JPH1045615A - Plant extract-containing fibroblast growth promoter - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain the subject promoter capable of providing a preparation for external use for skin, a bathing agent and food and beverage having promoting action on fibroblast growth and high safety, containing an extract of a plant such as sesame. SOLUTION: This promoter contains an extract of one or more plants selected from seed of Seamum indicum L., rhizome of Dioscorea japonica Thunb., fruit of Capsicum annuum L., rhizome of Angelica acutiloba Kitagawa, whole plant of Houttuynia cordata Thunberg and root of Ophiopogon japonicus Ker-Gawler. The extract is obtained by subjecting the part of the plant to an extraction treatment with a solvent as it is or grinding and subjecting the part to an extraction treatment. To be concrete, for example, seed of Seamum indium L. or rhizome of Dioscorea JaPonica Thunb., is subjected to an extraction treatment with purified water (80 deg.C) for about 5 hours under heating and preferably filtered. The amount of the extract added to the promoter is >=0.01wt.%, especially 0.1-60wt.%.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a novel and safe fibroblast proliferation promoter containing one or more plant extracts selected from sesame, sanyak, capsicum, touki, dokudami and Bakumondou, The present invention also relates to application to various skin external preparations, bath preparations, and foods and beverages in the field of pharmaceuticals, quasi-drugs, or cosmetics.

[0002] The field of application is generally applicable to various internal and external preparations (including preparations used for animals), and specifically, ampules, capsules, pills, tablets, and powders. 1) Pharmaceuticals, 2) Quasi-drugs, 3) Foods, 4) Topical or systemic skin cosmetics, 5) Scalp / hair Pharmaceutical and / or cosmetic preparations (eg, shampoos, rinses, treatments, permanent solutions, hair dyes,
Hairdressing agents, hair tonics, hair growth / hair restoration, etc.), 6) bath agents for use in bath water, 7) other liquid odor / odor control agents, sanitary articles, sanitary cotton, wet tissues, etc. .

In addition, it is used for various injuries such as skin and scalp, oral cavity, eyes, ears, nose, anus, and genital area for the purpose of promoting the growth of skin and dermis, preventing skin aging, roughening the skin and promoting wound healing. be able to.

[0004] Foods and drinks further include oral compositions (gum, candy, etc.), processed seafood products such as kamaboko and chikuwa, livestock products such as sausage and ham, western confectionery, Japanese confectionery, raw noodles, Chinese noodles, and yuden noodles , Buckwheat and other noodles, sauces, soy sauce, sauce, sugar, honey, powdered candy, water syrup, and other spices, curry powder, mustard powder, pepper powder and other spices, jam, marmalade, chocolate spread, pickles, soy sauce, Processed vegetables and fruits such as sprinkles and canned and bottled vegetables and fruits, dairy products such as cheese, butter, yogurt, miso soup, soup, fruit juice, vegetable juice, whey drinks, soft drinks, alcoholic beverages, etc. Use in general foods and drinks such as health foods.

[0005]

2. Description of the Related Art Individual aging or various diseases caused by the aging have a correlation with aging of all cells that can divide (reduction of division rate and cell function). As a result, a large number of cell activators have been searched for. Among them, one of the cell activators is cell growth factor, and various factors have already been confirmed, and the applicant's connective tissue hydrolyzate (Japanese Patent Application Laid-Open No. 62-84024), and water solubility derived from organs such as thoracic line and spleen Protein (Japanese Unexamined Patent Publication No. 63-188697,63
-188698), or bovine placenta extract (JP-A-03-141299) or an extract of bovine brain, bovine pituitary gland, and cockscomb (JP-A-01-175)
998), which are used for quasi-drugs and cosmetics.

[0006]

However, these are all derived from animal systems. As plant-based ones, wolfberry is a cell activator (such as a cell activator, which promotes cell engraftment rate and colony formation). Japanese Patent Application Laid-Open No. 02-117686), but at present, no plant-derived plant having cell growth ability has been obtained.

[0007] In view of these circumstances, the present inventors have developed various plants or components which are useful as fibroblast growth promoters as the theme of development, and as a result, extracted sesame, sanyak, capsicum, touki, dokudami, and bakumondou. The product was confirmed to have a fibroblast growth promoting effect, and
The present invention has been completed by providing a highly safe skin external preparation, bath preparation, food and drink, which is effective for prevention and improvement of various diseases of the scalp (for example, eczema, rough skin, skin swelling, etc.). Was.

[Means for Solving the Problems] BEST MODE FOR CARRYING OUT THE INVENTION

The term "sesame: sesame" used in the present invention is a plant of the sesame family (Pedaliaceae), sesame (Seamum) (Seamum indicum L.) or (Seamum orientale).
L.) Seeds.

"San yak: mountain medicine" is a plant of the family Dioscoreaceae (Dioscoreaceae), a plant of the genus Dioscorea (Dioscorea) (Dioscorea japonica Thunb.) Or a potato "Dioscoreabatatas Decne. (= Dioscorea opposite T).
hunb.) ”.

"Capsicum: chili" means solanaceae (So
lanaceae), a plant pepper of the genus Capsicum (Capsicum) "Capsicum annuum L. = (Capsicum frutescens
L.) ”or a variety or variety thereof. Other congeners (Capsicum annuum L.var.cerasiforme Mill.), (Caps
icum annuum L.var.conoides Irish), (Capsicum ann
uumL.var.fasciculatum Irish), pepper "Capsicum
annuum L.var.angulosum Mill. = (Capsicum annuum Lv
ar.grossum Sendt.) ", (Capsicum annuum L.var.lon
gum Sendt.) can also be used.

"Touki: Toki" means Umbellife (Umbellife)
rae), a plant of the genus Angelica (Angela)
rhizome of ica acutiloba Kitagawa or other closely related plants)
Root bark.

"Dokudami: Jujube": Dokudami (Saurura)
ceae), a plant of the genus Houttuynia (Houtuynia)
ttuynia cordata Thunberg) whole plant.

"Bakumondou: Bakumondo": Liliacaceae (Liliac)
eae), the root of a plant of the genus Ophiopogon (Ophiopogon japonicus Ker-Gawler or other similar plants).

It is to be noted that it is not known at all that the plant extracts of sesame, sanyak, capsicum, syllium, syllium, dokudami and Bakumondou used in the present invention have a fibroblast proliferation promoting effect.

[0015]

BEST MODE FOR CARRYING OUT THE INVENTION The extracts of sesame, sanyak, capsicum, syllium, dokudami and Bakumondou used in the present invention are various parts of each plant (whole plant, seed, fruit, root bark, rhizome, root) Etc.) and then crushed and exploited. Alternatively, it is extracted with a solvent as it is or after pulverization.

As the extraction solvent, water, ethanol, anhydrous ethanol, methanol, 1,3-butylene glycol, propylene glycol, hexylene glycol, n-butanol, acetone, chloroform, ethyl acetate, n-hexane and the like can be used. Any one of the solvents or a combination of two or more of them can be used. In addition, it can be used in combination with water, ethanol, anhydrous ethanol, methanol, 1,3-butylene glycol, propylene glycol, hexylene glycol, n-butanol, acetone, chloroform, ethyl acetate, n-hexane extraction, etc. it can.

The obtained extract may be optionally dried, concentrated or diluted depending on the type and form of the applied fibroblast proliferation promoter, skin external preparation and bath preparation, and food and drink. good.

Although the production method is not particularly limited, it may be usually within the range of the boiling point of the solvent at normal temperature to normal pressure. After extraction, filtration or use of an ion exchange resin is carried out.
A solution, paste, gel, or powder may be obtained by decoloring and purifying. In many cases, it can be used as it is, but if necessary, further purification treatment such as deodorization and decolorization may be added as long as the effect is not affected. An activated carbon column or the like may be used, and ordinary means generally applied to the extracted substance may be arbitrarily selected.

The sesame, sanyak, capsicum, pepper, tokudami, dokudami and Bakumondou extract of the present invention can be used as a fibroblast proliferation promoter as it is, and can be further incorporated into skin external preparations, bath preparations, and foods and drinks. Although the amount is not particularly limited, it is slightly different depending on the type of fibroblast proliferation promoter, skin external preparation and bath agent, food and drink, quality, expected effect, but usually 0.0%.
1% by weight or more (hereinafter, represented by weight%), preferably 0.1% by weight
1 to 60% is good. If the amount is less than 0.01%, the effect cannot be expected sufficiently.

The fibroblast proliferation promoter, skin external preparation, bath preparation, food and drink of the present invention may contain, in addition to the above-mentioned essential components, pharmaceuticals, if necessary, within a range not to impair the effects of the present invention. It can be produced by arbitrarily selecting and using components and additives used in pharmaceuticals such as quasi-drugs, cosmetics, foods and beverages, and the like.

(1) Various fats and oils Avocado oil, almond oil, fennel oil, perilla oil,
Olive oil, orange oil, orange luffer oil, sesame oil,
Cocoa butter, chamomile oil, carrot oil, cucumber oil, tallow, tallow fatty acid, kukuinut oil, safflower oil, shea butter, soybean oil, camellia oil, corn oil, rapeseed oil, persic oil, castor oil, cottonseed oil, peanut oil, Turtle oil, mink oil, egg yolk oil, cocoa butter, palm oil, palm kernel oil, mocro, coconut oil, tallow, lard, hydrogenated products of these oils (hardened oil, etc.) and the like.

(2) Waxes Beeswax, carnauba wax, whale wax, lanolin, liquid lanolin, reduced lanolin, hard lanolin, candelilla wax,
Montan wax, shellac wax, etc.

(3) Mineral oil Liquid paraffin, vaseline, paraffin, ozokeride, ceresin, microcrystalline wax, squalene, squalane, pristane, and the like.

(4) Fatty acids Natural fatty acids such as lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, 12-hydroxystearic acid, undecylenic acid, tall oil, lanolin fatty acid, isononanoic acid, caproic acid , 2-ethylbutanoic acid, isopentanoic acid, 2-methylpentanoic acid, 2
-Synthetic fatty acids such as ethylhexanoic acid and isopentanoic acid.

(5) Alcohols Ethanol, isopyropanol, lauryl alcohol,
Natural alcohols such as cetanol, stearyl alcohol, oleyl alcohol, lanolin alcohol, cholesterol, and phytosterol; and synthetic alcohols such as 2-hexyldecanol, isostearyl alcohol, and 2-octyldodecanol.

(6) Polyhydric alcohols Ethylene oxide, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, polyethylene glycol, propylene oxide, propylene glycol , Polypropylene glycol, 1,3-butylene glycol, glycerin, pentaerythritol, sorbitol, mannitol and the like.

(7) Esters: Isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleyl oleate, decyl oleate, octyl dodecyl myristate, hexyl decyl dimethyloctanoate, cetyl lactate, Myristyl lactate, diethyl phthalate, dibutyl phthalate, lanolin acetate, ethylene glycol monostearate, propylene glycol monostearate, propylene glycol dioleate, and the like.

(8) Metal soaps Aluminum stearate, magnesium stearate, zinc stearate, calcium stearate, zinc palmitate, magnesium myristate, zinc laurate, zinc undecylenate and the like.

(9) Gum, saccharides and water-soluble polymer compounds Arabic gum, benzoin gum, dammar gum, guaiac oil, Irish moss, karaya gum, tragacanth gum,
Carob gum, quinseed, agar, casein, lactose,
Fructose, sucrose and its esters, trehalose and its derivatives, dextrin, gelatin, pectin, starch, carrageenan, carboxymethyl chitin or hydroxyalkyl (C ₂ -C ₄ ) oxide to which alkylene (C ₂ -C ₄ ) oxide such as ethylene oxide is added _{2 to} C ₄ ) chitin or chitosan, low molecular weight chitin or chitosan, chitosan salt, sulfated chitin or chitosan, phosphorylated chitin or chitosan, alginic acid and its salt, hyaluronic acid and its salt, chondroitin sulfate and its salt, heparin, ethyl cellulose , Methylcellulose, carboxymethylcellulose, carboxyethylcellulose, sodium carboxyethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, nitrocellulose, crystalline cellulose Polyvinyl alcohol, polyvinyl methyl ether, polyvinyl pyrrolidone, polyvinyl methacrylate, polyacrylic acid salts, polyalkylene oxide or crosslinked polymer thereof such as polyethylene oxide or polypropylene oxide, carboxyvinyl polymers, such as polyethyleneimine.

(10) Surfactant Anionic surfactant (alkyl carboxylate, alkyl sulfonate, alkyl sulfate, alkyl phosphate), cationic surfactant (alkyl amine salt, alkyl quaternary ammonium salt) ), Amphoteric surfactant:
Carboxylic acid type amphoteric surfactant (amino type, betaine type), sulfate ester type amphoteric surfactant, sulfonic acid type amphoteric surfactant, phosphate type amphoteric surfactant, nonionic surfactant (ether type nonionic) Surfactants, ether ester type nonionic surfactants, ester type nonionic surfactants, block polymer type nonionic surfactants, nitrogen-containing nonionic surfactants), other surfactants (natural surfactants) , Derivatives of protein hydrolysates,
Polymer surfactant, surfactant containing titanium and silicon,
Fluorocarbon surfactants).

(11) Various vitamins Vitamin A group: retinol, retinal (vitamin
A₁), Dehydroretinal (vitamin A_Two), Carochi
N, lycopene (provitamin A), vitamin B group: thia
Min hydrochloride, thiamine sulfate (vitamin B₁), Lvov
Rabin (vitamin B_Two ), Pyridoxine (vitamin
B₆), Cyanocobalamin (vitamin B₁₂), Folic acid,
Nicotinic acids, pantothenic acids, biotins, choline,
Inositols, vitamin C group: ascorbic acid and its
Derivatives, vitamin D group: Ergocalciferol (bi
Tamin D_Two), Cholecalciferol (vitamin D_Three),
Dihydrotaxerol, Vitamin E group: Tocophero
And its derivatives, ubiquinones, vitamin K group:
Tonadione (vitamin K₁), Menaquinone (vitamin
K_Two), Menadione (vitamin K_Three), Menadiol (bi
Tamin K_Four), Other essential fatty acids (vitamin F), mosquitoes
Lunitine, ferulic acid, γ-oryzanol, orot
Acids, vitamin Ps (rutin, eriocitrin, hesperi
Gin), vitamin U, etc.

(12) Various amino acids Valine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, serine, cysteine, cystine, tyrosine, proline, hydroxyproline, aspartic acid, glutamic acid , Hydroxylysine, arginine, ornithine, histidine, and the like, and their sulfates, phosphates, nitrates, citrates, and amino acid derivatives such as pyrrolidone carboxylic acid.

(13) Various additives derived from plant or animal raw materials These are usually processed according to the type and form of the product to be added (for example, grinding, milling, washing, hydrolysis, Fermentation, purification, squeezing, extraction, fractionation, filtration, drying, pulverization, granulation, dissolution, sterilization, pH adjustment, deodorization, decolorization, etc. You can choose to serve.

The solvent to be used for the extraction may be selected in consideration of the purpose and type of the product to be provided, and the processing to be performed later. However, usually, water and a water-soluble organic solvent (for example, ethanol) are used. , Propylene glycol, 1,3-butylene glycol, etc.).
However, when the content of the organic solvent is not preferable depending on the application, only water may be used, or ethanol which is easily removed after the extraction may be used, and may be used alone or in an arbitrary mixed solution with water. Exploited extract may be used.

In addition, additives derived from plant or animal raw materials are
When applied to systemic or topical external preparations and cosmetics, including protection of skin and hair, moisturizing, improvement of feel and texture,
Gives flexibility, relieves irritation, relieves stress due to aroma, activates cells (prevents cell aging), suppresses inflammation, improves skin and hair quality, prevents and improves rough skin, hair growth, hair growth, hair loss prevention, gloss Application, cleaning effect, alleviation of fatigue, promotion of blood flow,
In addition to cosmetic effects such as a warm bath effect, effects such as flavoring, deodorizing, thickening, preserving, and buffering can be expected.

In the case of edible products, health and cosmetic effects such as nutritional supplementation, recovery from fatigue, tonicity, cell activation (prevention of cell aging) and the like, improvement of taste, provision of color tone, aroma and gloss, stabilization, It can be used for thickening and preservation. Furthermore, in addition to this, various cosmetic and pharmaceutical effects of each known raw material are expected, and by combining them, the intended effect is enhanced or multi-functional. It is also possible to make a product that expects an effect.

As a specific plant (crude) used as a raw material,
For example, almond (Hen peach), eye (Aiba), blue oak
(Seifu Fuji), Aotsuji Lafzi (Kibo), Acacia, Akasuri Fruit, Red Grape, Red Megawiwa (Red Bud), Akane
(Kanekane Akane), Ayajiou Jiou (Jihuang), Agi (Awei),
Akinire (Royu skin), Akebi (Kidori), Asa (John Asako), Maruba Asagao or Asagao (Toshiko), Ashitaba (Asuka),
Azuki (red azuki), acerola, ase yak (Asenyaku), anise, Abemaki fruit, avocado, ama, amacha (sweet tea),
Amachaworu, Amadokoro (Tamatake), Amigasa lily (Shellfish), Altea, Arnica, Aloe (Length), Aloe vera, Angelica, Anzu Honganzu (Anjin), Ansokkou (Azuka), Ikakozolina (Biliary head), Itadori
(Tiger cane root), strawberry, fig (fructose) or its leaves, strawberry (winter aoiko), ichiyakuso, ginkgo (ginko leaf, ginkgo),
Itohimehagi (Enshi), carob, rice seed or seed coat,
Inondo seeds, Ibuki-kusou-so, nettle, ylang-ylang, fennel (Fenka), ukiyagara (sanryou), gull-kasukagra fruit, Hime-uikyo, turmeric (Utsukin), Usubasaishin-keirinsaishin (spicy), Usveniaoi,
Pterodactyla mushroom, Nepenthes (summer hay), Udo or Shishudo (Qang, Independent, Tang Independent), Plum (Ume) or its pulp, Vladigashi, Uwaurushi, Unshuri mandarin (Chen skin), Estragon, Ezocogi (Ezo fivega), Enoki ,
Ebisugusa (Keiko), Elderberry fruit, Elemi, Enju (Gossip, Gossip rice), Ogi-kibana-ohgi (Koki),
Ouren (yellow), giant crow squirrel (karonin), giant skull (self-defense), psyllium (vegetative child, vegetative grass), toucan hazel (Haruko), obanokera okera (white jitsu), obayashishabushi fruit, o hawthorn hawthorn ), Barley (barley), oguruma (turning over), okra fruit, Panax ginseng and horseradish (carrots), Hypericum persicae, Kogomebayotogirisou (Brother's cutting), Odikosou (interrupted), Onamimomi
(Aoiko), Onigurumi, Oninoyagara (Tianma), Onyuri Sasasayuri Hakatayuri (Yuri), Ononis, Halibut
(Hihayu), Ominaeshi (losing sauce), Dutch strawberry, Dutch mustard (Watercress), Dutch jelly, Dutch honeybee,
Olive, oregano, orange fruit or peel, silkworm (ripe yellow), cacao seed, oyster or its leaves (persimmon),
Kagikazura (Hook of Choto), Cassia, Cassinaki fruit (Kozomi), Gajutsu (Shazajutsu), Kashiwa (Kazuki), Cascarilla, Kanixa (Kinosato), Kanokosou (Kikusone), Birch or Shirakaba (Shirakaba) , Squash, capoknoki seeds, kahokusansho (shopper), cattail (bamboo), chamomile roman chamomile, kamiyatsude (weeding), crow squirrel (golden melon), rasabisaku (midsummer), oats, guarana seeds, karaya, karin ( Gourd), Kawayanagi, Kawataketake, Kawarayogi (Inchinko), Kansui (Sweet), Licorice (Licenzo), Candelilla, Kantou, Canna, Raspberry, Kiwi fruit, Kikarasuuri (Ururone), Kikyo (Kikyo, Bellflower root) ), Chrysanthemum (chrysanthemum), catalpa (Azumi), sagebrush (yotei), pheasant (kotomi), kizuta, kidachi aloe, edible mint, chinoki, edible (yellow), gimnema sylvestre, edible Jabec,
Cabbage immature fruit, cucumber, Kiraja saponaria, Kiraya, kumquat fruit, betel, kinmizukiki (senkaku grass),
Guava fruit, guayule, Quercus infectria (gallicia), cucumber nuts, wolfberry (Gugi, gugi, gugi leaf, skeletal bark), cedar spruce (Tenmen winter), kuzu (kuzukane),
Camphor tree, gooseberry fruit, gardenia, gardenia, blackberry, kumazasa, black pine, horseshoe
(Cranberry), cranberry fruit, chestnut or its astringent skin, curculigo-latifolia fruit, grapefruit fruit, buckthorn, black crocodile (rescue), clove (clove,
Dingka), Kaigai (Epispermia japonica), Celosia (cow flower, Chicken corolla), Bayberry (laurel), Gentiana, Gennoshoko (Old grass), Koshinbara (Monthly flower), Kosuihakka (Melissa), Kozo fruit, Kocha (tea), Kouhone
(River bone), kouhon (straw and kara straw), koulyang, koendro fruit, koganebana (yellow gon), lingonberry (koshibachi), coconut fruit, goshyu (goshuyu), goshoichigo (covered bonsai),
Pepper (pepper), copaiba balsam, coffee beans, kobushi tamshiba magnolia (hot spring), burdock (burdock, burdock), kobotdur, sesame clover (gensen), wheat (wheat), rice / rice bran (red bran, white bran)・ Rice oil, cola acuminata seed, cola vera seed, fenugreek fruit, colombo,
Konzulango, Kombu, Konjac, Comfrey (fin-bared grass), Sakisima button (Eureisen), Sakura leaves / flowers /
Fruit / bark (cherry bark), cherry, pomegranate, sasa, lily of the valley, lily of the valley, sasanqua, sasamodaka (sawashatsu), sweet potato, sugar cane, sugar beet, jujube (jujube), saffron (red safflower, western safflower), pomelo fruit , Saponoso, salmon berry fruit, salicinosa (mashima), salvia (sage), sawagkyo (yamakona), hawthorn (yamazako), sanshin ginseng (seven carrots), sanshuyu (sanshuyu), sansho (sansho) ), Sandscon (Yamato root),
Shea (carite), cyanoki fruit, shiitake (shiitake), shion
(Shiwon), Red squirrel, Shikunshi (Kimiko), Perilla, Aojiso, Chirimenjiso, Catamendiso (Shiyoha, Shiso), Rosewood, Shinanoki, Chinese forsythia, Lily of the valley
(Chrysanthemum flower), Spiraea, Potato, Peony (Shakuyaku), Shajin (Sasan), Juzudama, Palm fruit, Ginger (Ginger), Shobu (Iris, Iris root), Shozuku fruit,
Shirakashi Seed, Shirogoshi Butterfly Seed, Shirobanairis,
Shirobanatsuta flower, Shirominanten (Namitenmi), Cinchonasakusylbra, Cinnamon, Watermelon (watermelon), Honeysuckle
(Gold and silver flowers, shinto winter), sorrel (acid model), swimberry fruit, strawberry fruit, horsetail (infestation), stevia, plum fruit, Scots pine cone, larch, Scotch pine, Scotch mulberry, Scots pear, Scots dandelion, Horse chestnut (Marronnier), pear fruit, Prunus aegypti, A. var.
Horses, achillea millefolium,
Rose, cornflower, mistletoe, mint, mint, horseradish, sekisho (stone iris), mallow, hirohasenega, senega, senega, seri, celery, senkyu (river cucumber), medicine , Senna fruits or leaves, Sengyousou (daisatsu), Soka (grass), buckwheat seed, Somei potato, Daio (daisuki), radish, soybean, Daidai
(Orange, kikomi), takatodai (daigeki), takarababi (guin), dark sweet cherry fruit, hollyhock, hollyhock thyme (thyme, momoka), hollyhock (tachibana skin), hollyhock willow, tamarind seeds, onion, tamshiba ),
Tara tree or its root bark, tanjin (dansin), dandelion (gakko) or white dandelion and moko dandelion, dammar, cherry fruit, tigaya or its root (chinese), chikutsujin carrot (bamboo ginseng), chicory, ginseng (gomiko) , Korean ginseng (Rhuanghuang), Korean ginseng (Wuyi), Korean ginseng (Ginseng), Korean ginseng (Aiba leaf), Chinese ginseng (Irei), Japanese radish, Camellia,
Butterflies, clovers (lacquer primroses), twigs (ducklings), vines (red azuki), vines (red bean), vines, vines (vegetables), vines (four-leaf ginseng), vines, deco, teuchigurumi, duberry fruit, tengusa, tendaiyaku
(Crowd drug), touga (winter gourd), calendula (marigold), corn or corn hair (nanban hair), horned rattle (woman Sadako), tourindo (dragon gall), tokkon
(Root), carrot (bamboo ginseng), eucommia (mushroom,
Tochu, Tomato, Tragant, Triacanthus seed,
Tormentilla, Dronoki, Troloa oyster, Niseria berry fruits, Ragwort (Butcher Bloom), Naginata Kouju, Nazuna, Rape, Natsumikan, Jujube (Daijutsu), Naniwaibara (Kanekurako), Narco Lily (Yoshisei), Peanut (Peanut), Nanten (Nanten) Minami Tenmi), Nigaki (Maki),
Japanese sagebrush (bitter mite), nutmeg, nikkei (cinnamon), chive
(Niriko), fruit / flower or foliage of elderberry (bone-attached tree), garlic (garlic), nulde (fivefold child), leeks, thistle (daiji), wild rose (real fruit), saw millet, sawdust (mae hu), nobara,
Nomomo, palm, pineapple fruit, hibiscus (Bussouge, Furinbussouge, Roselle), Hakata lily (Yuri), Hakamauraboshi (bone crushing), Hakusen (Rinosa rind), Huckleberry fruit, Hakobe (Horiko), Hashibami (Haruko), Hashidokoro ( Roth root), basil, lotus (lotus, lotus root), parsley (holland jelly), naked mussels, batata, hachiku madake (bamboo boiled), patchouli, mint (lightly loaded, lightly loaded leaves), adlay (yokuinin), hanasuge (knowledge) Mother), banana, honey mint, vanilla bins, papaya, japonicus
(Rabbit malt grass), Habu grass, Paprika, Hamago / Mitsubahamago (vine thorns), Hamasuge (Kazukiko), Hamabishi (Situriko),
Hamanas (maikai flower), Hamabofu (beach storm), hamamelis, rose (rose), Parietaria, harunire (yubari, yubaishi, yuba), breadfruit, hioogi (sun-dried), lycopodium carrot (partisan), pecan nut, ganganbana (Ishiru,
Jinshasha, Hiokikoshi (prolonged life grass), Hishi (Hisami), Pistachio, Beet, Hitotsuba (Ishire), Hinata Kozuchi (cow knee), Hinoki, Hiba, Castor, Sunflower, Bell pepper, Himegama Himematsutake, Pimenta fruit, Juniper tree, Hydrangea fruit, Beroud oyster, Hiroha okinagusa (Hakutooka), Loquat (Loquat, Loquat leaf), Betel wax (Large garment, Betelnut leaf), Houtou Kazura (Southern rattan) Subsection winter leaves), Fujibakama (orchid grass), Fuji beans (bean beans), grape fruit or pericarp / leaves, beech, fuyushinatsukutaketake
(Cordyceps), Brazilian liquorice, black currant fruit, blackberry, plum fruit, flucellaria, blueberry (Acacia catechu), prunes, blonde psyllium, bundou (green beans), loofah, safflower (safflower),
Belladonna, berry fruit, boysenberry fruit, boufu (windbreak), spinach, physalis (Torone), honoki
(Park, Wakkopoku), Bokeh (Gourd), Hosobanaokera (Sojutsu),
Hosobabarengiku, Bodaiju (bodai tree), button (peony, peony bark), hop, jojoba, white berry fruit, hon-shimeji, meizurutennanshaw (Tenan star), ephedra (maoh), macadamia nut, macri (seaweed), maguwa (Mulberry white skin, Mulberry leaves), Madake (Bamboo boiled), Matabi (Kiten Tate),
Matsukasa, Matsuba, Matsuhodo (Bukuryo), Mayorum (Hanamint), Malvanozadin (Bitterfruit), Mulberry Fruit, Quince, Malonie, Mango, Mangosteen, Mansugurumi, Mandarin Fruit, Mannentake (Lingzhi), Fruits of Citrus Plants (Komimi) , Mishimasaiko (Saiko), Mizokokushi
(Hanabe lotus), Misohagi (Chisatsuna), Mitsugashiwa, Mitsuba, Mitsubahamago (vine stalks), Midorihakka, Mimosa, Myoga, Miracle fruit fruits, Myrrh, Myrobaran, Mukuge (Mokugeki), Mukunoki, Mukuroji, Murasaki ( Purple roots), purple corn, sorghum (meadow grass), mebaugi, melaleuca, melissa, melilot, melon fruit, sagebrush, mosouchikku, mokkou (wood incense), maple blossom, peach leaf (peach leaf), fruit, seed (peach kernel), Bean sprouts, morello cherries, moroheiya (burlap), yakawamurasakiimo, yakuchi (masuchi), cornflower mackerel (cornflower), cornflower mint, yashabushi (arrow wheel), yayanagi, yatsude
(Octagonal gold plate), mistletoe (parasitic willow), willow leaf, yabugarashi, yabukouji (purple gold beef), pokeweed (commercial land), yamahanoki (yamaharu), yamamomo (yangmei), yamakomogi, eucalyptus, yukinoshita (tiger ear) Grass), Yucca flebifolia, Yuzu fruit, lily, yogurusa, mugwort (mushroom leaf), lime fruit, rye, lacanca fruit, raspberry fruit, lavender, longan (longan meat), ryocha (green tea), apple fruit, gentian, rubus suavisimus (Sweet beet), lettuce, red currant fruit, lemon fruit, lemongrass, forsythia (forsythia), astragalus, wax palm, loganberry fruit, logwood, rosemary (mannenrow), rosehip (nobara), wakame, wasabi, Waremokou (chiyu) and the like.

Examples of the seaweeds include seaweeds [green algae: Chlorella vulgaris, Chlorella pyrenoidosa, Chlorella ellipsoidia, Usubaaonori, Sujiahonori, Hiraaonori, Bouuaonori, Hosoedaoonori], and seaweeds [Brown algae: Macombum, Kosomebu, Hoshimesobu, Hosoedohn] Honey kelp, wakame, hirome, blue seaweed, macrocystis pilifera, macrocystis integrifolia, neocitys luetokeana, hijiki, hibamata], seaweed [red algae: red alga: jijirimen, maxa (tengusa), hirakusa, onyxa, obaxa, kataobaxa ,
Yatabegusa, Yuikiri, Shimatengusa, Tosakanori, Togekirinsai, Akumasakirinsai, Kirinsai, Byaksinkirinsai, Tsunomata, Obatsunomata, Tochaka (Yahatsunomatata), Ezotunomata, Togetsunomata, Hirakotonogi, Kotoji, Kotoji , Heius Banori] and the like.

Other algae, for example, green algae (Chlamydomonas: Chlamydomonas, Akayukimo, Dunaliella: Dunaliella, Chlorococcus: Chlorococcus, Kwanomimo: Kwanomimo, Volvox: Ohikawawari, Volvox, Palmera, Yotsumemo, Spirogyra: Hizaori, Spirogyra, Turugimidro, Hibimidoro: Hibimidoro, Aosa: Anaoasa, Amiosa, Nagaosa, Kawanori: Kawanori,
Genus Fricciella, genus genus: medusa, asamidori ogusa, kawashiogusa, marimo, valonia: tamagobaronia, tamabaronia, magnum: tamatamamo, iwata: husaiwata, suricogita, heraiwirata, crocodile, crocus Sakibutimimil, Nagamil, Hiramil, and Casanori:
Casa nori, Juzumo
Species of the genus Mizudzumo, Genus genus, Colecaetus, Genus genus genus, Species of the genus Rosa: Rosa spp. Genus (Suizenjinori: Suizeninori, Aokko, Nostoc: Kawatake, Ishikurage, Hassai, Yuremo, Racenmo (Spirulina): Spirulina,
Genus trichodesmium (Aiakashio)), brown algae (Piraella spp .: Pyraella spp., Shiomidoro spp .: Nagamioshidoro, Isogrape spp .: Isogrape, Isogawara spp .: Isogawara, Krogashira spp .: Gunsenkurogashira, Kasilaki spp .: Kashirazaki, Muchimo spp .: Muchimo, Hiramchimo, Kebelligsa, Amidixa: Amidixa, Sakibiroamiji,
Sanadagusa: Sanadagusa, Fukurinamiji, Commongusa: Commongusa, Yahuzugusa: Ezoyahazu, Yahuzugusa, Uraboshiyahazu, Jingamigusa: Jigamigusa, Umiuchiwa: Genus Umiuchiwa, Konamiumichusawa, Akabaumikawasuna Genus Nabarimo, Chasoumen spp. , Mozuku: Mozuku, Ishige: Ishige, Irolo, Icmegasa: Ichimegasa, Carapacea: Callidia, Umibos: Umibos, Urushigusa: Urushi Sa, Keurushigusa, Tabakogusa, Konbumodoki genera: Konbumodoki, Habamodoki genera:
Genus Habanomoki, Genus Habanori: Havanori, Genus Habanori: Habanori, Common Bukuro: Common Bukuro, Ezobukuro: Ezobukuro, Fukuronori: Fukuronori, Watamo, Chishimafukuronori Genus: Genus Genus, Kagomenorimura, Genus Kamuramemura ： Genus Shark, Genus Genus: Genus Genus, Genus Genus: Genus Genus, Genus Genus: Genus Lily, Genus Genus: Genus Genus, Genus Genus:
Turmo, Anatome: Aname, Siberium: Sujime, Misujikombu: Misjikombu, Atsubamisujikombu, Kombu: Gatsugarakomubu, Oysterfish, Onicombu, Goheikombu, Nagakombu, Pea, Ootomikombu, Torokomokuto: Torokontokuto , Genus genus: swordfish, swordfish, black squid, genus scorpionum: squirrel, squirrel genus: squirrelcomb, kuroshiome: kuroshiome, scorpion genus: feline sacion, alame genus: alame, Ainuokame: Ainuwakame, squid, squid Ezoishige, Yabamoku: Yabamoku, Rappermoku: Rappermoku, Jiromoku: Uganomok,
Joromok, Hyemok, Hondawara: Tamanashimoku,
Isomok, Nagashimamoku, Akamoku, Shidamoku, Hondawara, Nejimoku, Narasamo, Mametara, Tatsukuri, Yasumatamoku, Umitranoo, Oobamoku, Fushizushimoku, Hahakimoku, Togemoku, Jolemoku, Sawtooth moku,
Obanokogirimoku, Sugimoku: Sugimoku, Ukimo: Oukimo, Buruquimo: Burukimo, Kayamonoli: Kayamonori, etc., Red algae (Ushikenori: Ushikenori, Funorinoushike, Amanori: Asakusanori,
Susabinori, Uppluinori, Onimanori, Tasa, Filitasa, Benitasa, Rhodocorton spp. Pine genus:
Pine L., Fusanori: Fusanori, Nisefusanori: Nisefusanori, Sodegarami: Sodegarami, rattle:
Rattle rattle, rattle rattle, broad-spotted genus: broad-spotted scorpion, scorpion genus: small-spotted squirrel, genus Kagikenori: Kaginori, Kakenenori, Tengusa genus: Himetengusa, Hytengusa, Oobusa, Nambugusa, Kohido, Kohira, Kojira, Ohihime, Kohido, Kouhira, Ohihime ： Genus genus: genus genus: genus genus: genus ryegrass, genus ryumunsou, genus rhyme, genus rhinoceros, genus nisekarekigusa genus: genus foxes, genus okitsubara: genus syllabus, genus akaba: genus akaba, malvagana, sagogana : Genus Shiogosagoromo, Genus Iwanoka: Genus Etsukiiwanokawa, Genus Kinokawa: Genus Kinokawa, Genus Kaninote: Genus Kaninote, Coral: Coral genus, Mucadenori: Mucadenori, Suzukade, Catanori, Hiramukade, Kyounohimo, Sakuranori, Nikumukade, Tambanori, Crane, Isonohana: Nuraksa, Cronulaxa, Omodenori, Hirakintoki: Hirakintoki
Genus: scorpionfish, genus: serrata,
Kintoki, Matsunori, Komenori, Tosukamatsu, Atsumatsu, Kakurite spp. Genus: genus genus: genus genus: quinuhada; Species of Susqueke Beni: Species of Suska Kebeni, Species of Okamuragusa: Yamadagusa, genus of Mirin: Mirin,
Hosobamilin, Tosakanori: Kikutosaka, Ezonameshi: Ezonameshi, Isomokka: Isomokka, Yukari: Yukari, Hosoyukari, Ibaranori: Ibaranori,
Rhinoceros rose, Tachiibara, Kagibaranori, Kijinoo genus: Chidinooo, Isodanthus: Isodanthu, Atsubanori genus: Atsubanori, Ogonori: Ogonori, Tsurushiramo, Silamo, Ogonori, Mizokonori, Kabanori,
Genus genus: beetle scrophulari, fushikrenori genus: fushikrenori, namitake mushroom: namiitake mushroom, sponge genus: sponge beetle, okinori genus: okitsunori, saimi genus: itanigusa, simi, harugane,
Lotus spp .: Lotus spp.
Hosoibonori, Nobonori, Scrophulariaceae: Kurohaginnansou, Akabaginansou: Akabaginnansou, Hishibukuro: Hishibukuro, Madaragusa: Togemadara, Ekimadara, Taoyanagisou: Hanagusa ： Hanaedae, Hirata goats ：
Hirata goat, genus Darus: dars, masagoshibari, anadarus, eubagusa genus: eubagusa, Benifukuronori genus: Benifukukuronori, fushitsunagi: fushitsunagi, himefushinushigi, hirohafushinagi, kawanatsugisugisai, genus tsutsugigigi , Honey Negis, Amixa, Egonori: Egonori, Futigis, Saeda: Saeda,
Chrysanthemum spp .: Chrysanthemum spp. Genus: Aristolochia, Ayanishi, Genus: Ayanishi,
Genus Ayaginu: Genus Ayaginu, Genus Dadia: Enashidazia, Genus Shimadada: Isohagi, Shimadadia, Genus Dazimodoki: Dazimomodoki, Itogusa: Moletogusa, Futigusa, Macri Genus: Makri, Sanaginori: Hanayanagi, Yuna, Yanaginori, Motsunagi
Sasabayanaginori, genus Sozo: Closozo, Kobusoso, Hanesozo, Sozonohana, Hanegusa: Hanegusa, Kehanegusa, Kozanemo genus: Kozanemo, Isomurasaki, Hosokozanemo, Himegoke genus: Himegoke, Kurohimegoshi, Hioudoogushigu , Genus genus: genus cypress, genus genus: genus isola, genus genus: genus genus: genus genus, genus syllabus: genus sylvestris, genus sculpture: genus sculpture: genus genus, genus genus, and : Chinolimo, Chisinori: Chisinori, etc., and axle algae (Shajikumo, Shiratamamo, Hossiturimo: Hossiturimo, Lichnotatum, Fussurimo) Rascomo genus: Himejimo, Chabo hiramo, Tripera, etc.), Yellow algae (Hikarimo genus: Hikarimo etc.) and the like.

Materials derived from animal raw materials include a cockscomb extract, a cow / human placenta extract, a pig / cattle stomach, a duodenum or an intestinal extract or a decomposition product thereof, and a pig / cattle spleen extract. Products or their degradation products, extracts of swine or bovine brain tissue, water-soluble collagen, collagen derivatives such as acylated collagen, collagen hydrolysates, elastin, elastin hydrolysates, water-soluble elastin derivatives, keratin and its decomposition products Or a derivative thereof, a silk protein and its degradation product or a derivative thereof, a pig / bovine blood cell protein degradation product (globin peptide), a pig / bovine hemoglobin degradation product (hemin, hematin, heme, protohem, heme iron, etc.), milk , Casein and its decomposition products or their derivatives, skim milk powder and its decomposition products or their derivatives, lactoferrin or its decomposition products, chicken egg ingredients Such as fish meat decomposition products.

(14) Microbial culture metabolites Yeast metabolites, yeast extract, rice ferment extract, rice bran ferment extract, euglena extract, lactic acid fermentation product of skim milk powder, trehalose or derivatives thereof.

(15) α-hydroxy acid Glycolic acid, citric acid, malic acid, tartaric acid, lactic acid and the like.

(16) Inorganic pigments Silicic anhydride, magnesium silicate, talc, kaolin,
Bentonite, mica, titanium mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide,
Titanium oxide, calcium carbonate, magnesium carbonate, yellow iron oxide, red iron oxide, black iron oxide, gunjou, chromium oxide,
Chromium hydroxide, carbon black, calamine, etc.

(17) Ultraviolet absorbing / blocking agent p-aminobenzoic acid derivative, salicylic acid derivative, anthranilic acid derivative, coumarin derivative, amino acid compound,
Benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives, dioxane derivatives, camphor derivatives, furan derivatives, pyrone derivatives,
Nucleic acid derivatives, allantoin derivatives, nicotinic acid derivatives,
Vitamin B ₆ derivatives, benzophenones, oxybenzone, arbutin, guaiazulene, shikonin, baicalin, baicalein, berberine, neoheliopan, urocanic acid derivatives, escalol, zinc oxide, talc, kaolin and the like.

(18) Whitening agent p-aminobenzoic acid derivative, salicylic acid derivative, anthranilic acid derivative, coumarin derivative, amino acid compound,
Benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives, dioxane derivatives, camphor derivatives, furan derivatives, pyrone derivatives,
Nucleic acid derivatives, allantoin derivatives, nicotinic acid derivatives,
Vitamin B ₆ derivatives, oxybenzone, benzophenone, arbutin, guaiazulene, shikonin, baicalin, baicalein, berberine and the like.

(19) Trosinase activity inhibitor Ascorbic acid and its derivatives, hydroquinone and its glycosides, kojic acid and its derivatives, placental extract, silk peptide, plant extract (mulberry, corn, waremokou, clara, mugwort, honeysuckle, Yellowfin, Dokudami, Matsuhodo, Admiral, Odorikosou, Hop, Hawthorn, Eucalyptus, Yarrow, Althea, Calypt, Mankeishi, Hamamelis, Yamaguwa, Enmeisou, Kikyo, Toshishi, Tsutsukoko, Shako, Mao, Sengoku, Dokkatsu, Psycho, (Boufu, Hamabofu, Ogon, Peony, Peony, Gennoshoko, Kakkon, Licorice, Gobiko, Aloe, Ginger, Safflower, Green Tea, Tea, Asenyaku etc.)

(20) Reduction / decomposition of melanin pigment Hydroquinone monobenzyl ether, phenylmercury hexachlorophene, mercuric oxide, mercurous chloride, hydrogen peroxide, zinc peroxide, etc.

(21) Acceleration of turnover / cell activation Hydroquinone, lactic acid bacteria extract, placenta extract, vitamin A, vitamin E, allantoin, spleen extract, thymus extract, yeast extract, fermented milk extract, etc.

(22) Astringent succinic acid, allantoin, zinc chloride, zinc sulfate, zinc oxide, calamine, zinc p-phenolsulfonate, potassium aluminum sulfate, resorcinol, ferric chloride, tannic acid (including catechin compound) Such.

(23) Active oxygen production inhibitor SOD, catalase, glutathione peroxidase, etc.

(24) Antioxidants ascorbic acid and its salts, stearic acid esters, tocopherol and its ester derivatives, nordihydroguaseletenic acid, butylhydroxytoluene (BHT),
Butylhydroxyanisole (BHA), hydroxytyrosol, parahydroxyanisole, propyl gallate, sesamol, sesamolin, gossypol and the like.

(25) Lipid peroxide production inhibitor β-carotene, plant extract (cultured sesame cells, amateur,
Hypericum, Hamamelis, Clove, Melissa, Emmeiso, Birch, Sage, Rosemary, Minami Minami,
Kina, age, ginkgo, green tea, etc.)

(26) Anti-inflammatory agent Ictamole, indomethacin, kaolin, salicylic acid, sodium salicylate, methyl salicylate, acetylsalicylic acid, diphenhydramine hydrochloride, d or dl-
Camphor, hydrocortisone, guaiazulene, camazulene, chlorpheniramine maleate, glycyrrhizic acid and its salts, glycyrrhetinic acid and its salts and the like.

(27) Disinfectant / Disinfectant Acrinol, sulfur, benzalkonium chloride, benzethonium chloride, methylrosaniline chloride, cresol, calcium gluconate, chlorhexidine gluconate, sulfamine, mercurochrome, lactoferrin or a hydrolyzate thereof.

(28) Humidifier Glycerin, propylene glycol, 1,3-butylene glycol, hyaluronic acid and its salts, polyethylene glycol, chondroitin sulfate and its salts, water-soluble chitin or chitosan derivatives, sodium lactate and the like.

(29) Agents for hair Selenium disulfide, alkylisoquinolinium bromide solution, zinc pyrithione, biphenamine, thianthol, castal tincture, ginger tincture, capsicum tincture, quinine hydrochloride, strong aqueous ammonia, potassium bromate, bromate Sodium, thioglycolic acid, etc.

(30) Flavor Natural animal flavors such as musk, civet, castorium, ambergris, anise essential oil, angelica essential oil, ylang-ylang essential oil, iris essential oil, fennel essential oil, orange essential oil, cananga essential oil, caraway essential oil, cardamom essential oil, Guayakwood essential oil, cumin essential oil, black letter essential oil, cinnamon essential oil, cinnamon essential oil, geranium essential oil, copaiba balsam essential oil, coriandel essential oil, perilla essential oil, cedarwood essential oil, citronella essential oil, jasmine essential oil, gingergrass essential oil, cedar essential oil, spearmint essential oil, western Mentha essential oil, Great Fengxiang essential oil, Tuberose essential oil, T-shaped essential oil, Orange flower essential oil, Winter green essential oil, Truval sam essential oil, Batturi essential oil, Rose essential oil, Palmarosa essential oil, Cypress essential oil, Hiba essential oil,
Sandalwood essential oil, Petitgren essential oil, Bay essential oil, Vetiva essential oil, Bergamot essential oil, Peruvian balsam essential oil, Boad rose essential oil, Camphor essential oil, Mandarin essential oil, Eucalyptus essential oil, Lime essential oil, Lavender essential oil, Linaloe essential oil, Lemongrass essential oil, Lemon essential oil, Rosemary Vegetable flavors such as essential oils and Japanese peppermint essential oils, and other synthetic flavors.

(31) Dyes / colorants Red cabbage dye, red rice dye, madder dye, anato dye, squid dye, turmeric dye, ende dye, krill dye, persimmon dye, caramel, gold, silver, gardenia dye, corn dye , Onion pigment, tamarind pigment, spirulina pigment, buckwheat whole plant pigment, cherry pigment, laver pigment, hibiscus pigment, grape juice pigment, marigold pigment, purple potato pigment, purple yam pigment, lac pigment, rutin, etc.

(32) Sweeteners Sugar, sweet tea, fructose, arabinose, galactose, xylose, mannose, maltose, honey, glucose, miraculin, monelin, licorice extract and the like.

(33) Nutrition enhancer Shell calcined calcium, cyanocorabamine, yeast, wheat germ, egg yolk powder, hemicellulose, heme iron and the like.

(34) Dairy products Milk, cheese, fresh cream, butter, margarine, powdered milk, whey, condensed milk and the like.

In addition, humectants, hormones, sequestering agents, pH adjusters, chelating agents, antiseptic / antibacterial agents, cooling agents, stabilizers, emulsifiers, animal and vegetable proteins and their decomposed products, animal and vegetable Vegetable polysaccharides and their degradation products, animal and vegetable glycoproteins and their degradation products, blood flow enhancers, anti-inflammatory and anti-allergic agents, cell activators, keratolytic agents, wound healing agents, foam enhancers, thickeners Agents, oral preparations, deodorants / deodorants, bitters, seasonings, enzymes, and the like can be used, and additive and synergistic effects can be expected by using them in combination.

The fibroblast proliferation-promoting agent of the present invention, the external preparation for the skin, the bath preparation, and the food and drink can be in any dosage form, such as ampules, capsules, powders, granules, pills, and tablets. , Pharmaceuticals such as solid, liquid, gel, foam, emulsion, cream, ointment, sheet, etc., quasi-drugs,
It can be used by blending it into cosmetics for skin and hair, bath preparations and foods and drinks.

Specifically, for example, pharmaceutical preparations for internal and external use, basic cosmetics such as lotions, emulsions, creams, ointments, lotions, oils, packs, facial cleansers and skin cleansers, shampoos, rinses, and hair treatments , Hair creams, pomades, hair sprays, hair styling products, perm agents, hair tonics, hair dyes, hair growth and hair growth products, and other hair cosmetics
Makeup cosmetics such as foundation, white powder, whitening, lipstick, blusher, eyeshadow, eyeliner, mascara, eyebrows, eyelashes, etc.
Includes perfumes, bath agents, other toothpastes, mouth fresheners / gargles, liquid odor / deodorant inhibitors, sanitary products, sanitary cotton, wet tissues, etc., and also used in general foods and drinks It is possible.

The method of adding to the sesame, sanyak, capsicum, cucumber, dokudami, and baked monster extract fibroblast growth promoters, skin external preparations and bath preparations, and foods and drinks of the present invention is added in advance. However, it may be added during the production, and may be appropriately selected in consideration of workability.

[0066]

EXAMPLES The present invention will now be described with reference to production examples, test examples, and prescription examples, but the present invention is not limited to these examples.

(Production Example 1) 100 g of each of sesame, sanyak, capsicum, corn, dokudami, and baked montou was heated and extracted with purified water (about 80 ° C.) for about 5 hours, filtered and extracted (dry solid content: About 0.1 to 20.0% by weight).

(Production Example 2) 100 g of each of sesame, sanyak, capsicum, touki, dokudami and bakmondou
Immerse in 50% ethanol solution or 30% 1,3-butylene glycol solution, or 60% propylene glycol solution, or purified water (about 30 ° C), extract at room temperature for 5 days and nights, filter and extract (dry) Solid content: about 0.1 to 20.0% by weight)
kg.

(Test 1) Fibroblast proliferation promotion test The present inventor used MTT based on normal fibroblasts of the skin.
Using the reduction method, the effect of promoting cell growth was tested and evaluated.

(Test Method) The MTT reduction method is a cytotoxicity and cell activation evaluation method (rapid color quantification of growing and viable cells: Tim Mosmann; Journal of Immunological Met)
hods p55-63 (1983)) and MTT (3- (4,
5-dimethyl-2-thiazolyl) -2,5-diphenyltetrazolium br
omide) is taken up by cells, is reduced and cleaved by NADH present in mitochondria in the cells, and utilizes the property of producing water-insoluble blue formazan.This blue formazan is dissolved in acidic isopropanol, and the absorbance is measured. It is to be measured. That is, since the amount of blue formazan is expressed as the absolute amount of mitochondria, the amount of blue formazan that changes over time was measured, and the cell proliferation rate was calculated.

(Quantification of Fibroblast Proliferation-Promoting Effect) The present inventor has proposed that FBS in which cells do not grow or decrease in advance.
(Fetal calf serum) The concentration was examined and MEM containing 0.5% FBS
It was confirmed that this condition was satisfied when cultured in a medium, and the serum concentration of the test medium was adjusted to 0.5%, and it was decided that the fibroblast proliferation promoting effect of only the test sample could be quantitatively evaluated.

(Measurement of fibroblast proliferation promoting action) 5% F
Using a MEM medium containing BS (fetal calf serum), human dermal fibroblasts (NB1RGB purchased from RIKEN Genebank / Cell Development Bank) at a density of 5 × 10 ³ Cell / Well for 96 Wells pl
ate and cultured for 24 hours under the conditions of 5% CO ₂ and 37 ° C.
Thereafter, the sample was replaced with a MEM medium containing 0.5% FBS to which samples of each concentration (Production Example 1: various plant extracts) were added. At this time, the blank was a MEM containing 0.5% FBS containing no sample.
Change the medium to the same medium every 3 days for 9 days
Culture was continued in a CO ₂ incubator, and the absorbance difference between 570 nm and 620 nm was measured by the MTT reduction method. At this time, the absorbance difference of the blank was set to 100, and the cell growth rate of the test sample-added group with respect to the difference was measured.

[0073]

FIG.

[0074]

FIG. 2

(Test Results) As shown in FIGS. 1-2, it was confirmed that the extract of sesame, sanyak, capsicum, toucan, tokudami, dokudami and Bakumondou of the present invention has a fibroblast proliferation promoting effect.

(Test 2) Safety test (1) Primary skin irritation test Various plant extracts obtained in Production Examples 1 and 2 were prepared in purified water so as to have a dry solid concentration of about 0.5% by weight. Japanese rabbits with female back shaved (female, 3 per group, weight 2.3kg)
Before and after). The judgment was made 24, 48, and 72 hours after application by the primary irritation scoring method using erythema and edema as indices. The result was negative in all animals without any erythema and edema.

(Test 3) Safety test (2) Cumulative skin irritation test Similarly, various plant extracts obtained in Production Examples 1 and 2 were added to purified water so that the dry solid concentration was about 0.5% by weight. Guinea pigs (female,
Once a day, 5 times a week on the skin of 3 animals per group, weight around 320g)
0.5 ml / animal was applied. Application was performed over two weeks and shaving was performed on the last application day of each week. Judgment was made on the day of each application and on the day after the last application using the primary irritancy scoring method using erythema and edema as indices. As a result, all animals were judged as negative without any erythema and edema over 2 weeks.

(Test 4) Safety test (3) Acute toxicity test Similarly, various plant extracts obtained in Production Example 1 were concentrated under reduced pressure.
The dried powder was fasted for 4 hours before the test.
2,000m in mice (male and female, 5 mice per group, 5 weeks old)
g / kg was orally administered, and the appearance and degree of toxicity symptoms were observed over time. As a result, no abnormalities were observed in all the mice for 14 days, and no abnormalities were observed in the dissection results. Therefore, the LD ₅₀ was determined to be 2,000 mg / kg or more.

(Prescription examples) Production of various fibroblast cell growth agents, skin external preparations and bath preparations The following formulation examples are shown below according to the above evaluation results, and each formulation example is produced by a conventional method in the production of each product. And only the amount was shown. Further, the present invention is not limited to these.

(Formulation Example 1) Emulsion weight% 1. Squalane 5.0 2. Olive oil 5.0 3. Jojoba oil 5.0 4. Cetyl alcohol 1.5 5. Glycerin monostearate 2.0 6. Polyoxyethylene (20) cetyl ether 3.0 7. Poly Oxyethylene (20) soorbitan monooleate 2.0 8.1,3-butylene glycol 1.0 9. Glycerin 2.0 10. A: Sesame hot water extract B: Sanyak 20% ethanol extract C: Hot pepper extract D: Touki 30 % 1,3-butylene glycol extract E: dokudami 20% chlorophyll glycol extract F: Bakmondou 20% anhydrous ethanol extract * Extract of any one of AF 5.0 11. Perfume, preservatives qs 12. Water 100 and the rest

(Formulation Example 2) Lotion% by weight 1. Sorbit 2.0 2.1.3-butylene glycol 2.0 3. Polyethylene glycol 1000 1.0 4. Polyoxyethylene oleyl ether (25E.O.) 2.0 5. Ethanol 10.0 6. Sesame heat Water extract 3.0 7.pH adjuster qs 8.preservative qs 9.Purified water 100 residue

(Formulation Example 3) Peel-off pack (% by weight) 1. Glycerin 5.0 2. Propylene glycol 4.0 3. Polyvinyl alcohol 15.0 4. Ethanol 8.0 5. Polyoxyethylene glycol 1.0 6. Sanyak 20% 1,3-butylene glycol extract 4.0 7. Appropriate amount of fragrance and preservative

(Formulation Example 4) Cold cream weight% 1. Beetle wax 11.0 2. Liquid paraffin 22.0 3. Lanolin 10.0 4. Borax 0.5 5. Pepper 30% ethanol extract 2.0 6. Appropriate perfume and preservative 7. Purification Water 100 and the rest

(Formulation Example 5) Shampoo wt% 1. Triethanolamine lauryl sulfate 5.0 2. Sodium polyoxyethylene lauryl ether sulfate 12.0 3.1,3-butylene glycol 4.0 4. Diethanolamide laurate 2.0 5. Disodium edetate 0.1 6. Hot water extract of Touki 5.0 7. Appropriate amount of fragrance and preservative 8. Residue to 100 purified water

(Formulation Example 6) Body soap weight% 1. Potassium laurate 15.0 2. Potassium myristate 5.0 3. Propylene glycol 5.0 4. 30% absolute ethanol extract of Dokudami 7.0 5. Appropriate amount of pH adjuster 6. Appropriate amount of preservative 7. Residual with 100 purified water

(Formulation Example 7) Rinse weight% 1. Stearyltrimethylammonium chloride 2.0 2. Cetostearyl alcohol 2.0 3. Polyoxyethylene lanolin ether 3.0 4. Propylene glycol 5.0 5. Bacmondou hot water extract 4.0 6. pH adjuster Appropriate amount 7.Preservatives Appropriate amount 8.Purified water 100 residue

(Formulation Example 8) Hair liquid weight% 1. Ethanol 29.0 2. Polyoxypropylene butyl ether phosphoric acid 10.0 3. Polyoxypropylene monobutyl ether 5.0 4. Triethanolamine 1.0 5. Sesame hot water extract 2.0 6. Dokudami Hot water extract 3.0 7. Preservative appropriate amount 8. Purified water 100 residue

(Formulation Example 9) Hair tonic weight% 1. Ethanol 40.0 2. Ethyl oleate 1.0 3. Polyoxyethylene (40) hydrogenated castor oil 2.0 4. A: Sesame hot water extract B: Sanyak 20% ethanol extraction Liquid C: Capsicum hot water extract D: Touki 30% 1,3-butylene glycol extract E: Dokudami 20% Fluorene liquid recall extract F: Bacmondou 20% anhydrous ethanol extract * Extraction of any one of AF Liquid 5.0 5. Preservative appropriate amount 6. Purified water 100 residue

(Formulation Example 10) Granule bath agent weight% 1. Sodium bicarbonate 61.0 2. Anhydrous sodium sulfate 35.0 3. Borax 1.0 4. A: Sesame hot water extract B: Sanyak 20% ethanol extract C: Pepper Hot water extract D: Touki 30% 1,3-butylene glycol extract E: Dokudami 20% phylophenolic alcohol extract F: Bakumondou 20% anhydrous ethanol extract * A-F any one extract 3.0

(Formulation Example 11) Coating protective agent A gauze or liniment cloth is impregnated with a prescription liquid prepared by mixing an appropriate amount of a mixed extract of hot water and dokudami with hot water, an antibiotic, an anti-inflammation, and attached to the wound. In addition, the touki extract is directly
It may be sprayed locally and covered with gauze or the like.

(Formulation Example 12) Juice drink weight% 1. Glucose liquid sugar 33.0 2. Grapefruit juice 60.0 3. Fragrance 1.0 4. Dokudami hot water extraction powder 3.0 5. Bakumondou hot water extraction powder 3.0 6. Acidulant proper amount

(Formulation Example 13) Mentsuyu liquid% by weight 1. Soy sauce 76.0 2. Black vinegar 2.0 3. Glucose 15.0 4. Sodium glutamate 3.0 5. Sanyak hot water extraction powder 1.0 6. Pepper hot water extraction powder 2.0 7. Dokudami fever Water extraction powder 1.0

(Formulation Example 14) Udon or buckwheat wt% 1. Flour or buckwheat flour 93.0 2. Salt 1.0 3. Sesame water extract 3.0 4. Bacmondou hot water extract 3.0

(Formulation Example 15) Bread Weight% 1. Wheat flour 88.5 2. Salt 0.5 3. Glucose 6.5 4. Sesame hot water extraction powder 1.5 5. Touki hot water extraction powder 1.5 6. Bakmondou pressed extraction powder 1.5

(Formulation Example 16) Cookie weight% 1. Milk 62.0 2. Whole egg 14.0 3. Sugar 15.0 4. Constarch 5.0 5. Salt 0.5 6. A: Sesame hot water extract B: Sanyak hot water extract C: Hot water extract of hot pepper D: Hot water extract of Touki E: Hot water extract of Dokudami F: Hot water extract of Bakumondou * Extract of any one of AK 3.5 7. Perfume

(Test 5) Use Effect Test The effects of a skin external preparation and a bath preparation containing an extract of sesame, sanyaku, capsicum, red pepper, prickly pear, and black bean extract of the present invention were examined.
The test was conducted using 20 panelists aged 5 to 50 years old who are suffering from skin diseases such as dry skin, rough skin, eczema, and atopic dermatitis. An appropriate amount was applied to the face over 3 months. Also, for 20 people (5 to 50 years old) who have the same skin disease on the scalp and hairline, apply the appropriate amount of the hair tonic of Formulation Example 9 to the scalp for 2 months after daily hair washing. The use test was carried out by.

Further, the bath preparation of Formulation Example 10 was also applied to 20 people aged 1 to 65 years old who suffer from skin diseases such as dry skin, rough skin, eczema, and atopic dermatitis. For a month, the children were immersed in bath water in which an appropriate amount of bath agent was dissolved once a day, and a use test was conducted. As controls, those prepared by removing the various plant extracts of the present invention from milky lotions, hair tonics, and bath preparations by the same method were used. The evaluation was performed according to the following criteria. The results are as shown in Tables 1-3, and the numbers in the table represent the number of persons. No one complained of abnormalities on the skin or scalp during the use period.

"Skin (scalp) disease improving effect" Efficacy: Redness, itching, dry skin, and rough skin due to inflammation such as eczema were improved. Somewhat effective: Redness and itching, dry skin, and rough skin due to inflammation such as eczema were slightly improved. Ineffective: No change from before use.

(Test Results) As is clear from the results shown in Tables 1-3, the use of the skin external preparation and bath preparation containing the sesame, sanyak, capsicum, touuki, dokudami, and Bakumondou extracts of the present invention caused inflammation by eczema, A favorable effect was confirmed on the improvement of skin diseases such as itching, dry skin and rough skin.

[0100]

[Table 1]

[0101]

[Table 2]

[0102]

[Table 3]

(Test 6) Use Effect Test The effect of a food containing an extract of sesame, sanyaku, capsicum, red pepper, dokudami, and Bakumondou of the present invention was actually examined. Use test is a panel of 10 people aged 5 to 50 years old who suffer from skin diseases such as dry skin, rough skin, eczema, and atopic dermatitis.
Twice in the morning and at night, five cookies of Formulation Example 16 were drunk for three months.

As a control, a cookie prepared by removing the plant extract of the present invention from a cookie in the same manner was used. The evaluation was performed according to the following criteria. The results are as shown in Table 1 and the numerical values in the table represent the number of persons. No one complained of abnormalities during the use period.

"Effect of improving skin (scalp) disease" Efficacy: Redness, itching, dry skin, and rough skin due to inflammation such as eczema were improved. Somewhat effective: Redness and itching, dry skin, and rough skin due to inflammation such as eczema were slightly improved. Ineffective: No change from before use.

(Test results) The results are shown in Table 4. As shown in Table 4, the use of the food containing the sesame, sanyaku, capsicum, toucan, tokudami, and bakumondou extracts of the present invention caused inflammation, itching, dry skin, and rough skin due to eczema. A good effect was confirmed on the improvement of skin diseases.

[0107]

[Table 4]

[0108]

According to the present invention, sesame, sanyak, pepper,
It is clear that one or more plant extracts selected from corn, dokudami, and Bakumondou have a remarkable activity of promoting fibroblast proliferation by normal cells and do not show cytotoxicity. It is safe to use internally or externally. Therefore, it can be used as an agent for promoting fibroblast proliferation, and for the purpose of promoting skin diseases such as eczema, rough skin, dry skin, atopic dermatitis and skin and dermis development, preventing skin aging, and promoting wound healing. It is effective for prevention and treatment of various traumas such as skin, scalp, oral cavity, eyes, ears, nose, anus, and genital area. Furthermore, it can be applied to all forms of preparations (pharmaceuticals, quasi-drugs, cosmetics, foods), and if used as an external preparation for skin and bath or food or drink, it will cause inflammation, itching, and skin irritation due to eczema. It can also be used for the prevention and improvement of skin and scalp such as scabs and rough skin.

[Brief description of the drawings]

FIG. 1 shows the relationship between the concentration in a culture medium and the cell growth rate when an extract of sesame, sanyak, capsicum, pepper, dokudami, and baked monster of the present invention is added.

FIG. 2 shows the relationship between the concentration in a culture medium and the cell growth rate when the extract of the present invention is added with the extract of sugarcane, dokudami and Bakumondou.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁶ Identification number Agency reference number FI Technical display location A61K 7/00 A61K 7/00 K 7/48 7/48 7/50 7/50

Claims (3)

[Claims] 1. An agent for promoting fibroblast proliferation, comprising one or more plant extracts selected from sesame, sanyak, capsicum, capsicum, dokudami and Bakumondou. 2. An external preparation for skin and a bath preparation, which comprises the fibroblast proliferation promoter according to claim 1. (3) A food or drink comprising the fibroblast proliferation promoter according to (1).