KR102066435B1 - Method for manufacturing collagen using olive and olive collagen manufactured by using the same and cosmetic compositions comprising the olive collagen - Google Patents

Abstract

The present invention relates to a method for manufacturing collagen from olives having excellent efficacy in anti-aging by containing a large amount of antioxidants such as vitamin E, tocopherol, polyphenols, and the like, and to a cosmetic composition having excellent efficacy in anti-oxidation and anti-aging by using olive collagen manufactured therefrom. The present invention provides a method for manufacturing collagen using olives, comprising the steps of: extracting olive proteins by acid-treating olives; and hydrolyzing the olive proteins by a protease to produce olive collagen. The present invention also provides olive collagen having antioxidant and anti-aging activity manufactured by using the method, and a cosmetic composition using olive collagen as an active ingredient.

Description

Collagen manufacturing method using olive, olive collagen prepared using the same and a cosmetic composition using the same as an active ingredient {Method for manufacturing collagen using olive and olive collagen manufactured by using the same and cosmetic compositions comprising the olive collagen}

The present invention relates to a cosmetic composition using collagen, and particularly contains a large amount of antioxidants vitamin E, tocopherol, polyphenol, etc., and is rich in protein to produce collagen from olive fruit having excellent efficacy in preventing aging and It relates to a cosmetic composition having excellent efficacy in antioxidant and anti-aging by using olive collagen prepared therefrom.

Today, collagen (Collagen) is an active ingredient used as an important ingredient in medicine, health food, cosmetics, etc. The most abundantly obtained is a fibrous protein, which is an animal collagen distributed in the dermis and connective tissue of the skin.

Animal collagen accounts for 90% of the proteins that make up bone, and is a high molecular protein that forms a polypeptide chain with a molecular weight of about 100,000.

The animal collagen was generally extracted by heating gelatin in water, but recently, a large amount of water-soluble collagen has been prepared using proteolytic enzymes such as pepsin.

However, in the case of animal collagen obtained from animals, there is a risk of human metastasis such as bovine spongiform encephalopathy (BSE) and foot and mouth diseases, which increases the need for collagen produced by alternative sources other than animals. Manufacturing technology is also urgently required.

Accordingly, research on the production of collagen using vegetable or marine raw materials has been made. Plant and marine collagen peptide manufacturing method of Korean Patent Publication No. 10-0488913 is a low molecular weight by enzymatic decomposition of plants and seafood such as fruits, vegetables, seeds, nuts, mushrooms, seaweeds, shellfish, fish, etc. to produce vegetable and marine collagen peptides do. More specifically, the raw material pretreatment step of removing and chopping the foreign material of the raw material to form a raw material of a predetermined size; Citric acid solution immersion step of adding a citric acid aqueous solution to the raw material to form a citric acid aqueous solution of the raw material; An enzyme treatment step of adding a proteolytic enzyme to the aqueous citric acid solution of the raw material and hydrolyzing the protein by maintaining the temperature at 35 to 45 ° C. to form an aqueous collagen peptide solution having a molecular weight of 1000 to 5000; A first filtration step of removing the sludge from the collagen peptide aqueous solution to form a first filtered collagen peptide aqueous solution when collagen reaches a predetermined concentration in the collagen peptide aqueous solution; Enzyme inactivation to heat the temperature of the primary filtration collagen peptide aqueous solution to 85 ℃ or more, and to maintain the temperature for 30 to 60 minutes to inactivate enzymes and sterilize to form a sterilized collagen peptide aqueous solution and Sterilization step; A second filtration step of forming a second filtration collagen peptide aqueous solution by removing foreign matter from the sterilized collagen peptide aqueous solution using a centrifuge; And a drying step of forming a powder collagen peptide by drying the secondary filtered collagen peptide aqueous solution using a drying apparatus. The method of preparing the vegetable / marine collagen peptide is disclosed.

However, the prior art is insufficient to meet the demand for functional cosmetics in recent years because there is no disclosure about the use of the prepared collagen peptide.

On the other hand, the method of preparing a cosmetic composition using the olive oil and gelatin of Korean Patent Publication No. 10-1797638 and the cosmetic composition prepared by this, a cosmetic composition prepared by adding and mixing gelatin in the base composition containing olive oil may be disclosed. In addition, the production of collagen using olive did not provide a single cosmetic composition that can provide both the excellent antioxidant effect of the olive and the anti-aging effect of collagen.

KR 10-0488913 B1 (2005.05.02.) KR 10-1824405 B1 (2018.01.26.) KR 10-2015-0115186 A (2015.10.14.) KR 10-1797638 B1 (2017.11.08.)

Accordingly, the present inventors have focused on solving the limitations and problems of the plant collagen, which has been limited in use in consideration of the above-mentioned matters in general, and particularly contains a large amount of antioxidants such as vitamin E, tocopherol, polyphenol, The invention has been invented as a result of constant effort to develop collagen prepared from olive fruit, which is rich in protein and has excellent efficacy on anti-aging, as a cosmetic raw material.

Therefore, the technical problem and object to be solved by the present invention is to provide a method for producing collagen using olive and olive collagen using the same that can effectively produce collagen from the olive fruit.

In addition, another technical problem and object to be solved by the present invention is to provide a cosmetic composition excellent in antioxidant and anti-aging effect of the olive collagen prepared from the olive fruit as an active ingredient.

The technical problems and objects to be solved by the present invention are not limited to the technical problems and objects mentioned above, and other technical problems and objects not mentioned will be clearly understood by those skilled in the art from the following description.

Collagen production method using an olive according to the present invention for achieving the above object, the step of extracting the olive protein by treating the olive acid; And hydrolyzing the olive protein to produce olive collagen.

In addition, in the method of producing collagen using the olive according to the present invention, the olive is a fruit containing the seed, the fruit from which the seed is removed, the dried fruit including the seed, the dried fruit by removing the seeds, the dried fruit or the dried fruit to compress the oil It is characterized by at least any one of the removal.

In addition, in the collagen production method using the olive according to the present invention, the step of producing the olive protein, the olive flesh is dissolved in an acid solution of pH 2-4 24-72 hours to dissolve the protein and then with a 10um filter Insoluble matters are removed by filtering, and an alkaline aqueous solution is added to adjust pH to 10-13 to coagulate only protein.

In addition, in the collagen production method using the olive according to the present invention, the step of hydrolyzing the olive protein, the olive protein is added to the aqueous solution of pH 6-8 and the protein hydrolase aqueous solution into which the olive protein is added Inject 0.05-5% by weight to 100% by weight, hydrolyze the protein by leaving it for 48-72 hours at 30-40 ° C, and add 0.5-1% by weight of hydrogen peroxide to 100% by weight of the protein-hydrolyzed aqueous solution The activity of the enzyme is stopped to produce an aqueous olive collagen solution, and the olive collagen aqueous solution is passed through an ion exchange resin to remove residual acid or base ions, followed by drying to prepare an olive collagen powder.

In addition, in the method for producing collagen using olive according to the present invention, the proteolytic enzyme is an enzyme having a proteolytic activity produced from Cryobacterium MK-8, proteins in amino acid units along the peptide chain. At least one of the exopeptidase to degrade and the endopeptidase to randomly cleave the protein for molecular weight control.

In addition, in the method of producing collagen using olive according to the present invention, the proteolytic enzyme is, among the exopeptidase pepsin, chymotrypsin, peptidase, trypsin, endopeptidase, carboxypeptidase and aminopeptidase It is characterized by mixing at least one of the molecular weight.

In addition, the olive collagen according to the present invention for achieving the above object is characterized in that the olive collagen prepared by the method for producing collagen using the olive according to the present invention.

In addition, the cosmetic composition comprising the olive collagen according to the present invention for achieving the above object as an active ingredient, 0.01-50% by weight of the collagen powder prepared by the collagen production method using the olive with respect to 100% by weight cosmetics Characterized in that.

In addition, the cosmetic composition as an active ingredient of olive collagen according to the present invention for achieving the above object, consists of a skin, essence, lotion, cream, mask, mist, body cleanser, hair shampoo, hair conditioner and foam cleanser It is characterized by having one formulation selected from the group.

As described above, the present invention contains a large amount of antioxidants such as vitamin E, tocopherol, polyphenols, and olive collagen having excellent antioxidant and anti-aging effects from olive fruit which is rich in protein and has excellent efficacy in preventing skin aging. Can provide.

In addition, the present invention contains a large amount of antioxidants such as vitamin E, tocopherol, polyphenols, antioxidant and anti-aging effect by using olive collagen prepared from olive fruit rich in protein and excellent in preventing skin aging It can provide an excellent cosmetic composition.

In addition, the present invention has the advantage of providing a cosmetic composition of various formulations, such as skin, essence, lotion, cream, mask, mist, body cleanser, hair shampoo, hair conditioner, foam cleanser.

In addition, the present invention can provide a more diverse product range to consumers by developing a new cosmetic raw material called olive collagen, there is an effect of creating a new value in the industry.

1 is a process chart showing a method for producing collagen using olive according to the present invention;
2 is a graph showing the results of measuring the cell growth rate of collagen using olive according to the present invention,
3 is a graph showing the results of measuring the radical scavenging ability to confirm the antioxidant effect of collagen using olive according to the present invention.

Hereinafter, an embodiment of a collagen production method using olive according to the present invention, an olive collagen prepared using the same, and a cosmetic composition using the same as an active ingredient will be described in detail with reference to the accompanying drawings. Embodiments described below are provided to completely inform the scope of the invention to those skilled in the art, the present invention may be implemented in various forms without being limited to the embodiments disclosed below.

Specific details appear in the following description, which is provided to help a more general understanding of the present invention, and is not intended to limit the present invention to the specific embodiments, and all modifications included in the spirit and technical scope of the present invention. It should be understood to include equivalents and substitutes. In the following description of the present invention, if it is determined that a detailed description of a related known function or configuration may unnecessarily obscure the subject matter of the present invention, the detailed description thereof will be omitted.

Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art. Terms such as those defined in the dictionary generally used are defined in consideration of functions in the present invention, which should be construed as concepts consistent with the technical spirit of the present invention and commonly understood or commonly recognized in the art. And, unless expressly defined in this application, is not to be interpreted in an ideal or excessively formal sense.

Example 1 Olive Collagen Preparation

1 is a process chart showing a method for producing collagen using olive according to the present invention. Referring to FIG. 1, collagen (olive collagen) using olive according to the present invention is an olive acid group treatment step 101 and an olive protein valence. It is manufactured through the decomposition process (103).

In Example 1 of the present invention, the olive used was used after pressing the dried olive pulp to remove the oil. In the present invention, the olive may be at least any one of the olive fruit including the seed, the fruit from which the seed is removed, the dried olive fruit including the seed, the seed and the dried fruit, the dried olive fruit or the fruit to be pressed to remove the oil.

In the method for producing olive collagen according to the present invention, the olive acid group treatment step 101 is a step of extracting the protein contained in the olive by acid treatment of the olive, and the olive protein hydrolysis step 103 is performed in step 101 Olive collagen is produced by hydrolyzing the extracted olive protein.

First, in order to obtain olive protein using olives, put olive foil in an acidic aqueous solution of pH 2-4, and immerse for 24-72 hours to dissolve protein contained in olives.

At this time, after the filtering proceeds using a 10um filter to remove the insolubles in the aqueous solution (solution) in which the protein contained in the olive is dissolved, the pH of the solution is 10 using an alkaline aqueous solution to coagulate the protein. Only protein was coagulated by adjusting to -13.

Then, the coagulated protein was added to an aqueous solution of pH 6-8, 0.05-5% by weight of proteolytic enzyme was added to 100 wt% of the coagulated protein solution, and the mixture was left at a temperature of 40-40 hours for 48-72 hours. Protein hydrolysis was advanced. Proteolytic enzymes used enzymes produced from M. K. genus Crygiobacterium, exopeptidase that can break down protein peptide chains by amino acid units, or endopeps that can randomly cleave proteins for molecular weight control. Tidase may also be used. It is also used in combination with exopeptidase pepsin, chymotrypsin, peptidase, trypsin or endopeptidase, carboxypeptidase, aminopeptidase.

Using the above method, 0.5-1% by weight of hydrogen peroxide was added to the aqueous solution of protein hydrolyzed to 100% by weight of the aqueous solution to stop enzymatic activity, and the resulting aqueous solution of olive collagen was passed through the ion exchange resin to remove residual acid or base ions. After removal, pure olive collagen was obtained and dried to prepare a powder.

Experimental Example 1 Anti-Aging Experiment (Measuring Cell Proliferation)

In order to evaluate the anti-aging properties of the olive collagen according to the present invention by confirming the cell activation promoting effect, such as skin cell regeneration promoting and vitalization was carried out an experiment to measure the cell proliferation rate.

Experimental method, first, HDF cultured in a CO ₂ incubator using 10% FBS, 5% P / S (penicillin / Streptomycin) Dulbeco's Modified Eagle's Medium (DMEM) per well 200ul each was dispensed into 96well-plate and stabilized for 24 hours.

Next, the wells were washed once with PBS (Phosphate Buffer Saline), and 198 ul of DMEM containing 1% of FBS (Fetal Bovine Serum) was added per well.

Subsequently, a stock solution is prepared to process 2ul of the sample according to the concentration of cells growing in 198ul of culture medium per well.

For example, if the concentration of the sample to be treated is 10ug / ml, the concentration of the stock solution of the sample is 1mg / ml using DMSO solvent, and the concentration of the sample diluted in the culture solution when 2ul of the sample stock solution is treated with 198ul culture solution. Becomes 10 ug / ml.

Therefore, after making the sample solution for each concentration to be tested, the cells are treated with 2ul each sample.

Negative control was incubated for 48 hours under the same conditions by adding 2ul of DMSO in the same amount as the sample. Positive control was treated with 1 mM of ascorbic acid.

After 24 hours of incubation, 10 μl of MTT Assay kit (3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide) was added to the control medium and the treated sample, followed by 2 hours of incubation. Absorbance was measured at 450 nm, which is shown in FIG. 2.

Figure 2 is a graph showing the results of measuring the cell growth rate of olive collagen according to the present invention, as a result of absorbance measurement, as shown in Table 1 below all three samples of Example 1 than the positive control (Positive Control) It showed high cell proliferation rate, especially 2 times higher cell proliferation rate than negative control. Therefore, it was confirmed that the activity of the cell proliferation rate was confirmed, and it can be evaluated that the olive collagen according to the present invention has a high anti-aging characteristics by confirming the cell activation promoting effect, such as skin cell regeneration and vitalization. Table 1 summarizes the cell proliferation rate by concentration of the olive collagen sample according to the present invention.

Cell growth activity (%) Cell growth rate by sample concentration (%) sample Example 1 Proper concentration (mg / ml) 0.5 1.0 2.5 Cell proliferation rate (%) 205% 223% 220%

Experimental Example 2: Antioxidant Experiment (DPPH Assay)

DPPH (1,1-diphenyl-2-picrylhydrazyl) is a water-soluble substance with chemically stabilized free radicals, which has a maximum absorbance near 515-520 nm, and gives off electrons when it encounters a substance with antioxidant activity. ) Is discolored as it disappears, so antioxidant activity can be easily observed with the naked eye. Experimental Example 2 was to evaluate the antioxidant efficacy that can be provided when the olive collagen sample according to the present invention is used as a functional cosmetics using a DPPH solution.

Experimental method is prepared by first dissolving the sample in DMSO for each concentration.

Next, 200ul of 180ul 1mM DPPH solution and 20ul of sample for each concentration were mixed in a 96well-plate and incubated at room temperature (25 ° C) for 10 minutes.

Blank was mixed with 20ul of methanol in 180ul of 1mM DPPH, and positive control was treated with ascorbic acid (1mM, about 90% inhibition).

Absorbance measurements for evaluating PPH radical scavenging activity were measured at 520 nm (or 565 nm) with a microplate reader to measure DPPH radical scavenging ability, which is shown in FIG. 3.

Figure 3 is a graph showing the results of measuring the radical scavenging activity showing the antioxidant activity for confirming the antioxidant effect of olive collagen according to the present invention, the antioxidant inhibition test results, in the olive collagen sample of Example 1 1-2.5mg / ml It showed an antioxidant effect of 55%, and thus olive collagen according to the present invention was confirmed that the antioxidant activity. Table 2 summarizes the antioxidant activity according to the concentration of the olive collagen sample according to the present invention.

Antioxidant activity (%) Antioxidant Activity by Sample Concentration (%) sample Example 1 Proper concentration (mg / ml) 0.5 1.0 2.5 Activity (%) 82% 45% 42%

In the antioxidant inhibition experiment of Figure 3 samples 1-2.5mg / ml showed more than 55% antioxidant efficacy. Accordingly, it can be confirmed that the sample has antioxidant activity.

As described above, the olive collagen according to the present invention can be utilized as a raw material for aging and antioxidant-related cosmetics.

<Experimental example 3: skin soothing (irritating effect)>

Cell culture technology was used to comparatively evaluate the skin irritation-releasing effect of olive collagen according to the present invention, and was used by being distributed from fibroblasts (Korea Cell Line Bank, Korea), which are human skin cells.

The experiment was carried out by adding sodium lauryl sulfate (SLS Sigma-Aldrich), which is a cause of skin irritation, to the cultured cells, with an SLS concentration of 0.002% by weight (all skin cells were killed at this concentration). The degree of inhibition of apoptosis was evaluated as a stimulating effect.

Fibroblasts were dispensed at 1 × 10 ⁵ concentrations in 96 wells, incubated for 24 hours, followed by addition of 0.002 wt% SLS alone, 0.002 wt% SLS and 0.1 wt% of the raw materials of Examples 1 and 2, respectively. Further incubation for 24 hours.

After completion of the culture, MTT assay (Sigma-Aldrich) reagent was added to compare the viability of the cells, and the degree of cell death was measured using a spectrophotometer (OPIZEN 3220UV Mecasys) to measure 570 nm absorbance. By calculating the cell viability (%) according to the results are summarized in Table 3 below.

As described above, it can be confirmed that the olive collagen according to the present invention suppresses apoptosis caused by SLS, thereby relieving skin irritation that may occur with the cosmetic substrate when prescribed with the cosmetic substrate.

Next, as an application of the cosmetic composition that can be provided in a variety of formulations using the olive collagen according to the present invention as a skin, essence, lotion, cream, mask, mist, body cleanser, hair shampoo, hair conditioner, Present a foam cleanser. Application examples described below are provided to completely inform the scope of the invention to those skilled in the art, the present invention can be implemented in various forms without being limited to the applications disclosed below.

<Application Example 1: Skin Type Cosmetic Composition>

Skin type cosmetic composition comprising the olive collagen according to the present invention as an active ingredient purified water 40-90% by weight, olive collagen 0.01-50% by weight, methylgluses-20 0.5-2% by weight, betaine 0.5-2% by weight, 0.05-2% by weight carbomer, 0.01-2% by weight hydroxyethyl cellulose, 0.01-2% by weight disodium ethane, 0.01-2% by weight paraoxybenzoic acid methyl, 1-5% by weight glycerin, butylenegly 0.5-5% by weight of Kohl, 0.01-2% by weight of Fiji-60 Hydrogenated Caster Oil, 0.01-1% by weight, 0.05-1% by weight of phenoxyethanol, 0.01-1% by weight of potassium hydroxide .

<Application Example 2: Essence Type Cosmetic Composition>

Essence-type cosmetic composition using an olive collagen as an active ingredient according to the present invention 40-85% by weight purified water, 0.01-50% by weight of olive collagen, 0.1-2% by weight carbomer, 0.01-1% by weight of disodium ethane, Xanthan gum 0.1-0.5% by weight, sebum-32 0.5-5% by weight, glycerin 5-10% by weight, dipropylene glycol 1-5% by weight, ethanol 1-5% by weight, Fiji-60 hydrogenated 0.1-1% by weight of castor oil, 0.1-1% by weight of phenyltrimethicone, 0.1-0.5% by weight of methyl paraoxybenzoate, 0.1-0.5% by weight of phenoxyethanol, and 0.1-0.5% by weight of triethanolamine.

<Application Example 3: Lotion Type Cosmetic Composition>

The lotion-type cosmetic composition comprising olive collagen as an active ingredient according to the present invention is purified water 40-85% by weight, olive collagen 0.01-50% by weight, carbomer 0.05-0.5% by weight, disodium idiate 0.01-0.1% by weight, Paraoxybenzoic acid methyl 0.05-0.5% by weight, glycerin 1-5% by weight, butylene glycol 1-5% by weight, liquid paraffin 5-10% by weight, glyceryl stearate / Fiji-100 stearate 0.1-3 weight %, Glyceryl stearate 0.1-3 wt%, Polysorbate 80 0.1-3 wt%, Stearic acid 0.1-3 wt%, Paraoxybenzoic acid propyl 0.01-1 wt%, Potassium hydroxide 0.01-1 wt% Included.

<Application Example 4: Cream Cosmetic Composition>

Creamy cosmetic composition comprising olive collagen according to the present invention as an active ingredient 30-75% by weight purified water, 0.01-40% by weight olive collagen, 0.05-0.5% by weight carbomer, 1-10% by weight glycerin, butylene glycol 1-5 wt%, 0.01-0.1 wt% disodium ethane, 0.05-0.5 wt% paraoxybenzoic acid methyl, 0.5-1 wt% stearic acid, 1-5 wt% cetearyl alcohol, glyceryl stearate 0.5-5% by weight, 0.5-1% by weight polysorbate, 5-10% by weight cetylethylhexanoate, 1-10% by weight paraffin, 0.05-0.5% by weight propyl paraoxybenzoate, 0.05-% by weight triethanolamine 0.5% by weight, 0.01-0.1% by weight fragrance is included.

<Application Example 5: Cosmetic Composition for Mask Pack>

Cosmetic composition for a mask pack using olive collagen according to the present invention is purified water 40-90% by weight, olive collagen 0.01-50% by weight, methylgluses-20 0.5-5% by weight, betaine 0.1-0.5% by weight, 0.5-5% by weight carbomer, 0.01-0.5% by weight hydroxyethyl cellulose, 0.01-0.1% by weight disodium ethane, 0.1-0.5% by weight paraoxybenzoic acid methyl, 1-10% by weight glycerin, butylenegly 1-5% by weight of Kohl, 0.1-0.5% by weight of Fiji-60 Hydrogenated Caster Oil, 0.01-0.1% by weight, 0.1-0.5% by weight of phenoxyethanol, 0.01-0.1% by weight of potassium hydroxide .

<Application Example 6: Cosmetic Composition for Mist>

Mist cosmetic composition using an olive collagen as an active ingredient according to the present invention 40-95% by weight of purified water, 0.01-50% by weight of olive collagen, methylgluses-20 0.5-5% by weight, sebum-32 1-0.5% %, Betaine 0.1-0.5%, Hydroxyethyl Cellulose 0.01-0.5%, Disodium ID, 0.01-0.1%, Clofenesin 0.1-1%, Glycerin 1-10%, Butylene Lycol 1-5%, Polysorbate 20 0.1-0.5%, Flavor 0.01-0.1%, Phenooxyethanol 0.1-0.5%, Citric acid 0.05-0.5%, Sodium citrate 0.1-0.5% % Is included.

<Application Example 7: Cosmetic Composition for Body Cleanser>

The cosmetic composition for body cleanser using olive collagen as an active ingredient according to the present invention is purified water 40-85% by weight, olive collagen 0.01-50% by weight, sodium laureth sulfate 5-15% by weight, sodium lauryl sulfate 5-15% by weight %, Disodium ID, 0.05-0.3% by weight, Methyl paraben 0.1-0.3% by weight, Disodium ID, 0.01-0.1% by weight, Cocamide M 1-5%, Cocamidopropylbetaine 1-5 Weight percent, flavor 0.1-0.5 weight percent, polysorbate 20 0.5-5 weight percent, sodium chloride 0.1-1 weight percent.

<Application Example 8: Cosmetic Composition for Hair Shampoo>

Cosmetic composition for hair shampoos using olive collagen as an active ingredient according to the present invention 40-85% by weight of purified water, 0.01-50% by weight of olive collagen, 0.01-0.1% by weight of disodium ethane, sodium salicylate 0.01-0.5 Weight%, Polyquaternium-10 0.01-0.5 weight%, Sodium laureth sulfate, Glycol distearate, Lauramid die, Cocamide M 5-20 weight%, Ammonium lauryl sulfate 1-10 weight% , 1-5% by weight of ammonium laureth sulfate, 1-5% by weight of cocamidopropyl betaine, 0.1-0.5% by weight, 0.1-0.5% by weight of methyl paraben, 0.1-0.5% by weight of klimbazole, sodium chloride 0.5-5 Wt%, phenoxyethanol 0.1-0.5 wt%, cocamide Diei 0.5-5 wt%

<Application Example 9: Cosmetic Composition for Hair Conditioner>

Cosmetic composition for hair conditioner using olive collagen as an active ingredient according to the present invention is purified water 40-90% by weight, olive collagen 0.01-50% by weight, behentrimonium chloride 1-5% by weight, cetearyl alcohol 1-5% by weight %, Glyceryl stearate 0.1-1% by weight, methyl paraben 0.1-0.5% by weight, paraffin 0.5-5% by weight, dimethicone 1-5% by weight, glycerin 1-5% by weight, perfume 0.1-1% by weight Included

<Application Example 10: Cosmetic Composition for Foam Cleanser>

The cosmetic composition for foam cleanser using olive collagen as an active ingredient according to the present invention is purified water 40-65% by weight, olive collagen 0.01-50% by weight, glycerin 1-10% by weight, cocamidopropyl betaine 0.5-5% by weight, 0.1-0.5% by weight of disodium ID, 1-10% by weight of stearic acid, 1-15% by weight of mystic acid, 1-15% by weight of lauric acid, 2-10% by weight of potassium chloride, flavor 0.1-0.5 % By weight is included.

As described above, using the olive collagen according to the present invention as an active ingredient, skin, essence, lotion, cream, mask, mist, body cleanser, hair shampoo, hair conditioner, foam cleanser, etc. It can provide a cosmetic composition having.

On the other hand, in the detailed description of the present invention has been described in detail with reference to the preferred embodiment referenced by the accompanying drawings, various modifications are possible without departing from the scope of the invention. Therefore, the scope of the present invention should not be limited to the described embodiments, but should be determined not only by the scope of the following claims, but also by the equivalents of the claims.

Claims (9)

The olives were added to an acidic solution of pH 2-4 for 24-72 hours to dissolve the protein contained in the olive, filtered with a 10um filter to remove insoluble matters, and the pH was adjusted to pH 10-13 by adding an alkaline aqueous solution. Coagulating bay to extract olive protein;
The coagulated olive protein is added to an aqueous solution of pH 6-8, an enzyme having proteolytic activity produced from Cryobacterium MK-8, an exopeptidase that breaks down proteins into amino acid units according to peptide chains, and At least one of endopeptidase that randomly cleaves proteins for molecular weight control is used as a protease, and is added in an amount of 0.05-5% by weight based on 100% by weight of the aqueous solution in which the olive protein is added. Hydrolyzing the protein by leaving it to stand for 48-72 hours at temperature;
Injecting 0.5-1% by weight of hydrogen peroxide relative to 100% by weight of the aqueous solution of the hydrolyzed protein to stop the activity of the enzyme to produce an olive collagen aqueous solution; And
The olive collagen aqueous solution is passed through an ion exchange resin to remove residual acid or base ions and dried to produce an olive collagen powder.
The method of claim 1, wherein the olive,
A method of producing collagen using olives, characterized in that at least one of the fruit, including the seed, the fruit from which the seed is removed, the dried fruit, including the seed, the dried fruit, and the dried fruit or the dried fruit to squeeze the oil to remove the oil.
delete delete delete The method according to claim 1, wherein the proteolytic enzyme,
An exopeptidase pepsin, chymotrypsin, peptidase, trypsin, endopeptidase, carboxypeptidase and aminopeptidase by mixing at least one of the collagen production method using an olive, characterized in that to adjust the molecular weight.
Olive collagen prepared by the method of any one of claims 1, 2 and 6.
A cosmetic composition comprising olive collagen as an active ingredient, wherein the olive collagen of claim 7 is contained in an amount of 0.01-50% by weight based on 100% by weight of the cosmetic.
The method of claim 8, wherein the cosmetic composition,
A cosmetic composition comprising olive collagen as an active ingredient, which has one formulation selected from the group consisting of skin, essence, lotion, cream, mask, mist, body cleanser, hair shampoo, hair conditioner and foam cleanser.

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