KR20200090628A - Hibiscus extracts containing food composition thereof and preparation method of the hibiscus extracts - Google Patents

Abstract

The present invention relates to a food composition containing a hibiscus extract containing vegetable collagen prepared by a more simplified process and a method for producing a hibiscus extract. The present invention includes a pre-treatment step, an alcohol extraction step, a hot water extraction step, and a separation and purification step.

Description

Food composition containing hibiscus extract and manufacturing method of hibiscus extract {HIBISCUS EXTRACTS CONTAINING FOOD COMPOSITION THEREOF AND PREPARATION METHOD OF THE HIBISCUS EXTRACTS}

The present invention relates to a food composition containing a hibiscus extract containing vegetable collagen prepared in a more simplified process and a method for preparing a hibiscus extract.

Collagen is a protein fiber in which three polypeptides are twisted in a fibrous form as one of the main components of animal skin or organs.

The general collagen manufacturing method is manufactured through a complicated manufacturing process including chemical or heavy metal treatment or by an enzymatic treatment process. Animal collagen is avoided by diseases such as mad cow disease and foot and mouth disease, and marine collagen is also marine pollution. Therefore, the development of alternative products is required as a situation of concern, and the development of a manufacturing method that can be safely applied as a raw material for food and simplify the manufacturing process is also required.

Domestic Publication No. 10-2004-0027283, Plant and Marine Collagen Peptide Manufacturing Method Domestic Publication No. 10-2015-0085565, Young collagen dry powder containing edible plant extract and functional flour prepared by mixing it

It is an object of the present invention to provide a food composition containing a hibiscus extract comprising vegetable collagen produced in a more simplified process and a method for preparing the hibiscus extract.

In order to achieve the above object, the food composition according to an embodiment of the present invention contains a hibiscus extract and cyclodextrin comprising vegetable collagen.

In order to achieve the above object, a method for preparing a hibiscus extract according to another embodiment of the present invention includes a pre-treatment step of preparing dried hibiscus fruits by grinding; An alcohol extraction step of preparing an alcohol extract by immersing the hibiscus fruit in an alcohol solvent; A hot water extraction step of mixing the alcohol extract and water and heating to prepare a hot water extract; And a separation and purification step of preparing the hibiscus extract containing vegetable collagen by purifying the hot water extract using a centrifuge.

The food composition containing the hibiscus extract of the present invention and the method for preparing the hibiscus extract can extract vegetable collagen in a purified process, and can solve the concerns of animal collagen or marine collagen and serve as a substitute. In addition, ethanol extraction and hot water extraction and filtration after separation and purification using a centrifuge, chemical treatment is excluded and the process can be safely used as vegetable raw material to simplify the process so that vegetable collagen can be used to prepare a food composition containing hibiscus extract have.

1 is a flow chart showing a method for extracting vegetable collagen from hibiscus fruit according to the manufacturing method of the present specification.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art to which the present invention pertains can easily practice. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein.

Hereinafter, the present invention will be described in more detail.

In order to achieve the above object, the food composition according to an embodiment of the present invention contains a hibiscus extract and cyclodextrin comprising vegetable collagen.

Hibiscus (Hibiscus) is a generic name for a plant belonging to the dicotyledonous mallow family and hibiscus genus. Leaves are alternate, 9cm long, with petiole, wide egg-shaped, pointed tip, irregular serrate. The leaf surface is glossy and dark green. Flowers are broad funnel-shaped, hang one on the axilla at the top of the new branch, and have small flower branches. The calyx is shaped like a barrel, and the end is divided into five. The petals are red and five, and the stamens are combined like a barrel, and a lot of anthers hang at the end. It blooms from summer to autumn, but blooms year-round when the proper temperature is maintained. In winter, flowering stops due to low temperature, grows well at 16℃ to 30℃, overwinters at 5℃ and likes light. More than 3,000 species have been developed in the state of Hawaii in the United States, and the colors of the flowers are white, red, magenta, red, yellow, etc., and there are also double flowers, and some flowers have a diameter of 10 cm to 25 cm. In addition, the hibiscus is known to have an effective physiologically active substance in the human body, which quickly excretes waste products during drinking, and is effective in atherosclerosis, anti-cancer components, enteritis, and bronchitis. In addition, pyruvic acid (pyruvic acid) in hibiscus sabrifera extract is known to have a protein-lowering effect as well as a weight-reducing effect by promoting lipolysis when ingested with dihydroxyacetone (DHA).

In the present invention, a hibiscus fruit is applied to obtain a vegetable extract, which is applied to a food composition.

Specifically, the hibiscus extract may be a hibiscus fruit extract.

Specifically, the hibiscus extract may be a hibiscus fruit ethanol extract.

Specifically, the hibiscus extract may be a hot water extract of hibiscus fruit ethanol extract.

Specifically, the hibiscus extract may include vegetable collagen separated from the hot water extract of the hibiscus fruit ethanol extract.

The specific method of manufacturing the hibiscus extract is redundant with the description of the manufacturing method to be described later, so the description thereof is omitted.

The food composition contains cyclodextrin.

The cyclodextrin is a white crystalline or crystalline powder that is odorless and has a slight sweetness as a food additive. For example, it can be produced by acting a transferase (Cycloamylose glucano transferase: CGT-Ase) to produce starch Bacillus macerans There are 6 to 12 glucoses, each of which forms a ring-shaped oligosaccharide having α-1,4 glycosidic bonds. According to its form, it is classified as α-cyclodextrin, β-cyclodextrin, and γ-cyclodextrin.

In the present invention, the cyclodextrin is added to increase the tackiness and viscosity of food, enhance the emulsification stability, and improve the properties and feel of food, and is applied as a binding agent to stabilize the hibiscus extract of the present invention containing vegetable collagen .

The hibiscus extract and the cyclodextrin may be included in a weight ratio of 1: 0.7 to 1.5, and may include 1: 0.9 to 1.1 weight fertilizer. When the hibiscus extract and the cyclodextrin are applied together to the food composition in such a content, when the prepared food composition is powdered while promoting sufficient stabilization of the hibiscus extract, it can provide excellent touch and physical properties.

The food composition may further include a carrier, a diluent, and the like applicable as a food composition in addition to the cyclodextrin described above. For example, lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methylcellulose, microcrystalline cellulose, polyvinyl Pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, and mineral oil.

The food composition may have a dry powder form. The dry powder may be, for example, lyophilized powder or hot air dried powder. When the food composition is stored in the form of a dry powder, it is possible to increase storage ease and storage efficiency, and is convenient to apply to itself or to various types of food compositions described later.

The food composition may be prepared in various forms according to a conventional method. For example, as a health food, the hibiscus extract itself may be prepared in the form of tea, juice, and drink to be liquefied, granulated, encapsulated, and powdered for drinking. In addition, the hibiscus extract can be prepared in the form of a composition by mixing with a known active ingredient known to have a skin-beauty effect. In addition, functional foods include, but are not limited to, beverages (including alcoholic beverages), fruits and processed foods thereof (e.g. canned fruits, canned fruits, jams, marmalades, etc.), fish, meat, and processed foods thereof (e.g. ham , Sausage corn beef, etc.), breads and noodles (e.g. udon, soba noodles, ramen, spagate, macaroni, etc.), juice, various drinks, cookies, syrup, dairy products (e.g. butter, cheese, etc.), edible plant maintenance , Margarine, vegetable protein, retort food, frozen food, various seasonings (eg, miso, soy sauce, sauce, etc.) can be prepared by adding the hibiscus extract. The preferred content of the hibiscus extract in the food composition is not limited to this, but is preferably from 0.01 to 50% by weight of the finally prepared food.

In addition, the food composition may further include other additives as necessary, for example, flavor enhancers, colorants, natural flavors, natural pigments, etc. may be included in the additives.

A method for preparing a hibiscus extract according to another embodiment of the present invention includes a pre-treatment step; Alcohol extraction step; Hot water extraction step; And separation and purification step; including the hibiscus extract containing vegetable collagen is prepared.

The pre-treatment step is a step of preparing the dried hibiscus fruit by grinding. The hibiscus fruit can be applied by crushing the washed and/or dried ones. In particular, the hibiscus fruit may be prepared by grinding its size to be about 30 to 150 mesh, specifically about 50 to 100 mesh. The pulverization may be applied to the pulverization, which is usually applied, and the method or application device is not limited.

The alcohol extraction step is a step of preparing an alcohol extract by immersing the hibiscus fruit in an alcohol solvent.

The alcohol solvent is a solvent containing alcohol, the alcohol may be specifically alcohol or alcohol having 1 to 5 carbon atoms, may be a mixture of the alcohol and hexane, chloroform, or water. Specifically, the alcohol solvent may include i) methanol, ethanol, alcohol, and mixtures thereof and ii) water. More specifically, the alcohol solvent may be an ethanol solvent containing 50 to 90% by weight of anhydrous ethanol. When the alcohol solvent is applied to the above manufacturing method, useful components such as collagen can be efficiently extracted from the hibiscus fruit.

In the alcohol extraction step, the crushed hibiscus fruit and the alcohol solvent may be applied in a weight ratio of 1: 2 to 15, and may be applied in a weight ratio of 1: 5 to 11. When applied at this rate, a more efficient extraction process can be performed.

The alcohol extraction step may be performed at room temperature (15 to 28 ℃). Specifically, the alcohol extraction step may be performed by immersing and standing at room temperature. The alcohol extraction step may be performed for 10 to 40 hours, and may be performed for 18 to 30 hours. When the alcohol extraction step is applied for less than 10 hours, it may be difficult to sufficiently extract components such as collagen, and when applied for more than 40 hours, the efficiency of extraction may be unnecessarily impaired.

The alcohol extract thus obtained is a mixture of the crushed hibiscus fruit and the alcohol extract and can be applied to the hot water extraction step without filtration.

The hot water extraction step is a step of mixing the alcohol extract and water and heating to prepare a hot water extract.

The water may be purified water, deionized water, or the like, and there is no particular limitation on the type of water.

In the hot water extraction step, the alcohol extract and the water may be applied in a weight ratio of 1: 1 to 12, and may be applied in a weight ratio of 1: 3 to 8. When applied at this rate, a more efficient extraction process can be performed.

로 진행될 수 있고, 상기 가온은 75 내지 85 ℃로 진행될 수 있다. 이러한 온도의 범위로 가온이 진행되는 경우, 열수추출과정이 보다 효율적으로 적용될 수 있으며, 콜라겐이 보다 효율적으로 상기 알코올추출물 등으로부터 열수로 추출해낼 수 있다.The heating in the hot water extraction step is 60 to 98

It may proceed to, and the heating may proceed to 75 to 85 ℃. When the heating is performed in a range of such a temperature, the hot water extraction process can be applied more efficiently, and collagen can be more efficiently extracted with hot water from the alcohol extract or the like.

The hot water extraction step may be performed for 10 to 72 hours, and may be performed for 16 to 30 hours. When the hot water extraction step is applied for less than 10 hours, components such as collagen may not be sufficiently extracted, and when it is performed for more than 72 hours, the efficiency of extraction may decrease.

The hot water extract obtained in the hot water extraction step is removed from the solid in the subsequent process and separated/purified to prepare a hibiscus extract containing collagen.

The separation and purification step is a step of preparing a hibiscus extract containing vegetable collagen by purifying the hot water extract using a centrifuge.

In the separation and purification step, the filtration process, the first separation and purification process, and the second separation and purification process may be sequentially performed.

Specifically, the filtration process is a process of preparing a liquid extract by removing the solid material from the hot water extract. The removal of the solid material can be applied without limitation, as long as it is a method for removing the solid material from the extract, and a filtration method using an exemplary filter can be applied. For example, the filter may have a filter hole of 0.3 to 0.7 um.

The primary separation and purification process is a process of preparing the primary purification by separating the liquid extract in a one-dimensional depth at a gravitational acceleration of 2,000 G or less.

The gravitational acceleration applied in the first separation and purification process may be 2,000 G or less, 1,500 G or less, or 1,300 G or less. In addition, the gravitational acceleration applied in the first separation purification process may be 500 G or more, and may be 800 G or more.

In the first separation and purification process, the one-dimensional seam separation may be performed for 1 to 30 minutes, and may be performed for 5 to 20 minutes. When the gravitational acceleration of the primary separation and purification process is applied in this range, an efficient centrifugation process can be applied.

The second separation and purification process is a process of preparing a hibiscus extract, which is a second purification, by separating the first purification in a two-dimensional seam at a gravitational acceleration of 10,000 G or more.

The gravitational acceleration of the second separation and purification process may be 10,000 G or more, 30,000 G or more, and 70,000 G or more. In addition, the gravitational acceleration of the second separation and purification process may be 100,000 G or less, 90,000 G or less, and 85,000 G or less. In the second separation and purification process, the two-dimensional seam separation may be performed for 30 to 180 minutes, and may be performed for 30 to 90 minutes. When centrifugation is applied for a relatively long time by applying such a strong gravitational acceleration, vegetable collagen can be purified and separated through a centrifugation process.

The gravitational acceleration applied to the one-dimensional seam separation and the two-dimensional seam separation is preferably applied to a difference of 1: 30 to 100 times, and preferably applied to a difference of 1: 60 to 90 times, 1: 75 It is good to apply to make a difference of 85 to 85 times. In this way, when applying 1-dimensional and 2-dimensional separation, a hibiscus extract containing collagen can be obtained from the liquid extract more efficiently.

In this way, the hibiscus extract containing collagen obtained from the two-dimensional deep process can be obtained by separating each layer of the extract divided into a plurality of layers in a liquid phase and confirming a layer containing collagen. The method of confirming whether the collagen is contained may be applied without limitation as long as it is a method of confirming and detecting whether the collagen is contained.

The obtained hibiscus extract containing vegetable collagen may be prepared as a hibiscus extract in powder form after drying. At this time, the cyclodextrin, which acts as a stabilizer or binder, may be mixed into a proportion of the content described above and then dried and powdered. Hot air drying or final drying may be applied as the drying method.

Hereinafter, the present invention will be described in more detail through specific examples. The following examples are only examples for helping the understanding of the present invention, and the scope of the present invention is not limited thereto.

1. Pre-treatment step

For smooth extraction of the ingredients, the dried hibiscus fruits were finely crushed to about 50 to 100 mesh.

2. 1st extraction step: alcohol extraction step

The pulverized hibiscus fruit powder was put in 70% anhydrous ethanol 8 times by weight and immersed at room temperature for 24 hours to prepare an alcohol extract.

3. Second extraction stage: hot water extraction stage

The alcohol extract was diluted in purified water 5 times the weight and then heated to 80°C to proceed with hot water extraction for 24 hours to obtain a hot water extract.

4. Filtration process

The hot water extract was filtered with a membrane filter (0.45 μm, whatman, membrane filter).

5. One-dimensional separation process

The filtered extract was subjected to primary centrifugation using a centrifuge. At this time, centrifugation was performed at 1,000 g (gravity acceleration) for 10 minutes, and a layer containing collagen was obtained.

6. Second separation and purification step

The primary centrifuged extract was subjected to secondary centrifugation using a centrifuge. At this time, the centrifugation was operated for 1 hour at 80,000 g (gravity acceleration). Among the extracts centrifuged, a layer containing vegetable collagen was identified and separated.

7. The separated vegetable collagen in a liquid form was mixed with a binder (cyclodextrin) 5:5 to prepare vegetable collagen powder through freeze drying (FD) or hot air drying (SD).

Although the preferred embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements of those skilled in the art using the basic concept of the present invention defined in the following claims are also provided. It belongs to the scope of rights.

Claims (10)

A food composition comprising a hibiscus extract comprising plant collagen and a cyclodextrin.
According to claim 1,
The hibiscus extract is a hibiscus fruit extract, food composition.
According to claim 1,
The hibiscus extract is a hot water extract of hibiscus fruit ethanol extract, food composition.
According to claim 1,
The hibiscus extract and the cyclodextrin is contained in a weight ratio of 1: 0.7 to 1.5, food composition.
According to claim 1,
A food composition having a dry powder form.
Pre-treatment step of preparing the dried hibiscus fruit by grinding;
An alcohol extraction step of preparing an alcohol extract by immersing the hibiscus fruit in an alcohol solvent;
A hot water extraction step of mixing the alcohol extract and water and heating to prepare a hot water extract; And
A separation and purification step of producing a hibiscus extract containing vegetable collagen by purifying the hot water extract using a centrifuge.
The method of claim 6,
The heating in the hot water extraction step is 60 to 98

Proceeding to, a method of preparing a hibiscus extract.
The method of claim 6,
The separation and purification step
Filtration process to remove the solid material from the hot water extract to prepare a liquid extract,
A primary separation and purification process for preparing a primary purification by separating the liquid extract in a one-dimensional depth at a gravitational acceleration of 2,000 G or less, and
A second separation and purification process to prepare a hibiscus extract, which is a second purification, by separating the first purification by a two-dimensional seam at a gravitational acceleration of 10,000 G or more.
A method of producing hibiscus extract, which proceeds in this order.
The method of claim 8,
Gravity acceleration applied to the one-dimensional seam separation and the two-dimensional seam separation is 1: 30 to 100 times, a method of manufacturing a hibiscus extract.
The method of claim 6,
The hibiscus extract is a method of preparing a hibiscus extract through a drying process to prepare a hibiscus extract in powder form.

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