KR20000043943A - Preparation method of fermented feed with enzyme of microbe - Google Patents

Preparation method of fermented feed with enzyme of microbe Download PDF

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Publication number
KR20000043943A
KR20000043943A KR1019980060381A KR19980060381A KR20000043943A KR 20000043943 A KR20000043943 A KR 20000043943A KR 1019980060381 A KR1019980060381 A KR 1019980060381A KR 19980060381 A KR19980060381 A KR 19980060381A KR 20000043943 A KR20000043943 A KR 20000043943A
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enzyme
fermented
microbe
fermentation
tool
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KR1019980060381A
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Korean (ko)
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우선즉
이석민
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이석민
우선즉
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/20Inorganic substances, e.g. oligoelements
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms

Abstract

PURPOSE: A preparation method of fermented feed with enzyme of microbe is provided, which is added enzyme of microbe to leaves and stalk of crops, stool of livestock, industrial fermented wastes and etc. and then fermented CONSTITUTION: A process of preparation method of fermented feed with enzyme of microbe is made up of several processes. A first process is culturing and drying microbe and mixing the microbe with sodium chloride, calcium oxide, catalyst to be fermentive agent. A second process is put enzyme agent into tool and adding water to tool and then stirring enzyme added water. A third process is adding 1-1.5% wt of dried source melted enzyme agent to one or more kinds of sources from less 0.5mm size of crop waste, dried stool of livestock, industrial fermented wastes and then adding and mixing water. A fourth process is putting the mixed sources into tool and sealing the tool and fermenting the mixed sources at 5-40°C. 70% humidity for 48 hours-3 months.

Description

미생물 효소를 이용한 발효사료 제조방법Fermented feed manufacturing method using microbial enzyme

본 발명은 미생물 효소를 이용한 발효사료 제조방법에 관한 것으로 상세히는 동물사료를 제조함에 있어서, 농작물의 줄기, 풀, 가축의 변, 공업발효 폐기물 등에 미생물 효소를 첨가하여 발효 과정을 거쳐 동물사료를 제조하는 방법에 관한 것이다.The present invention relates to a fermented feed manufacturing method using a microbial enzyme in detail in the manufacture of animal feed, animal feed through a fermentation process by adding microbial enzymes to the stem, grass, livestock stool, industrial fermentation waste of crops, etc. It is about how to.

주지하는 바와 같이 축산업의 생산품은 인류가 생존하기 위해 필요한 단백질의 중요한 공급원으로서 축산업의 발전은 인류의 건강과 생활의 질을 향상시키는데 있어 밀접한 관계를 가지게 되며, 축산업의 발전은 사료공업의 발전에 따라 결정되는 것으로 축산업에 있어 사료공업은 불가분의 밀접한 관계인 것이다.As is well known, the production of animal husbandry is an important source of protein for human survival, and the development of animal husbandry is closely related to the improvement of human health and quality of life. It is decided that the feed industry is inseparably closely related to the livestock industry.

그러나, 세계 대부분의 국가, 지역에서 생산되는 곡물의 양은 풍부하지 못할 뿐 아니라 인구는 급격하게 늘어나고 있어 곡물의 생산은 수요를 충족시키지 못하는 형편이며, 곡물을 사료의 원료로서 이용하기는 점점 어렵게되어 축산업 발전이 제약을 받게되는 문제점이 있었다.However, the amount of grain produced in most countries and regions of the world is not only abundant, but the population is growing rapidly, and the production of grain is not enough to meet the demand, and it is increasingly difficult to use grain as a raw material for livestock production. There was a problem that development is limited.

종래에도 발효사료를 제조하는 방법들이 수종 안출되어 실용화되어 사용되고 있으나, 대부분 가격이 비싸고 절대량이 부족한 곡물을 주원료로 하여 별도의 제조설비에 의하여 제조하게 됨으로써 열 에너지 사용 등에 따른 제조원가 상승으로 경제성이 떨어지게 되어 축산업이 채산성이 맞지않게되는 문제점이 있었다.Conventionally, methods for manufacturing fermented feed have been formulated and used in practice, but most of them are manufactured by separate manufacturing facilities using grains, which are expensive and lacking in absolute amount, as a main raw material, resulting in a decrease in economic efficiency due to an increase in manufacturing cost due to the use of thermal energy. There was a problem that the livestock industry did not match the profitability.

본 발명은 상기에서와 같은 문제점에 착안하여 기존의 발효사료 들과는 달리 농업폐기물을 사료 재로써 재활용하는 방법을 제공하기 위한 것으로 여러 균종 및 발효 촉진제로 구성된 효소제를 물과 함께 농작물의 줄기, 풀, 가축의 변, 공업발효 폐기물 등에 혼합하여 용기에 담아 밀봉후 반복 뒤집기 작업이 필요 없이 일정 발효기간 동안 방치한 상태에서 발효시켜 제조하게 됨으로써 경제적이면서도 양질의 동물사료를 제조할 수 있도록 한 것이다.The present invention is to provide a method for recycling agricultural wastes as feed ash, unlike the existing fermented feeds in view of the problems as described above, the enzyme, consisting of various species and fermentation promoters, water, crops, grass, livestock It is possible to produce economical and high-quality animal feed by mixing fermentation, industrial fermentation waste, etc. and putting it in a container and fermenting it in a state left unattended for a certain period of fermentation without the need for repeated flipping after sealing.

이하 첨부된 도면 및 바람직한 실시 예에 따라 본 발명을 상세히 설명하면 다음과 같다.Hereinafter, the present invention will be described in detail with reference to the accompanying drawings and preferred embodiments.

효소제의 종균은 원핵생물계(原核生物界: Procaryote), 진핵생물계(眞核生物界: Erucaryote), 진균(眞菌: Mycology)중 임의의 일종 혹은 여러 종으로 구성되어 있다.The enzyme spawn is composed of any one or several species of prokaryotes (Procaryote), eukaryotes (Erucaryote), fungi (眞 菌 Mycology).

상기, 원핵생물계(原核生物界: Procaryote)는 방선균(放線菌: Actinomycess), 섬유소단포균(纖維素單飽菌: Cellulose mono-staturated vacteria), 유산균(乳酸菌: Lactic ferments)등을 포함한다.The prokaryotic system (Procaryote) includes Actinomycess, Actulomycetes (Cellulose mono-staturated vacteria), Lactic ferment (Lactic ferment) and the like.

상기, 진핵생물계(眞核生物界: Erucaryote)는 조류(藻類 Phycolmycete) 및 원생동물(原生動物: Protist)을 포함한다.The eukaryotes (Eucaryote) includes algae (藻類 Phycolmycete) and protists (原生動物: Protist).

상기, 진균(眞菌: Mycology)은 녹색목매(綠色木梅: Tirchoderma), 천연효모(天然酵母: Saccharomyces cerevisiae), 캔디다효모(Candida utilis), 백지매(白地梅: Deoturichum candidum), 미곡매(美曲梅: Aspergillus oryzae), 등을 포함한다.The fungus Mycology is a green hawk (Tirchoderma), natural yeast (Saccharomyces cerevisiae), Candida yeast (Candida utilis), Baekjimae (白地 梅: Deoturichum candidum)美 曲 梅: Aspergillus oryzae), and the like.

균종은 공지된 배양방법에 따라 배양한다. 1g의 혼합 균종중 균수는 100억마리이상이 되어야 균종 배양 물이 발효균 종으로써 작용한다.The species is cultured according to known culture methods. In 1g of mixed species, the number of bacteria should be more than 10 billion, and the culture of the species acts as fermented species.

발효균 종을 배양하여 건조시킨 후 염화나트륨, 촉매, 산화칼슘등의 발효 촉진제와 혼합하여 발효제로 사용한다.After culturing and drying the fermented species, it is mixed with fermentation accelerators such as sodium chloride, catalyst, calcium oxide and used as fermentation agent.

효소제를 용기에 담아 물을 가하여 잘 교반하여 10분 정도 놓아둔다.Put the enzyme in a container, add water and stir well for 10 minutes.

잘 분쇄된 농작물 폐기물(길이 0.5mm이하), 건조된 가축의 분뇨, 공업발효 폐기물등 상기한 원료의 1종 혹은 양종이상의 혼합물에 잘 용해된 효소제를 골고루 뿌리고 물을 가하여 혼합한다. 이때 습도는 70% 되도록 물을 가하거나, 건조 원료를 가하여 조정한다.The well-dissolved enzyme is mixed well with one or more mixtures of the above-mentioned raw materials, such as well-ground crop waste (less than 0.5mm in length), dried manure and industrial fermentation waste, and mixed by adding water. At this time, the humidity is adjusted to 70% by adding water or by adding a dry raw material.

상술한 제조방법에 있어 발효물중 효소제의 첨가량은 일반적으로 건조원료의 1-1.5wt% 이다.In the above-mentioned manufacturing method, the amount of the enzyme added in the fermentation product is generally 1-1.5 wt% of the dry raw material.

잘 혼합된 원료는 용기에 담아 밀봉 하에, 광선을 차단 혹은 노출, 온도는 5℃∼40℃의 조건에서 발효를 진행한다. 발효중의 습도조건은 70%를 유지한다. 상술한 발효의 최적온도는 35℃이다.The well-mixed raw materials are put in a container and sealed, and the light is blocked or exposed, and the fermentation is carried out under the conditions of 5 ° C to 40 ° C. Humidity conditions during fermentation are maintained at 70%. The optimum temperature of the above-mentioned fermentation is 35 degreeC.

상기, 제조방법상에 있어서 발효기간은 엄격하게 요구되지 않으나, 발효시 온도가 높으면 발효기간이 짧고, 온도가 낮으면 발효기간은 길게된다. 일반적으로 제일 짧게는 48시간 최장 3개월이 소요된다.In the production method, the fermentation period is not strictly required, but if the temperature is high during fermentation, the fermentation period is short, and if the temperature is low, the fermentation period is long. Generally, the shortest time is 48 hours and up to 3 months.

발효과정에서 온도는 상승하게 되고 각종 미생물이 층차(層次)적으로 활동하여 대량의 대사(代射)물질을 생성하게 된다. 예를 들면 단백질 효소(Protease), 섬유소 분해효소(Cellulase), 전분분해효소(Amylase)가 유기물 및 무기물을 분해하여 다량의 영양분을 생성한다. 또한, 발효 과정중 항균물질과 성장 촉진물질도 생성하며, 발효후의 사료는 시큼하고, 부드러우며, 달콤한 주정냄새를 풍기므로 동물의 기호 성이 높다.During fermentation, the temperature rises, and various microorganisms act in a stratified manner, producing a large amount of metabolites. For example, proteinases (Protease), fibrinase (Cellulase), starch (Amylase) break down organic and inorganic substances to produce a large amount of nutrients. In addition, antibacterial and growth promoting substances are also produced during the fermentation process, and the feed after fermentation is sour, soft and sweet smelling of alcohol, so the palatability of the animal is high.

본 발명의 발효사료는 건조 후 실온에서 보존하며, 약 12개월 보존이 가능하다.Fermented feed of the present invention is stored at room temperature after drying, can be stored for about 12 months.

이하, 본 발명의 실시방법에 대하여 설명하기로 한다. 본 발명 중 따로 설명하지 않는 한 백분비, 비율, 구분수치는 중량을 기준으로 한다.Hereinafter, an embodiment of the present invention will be described. Unless otherwise described in the present invention, percentages, ratios, and division values are based on weight.

(실시예 1)(Example 1)

방선균(放線菌:Actinomycess),섬유소단포균(纖維素單飽菌:Cellulosemono-stanturated vacteria), 유산균(乳酸菌:Lactic ferments), 조류(藻類: Phycolmycete), 원생동물(原生動物: Protist), 녹색목매(綠色木梅: Tirchoderma), 천연효모(天然酵母: Saccharomyces cerevisiae), 캔디다효모(Candida utilis), 백지매균(白地梅菌: Deotrichum candidum), 미곡매(美曲梅: Aspergillus oryzae)등을 공지된 배양방법에 따라 배양한다. 1g의 혼합 균종중 균수는 100억마리이상이 되어야 균종 배양 물이 발효균 종으로써 작용한다. 발효용 균종을 배양하여 건조시킨 후 염화나트륨, 촉매, 산화칼슘등과 혼합하여 발효제로 사용한다.Actinomyces, Cellulosemono-stanturated vacteria, Lactic ferments, Algae Phycolmycete, Protists, Green hawks Tirchoderma, natural yeast, Saccharomyces cerevisiae, Candida utilis, Deotrichum candidum, Aspergillus oryzae, etc. Incubate according to the culture method. In 1g of mixed species, the number of bacteria should be more than 10 billion, and the culture of the species acts as fermented species. After culturing and drying fermented strains, mixed with sodium chloride, catalyst, calcium oxide, etc., is used as fermentation agent.

(실시예 2)(Example 2)

구체적인 발효방법: 230㎏ 물에 1㎏의 발효제를 투입 잘 교반한다. 발효제가 잘 교반된 용액에 농작물 폐기물 분말 100㎏을 서서히 투입 균질히 섞이도록 교반한다. 잘 교반된 것을 손으로 힘껏 뭉쳐 쥐었을 때 손가락 사이로 물이 스며 나오는 정도가 가장 좋으며(습도가 70%정도) 이러한 상태가 되도록 물 또는 분말을 가감한다.Specific fermentation method: 1 kg of fermenter is added to 230 kg of water and stirred well. Slowly add 100 kg of crop waste powder to the well-stirred fermentation agent and stir to mix homogeneously. When you squeeze the well with your hands, it is best to seep out water between your fingers (humidity is around 70%) and add water or powder to this condition.

혼합 완료된 것은 통, 단지, 혹은 비닐포대에 담아 공기가 들어가지 않토록 밀봉하여 온도 25∼30℃를 유지하면서, 10∼20일 간 발효시킨다.The mixture is mixed in a barrel, jar, or plastic bag and sealed to prevent air from entering and fermented for 10 to 20 days while maintaining a temperature of 25 to 30 ° C.

(실시예 3)(Example 3)

구체적인 발효방법: 230㎏ 물에 1㎏의 발효제를 투입 잘 교반한다. 발효제가 잘 교반된 용액에 농작물 폐기물 분말 50㎏과 건조된 가축의 분뇨 혹은 공업 발효 폐기물 50㎏을 서서히 투입 균질히 섞이도록 교반한다. 잘 교반된 것을 손으로 힘껏 뭉쳐 쥐었을 때 손가락 사이로 물이 스며 나오는 정도가 가장 좋으며(습도가 70%정도) 이러한 상태가 되도록 물 또는 분말을 가감한다. 혼합 완료된 것은 통, 단지, 혹은 비닐포대에 담아 공기가 들어가지 않도록 밀봉하여 온도 25∼30℃를 유지하면서, 10∼20일간 발효시킨다.Specific fermentation method: 1 kg of fermenter is added to 230 kg of water and stirred well. 50 kg of crop waste powder and 50 kg of dried manure or industrial fermentation waste are slowly added to the well-stirred fermentation agent and stirred. When you squeeze the well with your hands, it is best to seep out water between your fingers (humidity is around 70%) and add water or powder to this condition. The mixture is mixed in a barrel, jar, or plastic bag and sealed to prevent air from entering and fermented for 10 to 20 days while maintaining a temperature of 25 to 30 ° C.

상기한 바와 같은 본 발명은 가격이 비싸고 절대량이 부족한 곡물대신 농작물 폐기물, 공업발효 폐기물, 건조된 가축분뇨 등의 폐기물을 미생물을 이용하여 발효시켜 사료로 재생산함으로써 저가에 양질의 발효사료를 얻을 수 있게되어 축산업의 고부가가치 실현이 가능케 됨은 물론 처리가 어려운 각종 폐기물을 이용하므로 써 환경오염을 방지하는 데도 크게 기여할 수 있게되는 등의 매우 유용한 효과가 있는 것이다.The present invention as described above is expensive and the absolute amount of crops instead of grain waste, industrial fermentation waste, dried livestock manure, such as waste fermentation using microorganisms to reproduce the feed to obtain a high quality fermentation feed at low cost Therefore, it is possible to realize high value-added livestock industry, and to use various wastes that are difficult to treat, which can greatly contribute to preventing environmental pollution.

Claims (1)

미생물 발효에 의하여 사료를 제조하는 발효사료 제조방법에 있어서, 방선균(放線菌:Actinomycess), 백지매균(白地梅菌: Deotrichum candidum), 섬유소단포균(纖維素單飽菌:Cellulose mono-stanturated vacteria), 유산균(乳酸菌:Lactic ferments), 조류(藻類: Phycolmycete), 원생동물(原生動物: Protist), 녹색목매(綠色木梅: Tirchoderma), 천연효모(天然酵母: Saccharomyces cerevisiae), 캔디다효모(Candida utilis), 미곡매(美曲梅: Aspergillus oryzae)로 구성된 혼합 균종을 1g당 균수가 100억마리 이상이 되도록 발효균 종을 배양하여 건조시킨 후 염화나트륨, 촉매, 산화칼슘과 혼합하여된 발효제로 써 농작물의 줄기, 풀, 가축의 변, 공업발효 폐기물 혼합물에 용해시켜 골고루 뿌리고 물을 가하여 습도가 70%되도록 맞추어 혼합시켜 용기에 담아 공기가 들어가지 않도록 밀봉하여 25℃∼30℃온도에서 10∼20일간 발효시켜 제조됨을 특징으로 하는 미생물 효소를 이용한 발효사료 제조방법.Fermented feed manufacturing method for producing feed by microbial fermentation, Actinomycetes, Deotrichum candidum, Cellulose mono-stanturated vacteria , Lactic ferments, algae (Phycolmycete), protists (protist), green bark (Tirchoderma), natural yeast (天然 酵母: Saccharomyces cerevisiae), Candida utilis ), Fermented strains were cultured and dried so that the number of bacteria per 1 gram was more than 10 billion bacteria per 1g, and then mixed with sodium chloride, catalyst, and calcium oxide. Dissolve in a mixture of stalks, grass, livestock stools, and industrial fermentation wastes, spread it evenly, add water, mix it to 70% humidity, and put it in a container to seal it out of air for 10 to 20 days at 25 ℃ ~ 30 ℃. Fermented food manufacturing method using a microbial enzyme, characterized in that the fermentation was prepared.
KR1019980060381A 1998-12-29 1998-12-29 Preparation method of fermented feed with enzyme of microbe KR20000043943A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR19990068482A (en) * 1999-05-25 1999-09-06 육인수 Aaaaa
WO2003000069A1 (en) * 2001-06-22 2003-01-03 Suk-Min Lee Method for manufacturing animal food and plastic bag type air-tight fermentation container suitable for the same
KR100553460B1 (en) * 2004-08-16 2006-02-22 경주시 농업기술센터 Method for manufacturing liquid fermented fodder mixed with loess water
CN107307212A (en) * 2017-08-10 2017-11-03 德宏景阳农业发展有限公司 Compounding pig feed based on poultry fermenting excrement

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR19990068482A (en) * 1999-05-25 1999-09-06 육인수 Aaaaa
WO2003000069A1 (en) * 2001-06-22 2003-01-03 Suk-Min Lee Method for manufacturing animal food and plastic bag type air-tight fermentation container suitable for the same
KR100553460B1 (en) * 2004-08-16 2006-02-22 경주시 농업기술센터 Method for manufacturing liquid fermented fodder mixed with loess water
CN107307212A (en) * 2017-08-10 2017-11-03 德宏景阳农业发展有限公司 Compounding pig feed based on poultry fermenting excrement

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