KR100959393B1 - Skin whitening cosmetics composition containing an effective ingredient extracted from Acanthopanax sessiliflorum Seeman and manufacturing method thereof - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin whitening, characterized in that it comprises a chlorogenic acid fraction extracted from agar. The cosmetic composition according to the present invention provides excellent whitening effect by inhibiting melanin pigment production and inhibiting pigmentation of the skin without side effects. The present invention also provides a method for increasing the skin whitening effect of the extract of Ogapi.

Ogapi, Chloroic Acid, Skin Whitening

Description

Skin whitening cosmetics composition containing an effective ingredient extracted from Acanthopanax sessiliflorum Seeman and manufacturing method

The present invention relates to a cosmetic composition for skin whitening containing a plant extract or an active ingredient extracted from a plant and a method for producing such a cosmetic composition.

Ogapi, Acanthopanacis Cortex, Acanthopanax sessiliflorum Seeman is a deciduous broad-leaved shrub belonging to the genus Acanthopanax belonging to the family Araliaceae. Ogapi is a herbal medicine that was collected from the Korean Pharmacopoeia and defined as 'the bark of the roots, stems and branches of Acanthopanax sessiliflorum Seeman or related plants'.

These scabies are highly valued as medicinal and many studies have been conducted on the component analysis of scabies. The usefulness of acanthoic acid and acanthoside AG, sigmasterol, chlorogenic acid, caffeic acid, sesamin, chisanoside, etc. It has been reported that various physiological effects such as increased body function, central nervous system excitability, muscle exercise, anticancer action, antidiabetic action, sleep extension, detoxification action and adaptation to environmental changes are included. Because of this efficacy, it is used as an additive in food, medicine, cosmetics, etc., and research on the component analysis and efficacy of the organ has been ongoing.

Until now, researches have been conducted on the pharmacological and physiological effects of Acanthopanax plants and their separation and purification from the main bioactive substances. Acanthopanax Senticosus) and mixed odorless garlic extract showed inhibition of skin aging (Republic of Korea Patent Publication 10-2000-0052725), gasiohgapi extract (Acanthopanax Senticosus ) treatment effect of acne and rashes (Korean Patent Laid-Open Publication No. 10-2000-0009820) and Ogapi extract ( Acanthopanax) sessiliflorum Seeman) has been reported to be excellent skin protection effect (Korean Patent Publication No. 10-1999-0053765).

On the other hand, melanin is a natural pigment of a polymer widely present in animal and plant systems, and in human skin, biosynthesis is promoted by a mechanism against skin damage caused by ultraviolet irradiation. Melanin pigments have a positive aspect of protecting the skin, but their overproduction causes blemishes, freckles and skin spots, and also promotes cell death and skin cancer production due to the toxicity of melanin precursors.

The biosynthesis of melanin is characterized by specific enzymes such as tyrosinase, tyrosinase related protein-1 (TRP-1), and dopachrome tautomerase (DCT) in the melanosomes of melanocytes in the epidermal basal layer. It is caused by a continuous oxidation reaction, of which the tyrosinase activity inhibition test, a major enzyme of melanin synthesis, is being adopted in the early stage of the development of melanin inhibitor.

However, in addition to oxidative reactions caused by tyrosinase, various complex factors, including melanocyte stimulating hormone (MSH), various cytokines involved in inflammatory reactions, and gene expression factors, have been found to affect melanogenesis. In addition, it has been focused on the development of a substance that inhibits the production of melanin comprehensively due to the problem that tyrosinase activity inhibitor in vitro does not show any activity in melanoma cell line.

To date, tyrosinase activity inhibitors such as ultraviolet absorbers, scattering agents, arbutin, kojic acid, hydroquinone, ascorbic acid and derivatives that eliminate reactive oxygen species, and coenzyme Although Q10 (coenzyme Q10) is known to have a whitening effect, it is not used due to problems such as skin safety and formulation stability or only a limited amount is used.

Therefore, the technical problem to be achieved by the present invention is to provide a cosmetic composition and a method for producing the composition having excellent skin whitening effect by inhibiting the production of melanin while having less skin side effects.

In order to achieve the above technical problem, the present invention provides a cosmetic composition for skin whitening, characterized in that it contains an extract fraction rich in chlorogenic acid extracted from the organ. In the present invention, Ogapi is called 'Ogapi Acanthopanax sessiliflorum Seeman or bark of roots, stems and branches of the same plant.

The present invention also comprises the steps of (S1) crushing Ogapi; (S2) a step of extracting room temperature or cold extraction with 70-85% ethanol aqueous solution; (S3) extracting the filtrate under reduced pressure at 20 to 50 ° C. to obtain a crude extract; (S4) filling the crude extract with ion resin column chromatography and sequentially eluting with an ethanol aqueous solution while increasing the content of ethanol; And (S5) recovering a 10 to 40% ethanol aqueous solution fraction to adjust the content of chlorogenic acid, thereby providing a method for producing an ogapi extract with improved whitening effect.

Hereinafter, a cosmetic composition for skin whitening comprising the extract of Ogapi according to the present invention and a method for preparing the composition will be described in more detail.

The cosmetic composition for skin whitening of the present invention comprises a chlorogenic acid-rich fraction extracted from agar skin as the skin whitening active ingredient.

The present invention is based on the surprising fact that the skin whitening effect increases as the chlorogenic acid represented by the following formula (1) in the extract of Ogapi and the content of such chlorogenic acid can be increased through a specific manufacturing method.

In the cosmetic composition for skin whitening of the present invention, the content of the chlorogenic acid is preferably 0.001 to 10% by weight based on the total weight of the cosmetic composition. If the content of the active ingredient is less than 0.001% by weight can not achieve a sufficient skin whitening effect of the original desired skin is not preferable, if the content exceeds 10% by weight may cause problems in the stability of the cosmetic And, due to this there is a disadvantage that can not express a sufficient cosmetic effect when using the cosmetics or appearance.

Ogapi extract according to the present invention can be obtained using a variety of organs, tissues (eg, roots, stems, fruits, flowers, leaves) and the like of the agapi, preferably an extract obtained from the stems and roots of the agapi.

Typical extracts of Ogapi include various extracting solvents such as water, anhydrous or hydrous lower alcohols having 1 to 4 carbon atoms (methanol, ethanol, propanol, butanol, etc.), mixed solvents of the lower alcohols with water, acetone, ethyl acetate , Chloroform, 1,3-butylene glycol, butyl acetate and the like can be obtained as an extraction solvent, but in order to obtain an excellent skin whitening effect, using an aqueous solution of 70 to 85% alcohol 10 to 100 ℃, preferably The extraction is preferably performed at 15 to 40 ° C. for about 2 hours to 5 days, and more preferably using 75% aqueous ethanol solution.

Extraction can be obtained by extraction methods such as room temperature extraction, ultrasonic extraction, reflux cooling extraction, etc.However, in order to obtain the extract of Ogapi with excellent skin whitening effect, the extract is filtered several times with a room temperature extraction method, and the extract is filtered and concentrated under reduced pressure at 40 ° C. The procedure for obtaining the extract is preferred. When the extraction temperature exceeds 50 ° C. in the preparation of Ogapi crude extract, the amount of the total extract is increased, but the content of chlorogenic acid does not increase, and the color of the crude extract is darkened, so it is preferable to prepare at 10 to 50 ° C.

The present invention also comprises the steps of (S1) crushing Ogapi; (S2) a step of extracting room temperature or cold extraction with 70-85% ethanol aqueous solution; (S3) extracting the filtrate under reduced pressure at 20 to 50 ° C. to obtain a crude extract; (S4) filling the crude extract with ion resin column chromatography and sequentially eluting with an ethanol aqueous solution while increasing the content of ethanol; And (S5) recovering a 10 to 40% ethanol aqueous solution fraction to adjust the content of chlorogenic acid, thereby providing a method for producing an ogapi extract with improved whitening effect.

The cosmetic composition according to the present invention may be any one formulation selected from the group consisting of cosmetics, essences, skins, lotions, creams, packs, foundations, and makeup bases, and also contains the Ogapi extract-containing cosmetic composition Silver may be used for the production of basic cosmetics or color cosmetics. In addition, the cosmetic composition of the present invention can be applied in various forms such as liquid, cream, paste, solid, and can be prepared using a conventional cosmetic preparation method.

As described above, the present invention provides a cosmetic composition for skin whitening comprising a chlorogenic acid-rich fraction extracted from agar skin as an active ingredient, and also a method for producing a cosmetic composition excellent in such a skin whitening effect, that is, the It provides a way to increase the skin whitening effect. Ogapi extract according to the present invention as a natural product has no skin side effects, as well as safe, it can be useful as a cosmetic composition for skin whitening because it exhibits excellent activity in the antioxidant effect and inhibiting melanogenesis in cells using B16F1 melanocytes.

Hereinafter, the present invention will be described in detail with reference to Examples. However, embodiments according to the present invention can be modified in many different forms, the scope of the present invention should not be construed as limited to the embodiments described below. The embodiments of the present invention are provided to more completely explain the present invention to those skilled in the art.

<Extraction Examples 1 to 5>

50 g of stems of stems were quantified and placed in an extractor. Then, 1 L of each extraction solvent as shown in Table 1 was added and reflux-cooled, followed by filtering with a filter sheet of No. 2 twice. The extract filtrate was concentrated under reduced pressure at 60 ° C. with a rotary evaporator to show the yield of the extract of Ogapi and the content (%) of chlorogenic acid as an active ingredient, as shown in Table 1. At this time, the content was measured by high performance liquid chromatography, and the analysis conditions are as follows.

Chromatography: Agilent Technologies 1200 Series

Column: Luna C18 (5 um, 250 × 4.6 mm)

Detector: Ultraviolet absorption spectrophotometer (wavelength 330 nm)

Flow rate: 1.0 ml / min

Mobile phase: gradient solvent of solvent A (0.1% acetic acid, acetonitrile), solvent B (0.1% acetic acid, water)

Extraction Example Extraction solvent Yield (g) Chlorogenic Acid Content (%) One 100% ethanol aqueous solution 0.950 0.994 2 75% ethanol aqueous solution 2.920 2.266 3 50% ethanol aqueous solution 3.500 2.117 4 25% ethanol aqueous solution 3.560 2.183 5 100% purified water 3.870 1.180

It was found that the content of chlorogenic acid increased when extracted using 100% purified water and 100% ethanol with a single solvent rather than with a single solvent. Since the content of chlorogenic acid in the ethanol aqueous solution does not have a big difference, it is preferable to extract with 70 to 85% aqueous ethanol solution in consideration of the time of concentration.

In addition, as the purified water content increased during reflux extraction, high heat was applied, and the color of the extract with high heat became dark. Therefore, it is preferable to extract at room temperature or cold at the time of preparation of the extract.

<Extraction Examples 6 to 8>

400 kg of crushed organza stems were put into 400 L of 80% ethanol aqueous solution, and extracted by stirring at room temperature for 3 days, filtered through 300 mesh filter cloth, and the filtrate was concentrated under reduced pressure at 40 ° C. using a rotary evaporator. This was again redissolved in an aqueous 80% ethanol solution and allowed to stand at room temperature overnight, and then the supernatant was concentrated under reduced pressure at 40 ° C. using a rotary evaporator to obtain crude extract (5.32 kg). Next, the total amount of the extract was filled with a column of 40 cm in diameter and 2 m in length, filled with 10% ethanol aqueous solution, equilibrated with 10% ethanol aqueous solution, and then 10% ethanol aqueous solution, 20% ethanol aqueous solution, 40% ethanol aqueous solution and 60 Elution of 350 L each of the aqueous solution of ethanol in sequence and fractionated 20 L. At this time, 10% (Extraction Example 6), 20% (Extraction Example 7) and 40% (Extraction Example 8) Aqueous ethanol aqueous solution was concentrated under reduced pressure at 40 ℃ in a rotary evaporator to obtain the Ogapi powder, high-performance liquid chromatography The contents of chlorogenic acid, caffeic acid and acantoside D, which are the main constituents of organopi, were measured and shown in Table 2.

Extraction Example Fraction extraction solvent content % Chlorogenic acid Cafe Iksan Acantoside D 6 10% ethanol aqueous solution   3.020 ± 0.080 - - 7 20% ethanol aqueous solution   7.811 ± 0.059 0.394 ± 0.008 - 8 40% ethanol aqueous solution   - 12.270 ± 0.360 1.670 ± 0.140

Chloroic acid was eluted from the latter part of 10% ethanol, and chlorogenic acid was no longer eluted by the latter part of 20%.

<Indicator component content control examples 1 to 4>

When the solvent fractionation to the diion HP20 in the process of the extraction examples 6 to 8 sequentially eluted 350 L with 10% ethanol aqueous solution, 20% ethanol aqueous solution, 40% ethanol aqueous solution and 60% ethanol aqueous solution sequentially 20 L each Small fractions were made. That is, 10 small fractions of 10% ethanol solution, 20%, 40% and 60% ethanol solutions were divided into 20 small fractions, and each of the small fractions (total 70) was measured for drying. The content of chlorogenic acid, which is a major component, was measured. The content of chlorogenic acid in the middle portion of the 20% ethanol aqueous solution was eluted as high as 30 to 40%. The content of chlorogenic acid was determined by combining a small fraction of 20% ethanol with a high content of chlorogenic acid with a small fraction of 10% and 40% ethanol, respectively. The mixture was adjusted as described above and concentrated under reduced pressure at 40 ° C. with a rotary evaporator to obtain an organic powder.

Indicator component content control example Chlorogenic Acid Content (%) One 5.208 ± 0.016 2 9.971 ± 0.061 3 15.194 ± 0.622 4 20.500 ± 1.150

Experimental Example 1 Antioxidant Effect Test

In order to examine the antioxidant effects of the extracts of the extracts prepared in Examples 1 to 4 of the indicator component content control of the present invention, a free radical elimination test and an active oxygen elimination test were performed.

The free radical scavenging test is a modification of the method of Kim et al. (Kor. J. Pharmacogn., 24 (4), 299 ~ 303, 1993), and the stable free radical DPPH (1,1-Diphenyl-2- picrylhydrazyl radical) Was used. Each extract sample obtained according to the indicator component control examples 1 to 4 in 0.2 mM DPPH solution was diluted to an appropriate concentration in methanol and mixed in 2 mL, and allowed to stand at room temperature for 10 minutes before absorbance at 517 nm. Measured. The control group was measured in the same manner by adding methanol instead of the sample solution, and using the methanol instead of DPPH solution to obtain a color correction value for each of the extracted sample and the control.

By using the following equation 1 to calculate the free radical removal rate as a numerical value is shown in Table 4 below. In Table 4 below, IC ₅₀ is a concentration of a sample required to achieve 50% free radical scavenging ratio, which is a value used when comparing with other component substances.

The active oxygen scavenging experiment was a modification of the method of Noro et al. (Chem. Pharm. Bull., 31, 3984 ~ 3987, 1983), using an active oxygen generating system by the xanthine / xanthine oxidase enzyme reaction. The change of absorbance due to oxidation of nitroblue tetrazolim (NBT) by active oxygen was measured.

2.4 mL of Na ₂ CO ₃ , 0.1 mL of xanthine, 0.1 mL of EDTA (ethylenediaminetetraacetic acid), 0.1 mL of BSA (bovine serum albumin), 0.1 mL of NBT, and 0.1 mL of sample were mixed well and allowed to stand at 25 ° C. for 10 minutes. After adding 0.1 mL of xanthine oxidase and reacting at 25 ° C. for 20 minutes, 6 mM CuCl ₂ was added to stop the reaction. Thereafter, the absorbance was measured at an optical wavelength of 560 nm. For the control group, purified water was added instead of the sample solution, and the same method was used. Purified water was used instead of the xanthine oxidase solution to obtain color correction values for each of the extracted sample and the control.

Using the following equation 2, the oxygen scavenging rate is calculated and shown as a numerical value. In Table 4 below, IC ₅₀ is a concentration of a sample required to achieve 50% of an oxygen scavenging rate, and is a value used in comparison with other component substances. The smaller the value, the higher the scavenging ratio.

In Table 4, the antioxidant effects of ascorbic acid (L-ascorbic acid) and tocopherol (dl-alpha-tocopherol) were compared with the extract samples of the indicator components content control Examples 1 to 4 of the vitamins.

sample Free radical scavenging, IC ₅₀ (ug / mL) Free radical activator, IC ₅₀ (ug / mL) Indicator component content control example 1 48.210 ± 0.558 422.333 ± 7.930 Indicator component content control example 2 30.104 ± 0.380 257.778 ± 7.930 Indicator component content control example 3 25.173 ± 0.375 188.362 ± 3.764 Indicator component content control example 4  1.748 ± 0.098 131.419 ± 2.235 Chlorogenic acid  6.692 ± 0.172  38.919 ± 1.523 Ascorbic acid  3.018 ± 0.167   - Tocopherol 18.316 ± 0.030 225.327 ± 4.278

As shown in Table 4, chlorogenic acid, which is the main component of the extract of Ogapi, is superior to tocopherol, and has an antioxidant effect equivalent to ascorbic acid. In addition, it was found that the antioxidant effect of Ogapi extract increased depending on the content of chlorogenic acid. In particular, the indicator component content control Example 4 was found to be superior in free radical scavenging ability than chlorogenic acid as a main component and ascorbic acid as a comparative antioxidant.

Experimental Example 2 Cell Viability Test

In order to verify the primary safety as a raw material of cosmetic extract (marker content control Example 4) and chlorogenic acid as a cosmetic raw material, cell survival rate evaluation experiment was performed using B16F1 (C57BL / 6-derived mouse melanocytes). B16F1 used in this Experimental Example was used from the American Type Culture Collection (ATCC).

Cells were inoculated at a density of 1 × 10 ⁴ cells in DMEM (Dulbecco's Modified Eagle's Medium) with 10% bovine serum and incubated at 37% for 5% CO ₂ for one day. Samples were diluted and treated in cell culture medium for each concentration, followed by incubation for 2 days, followed by addition of dye for cell staining (MTT, 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide), 4 Incubate further for hours. The chromosome crystals were dissolved in dimethyl sulfoxide and measured for absorbance at 540 nm for relative comparison. As a control, a culture medium without a sample was used. Using the following equation (3) to calculate the viability of the cell numerical value is shown in Table 5 below.

sample Cell survival rate (%) 0 ug / mL 15 ug / mL 50 ug / mL 100 ug / mL 500 ug / mL Indicator component content control example 4 100 80.141 76.500 82.499 75.962 Chlorogenic acid 100 84.940 82.706 84.485 81.175

As shown in the results shown in Table 5, the indicator component content control Example 4 and chlorogenic acid was found to be excellent in safety, showing a cell viability of 75% or more even at a high concentration of 500 ug / mL.

Experimental Example 3 Inhibition of Melanogenesis in Cells Using B16F1 Melanosite

As a method for assaying the whitening effect of the above-described indicator component content control Example 4 and chlorogenic acid, the whitening effect was determined by measuring the melanogenesis inhibition rate for B16F1 melanocytes. B16F1 melanocytes used in the present experimental example is a cell strain derived from a mouse and secretes a black pigment called melanin. Samples were treated during the artificial culture of these cells to evaluate the degree of melanin black pigment reduction. The B16F1 melanocytes used in this Experimental Example were used after being sold from the American Type Culture Collection (ATCC).

Melanogenesis inhibitory effect of B16F1 melanocytes was measured as follows. B16F1 melanocytes were inoculated in DMEM (Dulbecco's Modified Eagle's Medium) with 10% fetal bovine serum and 1% antibiotic at a density of 1 × 10 ⁵ cells and incubated at 5% CO ₂ , 37 ° C. for ₁ day. . Thereafter, the samples were treated with 10 mg / mL in DMEM 10% treated with alpha-MSH (melanocyte stimulating hormone, 0.2 uM) and incubated for 48 hours. The set to measure the melanin content after incubation, the cells were separated by trypsin-EDTA and centrifuged to recover the cells. Intracellular melanin quantification was performed with a slight modification of the method of Lotan (Cancer Res., 40: 3345-3350, 1980). Melanin extracted by adding 1N NaOH (10% DMSO) to the cell pellet was dissolved, and the absorbance of melanin was measured at 490 nm using a microplate reader, and melanin was quantified using a melanin standard curve. Quantified melanin was expressed by correction with total protein. As a control, a culture medium without a sample was used. Using the following equation 4 to calculate the melanin inhibition rate is shown in Table 6 below. In Table 6 below, arbutin known as a whitening agent was used as a control to evaluate the whitening effect of the indicator component content control Example 4 and chlorogenic acid.

sample  Melanin content (%) Control 100 Indicator component content control example 4 80.719 ± 10.648 Chlorogenic acid 72.700 ± 16.884 Arbutin 77.846 ± 19.554

As shown in Table 6, the indicator component content control Example 4 and chlorogenic acid showed a similar whitening effect as Arbutin known as a whitening agent even at a concentration of 10 mg / mL.

<Formulation Example 1 and Comparative Example 1>

The ingredient composition of the nutrient skin including the extract of Ogapi according to the indicator component content control Example 4 was prepared as shown in Table 7. At this time, the unit of component content is weight%.

number ingredient Formulation Example 1 Formulation Comparative Example 1 One Purified water Balance Balance 2 Carbomer 0.05 0.05 3 Hydroxyethyl cellulose 0.05 0.05 4 Butylene Glycol 3.0 3.0 5 glycerin 2.0 2.0 6 Fiji-1500 0.5 0.5 7 ethanol 4.0 4.0 8 Polysorbate 80 0.2 0.2 9 Triethanolamine 0.05 0.05 10 antiseptic a very small amount a very small amount 11 Ogapi extract according to the indicator component content control Example 4 0.2 －

Among the components identified by component number in Table 7, components 2 and 3 were first dispersed and dispersed in component 1 (purified water), and then components 4 to 6 were added, and components 8 to 10 dissolved in component 7 and fractionated. Component 11 dissolved in component 4 was added sequentially to prepare a mixture.

Comparative Example 1 for Formulation Example 1, except for the extract of Ogapi according to the indicator component content control Example 4 which is component 11, the same as the ingredient composition or the manufacturing method was set to proceed in the same manner.

<Formulation Example 2 and Comparative Example 2>

The ingredient composition of the nutrient lotion containing the extract of Ogapi according to the indicator component content control Example 4 was prepared as shown in Table 8. At this time, the unit of component content is weight%.

number ingredient Formulation Example 2 Formulation Comparative Example 2 One Cetearyl Alcohol 1.0 1.0 2 Glyceryl Stearate 0.7 0.7 3 Polysorbate 60 1.2 1.2 4 Sorbitan sesquioleate 0.3 0.3 5 Cetyloctanoate 6.0 6.0 6 Squalane 4.0 4.0 7 Macadamia Nut Oil 3.0 3.0 8 Butylene Glycol 4.0 4.0 9 glycerin 4.0 4.0 10 Carbomer 0.15 0.15 11 Xanthan Gum 0.03 0.03 12 antiseptic a very small amount a very small amount 13 Purified water Balance Balance 14 Arginine 0.15 0.15 15 Ogapi extract according to the indicator component content control Example 4 0.5 －

Among the components identified by each component number in Table 8 above, components 1 to 7 were first dissolved by heating at a temperature of 70 ° C., and then components 8 to 12 were dissolved and dispersed in component 13, and emulsified by heating to 70 ° C. Thereafter, the emulsified product was neutralized with component 14, cooled to a temperature of 56 ° C, and then, component 15 dissolved in the fractionated component 8 was added, stirred, and cooled at room temperature to prepare.

Comparative Example 2 with respect to Formulation Example 2, except for the extract of Ogapi according to the indicator component content control Example 4 which is component 15, the same as the ingredient composition or manufacturing method was set to proceed in the same manner.

<Formulation Example 3 and Comparative Example 3>

Component composition of the nutrient essence containing the extract Ogapi according to the indicator component content control Example 4 was prepared as shown in Table 9. At this time, the unit of component content is weight%.

number ingredient Formulation Example 3 Formulation Comparative Example 3 One Purified water Balance Balance 2 Carbomer 0.2 0.2 3 Hydroxyethyl cellulose 0.1 0.1 4 Butylene Glycol 8.0 8.0 5 glycerin 6.0 6.0 6 Sodium hyaluronate 150 15.0 7 ethanol 4.0 4.0 8 Polyoxyethylene Cured Castor Oil 0.1 0.1 9 Triethanolamine 0.2 0.2 10 antiseptic a very small amount a very small amount 11 Ogapi extract according to the indicator component content control Example 4 5.0 -

Of the components distinguished by each component number in Table 9, components 2 and 3 were first dispersed and dispersed in component 1 (purified water), and then components 4 to 5 and 6 were added, and components 8 to 10 were dissolved in component 7. And component 11 dissolved in aliquot component 4 were added sequentially to prepare a mixture.

Comparative Example 3 for Formulation Example 3, except for the extract of Ogapi according to the indicator component content control Example 4, which is component 11, the same as the ingredient composition or manufacturing method was set to proceed in the same manner.

<Formulation Example 4 and Comparative Example 4>

Component composition of the nutrient cream containing the extract Ogapi according to the indicator component content control Example 4 was prepared as shown in Table 10. At this time, the unit of component content is weight%.

number ingredient Formulation Example 4 Formulation Comparative Example 4 One Cetearyl Alcohol 1.5 1.5 2 Glyceryl Stearate 1.0 1.0 3 Polysorbate 60 1.2 1.2 4 Sorbitan sesquioleate 0.3 0.3 5 Cetyloctanoate 6.0 6.0 6 Squalane 8.0 8.0 7 Apricot Kernel Oil 4.0 4.0 8 Dimethicone 1.0 1.0 9 Butylene Glycol 5.0 5.0 10 Grill serine 4.0 4.0 11 Magnesium Aluminum Silicate 0.2 0.2 12 Xanthan Gum 0.05 0.05 13 antiseptic a very small amount a very small amount 14 Purified water Balance Balance 15 Ogapi extract according to the indicator component content control Example 4 1.0 －

On the other hand, among the components identified by each component number in Table 10 above, components 1 to 8 were first dissolved by heating at a temperature of 70 ° C, and then components 9 to 13 were dissolved and dispersed in component 14 and emulsified in heating to 70 ° C. . Thereafter, the emulsified product was cooled to a temperature of 56 ° C., and then component 15 dissolved in the fractionated component 9 was added thereto, stirred, and cooled to room temperature to prepare.

Comparative Example 4 with respect to Formulation Example 4, except for the extract of Ogapi according to the indicator component content control Example 4 which is component 15, the same as the ingredient composition or the manufacturing method was set to proceed in the same manner.

<Formulation Example 5 and Comparative Example 5>

The ingredient composition of the nutritional pack containing the extract Ogapi according to the indicator component content control Example 4 was prepared by configuring as shown in Table 11. At this time, the unit of component content is weight%.

number ingredient Formulation Example 5 Formulation Comparative Example 5 One Purified water Balance Balance 2 Butylene Glycol 3.0 3.0 3 glycerin 2.0 2.0 4 Polyvinyl alcohol 2.0 2.0 5 Polyvinylpyrrolidone 10.0 10.0 6 Cellulose gum 0.3 0.3 7 Mica 2.0 2.0 8 kaoline 2.0 2.0 9 ethanol 5.0 5.0 10 Polyoxyethylene Cured Castor Oil 0.3 0.3 11 antiseptic a very small amount a very small amount 12 Ogapi extract according to the indicator component content control Example 4 2.0 -

Of the components identified by each component number in Table 11 above, components 2 and 3 were first added to component 1 (purified water), and then components 4 to 6 were added and heated to 70 ° C. while stirring and dispersing. The following components 7-8 were added, fully dispersed and cooled to a temperature of 56 ° C. A substance prepared by dissolving components 10 to 11 in component 9 and component 12 dissolved in aliquoted component 2 in turn was stirred and cooled to room temperature.

Comparative Example 5 with respect to Formulation Example 5, except for the extract of Ogapi according to the indicator component content control Example 4 which is component 12, the same as the ingredient composition or the manufacturing method was set to proceed in the same manner.

<Experiment 5> Skin whitening effect and skin irritation sensory test

In order to evaluate the skin whitening effect and skin irritation of the cosmetic composition for skin whitening according to the present invention, a sensory test was carried out using the nutrition cream prepared in Formulation Example 4 and Comparative Example 4.

Specifically, in order to measure the whitening effect of the skin when the nourishing creams of Formulation Example 4 and Formulation Comparative Example 4 were applied to the skin, the nutritional cream of Formulation Example 4 was applied to 20 women over 20 years of age. Group), the right side of the face was allowed to continuously use the nutrition cream (control) of the formulation Comparative Example 4 once a day for 12 weeks.

In the sensory test, the whitening effect of skin was evaluated based on the nutrition cream of Formulation Comparative Example 4, and the whitening effect of the nutrition cream of Formulation Example 4 was relatively evaluated. And phenomena such as erythema. Evaluation was performed based on the five-point standard of very good (5 points), excellent (4 points), moderate (3 points), bad (2 points), very bad (1 point), the results are shown in Table 12 Indicated. In Table 12, the skin irritation indicates the degree of no skin irritation.

number Skin irritation Whitening effect Formulation Example 4 Formulation Comparative Example 4 One 4 5 5 2 5 5 5 3 5 4 5 4 3 4 4 5 4 4 5 6 5 5 5 7 4 4 4 8 4 4 4 9 5 5 5 10 4 4 4 11 5 5 5 12 5 5 5 13 3 4 4 14 4 4 4 15 5 4 4 16 4 4 4 17 5 5 5 18 5 5 5 19 5 5 5 20 4 4 4 Average 4.40 4.45 4.55

As shown in Table 12, the skin irritation evaluation score for the cosmetic composition of the formulation of Example 4 according to the present invention was evaluated very good as 4.40, the skin irritation degree is low as in Comparative Formulation Example 4 is excellent skin safety Could confirm. In addition, the relative skin whitening effect of the cosmetic composition of Formulation Example 4 compared to Formulation Comparative Example 4 was found to be very excellent in the degree of improvement to an evaluation score of 4.55.

Experimental Example 6 Skin Safety Experiment

In order to determine the occurrence of irritation or allergic reactions by observing the skin safety, that is, the skin reaction for the whitening extract and the formulation example 4 according to the indicator component content control Example 4, the skin safety test method of the Korea Food and Drug Administration According to the human patch test.

Thirty healthy men and women aged twenty to fifty were selected as subjects. The patch test was carried out on the inner side of the subject's upper arm and the patch was removed after 48 hours. The first reading was performed after about 60 minutes of stability, and the second reading was taken after 96 hours of patch application. Criteria are as shown in Table 13 below.

reaction weight Criteria of Judgment - 0 No response ± 0.5 Faint erythema + One Boundary but weak erythema, edema and papules ++ 2 Pronounced erythema, papules and blisters

The results read at 48 hours and 96 hours after patch application were calculated using the following equation (5).

The skin irritation degree of the sample showing the positive response calculated based on the test result of the patch test is shown in Table 14 below.

sample 48 hours 96 hours Responsiveness (%) + ++ + ++ 48 hours 96 hours Average Indicator component content control Example 4 (0.2% in distilled water) - - - - 0.00 0.00 0.00 Indicator component content control Example 4 (1.0% in distilled water) - - - - 0.00 0.00 0.00 Formulation Example 4 - - - - 0.00 0.00 0.00

As shown in Table 14, each of the indicator component content control Example 4 and Formulation Example 4 according to the skin reactivity test criteria according to Table 15 in the human patch test was found to be good results for use on sensitive skin without irritation . Therefore, the extract of Ogapi according to the extraction examples appeared as a safe ingredient with no skin side effects as a natural product.

Average responsiveness of the skin Criteria 0.0 to 0.9 Non-irritating 1.0 to 2.9 Light stimulation 3.0 to 4.9 Heavy stimulation 5.0 or more Strong stimulation

Claims (3)

Milling the Acanthopanacis Cortex; Extracting room temperature or cold extraction with 70-85% ethanol aqueous solution; Concentrating the extraction filtrate at 20 to 50 ℃ to obtain a crude extract; Filling the crude extract with ion resin column chromatography and sequentially increasing the content of ethanol to 10 to 60% and eluting with an aqueous ethanol solution to obtain each fraction; And a cosmetic composition for skin whitening containing the extract of Acanthopanacis Cortex prepared through the step of combining a 10 to 40% ethanol aqueous solution fraction of the obtained fraction as an active ingredient. According to claim 1, wherein the composition is a cosmetic composition for skin whitening, characterized in that any one selected from the group consisting of lotion, essence, skin, lotion, cream, pack, foundation and makeup base. (S1) crushing the Acanthhopanacis Cortex; (S2) a step of extracting room temperature or cold extraction with 70-85% ethanol aqueous solution; (S3) extracting the filtrate under reduced pressure at 20 to 50 ° C. to obtain a crude extract; (S4) filling the crude extract with ion resin column chromatography and sequentially increasing the content of ethanol to 10 to 60% and eluting with an aqueous ethanol solution to obtain each fraction; And (S5) combining the 10 to 40% ethanol aqueous solution fraction of the obtained fractions Method for enhancing the whitening effect of the extract (Acanthopanacis Cortex), characterized in that it comprises.