KR101137893B1 - Composition for feed additive comprising fermented red ginseng marc and Method for preparing the same - Google Patents

Abstract

The present invention relates to a feed additive composition comprising the fermented red ginseng bak as an active ingredient and a method for producing the same.

Description

 Composition for feed additive comprising fermented red ginseng marc and Method for preparing the same}

The present invention relates to a feed additive composition comprising a fermented red ginseng bak as an active ingredient and a method for producing the same, and more particularly, to inoculate a strain useful as a livestock probiotic in red ginseng bak to produce a fermented red ginseng bak, and to prepare a feed additive comprising the same. It relates to the feeding efficacy of laying hens, broilers, weaning pigs.

In general, in order to prevent disease of the livestock and increase the productivity of the livestock, many antibiotics have been used as growth promoters, and the environment has become increasingly polluted due to the abuse of these antibiotics. Therefore, in recent years, the livestock industry has restricted the use of antibiotics in order to achieve eco-friendly breeding, and the demand for non-antibiotic livestock products is increasing.

Various natural substances have been studied as substitutes for antibiotics as growth promoters, and the results of researches that double the efficacy through a process called fermentation have recently been reported. Plants with excellent physiological activity resulted in various reactions caused by microorganisms through fermentation, and as a result, physiological activity increased from 1 to as many as 13 times (Process Biochemistry 40: 2757-2762; Lin et al., 2005; Plant Food for Human Nutrition 61: 157-160; Sato et al., 2006; Applied Microbiology and Biotechnology 75: 763-768; Wang et al., 2007).

Korean red ginseng has long been known to have a high preventive effect against various diseases. Accordingly, various industrial uses are being made not only for oriental medicine and folk medicine but also for food. The effect of red ginseng is known to originate mainly from polysaccharides, and contains about 20 to 30%, and the variety is about 21 species. The physiological activity of red ginseng is expected to improve the condition of livestock and to prevent disease. The efficacy of red ginseng on stress, cardiovascular, immune and endocrine function has been scientifically proven in various studies (Journal of Enthnopharmacology 111: 567-572; Jiang et al., 2007; Neucroscience Letter 343: 62 -66; Kim et al., 2003; Pharmacology Research 54: 46-49; Lee et al., 2006; Indian Journal of Medical Research 124: 199-206; Shin et al., 2006).

However, the use of red ginseng as a feed additive in itself is hardly feasible in terms of economics, but the red ginseng gourd produced in the process of producing red ginseng extract can be used as a feed additive due to its high economic efficiency. Red ginseng gourd is rich in dietary fiber and contains some ingredients and flavors of red ginseng, making it an excellent material. In addition, it is reported that red ginseng leaves a large amount of acidic polysaccharides with excellent physiological activity (Korean Ginseng Journal 26: 202-205; Lee Jong-won, Do Jae-ho, 2002).

In addition to the acidic polysaccharides, red ginseng gourd contains various carbohydrates, which helps the growth of microorganisms. As a result of culturing yeast in a medium containing red ginseng gourd, it has been reported that the addition of red ginseng gourd increases the cultivation efficiency by 30-40% and increases the amino acid content in yeast cells (Korea Journal of Forestry & Forestry 10: 1-10; Kim Sang-Dal et al., 1986).

Therefore, the red ginseng extract obtained as a by-product from the red ginseng extract process can be used as a livestock feed additive due to the presence of various physiologically active substances, and can improve the productivity of the livestock by enhancing its efficacy through the fermentation process. As a result, by-products are recycled through the fermentation process and their added value can also be significantly increased.

The present invention has been made by the necessity of the above, an object of the present invention is to provide a method of using the red ginseng gourd as a feed additive obtained by the red ginseng extraction process.

In order to achieve the above object, the present invention provides a livestock feed additive composition comprising the fermented red ginseng meal as an active ingredient.

In a preferred embodiment of the present invention, the fermented red ginseng gourd is preferably obtained by inoculating Bacillus subtilis in red ginseng after primary fermentation, but not always limited thereto.

In another embodiment of the present invention, the red ginseng foil is preferably included in the range of 20 to 30% by weight in the total composition, but is not limited thereto.

In another embodiment of the present invention, the second fermentation of the composition is at least one selected from the group consisting of Lactobacillus asidophilus, apsifergus orize, Saccharomyces cerevises, and Streptomyces thermophilus. It is preferable to further include a probiotic, but is not limited thereto.

In a preferred embodiment of the present invention, the composition is 10 to 30% red ginseng meal, 5 to 30% soybean meal, 5 to 30% wheat flour, 1 to 15% starch, 1 to 15% molasses, and 1 to 1 corn powder. It is preferable to contain 15%, but is not limited thereto.

In addition, the present invention (a) inoculated Bacillus subtilis (Bacillus subtilis) in the red ginseng gourd fermentation for 24 to 72 hours at 30 ~ 40 ℃; and (b) obtained through the step (a) A second stage fermentation process by adding at least one probiotic selected from the group consisting of Lactobacillus asidophilus, acipiferus duckase, Saccharomyces cerevisiae, and Streptomyces thermophilus, to a composition comprising fermented red ginseng meal. It provides a method of producing a livestock feed additive composition through.

The feed obtained by adding the fermented red ginseng gourd of the present invention is used as a feed for animals such as mammals, birds, reptiles, amphibians and fish. For example, the feed of the present invention is used as a pet food such as dogs, cats and mice, animal feed such as cattle and pigs, poultry feed such as chickens and turkeys, and fish feed such as sea bream and defense. do.

The feed of the present invention can be made by appropriately blending the fermented red ginseng meal composition of the present invention to a feed material. Examples of feed materials include cereal grains, crude steel, vegetable oils, animal feedstuffs, other feedstuffs, and refined products.

Examples of cereals include maize, wheat, barley, oats, rye, brown rice, buckwheat, buckwheat, sorghum, blood, corn, soybeans and the like.

Examples of the crude steel include rice bran, defatted rice bran, wheat bran, horse meal, wheat, embryo, barley bran, screening, pellets, corn bran, and corn embryo.

Examples of vegetable oils include soybean meal, soybean meal, flaxseed meal, cottonseed meal, groundnut meal meal, safflower meal, palm oil palm, palm oil, fomarak, sunflower oil, rapeseed meal, cowpea oil and mustard oil.

As animal feed materials, for example, fish meal (North American wheat, imported wheat, whole wheat, coastal wheat), fish bulrush, meat meal, fish bone meal, blood meal, decomposing hair, bone meal, animal by-products, feather mill, silkworm, skim milk powder, Casein, dried whey, and the like.

Other feedstocks include plant foliage (alfalfa, haycube, alfalfa leaf powder, pseudo acacia powder, etc.), corn processing industrial by-products (corn gluten, wheat, corn gluten feed, corn staple, etc.), starch processed products, sugar, fermentation. Industrial products (yeast, beer foil, malt sprouts, alcohol foil, walnuts, etc.), agricultural production by-products (citrus fruits, tofu, coffee, cocoa, etc.), others (cassava, bean, guam, seaweed, Krill, spirulina, chlorella, minerals, etc.) etc. are mentioned.

Typical products include proteins (casein, albumin, etc.), amino acids, sugars (starch, cellulose, sucrose, glucose, etc.), minerals, vitamins, and the like.

By routinely ingesting the animal feed of the present invention in an animal, the desired effect of the present invention can be achieved.

As can be seen through the present invention, the livestock feed additive composition comprising the fermented red ginseng meal of the present invention is effective in increasing the antioxidant activity, feed efficiency and poultry production as a result of feeding to laying hens, broilers, weaning pigs and It has improved egg productivity, high egg weight, egg thickness, etc., and also provides excellent effects such as an increase in the daily weight of weaning piglets.

1 is a graph of the determined concentration of red ginseng gourd for the preparation of fermented red ginseng gourd using Bacillus subtilis.
Figure 2 is a diagram illustrating a manufacturing process of a livestock feed additive based on fermented red ginseng gourd.

Hereinafter, the present invention will be described in more detail with reference to non-limiting examples. However, the following examples are described for the purpose of illustrating the present invention and the scope of the present invention is not to be construed as being limited by the following examples.

Example 1: Red ginseng gourd fermentation using Bacillus subtilis

Red ginseng extract was extracted and the contents of red ginseng gourd were included in the culture composition for fermentation of the remaining red ginseng gourd. The basic culture composition was dry weight, soybean meal 30%, wheat bran 20%, starch 5%, molasses 5%, and corn powder 10%, and red ginseng meal added at 0%, 1%, 5%, 10%, 20% was used, and the rest was filled with Perlite, and the total water content was 70%. The red ginseng culture composition was sterilized for 30 minutes at a temperature of 100 ℃ or more. The bactericidal culture composition was inoculated with Bacillus subtilis 1-5% incubated at 1.0x10 ⁹ cfu / ml or more.

As a result of measuring the number of bacteria grown after incubating the inoculated red ginseng cultivation culture at 30 ° C. for 48 hours, as shown in FIG. Cell growth was shown. The red ginseng gourd content for fermented red ginseng meal was determined to be 30%.

Example 2 Comparison of Antioxidant Activity Before and After Fermentation

The antioxidant activity was evaluated after fermenting red ginseng meal according to the conditions of Example 1. Antioxidant activity was evaluated using free radical scavenging activity using DPPH (α, α'-diphenyl-β-picrylhydrazyl).

As a result, as shown in Table 1 below, the antioxidant activity was improved by five times or more compared to before fermentation.

Table 1 is a table showing the difference in antioxidant activity before and after fermentation of red ginseng.

Example 3: manufacturing process of fermented red ginseng

Fermented red ginseng gourd was prepared on the basis of the fermented red ginseng gourd production process obtained through the above example, and after drying it, Lactobacillus acidophilus, Aspergillus orize, Aspergillus oryzae, Saccharomyces ceres Saccharomyces cerevisae, Streptomyces or Streptococcus thermophilus or a combination of probiotics were mixed to prepare a composition for livestock feed through a second fermentation process for 24 hours. The manufacturing process of the livestock feed additive based on the fermented red ginseng gourd is as shown in FIG.

Seed culture in Figure 2 means the culture of Bacillus subtilis (Bacillus subtilis) for red ginseng gourd fermentation.

In Figure 2, the red ginseng gourd fermentation process means the first fermentation of Bacillus subtilis in the culture composition containing the red ginseng gourd of Example 1.

In FIG. 2, the second fermentation process is performed to improve the efficacy of fermented red ginseng gourd (Lactobacillus acidophilus), Aspergillus orize (Aspergillus oryzae), Saccharomyces cerevisae (Saccharomyces cerevisae), Streptococcus Probiotics such as Mises thermophilus (Streptococcus thermophilus) are mixed and incubated for 24 hours.

In Figure 2, the moisture control process is a process of controlling the moisture by mixing the excipients to facilitate the grinding of the fermented red ginseng gourd secondary fermentation is completed.

Example 4: Preparation and Specification Evaluation of Livestock Feed Additive Based on Fermented Red Ginseng

According to Example 3, a livestock feed additive containing a fermented red ginseng gourd was prepared and subjected to specification evaluation for laying hens, broilers, and weaners.

1) Broiler Specification Evaluation

Fermented red ginseng gourd feed additives were added to broiler feed for 4 weeks to evaluate the results. As shown in Table 2, the results were obtained. The experimental group fed fermented red ginseng meal showed significantly higher end weight than the control group that did not receive fermented red ginseng meal, but no significant difference was found in the amount of feed consumed during the experimental period. As a result, feed efficiency and poultry production were increased by fermented red ginseng meal.

Table 2 shows the effect of fermented red ginseng gourd on broiler chicken growth and chicken breast production.

2) Evaluation of laying hens

The effects of fermented red ginseng meal on the productivity of laying hens were investigated. The effects of fermented red ginseng gourd were evaluated after laying for 12 weeks after adding 0.1% fermented red ginseng gourd to the feed. The results are shown in Table 3. The experimental group fed fermented red ginseng meal showed significantly better egg productivity and significantly higher egg weight than the control group not fed fermented red ginseng meal. Egg mass and egg thickness were also improved by fermented red ginseng meal.

 Table 3 shows the effect of fermented red ginseng gourd on the productivity of laying hens.

3) Effect of Fermented Red Ginseng Leaves on Growth of Weaning Pigs

In order to investigate the effect of fermented red ginseng meal on the growth of weaning piglets, 4% -week-old fermented red ginseng meal was added with 0.1% fermented red ginseng meal. The results are shown in Table 4. Feeding fermented red ginseng gourd increased the daily weight gain of weaning piglets and improved feed efficiency. Feeding fermented red ginseng meal resulted in the reduction of immune substances in the blood of weaning piglets, indicating that the feeding status of weaning piglets was very stable.

Claims (6)

delete delete delete delete delete (a) Red ginseng meal is added to the basal culture composition consisting of soybean meal, wheat flour, starch, molasses and corn powder, inoculated with Bacillus subtilis and fermented at 30-40 ° C. for 24 to 72 hours. Making a; and
(b) selected from the group consisting of lactobacillus asidophilus, acipiferus duck, saccharomyces cerevis, and streptomyces thermophilus in a composition comprising fermented red ginseng gourd obtained through step (a). A method of preparing an animal feed additive composition with improved antioxidant activity, including the second fermentation step by adding at least one probiotic.

Images