KR20000044979A - Composition for skin whitening comprising portulaca oleracea l. extract - Google Patents

Abstract

PURPOSE: A composition for skin whitening is provided which is excellent in inhibiting effect of melanin production and skin whitening effect by Portulaca oleracea L. extract as the inhibitor of melanin production. CONSTITUTION: A composition comprises Portulaca oleracea L. extract to inhibit the production of melanin. Portulaca oleracea L. extract is manufactured by the following steps: 1) 2 liter of water and 1 liter of 1,3-butylene are added to 1kg of pulverized Portulaca oleracea L. after being washed, and the mixture is shaking for 1-2 weeks, 2) the extract is freeze-dried or spray-dried after the filtration and the concentration.

Description

Skin whitening composition containing pentachoea extract

The present invention relates to a composition for skin whitening containing quincean extract, and more particularly, to a composition for skin whitening having an excellent whitening effect as a skin whitening agent by improving melanin and freckles by inhibiting melanin production.

It is everyone's constant desire to have white, fair skin. Human skin color is genetically determined by the concentration distribution of melanin in the skin, but is also affected by environmental or physiological conditions such as sun, ultraviolet light, fatigue, stress, and the like. Melanin is produced through a non-enzymatic oxidation reaction after changing to dopa (Dihydroxy Phenyl Alanine: DOPA) or dopaquinone by the action of tyrosinase (Tyrosinase) on the amino acid Tyrosine . However, although the pathway through which melanin is made is known, it is still unknown in detail what mechanisms induce melanin synthesis, the previous step in which tyrosinase works. On the other hand, conventionally, by combining a substance having an inhibitory activity against tyrosinase such as hydroquinone, ascorbic acid, kojic acid, glutathione, etc. in ointments or cosmetics, skin whitening It has been used for the purpose of improving blemishes or freckles. However, in the case of hydroquinone, the skin whitening effect is recognized, but the skin irritation is severe, there is a problem that the amount of use is extremely limited, ascorbic acid is easy to be oxidized, causing cosmetics such as discoloration or deterioration. Thiol compounds such as glutathione and cysteine not only have a characteristic unpleasant odor but also have a problem of poor transdermal absorption. In addition, these glycosides and derivatives have a high polarity, there is a problem to use these components as a blending component of the cosmetic. Placenta extract may also be used for this purpose, but this material has a problem that the whitening effect is not excellent.

The present invention is to solve the above problems, to provide a composition for skin whitening safe to the living body and high melanin production inhibitory effect and whitening effect.

In order to achieve the above object, the present invention provides a composition for skin whitening comprising a pentacocci extract having a high melanin production inhibitory effect and a whitening effect.

Hereinafter, the present invention will be described in detail.

The present inventors have studied to develop a new whitening agent for native plants and animals using B-16 mouse melanoma cells that can screen for substances that inhibit melanin synthesis induction itself. As a result, it was found that the peninsula extract has a melanin production inhibitory effect and a whitening effect, thereby completing the present invention.

The skin composition of the present invention comprises a pentacle quince extract, which extract is stirred after adding a conventional organic solvent such as 1,3-butylene glycol or ethanol to the outpost of Portulaca Oleracea L. After extraction for 2 weeks, the primary extraction, concentrated under reduced pressure and then prepared by freeze drying or spray drying.

The five pentagram used in the present invention has the following characteristics.

Pentagram is a fleshy annual herb that grows on the pavement, hairless, 30 cm high, reddish brown, with many branches that spread out at an angle. Roots are white, but when peeled by hand, they become red like a main stem, so children play with them. Leaves are apical or regenerated, but the end is aquatic, obovate, narrow in base, short lobe, 15-25 mm long, 5-15 mm butterfly with flat edges. Flowers are bisexual and continue to bloom from June to autumn, and are yellow and hang on the ends of branches. Sepals are 2, oval, 5 petals, and shriveled. Surgery 7-12, pistil 1, perilla, neutral, 5 styles. Fruits are oval, with a central part split sideways, resulting in many seeds with long stems. Seeds are crushed, round, black, slightly rounded at the edges. In the West, they make salads with lettuce, and in Korea, they eat the soft parts as herbs (Korean Plant Book, Lee Chang-bok, P324, 1993). The Latin name for Pentacho is Portulaca Oleracea L., which is collected between summer and autumn, washed, and then slightly boiled in the sun or used as raw. Peninsula is belonging to Purslane and has been used as a herbal medicine (consensus treatment) for dysentery, enteritis, bacterial isolation, or detoxification of insect poisoning and snake venom due to the action of detoxification, pear abscess, etc., which is the skin of the present invention. It is clearly distinguished from the whitening effect. According to the conventional pentacholine component identification studies, 1-noradrenaline, dopamine, tocopherol, saponin, tannin, and the like have been reported.

The manufacturing method of the five pentachoea extract is as follows.

After purchasing the pentacle vinegar, clean it and grind it finely, add 2 volumes of water and 1 volume of 1,3-butylene glycol (or ethanol) to the pentagram dry weight, and then stir for 5 weeks. Extract the ingredients. The residue in the extract was filtered with a filter cloth, and then concentrated under reduced pressure, followed by freeze drying or spray drying to obtain a dry extract. The extraction temperature is carried out at 10 to ℃, preferably at room temperature. The extract's appearance is a light brown transparent liquid with a characteristic odor. The pentagram extract prepared in this manner showed potent melanin synthesis inhibitory effects in rat melanoma cells (B-16 melanoma cells). In addition, it is possible to apply cosmetics such as supple cosmetics, nourishing cosmetics, nourishing creams, massage creams, essences, and packs with extracts of Machi Prefecture. When the skin whitening effect is not only very good, and also does not cause any side effects, it can be seen that the pentacle quince extract is a safe and effective improvement of blemish or freckles and skin whitening agent.

The content of the quincean extract contained in the preparation of the skin external ointment or cosmetic including the quincean extract is 0.01 to 20.0% by weight, preferably 0.5 to 10.0% by weight of the extract. If the content of the extract is less than 0.01% by weight, no obvious effect can be expected. If the content of the extract exceeds 10.0% by weight, the increase in content did not appear to increase.

The following presents preferred examples and comparative examples to aid in understanding the invention. However, the following examples are merely provided to more easily understand the present invention, and the present invention is not limited to the following examples.

Example 1

After washing and grinding the pentagram, 1 kg of the pulverized powder was added to a mixed solvent of 2 L of water and 1 L of 1,3-butylene glycol, stirred, and extracted at room temperature for about 10 days in an extractor. Filtration was carried out with a micro filter having an extract. The obtained extract was filtered using Whatman No. 2 filter paper at room temperature to remove insoluble matters, and then concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling condenser and freeze-dried to obtain 12.4 g of dry extract as a dry weight.

Example 2

After washing and grinding the pentacle vinegar, 1 kg of the pulverized product was added to a mixed solvent of 2 L of water and 1 L of ethanol, stirred and extracted at room temperature for about 10 days in an extractor, followed by filtration with a micro filter having a pore size of 0.3 μm. An extract was obtained. The obtained extract was filtered with Whatman No. 2 filter paper at room temperature to remove insoluble matters, then heated at 140 ° C. for about 3 hours, concentrated under reduced pressure to 60 ° C. in a distillation apparatus equipped with a cooling condenser, and lyophilized to dryness. The extract obtained 11.8 g in dry weight.

Experimental Example 1

Investigation of Melanin Production Inhibitory Effect at the Cell Level of Pentacinum Extract

The five-fold extract obtained in Example 1-2 was added to the culture solution of mouse melanoma cells (B-16 mouse melanoma cells) to test the whitening effect at the cellular level as follows. The final concentration of each extract obtained in Example 1-2 was added to the culture medium at the time of culturing B-16 melanoma cells, the cells were treated with trypsin after incubation for 3 days, the culture vessel The melanin was extracted after centrifugation from the melanocytes. 1 ml of sodium hydroxide solution (1N concentration) was added to the extracted melanin, boiled for 10 minutes to dissolve melanin, and the absorbance was measured at 400 nanometer (nm) using a spectrophotometer to determine the amount of melanin produced per unit cell allowance (10 ⁶ cell). The absorbance was carried out by the method indicating the absorbance. This experiment was repeated three times and the average value was used to calculate the relative melanin production relative to the control in terms of inhibition rate (%), and the results are shown in Tables 1 and 2 below.

Experimental Example 2

In the same manner as in Experiment 1, the melanoma cells of the rats cultured in the same manner as the hydroquinone and the quincean extract obtained in Example 1-2 while varying the concentration of melanin production inhibition calculated, etc. Table 1 And 2.

sample Melanin production % Inhibition Control group (no addition) 0.044 ± 0.004 - Example 1 (final concentration 0.5%) 0.030 ± 0.001 32% Example 2 (final concentration 0.5%) 0.029 ± 0.001 34% Hydroquinone (final concentration 0.0001%) 0.025 ± 0.002 43%

Iterations = 3

sample Application concentration (%) Melanin production % Inhibition Control group (no addition) - 0.044 ± 0.004 - Example 1 Final concentration (0.01%) 0.041 ± 0.002 7% Final concentration (0.05%) 0.035 ± 0.001 20% Final concentration (0.5%) 0.030 ± 0.001 32% Final concentration (5%) 0.022 ± 0.002 50% Example 2 Final concentration (0.01%) 0.041 ± 0.002 7% Final concentration (0.05%) 0.033 ± 0.001 25% Final concentration (0.5%) 0.029 ± 0.001 34% Final concentration (5%) 0.020 ± 0.001 55% Hydroquinone (0.0001%) 0.025 ± 0.002 43%

Iterations = 3

As shown in the results of Table 2, it can be seen that the pentacle sinensis extract has a strong melanin production inhibitory ability against the cultured melanoma cells. Hydroquinone has a strong inhibitory ability to inhibit melanin production at low concentrations, but is highly cytotoxic and cannot be tested at concentrations above 0.0001%. However, it can be seen that the extract of the present invention has a high melanin production inhibitory effect without showing cytotoxicity even at a concentration of 0.5% or more. In addition, since the pentacle quince extract has a high melanin production inhibitory ability, considering that the mixture is not a single substance, it indicates that the present invention can be very useful for improving blemishes and freckles and skin whitening.

Preparation Example 1 and Comparative Example 1

Each component shown in Table 3 below was mixed in the ratio shown in Table 3 to prepare an external skin ointment. Wherein the pentachopa extracts were obtained in Examples 1-2, respectively.

Composition Preparation Example 1 (% by weight) Comparative Example 1 (% by weight) Pentacle vinegar extract 10 - -Diethyl sebacate 8 8 -Payment 5 5 Polyoxyethylene oleyl ether 6 6 Phosphate Sodium benzoate Quantity Quantity -vaseline Remaining amount * Remaining amount

*: Amount to be 100% by weight in total

Preparation Example 2 and Comparative Example 2

Each component shown in Table 4 below was mixed in the ratio shown in Table 4 to prepare a nourishing cream. Wherein the pentachopa extracts were obtained in Examples 1-2, respectively.

Composition Preparation Example 2 (% by weight) Comparative Example 2 (% by weight) -Changdo Extract 5.0 - Stearyl acid 15.0 15.0 Cetanol 1.0 1.0 Potassium hydroxide 0.7 0.7 -glycerin 5.0 5.0 Propylene glycol 3.0 3.0 -antiseptic Quantity Quantity -incense Quantity Quantity -Purified water Remaining amount * Remaining amount *

*: Amount to be 100% by weight in total

Preparation Example 3 and Comparative Example 3

Each component shown in Table 5 was mixed in the same ratio as shown in Table 5 to prepare a flexible lotion. Wherein the pentachopa extracts were obtained in Examples 1-2, respectively.

Composition Preparation Example 3 (wt%) Comparative Example 3 (wt%) -Pentacle Extract 3.0 - - ethanol 10.0 10.0 -Polylaurin polyoxyethylene 1.0 1.0 Sorbitan - - Paraoxybenzoic Acid Methyl 0.2 0.2 Glycerin 5.0 5.0 1,3-butylene glycol 6.0 6.0 -Incense Quantity Quantity -Pigment Quantity Quantity - Purified water Remaining amount * Remaining amount *

*: Amount to be 100% by weight in total

Preparation Example 4 and Comparative Example 4

Each ingredient shown in Table 6 was mixed in the same ratio as shown in Table 6 to prepare a nourishing lotion. Wherein the pentachopa extracts were obtained in Examples 1-2, respectively.

Composition Preparation Example 4 (wt%) Comparative Example 4 (wt%) -Pentacle Extract 3.0 - Waselin 2.0 2.0 -Sesquioleate sorbitan 0.8 0.8 Polyoxyethylene oleylethyl 1.2 1.2 Paraoxybenzoic Acid Methyl Quantity Quantity Propylene Glycol 5.0 5.0 - ethanol 3.2 3.2 Carboxy vinyl polymer 18.8 18.0 Potassium hydroxide 0.1 0.1 -Incense Quantity Quantity -Pigment Quantity Quantity - Purified water Remaining amount * Remaining amount *

*: Amount to be 100% by weight in total

Preparation Example 5 and Comparative Example 5

Each component shown in Table 7 was mixed in the same ratio as shown in Table 7 to prepare a pack. Wherein the pentachopa extracts were obtained in Examples 1-2, respectively.

Composition Preparation Example 5 (% by weight) Comparative Example 5 (wt%) -Five Elements 2.0 - Glycerin 5.0 5.0 -Propylene Glycol 4.0 4.0 -Polyvinyl alcohol 15.0 15.0 - ethanol 8.0 8.0 Polyoxyethylene oleylethyl 1.0 1.0 Paraoxybenzoic Acid Methyl 0.2 0.2 -Incense Quantity Quantity -Pigment Quantity Quantity - Purified water Remaining amount * Remaining amount *

*: Amount to be 100% by weight in total

Preparation Example 6 and Comparative Example 6

Each component shown in Table 8 below was mixed in the ratio shown in Table 8 to prepare an essence. Wherein the pentachopa extracts were obtained in Examples 1-2, respectively.

Composition Preparation Example 6 (wt%) Comparative Example 6 (wt%) -Pentacle Extract 5.0 - Propylene Glycol 10.0 10.0 Glycerin 10.0 10.0 -Aqueous sodium hyaluronate solution (1%) 5.0 5.0 - ethanol 5.0 5.0 -Polyoxyethylene Cured Castor Oil 1.0 1.0 Paraoxybenzoic Acid Methyl 0.1 0.1 -Incense Quantity Quantity - Purified water Remaining amount * Remaining amount *

*: Amount to be 100% by weight in total

Experimental Example 3

Twenty healthy men and women were selected as subjects, and the lower arm of each arm was covered with two rows of aluminum foil with 6 rows of 7 mm diameter, and 120 mJ with a ORIEL Solar Simulator 1000W at a distance of 50 cm from the arm. The amount of light of / cm 2 was investigated. Twice a day from 3 days before the irradiation to the 4th week after the irradiation, a pair of samples containing the five-fold extract prepared according to Preparation Example 1-6 and Comparative Example 1-6 and the base containing no extract were prepared in pairs. Was applied to. Here, in the case of the pack formulations of Preparation Example 5 and Comparative Example 5 after the application was removed after 15 minutes. For each of the above, the pigmentation degree of the preparation example and the comparative example was visually determined, and the results are shown in Table 9 below.

Pigmentation Inhibitory Effect of Examples According to the Present Invention A distinct effect In effect no difference Preparation Example 1 6 * 8 6 Preparation Example 2 5 9 6 Preparation Example 3 10 6 4 Preparation Example 4 7 7 6 Preparation Example 5 3 8 9 Preparation Example 6 4 8 8

*: Means number of subjects

As shown in Table 9, the whitening ointment and cosmetics containing the five-fold extract of the quincean extract prepared according to 1-6 in the preparation showed a whitening effect on at least 10 of 20 subjects. In addition, it does not show any side effects in the skin, it can be seen that the quincean extract is a skin whitening agent that is safe and has an excellent effect on improving freckle and freckles.

Skin composition containing the pentacle quince extract of the present invention is excellent in solubility and preservation stability and easy to apply, so it can be formulated at high concentrations, and has excellent melanin-producing inhibitory effect, resulting in excellent pigmentation inhibitory effect. It shows the improvement of blemish or freckles and does not cause side effects, so it is excellent in safety.

Claims (3)

A composition for skin whitening containing quincean extract as a melanogenesis inhibitor. The method of claim 1, The five-fold extract is a composition for skin whitening is extracted using at least one mixed solvent selected from the group consisting of water, 1,3-butylene glycol and ethanol. The method of claim 1, The amount of the quincean extract is a skin whitening composition of 0.01 to 20.0% by weight dry weight relative to the total composition.