JPS60258105A - Cosmetic - Google Patents

Abstract

PURPOSE:Cosmetic having raised water retention effect on the skin, making the skin smooth, improving chapped skin, preventing fine wrinkles, containing an epidermal growth factor, a single chain polypeptide found in glandula submandibularis of mouse. CONSTITUTION:Cosmetic such as toilet lotion, cream, milky lotion, lipstick, etc. containing 0.001-5.0wt%, preferably 0.005-2.0wt% epidermal growth factor, an acidic single chain polypeptide containing 53 amino acid residues, having 6,045 mol.wt., and a compound having N end of asparagine, C end of arginine, and 6 cysteine residues. In its constituent amino acids, lysine, alanine, and phenylalanine do not exist, and the compound is a polypeptide showing resistance to trypsin and having high heat stability, and is easily purified.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention provides epidermal growth factor (EpiaermalGr)
The present invention relates to cosmetics containing owth facjor (hereinafter abbreviated as EGF).

KGF is a single-chain polypeptide discovered from mouse submandibular gland by Niss Cohen (S, 0oben) [Journal of Biological Chemistry (J, 131o1. Chew, ), 237
．． 1555 (1962)), the structure of which was revealed by C.R. Savage et al.
7. 7612 (1972)). That is, EGF is
Molecular weight with 53 amino acid residues6. It is an acidic single-chain polypeptide of O45, and has six cysteine residues, with asparagine at the N-terminus and arginine at the C-terminus.

Furthermore, it is a highly thermostable polypeptide that is resistant to trypsin and does not contain lysine, alanine, or phenylalanine among its constituent amino acids. Since the purification method is relatively easy, many studies are being conducted.

EGF promotes the opening of the eyelids and the appearance of incisors in neonatal mice, and experiments using cultured cells have shown that it promotes the differentiation of epidermal cells, extends the lifespan of cells, and also promotes the development of skin fibroblasts. It has been reported that it promotes proliferation. One of these! The pharmacological effects of iGF have attracted attention, and its use as a partial replacement for serum in tissue culture and its application in pharmaceuticals is being considered, but no attempt has been made to incorporate it into cosmetics.

Conventionally, vitamins and placenta preparations have been used to improve rough skin and rough skin, but they have not been sufficiently effective in the essential sense of η. In addition, there is a method using female hormones, but this method is not satisfactory because excessive use may actually interfere with normal skin metabolism. In addition, in order to enhance the water retention effect before childbirth, polyib-14, which is made by hydrolyzing collagen, is used, but this is not completely satisfactory. The present inventors have conducted various studies on the formulation of EGF into cosmetics. As a result, the present invention was completed by discovering that the moisture retention effect before childbirth is enhanced, the skin becomes smoother, and excellent skin beautifying effects such as improvement of rough skin and prevention of fine wrinkles can be obtained.

According to the present invention, EGF is used together with various cosmetic base materials in the form of basic cosmetics such as lotions, milky lotions, and creams, makeup cosmetics such as lipstick, and the like. These cosmetics can be manufactured by conventional methods.

The content of KGF contained in the cosmetics of the present invention is usually 0.00
01 to 50% by weight, preferably 0005 to 20% by weight. If the amount of O, OO is less than 1% by weight, sufficient effects cannot be obtained. Moreover, if the amount exceeds 50% by weight, the effect will not be enhanced and it will be uneconomical.

The present invention will be described in detail below with reference to Examples. The content is expressed in weight%.

Example 1 Lotion A) Glycerin 50 Ethyl alcohol 50 Citric acid 005 Sodium citrate 0.1 EGF 0.05 Preservative Appropriate amount Fragrance Appropriate amount Purified water 898 Manufacturing method: Uniformly dissolve and mix each component to create the desired lotion I got it.

Example 2 Cream A) Stearic acid 5, O -: 1 Stearyl alcohol 1.0 Liquid paraffin 60 Vaseline 5° Ceresin wax 5.0 Glycerin monostearate 2.5 Sorbitan monostearate o5 E) 1,3-butanediol 5゜Potassium hydroxide 0.5 Preservative Appropriate amount Purified water 695 0) EGF 0.01 Flavor Appropriate amount Production method: After heating and dissolving component A and component B at 70 to 75°C, add B to A and emulsify. During cooling,
Ingredient 0 was added and mixed, and the mixture was cooled to 30°C to obtain the desired cream.

Example 3 Emulsion A) Stearic acid 5゜ Cetyl alcohol 5.0 Squalane 2.0 Sorbitan monooleate 1.5 Polyoxyethylene sorbicun monooleate 08 B) Glycerin 6.0 Triethanolamine 0.7 Preservative appropriate amount Purified water 777 0) EGF O,01 Fragrance (appropriate amount) Production method: The desired emulsion was obtained in the same manner as in Example 2.

Example 4 Lipstick A) Castor oil 40.0 Hexadecyl alcohol 300 Lanolin 50 Beeswax 50 Candelilla wax 60 Carnauba 4,0 Acid additive Preservative Appropriate amount B) Pigment base 8.5 0) EGF O, 0()5 Fragrance Appropriate amount Manufacturing method: Heat and dissolve component A and mix uniformly. Component B is added to this, and the mixture is kneaded using a p-lumyl to uniformly disperse it, then melted again and component 0 is added. After defoaming, it was poured into a mold and then rapidly cooled to harden to obtain the desired lipstick. Note that E'GF manufactured by Sigma was used.

In order to clarify the effects of the cosmetics of the present invention, a usage test was conducted on 40 general women between the ages of 25 and 50, and a questionnaire survey was conducted using a double-blind method, focusing on the skin conditioning effects. Table 1 summarizes the results of using a lotion containing EGF (the lotion of Example 1) and a conventional lotion that does not contain EGF (the formulation of Example 1 minus FiGIF) for two months. Ta. In addition, no skin troubles occurred during the use test period.

Table 1. Judgment results of skin conditioning effect

Claims (1)

[Claims] A cosmetic characterized by containing epidermal growth factor (EGF).