KR20010056177A - Skin care composition - Google Patents

Abstract

PURPOSE: A skin-protecting cosmetic composition containing α-hydroxy acid and an epidermal growth factor as a component for improving the effect of a skin improving component is provided, which effectively reduces skin irritation and has improved skin improving effect. CONSTITUTION: The cosmetic composition for protecting the skin contains α-hydroxy acid and 0.00001 to 1%(preferably 0.0001 to 0.1%) by weight of an epidermal growth factor based on the total weight of the cosmetic composition, wherein the epidermal growth factor is prepared from recombined E. coliJM101.

Description

Skin care cosmetic composition

The present invention relates to a cosmetic composition containing epidermal growth factor (EGF), which is an epidermal growth factor, and removes keratin freckles and dry skin due to bleeding freckles and dry environment, which is one of the aging phenomena, or improves the skin by peeling the skin. It relates to a skin care cosmetic composition to minimize the skin irritation that appears in.

The skin, which consists of the epidermis, the dermis, and the subcutaneous fat, is the largest organ that makes up the human body. It is a very important organ that plays various roles such as protective function, barrier function, temperature control function, excretory function, and respiratory function. However, as the skin ages, the function of the skin decreases rapidly and ages as various changes occur. The thickness of the skin decreases, the function of the skin barrier decreases rapidly, and the moisture content of the skin decreases, and the skin dries. In addition to getting sunburn, it causes various skin lesions. In particular, highly reactive free radicals and free radicals generated by increased UV radiation, air pollution, and excessive fatigue and stress in modern people can oxidize or denature biological components (proteins, nucleic acids, cell membrane lipids, etc.) It acts as the main cause. Wrinkle formation, blemishes, freckles, skin elasticity reduction, and pigmentation due to skin aging are most frequently studied as cosmetics in the cosmetic field.

In dermatology, vitamin A retinol and alpha hydroxy acid (AHA) have been used for a long time to improve wrinkles, blemishes, freckles and pigmentation. It is widely used. In particular, the alpha hydroxy acid-containing cosmetic composition which is effective for exfoliation has been widely used to improve skin by promoting the activity of skin cells along with the exfoliation of the skin, but due to problems such as skin irritation, its concentration is used. Is strictly regulated.

In the case of alpha hydroxy acid, ancient Egyptians used it to bathe lactic acid, which is produced when fermented milk, a type of alpha hydroxy acid, to keep skin healthy and beautiful. The aristocratic women wore old wine on their faces to keep their skin soft and clean, and the old wine also contained an alpha hydroxy acid, malic acid and tartaric acid. Alpha hydroxy acid has been used for a long time. Since a patent (USP4105783) has been registered by E.J. Scott on the prevention and improvement of pigmentation such as anti-aging, acne improvement, blemish and freckles (clinical experiment), a number of related patents have been registered.

AHA is an abbreviation of Alpha Hydroxy Acid. Chemically, AHA is a generic name for substances having an alcohol group or a hydroxyl group attached to the carbon at the α position of Carboxylic acid.

The main physiological action of AHA is to lower the ion binding energy of keratinocytes to remove the dead skin cells or to promote the formation of new cells, which shortens the cycle of keratinization process as the skin ages. In addition, AHA has a function of promoting the production of collagen and elastin by Fibroblast, making skin elastic and soft, and promoting the production of Mucopolysaccharide, an important extracellular matrix component of dermis.

Currently, the most widely used AHAs are lactic acid (Lactic acid, MW = 90.08) obtained by fermented milk or enzymatic reaction, citric acid (MW = 210.14), apples, grapes, Malic acid (Malic acid, MW = 130.98) contained in a large amount of strawberries and tartaric acid (MW = 150.09) obtained from grapes. In particular, lactic acid is most commonly used in cosmetics because it has excellent cell regeneration effect, moisture control effect, and exfoliation effect but low skin irritation in AHA. Glycolic acid (MW = 76.05) obtained from sugar cane has the smallest molecular weight among AHAs, so it penetrates the skin quickly.

Therefore, various studies are being conducted to solve the skin irritation of AHA these days, and the use of BHA is one example. BHA is an abbreviation of Beta Hydroxy Acid, and chemically refers to substances having a structure in which an alcohol group or a hydroxyl group is attached to the carbon at the β-position of carboxylic acid. Studies on BHA show that AHA is a low molecular weight organic acid (76.1-210.1), which easily penetrates into the skin and causes various skin irritation, whereas BHA has a higher molecular weight (105-194) than AHA. Although it does not penetrate easily, it has similar effect to AHA (skin exfoliation effect, but skin moisturizing effect, cell regeneration effect, whitening effect, wrinkle improvement effect, skin elasticity effect, etc.) are claimed to be less skin irritation. In fact, skin irritation caused by BHA is known to be quite large.

In the case of AHA, Glycolic acid was mainly used, but problems such as skin irritation problems and balanced peeling did not occur. In addition, by using AHA alone, problems such as AHA reacting with a substance in the skin and becoming a salt or being neutralized have decreased effects.

In addition, in order to have physiological activity, the acidity (pH) of the product should be between 2 and 3, which is accompanied by very strong irritation to the skin, that is, burning, redness, and dry skin. Therefore, by encapsulating AHA in the form of liposomes and formulating it between pH 5-6, skin irritation was almost reduced. Therefore, these days, various kinds of fruit acid (AHA) and its derivatives are combined to create an AHA Complex, and various attempts have been made to minimize skin irritation caused by acidity (pH 3.8-4.0) and to increase the effect. However, there is still a problem about skin irritation and research is required to solve it.

In general, AHA is used to less than 10% in cosmetics according to the concentration, thereby reducing the cohesion between keratinocytes to remove keratin or improve pigmentation, such as blemishes, freckles and remove acne. In addition, AHA has been used to activate fibroblasts to remove fine wrinkles or improve dry skin caused by an increase in the amount of collagen. In addition, AHA with a high concentration of 30% or more has been used for a long time for the special treatment of peeling agent and dermatology (pigmentation skin, aging skin treatment) by specialist. However, the AHA-containing cosmetics used in various ways have a problem as a cosmetic that the higher the concentration of AHA, the higher the acidity (pH), the greater the beauty effect of the skin, but the skin irritation increases relatively.

An object of the present invention by improving the problems of the conventional cosmetic ingredients, that is, AHA, to remove the dead skin, improve pigmentation, such as blemishes, freckles, or to remove the acne, while maintaining the efficacy as a cosmetic, while also irritating the skin It is to provide a cosmetic composition that does not exhibit such side effects.

In order to achieve the above object, the present invention is carried out to provide a cosmetic composition containing Epidermal Growth Factor (EGF) as a component to alleviate the skin irritation of these components together with the existing skin improvement component AHA. .

The present invention relates to a skin care cosmetic composition containing EGF together with skin improvement ingredients such as AHA, which are used for the purpose of removing wrinkles and keratinous skin, removing blemishes, freckles, and improving pigmentation.

The inventors have found that Epidermal growth factor (EGF), an epidermal growth factor, effectively alleviates the skin irritation of AHA-containing therapeutic external preparations and cosmetics that are widely used to improve skin wrinkles, blemishes, freckles and pigmentation. The present invention has been completed.

EGF is a potent cell division promoter of various epithelial cells originating from ectoderm and mesoderm. It is a single polypeptide with 53 amino acid residues widely distributed in body fluids and present in high concentrations in urine and breast milk (Capenter, 1985). Is 6,200 daltons (Capenter and Cohen, 1979: Capenter and Wahl, 1990). In 1962, Cohen isolated and identified Cohen's submandibular gland in mature rats, and in 1972 Savage and Taylor identified three intramolecular disulfide bonds that play an essential role in the primary structure and physiological action of Mouse EGF. . Human epidermal growth factor (Human EGF, hEGF) was confirmed in 1975 by the same structure as Urogastron human EGF, a gastric secretion hormone derived from Gregory's urine. EGF acts as a powerful promoter for cell proliferation of epithelial cells, endothelial cells and fibroblasts, and has an excellent wound healing effect because it promotes the migration and proliferation of epithelial cells during epithelial cell defects. EGF used in the present invention was purified and purified from recombinant Escherichia coli and proved to be identical to human hEGF. (US5,652,120: JP2609515: EP652954: KR102993: KR107023: KR110123: KR114856)

EGF used in the present invention is contained in an amount of 0.00001 to 1% by weight, preferably 0.0001 to 0.1% by weight relative to the total weight of the cosmetic, AHA is 0.1 to 20% by weight, preferably based on the total weight of the cosmetic It is contained in the amount of 3.0-10.0 weight%.

EGF used in the present invention is purified by fermenting the recombinant E. coli ( E. coli JM101) for 48-72 hours by a fed-batch method by pure separation of the fermentation supernatant by Amberchrome CG71 chromatography and Q-sepharose chromatography. And manufactured. (US5,652,120: JP2609515: EP652954: KR102993: KR107023: KR110123: KR114856)

The cosmetic composition of the present invention is not particularly limited in the formulation, for example, softening longevity, astringent longevity, nourishing longevity, eye cream, nutrition cream, massage cream, cleansing cream, cleansing foam, cleansing water, powder, essence It may have a dosage form, such as a pack.

In addition, in the cosmetic composition of each formulation, ingredients other than AHA (glycolic acid, tartaric acid, Citric acid, lactic acid, malic acid) or BHA as the raw material for skin improvement including the EGF described above, etc. Therefore, those skilled in the art can combine the formulations appropriately without difficulty.

Hereinafter, a test example will be described in detail the configuration and effects of the present invention, but the present invention is not intended to be limited to these test examples.

For the test example, according to the present invention, a composition containing EGF with AHA (lactic acid) (Example) and a composition containing only AHA (Comparative Example) were prepared as shown in Table 1 below.

Table 1.

Examples and Comparative Examples

Unit (part by weight) Ingredient Example Comparative Example EGF 0.005 - Lactic acid (AHA) 10.0 10.0 vaseline 7.0 7.0 Liquid paraffin 10.0 10.0 Beeswax 2.0 2.0 Polysorbate 60 2.0 2.0 Sorbitan sesquioleate 2.5 2.5 Squalane 3.0 3.0 Propylene glycol 6.0 6.0 glycerin 4.0 4.0 Triethanolamine 0.5 0.5 Carboxy Vinyl Polymer 0.5 0.5 Tocopheryl Acetate 0.1 0.1 Incense, preservative a very small amount a very small amount Purified water to 100 to 100

Test Example 1 Patch Test

A skin patch test was performed on the EGF-containing skin protective cosmetic of the present invention.

After dividing 30 subjects (mean age 22 years, age distribution 19-34 years) into 2 groups (A, B), the group A was compared to the examples, and the group B was compared to the comparative examples. After the formulation according to Table 1, using a Haye's Test Chamber was carried out a skin patch test (Patch Test). However, Psoriasis, Eczema, and other skin lesion holders, or those who are pregnant, nursing or contraceptives, and antihistamines, were excluded from this study.

After wiping the test site with 70% ethanol and drying, the cream of Example was added to the group A and the cream of the comparative example to the group B was added dropwise to the chamber by 15 µg, respectively, and then fixed on the upper region of the test site. The patch was applied for 24 hours, and after removing the patch, the test site was marked with a marking pen, and the test site was observed after 24 hours, 48 hours, and 72 hours, respectively.

Determination was performed 24 hours, 48 hours, 72 hours after patch removal, and skin reactions were determined according to the regulations of the International Contact Dermatitis Research Group (ICDRG) shown in Table 2 below. The test results are shown in Table 3 below.

Table 2.

Criteria for international contact dermatitis

sign Criteria evaluation Mean score ± ++++++- Doubtful or slight reaction and erythemaErythema + IndurationErythema + Induration + VesicleErythema + Induration + Bullae Stimulus stimulus non stimulus 0 to 0.91.0 to 2.93.0 to 4.95.0 or more

Table 3.

Skin Patch Test

Elapsed time Example Comparative Example 24 hours 0.5 4.5 48 hours 0 2.0 72 hours 0 0.5

As a result of the skin safety test according to the skin patch test, the comparative example containing only AHA showed very strong irritation at 24 hours, 48 hours and 72 hours, whereas the example containing AHA and EGF had very low microstimulation only in the first 24 hours. But no stimulus after 48 hours. This indicates that EGF effectively inhibits skin irritation caused by AHA. In general, one of the main functions of AHA is exfoliation. When AHA is applied to the skin, the skin becomes very thin. Therefore, it is thought that EGF is rapidly regenerated (3-4 days) over time and the skin thickness is restored to normal by thinning the skin effectively, thereby reducing the skin irritation.

[Test Example 2] Skin irritation test (Stinging Potential Test)

Skin irritation test was performed on the EGF-containing skin protection cosmetic of the present invention.

For 20 subjects (mean age 22 years, 19-34 years of age distribution), a sufficient amount of sweating was performed using a steam generator for 15 minutes, followed by an example on the left face and a comparative example on the right face. Rubbing strongly around. Stinging grades were evaluated as classified as intense stinging within 30 seconds, mild stinging within 2.5 minutes, moderate stinging within 5 minutes, and delayed stinging within 8 minutes after application. Psoriasis, Eczema, and other skin lesion holders, or those who are pregnant, nursing or contraceptives, or antihistamines, are excluded from this study.

After applying the sample (Example, Comparative Example) to the face, the skin reaction (itch, itching, soreness, redness, swelling, etc.) observed for 8 minutes, and the response score to the number of subjects (n) Stimulation was evaluated by obtaining the response rate (%). Skin irritation degree according to the rate of stimulation was determined according to Table 4 below and the results are shown in Table 5.

Table 4.

Skin irritation test criteria

Grade Response rate (%) Judgment One 0.00-0.75 Non-irritating 2 0.76-1.50 Unstimulated 3 1.51-2.50 Light stimulation 4 2.51-4.00 Heavy stimulation 5 4.01 or more Strong stimulation

Table 5.

Skin irritation test result

Example Comparative Example Response rate (%) 0.95 4.55 Judgment Unstimulated Strong stimulation Grade 2 5

As a result of the skin safety test by the skin irritation test, it was determined that the response rate (%) was unstimulated with 0.95, but it showed a very low skin irritation compared to the comparative example (Response rate: 4.55) containing only AHA. . In general, considering that the cosmetics containing AHA have a strong irritation, it can be seen that the cosmetics of the embodiment containing EGF are very safe cosmetics.

Test Example 3 Stability Test

The stability test of the formulation for the said EGF containing skin care cosmetics of this invention was done.

To test the stability of the cosmetics, the examples and comparative examples were stored in an opaque glass container in a constant temperature bath at 45 ° C. for 12 weeks, and also opaque in a completely shaded refrigerator kept at 4 ° C. After storing for 12 weeks in a glass container, the degree of separation and discoloration were measured. The results are shown in Table 6 below. At this time, the degree of product separation or discoloration was evaluated by classifying into the following six grades.

Product discoloration evaluation criteria

0: no change 1: very little separation (discoloration)

2: Separate slightly (discolored) 3: Separate slightly (discolored)

4: severely separated (discolored) 5: extremely severely separated (discolored)

Table 6.

Separation and discoloration

Temperature Discoloration degree of test substance Example Comparative Example 45 ℃ 0 0 4 ℃ 0 0

As a result of the formulation stability test, it can be seen that there is no discoloration and separation in the Examples and Comparative Examples, and thus it is a stable base.

According to the results of the above test examples, skin irritation that occurs when using alpha hydroxy acid (AHA), which is widely used in therapeutic external preparations and cosmetics to improve skin wrinkles, blemishes, freckles, pigmentation, and epidermal growth factor Phosphorus Epidermal growth factor (EGF) was found to be effectively alleviated by using together, it was also possible to provide an EGF-containing composition of the present invention to alleviate the skin irritation of the existing cosmetics.

Hereinafter, based on the results of the above test examples, the formulation examples of the present invention containing EGF will be presented. However, the composition of the present invention is not intended to be limited to the following formulation examples.

Formulation Example 1 Softening Cosmetic (Skin Lotion)

Ingredient Parts by weight Lactic acid 0.1 EGF 0.0001 1,3-butylene glycol 6.0 glycerin 4.0 Oleyl alcohol 0.1 Polysorbate 20 0.5 ethanol 15.0 Benzophenone-9 0.05 Incense, preservative a very small amount Purified water to 100

Formulation Example 2. Nutrients (Milk Lotion)

Ingredient Parts by weight Lactic acid 2.0 EGF 0.0002 Propylene glycol 6.0 glycerin 4.0 Triethanolamine 1.2 Tocopheryl Acetate 3.0 Floating paraffin 5.0 Squalane 3.0 Macadamia Nut Oil 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.0 Carboxyvinyl Polymer 1.0 Incense, preservative a very small amount Purified water to 100

Formulation Example 3. Nutrition Cream

Ingredient Parts by weight Lactic acid 5.0 EGF 0.0005 vaseline 7.0 Liquid paraffin 10.0 Beeswax 2.0 Polysorbate 60 2.0 Sorbitan sesquioleate 2.5 Squalane 3.0 Propylene glycol 6.0 glycerin 4.0 Triethanolamine 0.5 Carboxy Vinyl Polymer 0.5 Tocopheryl Acetate 0.1 Incense, preservative a very small amount Purified water to 100

Formulation Example 4 Massage Cream

Ingredient Parts by weight Lactic acid 2.0 EGF 0.0002 Propylene glycol 6.0 glycerin 4.0 Triethanolamine 0.5 Beeswax 2.0 Tocopheryl Acetate 0.1 Polysorbate 60 3.0 Sorbitan sesquioleate 2.5 Cetearyl Alcohol 2.0 Liquid paraffin 30.0 Carboxy Vinyl Polymer 0.5 Incense, preservative a very small amount Purified water to 100

Formulation Example 5 Pack

Ingredient Parts by weight Lactic acid 10.0 EGF 0.0005 Propylene glycol 2.0 glycerin 4.0 Carboxyvinyl Polymer 0.3 ethanol 7.0 Fiji-40 Hydrogenated Castor Oil 0.8 Triethanolamine 0.3 Incense, preservative a very small amount Purified water to 100

As described above, EGF contained in the cosmetic composition of the present invention was able to effectively alleviate the skin irritation of AHAs widely used in external preparations or cosmetics for improving skin wrinkles, blemishes, freckles and pigmentation.

As the EGF-containing skin protection cosmetic composition of the present invention is completed, the cosmetic composition with reduced skin irritation can be provided while still maintaining the existing skin wrinkles and blemishes, freckles, and pigmentation improvement effects.

Claims (4)

A skin protective cosmetic composition, characterized in that it contains Epidermal Growth Factor (EGF) together with Alpha Hydroxy Acid. The method of claim 1, The epidermal growth factor is contained in an amount of 0.00001 to 1% by weight based on the total weight of the cosmetic, skin protection cosmetic composition. The method according to claim 1 or 2, The epidermal growth factor is contained in an amount of 0.0001 to 0.1% by weight based on the total weight of the cosmetic, skin protection cosmetic composition. The method according to claim 1, wherein Epidermal growth factor is characterized in that produced from recombinant E. coli ( E. coli JM101), skin care cosmetic composition.