KR20010084854A - Functional food containing extract of Platycodon grandiflorusjacqin A De Candolle and process and for preparation thereof - Google Patents

Abstract

PURPOSE: A method for producing a functional food made from the extract of Platycodon grandiflorum with various kinds of herbs and food additives is provided. This healthful food can promote immune activity of human body and so treat bronchial diseases. CONSTITUTION: The method includes the following steps of: (i) mixing Platycodon grandiflorum with at least one or more of herbs chosen among Trichosanthes kirilowii MAXIM., arrowroot, Liriopis tuber, Angelica gigas Nakai, ginger, Schizandra chinesis BAILL., Citrus aurantium L., Citrus unshiu MARKOVICH., Prunus armeniaca l., Lonicera japonica THUNB., Perilla frutescens var., Astragalus membranaceus Bunge, Mentha arvensis L., Coicis Semen and Adenophora triphylla var.; (ii) heating the mixture in water at 100deg.C for 2hr or shaking in an organic solvent for 3days to get the extract; (iii) adding 0.01-55wt% of food additive such as herb juice, vitamin, amino acid, flavoring agent, sweetening agent, acidulant, stabilizer, preservative, salt, mineral or nutrition source into the extract; and then (iv) producing the resulting extract into tea, capsuled or concentrated liquid agent or functional drink.

Description

Functional food containing extract of bellflower extract and method for preparing the same {Functional food containing extract of Platycodon grandiflorusjacqin A De Candolle and process and for preparation Technical}

The present invention relates to a functional food containing the extract of bellflower and a method for producing the same. More specifically, the present invention relates to a functional food for treating bronchial diseases, and a method for producing the same, comprising a proper amount of a food additive in bellflower extract prepared by adding various herbal medicines to bellflower.

Bellflower has been used a lot for medicinal and edible foods since ancient times. It is cooked as a food that adds a unique bitter taste and aromatic effect because it contains a large amount of sugar, calcium and iron. According to the record of Dongbogam and herbaceous wood, medicinal bellflower is shown to be effective for respiratory system diseases, and also has anti-inflammatory, hemolytic and anti-complementary activities.

The inventors have extracted extracts from the bellflower to induce pneumonia and pulmonary tuberculosis Corynebacterium diphtheriaCorynebacterium diphtheriae) And Aspergillus pericatusAspergillus fumicatusThe present invention was completed by injecting into) and finding excellent antimicrobial effect of inhibiting bacterial growth and preparing functional foods.

Therefore, an object of the present invention is to provide a functional food for treating bronchial disease containing bellflower extract. Another object of the present invention to provide a method for producing a functional food for treating the bronchial disease.

The object of the present invention is to investigate the effect of bronchial disease treatment of the functional food through animal experiments after preparing a functional food by combining various herbal medicines, sweeteners and acidulants to the optimal content of the bellflower extract for effective bronchial disease treatment It was achieved by investigating the antimicrobial effect on various bacteria that invade bronchial tissue and cause secondary bronchial diseases.

Hereinafter, the configuration and operation of the present invention.

1 is a graph showing the survival rate (%) of the antimicrobial effect of bacteria on the type of bellflower extract added to the functional food containing the bellflower extract of the present invention.

The present invention comprises the steps of directly purchasing three-year-old root from the farmhouse in the Gyeonggi-si Gyeongju Gyeongsangbuk-do, dried, powdered and then hot water extraction and organic solvent extraction; Preparing the functional food by mixing the extract with herbal medicines, amino acids, vitamins, sweeteners, acidulants, natural juices, mineral iron salts, dietary fiber, caffeine, stabilizers and preservatives; Examining the bronchial disease treatment effect and antibacterial activity of the functional food prepared.

The bellflower used in the present invention was directly selected and purchased for 3 years from the farmhouse, which was grown in the Gyeonggi Gyeongju Gyeongsangbuk-do.

In the present invention, the herbal medicines were used alone or in combination of two or more of fruit fruit, brown root, control, pulse, donggi, ginger, Schisandra chinensis, fruiting, dermis, almond, honeysuckle, leaflet, Astragalus, peppermint, Euiin, Samsam and the like.

Natural fruit juice in the present invention was used alone or in combination of two or more quince, pear, pineapple, lemon, citrus, apricot, strawberry, peach, plum, watermelon.

In the present invention, L-cystine, L-tryptophan, L-phenylalanine, DL-methionine, L-valine, L-aspartic acid and L-glutamic acid were used alone or in combination.

In the present invention, vitamins were used alone or two or more vitamins A, B, C, D, E.

In the present invention, the inorganic material was used alone or in combination of two or more kinds of calcium citrate, calcium carbonate, calcium pantothenate, calcium gluconate, vegetable calcium, animal calcium, and the like as the calcium salt.

In the present invention, iron salts were used alone or in combination of two or more.

In the present invention, the nutritional source used honey or royal jelly.

In the present invention, the dietary fiber was used alone or in combination of two or more kinds of extracts of various useful plants such as aloe and chicory, various gums such as xanthan gum, dextrin, soluble fiber and the like.

In the present invention, sweeteners were used alone or in combination of glucose, honey, fructose, fructooligosaccharide, isomaltooligosaccharide, malto oligosaccharide chitosan oligosaccharide, chito oligosaccharide, isomerized sugar, aspartame, stevioside, sorbitol, mannitol.

In the present invention, the acidulant was used alone or in combination of two or more of DL-apple acid, succinic acid and citric acid.

In the present invention, the stabilizer was used alone or in combination of two or more polysorbate 20, polysorbate 40, polysorbate 80 in the sorbate.

In the present invention, the flavoring agent was used alone or in combination of two or more mixed fruit flavor, apple flavor, yogurt flavor, drink flavor.

In the present invention, the preservatives were used alone or in combination of two or more of benzoic acid and derivatives thereof, dehydroacetic acid and salts thereof, sorbic acid and salts thereof.

The amount of bellflower extract in the functional food prepared in the present invention is 0.01 to 40% by weight relative to the total functional food composition ratio, preferably 0.1 to 10% by weight.

Hereinafter, the specific method of the present invention will be described in detail with reference to Examples and Experimental Examples, but the scope of the present invention is not limited to these Examples and Experimental Examples.

Example 1 Production of Hot Water Extract of Bellflower

In this embodiment, in order to produce a bronchial disease-treating substance from bellflower, 1.0-200 g of bellflower, 1.0-50 g of fruit juice, 1.0-50 g of brown root, 1.0-20 g of black root, 1.0-50 g, pulmonary sinus 1.0-50 g, donkey 1.0 -50 g, ginger 1.0-20 g, schizandra 1.0-50 g, fruit fruit 1.0-20 g, dermis 1.0-30 g, almond 1.0-50 g, phosphorus 1.0-20 g, leaflet 1.0-20 g, astragalus 1.0-30 g, mint 1.0-20 g, extract ~ 60g, 1.0-3g of four ginsengs were mixed alone or in mixture of two or more, and heated at 100 ° C. for 2 hours, followed by hot water extraction to obtain 300 mL of a supernatant.

Example 2 Production of Organic Solvent Extract of Bellflower

In this example, in order to produce bronchial diseases from the bellflower, 1 liter of organic solvents, ethyl ether, ethanol and petroleum ether (special reagent), 1.0 to 200 g of bellflower, 1.0 to 50 g of fruit juice, 1.0 to 20 g of root, 1.0 to 20 g of control, 50g, McMoondong 1.0-50g, Angelica 1.0-50g, Ginger 1.0-20g, Schisandra 1.0-50g, Fruit fruit 1.0-20g, Dermis 1.0-30g, Almond 1.0-50g, Phosphorus 1.0-20g, Small leaf 1.0-20g, Astragalus 1.0- 30 g, peppermint 1.0-20 g, Uiyi 1.0-60 g, samsam 1.0-100 g, alone or in mixture of two or more, shaken for 3 days, eluted and concentrated under reduced pressure using a boiling point of an organic solvent. 300 mL of extract was obtained.

Example 3 Preparation of Healthy Tea Containing Hot Water Extract of Bellflower

To 300 mL of the hot water extract of bellflower obtained in Example 1 in the present Example, vitamin C 0.001-1.0 wt%, aspartic acid 0.01-5.0 wt%, potassium citrate 0.01-1.0 wt%, nicotinic acid amide 0.001--5 wt%, 1 to 20% by weight honey, 0.1 to 5.0% by weight quince juice, 0.01 to 50% by weight pear juice, 0.01 to 3.0% by weight polysorbate, 0.1 to 10% by weight fructooligosaccharide, 0.05 to 5% by weight citric acid Was made.

The mixture was lyophilized and powdered to prepare a powder.

Example 4 Preparation of Dietary Supplement Containing Hot Water Extract of Bellflower

In the present Example, the bellflower extract obtained in Example 1 was added in the same manner as in Example 3 to prepare a mixture. That is, in 300 mL of the extract obtained in Example 1, vitamin C, aspartic acid, potassium citrate, nicotinic acid amide, honey, quince juice, benzoic acid, pear juice, polysorbate, fructooligosaccharide, citric acid in the same amount as in Example 3 Was added.

The mixture was concentrated to encapsulate and concentrate to prepare as a functional health supplement.

Example 5 Preparation of Healthy Drink Containing Hot Water Extract of Bellflower

In the present Example, the bellflower extract obtained in Example 1 was added in the same manner as in Example 3 to prepare a mixture. That is, in 300 mL of the extract obtained in Example 1, vitamin C, aspartic acid, potassium citrate, nicotinic acid amide, honey, quince juice, benzoic acid, pear juice, polysorbate, fructooligosaccharide, citric acid in the same amount as in Example 3 Was added.

0.05 to 1% by weight of drink flavor and 0.05 to 1% by weight of benzoic acid were added to the mixture and sterilized at 100 ° C. for 2 hours to prepare a functional health beverage.

Example 6 Preparation of Healthy Tea Containing Organic Solvent Extract of Bellflower

In the present embodiment was prepared in the same manner as in Example 3 health tea, using the bellflower organic solvent extract obtained in Example 2.

Example 7 Preparation of Dietary Supplement Containing Organic Solvent Extract of Bellflower

In the present embodiment was prepared in the same manner as in Example 4 capsules and concentrate in the form of a health supplement, using the bellflower organic solvent extract obtained in Example 2.

Example 8 Preparation of Healthy Drink Containing Organic Solvent Extract of Bellflower

In the present embodiment was prepared in the same manner as in Example 5 functional health drink, using the bellflower organic solvent extract obtained in Example 2.

Experimental Example 1: Evaluation of Functionality of Food Containing Bellflower Extract

In this experimental example, the main functionalities of the functional foods prepared in Examples 1 to 8 were evaluated by animal experiments. For functional evaluation, 6-week-old S · D male rats (160 g) were purchased and adapted to solid feed (Samyang feed) for 1 week. The breeding room was lit and turned off every 12 hours while maintaining a temperature of 20 ° C. ± 5 and a humidity of 60%. The experimental animals were divided into the control group and the experimental group. The control group was a healthy rat without inhalation of tobacco smoke and bacterial infection. Rats of the experimental group were put in a closed box (L90 × W45 × H45cm), inhaled tobacco smoke for 1 day, and then injected with pneumonia bacteria into bronchial tissues to induce bronchial disease and then reacted for 1 day. The criteria for functional evaluation were interpreted as pathological findings. When bronchial tissue is induced and inflamed, leukocytosis (2.5 × 10 ⁴ or more) is shown in which the leukocyte count is increased than in a healthy state (5 × 10 ³ to 2.5 × 10 ⁴ ). Functional evaluation was performed. In order to determine whether bronchial disease was induced, blood was collected from healthy control rats and experimental rats immediately before treatment to measure leukocyte count. In the experimental group induced bronchial disease, rats with similar body weight were randomly divided into the control group and the experimental group. The control group was orally administered 0.5 mL (100 mL per 60 kg) of distilled water per 100 g to give the same stress as the experimental group. In the experimental group, 0.5 mL (based on 300 mL per 60 kg) of Bellflower extract sample per 100 g body weight was orally administered every 5 hours. Leukocyte count was collected from rat tail vein at 5 hour intervals for 3 days, diluted with dye solution mixed with methylene blue in 0.3% acetic acid, Turk's solution, and then hemocytometer (Sigma Chemical Co., Ltd.). ) Was used to calculate the leukocyte count in blood of 1 mm ³ . The calculation method is as follows.

Leukocyte count (pcs / 1mm ³ ) = d × V × 250

Where d is the mean number of leukocytes in 1 mm ³ , V is the dilution factor and 250 is 1 mm ³ ÷ 0.004 mm.

The results of examining the effect of the present invention on the treatment of bronchial diseases are shown in Table 1. The control group is a healthy rat and no addition is a control group of the experimental group treated with water. The sample is a solution extracting only the bellflower (hereinafter, called "flower bellflower extract"), and the addition is a solution extracted from the bellflower and various herbal medicines (hereafter bellflower extract). Company A's product is a mixture of bellflower and 17 kinds of herbal medicines. Sample A is bellflower extract 67g / L, sample B is 34g / L, and sample C is 20g / L. Addition 1 is the bellflower extract 67g / L, Addition 2 is 40g / L and Addition 3 is manufactured by A company bellflower extract 63g / L or more is added. The effect on the treatment of bronchial diseases showed the effect of recovering to normal state in all kinds. Compared with the A company with addition 3, all were excellent, and sample C was the best in addition to addition 1 in the sample.

Functional Evaluation of Food Containing Bellflower Extract: Animal Experiment Prototype Control No addition Sample A Sample B Sample C Addition 1 Addition 2 Addition 3 Weight (g) 260 ± 5 250 ± 2 250 ± 1 250 ± 2 250 ± 1 260 ± 2 260 ± 5 260 ± 5 White blood cell count (piece / 1mm ³ ) 4.9 × 10 ³ 5 × 10 ⁴ 1.6 × 10 ⁴ 9.6 × 10 ³ 6.9 × 10 ³ 5.1 × 10 ³ 9.4 × 10 ³ 2 × 10 ⁴ Lung weight (g) 1.324 1.2 1.668 1.227 1.260 1.239 1.201 1.211 Airway weight (g) 0.116 0.119 0.116 0.116 0.116 0.116 0.116 0.114 [Note] Rat white blood cell normal level: 5.0 × 10 ³ ~ 2.5 × 10 ⁴

Experimental Example 2: Evaluation of Functionality of Food Containing Bellflower Extract

In this experimental example, the main functionalities of the functional foods prepared in Examples 1 to 8 were evaluated as antimicrobial effect against bronchial disease-inducing bacteria such as asthma, pneumonia, pulmonary tuberculosis and bronchitis. Bacteria used in the experiments are commonly isolated from the sputum of patients with advanced bronchial disease. Aspergillus fumicatus , Mycobacterium sp ., Staphylococcus aureus , Klebsiella pneumoniae , Corynebacterium diphtheriae , Streptococcus pyogenes , Neisseria gonorrhoeae , Fusobacterium bacterium nucleatum nucleium nucleum Antimicrobial effect of inhibiting the growth of bacteria after cultivation by mixing 8 species of cultivated with bellflower extract in the bacterial culture medium (Blood broth: Difco Co.,). Was determined. The content of bellflower extract was the same as Experimental Example 1.

Table 2 shows the antimicrobial effect of the present invention on bronchial disease-inducing bacteria. The control means that the bellflower extract is not included in the culture medium for bacteria. The antimicrobial effect of bacteria that secondary to bronchial tissue causing bronchial disease is shown in Cortex B.Corynebacterium diphtheriaeExcept for It was excellent in all kinds. Particularly, in the sample using only the bellflower extract, A was the most excellent in the addition using the bellflower extract.

Functional Evaluation of Food Containing Bellflower Extract: Bacterial Experiment Prototype fungi Control Sample A Sample B Sample C Addition 1 Addition 2 Addition 3 Aspergillus pericatus (A. fumicatus) 0% 93.5% 72.4% 32.8% 83.0% 57.9% 81.5% Ella greater repji pneumoniae (K.pneumoniae) 0% 84.2% 97.0% 48.3% 74.3% 61.3% 41.7% Staphylococcus aureus 0% 99.0% 83.0% 11.0% 75.4% 43.2% 42.5% Mycobacterium sp. 0% 99.2% 74.0% 18.0% 80.0% 49.0% 62.7% Corynebacterium diphtheria (C. diphtheriae) 0% 99.5% 82.0% 8.0% 71.8% 39.9% 79.8% Streptococcus piogenes 0% 78.9% 97.0% 48.3% 88.0% 68.2% 69.2% N. gonorrhoeae 0% 81.6% 94.0% 43.5% 87.8% 65.7% 62.1% Fusobacterium nucleatum 0% 95.0% 82.0% 28.0% 88.5% 58.3% 71.8%

As described through the above Examples and Experimental Examples, the present invention bellflower functional foods prepared by combining the herbal medicine and food additives in 1.0 ~ 50% by weight of the extract of bellflower to relieve the symptoms and cold lung symptoms caused by cold and damaged bronchial tissue Health and functionality as it has an excellent effect of increasing the body's immune activity, such as anti-inflammatory action for the secondary infectious bacterial bronchitis disease, relief of sputum and relieving symptoms, treatment of asthma and lung disease caused by pollution and smoking It is a very useful invention in the food industry.

Claims (4)

Hydrothermal extraction or ethyl by mixing any one or more selected from the group of herbaceous herbs consisting of fruit, fruit, ginger, Schizandra chinensis, fruit, dermis, almond, honeysuckle, lobule, Astragalus, peppermint, uiyiin and samsam Functional food for the treatment of bronchial diseases containing extract obtained by extraction with ether, ethanol or petroleum ether. The functional food for treatment of bronchial diseases containing bellflower extract according to claim 1, wherein the functional food is tea, capsules, and concentrates or health supplements. a) Mix the herbaceous herbs, roots, roots, contrasts, pulses, donkeys, ginger, Schisandra chinensis, fruit, dermis, almonds, honeysuckle, leaflets, Astragalus, peppermint, Uiin and samsam, at least 100 Heating for 2 hours to extract hot water or extracting the mixture by shaking for 3 days in an organic solvent; b) bellflower extract containing functional food manufacturing method comprising the step of preparing by adding 0.01 to 55% by weight of the food additive to the extract obtained in step a). 4. The functional food for treating bronchial diseases containing bellflower extract according to claim 3, wherein the food additive is natural herbal juice, vitamin, amino acid, flavoring agent, sweetener, acidulant, stabilizer, preservative, salt, mineral or nutrient source. Manufacturing method.