KR20110043019A - Composition for skin whitening consist of plant exract and cosmetic compostion comprising the plant extract compostion - Google Patents
Composition for skin whitening consist of plant exract and cosmetic compostion comprising the plant extract compostion Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
Abstract
Description
본 발명은 피부 미백 효과가 우수한 천연식물 추출물을 함유하는 조성물 및 이를 포함하여 제조되는 피부 미백 화장료 조성물에 관한 것으로, 더욱 자세하게는 피부 미백 효과를 보이는 감초, 신이, 황금, 포도, 상황의 추출물을 함유하는 식물 추출물 함유 피부 미백 조성물 및 이를 유효성분으로 포함하여 피부 미백 기능을 보이는 화장료 조성물에 관한 것이다.The present invention relates to a composition containing a natural plant extract excellent in skin whitening effect and a skin whitening cosmetic composition prepared by the same, and more particularly contains an extract of licorice, shinyi, golden, grapes, circumstance showing skin whitening effect It relates to a plant extract containing skin whitening composition and a cosmetic composition showing the skin whitening function including the same as an active ingredient.
사람의 피부색을 결정하는데는 피부색소 세포(멜라노사이트 melanocyte)의 활동성, 혈관의 분포, 피부의 두께 및 카로티노이드, 빌리루빈등의 인체 내외의 색소 함유 유무등의 여러요인들이 관여한다. 그 중에서도 가장 중요한 요인은 인체 내의 피부색소세포에서 티로시나제(Tyrosinase)등의 여러 효소가 작용하여 생성되는 멜라닌이라는 흑색색소이다. Determining the skin color of humans involves various factors such as the activity of skin pigment cells (melanosite melanocyte), blood vessel distribution, skin thickness and the presence or absence of pigments in and outside the body such as carotenoids and bilirubin. The most important factor is melanin, which is produced by the action of various enzymes such as tyrosinase on skin pigment cells in the human body.
이 멜라닌 색소의 형성에는 유전적 요인, 호르몬 분비, 스트레스등과 관련된 생리적 요인 및 자외선 조사 등과 같은 환경적 요인등이 영향을 미친다. 멜라닌은 피부색소세포에서 아미노산의 일종인 티로신(tyrosine)이 티로시나제라는 효소에 의해 도파(DOPA)와 도파퀴논(Dopaquinone)으로 전환된 뒤 복잡한 산화 및 축합반응을 거쳐 생성된다. 생성된 멜라닌은 아주 안정한 상태의 고분자 물질로서 색소세포에서 각질화 세포(Keratinocyte)에 전달되고 각질화 세포가 분화되면서 피부 바깥층을 향해 새로 생성되는 각질화 세포에 의해 밀려 올라가다가 최종적으로 표피층에 머무르다가 표피 박리와 함께 상실되어 소멸되는 순환작용을 보인다.Genetic factors, hormonal secretions, physiological factors related to stress, and environmental factors such as UV irradiation affect the formation of the melanin pigment. Melanin is produced by complex oxidation and condensation reactions after the conversion of dopaquinone (DOPA) and dopaquinone (tyrosine), a type of amino acid in skin pigment cells, by an enzyme called tyrosinase. The produced melanin is a very stable polymer material that is transferred from pigment cells to keratinocytes and is differentiated into keratinocytes by the newly produced keratinocytes toward the outer layer of the skin and finally stays in the epidermal layer. It is lost together and disappears.
기미, 주근깨 등의 피부색소침착은 자외선, 호르몬 등의 자극에 의해 피부색소 세포에서 멜라닌이 과다 생성이 일어나서 누적되는 것으로 이를 예방, 치료하기 위해서는 기본적으로 멜라닌 색소의 생성을 조절할 필요가 있다. Skin pigmentation such as blemishes, freckles, etc. is caused by excessive production of melanin in skin pigment cells by stimulation of ultraviolet rays and hormones. In order to prevent and treat this, it is necessary to regulate the production of melanin pigment.
피부 미백 화장품에 있어 멜라닌 생성을 억제하는 방법은 여러 가지가 있다. 첫 번째로 멜라닌 생성을 관여하는 효소인 티로시나제의 활성을 억제하는 등의 등과 같은 피부색소 이상침착증상과 자외선 노출 등에 의해 발생된 과도한 멜라닌 색소 침착을 치료 또는 경감시켜주기 위해서, 이전부터 비타민 C(아스코르빈산), 코지산, 알부틴, 하이드로퀴논, 글루타치온 또는 이들의 유도체, 티로시나제 저해활성을 가진 물질들을 화장료나 의약품에 배합 사용하여 왔는데, 이들은 불충분한 미백 효과, 피부에 대한 안전성문제, 화장료에 배합 시 제형 및 안정성 문제 등으로 인해 그 사용이 제한되고 있다.There are many ways to suppress melanin production in skin whitening cosmetics. First, vitamin C (As) has been previously used to treat or alleviate excessive melanin pigmentation caused by abnormal skin pigmentation and UV exposure, such as inhibiting the activity of tyrosinase, an enzyme involved in melanogenesis. Corbinic acid), kojic acid, arbutin, hydroquinone, glutathione or derivatives thereof, and substances having tyrosinase inhibitory activity have been used in cosmetics and medicines, which have insufficient whitening effects, safety problems for skin, and cosmetics. Its use is limited due to formulation and stability issues.
따라서 본 발명이 해결하고자 하는 기술적 과제는 상술한 문제점이 발생되지 않는 우수한 피부 미백 효능을 가지는 조성물 및 이를 포함하는 화장료 조성물을 제공하는 것이다.Therefore, the technical problem to be solved by the present invention is to provide a composition and a cosmetic composition comprising the same having excellent skin whitening efficacy does not occur the above-described problem.
상기 과제를 달성하기 위하여 본 발명은,In order to achieve the above object, the present invention,
감초 추추물 15 내지 25중량%, 신이 추출물 15 내지 25중량%, 황금 추출물 15 내지 25중량%, 포도 추출물 15 내지 25중량%,상황 추출물 15 내지 25중량%로 이루어진 피부 미백 효능을 가지는 조성물을 제공한다.It provides a composition having a skin whitening effect consisting of 15 to 25% by weight licorice extract, 15 to 25% by weight extract, 15 to 25% by weight golden extract, 15 to 25% by weight grape extract, 15 to 25% by weight situation extract do.
또한 본 발명은,In addition, the present invention,
감초 추추물 15 내지 25중량%, 신이 추출물 15 내지 25중량%, 황금 추출물 15 내지 25중량%, 포도 추출물 15 내지 25중량%,상황 추출물 15 내지 25중량%로 이루어진 피부 미백 효능을 가지는 조성물을 유효성분으로 전체 중량 대비 0.001 내지 10 중량% 포함되는 것을 특징으로 하는 화장료 조성물을 제공한다.A composition having a skin whitening effect comprising 15 to 25% by weight licorice extract, 15 to 25% by weight extract, 15 to 25% by weight golden extract, 15 to 25% by weight grape extract, and 15 to 25% by weight extract It provides a cosmetic composition, characterized in that it comprises 0.001 to 10% by weight relative to the total weight as a component.
본 발명에 다른 피부 미백용 천연식물추출물은 피부 미백과 관련된 티로시나제 활성저해효과, DOPA산화활성저해효과 및 B16F1색소세포를 이용한 멜라닌 생성저해 효과 평가에서 우수한 미백 효과를 나타내었으며, 이 혼합추출물을 함유하는 피부 미백용 화장료 조성물은 피부에 안전할 뿐만 아니라 뛰어나 피부 미백 효과를 가진다.The natural plant extract for skin whitening according to the present invention showed an excellent whitening effect in evaluating tyrosinase activity inhibitory effect, DOPA antioxidant activity inhibitory effect and melanogenesis inhibitory effect using B16F1 pigment cells associated with skin whitening, and containing the mixed extract The cosmetic composition for skin whitening is not only safe for skin but also has an excellent skin whitening effect.
이하 본 발명을 실시하기 위한 구체적인 내용에 대하여 상세하게 설명하기로 한다.Hereinafter will be described in detail with respect to the specific content for practicing the present invention.
본 발명에서의 피부 미백 효능을 가진 추출물을 제조하기 위하여 사용되는 식물은 감초, 신이, 황금, 포도, 상황이다. 김초, 신이, 황금 및 상황은 생약으로 널리 알려져 있는 것으로서 본 발명에서 사용되는 부위 역시 생약으로 사용되는 것과 일치하며, 포도의 경우에는 식용 사용하는 포도 열매를 껍질과 함께 건조하여 사용한다.Plants used to prepare extracts having skin whitening efficacy in the present invention are licorice, sour, golden, grape, circumstance. Gimcho, Sinyi, gold and the situation is widely known as a herbal medicine, the site used in the present invention is also consistent with that used as a herbal medicine, in the case of grapes are used to dry the grape fruit used for edible together with the skin.
또한 감초, 신이, 황금, 포도, 상황의 유효성분 추출 방법은 통상적으로 사용되고 당업자들에게 주지된 방법에 따른다.In addition, licorice, Sinyi, gold, grapes, the method of extracting the active ingredient of the situation is commonly used according to methods well known to those skilled in the art.
예를 들면, 생약재 분쇄물에 물, 탄소수 1~3의 무수 또는 함수 저급알콜, 아세톤, 에틸아세테이트 또는 디에틸에테르를 분쇄물 건조중량애 대하여 1~15 중량배의 양으로 부가한 다음, 냉각콘덴서가 부착된 추출기에서 50~100도씨 온도로 5~24시간 동안 가열하여 추출하거나;상기 분쇄물에 물, 탄소수1~3의 무수 또는 함수 저급알콜,아세톤,에틸아세테이트 또는 디에틸에테르를 분쇄물 건조중량에 대하여 1~15 중량배의 양으로 부가한 다음, 4~25도씨 온도로 3~20일간 침적시켜 추출물을 얻거나; 무수 또는 함수 에탄올 또는 메탄올 추출물에 적당한 양의 물을 가한 뒤 생기는 침전물을 여과하여 제거하고, 여기에 에틸아세테이트, 부탄올 또는 디에틸에테르를 가하여 잘 섞은 다음 방치하여 층을 분리시킨 뒤 상층을 분리한후, 냉각 콘덴서가 부착된 추출기에서 추출물을 얻는다. 이러한 생약재 추출물은 피부에서의 안전성이 우수하다.For example, water, anhydrous or hydrous lower alcohol, acetone, ethyl acetate or diethyl ether having 1 to 3 carbon atoms are added to the medicinal herb powder in an amount of 1 to 15 times by weight based on the dry weight of the ground product, and then the cooling capacitor It is extracted by heating for 5 to 24 hours at an extractor with a temperature of 50 to 100 degrees Celsius; water, anhydrous or hydrous lower alcohol having 1 to 3 carbon atoms, acetone, ethyl acetate or diethyl ether It is added in an amount of 1 to 15 times by weight based on the dry weight, and then immersed at 4 to 25 ℃ temperature for 3 to 20 days to obtain an extract; After adding an appropriate amount of water to anhydrous or hydrous ethanol or methanol extract, the resulting precipitate is filtered off, and then added with ethyl acetate, butanol or diethyl ether, mixed well and left to separate the layers, and then the upper layer is separated. The extract is obtained from an extractor with a cooling condenser. Such herbal extracts have excellent safety on the skin.
한편 상기한 방법에 의해 추출한 식물 추출물의 추출여액을 감압농축하고, 건조한 후, 화장료에 배합한다.Meanwhile, the extract filtrate of the plant extract extracted by the above method is concentrated under reduced pressure, dried, and then blended into a cosmetic.
본 발명에서 피부 미백 효능을 가지는 식물 추출물은 감초 추추물 15 내지 25중량%, 신이 추출물 15 내지 25중량%, 황금 추출물 15 내지 25중량%, 포도 추출물 15 내지 25중량%,상황 추출물 15 내지 25중량%로 이루어지며, 화장료 조성물은 이와 같은 피부 미백 효능을 가지는 식물 추출물 조성물을 전체 중량 대비 0.001 내지 10 중량% 포함되도록 제조한다.Plant extract having a skin whitening effect in the present invention is 15 to 25% by weight licorice extract, 15-25% by weight extract, 15-25% by weight golden extract, 15-25% by weight grape extract, 15-25% by weight extract Consisting of%, the cosmetic composition is prepared to include 0.001 to 10% by weight of the total weight of the plant extract composition having such a skin whitening effect.
상술한 바와 같은 함량의 범위들은 피부 미백 효능을 나타내면서도 화학적 안정성 및 피부 사용시의 안전성이 확보되는 범위를 본 발명자에 의해 다양한 시험을 통하여 결정한 것이다.The range of the content as described above is determined by the present inventors through a variety of tests to show the skin whitening effect while securing the chemical stability and safety in skin use.
이하, 실시예를 통해 본 발명을 좀더 구체적으로 설명한다. 단, 이들 실시예는 본 발명의 예시적인 기재일뿐이며 본 발명의 범위가 이들 실시예에 국한되는 것은 아니다Hereinafter, the present invention will be described in more detail with reference to Examples. However, these examples are merely illustrative of the present invention and the scope of the present invention is not limited to these examples.
제조예 1: 생약재 추출물 제조Preparation Example 1: Preparation of herbal extract
세절하여 음건한 감초, 신이, 황금, 포도, 상황 각각 500g 씩을 혼합하여 95%(V/V) 에탄올 수용액으로 5시간씩 3회 환류 추출하고 냉침한 후, 와트만(Whatman) #5 여과지로 여과하였다. 500 g each of dried licorice, shinyi, golden, grapes, and circumference are shredded, refluxed three times for 5 hours with a 95% (V / V) ethanol solution, cooled, and filtered with Whatman # 5 filter paper. It was.
여과된 추출물은 50℃ 이하에서 감압농축 및 동결건조시켜, 동결건조물로서 각각 10g 정도의 추출물을 수득하였다.The filtered extract was concentrated under reduced pressure and lyophilized at 50 ° C. or lower to obtain an extract of about 10 g each as a lyophilized product.
이 동결건조 추출물을 5 0.001 ~ 30.0중량% 함유되게 정제수, 에탄올, 부틸렌글리콜, 프로필렌글리콜 중에서선택된 1종 이상의 용매를 사용하여 감초, 신이,황금, 포도, 상황 추출물을 제조하였다Licorice, Sinyi, Golden, Grape, Situary extract was prepared using at least one solvent selected from purified water, ethanol, butylene glycol, and propylene glycol to contain 5 0.001 to 30.0% by weight of the lyophilized extract.
시험예 1: Tyrosinase 저해 효과 실험Test Example 1 Tyrosinase Inhibitory Effect Experiment
제조예 1에서 수득한 감초, 신이, 황금, 포도, 상황의 동결건조 추출물 동일 중량을 칭량하여 혼합 후 이를 정세수에 녹여 정제수 내에 추출물 총 함량이 0.001중량%, 0.01중량%, 0.1중량%, 1중량% 및 10중량%가 되도록 하여 멜라닌 합성 주요 단계에 관여하는 티로시나제 효소에 대한 저해력을 측정하여 피부 미백능을 평가하였고 그 결과를 하기 표1에 나타내었다. Weighing the same weight of lyophilized extract of Licorice, Sinyi, Golden, Grapes, Sichuan obtained from Preparation Example 1, mixed and dissolved in purified water, the total content of the extract in purified water was 0.001% by weight, 0.01% by weight, 0.1% by weight, 1% by weight % And 10% by weight to measure the inhibition of the tyrosinase enzyme involved in the main step of melanin synthesis to evaluate the skin whitening ability and the results are shown in Table 1 below.
멜라닌 합성의 주요단계에 기질로 사용되는 티로신 (L-Tyrosine(0.3㎎/㎖) 1.0㎖에 Potassium phosphate buffer solution(pH 6.8, 0.1M) 1.0㎖)과 머쉬룸 티로시나제(Tyrosinase(1250 units/ml) 0.1㎖) 또는 멜라닌 세포로부터 분리한 티로시나제 분획을 첨가하였고 37도씨에서 일정시간 (10분)반응시킨 후 반응생성물인 도파크롬의 흡수파장( 보통 475nm)에서 흡광도를 측정함으로써 원료 및 제품의 피부 미백능력을 평가하였다.Tyrosine (L-Tyrosine (0.3 mg / ml) 1.0 ml in Potassium phosphate buffer solution (pH 6.8, 0.1M)) and mushroom tyrosinase (1250 units / ml) 0.1 Ml) or the tyrosinase fraction isolated from melanocytes was added and reacted at 37 ° C for a certain time (10 minutes), and then measured for absorbance at the absorption wavelength of dopachrome (usually 475 nm). Evaluated.
티로시나제 저해 활성도(%) = 100 - ((ODexp. - ODcon.)/ODstd. X 100)Tyrosinase Inhibition Activity (%) = 100-((ODexp.-ODcon.) / ODstd. X 100)
ODexp.: 샘플실험값, 티로시나제 함유시 측정값ODexp .: Sample test value, measured value with tyrosinase
ODcon.: 샘플실험값, 티로시나제 비함유시 측정값,ODcon .: sample test value, measured value without tyrosinase,
ODstd.: 샘플이 없는 경우, 티로시나제 함유시 측정값ODstd .: measured value with tyrosinase, in the absence of a sample
이때 샘플이 없는 대조군은 식물 추출물 대신 30중량%의 부틸렌글리콜을 넣었다.At this time, the control group without the sample was added 30% by weight of butylene glycol instead of the plant extract.
표 1의 결과를 보면 0.001중량%를 함유 조성물에서도 대조군 보다도 우수한 티로시나제 활성을 저해하는 효능을 나타냈으며 함량이 증가할수록 티로시나제 활성을 저해하는 효능이 증가함을 확인할 수 있었다.The results of Table 1 showed an effect of inhibiting tyrosinase activity superior to the control even in a composition containing 0.001% by weight, it was confirmed that the effect of inhibiting tyrosinase activity increases as the content increases.
시험예 2: 멜라닌 생성억제 효과측정Test Example 2: Measurement of melanin production inhibitory effect
시험예 1에서와 동일하게 식물 추출물 함유 조성물 및 대조군을 제조한 후에 피부 미백 효과를 확인하기 위해 B16F10멜라닌 세포에 대한 멜라닌 합성억제 효과를 측정하였다. In the same manner as in Test Example 1, after preparing a plant extract-containing composition and a control group, melanin synthesis inhibitory effect on B16F10 melanocytes was measured to confirm the skin whitening effect.
B16F10 멜라닌 세포의 멜라닌 합성 억제효과 측정은 다음과 같이 행하였다. B16F10 멜라닌 세포를 6웰 플레이트에 각 웰당 2 x 106 농도로 분주하고 세포를 부착시킨 후 독성을 유발하지 않는 농도로 시료를 처리하여 72시간 동안 배양하였다.The melanin synthesis inhibitory effect of B16F10 melanocytes was measured as follows. B16F10 melanocytes were dispensed in 6-well plates at a concentration of 2 × 10 6 per well, and the cells were attached and incubated for 72 hours by treating the samples at a concentration that does not cause toxicity.
72시간 배양 후 세포를 트립신(trypsin)-EDTA로 떼어낸 후 세포수를 측정한 다음 원심분리하여 세포를 회수하였다. 세포 내 멜라닌의 정량은 로탄(Lotan : Cancer Res., 40: 3345-3350, 1980)의 방법을 약간 변형하여 실시하였다. 셀 펠릿을 PBS로 1회 세척한 후 균질화 완충액(50 mM 소듐 포스페이트, pH 6.8, 1% Triton X-100, 2 mM PMSF) 1㎖를 첨가하여 5분간 와류하여 세포를 파쇄하였다. 원심분리(3,000 rpm, 10분)하여 얻은 세포여액에 1N NaOH(10% DMSO)를 첨가하여 추출된 멜라닌을 용해한 후 마이크로 플레이트 판독기로 405nm에서 멜라닌의 흡광도를 측정한 다음 멜라닌을 정량하여 시료의 멜라닌 생성 저해율(%)을 측정하였다. B16F10 멜라닌 세포의 멜라닌 생성 저해율(%)은 다음과 같이 계산하였다.After 72 hours of incubation, the cells were detached with trypsin-EDTA, the cell number was measured, and the cells were recovered by centrifugation. Quantification of intracellular melanin was performed by slightly modifying the method of Rotan (Cancer Res., 40: 3345-3350, 1980). The cell pellet was washed once with PBS, and then 1 ml of homogenization buffer (50 mM sodium phosphate, pH 6.8, 1% Triton X-100, 2 mM PMSF) was added to vortex for 5 minutes to disrupt the cells. Melanin was extracted by adding 1N NaOH (10% DMSO) to the cell filtrate obtained by centrifugation (3,000 rpm, 10 minutes), and then measured the absorbance of melanin at 405 nm with a microplate reader. Percent inhibition of production was measured. Inhibition rate (%) of B16F10 melanocytes was calculated as follows.
저해율 (%) = [(A-B)/A]x 100% Inhibition = [(A-B) / A] x 100
A : 시료를 첨가하지 않은 웰의 멜라닌 양A: Melanin amount in wells without sample
B : 시료를 첨가한 웰의 멜라닌 양B: Melanin amount in the wells to which the sample was added
표 2의 결과를 보면 0.001중량%를 함유 조성물에서도 대조군보다도 우수한 멜라닌의 생성을 저해하는 효능을 나타냈으며 함량이 증가할수록 티로시나제 활성을 저해 등의 방법으로 멜라닌 생성을 저해하는 효능이 증가함을 확인할 수 있었다. 하지만 10중량%에서는 농도가 너무 높아서 세포배양액상에서 세포의 정상적인 대사활동에 영향을 미치는 것으로 오히려 멜라닌 생성 저해효과가 줄어들었지만 세포의 정상적인 대사활동에 영향을 미치지 않는 범위 내에서는 시료의 멜라닌 생성저해효과는 농도 의존적으로 증가되었음을 확인할 수 있었다. The results of Table 2 showed that the composition containing 0.001% by weight inhibited the production of melanin better than the control group, and as the content increased, the effect of inhibiting melanin production was increased by inhibiting tyrosinase activity. there was. However, at 10% by weight, the concentration was too high to affect the normal metabolic activity of the cells in the cell culture liquid, but the melanin production inhibitory effect was reduced, but the melanin inhibitory effect of the sample was within the range that does not affect the normal metabolic activity of the cells. It was confirmed that the concentration increased.
시험예 3: 인체 피부에 대한 피부 미백 효과 시험Test Example 3: Skin whitening effect test on human skin
제조예 1에서 수득한 감초, 신이, 황금, 포도, 상황의 동결건조 추출물 동일 중량을 칭량하여 혼합 후 이를 종래의 로션에 함유시켜 추출물 총 함량이 0.001중량%, 0.01중량%, 0.1중량%, 1중량% 및 10중량%가 되도록 로션을 제조하였으며, 동일한 로션이지만 본 발명에 따른 식물 추출 조성물을 전혀 포함하지 않은 로션을 대조군을 하여 인체피부에 대한 피부 미백 효과 시험을 실시하여 그 결과를 표 4 나타냈다.Licorice, Sinyi, golden, grapes, lyophilized extract of the situation obtained in Preparation Example 1 was weighed and mixed and then contained in a conventional lotion, the total content of the extract is 0.001% by weight, 0.01% by weight, 0.1% by weight, 1 The lotion was prepared to be 10% by weight and 10% by weight, and the same lotion but no lotion containing the plant extract composition according to the present invention was subjected to the skin whitening effect test on human skin using the control group, and the results are shown in Table 4 below. .
상술 로션의 처방은 아래의 표 3과 같았다.The prescription of the lotion was shown in Table 3 below.
인체 피부에 대한 피부 미백 효과 시험은 건강한 20명의 여성을 대상으로 피검자의 상박부 내측에 직경 1.5cm의 구멍 6개가 뚫린 불투명 테이프를 부착한 뒤, 각 피검자의 최소홍반량의 1.5~2배 정도의 자외선(UVB)을조사하여 피부의 흑화를 유도하였다. 상기에서 제조된 본 발명에 따른 로션과 대조군을 하루에 2회씩 매일 도포한 후, 두 달 후에 크로마메터(Minolta CR300)를 이용하여 피부의 명암을 측정하였다. 효과의 판정은 피부의 명암을 나타내는 "L"값을 구하여 결정하였다(태우지 않은 한국인피부색은 일반적으로 50~70의 값을 나타냄). 시험물질을 도포하여 효과가 있는 경우는 L값이 점차 증가하게 되며, 시험물질들 사이의 비교는 색의 밝기변화(ΔL)값(최종 L값-시험물질 도포전의 값)으로 표현하였다. ΔL값이 클수록 피부 미백 효과가 큰 것이다.The skin whitening effect test on human skin was performed on 20 healthy women by attaching an opaque tape with 6 holes of 1.5cm diameter inside the upper arm of the subject, and measuring 1.5 ~ 2 times the minimum erythema of each subject. Ultraviolet rays (UVB) were irradiated to induce skin blackening. After applying the lotion and the control group according to the present invention prepared twice daily, two days later, the contrast of the skin was measured using a chromameter (Minolta CR300) two months later. The determination of the effect was determined by obtaining a "L" value representing the contrast of the skin (unburned Korean skin color generally indicates a value of 50-70). When the test material is applied, the L value gradually increases, and the comparison between the test materials is expressed as the change in brightness (ΔL) value of the color (final L value-the value before application of the test material). The larger the ΔL value, the greater the skin whitening effect.
표 4의 결과를 보면 0.001중량%를 함유 조성물에서도 대조군보다도 우수한 피부 미백 효과를 보임을 알 수 있으며 또한 함량의 증가에 따라 피부 미백 효과가 증감함을 알 수 있습니다.The results of Table 4 show that the skin whitening effect is superior to the control even in the composition containing 0.001% by weight, and it can be seen that the skin whitening effect increases and decreases as the content is increased.
시험예 5: 피부자극 효과시험Test Example 5: Skin irritation effect test
시험예 4에서 제조한 로션을 이용하여 피부 자극성을 시험하기 위해, 건강한 성인 남녀 20명을 대상으로 패취테스트를 실시하였다. 팔 아래의 안쪽부위에 시료를 담은 핀쳄버(Finn chamber, 100× 10, EPITEST, 핀란드)를 첩포한 후 24시간 후와 48시간 후에 첩포를 제거하고 확대경(8MC-150, DAZOR, 미국)을 이용 피부 상태를 관찰하여 국제접촉피부염연구회의 규정에 따라 판정하여 피검자 20명의 평균값을 계산하였고 그 결과는 하기 표 5에 나타내었다.In order to test skin irritation using the lotion prepared in Test Example 4, a patch test was conducted on 20 healthy adults and men. After applying the pin chamber (Finn chamber, 100 × 10, EPITEST, Finland) containing the sample in the inner part of the arm, remove the patch after 24 hours and 48 hours and use a magnifying glass (8MC-150, DAZOR, USA). The skin condition was observed and determined according to the regulations of the International Contact Dermatitis Research Association. The average value of 20 subjects was calculated and the results are shown in Table 5 below.
(피부 반응의 평가기준 및 점수)(Evaluation Criteria and Score of Skin Response)
6 : 대수포 형성6: cannon formation
3 : 심한 홍반 및 소포형성3: severe erythema and vesicle formation
2 : 뚜렷한 홍반, 구진 및 소수포2: pronounced erythema, papules and vesicles
1 : 경계가 뚜렷하나 약한 홍반, 부종 및 구진1: borderline but weak erythema, edema and papules
0.5 : 희미한 또는 가벼운 홍반0.5: faint or light erythema
0 : 무반응, 정상피부0: no response, normal skin
Sample name
표 5에서 보는 바와 같이 대조군과 본 발명에 따른 조성물 모두 부반응의 정상 피부를 보여 피부 자극이 없음을 확인할 수 있었습니다. As shown in Table 5, both the control group and the composition according to the present invention showed normal skin of side reactions, indicating no skin irritation.
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