KR101705320B1 - Method of manufacturing fermented feed by medium of much viable cell and the fermented feed thereof - Google Patents

Abstract

The present invention discloses a method for producing a fermented feed.
A method for producing a fermented feed according to the present invention comprises the steps of: preparing a first functional water containing sodium and potassium ions; S2 step of preparing a second functional water containing zinc and selenium ion; A step S4 of mixing and extracting the first functional water and the second functional water, a step S4 of mixing and extracting the beer dry yeast with the first functional water and the second functional water, and a step S4 of adding sugar water to the soybean meal extract and beer dry yeast extract, After step S5 of preparing a culture solution inoculated with Saccharomyces cerevisiae boulardii, the protein solution and the molasses are mixed with water, and then the first functional water and the second functional water are added. The culture solution is mixed and fermented, (Step S6). In this case, various symptoms such as constipation, dilatation, northern bloating, intestinal fermentation and the like are improved to prevent a formal effect (absorption of harmful bacteria), prevention of diarrhea, improvement of digestibility, Jin, can enhance the feed efficiency, improved quality of fattening, hangbyeongryeok and immunity.

Description

FIELD OF THE INVENTION The present invention relates to a fermented feed, and more particularly,

The present invention relates to a method for producing fermented feed and a fermented feed thereof. More particularly, the present invention relates to a method for producing fermented feed, and more particularly, to a fermented feed, which is improved in various symptoms such as constipation, dilatation, northern bloating and intestinal fermentation to prevent diarrhea, A method for manufacturing a fermented feed that improves the quality of meat, enhances the anti-illness and immunity, and a fermented feed thereof.

In general, the feed used for raising livestock, etc., is mainly used as a natural formulated feed mixed with various cereals.

However, the protein, fat, and carbohydrate of the grains contained in these feeds formed a strong binding force so that the digestion and absorption rate of the livestock was very low, so that the livestock could not absorb enough nutrients.

In addition, since the toxin called phytin, which is a storage form of phosphorus, exists in the grains of the compound feed, phytin is decomposed into phytic acid by the action of gastric acid to form essential essential minerals and phytate salts in the small intestine.

In addition, it is known that microbes are used to decompose and feed the grains to feed the livestock so that the livestock can digest sufficiently. However, this is merely a function to enhance the physiological activity of the livestock, to promote growth and to improve the feed efficiency. It is difficult to expect effects such as intestinal digestion function and immunity enhancement of the intestines.

In order to solve this problem, Korean Patent Publication No. 2009-0070631 discloses a process for producing a ginseng root, rice bran, glucose, zinc, and the like in a herbal raw material such as ginseng, white bamboo, A fermented feed prepared by mixing a fermented feed prepared by aging fermentation and aging using a microorganism, a calcium feed, a calcium meal and a fish meal, and mixing the fermented feed with a general formula at an appropriate ratio is disclosed, but the cost is high, Diarrhea, constipation, abnormal fermentation, and intestinal abnormalities such as anti-illness, weakness of the face and the quality of the resulting fattening problems.

Accordingly, the technical problem to be solved by the present invention is to improve various symptoms such as constipation, dilatation, northern bloating and intestinal fermentation, thereby preventing diarrhea, improving digestibility, improving appetite, improving feed efficiency, improving quality of meat, And a method for producing the fermented feed.

In order to solve the above-mentioned technical problem, the present invention provides a method for preparing a functional water, comprising the steps of: preparing a first functional water containing sodium and potassium ions; S2 preparing a second functional water containing zinc and selenium ions; A step S4 of mixing and extracting the functional water and the second functional water, a step S4 of mixing and extracting the beer-drying yeast with the first functional water and the second functional water, adding sugar water to the soybean meal extract and the beer dry yeast extract A step S5 of preparing a culture solution inoculated with Saccharomyces cerevisiae boulardii after sterilization treatment, and a step of mixing the protein solution and the molasses with water, and then the first functional water and the second functional water are added, and the culture solution is mixed and fermented And a step S6 of preparing a fermented feed.

According to an embodiment of the present invention, the first functional water may be added to purified water in the form of sodium metasilicate pentahydrate, sodium borate decahydrate, sodium chloride or potassium salt Carbonate may be added and dissolved.

According to another embodiment of the present invention, it is possible to further dissolve the white sugar in the purified water.

According to another embodiment of the present invention, the second functional water may be obtained by adding and dissolving Zinc Sulphate Heptahydrate or Sodium selenite to purified water.

According to another embodiment of the present invention, the extraction filtration in the step S3 or S4 may be such that the mesh is 1.7 mm in size.

According to still another embodiment of the present invention, the step S6 may further include adding a starch to the fermented feed to perform drying.

According to another embodiment of the present invention, the atmospheric temperature performed in step S3, step S4, or step S7 may be 40 to 45 ° C.

Meanwhile, the present invention provides a fermented feed which is produced by the above-described method.

According to the present invention, the culture solution prepared from the culture composition extracted with mineral water in the front is higher in the number of live bacteria of Saccharomyces, and the resulting fermented feed improves various symptoms such as constipation, dilatation, northern bloating and intestinal fermentation, , Improvement of digestibility, improvement of appetite, improvement of feed efficiency, improvement of quality of fattening, anti-illness and immunity.

1 is a flow chart showing a manufacturing method according to the present invention.

Hereinafter, the present invention will be described in detail.

A method for producing a fermented feed according to the present invention comprises the steps of: preparing a first functional water containing sodium and potassium ions; S2 step of preparing a second functional water containing zinc and selenium ion; A step S4 of mixing and extracting the first functional water and the second functional water, a step S4 of mixing and extracting the beer dry yeast with the first functional water and the second functional water, and a step S4 of adding sugar water to the soybean meal extract and beer dry yeast extract, After step S5 of preparing a culture solution inoculated with Saccharomyces cerevisiae boulardii, the protein solution and the molasses are mixed with water, and then the first functional water and the second functional water are added. The culture solution is mixed and fermented, (S6).

Soybean meal and brewer's yeast, which are protein feed sources, are processed and processed to provide extreme culture growth of Saccharomyces cerevisiae boulardii.

Will be described with reference to Fig. 1 attached hereto.

First, in steps S1 and S2, a step of preparing a first functional water containing sodium and potassium ions and a step of preparing a second functional water containing zinc and selenium ions may be performed. And the first functional water is not particularly limited as long as it contains sodium and potassium ions. It is also possible to add sodium metasilicate pentahydrate, sodium borate decahydrate, sodium benzoate, (Sodium Chloride) or Potassium Carbonate may be added and dissolved.

Herein, it is possible to further dissolve the clarithromycin, which can be used as an osmotic synergist upon extraction, as a stabilizer of minerals, and as a source of microbial energy.

In addition, the second functional water is not particularly limited as long as it contains zinc and selenium ions, but may be prepared by dissolving zinc sulphate heptahydrate or sodium selenite in purified water .

Next, the step S3 is a step of mixing and extracting the soybean meal with the first functional water and the second functional water. In this case, the filtration hole of the filter may have a size of 1 to 2 mm, There may be a problem in that the filtration of the extract is blocked by the filter due to the clogging of the filter material. On the other hand, if the filtration amount exceeds 2 mm, the filtrate may be filtered by the fine particulate matter and the culture liquid may become turbid due to the floating material.

The soybean meal is a substitute for peptone. It contains an amino acid component and contains a large amount of crude protein (on average 35% or more) and can be used as a protein nitrogen source necessary for growth of Saccharomyces cerevisiae boulardii.

Further, when the soybean meal and the first and second functional water are mixed and extracted, it is possible to extract more crude protein and other nutrients than that extracted with hot water at 40 ° C due to the alkaline component of the functional water.

Also, in step S4, the beer-drying yeast is mixed with the first functional water and the second functional water, and the filtration hole of the filter may have a size of 1 to 2 mm. , There may be a problem that the filtrate of the extract is blocked by the filter due to the clogging of the fine particulate matter. On the other hand, if it exceeds 2 mm, the filtrate may be filtered by the fine particulate matter and the culture liquid may become turbid due to the suspended particulate matter.

The beer-dried yeast extract is a water-soluble part of a self-degrading yeast containing a vitamin B complex as a beer-dried yeast extract. It can supply crude protein, vitamins, nitrogen, amino acids, and carbon and can be used as a nutrient source for growth of Saccharomyces cerevisiae boulardii .

In step S5, the soybean meal extract and beer dry yeast extract are added with sugar water, sterilized and then inoculated with Saccharomyces cerevisiae boulardii. The sterilization is performed at 85 ° C. Steam is added to the jacket to raise the temperature to 100 ° C or higher and the internal temperature of the culture medium is set at 85 ° C for at least 30 minutes to sterilize the microorganisms and minimize nutrient destruction.

The Saccharomyces cerevisiae boulardii strain is not particularly limited as a known strain.

In step S6, the protein and molasses are mixed and stirred with water, and then the first functional water and the second functional water are added, and the culture medium is mixed and fermented to prepare a solid medium.

The protein content is a protein source for the growth of Saccharomyces cerevisiae boulardii, and the molasses is a source of carbohydrate for microbial growth. The protein content is lower than that of soybean meal and is a protein source of Saccharomyces cerevisiae boulardii. The content of crude protein is not different, and the saccharomyces cerevisiae boulardii provides the carbohydrate necessary for growth and is cheaper than sugar.

Optionally, step S7 may include adding starch to the fermented feed to effect drying.

The above-mentioned starch accelerates the drying by activating the fermentation of the culture, thereby removing water and inhibiting the invasion or proliferation of other harmful bacteria other than Saccharomyces cerevisiae boulardii.

Meanwhile, the atmospheric temperature performed in step S3, S4, or S7 may be 40 to 45 DEG C, and if the temperature is less than 40 DEG C, there is a problem that the drying time is 5 days or more, On the other hand, if it exceeds 45 캜, Saccharomyces cerevisiae boulardii may die due to high temperature.

On the other hand, the present invention provides a fermented feed produced by the above-described method, wherein the fermented feed and the feed are selected from the group consisting of cereals (for example, crushed or crushed wheat, oats, barley, corn, rice) (For example, feeds based on flour, soybeans and sunflower), animal protein feeds (for example, blood, meat, bone meal and fish meal), sugar and dairy products Components, etc.), and may further include nutritional supplements, digestion and absorption enhancers, growth promoters, and the like.

The fermented feed may be administered alone to the animal or in combination with another fermented feed in an edible carrier. The fermented feed may be used as a top dressing or directly mixed with an animal feed, or in a separate oral formulation , And may be prepared in an immediate release or sustained release formulation, in combination with a pharmaceutically acceptable edible carrier, as is well known in the art, when administered separately from animal feed.

Such edible carriers may be solid or liquid such as corn starch, lactose, sucrose, soy flakes, peanut oil, olive oil, sesame oil and propylene glycol, and when solid carriers are used, the fermented feeds may be tablets, capsules, , Troche or hamburger, or top-dressing in finely divided form, and when a liquid carrier is used, the fermented feed can be a gelatin soft capsule, or a syrup or suspension, emulsion, or solution formulation.

The feed may comprise any protein-containing organic fructose commonly used to meet an animal's dietary needs. These protein-containing flours usually consist mainly of corn, soy flour, or corn / soy flour mix.

In addition, the fermented feed and feed may contain adjuvants such as preservatives, stabilizers, wetting or emulsifying agents, solution promoting agents and the like. The fermented feed can be used by adding to the animal's feed by pouring, spraying or mixing.

The fermented feed of the present invention can be applied to a large number of animal diets including mammals, poultry, and fish, and can be used as the mammals such as pigs, cows, horses, sheep, goats, laboratory animals, (Such as dogs and cats), and the poultry can be used for chicken, turkey, duck, goose, pheasant, and quail, and the fish can be used for trout, but the present invention is not limited thereto.

In addition, the fermented feed can be used in animal medicine, and it can be administered concurrently when the intestinal function is improved (rectal function), diarrhea treatment, antibiotics, etc., and can be applied to a large number of animal diets including mammals, poultry and fish .

Example

Mineral water A containing sodium and potassium ions and mineral water B containing zinc and selenium ions were prepared. One liter of soybean extract, 50 g of bean extract, 60 g of beer dry yeast extract and 10 g of sugar were added to the medium, (1.0E + 07 CFU / g) of Saccharomyces cerevisiae boulardii was inoculated into 1,000 ml of the medium sterilized at 121 ° C for 20 minutes and then cultured at 25 ° C for 48 hours. After the incubation, 1 mL of each solution was diluted with the decimal system, and the resultant was subjected to the smear test and cultured in an incubator at 25 ° C. for 48 hours.

Comparative Example

After injecting 2.5 g (1.0E + 07 CFU / g) of Saccharomyces cerevisiae boulardii into 1,000 ml of the medium sterilized at 121 ° C for 20 minutes in a high pressure steam sterilizer after putting 41 g of YM Broth commercial medium of BD Co. into a 1 L injection water, Lt; 0 > C for 48 hours. After the incubation, 1 mL of each solution was diluted with the decimal system, and the resultant was subjected to the smear test and cultured in an incubator at 25 ° C. for 48 hours.

Experimental Example

To compare the viable cell numbers of Saccharomyces cerevisiae boulardii according to the medium, the media according to the present invention and the comparative examples were compared and the results are shown in Table 1 below.

division cell counts (CFU / mL) Comparative Example 1.00E + 7 Example 2.00E + 9

Referring to Table 1, it is analyzed that the embodiment according to the present invention is grown by about 200 CFU / mL more than the commercially available medium commercially available.

Claims (8)

delete delete delete Preparing a first functional water containing sodium and potassium ions;
Preparing a second functional water containing zinc and selenium ions;
Extracting the soybean meal extract by mixing the soybean meal with the first functional water and the second functional water;
(S4) mixing the beer dry yeast with the first functional water and the second functional water separately to extract beer dry yeast extract;
Adding sugar water to the soybean meal extract and beer dry yeast extract, raising the temperature to 100 ° C or higher with steam, sterilizing the internal temperature of the culture medium at 85 ° C for 30 minutes or more, and then preparing a culture solution inoculated with Saccharomyces cerevisiae boulardii;
Mixing the protein solution and the molasses with water, stirring the mixture, adding the first functional water and the second functional water separately, and fermenting the mixture to prepare a fermented feed; And
In step S6, step S7 is performed in which the fermented feed is added with a starch to dry the fermented feed,
The first functional water may be prepared by dissolving Sodium metasilicate pentahydrate, Sodium Borate Decahydrate, Sodium Chloride or Potassium Carbonate into purified water, Is further dissolved,
The second functional water is obtained by adding zinc sulfate heptahydrate or sodium selenite to purified water,
Wherein the atmospheric temperature in step S3, step S4 or step S7 is 40 to 45 占 폚.
5. The method of claim 4,
Wherein the extractive filtration in step S3 or S4 comprises a mesh size of 1.7 mm.
delete delete A fermented feed produced by the method of any one of claims 4 to 5.

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