KR20010038100A - Cosmetic compositions for skin whitening containing extract of galenical medicines - Google Patents

Abstract

PURPOSE: A skin whitening cosmetic composition containing an excellent melanin inhibiting effect such as Psedo-Ginseng Radix, Ulmi pasta, Nardostachytis Rhizoma, Cirsii Radix, Kaempferiae Rhizoma and Ulmus davidiana extracts is provided which has excellent skin whitening efficacy without skin irritation. CONSTITUTION: This skin whitening cosmetic composition comprises one or more extracts selected from the group consisting of Psedo-Ginseng Radix, Ulmi pasta, Nardostachytis Rhizoma, Cirsii Radix, Kaempferiae Rhizoma, Ulmus davidiana and additionally Euphorbiae Lathyridis Semen and Curcumae Rhizoma.

Description

Cosmetic composition for skin whitening containing extract of galenical medicines}

The present invention relates to a cosmetic composition having excellent skin whitening effect without skin irritation, specifically, Psedo-Ginseng Radix, Ulmi pasta, Carpesii Fructi, Nardostachytis Rhizoma (Cirsii Radix), Kaempferiae Rhizoma and Ulmus davidiana, which contain one or more herbal extracts selected from the group consisting of, or known as a whitening effect, Euphorbiae Lathyridis Semen, Curcumae Rhizoma Or it relates to a whitening cosmetic composition further containing an extract of these mixtures.

In general, it is important to determine the black-and-white color of a person's skin color. Melanocite, which produces melanin pigments, is determined by various external products (ultraviolet rays, medications, irritating skin externals) and internal factors (hormones, inflammatory factors, and cytokines). melanocyte) activity and distribution. In melanocytes in the human body, enzymes such as tyrosinase act and produce a black pigment called melanin through an oxidation reaction based on an amino acid called tyrosine which is always present in a living body. This melanin pigment is widely distributed to the surrounding keratinocytes through the dendritic projections of the pigment cells, thereby determining the color of the skin.

Skin pigmentation disorders such as blemishes, freckles, and senile plaques are caused by abnormally activated melanin production in pigmented cells resulting in excessive production deposition. This hyperpigmentation can be a serious mental burden from a cosmetic point of view that can interfere with normal social activities. In addition, Asian women have long preferred white and fair skin, and have made it an important standard of beauty. There have been many developments of whitening agents for the treatment of dyskinesia and for satisfying cosmetic needs. Although substances with tyrosinase inhibitory activity, such as ascorbic acid, kojic acid, arbutin, hydroquinone, glutathione and derivatives thereof, have been formulated into cosmetics or medicines, cosmetics and medicines with whitening effects have been provided. B. When used in cosmetics or pharmaceuticals, its use is limited due to insufficient whitening effect.

In addition to these whitening materials, research has been conducted to find whitening active ingredients in natural products, especially plants, among them white pear skin (Japanese Patent Publication No. 55-44375, Small 64-26507, Small 64-83009, Pyeong 1-25687, Hei 5-139950 and Korean Laid-Open Patent Nos. 92-002109, 97-021273), Licorice (Japanese Laid-Open Patent Nos. 60-214721, 60-214728, 63-23809, 64-64506, Hei 1-149706 and Korean Laid-Open Patent 92-002109, 97-025601), Peony (Japanese Patent Laid-Open No. 61-246109 and Korean Patent Laid-Open No. 92-002111), Cinnamon (Japanese Laid-Open Patent No. 63-30403, Hei 5-139954), Gosam (Japanese Laid-Open Patent No. 64) -26507 and Korean Laid-Open Patent No. 92-002110), Brown root (Japanese Laid-open Patent No. 60-214727, Small 64-16709), Angelica (Japanese Laid-open Patent No. 56-92211, Hep. 4-26610), Mokpi (Japanese Laid-Open Patent No. 60 -214721, small 61-50915, small 61-246109), half (Japanese Laid-Open Patent Publication No. 2-207028, Korean Laid-Open Patent 96-033442), Aloe (Japanese Laid-Open Patent Publication No. 52-44375, Flat 2-207030, Flat 5- Many plant extracts And it has been found that extracts of herbal medicines act on tyrosinase to inhibit melanin production, but they also have many problems in using above the effective concentration in cosmetics or pharmaceuticals in terms of safety and discoloration, The situation is not effective.

Accordingly, the present inventors have searched to provide a new whitening material that can overcome the problems of the known whitening efficacy substances, and, as a result of the herbal medicine that can be said that the safety has been indirectly proved, Samchil-Keun, Muyi The present invention has been found to have a very good melanogenesis inhibitory effect of the extract of Gakseul, Hak Songhyang, Greater Root, Samgyeja or Yugeunpi.

These findings were first confirmed by a different method from conventional screening methods (measuring mushroom-derived tyrosinase activity, melanin determination using B16-melanoma cells), secondly by animals, thirdly by extracts, etc. It was possible to confirm the effect from the results of experiments that were directly applied to the human skin by preparing a cosmetic containing.

Accordingly, it is an object of the present invention to provide a cosmetic composition excellent in whitening efficacy without skin irritation.

The above and other objects, features and advantages of the present invention will become apparent to those skilled in the art from the following detailed description.

In order to achieve the above object, the whitening cosmetic composition of the present invention is characterized in that it contains one or more herbal extracts selected from the group consisting of Samchil root, radish, Hakseul, Gamsonghyang, geungeuk, Samnaeja and Yukeunpi.

In addition, the whitening cosmetic composition of the present invention is characterized in that it further contains extracts of water, turmeric or mixtures thereof.

Hereinafter, the present invention will be described in more detail.

Psedo-Ginseng Radix (Psedo-Ginseng Radix) is the root of the perennial herb, Oaliaceae, Panax notoginseng (Burk.) F.H.Chen.

Ulmi Pasta is a fruit of the deciduous tree, Ulmus macrocarpa Hance, used for the treatment of pain, digestion, and insect repellent.

Carpesii Fructi is a fruit of the two-year-old herbaceous plant, Carpesium abrotanoides, which is used to treat abdominal pain caused by insecticides and long-term insects.

Nardostachytis Rhizoma is the root of the perennial herb, Nardostachys chinensis Batalin, which is used for stomach pain, gastrointestinal cramps, chest thorax, neurogastrointestinal disorders, vomiting, headaches, and others.

Circii Radix is the root of the cactus Cirsium japonicum DC. It is used for the treatment of boils and for hemostasis such as uterine bleeding, hemorrhage, and nosebleeds.

Kaempferiae Rhizoma (Kaempferiae Rhizoma) is the root and stem of Ginger (Kaempferia galanga L.), which is used for the treatment of sambok cold, toothache, and vertigo.

Ulmus davidiana (Rlmus davidiana) is the root bark of the elm tree, coughing, astringent and anti-inflammatory.

The present invention has found that the extracts of Samchil-Geun, Radish, Hak-Seul, Saeng-Hyang, Dae-Geun-Geun, Samja-ja and Yu-Geun-Hee with these pharmacological effects can inhibit tyrosinase activity, and these herbal extracts are effective ingredients from plants. It is conventionally used for extracting and prepared by methods well known to those skilled in the art. For example, water, anhydrous or hydrous lower alcohol, acetone, ethyl acetate or diethyl ether having 1 to 3 carbon atoms are added to the medicinal herb powder in an amount of 1 to 15 parts by volume based on the dry weight of the ground product, and then the cooling capacitor Extracted by heating at 50-100 ° C. for 5 to 24 hours in an extractor to which is attached; Water, anhydrous or hydrous lower alcohol, acetone, ethyl acetate or diethyl ether having 1 to 3 carbon atoms are added to the pulverized product in an amount of 1 to 15 parts by volume based on the dry weight of the pulverized product, and then at a temperature of 4 to 25 ° C. Immersion for 3-20 days to obtain an extract; After adding an appropriate amount of water to anhydrous or hydrous ethanol or methanol extract, the resulting precipitate is filtered off, and then added with ethyl acetate, butanol or diethyl ether, mixed well and left to separate the layers, and then the upper layer is separated. The extract is obtained from an extractor with a cooling capacitor. These herbal extracts exhibit not only strong tyrosinase inhibitory activity but also excellent safety on the skin. On the other hand, the extraction filtrate of the herbal medicine extract extracted by the above method is concentrated under reduced pressure, dried, and then blended into the cosmetic.

In order to obtain the desired effect in the present invention, the extract of one or more herbal medicines selected from the group consisting of Samchil-Geun, Radish, Hak-Seul, Gamsong-Hyang, Dae-Geun-Geun, Samnaeja and Yu-Geun-Phi is 0.0001-20% by weight relative to the total weight of the composition, preferably Preferably in an amount of 0.01 to 5% by weight.

On the other hand, the whitening cosmetic composition of the present invention, in addition to the above-described herbal extract, turmeric [Curcuma longa L. and Curcuma aromatica Salisb which is a plant of Zingiberaceae plant known for Root of the root], the water injector (seed of Euphorbia lathyris L.) or a mixture thereof may be further contained within the above-mentioned range.

The whitening cosmetic of the present invention is not particularly limited in the formulation, for example, supple cosmetics, astringent cosmetics, nourishing cosmetics, nutrition cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing foam, cleansing It may have a formulation such as water, pack, powder, body lotion, body cream, body oil, body essence.

In addition, in the whitening cosmetic composition of each formulation, other ingredients in addition to the herbal extracts described above may be appropriately selected and blended by those skilled in the art without difficulty according to the formulation or use purpose of other cosmetics.

Hereinafter, the present invention will be described in more detail with reference to various examples, but the present invention is not limited to these examples.

<Reference Example 1>: Preparation of Herbal Medicine Extract

After grinding 1kg of Samchil-Geun, Radish, Hakseul, Gamsong-Hyang, Dae-Geun-Geun, Samnaeja, Yu-Geun-Pi, Turmeric, and Sucrose, 10 liters of 50% ethanol was added to the ground and then 50-100 in an extractor with a cooling capacitor. After heating at a temperature of 3 hours for 3 hours, the filtrate was filtered under a Whatman No. 2 filter, and concentrated under reduced pressure to obtain a herbal extract with a dry weight of 50 g.

<Test Example 1> Measurement of melanin production inhibitory effect using pigment cells of rat

Mel-Ab cells from C57BL / 6 mice, 10% fetal calf serum, 10 nM 12-O-tetradecanoylphorbol-13-acetate in Dulbecco's modified Eagle's media (DMEM) , 1 nM cholera toxin was added to the culture medium at 37 ° C., 5% CO ₂ . After incubating the cells at a concentration of 10 ⁵ cells / well in a 24-well plate and confirming cell adhesion, the cells were cultured for 3 days by adding a constant concentration of test substance to the cell culture. Here, the test substance is obtained by dissolving the herbal extract of Reference Example 1 in a mixed solvent having a propylene glycol: ethanol: purified water ratio of 5: 3: 2.

After the culture solution was removed, washed with PBS, the cells were dissolved with 1 N sodium hydroxide, and the absorbance was measured at 400 nm. The melanin production inhibition rate was calculated according to Equation 1 below and the results are shown in Table 1.

From Table 1, it is found that each of the herbal extracts of the present invention exhibits a very high melanin production inhibitory effect at a low concentration of about 0.001%, which is superior to that of kojic acid, which is known to exhibit an excellent whitening effect. When the two were mixed together and treated together (the combined concentration of the two herbal extracts was 0.001%).

<Test Example 2> Evaluation of the whitening effect at the animal level

Brown guinea pigs (Tortoiseshell guinea pigs) are animals that cause pigmentation due to ultraviolet rays, PUVA, and allergic reactions, such as humans. If you choose brown, you can use a wide range of areas after hair growth. Pigmentation is produced by the method listed below, and the improvement effect of pigmentation can be screened by the method of apply | coating a whitening agent to the pigmentation area. In addition, the substance whose effects have been recognized by this method can be usefully used as a primary in vivo screening method because of the high probability that the effect also appears in human evaluation in ultraviolet pigmented plaques.

① Preparation of UV Pigment

Pigmentation by ultraviolet light (UVB) is performed by bonding a light shielding aluminum foil having six square windows of 3 × 3 cm to the skin from which the hairs of the brown molot are removed, followed by an SE lamp (wavelength 290 to 320 nm, Toshiba) was irradiated with ultraviolet light (UVB) (total irradiation energy amount was 1350 mJ / cm ² ). After irradiation, the aluminum foil was peeled off and the whitening effect of the test substance was evaluated by the application method as follows. Pigmentation after UV irradiation appears after two or three days, reaching peak after about two weeks. It is important that the resulting color tone of the ultraviolet pigmentation spot is the same and that spots do not form. An individual with poor formation of the ultraviolet pigmentation spot should not be used for good results.

② Coating method of whitening agent

The time point of application of the whitening agent after UV treatment began about 14 days after the pigmentation was established, where the pigmentation was the best. The number of coatings was once or twice a day for 50 days. The coating material was obtained by dissolving the herbal extract of Reference Example 1 at a concentration such that a cumulative stimulus did not appear in a specific solvent (hereinafter referred to as a vehicle in Table 2, a mixed solvent of propylene glycol: ethanol: purified water = 5: 3: 2). A control site was prepared to apply the solvent and to necessarily apply the solvent to other sites. Along with the determination of effects, the cumulative stimulus was also observed.

③ judgment of effect

The effect was determined by measuring the degree of black and white of the skin using a color difference meter (Minolta CR2002). L * a * b * color system is used for displaying a color, and in this invention, L * value (brightness) is mainly used as an index. The L * value is calibrated with a fixed whiteboard, and the measurement is performed evenly on the pigment core by repeating the measurement five times or more at one place. Difference (ΔL *) of the skin color at the start and completion of the application was calculated according to the following equation 1, which is shown in Table 2. The whitening effect is determined by comparing ΔL * between the sample application site and the control site. When △ L * value is about 2, the whitening effect of the deposited pigment is clear, and when it is about 1.5 or more, the whitening effect may be determined. .

From the results of Table 2, it can be seen that the combination of herbal medicines has a whitening effect, and in the case of kojic acid, the red groups of experimental animals are deposited, resulting in a half-effect.

<Test Example 3> Whitening effect test on human skin

After attaching opaque tape with 6 holes of 1.5cm diameter to the upper forearm of the subject, 12 healthy men were irradiated with UVB (1.5-2 times the minimum amount of erythema) of each subject. After inducing, two months after applying the test materials were measured the contrast of the skin using a colorimeter. Each herbal extract was added to the lotion formulation of Table 3 in a concentration of 1%, and the lotion was applied twice a day (morning and evening) daily.

The determination of the effect was determined by obtaining an "L" value representing the skin's contrast as in animal testing (unburned Korean skin color generally shows values between 50 and 70).

If the test substance is effective, the L value is gradually increased, and the comparison between the test substances is expressed as ΔL (final L value-the value immediately before applying the test substance), and the results are shown in Table 4 below. Indicated.

ingredient weight% Beeswax polysorbate 60 sorbitan sesquiolade fluid paraffin squalane caprylic / capric triglyceride glycerin butylene glycol propylene glycol carboxyvinyl polymer triethanolamine antiseptic tablet water herbal extract pigment 4.01.50.55.05.05.03.03.03.00.10.2trace to 1001.0trace

<Test Example 4> Skin irritation effect test

Skin safety was compared using a sample containing 1% of each single herbal ingredient in the formulation used in Test Example 3.

Normal patch test was conducted to check the safety of the skin. After the pinchamber containing the sample was placed on the inner part of the arm of 18 people, after 24 hours and 48 hours, the patch was removed and the skin condition was observed. The average value was calculated and the results are shown in Table 5 below.

(Score)

4: very irritating (skin damage)

3: severe irritation (bubble, etc.)

2: slight irritation (redness)

1: Almost no irritation.

0: No irritation at all, good for sensitive skin.

Sample name Judgment 24 hours later 48 hours later Shamil root 1% formulation 1.2 0.9 Free 1% Formulation 1.3 0.9 1% Formula 1.8 0.7 Hakseul 1% Formulation 1.9 0.8 Sweet persimmon 1% formulation 1.6 0.7 Root Root 1% Formulation 2.0 0.9 Triplex 1% Formulation 1.9 1.1 Turmeric 1% Formulation 0.9 0.5 1% Formulation 1.0 0.5 vehicle 0.2 0.0

From the results of Table 5, it can be seen that each sample has almost no skin irritation, and even if the samples were accumulated directly on the skin for 2 months in Test Example 3, they did not cause any irritation or allergy. It can be evaluated as suitable for use as.

As described above, the whitening cosmetic of the present invention contains one or more herbal extracts selected from the group consisting of Samchil-Geun, Radish, Hak-Seul, Hak-Sul-Hyang, Geun-Shin-Hyang, Dae-Geun-Geun, Samnae-ja, and Root-Skin as whitening ingredients, By further containing a mixture of these, it is possible to provide a whitening cosmetic composition which shows excellent whitening efficacy by improving tyrosinase activity of these herbal extracts and improves skin safety.

Claims (2)

Whitening cosmetic composition, characterized in that it contains one or more herbal extracts selected from the group consisting of Samchil-Geun, Radish, Hak-Seul, Gamsong-Hyang, Dae-Geun-Geun, Samnaeja and Yu-Geun-pi. 2. The whitening cosmetic composition according to claim 1, further comprising an extract of a culprit, turmeric or a mixture thereof.