KR102805266B1 - 종양-특이적 t 세포 수용체를 수득하는 방법 - Google Patents

종양-특이적 t 세포 수용체를 수득하는 방법 Download PDF

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KR102805266B1
KR102805266B1 KR1020207032466A KR20207032466A KR102805266B1 KR 102805266 B1 KR102805266 B1 KR 102805266B1 KR 1020207032466 A KR1020207032466 A KR 1020207032466A KR 20207032466 A KR20207032466 A KR 20207032466A KR 102805266 B1 KR102805266 B1 KR 102805266B1
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tumor antigen
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KR20210003794A (ko
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시앙준 조우
얀얀 한
샤오링 리앙
시허 첸
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에스와이제트 셀 테라피 코.
에이치알와이제트 (셴젠) 바이오테크 컴퍼니
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Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016145578A1 (en) 2015-03-13 2016-09-22 Syz Cell Therapy Co. Methods of cancer treatment using activated t cells
WO2019183924A1 (en) 2018-03-30 2019-10-03 Syz Cell Therapy Co. Improved multiple antigen specific cell therapy methods
WO2019196088A1 (en) 2018-04-13 2019-10-17 Syz Cell Therapy Co. Methods of obtaining tumor-specific t cell receptors
WO2019196087A1 (en) 2018-04-13 2019-10-17 Syz Cell Therapy Co. Methods of cancer treatment using tumor antigen-specific t cells
CN116298309B (zh) 2018-08-13 2024-04-02 根路径基因组学公司 成对的二分免疫受体多核苷酸的高通量克隆及其应用
WO2020206238A2 (en) 2019-04-05 2020-10-08 Rootpath Genomics, Inc. Compositions and methods for t-cell receptor gene assembly
US20220236253A1 (en) * 2019-05-31 2022-07-28 Modernatx, Inc. Expanded t cell assay
BR112022000790A2 (pt) * 2019-07-15 2022-04-12 Neogene Therapeutics B V Método para isolar genes tcr
EP4061487A4 (en) * 2019-11-19 2023-12-20 Rootpath Genomics, Inc. COMPOSITIONS AND METHODS FOR IDENTIFYING T CELL RECEPTOR
WO2021108500A1 (en) * 2019-11-26 2021-06-03 University Of Pittsburgh - Of The Commonwealth System Of Higher Education T cell receptors targeting mutations in rna splicing factors
EP4100051A2 (en) * 2020-02-07 2022-12-14 ISA Pharmaceuticals B.V. Treatment of hpv-related diseases
CN111234005B (zh) * 2020-03-13 2022-11-29 中国医学科学院皮肤病医院(中国医学科学院皮肤病研究所) 氨苯砜药物超敏反应性t细胞受体及其用途
MX2023003372A (es) * 2020-09-24 2023-05-04 Medigene Immunotherapies Gmbh Nuevos receptores prame y usos de los mismos.
US20250073333A1 (en) * 2021-07-19 2025-03-06 Board Of Regents, The University Of Texas System Peptides and engineered t cell receptors targeting fanci, rad51, and pbk antigens and methods of use
EP4405464A4 (en) * 2021-09-21 2025-12-03 Univ Chicago METHODS AND COMPOSITION USING PATIENT-DERIVED AUTOLOGOUS NEOANTIGENS FOR CANCER TREATMENT
CN116063511B (zh) * 2021-09-30 2023-10-03 北京可瑞生物科技有限公司 抗原结合蛋白及其应用
US20250025499A1 (en) * 2021-11-02 2025-01-23 Fred Hutchinson Cancer Center T cell immunotherapy for hematologic malignancies having an sf3b1 mutation
CN119677842A (zh) * 2022-03-17 2025-03-21 科士华(南京)生物技术有限公司 人工合成肿瘤浸润淋巴细胞
CN119032110A (zh) * 2022-04-07 2024-11-26 恺兴生命科技(上海)有限公司 嵌合多肽及其应用
CN116063457A (zh) * 2022-04-27 2023-05-05 恒瑞源正(广州)生物科技有限公司 Mage-a1特异性t细胞受体及其用途
KR20250024532A (ko) * 2022-05-10 2025-02-18 지노스 세라퓨틱스, 인크. 개인 맞춤형 암 에피토프 및 이의 사용 방법
CN114920824B (zh) * 2022-05-27 2023-12-05 重庆医科大学 Tcr或其抗原结合片段及其应用
WO2025011485A1 (en) * 2023-07-07 2025-01-16 The University Of Hong Kong Convergent recombination-derived hepatocellular carcinoma-specific tcrs and applications thereof
CN117567595B (zh) * 2024-01-17 2024-05-14 恒瑞源正(广州)生物科技有限公司 Mage-a4特异性t细胞受体及其用途
CN120518771A (zh) * 2024-02-20 2025-08-22 上海信谱生物医药科技有限公司 一种识别kras突变的t细胞受体及其编码序列
WO2025193663A2 (en) * 2024-03-11 2025-09-18 Affini-T Therapeutics, Inc. Compositions and methods for treating neoplasia
CN119613530B (zh) * 2024-12-13 2025-09-05 华中科技大学 一种靶向肝癌细胞甲胎蛋白抗原肽与hla-a24复合物的特异性tcr分子、tcr-t细胞及制备与应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016524914A (ja) * 2013-07-15 2016-08-22 アメリカ合衆国 抗ヒトパピローマウイルス抗原ｔ細胞の調製方法

Family Cites Families (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5703055A (en) 1989-03-21 1997-12-30 Wisconsin Alumni Research Foundation Generation of antibodies through lipid mediated DNA delivery
US5399346A (en) 1989-06-14 1995-03-21 The United States Of America As Represented By The Department Of Health And Human Services Gene therapy
HK1047109A1 (zh) 1999-10-15 2003-02-07 University Of Massachusetts 作为指定基因干预工具的rna干预轨迹基因
US6326193B1 (en) 1999-11-05 2001-12-04 Cambria Biosciences, Llc Insect control agent
AU2001275474A1 (en) 2000-06-12 2001-12-24 Akkadix Corporation Materials and methods for the control of nematodes
EP1320599A2 (en) 2000-06-28 2003-06-25 Genetics Institute, LLC Pd-l2 molecules: pd-1 ligands and uses therefor
CN1609197A (zh) * 2003-10-17 2005-04-27 上海普泛生物科技有限公司 一种用于发现肿瘤特异性抗原和肿瘤特异性抗原决定簇的高产率逆反免疫法
US20090304679A1 (en) * 2004-05-27 2009-12-10 Weidanz Jon A Antibodies as T cell receptor mimics, methods of production and uses thereof
JP5020935B2 (ja) 2005-04-08 2012-09-05 アルゴス セラピューティクス，インコーポレイティド 樹状細胞組成物および方法
EP1795599A1 (en) * 2005-12-09 2007-06-13 Schuler, Gerold, Prof. Dr. Methods for generating antigen-specific effector T cells
EP2468763B1 (en) * 2007-05-31 2014-06-18 Academisch Ziekenhuis Leiden h.o.d.n. LUMC HPV epitopes targeted by T cells infiltrating cervical malignancies for use in vaccines
CA2704232C (en) 2007-11-08 2017-05-02 Dana-Farber Cancer Institute, Inc. Stimulation of anti-tumor immunity using dendritic cell/tumor cell fusions and anti-cd3/cd28
PT2271767T (pt) 2008-04-03 2016-08-18 Cb Biotechnologies Inc Reação em cadeia de polimerase para captura de amplicões em multiplexo para amplificação de alvos múltiplos
EP2258719A1 (en) * 2009-05-19 2010-12-08 Max-Delbrück-Centrum für Molekulare Medizin (MDC) Multiple target T cell receptor
EP2567226B1 (en) 2010-05-06 2016-08-10 Adaptive Biotechnologies Corporation Monitoring health and disease status using clonotype profiles
HRP20171164T1 (hr) 2010-09-20 2017-10-20 Biontech Cell & Gene Therapies Gmbh Antigen-specifični t stanični receptori i t stanični epitopi
US9187534B2 (en) * 2011-03-11 2015-11-17 Universite De Geneve Multi-epitopic vaccine
WO2013074820A1 (en) * 2011-11-16 2013-05-23 University Of Pittsburgh - Of The Commonwealth System Of Higher Education Immunogenic tumor associated stromal cell antigen peptides and methods of their use
JP6309209B2 (ja) * 2013-05-31 2018-04-11 国立研究開発法人国立国際医療研究センター 血管平滑筋細胞の増殖を抑制する血管内皮細胞の検出方法
ES2745472T3 (es) * 2013-07-15 2020-03-02 The U S A As Represented By The Secretary Department Of Health And Human Services Office Of Tech Tra Receptores de células T anti-virus del papiloma humano 16 E6
CA3017170C (en) 2014-09-17 2021-03-23 The Johns Hopkins University Reagents and methods for identifying, enriching, and/or expanding antigen-specific t cells
CN104593325A (zh) * 2014-12-09 2015-05-06 深圳源正细胞医疗技术有限公司 诱导dc成熟并分泌高浓度促炎因子的方法
WO2016145578A1 (en) * 2015-03-13 2016-09-22 Syz Cell Therapy Co. Methods of cancer treatment using activated t cells
DK3271382T3 (da) * 2015-03-16 2020-05-04 Max Delbrueck Centrum Fuer Molekulare Medizin Helmholtz Gemeinschaft Fremgangsmåde til detektion af nye immunogene T-celleepitoper og isolering af nye antigenspecifikke T-cellereceptorer ved hjælp af et MHC-cellebibliotek
US20180171294A1 (en) 2015-03-26 2018-06-21 The Trustees Of The University Of Pennsylvania In vitro artificial lymph node method for sensitization and expansion of t cells for therapy and epitope mapping
GB201604492D0 (en) 2016-03-16 2016-04-27 Immatics Biotechnologies Gmbh Transfected t-cells and t-cell receptors for use in immunotherapy against cancers
US20170319638A1 (en) * 2016-05-06 2017-11-09 Virttu Biologics Limited Treatment of cancer
EP3468585A2 (en) 2016-06-10 2019-04-17 IO Biotech APS Calr and jak2 vaccine compositions
US9642906B2 (en) * 2016-09-16 2017-05-09 Baylor College Of Medicine Generation of HPV-specific T-cells
CN106645677B (zh) 2016-11-15 2019-08-09 恒瑞源正（上海）生物科技有限公司 体外检测肿瘤新生抗原特异性t细胞的方法、试剂盒以及肿瘤疫苗
KR20240007775A (ko) * 2016-12-08 2024-01-16 이매틱스 바이오테크놀로지스 게엠베하 T 세포 수용체 및 이를 사용하는 면역 요법
WO2019183924A1 (en) 2018-03-30 2019-10-03 Syz Cell Therapy Co. Improved multiple antigen specific cell therapy methods
WO2019196087A1 (en) 2018-04-13 2019-10-17 Syz Cell Therapy Co. Methods of cancer treatment using tumor antigen-specific t cells
WO2019196088A1 (en) 2018-04-13 2019-10-17 Syz Cell Therapy Co. Methods of obtaining tumor-specific t cell receptors

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016524914A (ja) * 2013-07-15 2016-08-22 アメリカ合衆国 抗ヒトパピローマウイルス抗原ｔ細胞の調製方法

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