KR20030005720A - A cosmetic composition containing an extract of ampelopsis japonica - Google Patents

Abstract

PURPOSE: A cosmetic composition containing an Ampelopsis japonica extract exhibiting a skin whitening effect, skin irritation reducing effect and immunostimulating effect as a main component is provided. The extract has a cell membrane binding inhibiting effect of MSH for protecting melanin formation, human tyrosinase activity inhibiting effect, tyrosinase-synthesis inhibiting effect, melanin-production inhibiting effect and cell death inhibiting effect, and inflammation-inducing enzyme activity inhibiting effect, it can be thus used as a cosmetic having the above effect. CONSTITUTION: The skin whitening cosmetic composition contains 0.01 to 30% by weight of an Ampelopsis japonica extract, based on the total weight of the composition. The extract is prepared by extracting Ampelopsis japonica(Thunb.) Makino in one or more solvents selected from purified water, lower alcohol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloro methane and hexane, filtrating and concentrating or freeze-drying.

Description

Cosmetic composition containing the extract of baekryeom {A COSMETIC COMPOSITION CONTAINING AN EXTRACT OF AMPELOPSIS JAPONICA}

The present invention relates to a skin cosmetic containing an active ingredient extracted from baekryeom.

Recently, many cosmetic products using natural products have been developed to reduce skin irritation. Various herbal raw materials are extracted by a predetermined method, and the function of the extracts is being increased to develop various functional cosmetics.

For example, mung beans have a known function to clean the skin, and other ginseng extracts, mushroom extracts, etc., have been found to have unique anti-aging or whitening effects and are applied to various cosmetic products.

These natural ingredients have less side effects on the skin, and as the consumer's response to cosmetics using natural materials has recently increased, the development value of cosmetics as a raw material for cosmetics is further increased.

Accordingly, the present inventors have studied the applicability to cosmetics in other herbal ingredients that have not been known so far, it has been found that the extract of baekryeom than the expected efficacy as a cosmetic such as whitening effect.

White leaf is a deciduous broad-leaved vine of grape family, also known as salt, white vine (白草). It is a perennial plant that grows in Korea, China, and Japan. It extends more than 2m in length and has a reddish lump root. Flowers are bisexual, inflorescences of inflorescences, pale yellow, and bloom in June-July. Fruits ripen in September-October with berries. In oriental medicine, the roots are taken in the fall, peeled, sliced, and sun-dried, called pneumonia or white root. It is prescribed for children's games, tuberculous lymphadenitis, endometritis, enteritis, dental fever, burns, and venom. Ingredients include mucin, starch, ampelopsin, slime, and starch. The scientific name is Amelopsis japonica (Han Dae-seok, Pharmacology, Dong-myung, 1998).

An example of applying such baekryeom as a cosmetic is disclosed in Korean Patent Application No. 98-49138. However, the extract disclosed in this application is a combination of baekryeomhwa, white paper, gobon, cheongung, apricot seed, golden, donkey, mung bean, three hundred seconds, ginseng leaf, rich, tofu, windproof, shiitake mushrooms 1,3 It is extracted with butylene glycol, and already contains well-known raw materials such as a whitening effect such as mung beans and apricot seeds, and the efficacy of the extract extracted only from baekrye is not disclosed.

Accordingly, the present inventors select baekryeom among various herbal raw materials to confirm its efficacy as a cosmetic raw material and develop a method of using the same to increase the selection range of various cosmetic raw materials, and also use this raw material without any stimulus, It is intended to provide a method for producing a cosmetic exhibiting a functional effect of.

In addition, from the point of view that the target efficacy was more greatly selected when selecting a specific extraction solvent when extracting baekryeom, to provide a method for producing a cosmetic using these materials as a cosmetic raw material and the cosmetic.

In order to achieve the above object, the present invention provides a cosmetic composition for skin whitening containing baekryeom extract as the main active ingredient.

In addition, the present invention is characterized by using at least one solvent selected from purified water, lower alcohol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane and hexane as the extract.

In addition, the present invention is characterized in that the extraction using the ethanol 40-95% solution as the extraction solvent.

In addition, the present invention is characterized in that the extraction is carried out by a cooling or heating method.

Furthermore, the present invention is characterized by further performing filtration, concentration or lyophilization after extraction.

In addition, the cosmetic of the present invention is characterized by containing 0.01 to 30% by weight of baekryeom extract.

In addition, the cosmetic composition of the present invention is characterized in that one of the formulation of a lotion, gel, water-soluble liquid, oil-in-water (O / W) type and water-in-oil (W / O) type.

The cosmetic composition is characterized in that it is used for skin whitening.

The cosmetic composition is characterized in that it is used for skin irritation relief.

The cosmetic composition is characterized in that it is used for skin immunity enhancement.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, these Examples are only illustrative description of the present invention and the scope of the present invention is not limited to these Examples.

Example 1 Preparation of Extract

The dry and white pneumonia was chopped and refluxed three times for 5 hours with a hot 95% (V / V) ethanol aqueous solution, and then cooled and filtered through Whatman # 5 filter paper. The filtered extract was concentrated under reduced pressure and lyophilized at 50 ° C or lower. The pneumonia extract was prepared using one or more solvents selected from purified water, ethanol, butylene glycol, and propylene glycol so as to contain 0.01 to 30.0 wt% of the reduced pressure concentrate and the lyophilisate.

Example 2 melanoma sites stimulating hormone (MSH) melanin synthesis of the melanocytes site inhibition experiment using

This Example is to evaluate the whitening effect to the degree of inhibition of melanin synthesis of melanocytes using melanocyte stimulating hormone (MSH) to confirm the whitening effect of the extract of the baekryeom obtained in Example 1.

The A375SM melanocytes used in this example are human-derived cell lines, which do not secrete melanin under normal conditions, but melanocyte stimulating hormone (MSH). During the artificial culture of these cells, the synthesis degree of melanin black pigment was compared and evaluated by treatment with α-MSH and baekryeum extract. The A375SM melanocytes used in this example were used from KCLB (Korea Cell Line Bank, Accession No.:80004).

Melanosite stimulating hormone is a cytokine that binds to the membrane receptor of melanocytes and transmits melanin synthesis signal into cells. The melanocyte stimulating hormone is divided into α- and β-types. Liver cross-reaction does not occur, and α-type has the same structure among all animal subjects and is widely used for the evaluation of whitening effect in the laboratory.

The melanin synthesis inhibitory effect of A375SM melanocytes using α-MSH was measured as follows.

A375SM melanocytes were dispensed in 6 well plates at a concentration of 2 × 10 ⁶ per well, cells were attached, and 200-nm of α-MSH (Sigma Co., Ltd.) and pneumococcal extracts were simultaneously treated at a concentration that did not cause toxicity and incubated for 48 hours. . After 48 hours of incubation, the cells were detached with trypsin-EDTA, the cell number was measured, and the cells were recovered by centrifugation. Intracellular melanin quantification was performed by slightly modifying the method of Rotan (Cancer Res., 40: 3345-3350, 1980). After washing the cell pellet with PBS once, 1 ml of homogenization buffer (50 mM sodium phosphate, pH6.8, 1% Triton X-100, 2 mM PMSF) was added and vortexed for 5 minutes to disrupt the cells. It was. Melt the extracted melanin by adding 1N NaOH (10% DMSO) to the cell lysate obtained by centrifugation (3,000 rpm, 10 minutes), and then melanin at 405 nm with a microplate reader (ELx800, USA). The absorbance of the melanin was measured to quantify the synthesized melanin synthesis rate (%) of the sample was measured.

Melanin synthesis inhibition rate (%) of the A375SM melanocytes was calculated by the following equation (1), IC ₅₀ value is the concentration of a substance that inhibits melanin production by inhibiting α-MSH binding to the cell membrane of melanocytes.

% Inhibition = [(A-B) / A] × 100

A: the amount of melanin in the well containing only α-MSH

B: Melanin amount in the wells to which the sample and α-MSH were simultaneously added

The result of testing the melanin production inhibitory effect of A375SM melanocytes using α-MSH showed that the IC ₅₀ value of P. aeruginosa extract was 0.01%, which was superior to that of conventional whitening agents such as kojic acid, arbutin, oil-soluble licorice extract, and lettuce extract. (Table 1).

These results suggest that the whitening extract of the present invention inhibits melanin synthesis by inhibiting the binding of the melanosite stimulating hormone α-MSH to melanocytes, unlike the conventional whitening agent, which merely inhibits tyrosinase. .

sample Melanin synthesis inhibitory effect (IC ₅₀ ) Kojisan 0 Arbutin 0 Baekryeum extract 0.01% Lettuce extract 0 Soluble Licorice Extract 0

EXAMPLE 3 Experimental Measurement of Tyrosinase Inhibitory Effect Using Mushroom Tyrosinase

This Example is to determine the whitening effect by looking at the degree of inhibition of the function of the enzyme called tyrosinase (tyrosinase) to confirm the whitening effect of the extract of the baekryeom obtained in Example 1.

Tyrosinase is an enzyme that accelerates the oxidation of tyrosine in vivo and helps melanin production. In this example, the method of inhibiting the function of this enzyme to inhibit the oxidation of tyrosine to form a black polymer called melanin (Pomerantz SH, J. Biochem., 24: 161-168, 1966) is applied. The sun whitening effect was determined.

The inhibitory effect of tyrosinase activity was investigated using lettuce extract, baekryeom extract, kojic acid, arbutin, oil-soluble licorice extract as a sample.

The inhibitory activity against tyrosinase of each sample was determined by placing 15 μl of the sample into a 96 well plate, adding 150 μl of 50 mM phosphate buffer (pH6.5), 25 μl of 1.5 mM L-tyrosine solution, and then using mushroom tyrosinase (1,500 units / ml, Sigma) 10 μl was added and reacted at 37 ° C. for 20 minutes, and then the absorbance was measured at 490 nm using a microplate reader (ELx800, USA) to measure the inhibition rate against tyrosinase. Inhibition rate (%) for tyrosinase was calculated by the following equation (2), IC ₅₀ value is the concentration of a substance that inhibits the tyrosinase enzyme activity by 50%.

% Inhibition = {[(D-C)-(B-A)] / (D-C)} × 100

A: Absorbance before reaction of the well containing the sample

B: Absorbance after reaction of the well containing the sample

C: Absorbance before reaction of the well without sample

D: Absorbance after reaction of well without sample

As a result of testing the inhibitory effect of tyrosinase activity, the IC ₅₀ value of P. aeruginosa extract was 0.02%, which was superior to the conventional whitening agents known as kojic acid, arbutin, and lettuce extract, which are known to be excellent in tyrosinase inhibition (Table 2).

sample Inhibitory effect of mushroom tyrosinase (IC ₅₀ ) Kojisan 0.037% Arbutin 0.4% Baekryeum extract 0.02% Lettuce extract 10% Soluble Licorice Extract 0.02%

[Example 4] Melanin production inhibitory effect measurement experiment using actinomycetes

This Example is to determine the whitening effect by looking at the degree of inhibition of melanin production against actinomycetes in order to confirm the whitening effect of the baekryeom extract obtained in Example 1.

Actinomycetes (Streptomyces bikiniensis NRRL B1049) were used by the Korea Science Institute (KCTC).

Actinomycetes were incubated at 28 ° C. for 2 weeks in papavizas VDYA agar slant medium (V-8 juice 200 ml, glucose 2 g, yeast extract 2 g, CaCO ₃ 1 g, agar 20 g, distilled water 800 ml, pH7.2) for 2 weeks. Spores were produced to produce spores with sterile water. 0.2 ml of spore suspension was applied to ISP No. 7 flat medium containing 0.2% yeast extract, and a paper disc moistened with 200 µl of sample (30 µg / ml) was placed on the surface of the medium. Incubated. After 48 hours of incubation, the size of the melanogenesis inhibitory ring produced was measured using 4-hydroxyanisol, which is known as a typical melanogenesis inhibitor, as a control group and the inhibition of melanogenesis was observed (Lee Chung-hwan, Journal of the Industrial Microbiology, 21: 139- 143, 1993).

Melanin production inhibitory ring against actinomycetes of P. aeruginosa was 30mm, showing melanin production inhibitory effect better than that of 4-hydroxyanisol, which is a control group, or conventional whitening agents, koji acid and arbutin (Table 3).

matter Actinomycetes Inhibitory Ring Diameter (mm) 4-hydroxyanisol 24 Kojisan 0 Arbutin 0 Hydroquinone 25 Baekryeum extract 30 Lettuce extract 12 Soluble Licorice Extract 16 p-methoxyphenol 28

Example 5 Measurement of melanin production inhibitory effect using B16F1 melanocytes

This Example was to determine the whitening effect by looking at the degree of melanin production inhibition on B16F1 melanocytes in order to confirm the effect of the baekryeom extract obtained in Example 1.

The B16F1 melanocytes used in this example are cell lines derived from mice and cells that secrete a black pigment called melanin. Samples were treated during the artificial culture of these cells to evaluate the degree of melanin black pigment reduction. The B16F1 melanocytes used in this example were used after being sold from ATCC (American Type Culture Collection, Accession No .: 6323).

The melanin biosynthesis inhibitory effect of B16F1 melanocytes was measured as follows.

B16F1 melanocytes were dispensed in 6-well plates at a concentration of 2 × 10 ⁶ per well, cells were attached, and the samples were incubated for 72 hours by treating the samples at a concentration that does not cause toxicity. After 72 hours of incubation, the cells were detached with trypsin-EDTA, and the cells were counted and centrifuged to recover the cells.

Intracellular melanin quantification was performed by slightly modifying the method of Lotan (Cancer Res., 40: 3345-3350, 1980). The cell pellet was washed once with PBS, and then 1 ml of homogenization buffer (50 mM sodium phosphate, pH6.8, 1% Triton X-100, 2 mM PMSF) was added to vortex for 5 minutes to disrupt the cells. Melt the extracted melanin by adding 1N NaOH (10% DMSO) to the cell filtrate obtained by centrifugation (3,000 rpm, 10 minutes), and then measured the absorbance of melanin at 405 nm with a microplate reader, and then quantified the melanin. Melanin production inhibition rate (%) was measured. Melanin inhibition rate (%) of B16F1 melanocytes was calculated by the following equation (3), IC ₅₀ value is the concentration of a substance that inhibits melanin production by 50%.

% Inhibition = [(A-B) / A] × 100

A: Melanin amount in wells without sample

B: Melanin amount in the wells to which the sample was added

As a result of testing the melanin production inhibitory effect of B16F1 melanocytes, the IC ₅₀ value of the baekryeom extract was 0.01%, which was superior to the existing whitening agents, koji acid, arbutin, oil-soluble licorice extract, and extract of lettuce extract (Table 4).

sample Melanin production inhibitory effect (IC ₅₀ ) Kojisan 0.05% Hydroquinone 0.03% Arbutin 0.5% Baekryeum extract 0.01% Lettuce extract 5% Soluble Licorice Extract 0.03%

Example 6 Inhibitory Effect of In Vitro Tyrosinase Enzyme Synthesis Using B16F1 Melanosite

In this example, the degree of inhibition of tyrosinase enzyme synthesis in B16F1 melanocytes was determined by measuring the amount of intracellular tyrosinase enzyme synthesis in order to confirm the whitening effect of the extract obtained in Example 1.

The B16F1 melanocytes used in this example are cell lines derived from mice and cells that synthesize an enzyme called tyrosinase. The degree of inhibition of tyrosinase enzyme synthesis was compared by evaluating the amount of enzyme synthesis by treating the sample during the artificial culture of the cells and by separating the tyrosinase from the cells.

The B16F1 melanocytes used in this example were used after being sold from ATCC (American Type Culture Collection, Accession No .: 6323).

The tyrosinase enzyme synthesis inhibitory effect of B16F1 melanocytes was measured as follows.

B16F1 melanocytes were dispensed in 6-well plates at a concentration of 2 × 10 ⁶ per well, cells were attached, and the samples were incubated for 72 hours by treating the samples at a concentration that does not cause toxicity. After 72 hours of incubation, the cells were detached with trypsin-EDTA, and the cells were counted and centrifuged to recover the cells. Wash the cell pellet once with PBS, add 1 ml of homogenization buffer (50 mM sodium phosphate, pH6.8, 1% Triton X-100, 2 mM PMSF), vortex for 5 minutes, disrupt the cells, and centrifuge (3,000 rpm). 10 minutes) to recover the supernatant. Put 1.5mM L-tyrosine, 0.06mM L-DOPA, and cell supernatant into 50mM sodium phosphate buffer (pH6.5), incubate for 30 minutes at 37 ° C, and measure the absorbance at 490nm with a microplate reader to synthesize tyrosinase enzyme. Inhibitory effect was measured. The inhibition rate of tyrosinase enzyme synthesis (%) of B16F1 melanocytes was calculated by the following equation (4), IC ₅₀ value is the concentration of a substance that inhibits 50% tyrosinase enzyme synthesis.

% Inhibition = [(A-B) / A] × 100

A: Synthesis amount of tyrosinase enzyme in wells without sample

B: Tyrosinase enzyme synthesis amount of wells to which sample was added

As a result of testing the inhibitory effect of tyrosinase enzyme synthesis of B16F1 melanocytes, the IC ₅₀ value of P. aeruginosa extract was 0.015%, which was much better than the existing whitening agents, koji acid, arbutin, oil-soluble licorice extract, and extract from lettuce. 5). In view of the above results, it can be seen that the whitening extract of the present invention exhibits a whitening effect by inhibiting the synthesis of tyrosinase enzyme, unlike the conventional whitening agent merely inhibiting the activity of tyrosinase enzyme.

sample Inhibitory effect of tyrosinase enzyme synthesis (IC ₅₀ ) Kojisan 0 Arbutin 0 Baekryeum extract 0.015% Lettuce extract 0 Soluble Licorice Extract 0

Example 7 Inflammation-Induced Enzyme Activity Inhibitory Effect Measurement Experiment

This example is to determine the anti-inflammatory effect of the baekryeomyeon extract obtained in Example 1 by determining the enzyme activity of the hyaluroniadiaze enzyme, an inflammation-associated enzyme.

Hyaluronidase is an enzyme that hydrolyzes hyaluronic acid and has the function of causing inflammation. In this example, the anti-inflammatory effect was determined by applying a method (Kakegawa Y., Japanese J. of Inflammation, 4: 437-438, 1984) to inhibit the activity of this enzyme to measure the anti-inflammatory effect.

The inhibitory effect of hyaluronidiase activity was investigated using comfrey, seesaw, triticale, oil-soluble licorice extract, and baekryeom extract as samples.

Inhibitory activity of hyaluronidiases of each sample was performed as follows.

100 μl of sample and 50 μl of hyaluronidiase solution (type IV-S, Sigma, 400U / mL) were added and reacted at 37 ° C. for 20 minutes, followed by enzyme activation solution (Compound 48/80 CaCl _{2 ·} 2H ₂ O, 100 μl of Sigma Co., Ltd., 0.1 mg / ml) was added and reacted again at 37 ° C. for 20 minutes. 250 μl of hyaluronic acid solution (0.4 mg / ml) was added thereto, followed by reaction at 37 ° C. for 40 minutes, and 100 μl of 0.4N NaOH was added to terminate the reaction. 100 μl of potassium borate solution was added thereto, reacted at 95 ° C. for 3 minutes, cooled, and 3 ml of p-dimethylaminobenzaldehyde solution was added thereto. Absorbance was measured at 585 nm to determine the inhibition rate for hyaluronidiases.

Inhibition rate (%) for the hyaluronia diase was calculated by the following equation (5), IC ₅₀ value is the concentration of a substance that inhibits the hyaluronia diase enzyme activity by 50%.

% Inhibition = [(A-B) / A] × 100

A: Enzyme activity of wells without sample

B: Enzyme Activity of Well Added Samples

As a result of testing the inhibitory effect of hyaluronidiaze enzyme activity, IC ₅₀ value of P. erythematosus extract was 0.05%, which is a useful anti-inflammatory licorice extract, comfrey extract, triticale extract, and seesaw extract, which are known to be excellent in inhibiting hyaluronidiase. It showed a superior effect (Table 6).

sample Inhibitory effect of hyaluronidiases (IC ₅₀ ) Comfrey Extract 0.22% Seesaw extract 0.58% Soluble Licorice Extract 0.08% Triticale extract 0.3% Baekryeum extract 0.05%

[Example 8] Evaluation of Skin Immune Boosting Activity

This Example was evaluated by quantifying the degree of secretion of superoxide anion produced by activated macrophages in the mouse abdominal cavity to evaluate the skin immune enhancing action of the extract of the baekryeomyeon obtained in Example 1. Immunopotentiation experiments were carried out as follows.

Macrophages are one of the cells responsible for human immunity and when stimulated by an external substance, secrete superoxide anions to dissolve external substances or release them to the outside of the cell to protect the living body. ICR mice (9 weeks old, males) were used for this immunopotentiation evaluation experiment. Macrophage cells were recovered from the abdominal cavity of the mouse and incubated for 48 hours at 37 ° C. and 5% CO ₂ incubator, and the pneumonia obtained in Example 1 using interferon and lipopolysaccharides (LPS) having excellent immunopotentiation as a control group. After adding the extract, the amount of the superoxide anion, the nitrogen dioxide anion, in the secretion of the macrophage cells was quantified at 540 nm in the visible light absorption spectrum. As a result, it was found that the addition of P. aeruginosa extract in macrophage cell cultures releases nitrogen dioxide anion about 2 times higher than that of interferon and 4 times higher than that of LPS. there was.

Example 9 Evaluation of Stimulation Relaxation Effect Using Cell Culture Technology

In this example, the cell death by Sodium Lauryl Sulfate (SLS), a substance that causes skin irritation using cell culture technology, was evaluated in order to evaluate the stimulating alleviation effect of the extract of P. aeruginosa obtained in Example 1 The degree of stimulation relaxation was evaluated to the extent of inhibition.

SLS is a substance that is used as a criterion for evaluating skin irritation based on cosmetics and is bound to cell membranes to inhibit cell metabolism and destroy cell membranes to cause skin irritation.

This example evaluates the effect of reducing cell death by SLS when SLS and P. aeruginosa extracts are added to human fibroblasts at the same time.

Hs68 fibroblasts used in this example were used as human dermal dermal cells from ATCC (American Type Culture Collection, Accession No.:1635).

Stimulation relaxation evaluation experiment using the cell culture technology was carried out as follows.

Human-derived fibroblasts were dispensed in 96 well plates at a concentration of 1 × 10 ⁵ per well, incubated for 24 hours, treated with 0.01% SLS alone, 0.01% SLS and pneumoniae extract (0.01%) simultaneously and added for 24 hours. Incubated. After incubation, MTT (Sigma) reagent was added and incubated for 4 hours, the culture medium was removed, 1N NaOH / isopropanol solution was added and stirred for 20 minutes, and the absorbance was measured at 565 nm using a microplate reader. The effect of inhibiting apoptosis by was measured.

The test results showed that the extract of P. leucine is an irritant that reduces skin irritation that can occur due to cosmetic substrates by inhibiting cell death induced by SLS (Table 7).

sample Fibroblast survival rate (%) 0.01% SLS 25 0.01% SLS + 0.01% White Pleat Extract 80

[Examples 10 to 12 and Comparative Example 1]

This Example prepared the cosmetic containing the extract of baekryeom obtained in Example 1 and evaluated the skin whitening effect in a comparative experiment with Comparative Example 1 in humans.

The cosmetics used in the comparative experiments are in the form of a cream, the composition of which is shown in Table 8. First, the phase b) recorded in Table 8 is heated and stored at 70 ° C. To this, a) phase is added, and after pre-emulsification, it is emulsified uniformly with a homomixer, and then cooled slowly to prepare a cream (Examples 10 to 12, Comparative Example 1). For 20 experimenters (20-35 year old female), the cream prepared in Examples 10 to 12 was applied to the right side of the face and the cream prepared in Comparative Example 1 was applied to the left side of the face twice a day for 2 consecutive months. It was. After completion of the experiment, the skins of the application areas on the left and right sides of the face were compared by using an image analyzer and a color difference meter, and the darkest color was set to 5, the middle color to 3, and the brightest color to 1. Table 9 compares the facial skin color of the experimenter using the cream prepared in Example 12 with the facial skin color of the experimenter using the cream made in Comparative Example 1.

As shown in Table 9, it can be seen that the whitening effect is excellent in the facial skin of the experimenter who applied the cream containing baekryeom extract.

Raw material Example Comparative Example 1 10 11 12 end Stearyl alcohol 8 8 8 8 Stearic acid 2 2 2 2 Stearic Acid Cholesterol 2 2 2 2 Squalane 4 4 4 4 2-octyldodecyl alcohol 6 6 6 6 Polyoxyethylene (25 mol addition) alcohol ester 3 3 3 3 Glyceryl monostearic acid ester 2 2 2 2 I Baekryeum extract 10 One 0.1 - Propylene glycol 5 5 5 5 Purified water Quantity Quantity Quantity Quantity

Note) Unit: Weight%

Used products Example 12 Comparative Example 1 Experiment number Right skin color Left skin color One 1.5 2.5 2 2 3 3 2 3 4 1.5 3 5 2.5 3.5 6 3 4 7 1.5 2.5 8 2 3 9 2 3 10 2.5 3.5 11 1.5 3 12 2.5 3 13 2 3 14 3 4 15 2.5 3 16 2 3 17 2 3.5 18 2.5 3 19 2 3 20 2 3

Example 13

This Example is to evaluate the stimulating alleviation effect of the cosmetics containing the baekryeom extract obtained in Example 1 by human patch experiment.

This evaluation is a result of evaluation obtained by directly applying the result obtained in Example 9 to the human body. SLS alleviates the effects of irritation on the basis of irritation index by mixing SLS that causes irritation to general cosmetic prescriptions (cream, lotion, skin, essence) and the products prepared in Examples 10 to 12 for 24 hours, 48 hours and 72 hours Is evaluated.

FINN CHAMBER (FINLAND) was used to coat 0.2 mg of each product on the upper arm of 50 healthy men and women aged 20-50 years, and the acute stimulation index was evaluated after 24 hours. After evaluation, the same amount of product was applied to the same site again, and the delayed stimulation index after 48 and 72 hours was evaluated.

The test resulted in no skin redness even after 24 hours, 48 hours, 72 hours of patching the product containing the baekryeom extract.

The results of this evaluation indicate that there is a significant effect of reducing skin irritation by substrates (surfactants, fragrances, alcohols) that cause irritation when the extract of Pleurotus erythematosus is used in cosmetics.

Other examples are shown below. That is, as shown above, the skin whitening effect, the stimulating alleviation effect, the immune enhancing effect and the like showed the excellent effect on skin improvement.

[Example 14] Preparation of the lotion containing the baekryeom extract obtained in Example 1

To 8 g of 95% ethanol, 0.05 g of polypyrrolidone, 0.1 g of oleyl alcohol, 0.2 g of polyoxyethylene monooleate, 0.2 g of fragrance, 0.1 g of paraoxybenzoic acid methyl ester, a small amount of antioxidant and a small amount of pigment are dissolved and mixed. It was. 0.05 g of baekryeom extract obtained in Example 1, 5 g of glycerine was dissolved in 85.33 g of purified water was added to the mixed solution and stirred to obtain a lotion having a skin improvement effect.

Example 15 Preparation of Latex Containing the Pleurotus Extract Obtained in Example 1

1.2 g of cetyl alcohol, 10 g of squalane, 2 g of petrolatum, 0.2 g of paraoxybenzoic acid ethyl ester, 1 g of glycerin monoesteralide, 1 g of polyoxyethylene (20 mole added) monooleate and 0.1 g of fragrance were mixed and dissolved at 70 ° C. , 0.5 g of the white chlorosis extract obtained in Example 1, 5 g of dipropylene glycol, 2 g of polyethylene glycol 1500, 0.2 g of triethanolamine, and 76.2 g of purified water were dissolved by heating to 75 ° C. Both mixtures were emulsified and cooled to obtain an emulsion having an oil-in-water (O / W) type skin improvement effect.

Example 16 Preparation of Cosmetic Liquid Containing the Pleurotus Extract Obtained in Example 1

5 g of 95% ethyl alcohol, 1.2 g of polyoxyethylene sorbitan monooleate, 0.3 g of kitulose, 0.2 g of sodium hyaluronate, 0.2 g of vitamin E-acetate, 0.2 g of sodium licorice, 0.1 g of paraoxybenzoic acid ethyl ester, 1 g of the baekryeom extract obtained in Example 1 and a suitable amount of pigment was mixed to obtain a cosmetic liquid having an effect of improving the skin.

As described above, the baekryeom extract according to the present invention, the inhibition of cell membrane binding of melanocyte stimulating hormone (MSH), tyrosinase activity inhibitory effect, which prevents the production of melanin, a substance causing blemishes, freckles and skin pigmentation, Skin whitening effect such as tyrosinase synthesis inhibitory effect, melanin synthesis inhibitory effect, skin cell death suppression effect that reduces skin irritation caused by cosmetic substrates, and skin stimulation alleviation effect such as inhibitory activity of inflammation-related enzymes The effect was shown. Therefore, it can be seen that the cosmetic composition, such as a lotion, cream, milky lotion, pack, etc. containing the baekryeom extract has a skin lightening effect, irritation-releasing effect and immune enhancing effect.

Claims (7)

Cosmetic composition for skin whitening containing baekryeom extract as the main active ingredient. The extract of claim 1, wherein the extract is extracted using at least one solvent selected from purified water, lower alcohol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane, and hexane. Cosmetic composition for skin whitening. The cosmetic composition for skin whitening according to claim 2, wherein the composition is extracted using a 40-95% ethanol solution as an extraction solvent. The cosmetic composition for skin whitening according to claim 2 or 3, wherein the extraction is performed by a cooling or heating method. The cosmetic composition for skin whitening according to claim 4, which is obtained by performing filtration, concentration or lyophilization after extraction. The cosmetic composition for skin whitening according to any one of claims 1 to 3, wherein the composition contains an extract of 0.01 to 30% by weight. The cosmetic composition according to any one of claims 1 to 3, wherein the cosmetic composition is selected from a lotion, gel, water-soluble liquid, oil-in-water (O / W) type and water-in-oil (W / O) type formulations. Cosmetic composition for skin whitening containing baekryeom extract.