 특히 장기를 감싸는 막, 관절 연골, 눈의 각막, 뼈와 피부 등에 주로 존재하고, 특히 피부 속 진피층의 구성 성분으로 매우 중요한 역할을 하고 있다.Collagen peptide is a kind of fibrous protein composed of proline, oxyproline, glycine, glutamic acid, etc., and has a long strip of protein with about 1000 amino acids. In the human body, especially the organs surrounding the membranes, articular cartilage, cornea of the eye, bones and skin, etc. are mainly present, and play a very important role as a component of the dermal layer in the skin.
콜라겐 펩타이드는 건강식품, 외과 또는 안과용 치료제 또는 화장품용 첨가제로 사용되고 있으며, 이러한 건강식품, 의학적 용도로 사용하기 위해서는 천연 상태에서 추출된 콜라겐 펩타이드를 완벽하게 멸균시켜야 하는 필요성이 제기된다.Collagen peptides are used as additives for health foods, surgical or ophthalmic therapies or cosmetics, and the use of such health foods and medical uses raises the need to completely sterilize collagen peptides extracted from a natural state.
종래의 콜라겐 펩타이드 제조방법은 돈피(Pig Skin)를 전처리 단계에서 산 처리를 행한 다음, 추출단계, 추출물에 효소처리를 하여 수용성 콜라겐 제조 단계 및 정제 단계로 구성되어 있거나, 돈피를 산 처리하지 않고 쵸핑한 다음 효소를 1, 2차에 걸쳐 투입하여 수용성 콜라겐 펩타이드를 제조하였다. 하지만 이 방법은 콜라겐의 순도가 떨어지고 회분이 증가되었다.Conventional collagen peptide production method is an acid treatment of pig skin (Pig Skin) in the pre-treatment step, followed by the extraction step, the enzyme treatment of the extract is composed of a water-soluble collagen production step and purification step, or chopping the pig skin without acid treatment Then, the enzyme was added to the first and second stages to prepare a water-soluble collagen peptide. However, this method reduced the purity of collagen and increased the ash content.
따라서 순도의 증가와 회분을 감소시켜 보다 우수하고 순수한 수용성 콜라겐 펩타이드를 제조하여야 하는 문제와 수용성 콜라겐 펩타이드 제조 원료를 돈피만 사용함으로써 원료 수급이 원활하지 못하고 또한 돈피에 대해 혐오감을 느끼는 고객이 많은 이유로 인해 돈피를 이용한 수용성 콜라겐 발전에 애로사항이 많아 돈피를 대체할 수 있는 원료 개발이 시급한 문제로 대두되었다.Therefore, due to the increase in purity and the reduction of ash, it is necessary to manufacture better and pureer water-soluble collagen peptides, and the reason why many customers who are dissatisfied with raw material supply and are disgusted with pork skin by using only raw blood Due to the difficulties in developing water-soluble collagen using pork skin, the development of raw materials to replace pork skin has emerged as an urgent problem.
이에, 본 발명이 이루고자 하는 기술적 과제는 우피(Hide Splits), 돈피 (Pig Skin), 건조된 소뼈(Ossein), 어류를 원료로 하여 원료 수급을 해결하고 나아가 원료처리 기술의 개발과 정제 및 여과공정에 변화를 주어 보다 우수하고 순수한 수용성 콜라겐 펩타이드를 개발함으로써 고객의 요구 조건을 충족하는데 그 목적이 있다.
Therefore, the technical problem to be achieved by the present invention is to solve the supply and demand of raw materials (Hide Splits), pork skin (Pig Skin), dried beef bones (Ossein), fish as raw materials, further development of raw material processing technology, purification and filtration process The goal is to meet the customer's requirements by developing a better, more pure, water-soluble collagen peptide.
본 발명의 목적은 우피(Hide Splits), 돈피 (Pig Skin), 건조된 소뼈(Ossein), 어류 등을 원료로 추출조에 투입하는 공정 ; 상기 원료를 알칼리 처리, 산 처리, 알칼리 및 산 처리를 하는 세 단계의 전처리 공정 ; 추출 반응조에서 추출 후 여과·정제·농축·살균시켜 조 콜라겐 펩타이드를 추출하는 공정; 추출 용액에 단백질 분해효소 처리를 통해 저분자량화 시킨 후 재차 여과 및 살균·건조하는 공정을 포함하는 수용성 콜라겐 펩타이드의 제조방법을 제공하는 것이다.An object of the present invention is a step of putting Hide Splits, Pig Skin, dried bones (Ossein), fish and the like into the extraction tank as raw materials; A three step pretreatment step of alkali treatment, acid treatment, alkali and acid treatment of the raw material; Extracting crude collagen peptide by filtration, purification, concentration, and sterilization after extraction in an extraction reactor; It is to provide a method for producing a water-soluble collagen peptide comprising the step of lowering the molecular weight through the proteolytic enzyme treatment to the extraction solution, and then again filtered and sterilized.
이 때 상기 원료처리 공정에 있어서 알칼리 처리 시간은 24∼48시간이고 pH는 13.0∼13.6이며, 산 처리 시간은 18∼28시간이고 pH는 1.5∼2.0 이다. 또한 효소 처리 공정에 있어서는 상기 콜라겐 펩타이드에 대하여 단백질 분해 효소 0.1∼0.2wt% 만을 투입하여 50 내지 55℃에서 1시간 동안 처리하여 보다 저분자화 된 수용성 콜라겐 펩타이드를 제조한 후 카본 필터 시스템 및 살균 공정을 거쳐 스프레이 드라이어로 분말화 시킨다.At this time, in the raw material treatment step, the alkali treatment time is 24 to 48 hours, the pH is 13.0 to 13.6, the acid treatment time is 18 to 28 hours, and the pH is 1.5 to 2.0. In addition, in the enzyme treatment step, only 0.1-0.2 wt% of the protease is added to the collagen peptide, and then treated at 50 to 55 ° C. for 1 hour to produce a lower molecular weight water-soluble collagen peptide, followed by a carbon filter system and sterilization process. Powdered by spray dryer.
상기 효소 처리 공정에 있어서 단백질 분해 효소는 바실러스 서브틸러스 (Bacillus subtilis)를 사용하였으며, 효소 및 콜라겐에서 나오는 극미세 입자 및 이취를 없애기 위해서 산화제를 쓰지 않고 한 번 더 카본 필터 시스템을 통과시킴으로써 순수한 콜라겐을 제조할 수 있으며 용액의 살균을 위해서 142℃ 이상에서 6∼7초간 살균공정을 거친 후 스프레이 드라이어를 이용하여 고온(175∼200℃), 고압(80∼90bar)으로 분사시켜 용액을 분말로 형성시킨다. 여기서 입자도는 분말 형성과정에서 조절할 수 있다. (50∼150㎛)In the enzyme treatment process, Bacillus subtilis was used as a proteolytic enzyme, and pure collagen was passed through a carbon filter system once more without using an oxidant to remove micro particles and odors from enzymes and collagen. After sterilizing for 6-7 seconds at 142 ℃ or higher for sterilization of the solution, the solution is formed into powder by spraying at high temperature (175-200 ℃) and high pressure (80-90bar) using a spray dryer. Let's do it. The particle size can be adjusted during the powder formation process. (50 to 150 μm)
또한 상기 효소 처리 공정에 있어서 단백질 분해 효소가 활동하기에 가장 적합한 조건을 만들어 주는 것이 바람직하며, pH는 5.0∼6.5이고 온도는 50∼55℃이다.In addition, it is preferable to make the most suitable conditions for the proteolytic enzyme to act in the enzyme treatment process, the pH is 5.0 to 6.5 and the temperature is 50 to 55 ℃.
이하 본 발명을 더욱 상세히 설명한다.Hereinafter, the present invention will be described in more detail.
따라서 본 발명은 돈피뿐만 아니라 우피(Hide), 건조된 소뼈(Ossein), 어류를 사용하여 원료 수급 문제를 해결하였고 또한 돈피에 대해 혐오감을 느끼는 고객을 위하여 또 다른 수용성 콜라겐 펩타이드를 공급함으로써 수용성 콜라겐 펩타이드의 수요를 증가시킬 수 있었다.Therefore, the present invention solves the raw material supply and demand problem using hides, dried beef bones, fish as well as pork skin and also supplies water-soluble collagen peptides for customers who feel disgusted with pork skin. Could increase demand.
그리고 산 처리 기술 이외도 알칼리 처리, 알칼리 처리와 산 처리 겸한 듀얼소킹(Dual Soaking)을 개발함으로써 다양한 특성을 가진 수용성 콜라겐 펩타이드를 만들 수가 있었으며 여러 번의 정제·여과·살균을 거침으로써 보다 우수하고 순수한 수용성 콜라겐 펩타이드를 만들 수 있었다.In addition to the acid treatment technology, dual soaking combined with alkali treatment, alkali treatment and acid treatment was able to produce water-soluble collagen peptides with various characteristics.As a result of several purification, filtration and sterilization, superior and pure water solubility was achieved. Collagen peptides could be made.
이와 같은 본 발명은 실시예에 의거하여 상세하게 설명한다. 그러나 본 발명의 범위가 하기 실시예에 의해 한정되는 것은 아니다.This invention is demonstrated in detail based on an Example. However, the scope of the present invention is not limited by the following examples.
(실시예 1)(Example 1)
정제수 10L에 수산화나트륨용액(순도 33%)을 443㎖을 넣어서 pH 13.4의 알칼리 수용액을 제조한다. 여기에 수분함량 14%인 건조된 어류 비늘을 교반기를 계속 가동시키면서 1㎏ 투입한 후 교반기를 계속 가동시키면서 36시간 지속시킨다. 이러한 알칼리 처리가 종료된 후 알칼리수는 완전히 드레인 시킨 후 10L씩의 정제수로 약 30분씩 6회 세척을 실시하여 pH10.0∼11.0 범위로 조정한다.443 ml of sodium hydroxide solution (purity 33%) was added to 10 L of purified water to prepare an alkaline aqueous solution having a pH of 13.4. Here, 1 kg of dried fish scales having a water content of 14% were added while keeping the stirrer running, and the stirrer was continued for 36 hours. After the completion of the alkali treatment, the alkaline water is completely drained and washed six times with 10 L of purified water for about 30 minutes to adjust the pH to 10.0 to 11.0.
알칼리 처리가 끝난 어류비늘 1㎏이 담긴 pH 10이하로 된 10L물에 염산 용액(순도35%) 530㎖을 넣어서 pH1.8로 하여 교반기를 계속 가동시키면서 24시간 지속시킨다. 이러한 산 처리가 종료된 후 산 처리수를 완전히 드레인 시킨 다음 10L씩의 정제수로 약 30분씩 5회 세척을 실시하여 pH 4.5∼5.5 범위로 맞춘다.530 ml of hydrochloric acid solution (35% purity) was added to 10 L of water having a pH of 10 or less containing 1 kg of alkali-treated fish scales, and the pH was maintained at pH 1.8, and the stirrer was continued for 24 hours. After the acid treatment is completed, the acid treated water is completely drained, and then washed five times with about 10 minutes each of purified water at 10 L to adjust the pH to 4.5 to 5.5.
원료의 전처리가 완료된 이러한 어류비늘을 50℃ 정제수 3L를 담은 추출용기에 넣는다. 추출용기 내의 용액의 pH는 5.0∼6.5범위를 유지하고, 추출용기 바닥에 설치된 스팀코일로 스팀을 공급하면서 용액을 펌프를 이용 자체 순환시킴으로써 용액의 온도를 55∼60℃로 유지시킨다. 추출시간을 8시간으로 추출된 용액의 젤라틴 농도는 6%정도이며, 195g 상당의 젤라틴을 함유한다.The fish scales after the pretreatment of the raw material is completed are placed in an extraction container containing 3 liters of purified water 50 ℃. The pH of the solution in the extraction vessel is maintained in the range of 5.0 to 6.5, and the temperature of the solution is maintained at 55 to 60 ℃ by supplying steam to the steam coil installed at the bottom of the extraction vessel by circulating the solution by a pump. The extraction time is 8 hours, the gelatin concentration of the extracted solution is about 6%, and contains 195g of gelatin.
추출된 용액을 1차 여과를 실시하여 깨끗한 액을 이온교환수지를 통과시켜 미네랄을 제거하며, 이온교환수지를 통과한 용액의 pH는 5.0∼6.5 범위를 유지한다.The filtered solution was subjected to primary filtration to remove minerals by passing the clean liquid through the ion exchange resin, and the pH of the solution passed through the ion exchange resin was maintained in the range of 5.0 to 6.5.
이온교환수지를 통과한 용액을 2단계 멤브레인을 통과시켜 6%용액을 12%까지 자연 농축시킨 다음 2차 여과를 실시한다.The solution passed through the ion exchange resin was passed through a two-stage membrane to naturally concentrate the 6% solution to 12%, followed by secondary filtration.
2차 여과까지 완료된 용액을 진공 하에서 농축하여 농도 30∼35%로 유지시킨 다음 3차 카본 필터를 실시함으로써 완전히 정제된 용액 조건을 갖추도록 한다.The solution, which was completed by secondary filtration, was concentrated under vacuum to maintain a concentration of 30-35% and then subjected to a tertiary carbon filter to ensure fully purified solution conditions.
여과·정제·농축·살균된 추출 용액에 단백질 분해 효소 0.2㎖를 첨가하고 50∼55℃에서 1시간 처리하여 저분자량화 시킨 후 다시 정제·여과·살균을 거친다.0.2 ml of protease is added to the filtered, purified, concentrated, and sterilized extract solution, treated at 50 to 55 ° C for 1 hour to lower the molecular weight, and then purified, filtered, and sterilized.
최종적으로 스프레이 드라이어로 분말화 시켜 185g의 수용성 콜라겐 펩타이드를 얻었다. 수득된 수용성 콜라겐 펩타이드의 색상은 매우 엷은 담황색이었고, 회분 함량은 0.8중량%이하였으며, 수분 함량은 5∼8중량%이었다. 점도는 18mPs이하이었다. Finally powdered with a spray dryer to obtain a water-soluble collagen peptide of 185g. The color of the obtained water-soluble collagen peptide was very pale yellow, ash content was less than 0.8% by weight, and water content was 5-8% by weight. The viscosity was 18 mPs or less.
(실시예 2)(Example 2)
건조된 소뼈 3.0㎏를 사용하여 실시예 1에서와 같이 처리한 결과 530g의 수용성 콜라겐 펩타이드를 얻었다. 수득된 수용성 콜라겐 펩타이드의 색상은 매우 엷은 담황색이었고, 회분 함량은 1.0중량%이하였으며, 수분 함량은 6∼8중량%이었다. 점도는 25mPs이하이었다. 3.0 kg of dried bovine bone was treated as in Example 1 to obtain 530 g of water-soluble collagen peptide. The color of the obtained water-soluble collagen peptide was very pale yellow, ash content was less than 1.0% by weight, and water content was 6-8% by weight. The viscosity was 25 mPs or less.
(실시예 3)(Example 3)
우피(Hide Splits) 5㎏를 사용하여 실시예 1에서와 같이 처리한 결과 592g 의 수용성 콜라겐 펩타이드를 얻었다. 수득된 수용성 콜라겐 펩타이드의 색상은 매우 엷은 담황색이었고, 회분 함량은 1.2중량%이하였으며, 수분 함량은 5∼7중량%이었다. 점도는 23mPs이하이었다. 5 kg of hide splits were used as in Example 1 to obtain 592 g of water-soluble collagen peptide. The color of the obtained water-soluble collagen peptide was very pale yellow, ash content was less than 1.2% by weight, and water content was 5-7% by weight. The viscosity was 23 mPs or less.
(실시예 4)(Example 4)
돈피(Pig Skin) 5㎏를 사용하여 실시예 1에서와 같이 처리한 결과 653g의 수용성 콜라겐을 얻었다. 수득된 수용성 콜라겐 펩타이드의 색상은 매우 엷은 담황색이었고, 회분 함량은 0.8중량%이하였으며, 수분 함량은 5∼7중량%이었다. 점도는 20mPs이하이었다. 6 kg of water-soluble collagen was obtained as a result of treatment as in Example 1 using 5 kg of pig skin. The color of the obtained water-soluble collagen peptide was very pale yellow, ash content was less than 0.8% by weight, and water content was 5-7% by weight. The viscosity was 20 mPs or less.
(비교예)(Comparative Example)
비교예에 사용된 제조방법은 통상의 콜라겐 펩타이드 제조방법을 통한 것이다. 원료로써 돈피를 사용하였으며, 전처리 공정은 산 처리 또는 쵸핑을 실시하였다. 효소의 투입도 추출 반응조에서 1차 추출후 또는 쵸핑후 투입하였으며 효소의 처리시간은 2∼6시간이었다. 여과, 정제, 살균의 횟수는 통상 1회였다. The preparation method used in the comparative example is through a conventional collagen peptide preparation method. Pork skin was used as a raw material, and the pretreatment process was subjected to acid treatment or chopping. The enzyme was also added after extraction or chopping in the extraction reactor, and the enzyme treatment time was 2 to 6 hours. The number of times of filtration, purification and sterilization was once.
비교예의 방법을 통해 얻어진 수용성 콜라겐 펩타이드는 담황색을 띄고 수분함량은 10중량%이하이었고, 회분 함량은 2.5중량% 이하이었다. The water-soluble collagen peptide obtained through the method of Comparative Example was pale yellow, the water content was 10% by weight or less, and the ash content was 2.5% by weight or less.
실시예와 비교예의 제조방법상의 차이를 표1에 나타내었다. Table 1 shows the differences between the preparation methods of the examples and the comparative examples.
실시예와 비교예를 통해 제조된 제품의 성상의 차이를 표2에 나타내었다. Table 2 shows the differences in the properties of the products produced through the Examples and Comparative Examples.
본 발명의 효과는 우피(Hide Splits), 돈피 (Pig Skin), 건조된 소뼈(Ossein),어류 등 다양한 원료를 사용하여 원료 수급 문제를 해결하였고 또한 돈피에 대해 혐오감을 느끼는 고객을 위하여 또 다른 수용성 콜라겐 펩타이드를 제공함으로써 수요의 증가를 가져왔다. 한편, 원료처리 기술 개발로 다양한 특성의 수용성 콜라겐 펩타이드를 제조하였고, 여과·정제·농축·살균된 추출 용액에 한 번의 효소 투입으로 효소 처리시간 단축 및 효소 투입량의 절감을 가져왔으며, 정제·여과·살균의 공정을 다시 거침으로써 회분이 낮고 순도가 높은 순수한 수용성 콜라겐 펩타이드를 제조할 수 있게 되었다.
The effects of the present invention solve the problem of supply and demand using a variety of raw materials, such as hides (pig skin), pig skin (pig skin), dried bone (ossein), fish, and also another water-soluble for customers who feel aversion to pork skin Providing collagen peptides has led to an increase in demand. On the other hand, we developed water-soluble collagen peptides with various characteristics through the development of raw material processing technology, and reduced enzymatic processing time and enzymatic input amount by one enzymatic injection into filtered, purified, concentrated and sterilized extract solution. The sterilization process again allows for the production of pure water-soluble collagen peptides with low ash content and high purity.
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| KR100837858B1 (en) * | 2006-12-18 | 2008-06-13 | 한국식품연구원 | Preparation of soluble oligopeptide from pork-skin using irradiation |
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| KR101892939B1 (en) * | 2017-06-01 | 2018-08-29 | 김봉기 | Amino acid fertilizer composition treated by acid, alkali and protease from livestock byproduct |
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| KR102259842B1 (en) * | 2019-07-23 | 2021-06-01 | 충북대학교 산학협력단 | Manufacturing method of low molecular collagen peptide using natural proteinase |
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| KR101980361B1 (en) * | 2016-02-05 | 2019-08-28 | 아미코젠주식회사 | Collagen hydrolysate having high concentration of collagen tripeptide and uses thereof |
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