KR100965085B1 - Composition for cosmetic application having skin whitening function - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin whitening containing baekhwasasulcho extract and rhubarb extract as an active ingredient. Using the composition can provide a cosmetic composition that is safe for the skin and excellent in whitening effect.

White flower, rhubarb, whitening, extract, melanin

Description

Composition for cosmetic application having skin whitening function}

The present invention relates to a cosmetic composition for skin whitening. More specifically, the present invention relates to a cosmetic composition for skin whitening containing a natural extract.

The skin has physical and chemical ultraviolet protection factors and has a function to minimize skin disorders caused by various photochemical reactions. The stratum corneum attenuates its energy by reflecting and diffusing ultraviolet light. In addition, melanin, superoxide dismutase (SOD), and other antioxidants absorb ultraviolet light that penetrates the skin and protect the skin from obstructions by reducing its energy or by eliminating free radicals secondary to ultraviolet light. do.

However, various skin disorders occur when the living body receives a large amount of ultraviolet rays exceeding the above-described defensive factors or decreases as the body ages.

There are many cells in the skin that are immune to the body, including macrophages and Langerhans cells. Irradiation of ultraviolet light causes these cells not only to decrease numerically but also to functionally impair. The largest part of skin color determinants is the distribution and amount of melanin in the skin. Melanin is produced in melanocytes, which contain enzymes such as tyrosinase, which work together to polymerize and oxidize tyrosine, an amino acid that is always present in vivo. As a result, melanin, a dark brown pigment, is formed. The melanin thus formed migrates to epidermal cells called keratinocytes through dendritic processes of melanocytes. Here, melanin plays an important role such as forming a hat-like structure around the nucleus to protect genes from ultraviolet rays and to remove free radicals to protect intracellular proteins.

There are no enzymes that break down melanin in vivo, but they are removed by falling off the skin as keratinocytes fall off the epidermis. However, if more melanin is produced than necessary, it causes hyperpigmentation such as blemishes, freckles, and spots, resulting in cosmetically bad results.

Several factors affect melanin production, and the increase of melanin production caused by ultraviolet light and the subsequent pigmentation are very important in the cosmetic field. The basic mechanisms of drugs formulated in whitening cosmetics for the purpose of preventing pigmentation include: inhibiting tyrosinase action, inhibiting tyrosinase production, inhibiting melanin production mediators, reducing melanin reduction and photooxidation, promoting melanin excretion, And ultraviolet cut.

In response to the desire of women to have white skin even under UV exposure, there is an increasing need for the prevention and improvement of skin pigment abnormalities and hyperpigmentation. Many efforts have been made. Specific examples thereof include inhibitors that inhibit tyrosinase activity such as kojic acid, arbutin, hydroquinone, vitamin A, vitamin C, and derivatives thereof. However, they are limited in their use due to safety problems on the skin, stability problems in the formulation and insufficient whitening effects. On the contrary, researches on raw materials derived from natural products that are high in skin whitening and safe for skin are being actively conducted.

An object of one embodiment of the present invention, to provide a cosmetic composition for skin whitening.

It is an object of another embodiment of the present invention to provide a whitening composition that is safe for skin.

Another object of an embodiment of the present invention is to provide a whitening composition having high formulation stability.

Another object of one embodiment of the present invention, to provide a composition for suppressing the skin pigmentation phenomenon such as blemishes, freckles, blemishes.

The cosmetic composition for skin whitening according to one embodiment of the present invention is characterized by containing baekhsasachocho extract and rhubarb extract as an active ingredient.

Using the present invention, it is possible to provide a cosmetic composition that is safe for skin and excellent in whitening effect. In particular, the formation of cutaneous melanocytes can be suppressed and skin pigmentation such as blemishes, freckles, and spots can be prevented or improved. In addition, it is possible to obtain an effect of improving the overall skin tone and improving skin cleanness and dullness.

Oldenlandia diffusa used as an active ingredient in the present invention ( Oneenlandia diffusa) is one of the herbal medicines that have been widely known and used as well as folk medicine. White iris is a plant of Rubiaceae which grows in the lush and shady forest of southern China, Southeast Asia and Jeju Island. Ingredients included in Bacteria (Betulin), Betulinic acid, Ursolic acid, Oleanolic acid, Stigmasterol, Beta-Sitosterol , Beta-glycyrretinic acid, penta-coumaric acid, glucoside, and entriacontane, which have anti-inflammatory, detoxification, antioxidant and antibacterial effects It is known.

Baeksasasulcho extract preparation used in the present invention is not particularly limited and may be prepared according to any one of the methods used in the art. For example, the dried chlorosis powder is added to purified water warmed to 60 to 80 ℃ washed occasionally for 10 to 20 minutes while stirring. The washed baekhwasachocho starch is added to purified water warmed to 80 ~ 85 ℃ extracted occasionally while stirring for 2 to 4 hours. The extracted liquor can be cooled to 20 to 30 ° C., filtered, and solids are removed to obtain white chlorosis extract.

Baeksasasulchocho extract prepared as described above is 0.001 to 10% by weight, preferably 0.005 to 5.0% by weight based on the total weight of the composition. When the concentration was less than 0.001% by weight, no clear effect could be expected. When the concentration was higher than 10.0% by weight, no apparent effect was increased with the increase of the content.

Rhubarb is the root stem of Rheum palmatum Linne, Rheum tanguticum Maximowicz, Rheum officinale Baillon, Rheum coreanum Nakai, or their species hybrid (China). It is a perennial herb distributed on the Korean peninsula. It is also the root of perennial Rheum officiale BAILLON, Rheum palmarum LINNE var. Palmatum or Rheum undulatum LINNE. The main ingredients include Emodin, Chrysophamol, Rhein, Aloe-emodin, Glucogallin and Raponticin. It is known to have pharmacological effects such as antibacterial action, antiviral action, immune complex purification promotion action, hepatocellular interference inhibition action and bacterial collagen degrading enzyme inhibition action.

Rhubarb extract used as an active ingredient in the present invention is not particularly limited and may be prepared by any one of the methods used in the art. For example, dried rhubarb is added to ethanol and extracted by immersion for 5 days while stirring occasionally. The solution is filtered to remove solids and distilled under vacuum to obtain an ethanol mixture. Purified water is mixed with this solution to precipitate and filtered. This solution may be vacuum dried to obtain rhubarb extract powder.

Rhubarb extract prepared as described above is characterized in that it contains an amount of 0.001 to 5.0% by weight based on the total weight of the composition. If the concentration is less than 0.001% by weight, the expected effect may not be achieved, and if it is more than 5.0% by weight, it is not suitable for cosmetics due to discoloration of the contents and occurrence of precipitation in the formulation.

The cosmetic composition of the present invention is not particularly limited in the formulation, for example, a cosmetic composition having a formulation of a flexible cosmetic water, nutrient cosmetics, milky lotion, massage cream, nutrition cream, pack, patch, gel, stick, spray cosmetics Can be.

Hereinafter, an Example and a test example are given and this invention is demonstrated more concretely. However, the scope of the present invention is not limited to the following examples, test examples and the like.

Preparation Example 1: Preparation of Pear Blossom

100 g of dried baekhwasachocho starch was added to 0.8 L of purified water warmed to 70 ° C. and washed occasionally with stirring. Washed Baekhwasajeol outpost was added to 1L of purified water warmed to 80 ~ 85 ℃ extracted occasionally while stirring for 3 hours. The extract was cooled to 25 ° C., filtered, and the solids were removed to obtain about 1 L of chlorophyll extract in the liquid phase.

Preparation Example 2: Preparation of Rhubarb Extract

1 kg of dried rhubarb was added to 6 L of ethanol and then extracted by immersion for 5 days while stirring occasionally. The solution was filtered to remove solids and distilled under vacuum to obtain an ethanol mixture of about 1 L. 1L of purified water was mixed with this solution to precipitate and filtered. The solution was vacuum dried to obtain 30 g of rhubarb extract in powder form.

Preparation Example 3: Preparation of Complex Active Ingredients

BD complex I, II and III were prepared according to the composition of Table 1 below. Each complex was mixed with whitening and white rhubarb extract and rhubarb extract in a specific weight ratio.

Test Example  1: melanin production inhibitory effect measurement

Minimum HM3KO cells (Y. Funasaka, Department of dermatology, Kobe university school of medicine, 5-1 Kusunoki-cho 7-chrome, Chuo-ku, Kobe 650, Japan), human melanoma cells Incubated in Essential Medium (MEM) at 37 ℃, 5% CO ₂ conditions. The cultured cells were spread over 75 flasks so that the number of cells was 3 × 10 ⁵ per flask, waited for the cells to adhere to the wall for one night, and then confirmed that the cells adhered well. Changed to a new badge that contains. As a control, a medium containing DMSO was used. In this way, every two to three days, the cells were transferred to a new medium containing samples, and the cells were incubated until the flask was filled. When the cells were grown, the cells were collected and compared with those of the control and the treated cells. In addition, the culture medium was removed, washed with PBS, dissolved with 1N sodium hydroxide, and measured for absorbance at 500 nm, and then the melanin production inhibition rate was calculated according to Equation 1 shown in Table 1 below.

Melanin inhibition rate (%) = 100-(absorbance of each test substance / absorbance of control X 100)

Test substance Melanin production inhibition rate (%) Baekhwasasulcho extract (Preparation Example 1) 21.7 Rhubarb Extract (Manufacturing Example 2) 29.1 Kojisan 28.5 BD complex Ⅰ
[Waxia vulgaris extract (Production Example 1): Rhubarb extract (Production Example 2) weight ratio = 7: 3] 29.8 BD complex Ⅱ
[Waxia vulgaris extract (Production Example 1): Rhubarb extract (Production Example 2) weight ratio = 5: 5] 34.5 BD complex Ⅲ
[Waxia vulgaris extract (Production Example 1): Rhubarb extract (Production Example 2) weight ratio = 3: 7] 35.3

From the results of Table 1, the whitening effect was obtained when the BD complex was used as compared to the case of using only the whitening or the rhubarb extract alone. In addition, BD complexes I, II, and III all had better whitening effects than kojic acid, which is known to have excellent whitening effect. In other words, the use of whitening and white rhubarb extract alone or rhubarb extract alone when combined with the use of more synergistic effect of the whitening effect was obtained.

Test Example  2: Evaluation of the whitening effect at the animal level

Brown motnea pigs (Tortoiseshell guinea pigs) are animals that cause pigmentation due to ultraviolet rays, PUVA, allergic reactions, etc., like humans, and when hair and skin color are the same, After removing the hair, a wide range of areas can be used. Pigmentation is produced by the method enumerated below, and the improvement effect of pigmentation can be verified by the method of apply | coating the whitening composition to the pigmentation site | part. In addition, substances whose effects have been recognized by this method are useful in primary in vivo screening methods because they have a high probability of having an effect on evaluation in human ultraviolet pigmented plaques.

1. Preparation of UV Pigment

Preparation of pigmentation by ultraviolet rays (UVB) is made by applying SE lamp (wavelength 290-320nm, Toshiba) after attaching the shading aluminum foil having six square windows of 3cm × 3cm to the skin which removed the hair behind the brown morphet. ) Was irradiated with ultraviolet (UVB). The total irradiation energy amount was 1350 mJ / cm ² . After irradiation, the aluminum foil was peeled off and the whitening effect of the test substance was evaluated by the application method as follows. Pigmentation after UV irradiation appears 2-3 days later, reaching peak after about 2 weeks. It is important that the resulting UV pigments have the same color tone and that no stains occur. Individuals with poor UV pigment formations should not be used for good results.

2. Application of Composition

After ultraviolet irradiation, the composition of Table 2 was applied about 14 days after the pigmentation was the highest, that is, the pigmentation was formed. The application number of times was continued for 50 days twice a day. The composition was dissolved and diluted in a mixed solvent of propylene glycol: ethanol: water = 5: 3: 2, coated with a cotton swab, and prepared a control site for applying only a solvent to other sites. Along with the determination of effects, the cumulative stimulus was also observed.

3. Judgment of the effect

The effect was determined by measuring the degree of black and white of the skin using a color difference meter (Minolta CR2002). L * a * b * colorimeter is used for displaying a color, and in this invention, L * value (brightness) was mainly used as an index. The L * value was calibrated with a standard whiteboard, and the pigmentation part was measured evenly by repeating the measurement five times or more at one site. The difference in skin color (ΔL *) at the start and completion of the application was calculated according to Equation 2 below, which is shown in Table 3. The whitening effect is determined by comparing ΔL * between the sample application site and the control site. When ΔL * value is about 2, the whitening effect of the deposited pigment is clear, and when about 1.5 or more, the whitening effect may be determined. .

L * = L * value 50 days after application-L * value at the start of application

ingredient Content weight% Rhubarb extract 50 White flower iris extract 50

Material Concentration (%) Whitening Effect (ㅿ L) Whitening Composition 1.0% 1.78 Whitening composition 0.5% 1.53 Whitening composition 0.1% 1.17 Kojisan 1.0% 0.95 Control 0.78

From the results of Table 3, it was found that the composition of the present invention had a pronounced whitening of the pigment deposited at 1.0% concentration and a whitening effect even at a concentration of 0.1%.

Production Example 4: Example 1-3 and Comparative Example 1-3

In order to test the whitening effect on human skin, the raw materials 1-9 of Table 4 were mixed and dissolved at 70 ° C. to prepare water parts. In addition, raw material 10-16 was melt | dissolved at 70 degreeC, and it was set as the oil part. The oil part was added to the water part, stirred with a homomixer (Tokushu Kika, Japan) and emulsified first, and the raw material 17 was added to increase. After removing the bubbles, the mixture was cooled to room temperature and used in Examples 1-3 and Comparative Examples 1-3. All values in Table 4 represent weight percent.

ingredient Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Comparative Example 3 1. Purified water To100 To100 To100 To100 To100 To 100 2. Glycerin 5.0 5.0 5.0 5.0 5.0 5.0 3. Butylene Glycol 5.0 5.0 5.0 5.0 5.0 5.0 4. Beta Glucan 4.0 4.0 4.0 4.0 4.0 4.0 5. Hyaluronic Acid Extract 3.0 3.0 3.0 3.0 3.0 3.0 6. Carbomer 0.1 0.1 0.1 0.1 0.1 0.1 7. Rhubarb Extract 0.3 0.5 0.7 1.0 - - 8. White jasminoides extract 0.7 0.5 0.3 - 1.0 - 9. Kojisan - - - - - 1.0 10. Caprylic Capric
Triglycerides 8.0 8.0 8.0 8.0 8.0 8.0 11. Hydrogenated Polydecene 5.0 5.0 5.0 5.0 5.0 5.0 12. Cetearyl Glucoside 1.5 1.5 1.5 1.5 1.5 1.5 13. Sorbitan Stearate 0.4 0.4 0.4 0.4 0.4 0.4 14. Cetearyl Alcohol 1.0 1.0 1.0 1.0 1.0 1.0 15. Preservative Quantity Quantity Quantity Quantity Quantity Quantity 16. Incense Quantity Quantity Quantity Quantity Quantity Quantity 17. Triethanol Amine 0.1 0.1 0.1 0.1 0.1 0.1

Test Example  3: whitening effect test on human skin

Twelve healthy men were placed with opaque tape with six 1.5cm diameter holes in the upper forearm area of the subject, followed by 1.5-2 times the UVB of the Minimal Erythema Dose. The blackening of the skin was induced by irradiating and the test materials were applied and two months later, the contrast of the skin was measured using a color difference meter. The 12 men's skin external preparations of Examples 1-3 and Comparative Examples 1-3 were applied twice daily in the morning and evening.

The determination of the effect was determined by obtaining the "L" value representing the contrast of the skin as in the animal test. For reference, Korean skin color that is not artificially burned generally shows a value of 50-70.

If the test material is effective, the L value is gradually increased, and the comparison between the test materials calculates the difference (ΔL) of the skin color at the start and completion of the coating according to Equation 2, Table 5 shows.

Test substance Whitening Effect (△ L) Example 1 3.19 Example 2 3.52 Example 3 3.75 Comparative Example 1 2.62 Comparative Example 2 2.14 Comparative Example 3 2.26 Control 1.27

As can be seen in Table 5, the whitening effect was higher in the mixture composition of the whitening and red rhubarb extract and rhubarb extract than that of the white and yellow rhubarb extract and rhubarb extract alone, and the whitening effect is better than Koji acid High effect. Based on the results, it can be seen that there is a synergistic effect of the combination of baekhwangsachocho and rhubarb extract in the whitening effect of the composition.

Claims (2)

It contains the extract of Oldenlandia diffusa and Rheum genus as active ingredients, The weight ratio of the baekrye ssaengcho extract and rhubarb extract is 3: 7 to 5: 5 the cosmetic composition for skin whitening, characterized in that. The cosmetic composition for skin whitening according to claim 1, wherein the cosmetic composition for skin whitening comprises 0.001 to 10% by weight of baekrye-sachocho extract and 0.001 to 5.0% by weight of rhubarb extract.