KR102941878B1 - 단리된 세포 배양 성분 및 액체 세포 배양 배지로부터 이를 단리하는 방법 - Google Patents

단리된 세포 배양 성분 및 액체 세포 배양 배지로부터 이를 단리하는 방법

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KR102941878B1
KR102941878B1 KR1020217027928A KR20217027928A KR102941878B1 KR 102941878 B1 KR102941878 B1 KR 102941878B1 KR 1020217027928 A KR1020217027928 A KR 1020217027928A KR 20217027928 A KR20217027928 A KR 20217027928A KR 102941878 B1 KR102941878 B1 KR 102941878B1
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liquid medium
organic solvent
composition
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KR20210125032A (ko
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데보라 리 비터필드
마이클 퀸 도헤티
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린디 바이오사이언시스, 아이엔씨.
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/10—Separation or concentration of fermentation products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02—Separating microorganisms from their culture media
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/08—Homogenizing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/12—Purification

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Sustainable Development (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
KR1020217027928A 2019-02-05 2020-02-05 단리된 세포 배양 성분 및 액체 세포 배양 배지로부터 이를 단리하는 방법 Active KR102941878B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962801225P 2019-02-05 2019-02-05
US62/801,225 2019-02-05
PCT/US2020/016743 WO2020163446A1 (en) 2019-02-05 2020-02-05 Isolated cell culture components and methods for isolating the same from liquid cell culture medium

Related Child Applications (1)

Application Number Title Priority Date Filing Date
KR1020267008174A Division KR20260053026A (ko) 2019-02-05 2020-02-05 단리된 세포 배양 성분 및 액체 세포 배양 배지로부터 이를 단리하는 방법

Publications (2)

Publication Number Publication Date
KR20210125032A KR20210125032A (ko) 2021-10-15
KR102941878B1 true KR102941878B1 (ko) 2026-03-23

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Country Status (9)

Country Link
US (2) US20220119760A1 (https=)
EP (1) EP3921082A4 (https=)
JP (2) JP2022519690A (https=)
KR (1) KR102941878B1 (https=)
CN (1) CN113661006A (https=)
AU (2) AU2020219125B2 (https=)
CA (1) CA3128204C (https=)
SG (1) SG11202107901QA (https=)
WO (1) WO2020163446A1 (https=)

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CN110087763A (zh) 2016-11-22 2019-08-02 伊勒卓菲公司 包含治疗剂或诊断剂的颗粒和悬浮液以及其使用方法
AU2018306303A1 (en) 2017-07-25 2020-02-20 Elektrofi, Inc. Formation of particles including agents
WO2019226969A1 (en) 2018-05-24 2019-11-28 Elektrofi, Inc. Particles comprising a therapeutic or diagnostic agent and suspensions and methods of use thereof
EP3917500A2 (en) 2019-01-31 2021-12-08 Elektrofi, Inc. Particle formation and morphology
CN113661006A (zh) 2019-02-05 2021-11-16 林迪生物科学公司 分离的细胞培养物组分以及用于从液体细胞培养基中分离其的方法
EP4027978A1 (en) 2019-09-13 2022-07-20 Elektrofi, Inc. Compositions and methods for the delivery of therapeutic biologics for treatment of disease
WO2021212019A1 (en) 2020-04-17 2021-10-21 Elektrofi, Inc. Methods of forming particles by continuous droplet formation and dehydration
CN112675935B (zh) * 2021-01-22 2022-03-25 中国科学院上海微系统与信息技术研究所 用于单细胞冷冻的液滴阵列芯片及液滴生成方法和应用
WO2024177927A1 (en) * 2023-02-20 2024-08-29 Lindy Biosciences, Inc. Hyaluronidase particles, compositions comprising the same and methods of making and using the same

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Also Published As

Publication number Publication date
CA3128204A1 (en) 2020-08-13
US20220119760A1 (en) 2022-04-21
JP2022519690A (ja) 2022-03-24
WO2020163446A1 (en) 2020-08-13
AU2020219125B2 (en) 2025-10-02
KR20210125032A (ko) 2021-10-15
EP3921082A4 (en) 2022-11-02
AU2025237973A1 (en) 2025-10-16
AU2020219125A1 (en) 2021-08-12
JP2025026925A (ja) 2025-02-26
EP3921082A1 (en) 2021-12-15
CA3128204C (en) 2025-06-10
US20230093954A1 (en) 2023-03-30
US11795429B2 (en) 2023-10-24
SG11202107901QA (en) 2021-08-30
CN113661006A (zh) 2021-11-16

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