KR102026777B1 - Method for preparing fermented soybean meal for replacing fishmeal using Anti-Nutritional Factor controlling strain Microbacterium hydrothemale KBCBS1021 - Google Patents

Abstract

The present invention relates to an anti-nutrient-factor controlling microorganism M. hydrothemale KBCBS1021 and a method for preparing fermented soybean meal for fish meal replacement using the same, and to fermented soybean meal and fermented soybean meal using M. hydrothemale KBCBS1021 strain or extracts or fractions derived from the strain according to the present invention. The fermentation composition as an active ingredient exhibits a reducing effect on anti-nutritional factors compared to before fermentation, and thus may be usefully used as a nutritional source for various compositions including feed.

Description

Method for preparing fermented soybean meal for replacing fishmeal using Anti-Nutritional Factor controlling strain Microbacterium hydrothemale KBCBS1021}

The present invention relates to a control microorganism hangyoung yanginja micro tumefaciens dihydro deomeol (Microbacterium hydrothemale) KBCBS1021 fermented soybean meal and fish meal Alternative production method for using the same.

Feeding is a large part of production costs in breeding and aquaculture and has a significant impact on production growth. However, since water pollution from aquaculture is related to the feed being fed, the development of formulated feed should be considered first in terms of aquaculture productivity and environmental protection. To this end, we are investing a lot in the development of environmentally friendly, low pollution and high efficiency feed for the sustainable supply of safe and healthy feeds around the world.

Currently, fishmeal, the most common protein source for feed, is an animal protein and contains abundant protein content, which is an essential ingredient for growth. It also contains various kinds of amino acids and has an anti-nutiritonal factor. Due to its low digestibility, it is said to be the most ideal feed ingredient for both domestic and farmed fish. However, Peru and Chile, the major fishmeal exporters, produced 2.68 million tonnes in 2008, but 1.161,000 tonnes in 2012 and 855,000 tonnes in 2013, indicating a decrease in fishmeal production. Due to the occurrence of warm seawater on the coast of South America, which hindered the formation of enchovy fish group, a fishmeal material, and reduced production of major fishmeal exporters such as Peru. There is a growing interest in.

The French National Institute for Agriculture Research has launched a research project called Perspectives of Plant Protein Use in Aquaculture (PEPPA) under the EU's fifth research plan. Research is also underway to reduce fishmeal consumption in aquaculture feeds.

Examples of fish meal replacement proteins include soybean meal, soy protein isolate, canola meal, corn gluten meal and other vegetable substances. There is a growing tendency to use materials. These substances are available in just one quarter of the fishmeal price in 2011, which is sufficient to supply them, and because they are plant proteins, they are known to be free from animal protein-borne diseases such as scrapie.

Soybean meal refers to the residue left from squeezing soybean oil, which is 55-56% protein, 13-14% soluble carbohydrates, 21-22% soluble carbohydrates, 1% fat and 4-6% minerals, based on dry conditions. It contains about. The advantages of plant materials such as soybean meal are the low price and high protein content. However, the disadvantages of using these vegetable proteins instead of fishmeal include lower levels of omega-3 fatty acids than animal proteins, and amino acids such as threonine and tyrosine in soybean meal. As a disadvantage, vegetable protein has some deficiencies in the composition of essential amino acids required for livestock, and there is a problem of nutritional imbalance in which vitamin, minerals and UGF (Unknown Growth Factor) contents are not excellent.

One problem with such an imbalance of nutrient content is the presence of anti-nutrients in plants. For example, trypsin inhibitor, an anti-nutritional factor (ANF) contained in soybeans, interferes with the proper enzymatic function of trypsin and chymotrypsin, leading to Lowers usability. In addition, anti-nutrient factors include hemagglutinin, a hemagglutinin, phytate interacting with proteins and minerals, and raffinose and stachyose, which cause diarrhea and abdominal pain in livestock. Oligosaccharides and polysaccharides that inhibit absorption of nutrients are known. In particular, since these anti-nutritive factors affect young livestock more, they limit the amount of soybean meal added to young livestock feed. In farmed fish, anti-nutritional factors may cause problems that take a long time to mature into adults. Due to these problems, in general, animal protein such as soybean meal is not widely used as a protein source, and in particular, it is known that soybean meal components cannot be used in fish feed.

Therefore, in order to use vegetable protein as a substitute for fish meal, reducing the anti-nutritive factors present in the vegetable protein source remains a challenge. Currently, a method of reducing the amount of trypsin inhibitor by heating to 39 mg / g or less before heating is also used. However, the destruction of other nutrients and the remaining antimicrobial activity in small individuals such as the offspring of breeding animals There are problems such as the effects of the two factors, and some anti-nutritional factors, such as α-galactoside and α-galactoside and galactomannan, have a high resistance to heat treatment and are limited.

Korean Patent Publication No. 10-2011-0027535 discloses a method for reducing anti-nutritional factors by adding Bacillus bacteria to soybean meal and fermenting. Korean Patent No. 10-0645284 also discloses Bacillus subtilis GR-101 and Aspergillus. Methods for reducing anti-nutritional factors through the addition of microorganisms have been developed, including the introduction of a solid fermented soy protein processing method that enhances the enzyme production performance using Suri materials GB-107.

The present inventors screened a novel microorganism that suppresses anti-nutritive factors, and completed the present invention by confirming that anti-nutritive factors were reduced in fermented soybean meal prepared using the same.

Republic of Korea Patent Publication No. 10-2011-0027535 Republic of Korea Patent No. 10-0645284

An object of the present invention to provide a control microorganism hangyoung yanginja micro tumefaciens dihydro deomeol (Microbacterium hydrothemale) KBCBS1021 strains fermented soybean meal and fish meal Alternative production method for using the same.

In order to achieve the above object, the present invention provides a microorganism hangyoung yanginja control micro tumefaciens dihydro deomeol (Microbacterium hydrothemale) KBCBS1021 strain deposited as accession No. KCTC18516P.

The present invention also provides a composition for inhibiting anti-nutritional factor (ANF) activity in soybean meal containing M. hydrothemale KBCBS1021 strain deposited with Accession No. KCTC18516P or a culture thereof as an active ingredient.

The present invention also provides a method of preparing a fermented soybean meal comprising the following steps:

1) preparing a culture by culturing the M. hydrothemale KBCBS1021 strain deposited with accession number KCTC18516P;

2) mixing the culture of step 1) with stripped soybean meal to prepare a mixture; And

3) fermenting the mixture of step 2).

In addition, the present invention provides a feed composition containing the fermented soybean meal prepared by the above method as an active ingredient.

Microbacterium hydrothemale KBCBS1021 strain or fermented soybean meal using the culture thereof according to the present invention exhibits an effect of reducing anti-nutritive factors compared to before fermentation, and thus can be usefully used as a nutritional source for various compositions including feed. have.

1 is a microbacterium hydrodermal ( Microbacterium) hydrothemale ) API ZYM kit analysis of KBCBS1021 strain.
2 is a strain of M. hydrothemale KBCBS1021 purely cultured on nutrient agar medium.
Figure 3 is a schematic diagram showing the molecular relationship of the 16S rRNA sequence of M. hydrothemale KBCBS1021 strain.

Hereinafter, the present invention will be described in detail.

The present invention provides a micro tumefaciens dihydro deomeol (Microbacterium hydrothemale) KBCBS1021 strain deposited as accession No. KCTC18516P.

The strain comprises the 16S rRNA nucleotide sequence set forth in SEQ ID NO: 1.

In a specific embodiment of the present invention, the strains inhabiting the sand, seawater and seaweeds of the region are separated from the main beaches in order to isolate the basophilic microorganisms that can be applied to the screening and fish farming environment having an anti-nutrient factor reduction effect. It was. In the selected strains, strains excellent in producing galactosidase, which are known to be involved in controlling anti-nutritional factors, were selected (FIGS. 1 and 2 and Table 1). After confirming that the selected strain is a novel strain by 16S rRNA sequencing (FIG. 3), it was named ' Microbacterium hydrothemale KBCBS1021' and deposited in a biological resource center in the Korea Research Institute of Bioscience and Biotechnology.

In addition, the present invention is deposited with M. Accession No. KCTC18516P . It provides a composition for inhibiting the anti-nutritional factor (ANF) activity in soybean meal containing the hydrothemale KBCBS1021 strain or a culture thereof as an active ingredient.

The anti-nutritive factor is any one or more selected from the group consisting of trypsin inhibitor (TI), raffinose, stachiose, lectin, urease, lipoxygenase, glucocyanide and anti-vitamin factor, And may specifically be a trypsin inhibitor, raffinose or stachiose.
The lectin may be phytohemagglutin.

The strain or culture may be to direct culture of the strain, or to use an extract or fraction derived from the strain, but is not limited thereto.

The direct culture may be a culture medium in which the strain is cultured or filtered by a physical method.

The composition can be easily administered directly in animal feed or in combination with other ingredients by oral formulation, injection or transdermal, alone or separately from the feed. Dosage varies in range depending on the animal's weight, health condition, diet, method of administration and severity of disease. As is well known in the art, the daily dosage is about 0.1-10 mg / g, specifically 0.05-1 mg / g, and more preferably, once or several times a day.

In a specific embodiment of the present invention, after the fermentation of a culture medium of M. hydrothemale KBCBS1021, a novel strain having an anti-nutritive effect reduction effect, with soybean meal, the harvested fermented soybean meal has a significantly lower anti-nutritive content compared to before fermentation. It was confirmed to lose (Table 5). Through this, the composition for inhibiting the anti-nutritional factor (ANF) activity in soybean meal according to the present invention was found to be useful for reducing the anti-nutritional factor.

The present invention also provides a method of preparing a fermented soybean meal comprising the following steps:

1) M deposited with accession number KCTC18516P . preparing a culture by culturing the hydrothemale KBCBS1021 strain;

2) mixing the culture of step 1) with stripped soybean meal to prepare a mixture; And

 3) fermenting the mixture of step 2).

The culturing of step 1) may be performed using a medium containing the following components, but is not limited thereto: a) from the group consisting of arabinose, fructose, fructose, sucrose and glucose. 0.1 to 50 parts by weight, specifically 0.5 to 20 parts by weight, more specifically 1 to 10 parts by weight of any one or more carbon sources selected; b) 0.01 to 50 parts by weight of at least one nitrogen source selected from the group consisting of arginine, tryptone, beef extract, soy bean meal, yeast extract Specifically, 0.05 to 20 parts by weight, more specifically 0.1 to 5 parts by weight; And c) FeCl ₂ · 7H ₂ O, AgNO ₃ , MgSO ₄ · 7H ₂ O, K ₂ HPO ₄ , CaCl ₂ · 2H ₂ O, FeSO ₄ · 7H ₂ O or MnSO ₄ · 4-5H ₂ O At least one inorganic salt selected from 0.001 to 5 parts by weight, specifically 0.005 to 1 part by weight, more specifically 0.01 to 0.5 part by weight.

Mixing of the step 2) is 1 to 80 parts by weight of the culture, specifically 3 to 50 parts by weight, more specifically 5 to 30 parts by weight, 20 to 99 parts by weight of peeled soybean meal with respect to 100 parts by weight of the mixture Specifically, the mixture may be 50 to 97 parts by weight, more specifically 70 to 95 parts by weight.

Fermentation of step 3) may be performed for 3 hours to 15 days, specifically 1 to 7 days, more specifically 2 to 5 days.

Step 3) may be added after the drying step, 4) may be 3 to 15 days after fermentation, specifically 1 to 7 days, more specifically 2 to 5 days to dry.

In a specific embodiment of the present invention a) 1 to 10 parts by weight of any one or more carbon sources selected from the group consisting of arabinose, fructose, fructose, sucrose and glucose; b) 0.1 to 5 parts by weight of at least one nitrogen source selected from the group consisting of arginine, tryptone, beef extract, soy bean meal, yeast extract ; And c) FeCl ₂ · 7H ₂ O, AgNO ₃ , MgSO ₄ · 7H ₂ O, K ₂ HPO ₄ , CaCl ₂ · 2H ₂ O, FeSO ₄ · 7H ₂ O, MnSO ₄ · 4-5H ₂ O As a result of inoculating M. hydrothemale KBCBS1021 strain in a medium containing 0.01 to 0.5 parts by weight of any one or more inorganic salts selected from the above, it was confirmed that bacteria were cultured at a higher density than general LB medium (Table 4). After fermenting the culture medium of M. hydrothemale KBCBS1021 with soybean meal, the fermented soybean meal was found to have a significantly lower anti-nutritive content compared to before fermentation (Table 5).

In addition, the present invention 1) M deposited with accession number KCTC18516P . preparing a culture by culturing the hydrothemale KBCBS1021 strain; 2) mixing the culture of step 1) with stripped soybean meal to prepare a mixture; And 3) provides a feed composition containing a fermented soybean meal prepared by the method comprising the step of fermenting the mixture of step 2) as an active ingredient.

The composition may further include materials that can be added to the feed, such as cereals, algae, other vegetable oils, animal feed raw materials, other feed raw materials, tablets, and the like.

The grains may include, for example, mairo, wheat, barley, oats, rye, brown rice, buckwheat, crude, sorghum, blood, corn, soybean, and the like.

The algae may include, for example, rice bran, skim rice bran, bran, powder, wheat, embryo, barley bran, screening, pellets, corn bran, corn germ, and the like.

The other vegetable oils include oils other than soybean meal, such as soybean meal, flax seed, cottonseed meal, peanut gourd, safflower gourd, palm gourd, palm gourd, houma gourd, sunflower gourd, rapeseed gourd, Kapok meal, Mustard and the like.

The animal feed material may include, for example, additional fish meal, meat meal, fish bone meal, blood meal, bone meal, animal by-products, feather meal, silkworm, skim milk powder, casein, dry whey, and the like.

The other feed ingredients include plant foliage (alfalfa, haycube, alfalfa leaf powder, pseudo acacia powder, etc.), corn processing industrial by-products (corn gluten, wheat, corn gluten feed, corn staple, etc.), starch processed products, sugar, fermentation. Industrial products (yeast, beer foil, malt sprouts, alcohol foil, walnuts, etc.), agricultural production by-products (citrus fruits, tofu, coffee, cocoa, etc.), others (cassava, bean, guam, seaweed, Krill, spirulina, chlorella, minerals, etc.) etc. are mentioned.

Examples of the purified products include proteins (casein, albumin, etc.), amino acids, sugars (starch, cellulose, sucrose, glucose, etc.), minerals, vitamins, other nutritional supplements, growth promoters, digestive absorption accelerators, disease prevention agents, and the like.

The content of fermented soybean meal in the feed can be appropriately adjusted according to the type of animal, age, application form, desired effect, etc., for example, 1 to 99 parts by weight, 10 to 90 parts by weight, or 20 to 80 parts by weight. However, it is not limited thereto.

The composition may be used in animal feed for mammals, birds, reptiles, amphibians, fish and the like, but is not limited thereto. Preferably, the composition may be used for farmed fish feed.

The type of livestock to which the feed is applicable is not particularly limited. For example, the feed of the present invention is for pet food such as dogs, cats and mice, for livestock feed such as cattle and pigs, and for poultry such as chicken and turkey. It can be used as a feed for fish such as feed, sea bream, defense, catfish, crucian carp, flounder, eel and the like.

In a specific embodiment of the present invention a) 1 to 10 parts by weight of any one or more carbon sources selected from the group consisting of arabinose, fructose, fructose, sucrose and glucose; b) 0.1 to 5 parts by weight of at least one nitrogen source selected from the group consisting of arginine, tryptone, beef extract, soy bean meal, yeast extract ; And c) FeCl ₂ · 7H ₂ O, AgNO ₃ , MgSO ₄ · 7H ₂ O, K ₂ HPO ₄ , CaCl ₂ · 2H ₂ O, FeSO ₄ · 7H ₂ O, MnSO ₄ · 4-5H ₂ O As a result of inoculating M. hydrothemale KBCBS1021 strain in a medium containing 0.01 to 0.5 parts by weight of any one or more inorganic salts selected from the above, it was confirmed that bacteria were cultured at a higher density than general LB medium (Table 4). After fermenting the culture medium of M. hydrothemale KBCBS1021 with soybean meal, the harvested fermented soybean meal has a significantly lower content of trypsin inhibitor (TI), raffinose and starchiose, which are anti-nutritive factors, as compared to before fermentation. It was confirmed that (Table 5), it can be usefully used for feed.

Hereinafter, the present invention will be described in detail by way of examples.

However, the following Examples and Experimental Examples are only for illustrating the present invention, the present invention is not limited by the following Examples and Experimental Examples.

Example  1. Main Anti-nutritional factors  Selection of Controlled Microorganisms

Sand, seawater, and seaweeds were sampled from major beaches in Gyeongnam (Sacheon, Namhae, Tongyeong, etc.) to isolate basophils that could be applied to fish farming environments. Each sampled sample was diluted 10-fold in the prepared sterile water, stirred for 30 minutes, and then diluted in sterile water, and plated in 5% NaCl LB agar (NaCl 5%, LB broth 2.5%, Bacto agar 2%) medium. . The plated medium was incubated for 24 hours in a 30 ° C incubator, and 111 kinds of basophilic strains having a density of 1.0 × 10 ³ cfu / g (ml) or more among the strains grown in the medium were selected.

In order to screen the galactosidase-producing strains known to be involved in the control of Raffinose and Stachyose in 111 selected basophils, API ZYM kit (Biomerieux, USA) was used. Selected strains were inoculated in LB broth after pure separation, shaken for 48 hours at 30 ° C, 120rpm, and then centrifuged at 6,000rpm for 15 minutes using a refrigerated high-speed centrifuge (Hanil Science, Korea). 300 μl of the culture was sampled and placed in 2 ml of suspension medium to adjust the turbidity of McFarland standards 4 to 5. At the same time, the strips were placed in the prepared trays, and the prepared culture suspension was inoculated with 100 µl each. After incubating for 4 hours in an incubator at 35 ° C, ZYM A and ZYM B reagents were added sequentially and reacted for 5 minutes.

As a result, as shown in Figure 1 and Table 1, the final selection of KBCBS1021 strain having an expression index of alpha-galactosidase and beta-galactosidase 3 or more.

Strain 9. Trypsin 10.Alpha-chymotrypsin 13.alpha-galactosidase 14.beta-galactosidase KBCBS1021 One 0 3 4

Example  2. Identification of Strains Based on Genetic Characteristics

KBCBS1021 strain selected in Example 1 was inoculated in nutrient agar medium (Nutrient agar, Difco, USA) as shown in Figure 2 and incubated for 3 days at 28 ℃ was confirmed to be a single strain. After inoculating the strain into nutrient broth, the culture was shaken at 28 ° C. for 2 days, and the genomic DNA of the strain was extracted by modifying the benzyl chloride method. 16S rRNA gene of the strain in the extracted genomic DNA was subjected to PCR (Polymerase Chain Reaction) in the same manner as Table 2, and the primers used are shown in Table 3.

Temperature time number of cycles 95 ℃ 2 min 1 cycle 94 ℃ 30 sec 30 cycles 58 ℃ 30 sec 72 ℃ 45 sec 72 ℃ 5 min 1 cycle

SEQ ID NO: primer order 2 Primer 27F 5'-AGAGTTTGATCCTGGCTCAG-3 ' 3 Primer 1492R 5'-GGTTACCTTGTTACGACT T-3 '

The obtained 16S rRNA PCR product was stored at 4 ° C, and the PCR amplification product was purified using PCR Product Purification Kit (Qiagen), and the purified product was analyzed by nucleotide sequence using Genetic analyzer 3730xl (Applied biosystems). The 16S rRNA gene infectious sequence (1,349bp) represented by 1 was determined.

Homology search was performed in the database of DDBJ / NCBI / Genebank for 16S rRNA sequences of the strain. Molecular phylogenetic analysis based on the 16S rRNA gene sequence revealed a species belonging to the genus Microbacterium sp. In particular, the selected strain was found to exhibit a 99% flexible relationship with Microbacterium hydrothemale 0704C9-2 ^T (HM222660), and the selected strain was referred to as' Microbacterium hydrothemale It was named KBCBS1021 ', and it was deposited on 22 November 2016 at the Biological Resource Center of Korea Research Institute of Bioscience and Biotechnology (Accession Number: KCTC18516P).

Example  3. Preparation of Mass Culture and Mass Culture of Strains

The present inventors put 1 ton of water into the mass incubator for mass cultivation of the strain selected in Example 2, 2% glucose as carbon source, 0.5% yeast extract as nitrogen source, MgSO ₄ · 7H ₂ O as inorganic salt. After preparing a medium containing 0.02% and K ₂ HPO ₄ 0.1%, autoclaved at 121 ℃ for 15 minutes, inoculated M. hydrothemale KBCBS1021 bacteria and incubated for 48 hours at 100 rpm at 30 ℃, and then nutrient agar medium The density in the culture was measured by the step dilution smear method.

After preparing the mass culture medium as described above, inoculated with M. hydrothemale KBCBS1021 strain and incubated for 48 hours, it is shown in Table 4 compared with the result of culturing in the general LB broth medium.

Strain name LB ( Luria - bertani ) broth Mass media ( TM ) M. hydrothemale KBCBS1021 5.3 × 10 ⁶ cfu / ml 1.6 × 10 ⁹ cfu / ml

As shown in Table 4, the mass culture medium showed a higher density than the density cultured in LB broth.

Example  4. Anti-nutritional factors  Controlled Fermented Soybean Meal of  Produce

The inventors of the present invention, using the large-volume broth prepared in Example 3 to control the anti-nutritional factors contained in the soybean soybean meal 800Kg of molten soybean meal, 2% glucose as a carbon source, 0.5% yeast extract (Yeast extract) as a nitrogen source, inorganic salts 0.02% MgSO ₄ 7H ₂ O and 0.1% K ₂ HPO ₄ were added, and 150 l of the microbacterium hydrodermal 1021 ( M. hydrothemale KBCBS1021) bulk culture medium according to Example 3 was added to a 1 ton fermenter and mixed. It was fermented for 3 days with mixing for 10 minutes every hour. After fermentation, the mixture was mixed for 45 minutes every hour, dried for 3 days, and the fermented soybean meal with controlled anti-nutritional factors was collected. In order to verify the control ability of the anti-nutritional factors, the treated soybean meal (non-treated) was used as a control before mixing the culture medium, and the samples were sampled after the fermentation of soybean meal. 5 is shown.

division Stripped Soybean Meal M. hydrothemale
KBCBS1021 Fermented Soybean Meal Antitrypsin factor (mg / g) 3.27 0.34 Raffinose (%) 0.27 0.02 Starchios (%) 0.50 0.10

As shown in Table 5, the anti-nutritional factor content of soybean meal fermented using M. hydrothemale KBCBS1021 strain is significantly lower than before fermentation, according to the present invention. Soybean meal fermentation was found to have an anti-nutritive effect.

Korea Research Institute of Bioscience and Biotechnology KCTC18516P 20161122

<110> Korea Bio Chemical Co, Ltd. <120> Method for preparing fermented soybean meal for replacing          fishmeal using Anti-Nutritional Factor controlling strain          Microbacterium hydrothemale KBCBS1021 <130> 2017P-08-033 <160> 1 <170> KoPatentIn 3.0 <210> 1 <211> 1349 <212> RNA <213> Unknown <220> <223> Microbacterium hydrothemale KBCB1021 <400> 1 gcttgcttgg tggatcagtg gcgaacgggt gagtaacacg tgagcaacct gccctggact 60 ctgggataag cgctggaaac ggcgtctaat actggatatg agctctcatc gcatggtggg 120 ggttggaaag attttttggt ctgggatggg ctcgcggcct atcagcttgt tggtgaggta 180 atggctcacc aaggcgtcga cgggtagccg gcctgagagg gtgaccggcc acactgggac 240 tgagacacgg cccagactcc tacgggaggc agcagtgggg aatattgcac aatgggcgaa 300 agcctgatgc agcaacgccg cgtgagggat gacggccttc gggttgtaaa cctcttttag 360 cagggaagaa gcgaaagtga cggtacctgc agaaaaagcg ccggctaact acgtgccagc 420 agccgcggta atacgtaggg cgcaagcgtt atccggaatt attgggcgta aagagctcgt 480 aggcggtttg tcgcgtctgc tgtgaaatcc cgaggctcaa cctcgggcct gcagtgggta 540 cgggcagact agagtgcggt aggggagatt ggaattcctg gtgtagcggt ggaatgcgca 600 gatatcagga ggaacaccga tggcgaaggc agatctctgg gccgtaactg acgctgagga 660 gcgaaagggt ggggagcaaa caggcttaga taccctggta gtccaccccg taaacgttgg 720 gaactagttg tggggaccat tccacggttt ccgtgacgca gctaacgcat taagttcccc 780 gcctggggag tacggccgca aggctaaaac tcaaaggaat tgacggggac ccgcacaagc 840 ggcggagcat gcggattaat tcgatgcaac gcgaagaacc ttaccaaggc ttgacataca 900 ccagaacggg ccagaaatgg tcaactcttt ggacactggt gaacaggtgg tgcatggttg 960 tcgtcagctc gtgtcgtgag atgttgggtt aagtcccgca acgagcgcaa ccctcgttct 1020 atgttgccag cacgtaatgg tgggaactca tgggatactg ccggggtcaa ctcggaggaa 1080 ggtggggatg acgtcaaatc atcatgcccc ttatgtcttg ggcttcacgc atgctacaat 1140 ggccggtaca aagggctgca ataccgtgag gtggagcgaa tcccaaaaag ccggtcccag 1200 ttcggattga ggtctgcaac tcgacctcat gaagtcggag tcgctagtaa tcgcagatca 1260 gcaacgctgc ggtgaatacg ttcccgggtc ttgtacacac cgcccgtcaa gtcatgaaag 1320 tcggtaacac ctgaagccgg tggcccaac 1349

Claims (5)

Microbacterium hydrothemale KBCBS1021 strain deposited with accession number KCTC18516P.
A composition for inhibiting the anti-nutritional factor (ANF) activity in soybean meal containing the strain of claim 1 or a culture thereof as an active ingredient.
The method of claim 2, wherein the anti-nutritive factor is selected from the group consisting of trypsin inhibitor (TI), raffinose, stachiose, lectin, urease, lipoxygenase, glucocyanide cyanide and anti-vitamin factor Any one or more.
Method for producing a fermented soybean meal comprising the following steps:
1) culturing the strain of claim 1 to prepare a culture;
2) mixing the culture of step 1) with stripped soybean meal to prepare a mixture; And
3) fermenting the mixture of step 2).
Feed composition containing a fermented soybean meal prepared by the method of claim 4 as an active ingredient.

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