KR100485057B1 - Animal feed composition comprising an extract of Diospyrous kaki - Google Patents

Abstract

The present invention relates to a feed composition for animals comprising gampi powder, gampi extract containing hydrolysable tannin and hydrolysable tannin, and the like, and specifically, gampi powder, hydrolyzable tannin containing gampi extract and β-glucan (β) The composition of the present invention, which contains additives such as -glucan, vitamin E, L-carnitin and yeast culture, has excellent pigmentation effect of eggshell and egg yolk. (卵) to increase the weight, and also hydrolyzable tannins have antibacterial activity to increase the effect of the branch of the animal (건강한) can raise healthy livestock can contribute to the increase of farm income.

Description

Animal feed composition comprising an extract of Diospyrous kaki

The present invention is an animal comprising an extract such as gampi powder, gampi extract containing hydrolyzable tannin and β-glucan, vitamin E, L-carnitin and yeast culture. It relates to a feed composition for.

Feed is a substance that supplies organic or inorganic nutrients necessary to maintain livestock life and produce milk, meat, eggs and hides. Good feed is rich in nutrients and harmful to livestock. Not only is it high in production, it is always readily available, always fresh and highly digestible, and can be classified into various types according to nutritional value, main ingredient, distribution, moisture content, blending state and processing type.

Feed additives are added to animal feed for the purpose of preventing diseases, supplementing deficiencies, improving feed efficiency and promoting growth, and are used in vitamins, antibiotics, antibacterial agents, antioxidants, antifungal agents, enzymes, probiotics, Amino acids and trace minerals may also be added. In general terms, feed additives are used to enhance the effectiveness of feed by adding or supplementing small amounts to livestock feed, which mainly supplies energy and protein, to maximize livestock production and livestock production. It is distinguished from animal drugs intended for prevention and treatment.

Persimmon is a fruit of Diospyrous kaki THUNB, a fruit tree unique to East Asia, and native to Korea, China, and Japan. There is a record of cultivation of persimmon trees in the oldest agricultural technology book, "Jinmin Writ", and persimmon trees are also included in the Tang Dynasty. There is a record that persimmons were grown in Gyeongsang-do, Goryeong-do, in the `` Anti-medical First-aid Room '' as a fruit grown early in Korea. The main component of persimmon is sugar, which is 15 to 16%, containing a lot of glucose and fructose, and there is a slight difference depending on sweet and astringent persimmons. The astringent taste component is a tannin component called diospyrin, which is easily water-soluble because it is water soluble. The dark spots on the persimmon and rind of the sweet persimmon are tannin-insoluble tannin cells. It is rich in vitamins A and B, and vitamin C contains 30-50mg in 100g. In addition, it contains pectin and carotenoids (Bo Bo-seop and Shin Mingyo, Hyangjedae, Yeonglimsa, pp915-917, 1998).

Tannin is an aromatic compound having a large number of phenolic hydroxyl groups. Although some components are separated into crystalline phases as a single substance, most of the products are mixed with several kinds of substances, Obtained as a white or light brown amorphous powder. There are two types of classification methods, one of which is hydrolysable tannin, which is hydrolyzed by diluting acid and heated to produce gallic acid or erargic acid, and then polymerized red or brown precipitate. It is a method of roughly classifying condensed tannins, which produce phosphorus phlobaphen, and the other is pyrogallol, which mainly produces pyrogallol by alkali melting, and mainly catechol. It is a method of dividing into the catechol system and its intermediate. When hydrolyzed, gallateannin is mainly produced by producing gallic acid, and erratic acid is produced by eraditannin. Many of them are composed of sugars (usually glucose) and esters of one or several phenolcarboxylic acids, and are also hydrolyzed by an alkali or oxygen tanase / takaziastase. Tannins, composed of catechins or catechin derivatives, are widely distributed in the leaves, stems, roots, and berries of plants, especially in persimmons, chestnuts, green tea, and unripe fruits. Among tannins, the astringent taste is water soluble tannin, and the astringent taste is insoluble tannin. Water-soluble tannins are present in many unripe fruits, but as they mature, they become insoluble tannins, in which case the amount of tannins is the same.

Beta-glucan was first discovered in Japan in 1964. It has the property of activating the immune system and is a medium that helps to resist all kinds of antigens such as viruses, bacteria, fungi, parasites and tumors. It has an antioxidant function, which also inhibits the presence of harmful oxygen in the body. In addition, the immune cells, macrophage (microphage) is activated by β-glucan has the effect of protecting the body of the animal from the antigen.

L-carnitin is an essential pseudovitamin that is synthesized in the liver and kidneys. It helps the metabolism of fats and effectively works for energy production. It is responsible for the storage of metabolic energy, migration into cells and concentration of ammonia in the body. Regulates and stimulates the immune system to stabilize cell membranes (Hamuro, J. and G. Chihara, G. Chapter 19).

Accordingly, the present inventors include an extract including gampi powder, gampi extract containing hydrolyzable tannin, and β-glucan, β-glucan, vitamin E, L-carnitin and yeast culture. As a result of raising the composition of the present invention in the feed of chickens, as a result of the excellent egg shell and egg yolk coloring effect and weight of the egg (증가) as well as confirmed the branch effect on the animal was completed the present invention.

An object of the present invention includes an extract such as gampi powder, gampi extract containing hydrolyzable tannin and β-glucan, vitamin E, L-carnitin and yeast culture. It has excellent coloring effect of eggshell and egg yolk and not only increases egg weight, but also hydrolyzable tannins have antimicrobial activity, which makes it possible to raise healthy branches by raising the effect of branching on livestock animals. It is to provide a feed composition.

In order to achieve the above object, the present invention provides a feed composition for animals comprising gampi powder, hydrolysable tannin-containing gampi extract and additives and the like effective in color change of eggshell and egg yolk and weight increase of egg.

In addition, the hydrolyzable tannins have an antimicrobial activity and exhibit an enhanced antimicrobial effect on the animals. The tannins are separated from natural materials such as plants and animals, such as gampi. Or tannins by synthetic methods well known in the art.

The hydrolyzable tannin-containing gampi extract comprises water, a polar solvent soluble extract of a lower alcohol having a carbon number of C ₁ to C ₄ or a mixed solvent thereof or a nonpolar solvent soluble extract such as hexane, ethyl acetate, dichloromethane, and the like. Contains extracts soluble in 70% methanol.

The additives that can be added include one or more additives selected from β-glucan, vitamin E, L-carnitin, yeast culture, and the like.

The gampi powder provides 50 to 80% of the total weight of the composition of the present invention, hydrolyzable tannin-containing gampi extract 1 to 4%, yeast culture contains an animal feed composition characterized in that it contains 10 to 20% .

The animals include mammals such as pigs, cows, goats, fish such as carp and goldfish, and poultry such as pheasants, chickens, ducks, and turkeys.

In addition, the present invention provides an animal feed additive containing the above gampi powder, hydrolyzable tannin-containing gampi extract as a main component.

Method for separating the hydrolyzable tannin-containing gampi extract of the present invention is as follows, the present invention will be described in detail below.

The hydrolyzable tannin-containing gampi extract of the present invention is dried in a cool place for 3 days to 2 weeks, preferably 1 week at room temperature, and then again at 20 to 70 ℃, preferably at a temperature of 40 ℃ or less Natural drying, hot air drying, warm air drying, preferably warm air drying for 3 days to 2 weeks, is dried to a water content of 5 to 20%. The dried gampi is ground to a particle size of 20 to 40 mesh, and then a volume of water, methanol, ethanol and butanol of about 2 to 10 times, preferably about 2 to 5 times, the dry weight of the gampi A lower alcohol such as or a mixed solvent having a mixing ratio of about 1: 0.1 to 1:10, preferably hot water extraction at an extraction temperature of -30 to 30 ° C, preferably -20 to 10 ° C with 70% methanol , Extraction by cold extraction, reflux cooling extraction or ultrasonic extraction, such as one to five times, preferably two to three times to obtain a continuous extraction, then filtered through a filtrate and concentrated under reduced pressure with a rotary vacuum concentrator , The residue can be obtained by vacuum freeze drying, hot air drying or drying by spraying, etc., to obtain crude extracts available in water, lower alcohols or mixed solvents thereof.

In addition, the crude extract of the present invention is suspended in water, and then added to a nonpolar solvent such as ethyl acetate, chloroform or hexane in about 1 to 100 times, preferably about 1 to 5 times, the volume of the suspension. Can be obtained by fractionation with a separatory funnel, preferably 2 to 5 times. It is also possible to further carry out conventional fractionation processes (Harborne JB Phytochemical methods: A guide to modern techniques of plant analysis ., 3rd Ed. Pp 6-7, 1998).

Preferred hydrolyzable tannin-containing gampi extract of the present invention can be obtained by the following process.

For example, a small amount of K ₂ HPO ₄ solution is added to the extracted filtrate, centrifuged at 3000 rpm for 10 to 30 minutes, and the remaining portion is washed with a lower alcohol such as methanol and ethanol and then dissolved in a hydrochloric acid solution. Let's do it. This solution was neutralized with saturated sodium carbonate solution to pH 3.0 to 6.0, preferably pH 3.0 to 4.0, and then dialyzed in dialysis solution for 24 to 48 hours, preferably 30 to 36 hours, and then 0.1 to 10%. , Preferably dissolved in 0.1-5% hydrochloric acid-dimethylformamide (HCL-dimethylformanide) solution, and then injected into a Sephadex LH-20 column to develop 50-70% DMFA solution. It is then dialyzed again with distilled water and lyophilized to obtain a hydrolyzable tannin-containing gampi extract.

The present invention is useful for the color change and egg weight change of eggshell and egg yolk containing the extract of persimmon and persimmon powder obtained by the above method as an active ingredient, as well as an animal feed composition exhibiting an enhanced branch effect on animals. To provide.

The hydrolyzable tannin-containing gampi extract may be 20 to 90% high concentrate, powder or granule form.

The hydrolyzable tannin-containing gampi extract may be organic acids such as citric acid, fumaric acid, adipic acid, lactic acid, malic acid, phosphates such as sodium phosphate, potassium phosphate, acid pyrophosphate, polyphosphate (polyphosphate), polyphenols, catechin (catechin) It may further comprise any one or more of natural antioxidants, such as alpha-tocopherol, rosemary extract, vitamin C, green tea extract, licorice extract, chitosan, phytic acid.

Animal feed composition containing the hydrolyzable tannin-containing extract of the present invention can be added as a supplementary components, such as amino acids, inorganic salts, vitamins, antibiotics, antibacterial substances, antioxidants, antifungal, enzymes, live microbial agents, etc. have.

In addition, the animal feed composition containing the hydrolyzable tannin-containing persimmon extract of the present invention is a grain, for example, crushed or crushed wheat, oats, barley, corn and rice; Vegetable protein feed, such as rape, soybeans and sunflower; Animal protein feeds such as blood meal, meat meal, bone meal and fish meal; Sugars and dairy products, for example, a dry ingredient consisting of various powdered milk and whey powder, and a drying additive, all mixed together with a liquid component, and a component which becomes a liquid after heating, that is, a lipid, for example, an animal liquefied by heating. In addition to the main components such as fats and vegetable fats can be used with substances such as nutritional supplements, digestion and absorption enhancers, growth promoters, disease prevention agents and the like.

The invention is applicable to a number of animal diets, including mammals, poultry and fish. More specifically, the diet is commercially important mammals such as pigs, cows, sheep, goats, laboratory rodents (eg rats, mice, hamsters and gerbils), furry-owned animals (eg mink and foxes). Etc.), and zoo animals (eg monkeys and tailless monkeys), as well as livestock (eg cats and dogs, etc.).

In the method of blending the animal feed composition comprising the composition according to the present invention, the composition of the present invention is incorporated into an animal feed in an amount of about 1 g to 100 g per kg of feed on a dry weight basis.

In addition, the feed mixture is thoroughly mixed and then a viscous granulated or granular material is obtained depending on the degree of grinding of the components. It may be fed as a mesh or formed into a desired discrete shape for further processing and packaging, in which case water is added to the animal feed to prevent separation during storage, followed by conventional pelletization, expansion, Alternatively, it is preferable to go through an extrusion process and excess water may be dried out.

In the above, the animal feed composition containing the hydrolyzable tannin-containing gampi extract according to the present invention has been described, and the present invention will be described in detail with reference to Examples and Experimental Examples.

However, the following Examples and Experimental Examples are only illustrative of the present invention, and the content of the present invention is not limited by the following Examples and Examples.

Example 1 Preparation of Gumpi Powder

After drying moisture-containing gampi (Yeongdong-gun, Chungcheongbuk-do) at room temperature for 1 week at room temperature, warm air for another week at low temperature below 40 ℃ to prevent loss of vitamins A, B and C, etc., and pectin and carotenoids. Drying allowed the water content to be 10% or less. The dried gampi was pulverized using a pulverizer so as to have a particle size of 30 mesh or less to prepare a gampi powder. The gampi powder obtained by the above method was used as the main raw material of the present invention, and its content was made to be 80% or less of the total composition content including the hydrolyzable tannin content.

Example 2. Preparation of Gumpi Extract

A 70% methanol solution corresponding to three times the mass of the dried gampi powder (300 g) obtained in Example 1 was added at -20 ° C, mixed for 20 minutes, and filtered. The procedure was repeated three times, and then extracted. The combined filtrates were combined, and a small amount of 2M K ₂ HPO ₄ solution was added thereto, followed by centrifugation at 3000 rpm for 20 minutes. The remaining part after centrifugation was washed with methanol, dissolved in 1 L of 1.2N hydrochloric acid solution, 1 L saturated sodium carbonate solution was added to neutralize the pH to 4.0, and dialyzed in dialysis solution for 36 hours. The same amount of saturated solution of caffeine was added to the dialysate and centrifuged again at 3000 rpm for 20 minutes. The remaining portion was dissolved in 1% hydrochloric acid-dimethylformamide solution and injected into Sephadex LH-20 column to develop 70% DMFA solution as a developing solvent. After lyophilization, 3 g of gampi extract was obtained.

Example 3. Preparation of β-glucan-containing extract

Sichuan mushrooms were collected and washed with water, and then powdered to a size of 30 mesh using a grinder. 70% methanol solution corresponding to three times the mass of dried situation mushroom powder (300 g) was added at -20 ° C, mixed for 20 minutes, and filtered. The procedure was repeated three times, and then extracted. The combined filtrates were combined, and a small amount of 2M K ₂ HPO ₄ solution was added thereto, followed by centrifugation at 3000 rpm for 20 minutes. The remaining part after centrifugation was washed with methanol, dissolved in 1 L of 1.2N hydrochloric acid solution, 1 L saturated sodium carbonate solution was added to neutralize the pH to 4.0, and dialyzed in dialysis solution for 36 hours. The same amount of saturated solution of caffeine was added to the dialysate and centrifuged again at 3000 rpm for 20 minutes. The remaining portion was dissolved in 1% hydrochloric acid-dimethylformamide solution and injected into Sephadex LH-20 column to develop 70% DMFA solution as a developing solvent. After lyophilization, 30 g of β-glucan-containing extract was obtained.

Example 4 Preparation of Yeast Culture

10 g of BactoYeast extract was dissolved in 1000 ml of distilled water, followed by autoclave. 20 g of agar (Agar) was added to the autoclaved extract to dissolve, and then cultured in a YPD incubator at 37 ° C. After the incubation period was dried in a cool place for one week so that the moisture is less than 10%, and then warm air dried at a low temperature of less than 40 ℃ again. The dried yeast was used to prepare an animal feed composition so that the content per kilogram of 17% or more.

Example 5. Preparation of Animal Feed Composition

Animal feed compositions were prepared by adding vitamin E and L-carnitin (L-carnitin) in addition to the persimmon powder, hydrolyzable tannin-containing extracts, β-glucan-containing extracts, and yeasts obtained in Examples 1 to 4. In the case of hydrolyzable tannins, the content was 1 to 4% or less in consideration of side effects in the livestock body, and the gampi powder contained 50 to 80% including the hydrolyzable tannin content, and the yeast contained 10 to 20% per kg It was made. Small amounts of L-carnitine, β-glucan and vitamin E were added to prepare the animal feed composition of the present invention. The ratio of the animal feed composition of the present invention is illustrated in Table 1.

Composition example W% Composition example W% Gumpi Powder 78 Vitamin E 0.5 Hydrolyzable tannin-containing gampi extract 3.5 L-carnitine 0.5 β-glucan 0.5 Yeast culture 17

Experimental Example 1. Measurement of egg color and egg yolk color change and egg weight change

When the composition of the present invention is added to the feed, the egg laying and egg weight change in egg yolks and egg weights of egg yolks in Boryeong K farm, Chungnam, Korea from August 9 to August 30, 2003 for a total of 21 days 3 When full water was bred for 21 days in a feed containing 0.2% of the composition of the present invention, the color change and weight gain of eggshell and egg yolk were measured in special eggs and eggs.

1-1. Measurement of eggshell color change of special egg and royal egg

Eggshell color change of the egg and egg was measured using an egg shall color fan. When 0.1% of the composition of the present invention was added to the feed, the value of 13.10 and 12.87 in the special eggs and egg yolk at week 1, respectively, was significantly increased by about 2.5%. The experimental results are shown in Table 2 and FIG. 1.

Processing period process Specialty Wang Ran Week 0 11.0 ± 0.33 ^a 11.30 ± 0.37 ^a 1 week 13.10 ± 0.46 ^b 12.87 ± 0.33 ^b 2 weeks 12.73 ± 0.39 ^b 12.57 ± 0.42 ^b 3 weeks 12.60 ± 0.29 ^b 12.40 ± 0.38 ^ab

^{a, b} : mean ± SE ( P <0.05)

1-2. Measurement of egg yolk change of special egg and yellow egg

Egg yolk changes of the egg and the egg were measured using a yolk color fan. When 0.1% of the composition of the present invention was added to the feed, the first week of feeding did not show a significant difference, but showed a tendency to increase, compared to before feeding, and the second week showed a significantly increased value of 8.50. Compared with the egg yolk color change of the special egg and the yellow egg by treatment, the coloring effect of the yellow egg was better. The experimental results are shown in Table 3 and FIG. 2.

Processing period process Specialty Wang Ran Week 0 7.30 ± 0.37 ^a 6.90 ± 0.28 ^a 1 week 7.50 ± 0.20 ^a 8.25 ± 0.14 ^b 2 weeks 8.50 ± 0.20 ^b 8.75 ± 0.16 ^b 3 weeks 8.45 ± 0.20 ^b 8.45 ± 0.21 ^b

^{a, b} : mean ± SE ( P <0.05)

1-3. Measurement of egg weight change between special eggs and eggs

Egg weight change of the egg and egg yolk tended to increase from the first week when 0.1% of the composition of the present invention was added to the feed, and significantly increased from the second week of feeding. In case of yellow egg, the composition of the present invention was added to the feed, and then increased continuously, showing the largest value as 73.23 at the 2nd week of the experiment. The experimental results are shown in Table 4 and FIG. 3.

Processing period process Specialty Wang Ran Week 0 62.68 ± 1.45 ^a 69.69 ± 0.97 ^a 1 week 63.49 ± 0.43 ^a 72.76 ± 0.63 ^b 2 weeks 64.34 ± 0.48 ^b 73.23 ± 0.54 ^b 3 weeks 64.01 ± 0.48 ^b 72.62 ± 0.56 ^b

^{a, b} : mean ± SE ( P <0.05)

Experimental Example 2. Measurement of Branch Effect of Hydrolyzable Tannins

When the feed treated with hydrolyzable tannin of the present invention was fed to pregnant pigs, the effect on the fecal microbial group of sows and piglets after delivery of pregnant pigs In order to investigate the control and the experiment was placed in the experiment as follows.

2-1. Trial feed

Pregnancy pigs (PIC LWY breeder, maternal fertility, average weight 250kg) were placed into four groups of gestational pigs in each treatment group (0.1% tannin of the present invention). And 3.6 kg each (see Table 5).

Pregnancy Pig Feed Fodder forage ingredient(%) ingredient(%) cornflour 60.24 Starch 71.11 wheat 5.00 Fish powder 6.09 Soybean Powder 20.00 replacer 9.77 Fish powder 5.98 TCP 1.21 Replacer 6.02 Salt 0.57 TCP 0.90 Lecithin powder 0.15 Salt 0.02 Soybean oil 3.50 Lysine 0.10 Glucose Luccose 5.00 Soybean oil 0.90 Plasma protein 1.00 Neomycin 0.09 Linsenmycin 0.10 Fenbendazole 0.05 Oralquindox 0.10 Mineral mixtures 0.34 Mineral mixtures 1.07 Vitamin mixtures 0.10 Vitamin mixtures 0.15 Etc 0.35 Etc 0.28 Chemical composition Chemical composition Crude Protein (%) 21.93 Crude Protein (%) 21.36 Ether extract (%) 4.61 Ether extract (%) 7.72 Crude fiber (%) 2.58 Crude fiber (%) 2.32 Ash (%) 5.23 Ash (%) 6.22 NDF (%) 8.65 NDF (%) 6.12 ADF (%) 5.26 ADF (%) 4.19 TDN (%) 89.39 TDN (%) 85.55 ME, Mcal / kg DM 3457.08 ME Mcal / kg DM 3463.24 DE, Mcal / kg DM 3915.24 DE, Mcal / kg DM 4053.12 Lysine 1.38 Lysine 1.32 Methionine + Cysteine 0.81 Methionine + Cysteine 0.80 DCP (%) 18.53 DCP (%) 17.36 NFE (%) 65.65 NFE (%) 62.38 sign(%) 0.75 sign(%) 0.79 calcium(%) 0.81 calcium(%) 0.95 potassium(%) 0.89 potassium(%) 0.86 salt(%) 0.20 salt(%) 0.51 Vitamin A, IU 9.22 Vitamin A, IU 13.47 Vitamin D, IU 0.92 Vitamin D, IU 1.40 Vitamin E, IU 49.56 Vitamin E, IU 56.14 Vitamin K, IU 4.03 Vitamin K, IU 7.47

In Table 5, TCP is tricalcium phosphate, NDF is neutral detergent fiber, ADF is acid detergent fiber, TDN is total digestible nutrients, and ME is metabolizable energy. energy, DE means digestive energy, DCP means dicalcium phosphate and NFE means soluble nitrogen.

2-2. Experimental Effect of Hydrolyzable Tannins

Experimental feed was given from 15 days before delivery in cages that were made to feed individual animals, and we reared them in weaning time (21 days after delivery) in order to increase the weight of piglets and observe diseases. Feeds for each treatment group were fed twice a day, and in order to measure the actual feed intake during the adaptation period, the actual feed intake was calculated by measuring the remaining feed in the following day at the morning feed (07:00). The pay amount was adjusted so that there was no remaining amount. The experiment period was preliminary adaptation period of 15 days before delivery, and a total of 36 days of experiments were performed with 21 days after delivery. For fecal microbiological measurements, feces of sows and piglets were collected from weaning to measure the water content of feces and the distribution of pathogenic microorganisms.

General components of the test feed were analyzed for dry matter, crude protein and crude fat according to the well-known AOAC method (1990), and microbial analysis in minutes was carried out in the total viable count, E coli and Salmonella. In order to measure colonies of such pathogenic microorganisms, the number of colonies was measured after culturing for 24 hours using nutrient agar.

As a result of the above experiment, as shown in Table 6 below, in the group which did not administer the tannin component of the present invention to piglets and sows, the average of piglets was 3.25 ± 10 ² (CFU / ml) and sow meal showed an average of 6.75 ± 10 ² (CFU / ml) of E. coli, but when the tannin component of the present invention was administered to piglets and all flour, the average was 2.0 for piglets. ± 10 ² (CFU / ml), sow powder average 3.0 ± 10 ² (CFU / ml) was confirmed that the antimicrobial activity of the tannin component of the present invention.

Sample name moisture(%) Viable Count (CFU / ml) Escherichia coli (CFU / ml) Salmonella Piglets +1 77.57 8.0 ± 10 ⁷ 2.0 ± 10 ² ND Piglets +2 77.73 9.0 ± 10 ⁷ 3.0 ± 10 ² ND Piglets +3 75.17 7.0 ± 10 ⁷ 2.0 ± 10 ² ND Piglets +4 76.55 9.0 ± 10 ⁷ 1.0 ± 10 ² ND Piglets -1 70.00 4.0 ± 10 ⁷ 1.0 ± 10 ² ND Piglets -2 69.14 5.0 ± 10 ⁷ 4.0 ± 10 ² ND Piglets -3 72.66 1.0 ± 10 ⁷ 1.0 ± 10 ² ND Piglets -4 76.35 1.0 ± 10 ⁷ 7.0 ± 10 ² ND Sow meal +1 72.00 5.0 ± 10 ⁷ 3.0 ± 10 ² ND Sow meal +2 71.66 4.0 ± 10 ⁷ 2.0 ± 10 ² ND Sow meal +3 73.50 3.0 ± 10 ⁷ 5.0 ± 10 ² ND Sows +4 70.05 11.0 ± 10 ⁷ 2.0 ± 10 ² ND Sow flour -5 68.93 9.0 ± 10 ⁷ 8.0 ± 10 ² ND Sow meal -6 72.26 11.0 ± 10 ⁷ 7.0 ± 10 ² ND Sows -7 72.12 10.0 ± 10 ⁷ 5.0 ± 10 ² ND Sows -8 73.17 8.0 ± 10 ⁷ 7.0 ± 10 ² ND

In the sample name, + means that the tannin component of the present invention is administered,-means that the tannin component is not administered, and ND (Not Detection) means that no bacteria are present.

2-3. Comparison of Antimicrobial Activity of Tannin Components Extracted from Tannin Components and Acorns, Persimmons and Green Teas of the Present Invention

In order to confirm the antimicrobial activity of the tannin component of the present invention, an experiment comparing the antimicrobial activity with the tannin component extracted from acorns, sol and green tea was performed using a medium diffusion method. Antibacterial activity of each tannin component by treating E. coli (Eshericaia coli, ATT 25922) and Salmonella (Salmonella) at 24 hours under optimal viable cell temperature and then treating the tannin component of the present invention and the tannin component extracted from acorns, sol and green tea Was confirmed by observing the diameter of the growth inhibition ring of the bacteria.

As a result of the above experiment, as shown in Table 7 below, in the case of E. coli, except for the tannin component extracted from green tea, the bacterial growth inhibition was observed, and in the case of Salmonella, the hydrolyzable tannin component of the present invention was extracted from another substance. It was confirmed that growth inhibition phenomenon is remarkable compared to the components.

Tannins of the invention brush Acorn green tea Escherichia coli + + + - Salmonella +++ ++ ++ +

The antimicrobial activity was measured as the diameter (φ) of the growth inhibitory ring, where ++ is φ> 20 mm, + is φ = 10-20 mm, and-is no growth inhibition ring.

The present invention relates to a feed composition for animals containing persimmon extract, the composition of the present invention can produce high-quality eggs by sharpening eggshell color and egg yellow color and increasing the weight of eggs, and also hydrolyzable tannins have antibacterial activity. It can help to increase farm income by increasing the governor effect of animals so that they can raise healthy livestock.

 1 is a diagram showing the improvement effect of eggshell color when 0.1% of the composition of the present invention in the feed,

Figure 2 is a view showing the improvement effect of yolk yellow when 0.1% of the composition of the present invention in the feed,

Figure 3 is a diagram showing the effect of improving the weight of the egg when 0.1% of the composition of the present invention in the feed,

Figure 4 is a photograph showing the antimicrobial activity of hydrolyzable tannin against bacterial growth by the medium diffusion method (Ager Diffusion method), the hydrolyzable tannin and acorn of the present invention for E. coli (Fig. 4a) and Salmonella (Fig. 4b) The photograph shows the growth inhibition of the tannin bacteria extracted from, persimmon and green tea.

Claims (7)

Animal containing 50-80% of persimmon powder, 1-4% of hydrolyzable tannin-containing persimmon extract, and 10-20% of yeast culture and effective for egg color and egg yolk color change and egg weight increase Feed composition for. The animal feed composition according to claim 1, wherein the antimicrobial activity of the hydrolyzable tannins results in an enhanced antimicrobial effect on the animal. delete delete delete delete Animal feed additives containing 50 to 80% of the skin extract of claim 1, 1 to 4% of the skin extract containing hydrolsable tannin.