KR102474576B1 - Eco-friendly feed composition containing salad vegetable by-products and lactic acid bacteria - Google Patents

Abstract

The present invention relates to an eco-friendly feed composition comprising salad vegetable by-products and lactic acid bacteria. The feed composition of the present invention can improve the growth of animals that ingest the feed composition, as well as exhibit an immune enhancing effect. The eco-friendly feed composition further comprises 5 to 10 parts by weight of Siegesbeckia glabrescens extract, 5 to 10 parts by weight of Potentilla supina L. extract, and 5 to 10 parts by weight of Serratula coronata L. extract, with respect to 100 parts by weight of the salad vegetable by-products.

Description

Eco-friendly feed composition containing salad vegetable by-products and lactic acid bacteria {Eco-friendly feed composition containing salad vegetable by-products and lactic acid bacteria}

The present invention relates to an eco-friendly feed composition containing salad vegetable by-products and lactic acid bacteria. More specifically, it relates to an eco-friendly feed composition that includes natural materials and lactic acid bacteria as active ingredients and can improve growth and immunity enhancement effects of animals ingesting them.

Recently, as consumers' income increases, leisure activities increase, and interest in health increases, products containing functional substances with physiologically active effects are emerging, and in livestock products, production using functional feed products and consumption of meat products need is emerging.

In the livestock industry, production efficiency has been remarkably improved due to genetic improvement of animals and improvement in the development of excellent feed, but various metabolic diseases of animals are rather increasing, which is a major obstacle to the competitiveness of the livestock industry. On the other hand, as the production of livestock is commercialized as a mass breeding production system in a small space, various diseases associated with increased stress and immunodeficiency are increasing among animals. In addition, the increase in the amount of synthetic feed is also acting as a cause of various diseases together with the decrease in immunity of animals.

In order to prevent and treat these diseases, livestock farmers have been overusing antibiotics, and accordingly, problems such as the emergence of antibiotic-resistant strains and the retention of antibiotics have emerged as social issues.

The use of growth-promoting antibiotics in livestock feed is aimed at maximizing livestock productivity, preventing diseases caused by high-density breeding and poor breeding environments. However, regulations on the use of growth-promoting antibiotics are being strengthened as problems such as antibiotic retention and emergence of resistant bacteria in livestock products emerge. Interest is growing. However, when breeding without antibiotics, there is a concern that problems such as reduced livestock productivity and increased disease incidence may occur, and countermeasures are urgently needed.

Specifically, the antibiotics injected into the feed are completely digested and excreted after 2 to 3 months. When shipped and consumed before such a withdrawal period, the residual antibiotics in livestock reduce the immunity of the human body ingesting the meat product. have. In addition, bacteria with resistance to antibiotics can be transferred to other bacteria by the transfer of conjugation of plasmids, which are genetic materials containing resistance, which can act as a very fatal problem for health and hygiene, such as causing the occurrence of Salmonella. can As a result, the increase in these antibiotic-resistant bacteria always poses a risk of metastasis to the human body.

In recent years, as the problem of these antibiotic-resistant bacteria has been raised along with the residual antibiotics in meat products, research on alternative substances for antibiotics has been actively conducted. Research on this is actively progressing.

More specifically, probiotics, acidifiers, enzymes, mineral supplements, and oligosaccharides are representative examples, and recently, animals (Zasloff, 1987; Skerlavaj et al., 1999; Henzler et al., 2003;) and natural antimicrobial peptides (AMPs) extracted from plants (Taniguchi and Kubo, 1993; Broekaert et al., 1995;) have attracted much attention.

Antimicrobial peptides are low-molecular-weight proteins that act on the innate immune defense system and are found in all organisms (Boman and Hultmark, 1987; Boman et al., 1991; Hoffmann, 1995). In addition, antibacterial peptides exhibit a wide range of antibacterial effects, such as bacteria (Gazit et al., 1994; Saberwal and Nagaraj, 1994; Steiner et al., 1998), viruses and fungi (Quang et al., 1992; Lee et al., 1995; De Lucca and Walsh, 1999; Lee et al., 1995). et al., 1999;) and has potential as a new antibiotic substitute by improving immunity. However, despite the immune-enhancing effect of the antimicrobial peptide, the research is insignificant to achieve a sufficient effect by using it as an antibiotic substitute for enhancing immune function.

In addition, various substances using useful substances in plants are used as antibiotic substitutes, but their effects are not satisfactory, so there is a limit to being included as a component of a feed composition and commercialized.

Therefore, the present applicant recognizes the above problems, confirms the effect of improving animal growth and enhancing immunity through the combination of salad vegetable by-products and lactic acid bacteria discarded in the salad manufacturing process, and develops a plan to utilize it as an eco-friendly feed composition. reached

KR 10-2018-0083137 A

An object of the present invention is to provide an eco-friendly feed composition for animals containing salad vegetable by-products and lactic acid bacteria.

Another object of the present invention is to provide an eco-friendly feed composition for animals containing salad vegetable by-products and lactic acid bacteria, which can improve the growth and immunity enhancement effects of animals ingested by including natural materials and lactic acid bacteria as active ingredients.

Another object of the present invention is to provide an animal feed comprising the eco-friendly feed composition.

In order to achieve the above object, an eco-friendly feed composition according to an embodiment of the present invention is to include salad vegetable by-products and lactic acid bacteria as active ingredients.

The feed composition is excellent in improving animal growth and improving immunity.

The animal may be selected from the group comprising cows, pigs, horses, chickens, ducks, chicks, dogs, sheep, cats and rabbits.

The salad vegetable by-product may be selected from the group consisting of celery, red cabbage, mustard greens, chicory, romaine, lettuce, kale, cucumber, onion, broccoli, rucola, asparagus, and mixtures thereof.

The lactic acid bacteria may include bacteria selected from the group consisting of the genus Lactobacillus, the genus Leuconostoc, the genus Weissella, and a mixture thereof.

The composition may further include an additive, and the additive may be selected from the group consisting of sorbitol, sodium phosphate, beta-glucan, protease, citric acid, and mixtures thereof.

An eco-friendly animal feed according to another embodiment of the present invention includes the composition.

Hereinafter, the present invention will be described in more detail.

As used herein, the term 'vegetable by-product' refers to a part of salad vegetables used in the manufacturing process of salad for instant food that is not used as a salad ingredient, or a salad ingredient for instant food due to various reasons such as browning or freshness problems. It means the part that cannot be removed.

Specifically, in the case of leafy vegetables such as celery and cabbage, roots, stems, and fruit parts other than the leaf parts actually used as salad ingredients, and leaf parts that cannot be used as ingredients for salad due to browning, etc. do. In the case of root vegetables such as carrots and sweet potatoes, it is defined as stems, leaves, and fruit parts other than the root parts used as salad ingredients, and root parts that cannot be used as ingredients for salad due to lack of marketability due to freshness. On the other hand, in the case of stem vegetables such as asparagus and celery, it refers to fruits, roots and leaves excluding the stems used as ingredients for salads, and stems that cannot be used for salad due to lack of marketability due to browning, etc. Cucumbers, tomatoes, etc. Even in the case of fruit vegetables, it is defined as meaning the root, stem, and leaf parts other than the fruit part used as a salad ingredient, and the part of the fruit that cannot be used as a salad ingredient because of browning, etc.

The term 'extract' used herein has a meaning commonly used in the art as a crude extract, but also includes a fraction obtained by further fractionating the extract in a broad sense. That is, the plant extract includes not only those obtained using an extraction solvent such as water, lower alcohol, or ether chloroform, but also those obtained by additionally applying a purification process thereto. For example, a fraction obtained by passing the extract through an ultrafiltration membrane having a certain molecular weight cut-off value, separation by various chromatography (made for separation according to size, charge, hydrophobicity or affinity), etc. Fractions obtained through various purification methods are also included in the extract of the present invention.

The extract used in the present invention may be prepared in a powder state by additional processes such as distillation under reduced pressure and freeze drying or spray drying.

As used herein, the term "comprising as an active ingredient" refers to an amount sufficient to affect a desired biological effect, such as beneficial results, including but not limited to prevention, reduction, alleviation or elimination of signs or symptoms of a disease or disorder. , and this amount is referred to as the “effective amount”. Thus, the total amount of each active ingredient in the composition or method is sufficient to yield a significant individual benefit. Thus, the effective amount will depend on the circumstances in which it is being administered. An effective amount can be administered in one or more prophylactic or therapeutic administrations.

Eco-friendly feed composition according to an embodiment of the present invention is to include salad vegetable by-products and lactic acid bacteria as active ingredients.

Salad is a Western food that is eaten by mixing vegetables, fruits and meat products evenly and seasoning them with mayonnaise or dressing.

The word salad is derived from the Latin sal (salt), and it is estimated that Westerners who consume a lot of meat had a habit of sprinkling salt on raw vegetables.

Originally, it is known that garlic, parsley, celery, and watercress (Cresson), which are medicinal herbs, are used as ingredients to help digestion and absorption. Eating acidic meat dishes such as beef steak and roast beef with alkaline raw vegetables gives a refreshing taste, balances nutrients and is effective in absorption, so it is widely used as an essential food for acidic foods. Your choice of sauce can serve to whet your appetite.

On the other hand, as modern people's interest in diet increases, the case of replacing meals with salads and fruits is increasing, and as food processing technology develops, fruits and various vegetables cut into easy-to-eat sizes are used as salad sauce. It is sold in a container and packaged so that consumers can easily eat salad as a meal replacement without going through a special process.

For the above reasons, the demand for "instant food salad products" that provide salad vegetables in a container with dressing (sauce) is increasing, and various food companies are actively researching and commercializing related products. to be.

On the other hand, in the process of manufacturing the salad for instant food, the process of removing the edge part or the browned part with poor marketability among the parts of the salad vegetable used as the main raw material of the salad is necessarily performed, so that the actual instant food The amount of salad vegetables discarded without being used as ingredients for dragon salad is also rapidly increasing day by day.

Accordingly, the present applicant uses salad vegetables (salad vegetable by-products) that are discarded and not used as ingredients for actual instant food salads due to commerciality problems during the manufacturing process of instant food salads as an active ingredient of the feed composition, thereby ingesting them. In addition to being able to exhibit growth and immune enhancing effects in animals that do, it was possible to reduce the amount of salad vegetables to be discarded.

The feed composition is excellent in improving animal growth and improving immunity.

Specifically, in the case of the feed composition of the present invention, each active ingredient of salad vegetable by-products and lactic acid bacteria included as active ingredients can act on animals that consume them, and compared to animals that consume general feeds provided on the market, their growth rate has the potential to improve.

On the other hand, the immune enhancing effect can be confirmed by demonstrating through experiments that the feed composition of the present invention reduces the production of NO (nitric oxide) in LPS (lipopolysaccharide)-induced cells.

NO is an indicator of an inflammatory response and is produced when cells are activated by stimulation of cytokines, invasion of microorganisms, etc., and is produced by nitric oxide synthase (NOS) from L-arginine.

NOS exists in three forms: neuronalNOS (nNOS, NOS1), inducible NOS (iNOS, NOS2), and endothelial NOS (eNOS, NOS3).

On the other hand, LPS (lipopolysaccharide) is a bacterial endotoxin recognized by TLR4 (toll-like receptor 4) among various foreign substances, and is one of the main factors that cause an inflammatory response by activating macrophages. Activated TLR4 activates two major signaling pathways, NF-kB and MAPK.

ERK1/2, JNK, and p38 constituting the MAPKs activate ATF, CREB, STAT3, and NF-kB, which are transcriptional regulators. Among them, the NF-kB is an important transcription factor involved in immune response, apoptosis, and inflammation. It is activated through several steps of signal transduction inside the cell, moves into the nucleus, binds to DNA, and then goes through a transcription step. Expression of inflammatory protein genes such as inflammatory cytokines TNF (tumor necrosis factor)-α, IL (interleukin)-1β and IL (interleukin)-6 and inflammatory proteins COX (cyclooxygenase)-2 and iNOS (inducible nitric oxide synthase) by increasing NO (nitric oxide), PGE ₂ (prostaglandin E ₂ ), etc. are finally produced to mediate the inflammatory response to the inflammatory site.

That is, the composition of the present invention exhibits an immune-enhancing effect through inhibition of NO production, and as a result, can exhibit anti-inflammatory activity.

The animal may be selected from the group comprising cows, pigs, horses, chickens, ducks, chicks, dogs, sheep, cats and rabbits.

Animals capable of exhibiting growth enhancement and immunity enhancement effects through ingestion of the feed composition of the present invention may be selected from the group including cows, pigs, horses, chickens, ducks, chicks, dogs, sheep, cats and rabbits. , It is not limited thereto, and is not particularly limited as long as it is an animal capable of ingesting the feed composition.

The salad vegetable by-product may be selected from the group consisting of celery, red cabbage, mustard greens, chicory, romaine, lettuce, kale, cucumber, onion, broccoli, rucola, asparagus, and mixtures thereof.

However, the vegetable by-products of the present invention are not limited to the types of vegetables exemplified above, and are salad vegetables that can be used as ingredients for salads for instant food. Ji is defined as not particularly limited as long as it is a portion of salad vegetables.

The lactic acid bacteria may include bacteria selected from the group consisting of the genus Lactobacillus, the genus Leuconostoc, the genus Weissella, and a mixture thereof.

The lactic acid bacteria (Lactic acid bacteria) refers to microorganisms that produce lactic acid as a main product as a result of fermentation among bacteria growing by fermentation, and the feed composition of the present invention additionally includes the lactic acid bacteria as a component, so that it is mixed with salad vegetable by-products. It has the advantage of being able to exhibit the effect of enhancing the immunity of animals ingesting the feed composition with a synergistic effect according to.

Lactic acid bacteria are generally Bifidobacterium, Leuconostoc, Pediococcus, Weissella, Streptococcus, Lactobacillus, etc. Contains a variety of fungal species.

Among the lactic acid bacteria described above, in the present invention, lactic acid bacteria selected from the group consisting of the genus Lactobacillus, the genus Leuconostoc, the genus Weissella, and a mixture thereof may be included.

More specifically, the genus Lactobacillus includes Lactobacillus brevis, Lactobacillus acidophilus, Lactobacillus bulgaricus, Lactobacillus plantarum, and lactobacillus. Lactobacillus kimchii, Lactobacillus paraplantarum, Lactobacillus curvatus subsippy. Curvatus (Lactobacillus curvatus subsp. curvatus), Lactobacillus sake subsippi. It is any one selected from the group consisting of sakei (Lactobacillus sakei sibsp. sakei) and mixtures thereof.

The Leuconostoc genus includes Leuconostoc citreum, Leuconostoc lactis, and Leuconostoc mesenteroid subsp. Dextranicum (Leuconostoc mesenteroides subsp. dextranicum), Leuconostoc mesenteroides subsp. Leuconostoc mesenteroides subsp. Mesenteroides, Leuconostoc argentinum, Leuconostoc carnosum, Leuconostoc gellidum, Leuconostoc kimchii ), Leuconostoc inhae, Leuconostoc gasicomitatum, and mixtures thereof.

The Weissella genus includes Weissella koreensi, Weissella hanii, Weissella kimchii, Weissella soli, Weissella confusa And any one selected from the group consisting of mixtures thereof.

More preferably, the feed composition of the present invention is Lactobacillus sakei, Leuconostoc mezenteroid subsp. When included as a complex lactic acid bacteria containing both Leuconostoc mesenteroides subsp. Mesenteroides and Weissella koreensis, synergistic effects according to the mixing of each lactic acid bacteria can maximize the growth and immunity enhancement effects of animals. There are advantages to being able to

More specifically, the lactic acid bacteria are 20 to 30 parts by weight of Lactobacillus sakei based on 100 parts by weight of salad vegetable by-products, Leuconostoc mesenteroid subsp. It may be included in 20 to 30 parts by weight of Leuconostoc mesenteroides subsp. Mesenteroides and 20 to 30 parts by weight of Weissella koreensis, but is not limited thereto.

The composition may further include an additive for improving animal growth and immunity enhancement effects, and the additive may be selected from the group consisting of sorbitol, sodium phosphate, beta-glucan, protease, citric acid, and mixtures thereof.

Specifically, the sorbitol may be added to prevent protein denaturation and control the sweetness and wetness of the feed composition of the present invention, the sodium phosphate may be added to buffer the pH of the feed composition, and the beta glucan It may be added to increase the immunity of animals ingesting the feed composition of the present invention.

On the other hand, the protease (protease) is a proteolytic enzyme, which is widely present in tissues or cells of animals and plants, and microorganisms. It can be roughly divided into exopeptidases and endopeptidases according to the degradation mode. On the other hand, depending on the essential amino acid residues or conditions that act as catalysts, they can be classified into four types: serine enzymes, thiol enzymes, metal enzymes, and acid enzymes. The protease has characteristics required when ingesting amino acids, which are indispensable protein materials for organisms, by decomposing other proteins.

In addition, the citric acid is a weak organic acid commonly called citric acid, and is characterized by being mainly found in tangerines, lemons, and the like in nature. On the other hand, it has a characteristic of exhibiting a sterilization effect, and is widely used as an eco-friendly disinfectant along with baking soda, etc., and also has a characteristic of being used as a sweetener or aromatic agent for food.

Specifically, the additive includes 3 to 7 parts by weight of sorbitol, 3 to 7 parts by weight of sodium phosphate, 3 to 7 parts by weight of beta-glucan, 3 to 7 parts by weight of protease, and 3 to 7 parts by weight of citric acid, based on 100 parts by weight of salad vegetable by-products. It may be included, and within the above range, it may exhibit deterioration prevention of feed, excellent pH buffering effect, and the like.

Preferably, the feed composition of the present invention may further include a natural extract, and the natural extract may include a Jindeukchal extract, an extract of S.

The Jindeukchal (Siegesbeckia glabrescens) is an annual herb belonging to the family Chrysanthemum (Asteraceae; Compositae), and when used for living, it eliminates damp heat and at the same time treats muscle pain or numbness caused by dampness When steamed in yellow wine, it has the effect of removing customs, nourishing the liver and kidneys, and strengthening bones and muscles. It is mainly used for symptoms of paralysis of the limbs, sore knees, sore knees, palpitations, and hemiplegia. In addition, there is a record that it was used for skin diseases such as eczema and tumors, and it is also used as a detoxifying effect for symptoms of snake or insect bites.

The Potentilla supina L. is a perennial plant belonging to the family Primrose, and the small leaves (lobules) of the Potentilla supina have deep sawtooth on the edge, and are also called quills because their shape resembles a pitchfork. In Korea, it grows in fields, roadsides, and clearings all over the country. Stem height is about 20 to 50 cm. The lower part grows obliquely to the side and then stands straight. Leaves face each other and are pinnate compound leaves composed of 2 to 4 pairs of small leaves, with long petioles. Small leaves are obovate, elliptical or lanceolate, 0.7 to 2.5 cm long, 0.8 to 1.4 cm wide, with rounded ends and sawtooth edges. Several yellow flowers with a diameter of 6 to 8 mm hang from May to September in the axils at the ends of branches. The fruit is an achene, ripens in September to October, and is characterized by being hairless. Young stems and leaves are edible, and are distributed in temperate regions of the northern hemisphere including Korea.

The spleen (Serratula coronata L. subsp. insularis Kitamura) is a dicotyledonous perennial plant of the Asteraceae family that grows in the mountains, has a vertical line with a height of 30 to 140 cm, the rhizome is hard like a tree, and the stem is straight. The leaf attached to the root is an egg-shaped long oval with a pointed end and completely split like a feather. The leaves attached to the stem are similar to the leaves attached to the root, but the size gets smaller as you go up. Flowers bloom in July to October in light reddish purple, and head flowers are 3 to 4 cm in diameter and hang one by one at the end of the branch and the end of the stem. The involucre is bell-shaped and yellowish green. The fruit is an achene, cylindrical, about 6mm long, and the crown is 11 to 14mm long, brown and has no feather-like hair. Young shoots are eaten as vegetables, and are distributed in Korea and Japan.

When the natural extract is additionally mixed as an active ingredient of the feed composition of the present invention, the effect of enhancing the growth of animals ingesting the present feed composition can be further improved through the synergistic effect of mixing each active ingredient, and immunity There is also an effect that can maximize the enhancement effect. Furthermore, compared to feeds sold on the market, there is an advantage in providing feeds with improved palatability.

More preferably, the natural extract may be included in 5 to 10 parts by weight of Jindeukchal extract, 5 to 10 parts by weight of Ginseng japonica extract, and 5 to 10 parts by weight of Sanbijang extract, based on 100 parts by weight of salad vegetable by-products.

When included in the above weight range, the synergistic effect of mixing each active ingredient included in the natural extract has the advantage of exhibiting growth enhancement effect and immunity enhancement effect that represent critical significance, while less than the above weight range or the above weight range. If the weight range is exceeded, the effect may be insignificant.

The extract may be extracted using any one selected from the group consisting of water, lower alcohols having 1 to 6 carbon atoms, and mixtures thereof as an extraction solvent.

Specifically, pulverizing the dried leaves; leaching the pulverized product using an organic solvent; drying the sample after leaching; Re-leaching the dried sample using an organic solvent; drying the sample after leaching; leaching with water; And including the step of leaching, it is possible to obtain the Jindeukchal extract.

The step of fractionating the Jindeukchal extract extracted using the organic solvent using an organic solvent may be further included.

A method for preparing the extract may be a conventional extraction method in the art, such as an ultrasonic extraction method, a leaching method, and a reflux extraction method. Specifically, it may be an extract obtained by extracting a natural product from which foreign substances are removed by washing and drying with water, alcohol having 1 to 6 carbon atoms, or a mixed solvent thereof, or may be an extract extracted by sequentially applying the solvents to a sample.

The reflux extraction method is based on 100 mL of alcohol having 1 to 6 carbon atoms, 10 to 30 g of pulverized natural product, 1 to 3 hours of reflux, and 50 to 100% of alcohol having 1 to 6 carbon atoms. More specifically, based on 100 mL of alcohol having 1 to 6 carbon atoms, 10 to 20 g of pulverized natural product, 1 to 2 hours of reflux, and 70 to 90% of alcohol having 1 to 4 carbon atoms.

The leaching method is by 15 to 30 ℃, for 24 to 72 hours and 50 to 100% of alcohol having 1 to 6 carbon atoms. More specifically, at 20 to 25° C. for 30 to 54 hours and 70 to 80% of an alcohol having 1 to 6 carbon atoms.

The ultrasonic extraction method is by 30 to 50 ℃, 0.5 to 2.5 hours and 50 to 100% of alcohol having 1 to 6 carbon atoms. Specifically, at 40 to 50° C. for 1 to 2.5 hours and 70 to 80% of an alcohol having 1 to 6 carbon atoms.

The extraction solvent may be used in an amount of 2 to 50 times, more specifically, 2 to 20 times, based on the weight of the sample. For extraction, the sample may be left for leaching in the extraction solvent for 1 to 72 hours, more specifically 24 to 48 hours.

After extraction, the extract can be fractionated by sequentially applying a fresh fractionation solvent. The fractionation solvent used in the fractionation is at least one selected from the group consisting of water, hexane, butanol, ethylacetic acid, ethyl acetate, methylene chloride, and mixtures thereof, and is preferably ethyl acetate or methylene chloride.

After obtaining the extract or fraction, a method such as concentration or lyophilization may be additionally used.

An eco-friendly animal feed according to another embodiment of the present invention includes the composition.

The feed is necessary for the maintenance or growth of livestock or other animals and fish determined by the Ordinance of the Ministry of Agriculture, Forestry and Fisheries according to the Livestock Act, and includes components such as carbohydrates, proteins, fats, vitamins, minerals, etc. normally necessary for the growth of animals. do. The feed consists of single feed, compound feed and supplementary feed. Single feed is a vegetable, animal or mineral substance that is used directly as feed or used as a raw material for compound feed. Formula feed is a mixture or processing of two or more types of single feed in a certain ratio, and supplementary feed is the quality of the feed. It means adding to the feed to prevent deterioration or to increase the efficacy of the feed. As a condition to be equipped as a good feed for the above feed, the supply capacity of nutrients must be high, it must be harmless and non-toxic to animals, the production must be high and it must be easy to purchase, it must not deteriorate easily, it must be easy to store, and the digestibility of nutrients in the feed must be high. do.

On the other hand, feed can be classified by nutritional value and can be divided into roughage, concentrated feed and supplementary feed.

The roughage is larger than the nutrient content and has a crude fiber content of 18% or more, so the digestible nutrient is small, but it gives a feeling of fullness and improves bowel movement, and straw, hay, and silage belong thereto. The concentrated feed is a feed having a high total amount of digestible nutrients and a low crude fiber content, and is a feed that can have a good effect on the growth of livestock and the production of livestock products. Fodder, etc., belong to this category. In addition, the supplementary feed is also called special feed or scientific feed, which is used to sufficiently supply special nutrients even with a small amount and improve the palatability of the feed and the quality of the product, such as aromatics, flavoring agents, coloring agents, and antioxidants. supplements, mineral feeds (bone meal, limestone, calcium phosphate, table salt, etc.), vitamins, amino acids, etc. Supplementary feed is sometimes used to supplement and supply nutrients that are likely to be lacking in general feed, but also used for the purpose of promoting the growth of young animals, such as antibiotics, hormones, yeast, and unknown growth factors.

The present invention is a feed prepared by using a feed composition containing salad vegetable by-products and lactic acid bacteria as active ingredients, and the effect of the combination of the active ingredients can improve animal growth and improve immunity. Rather, it is a feed that exhibits a better preference than existing feeds sold on the market, and corresponds to a formulated feed.

As non-limiting examples of the feed of the present invention, it means feed for cattle, pigs, horses, chickens, ducks, chicks, dogs, sheep, cats and rabbits.

The feed includes various supplements such as amino acids, inorganic salts, vitamins, antibiotics, antibacterial substances, antioxidants, antifungals, enzymes, live microbial agents, etc. as auxiliary ingredients together with the feed composition of the present invention; cereals such as ground or crushed wheat, oats, barley, corn and rice; vegetable proteins such as rape, soy and sunflower; animal proteins such as blood meal, meat meal, bone meal and fish meal; It may include sugar and dairy products such as various powdered milk and whey powder, but is not limited thereto.

According to the eco-friendly feed composition containing salad vegetable by-products and lactic acid bacteria of the present invention, including natural materials and lactic acid bacteria as active ingredients, there is an effect of improving the growth and immunity enhancement effects of animals ingesting them.

1 is an experimental result confirming the immune enhancing effect of another feed composition according to an embodiment of the present invention through a NO production inhibition experiment.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily implement the present invention. However, the present invention may be embodied in many different forms and is not limited to the embodiments described herein.

manufacturing example

Preparation of salad vegetable by-products

Received celery, red cabbage, mustard leaf, chicory, romaine, lettuce, kale, cucumber, onion, broccoli, rucola and asparagus, which are not commercialized and generated as waste during the manufacturing process of salad for instant food, from a salad manufacturer, and received 1500 to 2000 rpm for 5 to 10 minutes with a grinder, to prepare the vegetables in the form of a mixed powder, and then freeze-drying them to prepare the salad vegetable by-product (SBP) of the present invention.

Production of lactic acid bacteria

Complex lactic acid bacteria for addition to the feed composition of the present invention are Lactobacillus sakei (L1), Leuconostoc mezenteroid subspi. Megenteroides (Leuconostoc mesenteroides subsp. Mesenteroides, L2) and Weissella koreensis (L3) were included, and the strains were obtained by enrichment culture at 37 ° C. for 12 hours in MRS, each of which is a lactic acid bacteria culture medium, It was used in the form of lactic acid bacteria.

Preparation of natural extracts

After obtaining the leaves of Jindeukchal, they were washed, dried, pulverized to prepare a pulverized product, and freeze-dried. 1 mg of 3 g of freeze-dried Jindeukchal was put into a 15 ml tube, and 10 ml of 70% ethanol was added again. Samples were mixed and extracted for 18 hours in a rounded mixing machine. After extraction, only the supernatant was collected after centrifugation at 3000 rpm for 10 minutes. Ethanol (ethanol) contained in the supernatant collected in this way was evaporated at 40 ℃. And the remaining samples were freeze-dried. Jindeukchal extract (SE) used in this experiment was extracted with a yield of 15.5% in Jindeukchal raw material.

On the other hand, using the same method as the preparation method of the Jindeukchal extract (SE), the sagebrush extract (PE) and the Sanbijangi extract (CE) were prepared.

Preparation of feed composition

A feed composition was prepared by mixing the salad vegetable by-products (SBP), lactic acid bacteria (L1 to L3) and natural extracts in the weight range shown in Table 1 below.

Meanwhile, in addition to the ingredients in Table 1 below, 5 parts by weight of sorbitol, 5 parts by weight of sodium phosphate, 5 parts by weight of beta-glucan, 5 parts by weight of protease and 5 parts by weight of citric acid were mixed with respect to 100 parts by weight of salad vegetable by-product (SBP).

T1 T2 T3 T4 T5 T6 T7 T8 T9 T10 SBP 100 100 100 100 100 100 100 100 100 100 L1 15 20 25 30 35 25 25 25 25 25 L2 15 20 25 30 35 25 25 25 25 25 L3 15 20 25 30 35 25 25 25 25 25 SE - - - - - 3 5 7.5 10 12 PE - - - - - 3 5 7.5 10 12 CE - - - - - 3 5 7.5 10 12

(unit: parts by weight)

Experimental example

1. Animal growth improvement experiment

In order to confirm the growth improvement effect of the feed composition of the present invention, an experiment was conducted on 60 weaned piglets. The experiment was conducted by dividing into Comparative Example (Con), in which only commercially available feed was consumed, and Experimental Examples 1 to 10 (M1 to M10), in which feed compositions T1 to T10 of the present invention were included in 10% of the feed, respectively. The experiment period was conducted for a total of 6 weeks, and feed and drinking water were freely supplied.

The experimental results are shown in Table 2 below by measuring the weight (Kg) and average daily weight gain (g / day) of the weaning piglet from 0 to 42 days and taking the average values of each.

weight (kg) Average daily weight gain (g/day) starting weight end weight Con 8.21 29.01 495 M1 8.30 32.54 577 M2 8.11 34.86 636 M3 8.95 35.45 637 M4 8.24 34.98 636 M5 8.44 32.14 564 M6 8.11 32.58 582 M7 8.14 36.88 684 M8 8.17 37.01 686 M9 8.56 36.99 676 M10 8.45 32.01 560

(Unit: kg, g/day)

Referring to Table 2, when the M1 to M10 feed containing the feed composition of the present invention was consumed, it was confirmed that the average daily weight gain by day 42 was higher than that of the comparative example (Con), especially M2 to M10. In the case of M4 and M7 to M9, it can be confirmed that the effect is the most excellent.

Through this, when ingesting the feed composition of the present invention together, it can be confirmed that the growth of animals is improved compared to conventional feed.

2. Immunity enhancing effect test

The immune enhancing effect of the feed composition of the present invention was confirmed by measuring the NO production amount, and the specific experimental method is as follows.

RAW264.7 macrophages were dispensed into a 96 well plate at a concentration of 1.5 Х 10 ⁵ cells/well, and after 18 hours, the plate was treated with 1 mg/mL of T1 to T10 of the above preparation, respectively, and LPS (1 μg/mL ) was added and cultured for 24 hours. 150 μl of cell culture medium and 20 μl of Griess reagent were mixed and reacted in an incubator at 37°C for 10 minutes. A curve was drawn to calculate the NO content.

The results are shown in Figure 1.

Referring to the results of Figure 1, in the case of the feed composition of the present invention, it was confirmed that the amount of NO production was lowered compared to the control group, especially in the case of the T2 to T4 and T7 to T9 compositions, the NO production inhibition effect is more maximized You can check.

Through this, it can be seen that the feed composition in the above range exhibits an excellent immune enhancing effect by effectively suppressing NO production.

3. Evaluation of palatability

For evaluation of the palatability of the feed composition of the present invention, Comparative Example (Con) in which only commercially available feed is consumed and Experimental Examples 1 to 10 ( M1 to M10) were divided into experiments.

Specifically, the palatability test was conducted by simultaneously feeding the same amounts of the comparative and experimental feeds, and 10 weaning pigs were placed for each feed type, feed was supplied at 9:30 every morning, and the consumption rate was measured. The preference was evaluated by expressing the feed consumption rate for 2 weeks as an average value.

The results are shown in Table 3 below.

Con M1 M2 M3 M4 M5 M6 M7 M8 M9 M10 65.78 70.54 79.55 80.11 78.54 72.12 70.45 85.33 86.54 83.55 75.54

(unit: %)

Referring to Table 3, in the case of M1 to M10 containing the feed composition of the present invention, it can be seen that all show a high level of feed consumption compared to Con. In the case of , it was confirmed that the effect showed the best feed consumption rate.

Through this, it can be confirmed that the preference of the feed can be improved when the feed composition of the present invention is included.

Although the preferred embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements of those skilled in the art using the basic concept of the present invention defined in the following claims are also made according to the present invention. falls within the scope of the rights of

Claims (7)

It is an eco-friendly feed composition containing salad vegetable by-products and lactic acid bacteria as active ingredients,
Based on 100 parts by weight of the salad vegetable by-product, further comprising 5 to 10 parts by weight of Jindeukchal extract, 5 to 10 parts by weight of Ginseng japonica extract and 5 to 10 parts by weight of Sanbijang extract
Eco-friendly feed composition. According to claim 1,
The feed composition is excellent in improving animal growth and improving immunity.
Eco-friendly feed composition. According to claim 2,
The animal is selected from the group comprising cows, pigs, horses, chickens, ducks, chicks, dogs, sheep, cats and rabbits
Eco-friendly feed composition. According to claim 1,
The salad vegetable by-product is selected from the group consisting of celery, red cabbage, mustard greens, chicory, romaine, lettuce, kale, cucumber, onion, broccoli, rucola, asparagus and mixtures thereof
Eco-friendly feed composition. According to claim 1,
The lactic acid bacteria include bacteria selected from the group consisting of the genus Lactobacillus, the genus Leuconostoc, the genus Weissella, and a mixture thereof
Eco-friendly feed composition. According to claim 1,
The composition further comprises an additive,
The additive is selected from the group consisting of sorbitol, sodium phosphate, beta-glucan, protease, citric acid, and mixtures thereof.
Eco-friendly feed composition. Comprising the composition according to any one of claims 1 to 6
Eco-friendly animal feed.

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