KR20020070674A - Novel fermented feed for fowl and production prosess thereof - Google Patents

Abstract

PURPOSE: Provided is a process of producing eggs using new fermented feed obtained by mixing red ginseng by-products with rice bran and a microorganism composite preparation, fermenting and then mixing quartz porphyry powder. Whereby, the eggs are high in freshness, palatability and stability. CONSTITUTION: A red ginseng by-product and rice bran are subjected to preliminary fermentation at 60deg.C for 24hr and then sterilization. The preliminary ferments are mixed with 0.1 to 0.2% by weight of a microorganism composite preparation of S. cerevisiae KCTC1028 yeast strains, L. acidophilus KCTC3199 lactic acid bacteria producing strains, B. subtilis KCTC1028 strains, A. oryzae KCTC6983 fungus strains and then fermented at 40deg.C for 12hr. Thereafter, the ferment is dried with hot air and added with quartz porphyry powder in a weight ratio of 50:50 and 15 to 30g fermented feed is fed four times per day to chickens of about 140 days after hatch and raised without the injection of antibiotics.

Description

Novel fermented feed for fowl and production prosess

The present invention relates to a fermented feed using red ginseng byproducts and a method for producing eggs using the same. More specifically, the present invention is mixed with microbial complex bacteria such as yeast, lactic acid bacteria, Bacillus bacteria, yeast fungus and rice bran to fermentation in a microbial growth fermenter after mixing red ginseng by a mixed salary and a certain ratio in the blended feed freshness, The present invention relates to a method for mass-producing eggs with high palatability and safety.

For the production of conventional eggs has been feeding the laying hens as a rich feed produced by the manufacturer of livestock feed, and due to the overfeed of the rich feed has caused chickens various metabolic diseases. For example, over-administration of antibiotics, including fatty liver disease and hyperacidity in laying hens, resulted in worsening of the health of laying hens and the production of low-quality eggs containing large amounts of antibiotics. In addition, because of the dependence on imported grains, huge foreign currency was wasted and the exchange rate fluctuated severely, causing feed prices to soar, resulting in supply and demand imbalance due to logistics delivery, which has caused serious problems on both sides.

The present invention has been made in view of the above problems, and the yeast, lactic acid bacteria, Bacillus genus bacteria, yeast, which can be easily obtained from microorganisms deposited by red ginseng by-products and rice bran in agricultural resources that are discarded among farm by-products. It is an object of the present invention to provide a fermented feed composition for laying hens by adding mixed microorganisms such as mold and fermentation in a microbial growth incubator and then adding and mixing a certain ratio of ganban stone powder according to the growing stage of the laying hens.

Another object of the present invention is to provide a method for producing eggs having high freshness, palatability, and safety by administering the fermented feed composition for laying hens. Another object of the present invention to provide an egg produced by the above method.

The above object of the present invention is a fermentation process by mixing the microorganism complex preparation in the rice bran, which is a by-product of rice ginseng easy to obtain from the farm, red ginseng by-products discarded in the red ginseng manufacturing process, and then mixed with the ganban stone powder, it is administered to the egg Achieved by evaluating the quality of

Hereinafter, the specific configuration of the present invention will be described, but the scope of the present invention is not limited to these, but includes both simple numerical values and process changes.

1 is a schematic process diagram showing a method for producing a fermented feed of the present invention

The present invention is a fermentation feed for laying hens by adding ganban stone powder to the fermented product by mixing the pre-fermented bacterium of red ginseng by-product and the pre-fermented steril of rice bran as described in FIG. Prepared.

The microbial complex used in the present invention is a suitable mixing agent of yeast strain, lactic acid strain, Bacillus strain, yeast mold strain.

The red ginseng by-product is classified as a waste generated from the manufacturing process of red ginseng produced by increasing the fresh ginseng for a long time.

By-products of red ginseng contain a large amount of antioxidants such as maltol, which is found in fresh ginseng, and saponins, as well as those necessary for the biosynthesis of humans and chickens, and carbon and nitrogen sources during microbial fermentation. Is used.

On the other hand, rice bran is used as a useful carbon source of microorganisms in the present invention as a farm by-product remaining after the rice is milled.

Yeast strains, lactic acid strains, Bacillus strains, yeast fungi strains used as a microbial complex in the present invention were used to receive the deposit in the domestic microorganisms deposit institution. In other words, these yeasts and bacteria such as lactic acid bacteria and Bacillus strains and yeast fungus were used as a complex preparation because they can be cultured even if they are fermented in complex, S. cererisiae KCTC1028, L obtained from the Korea Institute of Biotechnology Research acidophilus KCTC3199, B. subtilis KTCT1028 and Aspergilus oryzae KCTC6983 were used. The microbial mixture is a mixture of 4.0 × 10 ⁷ cfu / g, 3 × 10 ⁷ cfu / g, 3 × 10 ⁷ cfu / g and 2 × 10 ⁷ cfu / g, respectively, and the weight of the raw material mixture when added to the fermentor 0.1 to 0.2% of the weight is used.

In addition, the elvan rock powder added as a feed for laying hens in the present invention may be produced anywhere in Korea.

The fermentation product 50: ganban stone powder 50 is preferably mixed when the microorganism composite material is added to the fermentation medium and the ganban stone powder is added to the fermentation product obtained by fermentation in a fermentation tank. Elvan powder is a source of minerals required for laying hens, and is added to laying hens' feed to significantly reduce ammonia and sulfur gas effluent from chicken distribution and significantly improve the physiological activity of laying hens.

Below. Specific inventions of the present invention will be described with reference to Examples.

Example 1 The Invention Feed Product and Its Preparation

100 kg of red ginseng by-product having 90% water content was weighed and put into a fermentation treatment machine (ACE animal product), and after adjusting to a temperature of 60 ° C., it was pre-fermented and sterilized for 24 hours.

170 kg of rice bran was added to the pre-fermentation product of red ginseng by-product to maintain 40% water content, and then mixed and sterilized for 24 hours at the same temperature (60 ° C.).

The red ginseng by-product and the pre-fermentation product of rice bran mixture were mixed, and then, 1 kg of the microbial composite material was added thereto, mixed, and fermented for 12 hours while maintaining the temperature of the fermentor at 40 ° C. The fermented feed composition is dried with a hot air dryer to achieve a water content of 10%, and 250 kg of ganban stone powder is mixed to produce 500 kg of the final product and stored at 4 ° C in a large refrigerator to be used as a feed material for laying hens. It was.

Experimental Example 1: Analysis result of ginseng active ingredient of the feed of the present invention

Results of ginseng active ingredient analysis of the feed of the present invention The results of the ginseng active ingredient analysis of the fermented feed composition of the present invention produced according to the above examples are shown in Table 1 below.

[Table 1] Analysis results of ginseng active ingredient

Korea Ginseng and Tobacco Research Institute (2000.6.9)

Test Items result Test standard Investigation-ponin (mg / 100g) 220 Reference test Ginsenoid Rg1 (mg / 100g) 2.37 Reference test Ginsenoid Re (mg / 100g) 7.56 Reference test Ginsenoid Rd (mg / 100g) 3.93 Reference test Ginsenoid Rc (mg / 100g) 4.71 Reference test Ginsenoid Rb2 (mg / 100g) 5.34 Reference test Ginsenoid Rb1 (mg / 100g) 10.19 Reference test Note : 1. Client and address: Gil-Han Han. Changwan Farm 511-1 Buyeon-ri, Buk-myeon, Cheongwon-gun, Chungbuk 2. Purpose of test: Self-quality inspection 3. Date of Receipt: May 31, 2000

Experimental Example 2 Microbial Analysis of the Invention Feed

In order to evaluate the quality of the feed product of the present invention, useful microorganisms and other harmful microorganisms were analyzed. The density measurement of microorganisms was carried out by dilution agar plate method, total bacteria and Bacillus were nutrient agar, yeast and fungus 50% of ampicillin potato dextrose Agar, lactic acid bacteria were MRS-BCP agar, E. coli was 3M petrifilm, Salmonella like bacteria were selenite Tested on agar. The dilution was preincubated for 1 hour in a 30 ℃ shaking incubator for 1 hour in 9 ml of saline water (saline water) and then diluted again by 1/10 was incubated in a 30 ℃ incubator. Lactobacillus and E. coli were cultured in a 37 ℃ incubator.

The microbial density was measured twice in 24 hours and 36 hours after incubation in a thermostat, and the microbial density after 36 hours was used.

All experiments were performed in three repetitions and the results were averaged over three repetitions. The moisture content of the sample was measured in a dryer at 105 ° C. until the content was dried, and then measured.

The test results are shown in Table 2.

Table 2 Test Results

Test Items Microbial Density (Log cfu / g) Sample A Total bacteria 6.9 Bacillus 5.3 mold 3 or less Yeast 3 or less Lactobacillus 3 or less Escherichia coli 3 or less Salmonella Lactobacillus 3.8 Note; The microbial density (Log cfu / g) is the number of microorganisms contained in 1 g (gram) of the sample 2. Moisture content (%) is 9.843. PH is 5.16

Example 2 Feeding Experiments on Laying Hens of the Invention

The present inventors feed four times a day using the feed prepared by the method of Example 1 with respect to 5000 number of laying hens 140 days old after hatching, and the feeding amount per feed is 15-30 g. Over 80 weeks of mechanical age, the birds were raised and harvested. Of course, no antibiotics were administered at this time. Quality evaluation of the eggs of the present invention was measured after 7 days refrigerated eggs of 41 weeks old egg production.

As a result of evaluating the quality of the feed grade eggs of the present invention, freshness, palatability, and safety were all significantly higher than those of general eggs and commercial eggs, as shown in Table 3.

Experimental Example 1 Freshness Measurement of Eggs of the Invention

48 eggs of the present invention produced by the method of Example 2 and the control (produced by feeding the general feed) eggs were broken on a flat plate and then the height of egg yolk and egg white was measured. The average freshness of eggs produced at 41 weeks of age was 96 HU, which was higher than that of 70 HU eggs.

Table 3 Egg Quality Test Results Table of the Invention

(41-week-old production egg: measured 7 days after refrigeration)

Item Farm Name Measure () Eggshell Color (%) Egg weight (g) Egg yolk height (mm) Freshness (HU) Eggshell weight (g) Eggshell density (mg / ㎠) Eggshell thickness (mm) Eggshell strength Blood ovulation (%) Foreign substance column (%) Egg yolk (Roche) Changwan Farm 48 32 63.5 7.6 86 6 80.4 0.358 4.2 6.3 12.5 9.8 General farm 48 33 64.4 4.9 66 6.4 85.4 0.351 2.9 12 30 9.4 Municipal market 50 34 64.4 4.8 63 6.6 84 0.388 3.2 16 30 11.1 5 months refrigerated storage 10 29 62.6 3.9 52 6.7 - 0376 4.0 15 29 11

(Difficult quality measuring machine: QCM Egg Quality Tester.Made in England)

Experimental Example 2: Evaluation of palatability and stability by measuring blood ovulation and foreign substances of the present invention

When the egg of the present invention produced by the method of Example 2 and the control egg (the same as Experimental Example 1) were broken, the absence of the appearance of hemoglobin and foreign body eggs appearing in egg yolk and egg white would indicate palatability and safety. .

The egg ovulation appearance rate of the eggs of the present invention was 6.25%, the appearance of foreign matter was 12.5% and was measured at an angle of more than 50% compared to the control, and evaluated as having high palatability and safety to consumers. (See Table 3)

Experimental Example 3: Evaluation of eggshell color, egg weight, egg yolk color of egg yolk, eggshell weight, eggshell frequency eggshell thickness, eggshell strength of egg of the present invention (see Table 3)

It was measured by the egg quality measuring machine QCM egg quality tester (made in England) of the egg of the present invention 48 and the control (same as Experimental Example 1) produced according to the method of Example 2.

Eggs of the present invention in eggshell color were 33% and 34% in the control and commercial eggs, respectively, whereas eggs of the present invention were excellent.

Egg weight (g) was 63.5 for eggs of the present invention, the average was 64.4, 64.4 for control and commercial eggs, respectively, and the height of egg yolk was 7.6 mm, whereas 4.9 mm and 4.8 mm for eggs and control eggs, respectively. Appeared. The higher the yolk height, the higher the freshness, and the higher the freshness of the egg of the present invention was measured by about 40% or more.

There was no difference in egg yolk (roche), and egg weight (g) of the present invention was 6, while the average egg weight of the control and commercial eggs was 6.4 and 6.4, respectively. Eggshell density (mg / ㎠) and eggshell thickness (mm) were not significantly different compared to the control or commercially available eggs as a standard for maintaining product value at. In particular, in the present invention, the value was measured as 4.2 for eggshell strength. In contrast, the control eggs or commercial eggs were measured to be 2.9 and 3.2, respectively, indicating that the eggs of the present invention are remarkably superior.

These experiments were able to produce excellent eggs because the microbial complex and its fermentation products (red ginseng by-products and rice bran decomposed products) and ganbanite components containing a large amount of minerals were fed to laying hens and transferred to eggs.

The present invention obtains microbial fermented feeds based on red ginseng by-products and rice bran, which are discarded among farm by-products by the microbial complex strains that do not exist so far as is apparent from the above-described examples and experimental examples, and the traces essential for growing laying hens There is an effect of providing a fermented feed mixed with the elvan rock powder containing a large amount of ingredients. In addition, the present invention has a high freshness, palatability and safety by feeding the fermented feed to the laying hens, and has an excellent effect of producing high-value eggs that are not broken in the process of handling, storing and transporting eggs. It is a very useful invention.

Claims (3)

Preliminary fermentation and sterilization of red ginseng by-product at fermenter temperature of 60 ° C. for 24 hours; Mixing the preliminary fermentation products; Fermentation treatment of S. cerevisiae KCTC1028 yeast strain, L. acidophilas KCTC3199 lactic acid strain, B. subtilis KCTC1028 strain, and A. oryzae KCTC6983 fungal strain microbial mixed agent for 12 hours at 40 ° C .; Hot-air drying the fermented product to a moisture content of 10% or less; Fermented feed for laying hens, characterized in that prepared by adding and mixing the ganban stone powder in a weight ratio of 50:50 relative to the fermented product. Total bacteria 6.9cfu / g, Bacillus 5.3cfu / g, mold 3cfu / g, yeast 3cfu / g, lactic acid bacteria 3cfu / g, Escherichia coli 3cfu / g, Salmonella Lactobacillus 3.8cfu / g obtained by the method of claim 1, Fermented feed for laying hens, characterized in that the content is less than 10% and less than pH 6.0. After fermentation of 140 days after hatching, feed the fermented feed according to item 2 above to 15-30g once a day and feed 4 times a day and breed without antibiotics. Egg freshness is more than 80HU, egg ovulation appearance rate of 6.3%, foreign body appearance rate of 12.5% level, egg yolk height of 7.6㎜ level, egg strength is characterized by 4.2 levels.