KR102292878B1 - A method of breeding white grubs using ginseng and a food composition comprising white grubs raised thereof - Google Patents

Abstract

The present invention includes a breeding method of feeding ginseng or red ginseng to the slugs in the primary breeding step, defecation induction phase and the secondary breeding step, and includes slugs that are bred by the breeding method and contain functional ingredients of ginseng or red ginseng. It includes a food and pharmaceutical composition.

Description

{A method of breeding white grubs using ginseng and a food composition comprising white grubs raised thereof}

The present invention relates to a slug breeding method using ginseng and a food composition comprising slugs raised using the same, and more particularly, to a slug breeding method in which a food ingredient containing a functional ingredient is fed and a slug breeding method using the same It relates to a food composition comprising.

Edible insects are in the spotlight as future food amid the approaching food crisis, and in 2013, the Food and Agriculture Organization of the United Nations (FAO) designated brown mealworm, a type of beetle, as a future food resource. Edible insects are a high-protein, low-fat resource that can nutritionally replace meat and seafood, and have the advantage that they can be produced in a narrow breeding space due to their small size and short lifespan, and do not require a lot of cost for production, compared to general livestock. It has the advantage of being able to reduce greenhouse gases by emitting less ammonia and can be reared while feeding organic waste.

Looking at the size of the global edible insect market (edible insect market: global industry analysis and outlook for 2016-2024, Persistence Market Research, 2016), it was about $ 420 million (KRW 500 billion) in 2016, but in 2024 It is expected to expand to $720 million and will continue to grow at an average annual rate of more than 6%.

In Korea, the Ministry of Food and Drug Safety recognizes 7 types of insects as edible insects: grasshopper, silkworm pupa, white silkworm, brown larvae, white-spotted beetle larva, long-leaved beetle larva, and cricket.

A slug refers to a larva that appears in insects that undergo metamorphosis, and the slug that can be used as an edible insect corresponds to the larva of the white-spotted plant. In 2015, the Ministry of Agriculture, Food and Rural Affairs held a contest for a new name for the larva of the white-spotted flower larva, and named it ‘flower bud’, which is a combination of the ‘flower’ of the white-spotted flower larva and the ‘bengi’ of the slug. Looking at Heo Jun's Donguibogam decoction, "Slugs are slightly cold in nature, salty in taste, and poisonous. It treats bloody blood, eohyeol, bigi, fat in the eyes, white membranes, broken bones or sprains, and clogs caused by metal fittings, and makes milk flow well.” In addition, the Korean Pharmacopoeia specifies that slugs contain protesin, a natural antibiotic protein, which helps to improve tonicity and liver function. In the herbal medicine, it is written that it has excellent effects on liver disease, loss of vision, boils, and adult diseases.

As such, as a prior art related to food materials using slugs, there is Korean Patent Registration No. 1953097. The prior art discloses a technique for feeding red ginseng during a fasting period after breeding slugs so that the slugs contain the active ingredient of red ginseng in the body, and manufacturing functional food using the same. However, since red ginseng is provided in powder form, the amount of red ginseng intake may be different for each individual slug, and the absorption rate of active ingredients may be low. Also, when red ginseng is fed, grain powder and pumpkin are mixed and provided, so excrement may remain in the body of slugs.

Another prior art is Korean Patent No. 1716763. The prior art discloses a breeding technique for feeding ginseng according to the growth stage to crickets in order to produce crickets containing ginsenoside-based saponins and ginseng polysaccharide. However, it may be difficult to further include the functional component content of ginseng in the body of crickets only by mixing ginseng with general feed during the growth stage.

Therefore, there is a great need for a breeding method capable of producing slugs that further include the functional component content of ginseng and have no off-flavor and a food composition prepared from the slugs.

Therefore, the first problem to be solved by the present invention is to provide a method for breeding slugs in which the content of functional ingredients is increased by feeding ginseng or red ginseng.

A second problem to be solved by the present invention is to provide a food composition comprising slugs having an increased content of functional ingredients.

In order to achieve the first object, the present invention comprises the steps of squeezing a functional food to produce an extract and by-products and a primary breeding step of feeding the slugs with a feed containing the extract and sawdust, including the extract It provides a slug breeding method comprising a defecation induction step of feeding a fasting composition to the primary reared slugs and a secondary breeding step of feeding a feed containing the by-product to the defecation-induced slugs.

According to one embodiment of the present invention, the functional material may be ginseng or red ginseng.

According to another embodiment of the present invention, the feed containing the extract and sawdust may be a fermented mixture containing the extract and the sawdust.

According to another embodiment of the present invention, the fasting composition comprising the extract may be a fermented extract.

According to another embodiment of the present invention, the feed including the by-product may be fermented after pulverizing the by-product.

In order to achieve the second object, the present invention provides a food composition comprising the slugs and bred slugs having an increased content of functional ingredients using the breeding method.

The method for breeding slugs using ginseng or red ginseng of the present invention and a food composition comprising slugs bred using the same have the following effects.

1. It is possible to further increase the content of functional ingredients of ginseng or red ginseng in the body of the slugs by executing the first breeding step and the defecation induction step, and then feeding the slugs a feed containing ginseng or red ginseng by-products in the secondary breeding stage, It can reduce the odor of slugs products.

2. In the primary breeding stage, by feeding the slugs a fermented feed containing ginseng or red ginseng extract and sawdust, the biomass of slugs can be increased, mortality can be reduced, and the intake rate of ginseng or red ginseng can be increased can do it

3. A food composition containing functional ingredients of ginseng or red ginseng can be prepared by using the slugs raised by the above breeding method.

Hereinafter, the present invention will be described in more detail.

The advantage of the slug breeding method using ginseng or red ginseng of the present invention is that it is possible to further increase the content of functional ingredients of ginseng or red ginseng in the body of the slug, and to reduce the smell of the slug product. A characteristic configuration of the present invention for this is to first execute the primary breeding step and defecation induction step, and then feed the slugs with a feed containing ginseng or red ginseng by-products in the secondary breeding step. Another advantage of the slug breeding method using ginseng or red ginseng of the present invention is that the biomass of slugs can be increased, the mortality rate can be reduced, and the intake rate of ginseng or red ginseng can be increased. A characteristic configuration of the present invention for this purpose is to feed the slugs a feed fermented with a mixture containing ginseng or red ginseng extract and sawdust in the primary breeding stage.

The advantage of the food composition containing slugs of the present invention is that it contains functional ingredients of ginseng and red ginseng. A characteristic configuration of the present invention for this purpose is to use slugs raised by feeding a feed containing ginseng or red ginseng and a fasting composition as an active ingredient in the food composition.

The method for rearing slugs and the food composition using ginseng or red ginseng of the present invention will be described in detail as follows.

The method for rearing slugs using ginseng or red ginseng of the present invention includes the step of squeezing a functional food to produce an extract and a by-product. Since squeezing uses the principle of pressing and turning at the same time like a millstone, when squeezing functional ingredients, the extract and by-products of functional ingredients are separated and produced. When the functional food is a plant, since the cell wall is destroyed by the juice, the functional ingredients in the cell fluid surrounded by the cell wall can be easily obtained in the form of an extract, and the functional ingredients remaining in the cell wall can be obtained as a by-product. Therefore, when the extract and by-products of the functional food produced by squeezing in the above step are fed to the slugs, energy can be minimized in the process of digesting the functional food and the functional ingredients can be rapidly absorbed into the body of the slugs.

In the method for breeding slugs using ginseng or red ginseng of the present invention, the functional food may be ginseng or red ginseng. A method of producing an extract and by-products by squeezing the ginseng or red ginseng is as follows. First, 100 parts by weight of ginseng or red ginseng and 100 parts by weight of water relative to 100 parts by weight of the ginseng or red ginseng are put into a juicer to perform juice extraction. After the juicing is finished, the ginseng or red ginseng is separated into an extract and a by-product. The ginseng or red ginseng extract is then stored in a storage container until used as feed in the primary breeding stage and as a fasting composition in the bowel induction stage. The ginseng or red ginseng by-product is then dried in hot air at 70° C. for 8 hours to be used as feed in the secondary breeding step, and pulverized to a size of 0.5 mm in diameter using a grinder to prepare ginseng or red ginseng by-product powder.

The method for rearing slugs using ginseng or red ginseng of the present invention includes a primary breeding step of feeding the slugs with a feed containing the ginseng or red ginseng extract and sawdust. The first breeding step may be carried out for two weeks. Since ginseng or red ginseng has a unique taste and flavor, slugs tend to avoid it. Therefore, when ginseng or red ginseng is mixed with sawdust in a powder state, it is highly likely that slugs will only pick and eat sawdust. In this case, the functional ingredient of ginseng or red ginseng may be low in the body of the slugs, and the variation in the content of the functional ingredients of ginseng or red ginseng for each individual slug may increase due to the difference in intake. In order to prevent this, the present invention feeds slugs a feed mixed with ginseng or red ginseng extract and sawdust through the primary breeding step. As a result, the slugs can consume ginseng or red ginseng evenly, and the functional ingredients of ginseng or red ginseng can be further increased in the slugs body, and the slugs are adapted to the taste and aroma of ginseng or red ginseng, so that the slugs are adapted to the next defecation induction stage or second stage Even if only ginseng or red ginseng is fed as food in the breeding stage, it is possible to lower the objection to food.

In the slug breeding method using ginseng or red ginseng of the present invention, the feed containing the ginseng or red ginseng extract and sawdust may be prepared by using a method of fermenting a mixture containing the ginseng or red ginseng extract and sawdust. First, a method for preparing a probiotic to be used for fermentation is as follows. 20g of wheat flour is added to 1L of water, uniformly mixed, sterilized at 120°C for 60 minutes, and then cooled to 20°C to prepare a culture solution. And 100 parts by weight of the culture solution is inoculated with 2 parts by weight of microorganisms of the genus Lactobacillus relative to 100 parts by weight of the culture solution, put in a sealed container, and then anaerobically cultured at 25° C. to 35° C. for 5 days to prepare a probiotic. Next, in 100 parts by weight of the ginseng or red ginseng extract produced in the step of squeezing the ginseng or red ginseng to produce the extract and by-product, 300 parts by weight of oak sawdust for slug feed purchased from the market compared to 100 parts by weight of the ginseng or red ginseng extract and A mixture is prepared by adding 4 parts by weight of a probiotic. The mixture is then sealed and fermented at 25° C. to 35° C. for 3 to 4 weeks. After the fermentation is finished, if hot air dried at 30° C. for 6 hours, a feed obtained by fermenting a mixture containing ginseng or red ginseng extract and sawdust is prepared.

The method for breeding slugs using ginseng or red ginseng of the present invention includes a defecation induction step of feeding the fasting composition containing the ginseng or red ginseng extract to the primary bred slugs. The defecation induction step may be performed for 1 week. Since slugs consume sawdust, it is necessary to stop the breeding stage of feeding and induce defecation in order to remove the peculiar smell. However, there is a high possibility that the slugs die if they fast or feed only water or grain flour in the defecation induction step. In order to prevent this, the present invention feeds a fasting composition containing ginseng or red ginseng extract to the primary reared slugs through the defecation induction step. As a result, the slugs can consume nutrients contained in ginseng or red ginseng extract, thereby reducing the possibility of death. In addition, since ginseng or red ginseng extract is ingested, it is possible to prevent a decrease in loss of functional components of ginseng or red ginseng due to defecation.

In the method for breeding slugs using ginseng or red ginseng of the present invention, the fasting composition comprising the ginseng or red ginseng extract may be prepared by using a method of fermenting the ginseng or red ginseng extract. First, to 100 parts by weight of the ginseng or red ginseng extract produced in the step of squeezing the ginseng or red ginseng to produce the extract and by-product, 300 parts by weight of water and 4 parts by weight of the probiotic compared to 100 parts by weight of the ginseng or red ginseng extract is added to the mixture to manufacture Thereafter, when the mixture is sealed and stored at 25° C. to 35° C. and fermented for 3 to 4 weeks, a fasting composition obtained by fermenting ginseng or red ginseng extract is prepared.

The method for rearing slugs using ginseng or red ginseng of the present invention includes a secondary breeding step of feeding the feed containing the ginseng or red ginseng by-product to the defecation-induced slugs. The secondary breeding step may be carried out for two weeks. In the first breeding step, since ginseng or red ginseng is mixed with sawdust and fed to the slugs, the content of functional components of ginseng or red ginseng in the body of the slugs may be small, and the size of the slugs may be small. In addition, since the defecation induction step was performed, the population of slugs may be reduced. In order to prevent this, the present invention feeds a feed prepared from ginseng or red ginseng by-product through the secondary breeding step to the defecation-induced slugs. Accordingly, it is possible to further increase the functional ingredients of ginseng or red ginseng in the body of the slug, and the slug can be grown to a size that can be commercialized in the future.

In the slug breeding method using ginseng or red ginseng of the present invention, the feed including the ginseng or red ginseng by-product may be prepared by using a method of fermenting a powder obtained by pulverizing the ginseng or red ginseng by-product. First, to 100 parts by weight of the ginseng or red ginseng by-product powder produced in the step of squeezing the ginseng or red ginseng to produce the extract and by-product, 1 part by weight of the probiotic compared to 100 parts by weight of the ginseng or red ginseng by-product powder is added to prepare a mixture . The mixture is then sealed and fermented at 25° C. to 35° C. for 3 to 4 weeks. After the fermentation is finished, if hot air dried at 30° C. for 6 hours, a feed obtained by fermenting ginseng or red ginseng by-product powder is prepared.

The food composition containing slugs of the present invention aims to provide a food composition containing functional ingredients of ginseng or red ginseng using slugs raised by feeding a feed and a fasting composition containing ginseng or red ginseng. . The method for preparing the food composition is as follows. First, feed and fasting composition containing ginseng or red ginseng are fed in the breeding step and defecation induction step to select the bred slugs. Next, after washing the selected slugs, the slugs are dried using a hot air dryer or a freeze dryer. The dried slugs are pulverized or extracted to prepare a food composition in the form of a powder or an extract. The food composition may include food, health supplements and feed.

Hereinafter, the present invention will be described in more detail using Preparation Examples, Examples, Comparative Examples, and Experimental Examples.

Preparation Example 1: Preparation of ginseng extract

100 parts by weight of ginseng and 100 parts by weight of water relative to 100 parts by weight of ginseng were put into a juicer and juiced to prepare a ginseng extract.

Preparation Example 2: Preparation of ginseng by-product powder

After preparing the ginseng extract in Preparation Example 1, the ginseng by-product remaining in the juicer was taken out and dried with hot air at 70° C. for 8 hours. Thereafter, it was ground to a size of 0.5 mm in diameter to prepare a ginseng by-product powder.

Preparation Example 3: Preparation of ginseng powder

Ginseng powder was prepared by drying ginseng with hot air at 70° C. for 8 hours and then pulverizing it to a size of 0.5 mm in diameter.

Preparation Example 4: Preparation of probiotics

20 g of wheat flour was added to 1 L of water, mixed uniformly, sterilized at 120° C. for 60 minutes, and cooled to 20° C. to prepare a culture solution. To 100 parts by weight of the culture solution, 2 parts by weight of microorganisms of the genus Lactobacillus relative to 100 parts by weight of the culture solution were inoculated, put in a sealed container, and then anaerobically cultured at 25° C. to 35° C. for 5 days to prepare a probiotic.

Preparation Example 5: Preparation of mixed fermented feed with ginseng extract and sawdust

To 100 parts by weight of the ginseng extract of Preparation Example 1, 300 parts by weight of oak sawdust for slug feed purchased in the market and 4 parts by weight of the probiotic of Preparation Example 4 were added to 100 parts by weight of the ginseng extract to prepare a mixture. The mixture was then sealed and fermented at 25°C to 35°C for 3 to 4 weeks. After the fermentation was completed, hot air drying was performed at 30° C. for 6 hours to prepare a mixed fermented feed of ginseng extract and sawdust.

Preparation Example 6: Preparation of ginseng extract fermentation fasting composition

To 100 parts by weight of the ginseng extract of Preparation Example 1, 300 parts by weight of water and 4 parts by weight of the probiotic of Preparation Example 4 were added to 100 parts by weight of the ginseng extract to prepare a mixture. Thereafter, the mixture was sealed and stored and then fermented at 25° C. to 35° C. for 3 to 4 weeks to prepare a composition for fasting fermentation of ginseng extract.

Preparation Example 7: Preparation of ginseng by-product powder fermented feed

To 100 parts by weight of the ginseng by-product powder of Preparation Example 2, 1 part by weight of the probiotic of Preparation Example 4 was added to 100 parts by weight of the ginseng by-product powder to prepare a mixture. The mixture was then sealed and fermented at 25°C to 35°C for 3 to 4 weeks. After the fermentation was completed, hot air drying was performed at 30° C. for 6 hours to prepare a fermented ginseng by-product powder feed.

Example 1: Primary breeding stage of feeding ginseng extract and sawdust mixed fermented feed

A primary breeding step was performed in which the slugs were fed the ginseng extract and sawdust mixed fermented feed of Preparation Example 5 for 2 weeks.

Example 2: Defecation induction step of feeding ginseng extract fermentation fasting composition

A bowel movement induction step of feeding the ginseng extract fermentation fasting composition of Preparation Example 6 to the slugs that had undergone the primary breeding step of Example 1 for 1 week was performed.

Example 3: Secondary breeding step of feeding ginseng by-product powder fermented feed

A secondary breeding step of feeding the ginseng by-product powder fermented feed of Preparation Example 7 to the slugs subjected to the defecation induction step of Example 2 was performed for 2 weeks.

Example 4: Primary breeding step of feeding ginseng extract and fermented sawdust mixed feed

A mixed feed of ginseng extract and fermented sawdust was prepared by mixing 100 parts by weight of the ginseng extract of Preparation Example 1 and 300 parts by weight of fermented oak sawdust for slug feed purchased in the market compared to 100 parts by weight of the ginseng extract. After the preparation, a primary breeding step of feeding the slugs with the ginseng extract and fermented sawdust mixed feed for 2 weeks was performed. Thereafter, the defecation induction step and the secondary breeding step were performed in the same manner as in Examples 2 and 3 above.

Example 5: Primary breeding stage of feeding ginseng extract and sawdust mixed feed

With 100 parts by weight of the ginseng extract of Preparation Example 1, 300 parts by weight of oak sawdust for slug feed purchased in the market compared to 100 parts by weight of the ginseng extract was mixed to prepare a ginseng extract and sawdust mixed feed. It was carried out in the same way.

Example 6: Primary breeding stage of feeding ginseng powder and sawdust mixed feed

With 100 parts by weight of the ginseng powder of Preparation Example 3, 100 parts by weight of the commercially purchased oak sawdust for slug feed compared to 100 parts by weight of the ginseng powder was mixed to prepare a mixed feed of ginseng powder and sawdust. It was carried out in the same way.

Example 7: defecation induction step of feeding ginseng extract

The defecation induction step of feeding the ginseng extract of Preparation Example 1 to the slugs that had undergone the primary breeding step of Example 1 for 1 week was performed. Thereafter, the secondary breeding step was carried out in the same manner as in Example 3.

Example 8: fasting defecation induction step

It was carried out in the same manner as in Example 3, except that the slugs that had undergone the primary breeding step of Example 1 were fasted for 1 week.

Example 9: defecation induction step of feeding water

It was carried out in the same manner as in Example 3, except that water was fed to the slugs that had undergone the primary breeding step of Example 1 for 1 week.

Example 10: Secondary breeding step of feeding ginseng by-product powder

A secondary breeding step of feeding the ginseng by-product powder of Preparation Example 2 to the slugs subjected to the defecation induction step of Example 2 was performed for 2 weeks.

Example 11: Secondary breeding step of feeding ginseng by-product powder and sawdust mixed feed

100 parts by weight of the ginseng by-product powder of Preparation Example 2 was mixed with 100 parts by weight of the ginseng by-product powder compared to 100 parts by weight of the commercially purchased oak sawdust for slug feed to prepare a mixed feed of ginseng by-product powder and sawdust. After the preparation, a secondary breeding step of feeding the ginseng by-product powder and sawdust mixed feed to the slugs subjected to the defecation induction step of Example 2 was performed for 2 weeks.

Comparative Example 1: Breeding step of feeding sawdust feed

A breeding stage was performed in which the slugs were fed with oak sawdust for slug feed purchased in the market for 4 weeks.

Comparative Example 2: Defecation induction step of feeding ginseng powder

A bowel movement induction step of feeding the ginseng powder of Preparation Example 3 to the slugs that had undergone the breeding step of Comparative Example 1 for 1 week was performed.

Comparative Example 3: Breeding step of feeding ginseng powder and sawdust mixed feed

A breeding step was performed in which the slugs were fed the mixed feed of ginseng powder and sawdust prepared in Example 6 for 4 weeks.

Comparative Example 4: Fasting defecation induction step

A bowel movement induction step was performed in which the slugs subjected to the breeding step of Comparative Example 3 were fasted for 1 week.

Experimental Example 1: Evaluation of detection of ginsenosides Rg1, Rb1 and Rg3 in the body of slugs

The slugs of Examples 3 to 11 and the slugs of Comparative Examples 2 and 4 were freeze-dried at a temperature of -30°C and then pulverized to a diameter of 0.5 mm to prepare a slug powder. After adding 70% methanol in 10 times the weight of the slug powder, the mixture was extracted three times by reflux in a constant temperature water bath at 80° C. and dried under reduced pressure. After adding water to the dried product, diethyl ether was added to remove the fat-soluble component, extracted three times with saturated n-butanol, concentrated under reduced pressure, and treated with methanol as the final injection solvent. The detection of ginsenosides Rg1, Rb1 and Rg3 was evaluated using a reversed-phase column (C18) and a UV detector (PerkinElmer Series 200 HPLC, 203 nm), and the results are shown in Table 1 below.

Referring to Table 1 below, it can be seen that ginsenoside was detected the most in Example 3, and fermented ginseng extract or ginseng by-product in the first breeding step, defecation induction step, and the second breeding step to feed the slugs. It can be seen that the amount of ginsenoside detected is large.

Rg1 (mg/g) Rb1 (mg/g) Rg3 (mg/g) Example 3 0.80 1.12 0.08 Example 4 0.51 0.70 0.04 Example 5 0.50 0.71 0.04 Example 6 0.42 0.60 0.03 Example 7 0.49 0.69 0.05 Example 8 0.41 0.61 0.03 Example 9 0.40 0.60 0.03 Example 10 0.51 0.68 0.04 Example 11 0.40 0.59 0.03 Comparative Example 2 0.10 0.11 0.01 Comparative Example 4 0.09 0.12 0.01

Experimental Example 2: Evaluation of taste and preference

The slugs of Examples 3 to 11 and the slugs of Comparative Examples 2 and 4 were freeze-dried under a temperature condition of -30°C and then pulverized to a size of 0.5 mm in diameter to prepare a slug powder. The slug powder was diluted 10-fold in purified water to prepare 100 ml of each beverage. Thirty men and women were randomly selected to taste the beverage, and flavor, aroma, color, taste and overall preference were evaluated. The results are shown in Table 2 below. The evaluation was carried out using a 9-point scale method, and a score of 1 point was given when the evaluation of each item corresponds to very bad, and 9 points when the evaluation of each item corresponds to very good. (Evaluation unit: points)

Referring to Table 2 below, in Example 3, the smell was the lowest, and the preference was the highest. In addition, the slugs of Examples 3 to 10 had a short period of ingesting sawdust feed, and consumed ginseng by-products after the defecation induction stage. .

zest incense color taste Comprehensive symbology Example 3 9.5 9.5 9.4 9.8 9.5 Example 4 8.5 8.3 8.4 8.7 8.5 Example 5 8.3 8.4 8.1 8.2 8.2 Example 6 8.0 8.1 8.1 8.0 8.1 Example 7 9.2 9.1 9.0 9.1 9.1 Example 8 9.0 9.1 9.0 9.0 9.0 Example 9 9.1 9.0 9.1 9.2 9.1 Example 10 8.9 9.0 8.8 9.7 8.9 Example 11 7.6 7.3 7.2 7.5 7.4 Comparative Example 2 6.4 6.1 6.2 6.2 6.3 Comparative Example 4 6.0 6.2 6.1 6.1 6.0

Experimental Example 3: Evaluation of individual biomass and mortality

The slugs of Examples 3 to 11 and the slugs of Comparative Examples 2 and 4 were randomly selected 20 each from a group of 100, and the weights of the slugs were measured at intervals of one week during breeding to obtain an average value. The unit of each weight was g. In addition, the mortality rate was calculated by counting the slugs remaining in the group of 100 slugs after all breeding and defecation induction periods were completed, and the results are shown in Table 3 below.

Referring to Table 3 below, the biomass of the slugs of Example 3 was the highest, and the mortality rate was the lowest. In addition, the slugs of Examples 3 to 7, 10, and 11 fed ginseng extract or ginseng extract fermentation fasting composition in the defecation induction step had a lower mortality rate than the slugs of Examples 8 and 9 and the slugs of Comparative Examples 2 and 4 appeared to be

1 week (g) 2 weeks (g) 3 weeks (g) 4 weeks (g) 5 weeks (g) Mortality (%) Example 3 0.40 1.98 1.97 3.04 5.12 98 Example 4 0.41 1.54 1.52 2.82 3.94 90 Example 5 0.40 1.53 1.51 2.79 3.89 89 Example 6 0.42 1.50 1.49 2.34 3.10 82 Example 7 0.41 1.55 1.54 2.80 3.95 90 Example 8 0.41 1.46 1.19 2.10 3.00 75 Example 9 0.41 1.45 1.20 2.13 3.05 79 Example 10 0.41 1.50 1.48 2.41 3.12 81 Example 11 0.40 1.49 1.47 2.39 3.11 82 Comparative Example 2 0.41 1.54 2.75 4.15 3.10 68 Comparative Example 4 0.41 1.60 2.90 4.18 3.15 62

Experimental Example 4: Evaluation of ginseng feed preference

The preference of the ginseng-containing feed provided to the slugs of Examples 3 to 7, 10 and 11 and the slugs of Comparative Examples 2 and 4 was evaluated. In order to evaluate the preference of the feed containing ginseng provided during the primary breeding, the feed intake rates of the slugs of Examples 3 to 6 were compared. And in order to evaluate the preference of the fasting composition containing ginseng provided during induction of defecation, the intake rate of the fasting composition of the slugs of Examples 3 and 7 and Comparative Example 2 was compared. In addition, in order to evaluate the preference of the feed containing ginseng provided during the secondary breeding, the feed intake rates of the slugs of Examples 3, 10 and 11 were compared. The unit of each intake rate was %, and the results are shown in Table 4 below.

Referring to Table 4 below, it was found that the slugs of Example 3 had the highest intake rates in all of the samples of the primary breeding stage, the fasting composition of the defecation induction stage, and the feed of the secondary breeding stage. In addition, it was found that when the feed and fasting composition containing ginseng were fermented, the bitter taste was alleviated and the taste was softened, and the feed intake rate of slugs was improved. In the case of the slugs of Comparative Example 2, it was found that the intake rate of the fasting composition was very low due to the increase in rejection because ginseng powder was consumed in the bowel movement induction stage after only consuming sawdust.

Feed intake rate in the first breeding stage (%) Example 3 90 Example 4 75 Example 5 70 Example 6 68 Intake rate of fasting composition for bowel induction phase (%) Example 3 98 Example 7 57 Comparative Example 2 12 Feed intake rate in the second breeding stage (%) Example 3 95 Example 10 78 Example 11 80

The above description describes the technical idea of the present invention using one embodiment, and various modifications and variations will be possible without departing from the essential characteristics of the present invention by those of ordinary skill in the art to which the present invention pertains. . Accordingly, the embodiments described in the present invention are for explanation rather than limiting the technical spirit of the present invention, and the scope of the technical spirit of the present invention is not limited by these embodiments. The protection scope of the present invention should be construed by the claims, and all technical ideas within the scope equivalent thereto should be construed as being included in the scope of the present invention.

Claims (6)

Juicing ginseng or red ginseng to produce an extract and by-products;
A primary breeding step of feeding the feed containing the extract and sawdust to the slugs;
Defecation induction step of feeding the fasting composition containing the extract to the slugs; and
A method of breeding slugs comprising a secondary breeding step of feeding the feed containing the by-product to the slugs. delete The method according to claim 1,
The feed containing the extract and sawdust is a slug breeding method that is fermented with a mixture containing the extract and the sawdust. The method according to claim 1,
The fasting composition comprising the extract is a slug breeding method by fermenting the extract. The method according to claim 1,
The feed containing the by-product is a slug breeding method that is fermented with the pulverized powder of the by-product. delete