KR20030064902A - Toothpaste containing a natural antifungal ingredient from wasabia japonica - Google Patents

Abstract

PURPOSE: Provided is a tooth paste containing fragrance component from Wasabia japonica which has strong antibiotic activity against the bacteria causing periodontal diseases such as periodontitis and decaying. CONSTITUTION: The tooth paste comprises 0.5-1.5wt.% of antibiotic component extracted from leaves and stems of Wasabia japonica by supercritical fluid extraction method, together with other usual components of tooth paste.

Description

Toothpaste containing horseradish's natural antibacterial ingredients {TOOTHPASTE CONTAINING A NATURAL ANTIFUNGAL INGREDIENT FROM WASABIA JAPONICA}

The present invention relates to a toothpaste containing a natural antibacterial component of wasabi and a method for producing the same. More specifically, in the present invention, Wasabia japonica has a strong bactericidal effect on the bacteria that cause periodontal disease, particularly periodontitis or tooth decay, and is effectively added to toothpaste by adding to toothpaste. It relates to toothpaste having bactericidal or antimicrobial activity against microorganisms in the oral cavity as well as the smell of.

Typically, toothpaste is composed of a major component, such as abrasives, moisturizing agents, surfactants, binders, sweeteners, fragrances, antibacterial agents, pigments, stabilizers and the like and other necessary ingredients.

Herein, the antimicrobial agent used in the manufacture of toothpaste among the above components is used to prevent the contamination of the contents or separation or deterioration due to bacteria or fungi during the production, storage, distribution and use of the toothpaste product. It acts as a cause of tooth decay, periodontal, gingivitis, and acts as a bactericidal or inhibits metabolism of the microorganisms in the oral cavity causing bad breath. As antiseptics, parahydroxybenzoate, phenol chloride, benzoic acid And salts thereof, fluorine compounds, sodium dihydroacetate, strontium chloride, chlorhexidine, hinokthiol, and protein, and the like, and usually two or more thereof are used in combination.

However, as described above, commonly used antimicrobial agents have problems in toxicity, stability, and taste. The antimicrobial agent contained in the toothpaste composition was inactivated by surfactants or abrasives, and the activity of the antimicrobial agent was often lowered, and the oral mucosa was stimulated by adding too much antimicrobial agent to prevent the degradation or inactivation of the antimicrobial activity. It may cause swelling, blisters or erythema, or may cause central nervous system disorders. In the case of fluorine compounds, there are disadvantages of causing spots on teeth.

An object of the present invention to solve the problems of the conventional toothpaste as described above is to provide an antimicrobial toothpaste comprising a natural antibacterial component extracted from the leaves and stems of horseradish in a conventional toothpaste.

Toothpaste containing the antibacterial component of wasabi according to the present invention,

First, it is recognized that the natural antibacterial component extracted from the leaves and stems of horseradish has a strong antibacterial activity against microorganisms, especially Streptococcus mutans , which is known as the causative agent of tooth decay. By doing so, it is not only to increase the antiseptic power of the product itself, but also to provide an antibacterial toothpaste having excellent sterilizing power against oral microorganisms,

Second, by adding natural antibacterial ingredient of wasabi to toothpaste, even if a small amount of antimicrobial agent is used to increase the antiseptic power of the product, it minimizes the side effects caused by excessive use of preservatives, and also used in the manufacturing of toothpaste Minimize side effects to oral mucosa and human body caused by overdose

Third, it is possible to prevent tooth decay, periodontal, and gingivitis by the bactericidal action on oral microorganisms, and at the same time to provide antibacterial toothpaste with enhanced antibacterial ability to prevent bad breath by using the deodorizing action of horseradish.

Toothpaste containing the antimicrobial component extracted from wasabi was prepared by adding wasabi extract extracted by a supercritical fluid extraction method to a conventional toothpaste composition.

Supercritical fluid extraction has been used efficiently in basic research and actual separation processes aimed at separating and refining mixtures, and is useful for reducing thermal denaturation, solvent retention, and loss of extract components. It is particularly suitable for the present invention because it has the advantage of using chemically inert, non-toxic and non-combustible carbon dioxide for the extraction by the method, and easy removal of the solvent after extraction.

In a preferred embodiment of the present invention, it is preferable to add 0.5 to 1.5% by weight of horseradish leaves or stem extracts to the conventional toothpaste raw materials.

In the present invention, the leaves or stems of wasabi extract using a known supercritical fluid extraction method to produce the desired natural antibacterial component. To explain this roughly, the leaves and stems of dried wasabi (dry or lyophilized) finely pulverized and extracted using a supercritical fluid extractor, wherein supercritical fluid is carbon dioxide (CO ₂ ), The extraction temperature is 35 to 70 ° C and the extraction pressure is in the range of 100 to 600 bar, preferably in the range of 100 to 400 bar.

The method for producing the toothpaste of the present invention can be prepared by containing the horseradish extract of the present invention in a common toothpaste composition.

Conventional toothpaste components include calcium carbonate, calcium monohydrogen phosphate, precipitated silica, hydrous alumina, silica gel, insoluble sodium metaphosphate, aluminum silicate, 20 to 60% by weight of these abrasives alone or in combination of two or more thereof. Preferably from 20 to 50% by weight is used.

As the humectant, 20 to 60% by weight of glycerin, sorbitol solution, amorphous sorbitol solution, xylitol, propylene glycol, polyethylene glycol, etc. alone or in combination of two or more thereof, preferably glycerin, amorphous sorbitol solution, polyethylene glycol, 20 to 50% by weight of xylitol or the like or a mixture of two or more thereof is used.

Examples of the binder include carrageenan, xanthan gum, carboxymethylcellulose sodium, hydroxyethylcellulose, acrylic acid polymers, carboxyvinyl polymers, sodium alginate, and the like, or 0.1 to 3.0% by weight, preferably carrageenan, hydroxyethyl The cellulose acrylic acid polymer alone or in combination of two or more thereof is used in an amount of 0.5 to 1.5 wt%.

As the foaming agent, an anionic surfactant such as sodium lauryl sulfate or sodium lauryl sarcosinate, sorbitan fatty acid ester, polyoxyethylene hardened castor oil, polyoxyethylene polyoxypropylene-based condensed polymer material, etc. may be used alone or in a mixture of 0.5 to 5 Wt%, preferably sodium lauryl sulfate alone or polyoxyethylene polyoxypropylene condensation polymer material is used in a mixed amount of 0.5 to 2.5% by weight.

The sweeteners include saccharin sodium, aspartame, licorice acid and the like, and preferably 0.05 to 0.5% by weight is used alone or in combination of two or more thereof.

As the preservative, paraoxy benzoic acid ester, benzoic acid, sodium benzoate is used alone or mixed, and as a fragrance, a suitable amount of peppermint oil, spearmint oil, menthol, carbon, etc. is mixed. As a pH adjuster, phosphoric acid, sodium phosphate, citric acid, Sodium citrate, succinic acid, sodium succinate, tartaric acid, sodium stannate and the like are used, the pH range is 5 to 8 suitable, titanium oxide is used as a brightener, 0.1 to 2% by weight is appropriate.

In addition, the toothpaste composition of the present invention is trinexamic acid, allantoin, vitamins, amine fluoride, tin fluoride, chlorhexidine, triclonic acid, ursodesoxycholine acid, capronic acid, bamboo salt, yellow white, raw guinea, Gil Kyung It may also contain medicaments such as extracts such as molds and gardenia.

Hereinafter, the present invention will be described in detail with reference to Examples and Experimental Examples. However, the following Examples and Experimental Examples are illustrative of the present invention and do not limit the scope of the present invention.

Example 1

After finely pulverizing the leaves and stems of the three-year-old freeze-dried horseradish, 100 g were placed in a supercritical fluid extractor, and the carbon dioxide of the fluid supply cylinder was pressurized to 400 bar by a syringe pump through a filter, while ethanol was added to the same solvent chamber. It is pressurized by pressure to adjust the 3-way valve to send carbon dioxide: ethanol to the extractor in the ratio of 9: 1, and maintain the oil jacket at 45 ℃ to maintain the internal temperature at 40 ± 5 ℃ 20 minutes 5L / min Supercritical fluid extraction was performed by flowing at a flow rate. The horseradish extract extracted from the separator weighed about 30 g.

Example 2

Wasabi antibacterial component-containing toothpaste of the present invention was prepared with the following composition. The content is weight percent.

Calcium Dihydrogen Phosphate 40

Sorbitol 30

Alkyl sodium sulfate 1.50

Saccharin Sodium 0.10

Paraoxybenzoic acid ester 0.20

Carrageenan 1.00

Spices 1.0

Horseradish extract 1.0 of Example 1

Adding purified water 100

Experimental Example 1

Analysis of the aroma component of horseradish extract prepared in Example 1 can be performed by a number of methods, in the present invention freeze-dried wasabi leaves and stems, and extracted by essential oil extraction method by supercritical extraction analysis by GC / MS It was. The results are shown in Table 1 below:

Component Content of Wasabi Extract Isothiocyanate (NCS) Horseradish stem leaf Allyl NCS 62.0 76.0 n-Butyl NCS 1.0 1.2 3-Butenyl NCS 0.2 0.9 4-Pentenyl NCS 2.2 2.6 5-Hexenyl NCS 1.0 1.9 -Phenylethyl NCS - - 5-Methylthipentyl NSC 0.9 0.4 6-Methylthiohexyl NCS 8.8 3.8 7-Methylthioheptyl NCS 2.0 1.1 5-Methylsulfinylpentyl NCS 1.0 1.4 6-Methylsulfinylhexyl NCS 8.4 18.0 Palmitic acid 9.2 5.2 7-Methylsulfinylheptyl NCS 1.5 3.6 Linolenic acid 14.0 18.0 Oleic acid

As confirmed in Table 1, the natural antibacterial component of the leaves and stems of horseradish used in the present invention analyzed the fragrance components from the leaves and stems of wasabi cultivated for three years, a strong antibacterial among the components Natural antibacterial ingredient, the main component of which contains about 60% of allyl isothiocyanate, the strongest antibacterial ingredient, 6-methylthiohexyl isothiocyanate, which is a unique ingredient of wasabi, 8 %, 6-methylsulfinylhexyl iso is known to increase the activity of glutathione b-transferase and quinone reductase, enzymes that inhibit their formation in the early stages of chemical stress or tumorigenesis in the liver It can be embodied with 12 kinds of essential oils such as thiocyanate about 7.8%.

Experimental Example 2

In order to examine the bactericidal power of the general wasabi or periodontal bacteria of the horseradish extract component of the present invention according to the analysis result of Experimental Example 1, a phenolic acid count method (Rideal & Walker 1903) was used. .

Chungchigyun received pre-sale from Korea seed Association (Streptococcus mutans) a pure culture by, and at the same time bacteria include E. coli O-157 as the test bacterium (Escherichia coli O-157: H7 ATCC 43889) and sikjungdokgyun of Vibrio parahaemolyticus (Vibrio parahaemolyticus ATCC 2210001), Staphylococcus aureus ATCC 25923) The results showed that the coefficient of phenol of the component found to be excellent is shown in Table 2.

Phenol Coefficient of Wasabi Extract sample Phenol Coefficient Streptococcus mutans Escherichia coli O-157: H7 Vibrio parahaemolyticus Staphylococcus aureus Coal mountain 1.0 1.0 1.0 1.0 Horseradish Stem Extract 1.8 1.8 2.4 1.4 Horseradish Leaf Extract 2.4 2.2 2.9 1.9

As shown in the experimental results of the phenolic acid coefficient of Table 2, the natural antibacterial components of horseradish stems and leaves showed high phenolic acid coefficients for all four test bacteria, especially Escherichia coli O-157 and Staphylococcus aureus, which are common bacteria. The bactericidal power of Streptococcus mutans , which is known to cause periodontal disease, including dental caries, is higher.

Experimental Example 3

In order to determine the antimicrobial activity of the toothpaste with horseradish extract added wasabi extract of the present invention was tested as follows (agar well method).

Toothpaste according to Example 2, which was added with 1.0% of horseradish extract (leaves or stems), and 0.02% solution of methylpara-hydroxybenzoic acid as a preservative as a control sample, and the agar medium coated with test bacteria was used. 5 mm of wells were made, and then the sample was placed in the wells, and incubated at about 37 ° C. for 48 hours, and the size of the transparent ring around the wells was measured. The results are shown in Table 3 below.

Antimicrobial Effect of Toothpaste Added with Wasabi Extract sample Transparent ring (proliferative ring, cm) Streptococcus mutans Escherichia coli O-157: H7 Vibrio parahaemolyticus Staphylococcus aureus 0.02% methylpara-hydroxybenzoic acid solution 1.2 1.1 1.6 1.0 Horseradish Stem Extract 1.4 1.2 1.6 1.1 Horseradish Leaf Extract 1.6 1.4 2.5 1.0

As a result of the above results, it was found that the toothpaste added with the components extracted by the supercritical extraction method from horseradish leaves and stems has an excellent antiseptic disinfectant than the antiseptic fungicides of the conventional toothpaste.

Toothpaste containing a natural antibacterial component from wasabi according to the present invention, because it has a strong antibacterial activity against microorganisms such as caries bacteria, can contribute to oral hygiene, increase the antiseptic power of the product itself according to the overdose of conventional chemical antimicrobial agents The problem can be solved and it can be used more stably.

Claims (1)

An antimicrobial toothpaste comprising 0.5 to 1.5% by weight of an antimicrobial component extracted by using supercritical fluid extraction from leaves and stems of Wasabia japonica .