KR102718353B1 - 재조합 바이러스 레플리콘 시스템 및 그의 용도 - Google Patents
재조합 바이러스 레플리콘 시스템 및 그의 용도 Download PDFInfo
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140079734A1 (en) | 2012-09-20 | 2014-03-20 | Uab Research Foundation | Methods and Compositions for Alphavirus Replicons |
| WO2014170493A2 (en) * | 2013-04-19 | 2014-10-23 | Novartis Ag | Alphavirus vector |
Family Cites Families (74)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU561343B2 (en) | 1981-10-19 | 1987-05-07 | Genentech Inc. | Human immune interferon by recombinant dna |
| DE3377363D1 (en) | 1982-03-31 | 1988-08-18 | Ajinomoto Kk | Gene coding for interleukin-2 polypeptide, recombinant dna carrying said gene, cell lines possessing the recombinant dna,and method for producing interleukin-2 using said cells |
| US4966843A (en) | 1982-11-01 | 1990-10-30 | Cetus Corporation | Expression of interferon genes in Chinese hamster ovary cells |
| US4892743A (en) | 1983-12-21 | 1990-01-09 | Schering Corporation | Novel hybrid interferon species |
| EP0165942A1 (en) | 1983-12-23 | 1986-01-02 | Monash University | PRODUCTION OF HUMAN INTERFERON-$g(a) |
| AU594014B2 (en) | 1984-03-21 | 1990-03-01 | Research Corporation Technologies, Inc. | Recombinant DNA molecules |
| JPS63500636A (ja) | 1985-08-23 | 1988-03-10 | 麒麟麦酒株式会社 | 多分化能性顆粒球コロニー刺激因子をコードするｄｎａ |
| US4810643A (en) | 1985-08-23 | 1989-03-07 | Kirin- Amgen Inc. | Production of pluripotent granulocyte colony-stimulating factor |
| US5017691A (en) | 1986-07-03 | 1991-05-21 | Schering Corporation | Mammalian interleukin-4 |
| US5116742A (en) | 1986-12-03 | 1992-05-26 | University Patents, Inc. | RNA ribozyme restriction endoribonucleases and methods |
| AU639428B2 (en) | 1988-12-01 | 1993-07-29 | Imclone Systems, Inc. | Synthetic interleukin-6 |
| US5225337A (en) | 1989-09-25 | 1993-07-06 | Innovir Laboratories, Inc. | Ribozyme compositions and methods for use |
| US5246921A (en) | 1990-06-26 | 1993-09-21 | The Wistar Institute Of Anatomy And Biology | Method for treating leukemias |
| US5780036A (en) | 1991-08-26 | 1998-07-14 | The Scripps Research Institute | Peptides for inducing cytotoxic T lymphocyte responses to hepattis B virus |
| ATE227118T1 (de) | 1993-08-02 | 2002-11-15 | Scripps Research Inst | Peptide zum induzieren einer antwort der zytotoxischen t-lymphozythen gerichetetgegen das hepatitis b-virus |
| SE9401709D0 (sv) | 1994-05-18 | 1994-05-18 | Mathilda Sjoeberg | Improved alphavirus vectors for expression of heterologous DNA |
| US5792462A (en) | 1995-05-23 | 1998-08-11 | University Of North Carolina At Chapel Hill | Alphavirus RNA replicon systems |
| US6041252A (en) | 1995-06-07 | 2000-03-21 | Ichor Medical Systems Inc. | Drug delivery system and method |
| DE19712889A1 (de) | 1997-03-27 | 1998-10-01 | Bosch Gmbh Robert | Verfahren und Vorrichtung zur Ermittlung einer den Systemdruck in einem Bremskreis beschreibenden Größe |
| US6110161A (en) | 1997-04-03 | 2000-08-29 | Electrofect As | Method for introducing pharmaceutical drugs and nucleic acids into skeletal muscle |
| US6261281B1 (en) | 1997-04-03 | 2001-07-17 | Electrofect As | Method for genetic immunization and introduction of molecules into skeletal muscle and immune cells |
| US5873849A (en) | 1997-04-24 | 1999-02-23 | Ichor Medical Systems, Inc. | Electrodes and electrode arrays for generating electroporation inducing electrical fields |
| GB0023203D0 (en) | 2000-09-21 | 2000-11-01 | Isis Innovation | Vaccination method |
| US6117660A (en) | 1997-06-10 | 2000-09-12 | Cytopulse Sciences, Inc. | Method and apparatus for treating materials with electrical fields having varying orientations |
| CA2294793A1 (fr) | 1997-06-30 | 1999-01-14 | Rhone-Poulenc Rorer S.A. | Amelioration du transfert d'acide nucleique dans le muscle strie et combinaison permettant la mise en oeuvre du procede |
| US5958060A (en) | 1998-01-02 | 1999-09-28 | General Electric Company | Method and apparatus for clock control and synchronization |
| JP2003505114A (ja) | 1998-07-13 | 2003-02-12 | ジェネトロニクス、インコーポレーテッド | パルス電場による皮膚および筋肉を標的とした遺伝子治療 |
| US6319901B1 (en) | 1998-10-15 | 2001-11-20 | Ichor Medical Systems, Inc. | Methods for prolonging cell membrane permeability |
| US20040213805A1 (en) | 1999-10-12 | 2004-10-28 | Verheije Monique Helene | Deletions in arterivirus replicons |
| WO2002020721A2 (en) | 2000-09-07 | 2002-03-14 | University Of North Carolina At Chapel Hill | Vectors derived from south african arbovirus no. 86 |
| US6538922B1 (en) | 2000-09-27 | 2003-03-25 | Sandisk Corporation | Writable tracking cells |
| DE20122302U1 (de) | 2000-11-23 | 2005-02-24 | Bavarian Nordic A/S | Variante des Modifizierten Vaccinia-Ankara-Virus |
| EP1363660A4 (en) | 2001-02-01 | 2006-06-21 | Univ Johns Hopkins | HIGHER MOLECULAR VACCINE BASED ON AUTOMPLICATIVE RNA, SUICIDE DNA OR UNDNA DNA VECTOR, WHICH LINKS ANTIGEN WITH A POLYPEPTIDE WHICH PROMOTES THE PRESENTATION OF THE ANTIGEN |
| ATE354260T1 (de) | 2001-07-19 | 2007-03-15 | Ericsson Telefon Ab L M | Verfahren und vorrichtung für die lösung der nummernübertragbarkeit am ursprungsort |
| US7245963B2 (en) | 2002-03-07 | 2007-07-17 | Advisys, Inc. | Electrode assembly for constant-current electroporation and use |
| US8209006B2 (en) | 2002-03-07 | 2012-06-26 | Vgx Pharmaceuticals, Inc. | Constant current electroporation device and methods of use |
| US6912417B1 (en) | 2002-04-05 | 2005-06-28 | Ichor Medical Systmes, Inc. | Method and apparatus for delivery of therapeutic agents |
| US7328064B2 (en) | 2002-07-04 | 2008-02-05 | Inovio As | Electroporation device and injection apparatus |
| AU2003297860A1 (en) | 2002-12-12 | 2004-07-09 | The Board Of Regents Of The University Of Texas System | Large scale production of packaged alphavirus replicons |
| EP1651666B1 (en) | 2003-07-11 | 2009-05-27 | Alphavax, Inc. | Alphavirus-based cytomegalovirus vaccines |
| US8080255B2 (en) | 2003-07-11 | 2011-12-20 | Novavax Inc. | Functional influenza virus like particles (VLPs) |
| US20050070700A1 (en) | 2003-09-30 | 2005-03-31 | Matthias Giese | Equine arteritis virus vaccine |
| DK1729848T3 (en) | 2004-03-08 | 2015-07-20 | Ichor Medical Systems Inc | IMPROVED APPARATUS FOR ELECTRICAL COMMUNICATED administration of therapeutic agents |
| CN101548014B (zh) | 2006-06-06 | 2012-07-04 | 墨尔本保健公司 | 抗病毒抗性突变的检测和应用 |
| EP2062246A4 (en) * | 2006-08-18 | 2010-09-29 | Univ North Carolina | VACCINES AGAINST CHIMARICAL VIRUSES |
| ES2993941T3 (en) | 2006-10-17 | 2025-01-14 | Inovio Pharmaceuticals Inc | Electroporation devices for electroporation of cells in mammals |
| EP2097538A4 (en) | 2006-12-07 | 2011-11-30 | Switchgear Genomics | TRANSCRIPTION REAGULATION ELEMENTS OF BIOLOGICAL PATHS, TOOLS AND METHODS |
| KR100836745B1 (ko) | 2007-01-31 | 2008-06-10 | (주)두비엘 | Ｈｂｖ 백신 및 그의 제조 방법 |
| US7850977B2 (en) * | 2007-06-21 | 2010-12-14 | Alphavax, Inc. | Promoterless cassettes for expression of alphavirus structural proteins |
| EP2222336A2 (en) | 2007-10-29 | 2010-09-01 | GENimmune N.V. | Methods and kits for inducing a ctl response using a prime boost regimen |
| NZ598000A (en) | 2009-08-07 | 2013-10-25 | Transgene Sa | Composition for treating hbv infection |
| DK2519266T3 (da) | 2009-12-31 | 2017-11-06 | Medigen Inc | Infektiøse DNA vacciner mod Chikungunya virus |
| DK3243526T3 (da) | 2010-07-06 | 2020-02-17 | Glaxosmithkline Biologicals Sa | Levering af rna til at udløse flere immunsignalveje |
| EP2655621B1 (en) | 2010-12-20 | 2018-05-23 | The General Hospital Corporation | Polycomb-associated non-coding rnas |
| KR101942372B1 (ko) | 2011-02-11 | 2019-04-12 | 더 트러스티스 오브 더 유니버시티 오브 펜실바니아 | B형 간염 바이러스 코어 단백질을 암호화하는 핵산 분자 및 이를 포함하는 백신 |
| EP3473296B1 (en) | 2011-06-28 | 2020-08-05 | Inovio Pharmaceuticals, Inc. | A minimally invasive dermal electroporation device |
| TWI623618B (zh) | 2011-07-12 | 2018-05-11 | 傳斯堅公司 | Ｈｂｖ聚合酶突變體 |
| EP3178505B1 (en) | 2012-12-13 | 2018-05-02 | Panasonic Healthcare Holdings Co., Ltd. | Medicament injection device |
| CN105473157A (zh) | 2013-08-21 | 2016-04-06 | 库瑞瓦格股份公司 | 组合疫苗 |
| WO2016020538A1 (en) | 2014-08-08 | 2016-02-11 | Transgene Sa | Hbv vaccine and antibody combination therapy to treat hbv infections |
| US10166288B2 (en) | 2014-10-01 | 2019-01-01 | The Trustees Of The University Of Pennsylvania | Vaccines having an antigen and interleukin-21 as an adjuvant |
| IL254817B2 (en) | 2015-04-08 | 2023-12-01 | Novartis Ag | Cd20 therapies, cd22 therapies, and combination therapies with a cd19 chimeric antigen receptor (car) - expressing cell |
| DK3294326T3 (da) | 2015-05-15 | 2021-05-31 | Curevac Ag | Prime-boost-regimer indbefattende indgivelse af mindst én mrna-konstruktion |
| CN116196403A (zh) | 2015-08-03 | 2023-06-02 | 美国卫生和人力服务部 | Brachyury缺失突变体、编码该缺失突变体的非酵母载体及其用途 |
| WO2017059902A1 (en) * | 2015-10-07 | 2017-04-13 | Biontech Rna Pharmaceuticals Gmbh | 3' utr sequences for stabilization of rna |
| EP3436139B1 (en) | 2016-03-28 | 2025-04-30 | PapiVax Biotech, Inc. | METHOD AND APPARATUS FOR ADMINISTERING THERAPEUTIC AGENTS |
| WO2017176319A1 (en) | 2016-04-06 | 2017-10-12 | University Of Washington | Therapeutic vaccine for hepatitis b virus (hbv) using the hbv core antigen |
| JP7250520B2 (ja) | 2016-04-13 | 2023-04-03 | ヤンセン ファーマシューティカルズ，インコーポレーテッド | 組換えアルテリウイルスレプリコン系およびその使用 |
| AU2017347725B2 (en) * | 2016-10-17 | 2024-01-04 | Janssen Pharmaceuticals, Inc. | Recombinant virus replicon systems and uses thereof |
| EP3548625B1 (en) | 2016-12-05 | 2024-06-26 | Janssen Pharmaceuticals, Inc. | Compositions and methods for enhancing gene expression |
| JP6613399B2 (ja) | 2017-06-05 | 2019-12-04 | 株式会社ビークル | Ｈｂｖに対する免疫応答を生じさせるために用いられるウイルス様粒子 |
| CN111542340B (zh) | 2017-11-16 | 2023-12-19 | 华盛顿大学 | 治疗性乙型肝炎病毒(hbv)疫苗 |
| JOP20200153A1 (ar) | 2017-12-19 | 2022-10-30 | Janssen Sciences Ireland Unlimited Co | لقاحات التهاب الكبد الفيروسي b (hbv) واستخداماته |
| JP7494117B2 (ja) * | 2018-01-19 | 2024-06-03 | ヤンセン ファーマシューティカルズ，インコーポレーテッド | 組換えレプリコン系を使用する免疫応答の誘導および増強 |
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- 2017-10-03 US US15/723,658 patent/US11364310B2/en active Active
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140079734A1 (en) | 2012-09-20 | 2014-03-20 | Uab Research Foundation | Methods and Compositions for Alphavirus Replicons |
| WO2014170493A2 (en) * | 2013-04-19 | 2014-10-23 | Novartis Ag | Alphavirus vector |
Non-Patent Citations (1)
| Title |
|---|
| J. Virol., Vol.83, No.17, pp.8327-8339(2009.06.10.)* |
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| US20230126773A1 (en) | 2023-04-27 |
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| WO2018075235A1 (en) | 2018-04-26 |
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| EP3526332A1 (en) | 2019-08-21 |
| MX2019004499A (es) | 2019-11-18 |
| KR20190082226A (ko) | 2019-07-09 |
| ES2993107T3 (en) | 2024-12-23 |
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