KR20030034279A - Antibiotic composition for a diaper - Google Patents

Abstract

PURPOSE: A composition for applying to the disposable diaper is provided to have the antiallergic activity, the anti-inflammatory activity and the antibacterial activity by treating the top sheet of the disposable diaper with Portulacaceae extracts. CONSTITUTION: The composition for applying to the disposable diaper comprises the Portulacaceae extracts. The Portulacaceae extracts contain the extracts or powders thereof obtained by adding one or at least two selected from the group consisting of water, ethanol, methanol, propanol, butanol, acetone, ethyl acetate, hexane, benzene, chloroform, glycerin, butylene glycol, propylene glycol, hydrous ethanol, hydrous methanol, hydrous propanol, hydrous butanol, hydrous glycerin, hydrous butylene glycol and hydrous propylene glycol to the Portulacaceae, and extracting it at the temperature of 95 deg.C for 4 to 20 hours or at the temperature of 5 to 40 deg.C for 1 to 15 days.

Description

Disposable diaper application composition with anti-allergic, anti-inflammatory and antibacterial properties {Antibiotic composition for a diaper}

The present invention relates to a composition for applying a disposable diaper having the functions of anti-allergic, anti-inflammatory and antibacterial, and more particularly, to a disposable diaper containing or treated with an anti-allergic, anti-inflammatory and antimicrobial activity. It is about.

In general, disposable diapers (for infants and adults) have a structure including an absorber in which wood pulp and superabsorbent polymer are mixed between the top sheet and the back sheet and additionally have a waist bend or leg band depending on the application.

The diapers currently on the market are focused on breathability, fit, or absorbency of secretions, and there are very few products to eliminate or reduce side effects caused by wearing diapers (eg diaper rash or eczema).

Therefore, the present invention is to provide a disposable diaper coating composition for treating or containing anti-allergic, anti-inflammatory and antimicrobial activity in the diaper product itself so as to exhibit anti-allergic, anti-inflammatory and antimicrobial activity on the skin.

The present invention is to exhibit anti-allergic, anti-inflammatory and antimicrobial activity in a disposable diaper, more specifically, the anti-allergic by treating the upper sheet of the disposable diaper with 0.5% to 50% of the weight of the nonwoven fabric by using a chorus extract. It provides a composition characterized by exhibiting anti-inflammatory and antibacterial activity.

In addition, the present invention provides a disposable diaper to which the gusset extract is directly applied to the upper sheet or the absorbent body.

Hereinafter, the present invention will be described in detail.

March is a year-round grass, 15-30cm high. It is reddish red and many branches are spread diagonally to the side. In summer, small yellow flowers gather at the end of branches. In the outpost, there is almost the same amount of dopamine, and less dopa, as 0.25% of noradrenaline C8H11O3N. There are also potassium salts, large amounts of organic acids, cardiac glycosides andhraquinone glycosides, and alkaloid reactions. (The Ingredients and Uses of Herbs. Interest, P227, 1999)

Machise extract of the present invention is as follows. First, Machi (Sea) is extracted by weight as solvent, water, ethanol, methanol, propanol, butanol, acetone, ethyl acetate, hexane, benzene, chloroform, glycerin, butylene glycol, propylene glycol, hydrous ethanol, hydrous methanol, hydrous 1-20 times by volume of at least one solvent selected from the group consisting of propanol, hydrous butanol, hydrous glycerin, hydrous butylene glycol and hydrous propylene glycol is added. Extraction method is a method of extracting by heating at 50-95 ° C for 4-20 hours or extracting active ingredient at 5-37 ° C for 1-15 days while cooling condenser is installed to prevent evaporation of solvent. Can be. In addition, the extracted Macula (sorbi) extract is concentrated under reduced pressure using a distillation apparatus equipped with a cooling condenser to prepare a concentrate or powdered Maca (sea) extract, or β-CD (cyclodextrin), hydroxypropyl-β- CD, malto-dextrin, lactose, and silica powder were mixed to prepare a powdery portuguese extract. The non-woven fabric treated by treating the gusset extract extracted in the above manner to the nonwoven fabric is applied to the disposable diaper as a top sheet. When the nonwoven fabric is treated, the gusset extract is used in an amount of 0.5% to 50% by weight of the nonwoven fabric.

Materials used in disposable diapers according to the present invention are as follows. The top sheet used a polypropylene nonwoven fabric, which can be treated with a chord extract solution or applied as an extract powder. The absorber is composed of wood pulp or superabsorbent resin, and the absorber can be treated with a chord extract solution or applied as an extract powder.

The following examples illustrate the content of the invention, but the content of the invention is not limited thereto.

Example 1.

1 kg of washed Machi strings with purified water was added to 6 kg of brine ethanol, and extracted at room temperature for 6 days with an extractor.

Example 2-9.

Using the solvent of Table 1 below and extracting in the same manner as in Example 1 and the results are shown in Table 1 below.

TABLE 1

Solvent Yield [kg] Example 2. water 5.3 Example 3. ethanol 5.2 Example 4. glycerin 4.9 Example 5. Butylene glycol 4.9 Example 6. Propylene glycol 5.3 Example 7. Hydrous propylene glycol 5.4 Example 8. Hydrous butylene glycol 5.2 Example 9. Glycerin 5.4

Example 10.

1kg of gusset string washed with purified water was put in 6kg of hydrated butylene glycol, and a cooling condenser was installed to prevent evaporation of the solvent, and then heated at 80 ° C. for 3 hours, followed by filtration with 400 mesh filter cloth, followed by filtration with a filter of 0.45 μm. 5.3 kg of extract was obtained.

Example 11-18.

Using the solvent of Table 2 below and extracting in the same manner as in Example 10 and the results are shown in Table 2.

TABLE 2

Solvent Yield [kg] Example 11. water 5.4 Example 12. ethanol 5.3 Example 13. glycerin 5.7 Example 14. Butylene glycol 5.5 Example 15. Propylene glycol 5.5 Example 16. Hydrous propylene glycol 5.6 Example 17. Ethanol 5.3 Example 18. Glycerin 5.6

Example 19.

1kg of Machihyun washed with purified water was added to 6kg of brine ethanol and extracted with an extractor at room temperature for 6 days, filtered through a 400 mesh filter cloth, and warmed to concentrate more than 50% of the initial volume. Filtration with a 400 mesh filter cloth, followed by a filter of 0.45㎛. The filtered solution was dried under reduced pressure using a distillation apparatus equipped with a cooling condenser to obtain 21 g of a powdered portuguese extract.

Example 20-29.

Using the solvent of Table 3 below and extracting in the same manner as in Example 19 and the results are shown in Table 3.

TABLE 3

Solvent Solid content [g] Example 20. water 18 Example 21. ethanol 24 Example 22. Methanol 23 Example 23. Propanol 20 Example 24. Butanol 20 Example 25. Acetone 19 Example 26. Ethyl acetate 18 Example 27. Hexane 18 Example 28. benzene 19 Example 29. chloroform 20

Example 30.

1kg of Machihyun washed with purified water was added to 6kg of hydrous ethanol, and extracted at room temperature with an extractor for 6 days, filtered through a 400 mesh filter cloth, and then filtered with a filter of 0.45㎛. After warming and concentrating at 50% or more, the mixture was mixed with 30 g of malto dextrin, and then spray-dried to obtain 46 g of extract of powdered portella.

Examples 31-41.

The solvent of Table 4 was used and extracted in the same manner as in Example 30, and malto dextrin was used as an excipient and the results are shown in Table 4 below.

TABLE 4

Extraction solvent Dry weight of total extract [g] Example 32. water 40 Example 33. ethanol 43 Example 34. Methanol 45 Example 35. Propanol 43 Example 36. Butanol 42 Example 37. Acetone 46 Example 38. Ethyl acetate 46 Example 39. Hexane 39 Example 40. benzene 39 Example 41. chloroform 41

Examples 42-52.

The solvent of Table 5 was used and extracted in the same manner as in Example 30, and the results are shown in Table 5 below using β-CD (cyclodextrin) as an excipient.

TABLE 5

Extraction solvent Dry weight of the final extract [g] Example 42. water 43 Example 43. ethanol 45 Example 44. Methanol 43 Example 45. Propanol 42 Example 46. Butanol 39 Example 47. Acetone 38 Example 48. Ethyl acetate 39 Example 49. Hexane 38 Example 50. benzene 38 Example 51. chloroform 39 Example 52. Ethanol 46

Examples 53-63.

The solvent of Table 6 was used and extracted in the same manner as in Example 30, and the results are shown in Table 6 using hydroxypropyl-β-CD (cyclodextrin) as an excipient.

TABLE 6

Extraction solvent Dry weight of the final extract [g] Example 53. water 43 Example 54. ethanol 41 Example 55. Methanol 43 Example 56. Propanol 45 Example 57. Butanol 40 Example 58. Acetone 44 Example 59. Ethyl acetate 39 Example 60. Hexane 38 Example 61. benzene 37 Example 62. chloroform 39 Example 63. Ethanol 45

Example 64-75.

The solvent of Table 7 was used and extracted in the same manner as in Example 30, and the results are shown in Table 7 using lactose as an excipient.

TABLE 7

Extraction solvent Dry weight of the final extract [g] Example 65. water 44 Example 66. ethanol 48 Example 67. Methanol 43 Example 68. Propanol 43 Example 69. Butanol 42 Example 70. Acetone 43 Example 71. Ethyl acetate 38 Example 72. Hexane 37 Example 73. benzene 37 Example 74. chloroform 35 Example 75. Ethanol 45

Examples 76-86.

The solvent of Table 8 was used and extracted in the same manner as in Example 30, and the results are shown in Table 8 using silica powder as an excipient.

TABLE 8

Extraction solvent Dry weight of the final extract [g] Example 76. water 42 Example 77. ethanol 43 Example 78. Methanol 44 Example 79. Propanol 42 Example 80. Butanol 46 Example 81. Acetone 43 Example 82. Ethyl acetate 39 Example 83. Hexane 40 Example 84. benzene 38 Example 85. chloroform 38 Example 86. Ethanol 45

Experiment 1. Cytotoxicity Test

In order to examine the cytotoxicity of the extract, cultured V79-4 cells [pulmonary tissue fibroblasts of CCL (continuous cell line), MTT [3- (4,5-dimethyltazol-2] -Yl) -2,5-diphenyltetrazolium bromide reduction test] and the MTT-formazan product was extracted with isopropyl alcohol to measure absorbance at 570 nm. In order to know the IC50 (inhibition concentration 50) for each substance, the substance was diluted in 10.0% and 1.0% in medium and incubated for 24 hours to obtain the IC50.

[Table 9] Cytotoxicity test results of March extract

sample IC50 (%, W / V) SLS 0.0025 Example 1. > 5.0 Example 2. > 5.0 Example 3. > 5.0 Example 4. > 5.0 Example 5. > 5.0 Example 6. > 5.0 Example 7. > 5.0 Example 8. > 5.0 Example 9. > 5.0 Example 10. > 5.0 Example 11. > 5.0 Example 12. > 5.0 Example 13. > 5.0 Example 14. > 5.0 Example 15. > 5.0 Example 16. > 5.0 Example 17. > 5.0 Example 18. > 5.0

Experiment 2. Cytotoxicity Test of Powdered Samples

0.3 g of powdered extract was dissolved in 100 g of purified water, and was treated in the same manner as in Experiment 1. The results of obtaining the IC 50 are shown in Table 10.

[Table 10] Cytotoxicity test results of March extract

sample IC50 (%, W / V) Example 19. > 5.0 Example 20. > 5.0 Example 21. > 5.0 Example 22. > 5.0 Example 23. > 5.0 Example 24. > 5.0 Example 25. > 5.0 Example 26. > 5.0 Example 27. > 5.0 Example 28. > 5.0 Example 29. > 5.0

Experiment 3. Cytotoxicity Test of Powdered Samples

0.9 g of powdered extract was dissolved in 100 g of purified water and tested in the same manner as in Experiment 1. The results of obtaining the IC 50 are shown in Table 11.

[Table 11] Cytotoxicity test results of Machi Prefecture extract

sample IC50 (%, W / V) Example 30. > 5.0 Example 31. > 5.0 Example 32. > 5.0 Example 33. > 5.0 Example 34. > 5.0 Example 35. > 5.0 Example 36. > 5.0 Example 37. > 5.0 Example 38. > 5.0 Example 39. > 5.0 Example 40. > 5.0 Example 41. > 5.0 Example 42. > 5.0 Example 43. > 5.0 Example 44. > 5.0 Example 45. > 5.0 Example 46. > 5.0 Example 47. > 5.0 Example 48. > 5.0 Example 49. > 5.0 Example 50. > 5.0 Example 60. > 5.0 Example 61. > 5.0 Example 62. > 5.0 Example 63. > 5.0 Example 64. > 5.0 Example 65. > 5.0 Example 66. > 5.0 Example 67. > 5.0 Example 68. > 5.0 Example 69. > 5.0 Example 70. > 5.0 Example 71. > 5.0 Example 72. > 5.0 Example 73. > 5.0 Example 74. > 5.0 Example 75. > 5.0

sample IC50 (%, W / V) Example 76. > 5.0 Example 77. > 5.0 Example 78. > 5.0 Example 79. > 5.0 Example 80. > 5.0 Example 81. > 5.0 Example 82. > 5.0 Example 83. > 5.0 Example 84. > 5.0 Example 85. > 5.0 Example 86. > 5.0

As shown in Tables 9, 10 and 11, the gusset extract was more than 2,000 times lower in cytotoxicity than sodium lauryl sulfate, and thus, it was confirmed that the extract was excellent in safety.

Experiment 4. Allergy assessment

To confirm the allergy to the extract, apply 25 μl of the sample to the mouse ear once a day for 3 days, remove the lymph node on the 4th day, isolate the lymphocytes, and incubate for 24-48 hours in a 5% carbon dioxide incubator. The amount of radioisotope [3 H] -methylthymidine is measured. The local lymph node assay (LLNA) results indicate the lymphocyte amplification of the sample group compared to the control group, and allergens when the amplification degree (SI) of the sample increases more than three times and increases with each concentration even at one concentration. Considered as.

[Table 12] Allergy Evaluation Results of Machi Prefecture Extracts

sample density(%) Average cpm Amplification degree (S.I) ethanol 60 2261 ± 121 - Example 1 Extract 100 4548 ± 331 2.01 Example 2 Extract 100 3187 ± 288 1.41 Example 3 Extract 100 2993 ± 231 1.32 Example 4 Extract 100 3233 ± 265 1.43 Example 5 Extract 100 4533 ± 215 2.00 Example 6 Extract 100 4238 ± 241 1.87 Example 7 Extract 100 3215 ± 125 1.42 Example 8 Extract 100 4522 ± 135 2.00 Example 9 Extract 100 4002 ± 195 1.77 Example 10 Extract 100 4566 ± 301 1.58 Example 11 Extract 100 4577 ± 302 2.02 Example 12 Extract 100 4321 ± 125 1.91 Example 13 Extract 100 4110 ± 145 1.82 Example 14 Extract 100 3993 ± 187 1.77 Example 15 Extract 100 4325 ± 168 1.91 Example 16 Extract 100 3200 ± 188 1.42 Example 17 Extract 100 4555 ± 136 2.01 Example 18 Extract 100 4633 ± 205 2.05

Cpm (counter per minute): The amount of [3H] -methylthymidine inserted into the cell.

S.I (stimulation index): Average cpm of sample divided by average cpm of control

Experiment 5. Allergy Assessment of Powdered Samples

0.3 g of powdered extract was dissolved in 100 g of purified water, and was treated in the same manner as in Experiment 4. The results are shown in Table 13.

[Table 13] Allergy Evaluation Results of Machi Prefecture Extracts

sample density(%) Average cpm Amplification degree (S.I) Example 19 Extract 100 3878 ± 231 1.72 Example 20 Extract 100 4655 ± 254 2.06 Example 21 Extract 100 3322 ± 310 1.47 Example 22 Extract 100 3221 ± 241 1.42 Example 23 Extract 100 3845 ± 284 1.70 Example 24 Extract 100 3955 ± 262 1.75 Example 25 Extract 100 3785 ± 162 1.67 Example 26 Extract 100 4121 ± 198 1.82 Example 27 Extract 100 4222 ± 199 1.87 Example 28 Extract 100 4321 ± 173 1.91 Example 29 Extract 100 4563 ± 123 2.02

Experiment 6. Allergy Assessment of Powdered Samples

0.9 g of powdered extract was dissolved in 100 g of purified water, and was treated in the same manner as in Experiment 4. The results are shown in Table 14.

[Table 14] Allergy Evaluation Results of Machi Prefecture Extracts

sample density(%) Average cpm Amplification degree (S.I) Example 30 Extract 100 4213 ± 213 1.86 Example 31 Extract 100 4333 ± 255 1.92 Example 32 Extract 100 3566 ± 271 1.58 Example 33 Extract 100 4566 ± 162 2.02 Example 34 Extract 100 4523 ± 144 2.00 Example 35 Extract 100 4822 ± 132 2.13 Example 36 Extract 100 4321 ± 115 1.91 Example 37 Extract 100 4888 ± 132 2.16 Example 38 Extract 100 4354 ± 123 1.93 Example 39 Extract 100 4666 ± 145 2.06 Example 40 Extract 100 4822 ± 122 2.13 Example 41 Extract 100 4211 ± 158 1.86 Example 42 Extract 100 4333 ± 184 1.92 Example 43 Extract 100 4111 ± 188 1.82 Example 44 Extract 100 3555 ± 165 1.57 Example 45 Extract 100 4355 ± 154 1.93 Example 46 Extract 100 4123 ± 156 1.82 Example 47 Extract 100 3212 ± 275 1.42 Example 48 Extract 100 3666 ± 266 1.62 Example 49 Extract 100 3212 ± 234 1.42 Example 50 Extract 100 4232 ± 201 1.87 Example 51 Extract 100 4551 ± 187 2.01

sample density(%) Average cpm Amplification degree (S.I) Example 52 Extract 100 3845 ± 195 1.70 Example 53 Extract 100 3645 ± 235 1.61 Example 54 Extract 100 4123 ± 213 1.82 Example 55 Extract 100 4236 ± 272 1.87 Example 56 Extract 100 4875 ± 274 2.16 Example 57 extract 100 3212 ± 266 1.42 Example 58 Extract 100 3322 ± 254 1.47 Example 59 Extract 100 3456 ± 278 1.53 Example 60 Extract 100 3812 ± 234 1.69 Example 61 extract 100 4102 ± 108 1.81 Example 62 Extract 100 3205 ± 192 1.42 Example 63 Extract 100 3653 ± 162 1.62 Example 64 Extract 100 3858 ± 172 1.71 Example 65 Extract 100 3566 ± 176 1.58 Example 66 extract 100 4062 ± 135 1.80 Example 67 Extract 100 4744 ± 205 2.10 Example 68 Extract 100 4953 ± 103 2.19 Example 69 Extract 100 3645 ± 135 1.61 Example 70 Extract 100 3126 ± 198 1.38 Example 71 Extract 100 4321 ± 128 1.91 Example 72 Extract 100 4333 ± 166 1.92 Example 73 Extract 100 4122 ± 175 1.82 Example 74 Extract 100 4621 ± 158 2.04 Example 75 Extract 100 3585 ± 152 1.59 Example 76 Extract 100 3956 ± 192 1.75 Example 77 Extract 100 3753 ± 152 1.66 Example 78 Extract 100 3785 ± 184 1.67 Example 79 Extract 100 3745 ± 192 1.66 Example 80 Extract 100 3651 ± 143 1.61 Example 81 Extract 100 3555 ± 183 1.57 Example 82 Extract 100 3961 ± 174 1.75 Example 83 Extract 100 3650 ± 185 1.61 Example 84 Extract 100 3905 ± 186 1.73 Example 85 Extract 100 3681 ± 162 1.63 Example 86 Extract 100 3654 ± 182 1.62

As shown in Tables 12, 13, and 14, the amplification degree (S.I) of the extract was found to be less than 3 allergic potential.

Experiment 7. Anti-inflammatory experiment

In order to examine the anti-inflammatory effects on the extract, the left ear of the mouse was used as the control site and the right ear as the test site. One hour after the last application, ethanol was applied to the left ear and arachidonic acid (2 mg / ear) was applied to the right ear. After 1 hour, the ear edema was measured three times by micrometer.

Anti-inflammatory effect was determined as the degree of edema inhibition based on the arachidonic acid treatment group and the results are shown in Table 15.

[Table 15] Ear thickness and inflammation inhibition rate by topical application

sample density(%) menstruum Ear thickness (㎛) % Inhibition Before sample processing After sample processing Indomethacin 1.0 ethanol 284 421 45.6 Arachidonic acid 2mg / ear ethanol 299 551 - Example 1 Extract 0.5 ethanol 288 499 16.3 Example 1 Extract 1.0 ethanol 279 477 21.4 Example 2 Extract 0.5 ethanol 279 492 15.5 Example 2 Extract 1.0 ethanol 296 497 20.2 Example 2 Extract 2.0 ethanol 293 478 26.6 Example 3 Extract 0.5 ethanol 295 511 14.3 Example 3 Extract 2.0 ethanol 283 472 25.0 Example 4 Extract 1.0 ethanol 284 487 19.3 Example 5 Extract 1.0 ethanol 263 466 19.5 Example 6 Extract 1.0 ethanol 279 495 14.3 Example 7 Extract 1.0 ethanol 288 499 16.4 Example 8 Extract 1.0 ethanol 284 505 12.4 Example 9 Extract 1.0 ethanol 294 511 13.8 Example 10 Extract 1.0 ethanol 295 498 19.6 Example 11 Extract 1.0 ethanol 293 506 15.3 Example 12 Extract 1.0 ethanol 295 503 17.4 Example 13 Extract 1.0 ethanol 287 495 17.4 Example 14 Extract 1.0 ethanol 276 479 19.5 Example 15 Extract 1.0 ethanol 271 474 19.5 Example 16 Extract 1.0 ethanol 285 489 18.9 Example 17 Extract 1.0 ethanol 296 503 17.8 Example 18 Extract 1.0 ethanol 275 477 19.9

※ Inhibition Rate (%) = (A―B) / A × 100

A: Average thickness of control ears (thickness of arachidonic acid treated ears-thickness of untreated ears)

B: thickness of the sample-coating group ear (thickness of the sample-treated ear-thickness of the untreated ear)

Experiment 8. Anti-inflammatory Experiment of Powdered Sample

0.3 g of powdered extract was dissolved in 100 g of purified water, and was carried out in the same manner as in Experiment 5. The results are shown in Table 16.

[Table 16] Ear thickness and inflammation inhibition rate by topical application

sample density(%) menstruum Ear thickness (㎛) % Inhibition Before sample processing After sample processing Example 19 Extract 1.0 ethanol 263 472 17.2 Example 20 Extract 1.0 ethanol 278 501 11.4 Example 21 Extract 1.0 ethanol 298 502 19.2 Example 22 Extract 1.0 ethanol 263 471 17.4 Example 23 Extract 1.0 ethanol 287 501 15.0 Example 24 Extract 1.0 ethanol 289 500 16.4 Example 25 Extract 1.0 ethanol 278 486 17.5 Example 27 Extract 1.0 ethanol 274 483 17.1 Example 28 Extract 1.0 ethanol 273 486 15.3 Example 29 Extract 1.0 ethanol 271 477 18.1

Experiment 9. Anti-inflammatory Experiment of Powdered Sample

0.9 g of powdered extract was dissolved in 100 g of purified water, and was treated in the same manner as in Experiment 5. The results are shown in Table 17.

Table 17. Ear thickness and inflammation inhibition rate by topical application

sample density(%) menstruum Ear thickness (㎛) % Inhibition Before sample processing After sample processing Example 30 Extract 1.0 ethanol 280 488 17.4 Example 31 Extract 1.0 ethanol 295 506 16.4 Example 32 Extract 1.0 ethanol 276 479 19.4 Example 33 Extract 1.0 ethanol 275 486 16.4 Example 34 Extract 1.0 ethanol 288 496 17.4 Example 35 Extract 1.0 ethanol 293 501 17.4 Example 36 Extract 1.0 ethanol 277 480 19.3 Example 37 Extract 1.0 ethanol 275 482 17.7 Example 38 Extract 1.0 ethanol 285 488 19.6 Example 39 Extract 1.0 ethanol 265 473 17.3 Example 40 Extract 1.0 ethanol 275 483 17.4 Example 41 Extract 1.0 ethanol 277 484 17.8 Example 42 Extract 1.0 ethanol 287 490 19.5 Example 43 Extract 1.0 ethanol 288 499 16.3 Example 44 Extract 1.0 ethanol 279 477 21.4 Example 45 Extract 1.0 ethanol 279 492 15.5 Example 46 Extract 1.0 ethanol 296 497 20.2 Example 47 Extract 1.0 ethanol 293 478 26.6 Example 48 Extract 1.0 ethanol 295 511 14.3 Example 49 Extract 1.0 ethanol 283 472 25.0 Example 50 Extract 1.0 ethanol 284 475 24.3 Example 51 Extract 1.0 ethanol 273 487 15.0 Example 52 Extract 1.0 ethanol 288 498 16.6 Example 53 Extract 1.0 ethanol 274 490 14.3 Example 54 Extract 1.0 ethanol 275 475 20.5 Example 55 Extract 1.0 ethanol 293 491 21.3 Example 56 Extract 1.0 ethanol 285 498 15.6 Example 57 extract 1.0 ethanol 296 500 18.69 Example 58 Extract 1.0 ethanol 274 487 15.3 Example 59 Extract 1.0 ethanol 282 492 16.6 Example 60 Extract 1.0 ethanol 283 493 16.5 Example 61 extract 1.0 ethanol 288 510 12.0 Example 62 Extract 1.0 ethanol 274 482 17.6 Example 63 Extract 1.0 ethanol 295 500 18.6 Example 64 Extract 1.0 ethanol 274 477 19.5 Example 65 Extract 1.0 ethanol 275 476 20.2 Example 66 extract 1.0 ethanol 276 493 13.8 Example 67 Extract 1.0 ethanol 284 499 14.6 Example 68 Extract 1.0 ethanol 297 505 17.6 Example 69 Extract 1.0 ethanol 277 490 15.3 Example 70 Extract 1.0 ethanol 270 481 16.3 Example 71 Extract 1.0 ethanol 280 490 16.5 Example 72 Extract 1.0 ethanol 285 499 15.1 Example 73 Extract 1.0 ethanol 290 497 17.8

sample density(%) menstruum Ear thickness (㎛) % Inhibition Before sample processing After sample processing Example 74 Extract 1.0 ethanol 280 488 17.6 Example 75 Extract 1.0 ethanol 277 485 17.3 Example 76 Extract 1.0 ethanol 279 489 16.5 Example 77 Extract 1.0 ethanol 287 489 19.8 Example 78 Extract 1.0 ethanol 284 497 15.5 Example 79 Extract 1.0 ethanol 286 504 13.4 Example 80 Extract 1.0 ethanol 287 488 20.1 Example 81 Extract 1.0 ethanol 295 500 18.8 Example 82 Extract 1.0 ethanol 276 486 16.6 Example 83 Extract 1.0 ethanol 276 486 16.5 Example 84 Extract 1.0 ethanol 284 493 17.2 Example 85 Extract 1.0 ethanol 298 506 17.5 Example 86 Extract 1.0 ethanol 274 477 19.6

As shown in Tables 15, 16, and 17, the gut extract showed high anti-inflammatory inhibition rate, and the machi extract showed a 99% significance level with arachidonic acid.

Experiment 10. Skin primary irritation test (closed patch test)

After extracting 1.0%, 3.0% aqueous solution and 0.08% aqueous solution of sodium lauryl sulfate in a healthy adult male and female, a certain amount (0.2 g) of each sample was applied to the back area for 24 hours, and then the pin chamber was removed. After the passage of time, the skin condition was visually read and the results are shown in Table 18.

[Table 18] Result of primary skin irritation test

sample Number of test subjects Judgment result Stimulus ++ + +- - Sodium Lauryl Sulfate 0.08% 50 One 3 13 33 0.40 Purified water 50 0 0 6 44 0.12 Example 1 Extract 1.0% + Sodium Lauryl Sulfate 0.08% 50 0 0 9 41 0.18 Example 2 3.0% extract + sodium lauryl sulfate 0.08% 50 0 0 6 44 0.12 Example 3 3.0% extract + sodium lauryl sulfate 0.08% 50 One 3 13 33 0.44 Example 4 Extract 1.0% + Sodium Lauryl Sulfate 0.08% 50 0 0 6 44 0.12 Example 5 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 One 0 5 44 0.16 Example 6 3.0% extract + sodium lauryl sulfate 0.08% 50 0 0 3 47 0.06 Example 7 Extract 1.0% + Sodium Lauryl Sulfate 0.08% 50 0 2 8 40 0.24 Example 8 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 0 0 5 45 0.1 Example 9 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 0 3 6 41 0.24 Example 10 Extract 1.0% + Sodium Lauryl Sulfate 0.08% 50 One 2 4 43 0.22 Example 11 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 0 One 5 44 0.14 Example 12 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 0 0 6 44 0.12 Example 13 Extract 1.0% + Sodium Lauryl Sulfate 0.08% 50 0 0 2 48 0.04 Example 14 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 0 0 4 46 0.08 Example 15 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 0 0 5 45 0.1 Example 16 extract 1.0% + sodium lauryl sulfate 0.08% 50 One 2 8 39 0.3 Example 17 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 One 0 9 40 0.24 Example 18 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 0 One 7 42 0.18

※ Criteria - ; No erythema or unusual symptoms

+-; Slightly redder than the surroundings

+; Significantly redder than the surroundings

++; Severe redness and swelling than the surroundings

{(+-) Number × 1} + {(+) number × 02} + {(++) number × 3}

Stimulation degree = -----------------------------------------

Number of test subjects

Experiment 11. Primary skin irritation test of powdered sample (closed patch test)

0.3 g of the powdered extract was dissolved in 100 g of purified water, and was carried out in the same manner as in Experiment 5. The results are shown in Table 19.

[Table 19] Result of primary skin irritation test

sample Number of test subjects Judgment result Stimulus ++ + +- - Example 19 Extract 1.0% + Sodium Lauryl Sulfate 0.08% 50 0 0 6 44 0.12 Example 20 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 0 0 4 46 0.08 Example 21 Extract 1.0% + Sodium Lauryl Sulfate 0.08% 50 0 3 5 42 0.22 Example 22 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 0 0 4 46 0.08 Example 23 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 0 0 5 45 0.10 Example 24 Extract 1.0% + Sodium Lauryl Sulfate 0.08% 50 0 0 4 46 0.08 Example 25 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 0 2 4 44 0.16 Example 26 extract 3.0% + sodium lauryl sulfate 0.08% 50 0 0 6 44 0.12 Example 27 Extract 1.0% + Sodium Lauryl Sulfate 0.08% 50 0 0 6 44 0.12 Example 28 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 0 One 8 41 0.20 Example 29 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 0 0 4 46 0.08

Experiment 12. Primary skin irritation test of powdered sample (closed patch test)

0.9 g of powdered extract was dissolved in 100 g of purified water, and was carried out in the same manner as in Experiment 5. The results are shown in Table 20.

Table 20 Skin primary irritation test results

sample Number of test subjects Judgment result Stimulus ++ + +- - Example 30 Extract 1.0% + Sodium Lauryl Sulfate 0.08% 50 0 One 5 44 0.14 Example 31 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 0 0 6 44 0.12 Example 32 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 0 0 8 42 0.16 Example 33 Extract 1.0% + Sodium Lauryl Sulfate 0.08% 50 0 One 7 42 0.18 Example 34 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 0 0 3 47 0.06 Example 35 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 0 0 6 44 0.12 Example 36 extract 1.0% + sodium lauryl sulfate 0.08% 50 0 0 8 41 0.22 Example 37 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 0 0 4 46 0.08 Example 38 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 0 0 5 45 0.10 Example 39 extract 1.0% + sodium lauryl sulfate 0.08% 50 0 0 6 44 0.12 Example 40 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 0 One 8 41 0.20

sample Number of test subjects Judgment result Stimulus ++ + +- - Example 41 Extract 1.0% + Sodium Lauryl Sulfate 0.08% 50 One 0 4 45 0.14 Example 42 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 0 One 12 37 0.28 Example 43 Extract 1.0% + Sodium Lauryl Sulfate 0.08% 50 0 0 6 44 0.12 Example 44 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 One One 5 43 0.20 Example 45 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 0 0 5 45 0.10 Example 46 Extract 1.0% + Sodium Lauryl Sulfate 0.08% 50 0 0 6 44 0.12 Example 47 extract 3.0% + sodium lauryl sulfate 0.08% 50 One 0 4 45 0.14 Example 48 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 0 0 5 45 0.10 Example 49 Extract 1.0% + Sodium Lauryl Sulfate 0.08% 50 0 0 6 44 0.12 Example 50 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 One One 7 41 0.24 Example 51 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 0 0 5 45 0.10 Example 52 Extract 1.0% + Sodium Lauryl Sulfate 0.08% 50 0 0 6 44 0.12 Example 53 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 0 0 4 46 0.08 Example 54 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 0 0 8 42 0.16 Example 55 Extract 1.0% + Sodium Lauryl Sulfate 0.08% 50 0 One 5 44 0.14 Example 56 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 0 0 9 41 0.18 Example 57 extract 3.0% + sodium lauryl sulfate 0.08% 50 0 0 6 44 0.12 Example 58 Extract 1.0% + Sodium Lauryl Sulfate 0.08% 50 0 0 5 45 0.10 Example 59 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 0 One One 48 0.06 Example 60 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 0 0 5 45 0.10 Example 61 extract 1.0% + sodium lauryl sulfate 0.08% 50 0 0 4 46 0.08 Example 62 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 0 2 9 39 0.26 Example 63 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 0 0 8 42 0.16 Example 64 Extract 1.0% + Sodium Lauryl Sulfate 0.08% 50 One 2 7 40 0.28 Example 65 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 0 0 9 41 0.18 Example 66 extract 3.0% + sodium lauryl sulfate 0.08% 50 0 0 2 48 0.04 Example 67 Extract 1.0% + Sodium Lauryl Sulfate 0.08% 50 0 0 One 49 0.02 Example 68 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 One 0 2 47 0.10 Example 69 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 0 2 3 45 0.14 Example 70 Extract 1.0% + Sodium Lauryl Sulfate 0.08% 50 0 0 One 49 0.02 Example 71 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 0 0 3 47 0.03 Example 72 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 0 2 One 47 0.10 Example 73 Extract 1.0% + Sodium Lauryl Sulfate 0.08% 50 0 0 3 47 0.06 Example 74 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 0 0 5 45 0.10 Example 75 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 0 0 6 44 0.12 Example 76 Extract 1.0% + Sodium Lauryl Sulfate 0.08% 50 0 0 4 46 0.08 Example 77 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 0 2 5 43 0.18 Example 78 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 0 0 6 44 0.12 Example 79 Extract 1.0% + Sodium Lauryl Sulfate 0.08% 50 0 0 5 45 0.10 Example 80 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 0 2 4 44 0.16 Example 81 Extract 1.0% + Sodium Lauryl Sulfate 0.08% 50 0 0 6 44 0.12 Example 82 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 0 0 8 42 0.16 Example 83 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 0 One 5 44 0.14 Example 84 Extract 1.0% + Sodium Lauryl Sulfate 0.08% 50 0 0 3 47 0.06 Example 85 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 0 0 One 49 0.02 Example 86 Extract 3.0% + Sodium Lauryl Sulfate 0.08% 50 0 0 2 48 0.04

Experiment 13. Antibacterial test

Antimicrobial experiments were measured by agar diffusion using a paper disk. Inoculate strains into 100 ml of liquid medium and incubate them for 18 to 24 hours at red = = constant temperature to activate them. The solution was inoculated into the top agar (0.75% agar) by 100 µl (107-108 cfu / ml) and covered on the plate. Each extract was dried on sterile disks and dried on 20 µl. After 36 hours of incubation, the antimicrobial activity was measured by the diameter of the inhibitory rings formed around the disc (mm).

Use strain and medium

It was used as the microbial test strain as follows. Escherichia Coli, Staphylococcus aureus, Bacillus subtilis, and candida albican were selected. The growth medium of the bacteria was used Trypticase soy agar (Difco).

The experimental results are shown in Table 21.

[Table 21] Antibacterial test results

sample Esherichia Coli Staphylococcus aureus Bacillus subtilis Candida albican Example 1 Extract 9 10 10 8 Example 2 Extract 9 10 10 8 Example 3 Extract 8 9 9 8 Example 4 Extract 9 10 10 9 Example 5 Extract 9 9 9 9 Example 6 Extract 10 9 8 8 Example 7 Extract 9 9 8 8 Example 8 Extract 9 10 10 9 Example 9 Extract 9 9 8 9 Example 10 Extract 9 8 8 9 Example 11 Extract 10 10 9 9 Example 12 Extract 8 9 8 9 Example 13 Extract 8 9 8 9 Example 14 Extract 8 10 10 10 Example 15 Extract 9 9 11 9 Example 16 Extract 9 8 11 9 Example 17 Extract 9 10 10 9 Example 18 Extract 9 9 11 9

Experiment 14. Antibacterial Test of Powdered Samples

1.0 g of powdered extract was dissolved in 99 g of purified water, and the experiment was performed in the same manner as in Experiment 14. The results are shown in Table 22.

[Table 22] Antibacterial test results of powdered samples

sample Escherichia Coli Staphylococcus aureus Bacillus subtilis Candida albican Example 19 Extract 9 11 8 11 Example 20 Extract 10 11 9 10 Example 21 Extract 10 10 8 8 Example 22 Extract 8 10 9 11 Example 23 Extract 8 8 10 9 Example 24 Extract 9 10 9 9 Example 25 Extract 10 9 8 11 Example 26 Extract 9 11 8 8 Example 27 Extract 9 11 10 9 Example 28 Extract 10 9 10 9 Example 29 Extract 9 8 8 11

Experiment 15. Antibacterial Test of Powdered Samples

2.0 g of powdered extract was dissolved in 98 g of purified water, and was carried out in the same manner as in Experiment 14. The experimental results are shown in Table 23.

[Table 23] Antibacterial test results of powdered samples

sample Esherichia Coli Staphylococcus aureus Bacillus subtilis Candida albican Example 30 Extract 9 8 9 9 Example 31 Extract 8 10 9 8 Example 32 Extract 8 10 9 10 Example 33 Extract 8 8 10 9 Example 34 Extract 8 10 9 9 Example 35 Extract 8 9 8 9 Example 36 Extract 9 10 8 9

sample Esherichia Coli Staphylococcus aureus Bacillus subtilis Candida albican Example 37 Extract 8 9 10 9 Example 38 Extract 9 9 10 9 Example 39 Extract 9 8 8 9 Example 40 Extract 10 8 9 10 Example 41 Extract 9 10 8 8 Example 42 Extract 8 10 9 8 Example 43 Extract 8 8 10 9 Example 44 Extract 9 10 9 9 Example 45 Extract 10 9 8 8 Example 46 Extract 9 8 8 8 Example 47 Extract 9 8 10 9 Example 48 Extract 10 9 10 9 Example 49 Extract 9 8 8 9 Example 50 Extract 10 10 9 9 Example 51 Extract 9 9 9 8 Example 52 Extract 8 8 9 8 Example 53 Extract 9 9 10 10 Example 54 Extract 9 10 9 9 Example 55 Extract 10 9 8 8 Example 56 Extract 9 8 8 8 Example 57 extract 9 8 9 10 Example 58 Extract 9 9 8 9 Example 59 Extract 9 10 8 9 Example 60 Extract 10 9 8 10 Example 61 extract 9 9 8 8 Example 62 Extract 8 8 10 8 Example 63 Extract 9 8 10 9 Example 64 Extract 9 10 10 9 65 extracts in the embodiment 8 9 8 8 Example 66 extract 9 9 8 8 Example 67 Extract 9 8 10 9 Example 68 Extract 9 9 8 9 Example 69 Extract 9 8 10 9 Example 70 Extract 9 9 9 9 Example 71 Extract 9 10 8 8 Example 72 Extract 8 10 9 8 Example 73 Extract 10 8 10 9 Example 74 Extract 9 9 9 9 Example 75 Extract 10 9 8 8 Example 76 Extract 9 9 8 8 Example 77 Extract 8 8 10 9 Example 78 Extract 9 10 8 9 Example 79 Extract 10 9 8 9 Example 80 Extract 10 8 9 9 Example 81 Extract 9 10 8 8 Example 82 Extract 8 10 9 8 Example 83 Extract 8 8 10 9 Example 84 Extract 8 10 9 9 Example 85 Castings 10 10 9 8 Example 86 Extract 9 8 8 8 Example 87 Extract 9 8 8 9

Application Example 1. Treatment of upper sheet using gusset extract

The gusset extract extracted by the method of Example 1-18 was coated with a polyolefin (eg polypropylene) fiber at about 5-50% by weight to prepare a nonwoven fabric coated with a gusset through a carding and heat bonding process.

Application Example 2 Treatment of Top Sheet Using Powdery Macchi Extract

An acidic aqueous solution was prepared in 5% (w / w) by using an aqueous solution containing 1.5% (w / w) of acetic acid, and the powdery gusset extract extracted by the method of Example 19-87 was used for the polyolefin fiber. After coating at 5-50% by weight, a non-woven fabric was coated with a gusset string through a carding and heat bonding process.

Application Example 3. Preparation of Absorbent Using Machi Prefecture Extract

An extract was prepared by mixing 1-50% by weight of the Portuguese extract extracted by the method of Example 1-18 with respect to the weight of the wood pulp.

Application Example 4. Preparation of absorber using powdery gusset extract

A powdery gusset extract extracted by the method of Example 19-87 was mixed at a weight of 1-50% with respect to the weight of the wood pulp to prepare an absorbent body.

Application Example 5. Fabrication of Disposable Diapers

Disposable diapers according to the present invention were produced by a conventional diaper manufacturing process using the top sheet and / or absorber prepared by the method of Application Examples 1-4.

Disposable diaper coating composition of the present invention contains an anti-allergic, anti-inflammatory and antimicrobial activity of Machi Prefecture exhibits anti-allergic, anti-inflammatory and antimicrobial activity on the skin.

Claims (6)

Disposable Diaper Coating Composition Containing Machi (Sorbus) Extract The method according to claim 1, wherein the extract of Machi Prefecture is water, ethanol, methanol, propanol, butanol, acetone, ethyl acetate, hexane, benzene, chloroform, glycerin, butylene glycol, propylene glycol, hydrous ethanol, hydrous methanol, hydrous propanol , Extract one or two or more selected from the group consisting of hydrous butanol, hydrous glycerin, hydrous butylene glycol and hydrous propylene glycol, and extract the extract or its powder extracted at 95 ° C. for 4-20 hours or at 5-40 ° C. for 1-15 days. Disposable diaper coating composition comprising The composition of claim 1 or 2, wherein the composition further comprises one or more excipients selected from the group consisting of β-CD (cyclodextrin), hydroxypropyl-β-CD, malto-dextrin, lactose, and silica powder. Disposable diaper coating composition comprising a. A polyolefin nonwoven fabric for disposable diaper upper sheets, characterized by coating Machi Prefecture extract. Pulp absorbent for disposable diapers, characterized in that the extract of Machi. Disposable diaper, characterized in that the extract of Machi.