KR100561780B1 - Cosmetic compositions for anti-irritation containing ponciri fructus extracts as effective ingredients - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a tanza extract with excellent anti-irritant effect as an active ingredient, the tanza extract is an active ingredient of the present invention is included in an amount of 0.001-20.0 parts by weight.

Description

Cosmetic composition for anti-irritant containing a tanza extract {COSMETIC COMPOSITIONS FOR ANTI-IRRITATION CONTAINING PONCIRI FRUCTUS EXTRACTS AS EFFECTIVE INGREDIENTS}

The present invention relates to a cosmetic composition for anti-irritant, and more particularly, to a cosmetic composition having excellent anti-irritant effect by containing a tanza extract as an active ingredient.

Cosmetics are products used to protect the skin and cleanse beautification, but if you look closely at the cosmetic composition, ingredients different from the original skin protection purposes are used as essential to the formation of the product. Examples include solubilizers and emulsifiers, preservatives, fragrance sunscreens and other additives used to impart other efficacy and effects. These ingredients are generally known to be the main causes of inflammation, rashes and edema in the skin.

Inflammation is a series of defenses aimed at minimizing the response of a cell or tissue to any damage and restoring the damaged area to its original state. Inflammation is the cause of inflammation, including physical factors such as trauma, burns, frostbite, and radiation. There are chemical factors caused by chemicals such as acids and immunological factors caused by antibody reactions. They can also be caused by vascular or hormonal imbalances. Anti-inflammatory drugs are called anti-inflammatory drugs that work to reduce inflammation, reduce biologic reactions and symptoms in order to eliminate inflammation.

The cause of skin side effects in cosmetics is always latent, and various studies have been conducted to solve this problem. Until now, the substance used for the purpose of anti-inflammatory is nonsteroidal flufenamic acid and ibuprofen.

), Benzydamine, indometacine, and predisolone, dexamethasone, etc. (Kim Chang-jong, Pathophysiology, Hallym Co., p61-69, 1988), allantoin , Azulene, ε-aminokiproic acid, hydrocortisone (v), licorice acid and its derivatives (β-glycileic acid, glycylinic acid derivatives) are known to be effective in anti-inflammatory. , Namsan Sugar, p162, 1993), indomethacin is a substance that cannot be used in cosmetics, and hydrocortisone has a limited amount of use. Licorice acid and its derivatives are difficult to stabilize or have poor solubility. The anti-inflammatory drugs known to date have a problem in terms of skin safety and stability in formulating cosmetics. Its use is limited.

Accordingly, the present inventors have completed the present invention by finding that the tanza extract is excellent in safety for the skin and easy to stabilize the preservation when formulating cosmetics, and has a substantial effect.

Therefore, an object of the present invention is to provide an anti-irritant cosmetic composition containing a tanza extract as an active ingredient.

The Pancirus trifoliata Rafin is a deciduous shrub that grows south of Gyeonggi-do and has a strong spine of 3 to 5 centimeters in height. The leaves are regenerated, 3 leaflets, with some wings on the petiole, the lobules are stalk, obovate or oval, blunt or myo-headed, basal, 25 mm long. Flowers bloom in May, white, perennial or animate, and run one or two. The calyx leaves and petals are 5 biphytes, many stamens, and purple. Fruits are round, 3 centimeters in diameter, good in scent, but inedible, ripen in September, and seeds are long oval, 1-1.3 centimeters long. The fruit is medicinal, the seedling is used as the tree of the tangerine tree, and the sapling is welcomed as a hedge in the south (Korea Plant Book. Lee Chang-bok. Kyungmunsa, 1993).

PONCIRI FRUCTUS is the dried fruit of the tangerine tree, and its main ingredients are vitamin C and flavonoid glycosides such as neohesperidin, nargin, and rhoifolin, and methyltyramine ( m-methyl tyramine), which is known to be effective in dry stomach, edema, diarrhea, and acid value in traditional Korean medicine, and has been used for the treatment of indigestion, constipation, stomach pain, uterine contractions and disinfection.

The method of obtaining the tanza extract used in the present invention is as follows.

First, one kilogram of tanza was washed, and 3.5 kilograms of purified water and 1.5 kilograms of 1,3-butylene glycol or ethanol were added thereto, followed by extraction for 1 to 2 weeks, followed by filtration to obtain an extract of 3 kilograms. In the present invention, all the following Experimental Examples and Examples used samples extracted with 1,3-butylene glycol.

The tanza extract of the present invention is preferably used in the range of 0.001 parts by weight to 20 parts by weight relative to the total weight of the cosmetic composition, but when the tanza extract is less than 0.001 parts by weight, it does not meet the expected effect and at least 20 parts by weight of the product Problems occurred when considering the color, smell and usability in the formulation.

Cytotoxicity, allergy, anti-inflammatory and skin primary irritation evaluation experiment for the tanza extract of the present invention was carried out as follows.

Experiment 1. Cytotoxicity Test

To examine the cytotoxicity of the extract, cultured V79-4 cells [Chinese Hamster, CCL (continuous cell line), and lung tissue fibroblasts] 2-yl) -2,5-diphenyltetrazolium bromide reduction test] was performed and the MTT-formazan product was extracted with isopropyl alcohol to measure absorbance at 570 nm. In order to know the IC ₅₀ for each material, the material was diluted in 10.0%, 5.0%, 1.0% ... in the medium and incubated for 24 hours to obtain an IC ₅₀ . The results are shown in Table 1.

Cytotoxicity test results of tanza extract sample IC ₅₀ (%, W / V) Sodium Lauryl Sulfate (SLS) Tangza Extract 0.0025> 5.0

The tanza extract showed more than 2000 times lower cytotoxicity than sodium lauryl sulfate, indicating that the extract was excellent in safety.

Experiment 2. Allergy Assessment

To confirm the allergy to the extract, apply 25 μl of the sample to the mouse ear once a day for 3 days, remove the lymph node on the 4th day, isolate the lymphocytes, and incubate 24-48 hours in a 5% carbon dioxide incubator. The amount of radioisotope [ ³ H] -methylthymidine is measured. The local lymph node assay (LLNA) results indicate the lymphocyte amplification of the sample group compared to the control group, and allergens when the amplification degree (SI) of the sample is more than three times higher and the concentration tends to increase with each concentration. Considered as.

Allergy evaluation result of tanza extract sample density(%) Average cpm Amplification degree (S.I) Ethanol tanza extract tanza extract 60 50 100 2973 2132 2011 -0.7 0.7

※ -cpm (counter per minute): [ ³ H] -methylthymidine is inserted into the cell

  S.I (stimulation index): the mean cpm of the sample to the mean cpm of the control

                            Divided by

As shown in Table 2, the tanza extract of the present invention was found to have almost no allergic potential as the amplification degree (S.I) is 3 or less.

Experiment 3. Anti-inflammatory Experiment

To examine the anti-inflammatory effects of the extract, 20 rats of 5 rats were treated with 1.0% and 3.0% tanza extracts, a comparative group treated with 1.0% of indomethacin, and arachidonic acid. ) Was divided into the control group treated only to determine the average value of the mouse ear thickness. In the sample group treated with the tanza extract and the comparative group treated with indomethacin, the sample was continuously applied once a day for 4 days at 20 μl / ear, and the control group treated with only arachidonic acid was used for the same amount of solvent once a day for 4 days. After only one ethanol application, 1 hour after the last application, arachidonic acid was applied to all groups at 2 mg / ear to artificially induce edema in rat ears, and after 1 hour, the degree of ear edema was measured using a micrometer. The change in ear thickness was recorded by repeatedly measuring the rat ears of the control group and the control group three times. Anti-inflammatory effect was determined as the degree of edema inhibition based on the control group treated with arachidonic acid only. The results are shown in Table 3.

Ear thickness and inflammation inhibition rate by topical application sample density(%) Ear thickness (㎛) before arachidonic acid treatment Ear thickness (㎛) after arachidonic acid treatment % Inhibition Sample group Tanza extract tanza extract 1.0 3.0 275 288 466 453 25.1 30.2 Comparison Indomethacin 1.0 288 422 46.6 Control Arachidonic acid 2mg / ear 294 549 -

※ Inhibition rate (%) = (A-B) / A * 100

A: thickness change of control rat ear

  (Thickness of ears after arachidonic acid treatment-thickness of ears before arachidonic acid treatment)

B: Change in thickness of rat ear of sample group

  (Thickness of ears after arachidonic acid treatment-thickness of ears before arachidonic acid treatment)

As shown in Table 3, 1.0% and 3.0% of the tanza extracts showed 25.1% and 30.2%, respectively. Inhibition of edema increased the tanza extract with arachidonic acid and 99%.

Experiment 4. Primary Skin Irritation Test (Closed Wrap Test)

An aqueous solution containing 1.0% of the extract and 0.08% of sodium lauryl sulfate and 0.08% of sodium lauryl sulfate were applied to a healthy adult male and female 50 people at 24 hours with a predetermined amount (0.2 g) of each sample on the back. 4 hours after the pin chamber was removed, the skin condition was visually read and the results are shown in Table 4.

Skin primary irritation test result sample Test Index Judgment result Stimulus ++ + +- - Tanza Extract 1.0% + Sodium Lauryl Sulfate 0.08% Sodium Lauryl Sulfate 0.08% Purified Water 50 50 50 - One - -3- 5 13 6 45 33 44 0.10 0.40 0.12

※ Criteria - ; No erythema or unusual symptoms

            +-; Slightly redder than the surroundings

            +; Significantly redder than the surroundings

            ++; Severe redness and swelling than the surroundings

As shown in Table 4, 1.0% of the tanza extract was excellent in the anti-irritant effect of alleviating the stimulation of sodium lauryl sulfate 0.08% to the degree of stimulation of purified water.

In this way, the tanza extract, which is excellent in anti-irritant effect and safe for skin, can be prepared according to the use of soft cosmetics, nourishing cosmetics, nourishing cream and essence, facial cleansing pack, etc. according to the use. Presented.

Preparation Example 1

Flexible Cosmetics Parts by weight Tangerine Extract Butylene Glycol Glycerine Hyaluronic Acid Polyethylene Oleyl Ether Polyoxyethylene Cured Castor Oil Ethanol Preservative Perfume Pigment Purified Water 3.0 5.0 2.0 0.7 0.1 0.1 8.0 0.1 appropriate quantity remaining quantity

Preparation Example 2

Nutrition Parts by weight Cetostearyl Alcohol Self-emulsifying Monostearic Acid Lipophilic Monostearic Acid Beeswax Squalane Isocetyl Octanoate Dimethylpolysiloxane Polysorbate 60 Sorbitastearate Thickened Glycerin Propylene Glycol Carboxypolymer Triethanolamine Tanger Extract Preservatives 1.00 0.50 0.50 0.30 7.00 4.00 0.30 0.50 1.00 4.00 4.00 0.15 0.20 1.00 Accurate Proper Accurate

Preparation Example 3

essence Parts by weight Cetostearyl Alcohol Self-emulsifying Monostearic acid Squalane Neopentylglycol Dicaprate Dimethylpolysiloxane Polysorbate 60 Polyoxyethylene Cured Castor Oil Concentrated Glycerin Propylene Glycol Carboxypolymer Triethanolamine Tanga Extract Preservatives Flavors Pigments Purified Water 1.00 0.50 2.50 1.00 0.30 0.50 1.00 6.00 5.00 0.20 0.30 1.00 Accurate Proper Accurate

Preparation Example 4

Nutrition Cream Parts by weight Cetostearyl Alcohol Self-Emulsifying Monostearic Acid Lipophilic Monostearic Acid Beeswax Squalane Isocetyl Octanoate Neopentylglycol Dicaprate Dimethylpolysiloxane Polysorbate 60 Solbitan Stearate Thickened Glycerin Propylene Glycol Santhan Gum Triethanolamine Tangerine Extract Preservative Colorant 3.00 1.50 1.00 0.50 7.00 4.00 4.00 0.30 0.50 1.00 5.00 4.00 0.05 0.10 1.00 Accurate Proper balance

Preparation Example 5

pack Parts by weight Polyvinyl Alcohol Xanthan Gum Carboxymethyl Cellulose Polyethylene Oleyl Ether Polyoxyethylene Cured Castor Oil Ethanol Thickened Glycerin Propylene Glycol Triethanolamine Tanga Extract Preservatives Flavored Colors Purified Water 5.00 0.03 0.05 0.10 0.10 6.00 4.00 3.00 0.10 1.00 Proper Proceeds Proceeds

Cosmetic compositions prepared as described above has an excellent anti-irritant effect by the tanza extract contained as an active ingredient.

Claims (2)

Anti-irritant cosmetic composition containing as an active ingredient tanza extract. The method of claim 1, The cosmetic composition is an anti-irritant cosmetic composition, characterized in that it comprises a tanza extract in an amount of 0.001 to 20.0 parts by weight.