KR100540463B1 - Composition containing portulaca oleracea L. extract for anti-inflammation and anti-irritation - Google Patents

Abstract

The present invention relates to anti-inflammatory and anti-irritant compositions containing pentachopae extract. The composition of the present invention exhibits excellent effects in cytotoxicity, allergic potential, inflammation inhibition, skin primary stimulation test, etc., and is excellent in solubility and preservation stability when combined with conventional anti-inflammatory agents and is easy to apply.

Description

Composition containing portulaca oleracea L. extract for anti-inflammation and anti-irritation

The present invention relates to anti-inflammatory and anti-irritant compositions. More specifically, the present invention relates to a composition containing portulaca oleracea L. extract, which has excellent anti-inflammatory and anti-irritant effects.

Cosmetics are used to protect the skin and to cleanse beautification, but if you look closely at the cosmetic composition is used because different ingredients are indispensable for product formation. Examples include solubilizers and emulsifiers, preservatives, fragrances, sunscreens and other additives used to impart other beneficial effects. These ingredients are generally known to be the main causes of inflammation, rashes and edema in the skin.

Inflammation is a series of defensive purposes intended to minimize the response of a cell or tissue to a damaged body and to restore the damaged area to its original state.Inflammation causes inflammation, burns, frostbite, and radiation. Chemical factors caused by chemicals such as acids There are immunological factors caused by antibody reactions, as well as vascular or hormonal imbalances. Anti-inflammatory drugs are called anti-inflammatory agents that work to eliminate inflammation, reduce bioreactions and symptoms to eliminate inflammation.

In cosmetics, the cause of skin side effects is always latent and various studies have been conducted to solve this problem. Materials used for anti-inflammatory purposes so far are nonsteroidal flufenamic acid, ibuprofen, benzydamine, indomethacin, and indolecine as steroids. (prednisolone) and dexamethasone (Chang Chang-jong, Pathophysiology, Hallym Co., p61-69, 1988), allantoin, azulene, ε-aminokiproic acid, hydrocortisone (v), licoriceic acid and derivatives thereof β-glycileic acid and glychyl acid derivatives are known to be effective in anti-inflammatory (Ministry of Gwangju, Ministry of Justice, New Cosmetics, Namsan Sugar, p162, 1933), but indometacine cannot be used in cosmetics. Hydrocortisone has a limited amount of usage and licorice acid and its derivatives are difficult to stabilize or have poor solubility. The anti-inflammatory agents known to date have been problematic in terms of skin safety and stability in formulating cosmetics, and their use is limited.

Accordingly, the present inventors have diligently researched to improve the above-mentioned problems of the prior art, and found that pharmaceuticals or cosmetic compositions containing the pentacle vinegar extract have excellent safety on the skin and are easy to stabilize and have a substantial effect upon cosmetic formulation. The present invention has been completed.

The present invention relates to anti-inflammatory and anti-irritant compositions containing the pentacle vinegar extract.

The pentagram is a fleshy annual weed that often grows on the pavement, hairless, 30 centimeters high, reddish red, and many branched branches. Roots are white, but when peeled by hand, they become red like a main stem, so children play with them. Leaves are apical or regenerated, but the apex is aquatic, obovate bean, narrowed at the bottom, short lobe, 15-25mm long, 5-15mm butterfly, with flat edges. Flowers are bisexual and continue to bloom from June to autumn, and are yellow and hang on the ends of branches. Calyxes are 2, oval, 5 petals, and shriveled. Surgery is 7-12, pistil is 1, the room is neutral and 5 styles. Fruits are oval, with a central part split to the side, resulting in many seeds with long stems. Seeds are crushed, round, black, slightly rounded at the edges. In the West, salads are made together with lettuce, and in Korea, soft parts are used as herbs, and outposts are used to relieve the poison of insects and snakes, or as diseases and diuretics (Korean Plant Book, Lee Chang-bok, p324, 1993).

The pentacle vinegar extract is washed with one kilogram of pentagonal vinegar and extracted with a common solvent such as 1,3-butylene glycol, propylene glycol, ethanol, and extracted by adding 2 kg of purified water and 1 kg of solvent.

In the composition of the present invention, if the content of the pentagram vinegar extract is 0.001 parts by weight or less can not be expected a sufficient effect, when more than 30 parts by weight when a problem in the color, smell, usability when formulated into the product. Therefore, in the composition of the present invention, it is preferable that the pentaclecho extract contains 0.001 to 30.0 parts by weight. The composition according to the present invention can be used as a softening lotion, nutrient lotion, essence, nutrition cream or pack.

Experiment 1. Cytotoxicity Test

In order to investigate the cytotoxicity of the pentacle foliar extract used in the present invention, MTT [3- (4,5] -dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide reduction test] was performed and the MTT-formazan product was extracted with isopropyl alcohol to measure absorbance at 570 nm. In order to confirm the IC ₅₀ (inhibition concentration 50) for each substance, the substance was diluted in 10.0%, 1.0% ... in a medium and cultured for 24 hours to obtain an IC ₅₀ .

TABLE 1

Cytotoxicity Test Results of Peninsula Extract

It was confirmed that the five-fold extract was more than 2000-fold lower cytotoxicity compared to sodium lauryl sulfate (SLS).

Experiment 2. Allergy assessment

To confirm the allergy to the pentacle fern extract, apply 25µl sample to the mouse ear once a day for 3 days, remove lymph nodes on day 4, isolate lymphocytes, and incubate for 24-48 hours in 5% carbon dioxide incubator. The extent is measured by the intercalation of the radioisotope [ ³ H] -methyl thymidine. The local lymph node assay (LLNA) results indicate the lymphocyte amplification of the sample group compared to the control group, and allergens when the amplification degree (SI) of the sample is more than three times higher than the control group and increase by concentration Considered as.

TABLE 2

Allergy Evaluation Results of the Peninsula Extract

※ cpm (counter per minute): [ ³ H] -methylthymidine is inserted into the cell

S.I (stimulation index): The average cpm of the sample divided by the average cpm of the control group.

As shown in Table 2, it was shown that the amplification degree (S.I) of the pentacle sinensis extract is less than 3, and there is little possibility of allergy.

Experiment 3. Anti-inflammatory Experiment

In order to examine the anti-inflammatory effect on the pentaflora extract, the mouse's left ear was used as the control site and the right ear as the test site. The ears were washed with ethanol before application of the sample, and 20 μl of the sample was continuously applied once a day for 4 days. One hour after the last application, ethanol was applied to the left ear and arachidonic acid (2 mg / ear) was applied to the right ear. After 1 hour, the ear edema was measured three times by micrometer.

The anti-inflammatory effect was determined as the degree of edema inhibition based on the arachidonic acid treatment group. The results are shown in Table 3.

TABLE 3

Ear thickness and inflammation inhibition rate by topical application

※ Inhibition Rate (%) = (A-B) / A x 100

A: Average thickness of control ears (thickness of arachidonic acid treated ears-thickness of untreated ears)

B: thickness of the sample-coating group ears (thickness of the sample-treated ears-thickness of the untreated ears)

As shown in Table 3, 0.5%, 1.0%, and 3.0% of the pentacobacterium extract showed 15.5%, 20.2%, and 26.6% of inhibition rate, respectively. The significance level of was shown.

Experiment 4. Primary Skin Irritation Test (Closed Wrap Test)

A 50% aqueous solution of quincean extract, 3.0% aqueous solution, and 0.08% sodium lauryl sulfate solution were applied to a healthy adult male and female for 24 hours with a certain amount (0.2 g) of each sample on the back, and then the pin chamber was removed. After a lapse of time, the skin condition was read visually. The results are shown in Table 4.

TABLE 4

Skin primary irritation test result

※ Criteria - ; No erythema or unusual symptoms

+-; Slightly redder than the surroundings

+; Significantly redder than the surroundings

++; Severe redness and swelling than the surroundings

In this way, the anti-inflammatory and anti-irritant effect was excellent, and the following preparation examples were performed using the pentacle quince extract safe for the skin.

Preparation Example 1.

Preparation Example 2.

Preparation Example 3.

Preparation Example 4.

Preparation Example 5.

The pentacle vinegar extract-containing composition according to the present invention exhibits excellent effects in cytotoxicity, allergic potential, inflammation inhibition, skin primary stimulation test, etc., and has superior skin safety and solubility and storage stability when combined with conventional anti-inflammatory agents. Easy to apply Therefore, the composition of the present invention is very useful industrially.

Claims (3)

A composition for external application for skin having an anti-inflammatory or anti-irritant effect, which contains a pentachopa extract. The composition of claim 1, wherein the composition contains 0.001 to 30.0 parts by weight of the pentacle fern extract. The composition according to claim 1, which is softening cream, nutrient cream, essence, nourishing cream or pack.