KR20070079390A - Cosmetic composition for skin whitening - Google Patents

Abstract

A cosmetic composition for skin whitening is provided to improve or prevent abnormal symptom of skin pigmentation and excessive skin pigmentation by inhibiting melanogenesis of the skin and inhibiting and improving the skin pigmentation such as chloasma, freckle, and dullness. The skin whitening cosmetic composition comprises 0.001-20 wt.% of co-enzyme Q10, 0.001-20 wt.% of beta-carotene, 0.001-20 wt.% of tocopherol, 0.001-20 wt.% of an extract of Malva Sylvestris and 0.001-20 wt.% of an extract of Primula Veris.

Description

Cosmetic composition for skin whitening {Cosmetic composition for skin whitening}

The present invention relates to a cosmetic composition for skin whitening that improves or prevents skin pigment abnormalities and skin pigment overdeposition. More specifically, by containing coenzyme Q10 (ubiquinone), beta-carotene, tocopherol, mellow extract and Primula extract to inhibit melanin production of the skin, such as blemishes, freckles, skin blemishes and sunburn skin The present invention relates to a cosmetic composition for skin whitening having an effect of inhibiting and improving skin pigmentation.

Melanin (melanin), which determines the color of human skin, is produced in melanocytes, which work with enzymes such as tyrosinase, which is present in melanocytes, to form tyrosine, an amino acid that is always present in vivo. The polymerization oxidation reaction is carried out to form melanin, which is a dark brown pigment. The melanin thus formed is transferred to the epidermal cells called keratinocytes through the dendritic processes of melanocytes, and there is no enzyme that degrades melanin in the body, but it is separated from the skin as keratinocytes are separated from the epidermis. Is removed.

The melanin thus formed forms a cap-like structure around the nucleus, and plays an important role such as protecting genes from ultraviolet rays and removing free radicals to protect intracellular proteins. However, if the melanin is produced more than necessary, it causes the hyperpigmentation, such as blemishes, freckles, spots, etc., resulting in cosmetically bad results. In addition, as more people enjoy working outside due to the increase in the leisure population, melanin pigmentation due to ultraviolet rays is intensifying, and hyperpigmentation is a serious mental burden in terms of skin beauty, which can interfere with normal social activities. In particular, women in the Asian region have long favored white and fine skin, and this has become an important criterion of beauty, and the desire for prevention and improvement of abnormal skin pigmentation and hyperpigmentation is increasing, thus preventing overproduction of melanin. The development of cosmetics and drugs for whitening for the purpose is actively progressing.

For example, conventionally, substances having tyrosinase inhibitory activities such as ascorbic acid, hydroquinone, hutaquinone, glutathione, arbutin, and the like have been used in cosmetics or pharmaceuticals, but among them, Most of them are ineffective or unstable in formulation. Hydroquinone-like compounds show strong depigmentation, but they have skin sensitization, which can cause skin allergies and change the function of normal skin. Its use is limited in terms of safety to the skin.

Accordingly, the present inventors conducted a study for the development of cosmetics having excellent skin whitening effect and no instability, skin irritation, sensitization, mutagenicity, etc. in the formulation, as a representative extract of mellow extract and coenzyme Q10, beta-carotene and tocopherol By further containing an active ingredient of Primula extract, a cosmetic composition with excellent whitening effect of improving or preventing skin pigment abnormalities and skin pigment overdeposition is completed and the present invention has been completed.

Accordingly, it is an object of the present invention to provide a cosmetic composition for skin whitening containing coenzyme Q10, beta-carotene, tocopherol, mellow extract and primula extract.

In order to achieve the above object, the cosmetic composition for skin whitening of the present invention contains coenzyme Q10, beta-carotene, tocopherol, mellow (mallow, genus: Malva Sylvestris ) extract and Primula (primrose, genus: Primula Veris ) extract Characterized in that.

In addition, the content of the coenzyme Q10, beta-carotene, tocopherol, mellow extract and Primula extract is characterized in that each of 0.001 ~ 20% by weight relative to the total weight of the composition.

Hereinafter, the present invention will be described in more detail.

Coenzyme Q10 used in the present invention is also called ubiquinone, ubidecarenone, and the like and is actually present in human cells to act as a major coenzyme of energy metabolism. In addition, Coenzyme Q10 is a powerful antioxidant that prevents skin damage, skin cancer, photoaging or skin pigmentation caused by ultraviolet rays mediated by reactive oxygen species.It is used to inhibit skin pigmentation and hyperpigmentation by inhibiting melanin formation. It has the effect of improving deposition. The preferred content of such coenzyme Q10 is 0.001 to 20% by weight based on the total weight of the composition, because less than 0.001% by weight of the whitening effect on the skin is weak, and more than 20% by weight is not suitable for cosmetics, such as formulation stabilization. to be.

In addition, beta carotene and tocopherol used in the present invention is a representative vitamin component beta carotene is a kind of carotenoids, a pigment contained in a lot of green and yellow vegetables, and converted into vitamin A in the body has the properties of a precursor of vitamin A. Beta carotene has a strong antioxidant action, protects normal cells from harmful oxygen free radicals and activates skin cells. Beta-carotene, which has a regenerative effect, does not play a large role in itself, but it has the ability to help metabolize vitamin A in the body. In addition, it has the function of healing the skin damaged by UV rays and pollution, and also has the function of protecting the skin and whitening function by actively helping skin cells subjected to strong summer sun.

In addition, the tocopherol is a powerful antioxidant called vitamin E, and has been widely used in cosmetics and food for a long time. Studies on the Melanin Synthesis-Assisting Function of Antioxidant Vitamins According to the study, β-type tocopherol and r-type tocopherol have relatively strong melanin synthesis due to the direct detoxification effect of intracellular chirosinase activity and the detoxification activity at the transcription level of tyrosinase and TRP-2. It was found to have deliquescent activity. In addition, δ-type tocopherol has been found to have tyrosinase activator, and the mechanism of TRP-2 mRNA synthesis has been reported, and it has been reported that there is no effect on tyrosinase mRNA synthesis.

The content of the antioxidant beta carotene and tocopherol is preferably 0.001 to 20% by weight based on the total weight of the composition. If the content of each component is less than 0.001% by weight, it is difficult to expect a whitening effect due to the slight effect on the skin, and when it is more than 20% by weight, it is not suitable as a cosmetic because it may cause discoloration and odor, as well as skin irritation. .

Mellow (mallow, genus: Malva Sylvestris ) used in the present invention is a herb that is old enough to be used for various purposes since ancient times. In ancient Arabia, it was used to eliminate inflammation. Primula Veris is a herbaceous herb that is a perennial herbaceous plant and has been used for many years in the European community. It is used in Jinhae, expectorant, relieving and bronchitis in Korea and contains a large amount of saponin.

Mellow also contains compounds of hydrolyzable mucus arabinose, glucose, rhamnose, galactose galacturonic acid and tannins, and is widely used as a respiratory therapeutic agent. This plant contains 5-10% saponin, phenolic glucoside and primulaverin and contains a small amount of sugar and tannin.

In the present invention, the preferred content of the mellow extract and Primula extract used for whitening is 0.001 to 20% by weight based on the total weight of the composition, and when the content of each component is less than 0.001% by weight, the effect on the skin is weak, If it is more than 20% by weight, it is not suitable as a cosmetic for reasons such as discoloration and deodorization as well as occurrence of precipitation in the formulation.

Mellow extract of the present invention is a component extracted from the leaves, stems, roots of Malva Sylvestris .

In addition, Primula of the present invention is a component extracted from the leaves, stems, roots of Primula Veris . Mellow and Primula extracts can be obtained by extracting using conventional plant extraction methods such as distillation, solvent extraction, and the like.

The cosmetic composition of the present invention is not particularly limited in its formulation, and may be, for example, a cosmetic composition having a formulation of a flexible cosmetic water, nourishing cosmetic water, massage cream, nutrition cream, essence, pack, gel or skin adhesive type cosmetic. .

Hereinafter, although an Example and a test example are given and this invention is demonstrated more concretely, the scope of the present invention is not necessarily limited only to these examples.

Test Example 1: Measurement of melanin production inhibitory effect

Minimum HM3KO cells (Y. Funasaka, Department of dermatology, Kobe university school of medicine, 5-1 Kusunoki-cho 7-chrome, Chuo-ku, Kobe 650, Japan), human melanoma cells Incubated in Essential Medium (MEM) at 37 ℃, 5% CO ₂ conditions. The cultured cells were spread in 75 flasks so that the number of cells was 3 × 10 ⁵ per flask, and the cells were allowed to adhere to the wall for one night. After confirming that the cells adhered well, the medium was prepared with the composition of Table 1 below. The fresh medium containing 10 ppm of the test substance of complex I, II and III was changed. As a control, a medium containing DMSO was used. In this manner, the cells were incubated with a new medium containing the sample every two to three days, until the cells were filled in the flask. When the cells are grown, the cells of the control and the control groups are compared and the results are shown in Table 1 below. In addition, the culture medium was removed, washed with PBS, dissolved with 1N sodium hydroxide, and measured for absorbance at 500 nm, and then the melanin production inhibition rate was calculated according to Equation 1 shown in Table 1 below.

Melanin inhibition rate (%) = 100-(absorbance of each test substance / absorbance of control × 100)

ingredient Melanin production inhibition rate (%) Coenzyme Q10 23.2 Beta carotene 13.7 Tocopherol 12.1 Mellow Extract 9.8 Primula Extract 11.5 WQP complex I (coenzyme Q10: beta carotene: tocopherol = 1: 1: 1) 29.3 WQP complex Ⅱ (Coenzyme Q10: Mellow extract: Primula extract = 1: 1: 1) 26.7 WQP complex III (Coenzyme Q10: Beta-carotene: Tocopherol: Mellow extract: Primula extract = 1: 1: 1: 1: 1) 32.5

* Coenzyme Q10 (NISSHIN PHARMA, Japan)

** Beta-carotene (Colletica, France)

*** Tocophenol (Colletica, France)

**** Mellow Extract (Pentapharm, Switzerland)

***** Primula Extract (Pentapharm, Switzerland)

From the results of Table 1, the whitening effect of the WQP complexes I and II was superior to that of each sample individually, and the best whitening of the WQP complex III, that is, the whitening active ingredient, was used together. The effect was concluded.

 [Test Example 2: Evaluation of the whitening effect at the animal level]

Brown guinea pigs (Tortoiseshell guinea pigs) are animals that cause pigmentation due to ultraviolet rays, PUVA, and allergic reactions, such as humans.As the color of the hair is the same, the color of the hair is uniform and brown. After removal, a wide range of sites can be used. Pigmentation was produced by the method listed below, and the improvement effect of pigmentation was verified by the method of apply | coating the whitening composition to the pigmentation site | part. In addition, the substances whose effects have been recognized in this way are useful as primary in vivo screening methods because they have a high probability of being effective in evaluation in human UV pigment plaques.

① Preparation of UV Pigment

Pigmentation by ultraviolet (UVB) was carried out by bonding a light-shielding aluminum foil having six square windows of 3 × 3 cm to the skin from which the hairs behind the brown malmot were removed, followed by an SE lamp (wavelength 290-320 nm, Toshiba). Ultraviolet (UVB) was irradiated with (the total irradiation energy amount was 1350mJ / cm ² ). After irradiation, the aluminum foil was peeled off and the whitening effect of the test substance was evaluated by the following coating method. Pigmentation after UV irradiation appears 2-3 days later, reaching peak after about 2 weeks. At this time, it is important that the generated color tone of the ultraviolet dye spot is the same and does not cause staining, and an object having poor formation of the ultraviolet dye spot is not used for good results.

② Application of Composition

After ultraviolet irradiation, the composition of Table 2 began to be applied about 14 days after the pigmentation was the highest, that is, the pigmentation was formed. The application number of times was continued for 50 days once or twice a day. The composition was dissolved and diluted in a mixed solvent of propylene glycol: ethanol: water = 5: 3: 2 and applied with a cotton swab to prepare a control site for necessarily applying a solvent to other sites. Along with the determination of effects, the cumulative stimulus was also observed.

 ③ judgment of effect

The effect was determined by measuring the degree of black and white of the skin using a color difference meter (Minolta CR2002). L * a * b * colorimeter is used for displaying a color, and in this invention, L * value (brightness) was mainly used as an index. The L * value was calibrated with a standard whiteboard, and the pigmentation part was measured evenly by repeating the measurement five times or more at one site. The difference in skin color (ΔL *) at the start and completion of the application was calculated according to Equation 2 below, which is shown in Table 3. The whitening effect was determined by comparing ΔL * between the sample application site and the control site. When the ΔL * value was about 2, the whitening effect of the deposited pigment was apparent, and when it was about 1.5 or more, the whitening effect was determined.

△ L * = L * value 50 days after application-L * value at the start of application

Whitening Composition (WQP Complex III) ingredient content % Coenzyme Q10 10 Beta carotene 10 Tocopherol 10 Mellow Extract 10 Primula Extract 10

Melanin production inhibitory effect Material Concentration (%) Whitening Effect (△ L) Whitening Composition 1.0% 2.72 Whitening composition 0.5% 1.72 Whitening composition 0.1% 1.52 Kojisan 1.0% 0.75 Control 0.78

From the results of Table 3, the composition of the present invention can be seen that the whitening of the pigment deposited at a concentration of 1.0% is clear and exhibits a whitening effect even at a concentration of 0.1%. It was found that the deposition resulted in the effect being halved.

[Examples 1-3 and Comparative Examples 1-6]

In order to test the whitening effect on the human skin, raw materials 1-6 and 10,11 shown in Table 4 were mixed, dissolved at 70 ° C., and used as water parts. In addition, raw materials 7-9 and 12-19 were melt | dissolved at 70 degreeC, and it was set as the oil part. The oil part was added to the water part, stirred with a homomixer (Tokushu Kika Co., Ltd., Japan), and first emulsified. After removing bubbles, the mixture was cooled to room temperature and used in Examples 1-3 and Comparative Examples 1-6.

ingredient Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 1. Purified water To100 To100 To100 To100 To100 To100 To100 To 100 To 100 2. Glycerin 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 3. Butylene Glycol 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 4. Beta Glucan 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 4.0 5. Hyaluronic Acid Extract 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 6. Carbomer 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 7. Coenzyme Q10 0.5 0.5 0.5 0.5 - - - - - 8. Beta Carotene 0.5 0.5 - - 0.5 - - - - 9. Tocopherol 0.5 0.5 - - - 0.5 - - - 10. Mellow Extract 0.5 - 0.5 - - - 0.5 - - 11. Primula Extract 0.5 - 0.5 - - - - 0.5 - 12. Kojisan - - - - - - - - 1.0 13. Caprylic Capric Triglycerides 8.0 8.0 8.0 8.0 8.0 8.0 8.0 8.0 8.0 14. Hydrogenated polydecene 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 15. Cetearyl Glucoside 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 16. Sorbitan Stearate 0.4 0.4 0.4 0.4 0.4 0.4 0.4 0.4 0.4 17. Cetearyl Alcohol 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 18. Preservative Quantity Quantity Quantity Quantity Quantity Quantity Quantity Quantity Quantity 19. Incense Quantity Quantity Quantity Quantity Quantity Quantity Quantity Quantity Quantity 20. Triethanol Amine 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1

[Test Example 3: Whitening effect test on the human skin]

Twelve healthy men were attached with an opaque tape with six 1.5cm diameter holes in the upper forearm of the subject, and 1.5 ~ 2 times the UVB of the minimum Erythema Dose of each subject. The blackening of the skin was induced by irradiating and the test materials were applied and two months later, the contrast of the skin was measured using a color difference meter. Each test substance was applied to the skin external preparations of Examples 1 to 3 and Comparative Examples 1 to 6 twice daily in the morning and evening.

Judgment of the effect was determined by obtaining an "L" value representing the skin's contrast as in animal testing (unburned Korean skin color generally ranges from 50-70).

When the test substance is effective, the L value is gradually increased, and the comparison between the test substances is calculated according to the following equation (2) of the difference in skin color (ΔL) between the start point and the end point of the application. Table 5 shows.

Whitening effect on human skin Test substance Whitening Effect (△ L) Example 1 3.86 Example 2 2.88 Example 3 2.24 Comparative Example 1 1.92 Comparative Example 2 1.75 Comparative Example 3 1.84 Comparative Example 4 1.55 Comparative Example 5 1.77 Comparative Example 6 2.01 Control 1.27

As can be seen in Table 5 above, coenzyme Q10, beta-carotene, tocopherol and coenzyme Q10, beta-carotene, tocopherol extract and coenzyme Q10, beta-carotene, tocopherol, and coenzyme Q10, mellow extract and free The whitening effect was found to be higher in the same composition as the Mulla extract, and all of the five whitening agents, coenzyme Q10, beta-carotene, tocopherol, mellow extract and Primula extract, were used together. It showed the best effect on the whitening effect. Based on the results, it was found that the above raw materials have a synergistic effect in the whitening composition.

As described above, the cosmetic composition provided by the present invention is safe to the skin and is stable in the formulation by using coenzyme Q10, beta-carotene and tocopherol and the herbal plant extract mellow extract and pramula extract, which are the target antioxidants And it can be used as a cosmetic composition for whitening excellent whitening effect.

Claims (2)

A cosmetic composition for skin whitening characterized by containing coenzyme Q10, beta-carotene, tocopherol, mellow (mallow sylvia, Malva Sylvestris ) extract and primula (primrose, Primula Veris ) extract. The cosmetic composition for skin whitening according to claim 1, wherein each of the coenzyme Q10, beta-carotene, tocopherol, mellow extract and pramula extract is 0.001 to 20% by weight based on the total weight of the composition.